A composition for improving or promoting the absorption of whey protein and its application

Abstract

The present invention discloses a composition for improving or promoting the absorption of whey protein. The composition is composed of the following raw materials in parts by weight: whey protein, vitamin D3, and black pepper extract, wherein the black pepper extract is obtained by a special extraction process, as well as a preparation containing the composition for improving or promoting the absorption of whey protein and a sports nutrition food for promoting muscle synthesis. According to the composition for improving or promoting the absorption of whey protein of the present invention, whey protein, vitamin D3, and black pepper extract are compounded in a specific ratio. The test results prove that when the three extracts of whey protein, vitamin D3, and black pepper extract are compounded in a certain ratio, the muscle-building effect can be significantly increased, and there is a synergistic effect, making the composition of the present invention have an excellent muscle-building effect.

Description

[0001] Citation of Related Applications

[0002] This application claims priority to a Chinese patent application titled "A Composition for Promoting Muscle Synthesis, Its Preparation Method and Use", with application number 202211655835.1, filed on December 22, 2022. The content of this prior application is hereby incorporated by reference in its entirety. Technical Field

[0003] The present invention belongs to the field of sports nutrition foods, and relates to a composition for improving or promoting the absorption of whey protein. Specifically, it relates to a composition for improving or promoting the absorption of whey protein, which is composed of whey protein, vitamin D3 and black pepper extract, and its preparation method, as well as a preparation containing the composition for improving or promoting the absorption of whey protein, and the use of the composition for improving or promoting the absorption of whey protein and the preparation containing the composition for improving or promoting the absorption of whey protein in the preparation of muscle-building sports nutrition products. Background Art

[0004] Muscles are crucial for the health of the body. Whether for athletes or the general public, there is a need to promote muscle mass and maintain muscle volume. Exercise combined with reasonable nutritional supplementation is an effective way to promote muscle growth. After exercise is the golden period for protein supplementation, which has a great impact on muscle generation, metabolism and the repair of injured muscles. At this time, the supplementation of high-quality protein can provide the body with a large amount of essential amino acids, especially leucine, which can not only provide raw materials for muscle protein synthesis, but also promote insulin secretion and stimulate muscle protein synthesis. Leucine can also directly stimulate mTOR (a key factor in protein synthesis) to initiate muscle protein synthesis.

[0005] Whey protein is a type of protein extracted from milk using modern production technology. Whey protein is rich in essential amino acids and leucine, has a low fat content and a high digestibility, and is a commonly used protein supplement for athletes. Intense exercise can lead to a decline in the body's digestive function, which may be related to reasons such as dehydration caused by exercise and insufficient blood flow to the digestive system due to a large amount of blood concentrating in skeletal muscles. Although whey protein is an easily digestible protein, some people still experience indigestion, abdominal distension, etc. after consuming whey protein after exercise.

[0006] Currently, in muscle-building nutritional foods for athletes, the ingredients are relatively complex, but the muscle-building effect of single-component whey protein is not ideal. Therefore, how to develop a sports nutrition food that provides new choices for muscle-building athletes, has relatively simple ingredients, and has an obvious muscle-building effect is a technical problem that needs to be urgently solved. Summary of the Invention

[0007] The object of the present invention is to provide a composition that can promote the digestion and absorption of whey protein in the intestine, rapidly increase the concentration of leucine and essential amino acids in the blood, stimulate and promote human muscle synthesis, and cause no harm to physical health. The composition for improving or promoting the absorption of whey protein is especially designed for athletes.

[0008] To achieve the object of the present invention, the novel composition for improving or promoting the absorption of whey protein according to the present invention is composed of the following raw materials in parts by weight: 30 - 98 parts of whey protein, 0.001 - 0.06 part of vitamin D3, and 0.002 - 2 parts of black pepper extract.

[0009] The preparation method of the black pepper extract includes the following steps:

[0010] Crush black pepper, reflux and extract it twice with 95% ethanol at 6 times the weight of black pepper for 1.5 hours each time, filter, combine the filtrates, vacuum concentrate the filtrates to a paste to form a crude paste extract, freeze-dry the crude paste extract at -45°C, and then crush it with a ball mill to obtain a powder of the crude paste extract;

[0011] Wash the powder of the crude paste extract twice with a n-hexane washing solution at 25 - 60°C, dissolve it with 95% ethanol at 4 times the weight of the black pepper extract, filter it with activated carbon, and vacuum concentrate to obtain a concentrated filtrate;

[0012] Freeze-dry the concentrated filtrate obtained by vacuum concentration at -15°C to form a freeze-dried product, and grind the freeze-dried product at -15°C to obtain black pepper extract particles with an average particle size of 15 - 30 μm.

[0013] More preferably, the novel composition for improving or promoting the absorption of whey protein according to the present invention is composed of the following raw materials in parts by weight: 60 - 96 parts of whey protein, 0.004 - 0.02 part of vitamin D3, and 0.006 - 1 part of black pepper extract.

[0014] Most preferably, the novel composition for improving or promoting the absorption of whey protein according to the present invention is composed of the following raw materials in parts by weight: 80 - 96 parts of whey protein, 0.002 - 0.013 part of vitamin D3, and 0.01 - 0.5 part of black pepper extract.

[0015] The raw material whey protein in the composition for improving or promoting the absorption of whey protein according to the present invention includes concentrated whey protein, isolated whey protein, and / or hydrolyzed whey protein, and vitamin D3 can be commercially available.

[0016] In the method for preparing the black pepper extract according to the present invention, the dosage of the extraction solvent is calculated according to the mass-volume ratio with black pepper, and the unit can be g / mL. In the present invention, the percentage of the ethanol solution represents the volume percentage.

[0017] Another object of the present invention is to provide a method for preparing the composition for improving or promoting the absorption of whey protein, the preparation method comprising: weighing each raw material, sieving, and mixing evenly to obtain the composition.

[0018] Another object of the present invention is to provide a preparation comprising the composition for improving or promoting the absorption of whey protein, the preparation comprising the composition for improving or promoting the absorption of whey protein and a pharmaceutically acceptable carrier or excipient.

[0019] Furthermore, the preparation comprises 100 parts by weight of the composition for improving or promoting the absorption of whey protein and 0.01 - 3500 parts by weight of a pharmaceutically acceptable carrier or excipient.

[0020] The pharmaceutically acceptable carrier or excipient is preferably selected from one or more of xylitol, erythritol, sorbitol, isomaltitol, isomaltose, maltitol oligosaccharide, granulated sugar, ethanol, microcrystalline cellulose, lactose, maltodextrin, starch, hydroxypropyl methylcellulose, sodium carboxymethylcellulose, magnesium stearate, sweeteners and edible flavors, etc.

[0021] The preparation according to the present invention can be in dosage forms such as tablets, capsules, powders or granules.

[0022] The parts by weight in the present invention can be weight units well-known in the medical field such as μg, mg, g, kg, etc.

[0023] Another object of the present invention is to provide the use of the composition for improving or promoting the absorption of whey protein or the preparation comprising the composition for improving or promoting the absorption of whey protein in the preparation of muscle-building sports nutrition foods.

[0024] Beneficial effects

[0025] For the novel composition for improving or promoting the absorption of whey protein according to the present invention, whey protein, vitamin D3 and black pepper extract are compounded in a specific ratio. Among them, whey protein is rich in essential amino acids and leucine, which can provide a large amount of essential amino acids for the body, especially leucine. This can not only provide raw materials for muscle protein synthesis, but also promote insulin secretion and stimulate muscle protein synthesis. Leucine can also directly stimulate mTOR (a key factor in protein synthesis) to initiate muscle protein synthesis. Vitamin D3 has multiple biological effects on muscles, and it has a synergistic effect with leucine in enhancing protein anabolic metabolism, which has potential benefits for muscle function.

[0026] While vitamin D3 has little effect on muscle protein synthesis and muscle strength when used alone, it can effectively improve muscle status when combined with the intake of proteins rich in essential amino acids.

[0027] Black pepper extract can not only improve the bioavailability of various nutrients. The components in black pepper extract may promote the absorption and utilization of substances such as proteins in three aspects: enhancing the activity of digestive enzymes, promoting the absorption of intestinal epithelial cells, and enhancing metabolism through thermogenesis.

[0028] The experiments of the present invention prove that when whey protein, vitamin D3 and black pepper extract are compounded in a certain proportion, they have a synergistic effect and jointly promote muscle synthesis, making the composition of the present invention have excellent muscle-building effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for the description of the specific embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0030] Figure 1 A comparison chart showing the effects of ingesting the composition for improving or promoting the absorption of whey protein according to the present invention and the control group food on the serum leucine content;

[0031] Figure 2 A comparison chart showing the effects of ingesting the composition for improving or promoting the absorption of whey protein according to the present invention and the control group food on the serum essential amino acid content;

[0032] Figure 3 A comparison chart showing the effects of ingesting the composition for improving or promoting the absorption of whey protein according to the present invention and the control group food on the serum insulin content;

[0033] Figure 4 A comparison chart showing the effects of ingesting the composition for improving or promoting the absorption of whey protein according to the present invention and the control group food on the muscle protein fractional synthesis rate.

[0034] In each figure, * indicates a significant difference compared with group A (P < 0.05); # indicates a significant difference compared with group B (P < 0.05), & indicates a significant difference compared with group C (P < 0.05), ￥ indicates a significant difference compared with group E (P < 0.05), and @ indicates a significant difference compared with group F (P < 0.05). DETAILED DESCRIPTION OF THE EMBODIMENTS

[0035] The present invention will be described in detail below. Before the description, it should be understood that the terms used in this specification and the appended claims should not be construed as limited to the general meaning and dictionary meaning, but should be interpreted according to the meaning and concept corresponding to the technical aspects of the present invention on the basis of the principle that allows the inventor to appropriately define the terms for the best interpretation. Therefore, the description presented here is only a preferred example for illustrative purposes and is not intended to limit the scope of the present invention. Thus, it should be understood that other equivalent ways or improved ways can be obtained without departing from the spirit and scope of the present invention.

[0036] In the composition for improving or promoting the absorption of whey protein according to the present invention, three active ingredients, namely whey protein, vitamin D3, and black pepper extract, are used. Among them, the black pepper extract is treated by a special process to most effectively and maximally retain the key ingredient piperine in black pepper.

[0037] In the preparation method of the black pepper extract, first, alcohol extraction is carried out with 95% ethanol to extract the key ingredient piperine as much as possible. Then, the obtained paste-like crude extract is freeze-dried at -45°C, and then pulverized with a ball mill to obtain a crude extract powder. Through this freeze-drying step, piperine can be effectively protected from being destroyed in the later steps such as pulverization. Then, it is dissolved in ethanol again, filtered with activated carbon, and the filtrate is vacuum-concentrated to ensure the purity of piperine. Then, it is freeze-dried again at -15°C, and then ground at the same temperature to obtain black pepper extract particles with an average particle size of 15 to 30 μm. The temperature during the second freeze-drying should not be too low because after two alcohol extraction treatments with ethanol dissolution, the piperine has a high purity and its molecular structure integrity should be maintained as much as possible. Therefore, the temperature of the second freeze-drying should not be too low, and appropriately increasing the temperature is also beneficial for energy conservation.

[0038] The following examples are only listed as examples of the implementation schemes of the present invention and do not constitute any limitation to the present invention. Those skilled in the art can understand that modifications within the scope not deviating from the essence and concept of the present invention fall within the protection scope of the present invention. Unless otherwise specified, the reagents and instruments used in the following examples are all commercially available products.

[0039] The content of vitamin D3 used in the present invention is 100,000 IU / g.

[0040] Example 1. Preparation of a powder of the composition for improving or promoting the absorption of whey protein

[0041] The preparation method of the powder of the composition for improving or promoting the absorption of whey protein in this example includes the following steps:

[0042] 1. Composition formula for improving or promoting the absorption of whey protein: 98 g of whey protein, 0.01 g of vitamin D3, 1 g of black pepper extract, 0.9 g of isomaltulose, 0.1 g of edible flavor.

[0043] Among them, the black pepper extract is prepared as follows: Crush black pepper, reflux extract with 95% ethanol twice, each time for 1.5 hours, filter, combine the filtrates and vacuum concentrate to a paste to obtain a crude paste extract. Freeze-dry the crude paste extract at -45°C, then crush it with a ball mill to obtain a crude extract powder. Then, dissolve the crude paste extract powder in 95% ethanol at 25°C to 60°C, filter with activated carbon, vacuum concentrate the filtrate, and then freeze-dry the concentrated filtrate again at -15°C and grind it at -15°C to obtain a black pepper extract with an average particle size of 15 to 30 μm.

[0044] 2. Preparation process of the composition for improving or promoting the absorption of whey protein

[0045] 1) Weigh the raw materials according to the formula ratio in step 1: whey protein, vitamin D3, black pepper extract, and pass through a 40-mesh sieve respectively for standby.

[0046] 2) Weigh the excipients according to the formula ratio: isomaltulose and edible flavor, and mix the above raw materials and excipients evenly by the equal increment addition method and bag them.

[0047] 3. Product specification of the composition for improving or promoting the absorption of whey protein in this example: 30 g per bag.

[0048] Example 2. Preparation of the granule of the composition for improving or promoting the absorption of whey protein

[0049] The preparation method of the granule of the composition for improving or promoting the absorption of whey protein in this example includes the following steps:

[0050] 1. Composition formula for improving or promoting the absorption of whey protein: 30 g of whey protein, 0.06 g of vitamin D3, 0.01 g of black pepper extract, 30 g of maltodextrin, 40 g of maltitol oligosaccharide.

[0051] Among them, the black pepper extract is prepared as follows: Crush black pepper, reflux extract with 95% ethanol twice, each time for 1.5 hours, filter, combine the filtrates and vacuum concentrate to a paste to obtain a crude paste extract. Freeze-dry the crude paste extract at -45°C, then crush it with a ball mill to obtain a crude extract powder. Then, dissolve the crude extract powder in 95% ethanol at 25°C to 60°C, filter with activated carbon, vacuum concentrate the filtrate, then freeze-dry it again at -15°C, and then grind it at the same temperature to obtain black pepper extract granules with an average particle size of 15 to 30 μm.

[0052] 2. Preparation process of composition for improving or promoting whey protein absorption

[0053] 1) Weigh the raw materials respectively according to the formula ratio in step 1: whey protein, vitamin D3, black pepper extract, and pass them through a 40-mesh sieve respectively for standby.

[0054] 2) Weigh the excipients maltodextrin and maltitol according to the formula ratio, mix the above-mentioned raw materials and excipients evenly by the equal increment addition method, use 50% ethanol as the binder to make soft materials, make the soft materials into granules in a swing granulator with a 14-mesh sieve, place the granules in a multi-functional fluidized bed dryer, dry them at 50 °C for about 40 min, control the moisture content of the granules at 5%, then size the granules and pack them into bags.

[0055] 3. Product specification of the granule of the composition for improving or promoting whey protein absorption in this example: 30 g per bag.

[0056] Example 3. Preparation of tablets of composition for improving or promoting whey protein absorption

[0057] The preparation method of the tablets of the composition for improving or promoting whey protein absorption in this example includes the following steps:

[0058] 1. Formula of composition for improving or promoting whey protein absorption: 90 g of whey protein, 0.02 g of vitamin D3, 0.5 g of black pepper extract, 370 g of isomaltulose, 40 g of magnesium stearate.

[0059] Among them, the black pepper extract is prepared as follows: crush black pepper, reflux and extract it twice with 95% ethanol for 1.5 hours each time, filter, combine the filtrates and concentrate them in vacuo to a paste, freeze-dry the paste-like crude extract at -45 °C, then crush it with a ball mill to obtain a crude extract powder, then dissolve it with 95% ethanol at 25 °C to 60 °C, filter it with activated carbon, concentrate the filtrate in vacuo, then freeze-dry it again at -15 °C, and then grind it at the same temperature to obtain black pepper extract particles with an average particle size of 15 to 30 μm.

[0060] 2. Preparation process of composition for improving or promoting whey protein absorption

[0061] 1) Weigh the raw materials respectively according to the formula ratio in step 1: whey protein, vitamin D3, black pepper extract, for standby.

[0062] 2) Weigh isomaltulose and magnesium stearate according to the formula ratio. Mix the above-mentioned raw and auxiliary materials (except magnesium stearate) evenly by the equal increment addition method. Use ethanol with a volume percentage concentration of 50% as the binder to prepare soft materials. Make the soft materials into granules in a rocking granulator with a 14-mesh sieve. Place the granules in a multi-functional fluidized bed dryer and dry them at 50 °C for about 40 min. Control the moisture content of the granules at 5%. Screen the granules, then add magnesium stearate to the granules, mix evenly, press tablets, coat with film coating, and pack.

[0063] 3. Specification of the composition tablet product for improving or promoting the absorption of whey protein in this example: 2.0 g per tablet.

[0064] Example 4. Preparation of the composition for improving or promoting the absorption of whey protein

[0065] The preparation method of the composition powder for improving or promoting the absorption of whey protein in this example includes the following steps:

[0066] 1. Composition formula for improving or promoting the absorption of whey protein: 900 g of whey protein, 0.125 g of vitamin D3, 5.0 g of black pepper extract, 94 g of maltodextrin, and 1.0 g of edible essence.

[0067] Among them, the black pepper extract is prepared as follows: Crush black pepper, reflux and extract it twice with 95% ethanol for 1.5 hours each time. Filter, combine the filtrates and concentrate them in vacuo to a paste to obtain a crude paste extract. Freeze-dry the crude paste extract at -45 °C, then crush it with a ball mill to obtain a crude extract powder. Then dissolve the crude extract powder in 95% ethanol at 25 to 60 °C, filter with activated carbon, concentrate the filtrate in vacuo. Then, freeze-dry the concentrated filtrate again at -15 °C, and grind it at the same temperature to obtain black pepper extract particles with an average particle size of 15 to 30 μm.

[0068] 2. Preparation process of the composition for improving or promoting the absorption of whey protein

[0069] 1) Weigh the raw materials according to the formula ratio in step 1: whey protein, vitamin D3, and black pepper extract, and pass them through a 40-mesh sieve respectively for standby.

[0070] 2) Weigh the auxiliary materials according to the formula ratio: maltodextrin and edible essence. Mix the above-mentioned raw and auxiliary materials evenly by the equal increment addition method and put them into a barrel.

[0071] 3. Specification of the composition powder product for improving or promoting the absorption of whey protein in this example: 1.0 kg per bag.

[0072] Example 5. Preparation of the composition powder for improving or promoting the absorption of whey protein

[0073] The preparation method of the composition powder for improving or promoting the absorption of whey protein in this embodiment includes the following steps:

[0074] 1. Formula of the composition powder for improving or promoting the absorption of whey protein: 350 g of whey protein, 0.2 g of vitamin D3, 10 g of black pepper extract, 400 g of maltodextrin, 200 g of maltitol oligosaccharide, 39 g of glucose, and 1.0 g of edible essence.

[0075] Among them, the black pepper extract is prepared as follows: Black pepper is crushed, refluxed and extracted twice with 95% ethanol for 1.5 hours each time, filtered, and the filtrates are combined and vacuum concentrated to a paste to obtain a crude paste extract. The crude paste extract is freeze-dried at -45°C, then pulverized with a ball mill to obtain a crude extract powder. Then, the crude extract powder is dissolved in 95% ethanol at 25 to 60°C, filtered with activated carbon, the filtrate is vacuum concentrated, then freeze-dried again at -15°C, and then ground at the same temperature to obtain black pepper extract particles with an average particle size of 15 to 30 μm.

[0076] 2. Preparation process of the composition for improving or promoting the absorption of whey protein

[0077] 1) Weigh the raw materials according to the formula ratio in step 1: whey protein, vitamin D3, and black pepper extract, and pass them through a 40-mesh sieve respectively for standby.

[0078] 2) Weigh the auxiliary materials maltodextrin, maltitol oligosaccharide, glucose, and edible essence according to the formula ratio, and mix the above-mentioned raw and auxiliary materials evenly by the equal increment addition method, and then put them into a barrel.

[0079] 3. Product specification of the composition for improving or promoting the absorption of whey protein in this embodiment: 1.0 kg per bag.

[0080] Example 6. Preparation of the composition tablets for improving or promoting the absorption of whey protein

[0081] The preparation method of the composition tablets for improving or promoting the absorption of whey protein in this embodiment includes the following steps:

[0082] 1. Formula: 98 g of whey protein, 0.06 g of vitamin D3, 2 g of black pepper extract, 260 g of sorbitol, 100 g of microcrystalline cellulose, and 40 g of magnesium stearate.

[0083] Among them, the black pepper extract is prepared as follows: Black pepper is crushed, refluxed and extracted twice with 95% ethanol for 1.5 hours each time, filtered, and the filtrates are combined and concentrated in vacuo to a paste to obtain a crude paste extract. The crude paste extract is freeze-dried at -45°C, then pulverized with a ball mill to obtain a crude extract powder. Then, the crude extract powder is dissolved in 95% ethanol at 25 to 60°C, filtered with activated carbon, and the filtrate is concentrated in vacuo. Then, the filtrate is freeze-dried again at -15°C, and then ground at the same temperature to obtain black pepper extract particles with an average particle size of 15 to 30 μm.

[0084] 2. Preparation process of the composition tablet for improving or promoting whey protein absorption

[0085] 1) Weigh the raw materials according to the formulation ratio in step 1: whey protein, vitamin D3, black pepper extract, and set aside.

[0086] 2) Weigh the excipients according to the formulation ratio: sorbitol, microcrystalline cellulose, magnesium stearate. Mix the above-mentioned raw materials and excipients (except magnesium stearate) evenly by the equal increment method, use ethanol with a volume percentage concentration of 50% as the binder to make soft materials, and make the soft materials into granules in a rocking granulator with a 14-mesh sieve. Place the granules in a multi-functional fluidized bed dryer and dry them at 50°C for about 40 min, control the moisture content of the granules at 5%, size the granules, then add magnesium stearate to the granules, mix evenly, press tablets, coat with film coating, and package.

[0087] 3. Specification of the composition tablet product for improving or promoting whey protein absorption in this example: 2.0 g per tablet.

[0088] Preparation of the powder in Comparative Example 1

[0089] Prepare the powder according to the relevant formulation and preparation process in Example 1, except that the black pepper extract is prepared as follows:

[0090] Reflux and extract with 70% ethanol. For the first reflux extraction, use 8 times the amount of 70% ethanol to extract for 2 h, and for the second reflux extraction, use 6 times the amount of 70% ethanol to extract for 1.5 h. Filter the extract, combine the filtrates, concentrate under reduced pressure (vacuum degree -0.06 to -0.08 Mpa) to a relative density of 1.1 - 1.15 (70°C - 80°C), and spray dry to obtain the black pepper extract.

[0091] Preparation of the powder in Comparative Example 2

[0092] Reflux extraction was carried out with ethanol at a concentration of 70%. For the first reflux extraction, 8 times the amount of 70% ethanol was used for 2 hours, and for the second reflux extraction, 6 times the amount of 70% ethanol was used for 1.5 hours. The extraction solution was filtered, and the filtrates were combined and concentrated under reduced pressure (vacuum degree: -0.06 to -0.08 Mpa) to a relative density of 1.1 - 1.15 (70°C - 80°C), and then spray-dried to obtain black pepper extract.

[0093] Experimental Example 1. Test on the application effect of the composition for improving or promoting whey protein absorption 1.1 Research subjects:

[0094] Forty-two male college students from Beijing Sport University with exercise habits were selected as research subjects. None of the subjects had taken other sports nutrition supplements one month before the experiment. The subjects signed an informed consent form before the experiment and were randomly divided into a whey protein group (Group A, given whey protein powder), a whey protein + vitamin D3 group (Group B, given a composition of whey protein powder and vitamin D3), a whey protein + black pepper extract group (Group C, given a composition of whey protein powder and black pepper extract obtained according to the preparation method of the present invention), a whey protein + vitamin D3 + black pepper extract group (Group D, given the powder prepared in Example 1), a control group 1 (Group E, given the powder prepared in Comparative Example 1), and a control group 2 (Group F, given the powder prepared in Comparative Example 2).

[0095] Table 1 Statistical information on the basic conditions of the subjects

[0096] Grouping Age (yr) Height (cm) Weight (kg) A (n = 7) 21.1±0.67 177.7±8.1 71.1±11.9 B (n = 7) 20.9±0.74 176.8±6.3 70.4±7.8 C (n = 7) 21.5±0.51 176.5±7.7 71.3±8.2 D (n = 7) 21.3±0.81 177.5±5.7 71.8±10.1 E (n = 7) 20.8±0.71 176.9±9.3 70.9±9.8 F (n = 7) 21.3±0.39 177.1±8.5 71.5±8.9

[0097] 1.2 Dietary control and nutritional supplement

[0098] During the experiment, all subjects were required to eat the same food at the same place and at the same time, and have three meals a day. The calorie intake was calculated at 45 kcal per kilogram of body weight per day, and the calorie ratio of the three major energy substances was 65% for carbohydrates, 15% for protein, and 20% for fat. The experimental diet started one week before the experiment, and there were no behaviors such as drinking alcohol, caffeine intake, or staying up late.

[0099] After the experiment started, nutritional supplements were taken every day. Group A: whey protein; Group B: whey protein + vitamin D3, with a protein content of 75%; Group C: whey protein + black pepper extract; Group D: the powder of Example 1; Group E: the powder of Comparative Example 1; Group F: the powder of Comparative Example 2. The protein content of the nutritional supplements in each group was 75%, and 40 g was taken each time, brewed with warm water, and taken 30 minutes after the end of training on training days.

[0100] 1.3 Research methods:

[0101] 1.3.1 Experimental design

[0102] The experiment was divided into two parts:

[0103] ① To study the effects of single - dose intake of whey protein composition on the digestion and absorption of whey protein and the rate of skeletal muscle protein synthesis

[0104] Three days before the start of the test, no strenuous exercise was carried out. Fasting started at 22:00 on the day before the start of the test. At 8:00 am the next day, venous blood was drawn and muscle biopsy samples were taken. Then, 40 g of nutritional products labeled with L - [cyclo - 13 C6]phenylalanine were ingested respectively: whey protein (Group A), whey protein + vitamin D3 (Group B), whey protein + black pepper extract group (Group C), the powder group of Example 1 (Group D), the powder group of Comparative Example 1 (Group E), and the powder group of Comparative Example 2 (Group F). Then, venous blood was drawn every 30 min within 120 min, and once every 60 min from 120 min to 240 min. Muscle biopsy samples were collected at the 120th min and the 240th min. All three muscle biopsy samples were taken from the middle area of the lateral femoral muscle.

[0105] ② To study whether 4 - week supplementation of the whey protein composition of the present invention combined with resistance exercise can improve the body composition of college students with exercise habits and enhance muscle strength.

[0106] After completing Experiment ①, the subjects rested for one day. Height, weight, and body composition were measured. Squat and bench press 1RM tests, and force platform vertical jump (CMJ) height tests were carried out using GYMAWARE.

[0107] According to the 1RM test results, a training plan was formulated: 70% 1RM for squats, 4 sets, 6 - 8 reps per set, 60 - second rest between sets, and a 90 - second rest after the 4th set; then 4 sets of 50% 1RM squat exercises, 6 - 8 reps per set, 60 - second rest between sets, and a 150 - second rest after the 4th set; 70% 1RM for bench press, 4 sets, 6 - 8 reps per set, 60 - second rest between sets, and a 90 - second rest after the 4th set; finally, 4 sets of 50% 1RM bench press exercises, 6 - 8 reps per set, 60 - second rest between sets. The total exercise time of the acute resistance training was about 30 min. The subjects were required to complete each movement with the joint range of motion to the full range.

[0108] Nutritional product intake and training lasted for 4 weeks. Two days of rest were taken after the last training session, and body composition, squat and bench press 1RM tests, and CMJ tests were carried out.

[0109] 1.3.2 Test indicators

[0110] Test serum insulin, EAA, and leucine contents.

[0111] Determine L - [cyclo -13 The enrichment of phenylalanine in C6 was measured to calculate the fractional synthesis rate of muscle protein.

[0112] Before and after the experimental intervention, the body morphological indexes of the subjects were measured: height, weight, and body composition.

[0113] Muscle strength indexes: 1RM tests of squats and bench presses, and CMJ test.

[0114] 1.4 Statistical analysis

[0115] All measured data were expressed as mean ± standard deviation (X±SD), and statistical analysis was performed using SPSS 25.0 software. One-way ANOVA was used for each index between groups and within groups, and P<0.05 indicated a significant difference.

[0116] 2 Experimental results

[0117] 2.1 Effects of ingesting whey protein composition on serum leucine, essential amino acids, and insulin

[0118] Figures 1 - 3 Tables 2 - 4 show the experimental results of the effects of ingesting the composition for improving or promoting whey protein absorption according to the present invention and the control group food on the contents of serum leucine, essential amino acids, and insulin. It can be seen from the figures that after ingesting the nutritional products, the serum leucine, essential amino acids, and insulin levels of each subject increased rapidly, and the increase rate in group D was faster than that in other groups. At the 30th minute and the 60th minute, the serum leucine, essential amino acids, and insulin levels in group D were significantly higher than those in groups A, B, C, E, and F (P<0.05).

[0119] Table 2: Effects of single supplementation of the composition for improving or promoting whey protein absorption on serum leucine

[0120]

[0121]

[0122] Table 3: Effects of single supplementation of the composition for improving or promoting whey protein absorption on essential amino acids

[0123]

[0124] Table 4: Effects of single supplementation of the composition for improving or promoting whey protein absorption on insulin

[0125]

[0126] Note: In Tables 2 to 4, * indicates a significant difference compared with Group A (P<0.05); # indicates a significant difference compared with Group B (P<0.05), & indicates a significant difference compared with Group C (P<0.05), ￥ indicates a significant difference compared with Group E (P<0.05), and @ indicates a significant difference compared with Group F (P<0.05).

[0127] 2.2 Effect of ingesting whey protein composition on fractional synthesis rate of muscle protein

[0128] The fractional synthesis rate of muscle protein (FSR) can represent the ability of protein to stimulate muscle protein synthesis after a meal. As shown in Table 5 and Figure 4 as indicated, the FSR of Group D was significantly higher than that of Group A, Group B, Group C, Group E, and Group F (P<0.05).

[0129] Table 5: Effect of single supplementation of composition for improving or promoting whey protein absorption on fractional synthesis rate of muscle protein

[0130] Group A B C D E F <![CDATA[FSR(%·h -1 )]]> 0.14±0.01 0.15±0.02 0.16±0.01 0.20±0.01*#&￥@ 0.17±0.02 0.17±0.01

[0131] 2.3 Effect of 4-week experimental intervention on body composition of subjects

[0132] As shown in Table 6, after 4-week experimental intervention, the body weight and lean body mass of the 4 groups of subjects increased. The increase in body weight and lean body mass of Group D subjects was significantly higher than that of Group A, Group B, Group C, Group E, and Group F (P<0.05).

[0133] Table 6: Effect of 4-week experimental intervention on body composition

[0134]

[0135]

[0136] 2.4 Effect of 4-week experimental intervention on 1RM of squat and bench press and CMJ vertical jump of subjects

[0137] As shown in Tables 7 and 8, 4-week experimental intervention increased the 1RM of squat and bench press and vertical jump height of the subjects. The increase in 1RM and vertical jump height of Group D subjects was significantly higher than that of Group A, Group B, Group C, Group E, and Group F (P<0.05).

[0138] Table 7: Effect of 4-week experimental intervention on 1RM of bench press and squat

[0139]

[0140] Table 8: Effect of 4-week experimental intervention on maximum vertical jump height

[0141] Grouping Before experimental intervention (cm) After 4 - week experimental intervention (cm) Δ (cm) Group A 37.2±7.9 40.4±7.7 3.2±0.7 Group B 37.4±6.7 41.2±7.3 3.8±1.3 Group C 37.9±8.2 41.5±10.8 3.6±1.3 Group D 36.9±5.3 42.3±6.9 <![CDATA[5.4±1.5 *#&￥@ > Group E 38.1±9.2 41.9±7.1 3.9±1.5 Group F 37.6±7.5 41.7±8.2 4.1±1.6

[0142] As described above, it is only the specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention can easily think of changes or substitutions, which should all be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention should be subject to the protection scope of the claims.

Claims

1. Use of a composition in the preparation of a product for improving or promoting the absorption of whey protein and increasing the muscle protein synthesis rate, wherein the composition consists of the following raw materials in parts by weight: 30 to 98 parts of whey protein, 0.001 to 0.06 parts of vitamin D3, and 0.002 to 2 parts of black pepper extract; The method for preparing the black pepper extract comprises the following steps: Crush black pepper, reflux and extract it 2 times with 95% ethanol which is 6 times the weight of black pepper for 1.5 hours each time, filter, after combining the filtrates, vacuum concentrate the filtrates to a paste to form a crude paste extract, freeze-dry the crude paste extract at -45°C, and then crush it with a ball mill to obtain a powder of the crude paste extract; Wash the powder of the crude paste extract 2 times at 25 to 60°C with a n-hexane washing solution, dissolve it with 95% ethanol which is 4 times the weight of the black pepper extract, filter with activated carbon, and vacuum concentrate to obtain a concentrated filtrate; Freeze-dry the concentrated filtrate obtained by vacuum concentration again at -15°C to form a freeze-dried product, and grind the freeze-dried product at -15°C to obtain black pepper extract particles with an average particle size of 15 to 30 μm.

2. The application according to claim 1, wherein The composition is composed of the following raw materials in parts by weight: 60 to 96 parts of whey protein, 0.004 to 0.02 parts of vitamin D3, and 0.006 to 1 part of black pepper extract.

3. The application according to claim 1, wherein The composition is composed of the following raw materials in parts by weight: 80 to 96 parts of whey protein, 0.002 to 0.013 parts of vitamin D3, and 0.01 to 0.5 part of black pepper extract.

4. The application according to any one of claims 1 to 3, wherein The whey protein comprises concentrated whey protein, separated whey protein and / or hydrolyzed whey protein.

5. A preparation, wherein The preparation comprises the composition for improving or promoting the absorption of whey protein and a pharmaceutically acceptable carrier or excipient; The composition is composed of the following raw materials in parts by weight: 30 to 98 parts of whey protein, 0.001 to 0.06 parts of vitamin D3, and 0.002 to 2 parts of black pepper extract; The method for preparing the black pepper extract comprises the following steps: Crush black pepper, reflux and extract it 2 times with 95% ethanol which is 6 times the weight of black pepper for 1.5 hours each time, filter, after combining the filtrates, vacuum concentrate the filtrates to a paste to form a crude paste extract, freeze-dry the crude paste extract at -45°C, and then crush it with a ball mill to obtain a powder of the crude paste extract; Wash the powder of the crude paste extract 2 times at 25 to 60°C with a n-hexane washing solution, dissolve it with 95% ethanol which is 4 times the weight of the black pepper extract, filter with activated carbon, and vacuum concentrate to obtain a concentrated filtrate; Freeze-dry the concentrated filtrate obtained by vacuum concentration again at -15°C to form a freeze-dried product, and grind the freeze-dried product at -15°C to obtain black pepper extract particles with an average particle size of 15 to 30 μm.

6. The preparation according to claim 5, wherein The preparation comprises 100 parts by weight of the composition and 0.01 to 3500 parts by weight of a pharmaceutically acceptable carrier or excipient.

7. The preparation according to claim 5, wherein The pharmaceutically acceptable carrier or excipient is selected from one or more of xylitol, erythritol, sorbitol, isomaltitol, isomaltose, maltodextrin, granulated sugar, ethanol, microcrystalline cellulose, lactose, maltodextrin, starch, hydroxypropyl methylcellulose, sodium carboxymethylcellulose, magnesium stearate and edible flavor.

8. The preparation according to claim 5, wherein the preparation is in the form of tablets, capsules, powders or granules.

9. Use of the preparation according to any one of claims 5 to 8 in the preparation of a muscle-building sports nutrition product.

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