Reagent tubes, multi-pak reagent tubes, liquid transfer devices, and methods of use thereof

Abstract

The embodiments of the present disclosure disclose a reagent tube, a multi-reagent tube, a liquid transfer device and a use method thereof. The reagent tube comprises a tube body having a first end and a second end, wherein the second end is a closed end; a first sealing plug configured to seal the first end, the first sealing plug having a first channel; the tube body, the first sealing plug and the closed second end collectively constitute a containing space, and under the action of an external force, the volume of the containing space is variable. The reagent tube of the present disclosure can change the volume of the containing space by means of external force, facilitate the outflow of liquid in the reagent tube, and is simple to operate; moreover, the multi-reagent tube and the liquid transfer device of the present disclosure can enable the transfer of liquid samples to be carried out in a fully closed state, enabling detection in a non-negative pressure environment such as a family or community, avoiding the risk of cross infection caused by aerosol; and the multi-channel integrated detection structure has high automation.

Description

Technical Field

[0001] The present disclosure relates to the technical field of biomedical instruments, and in particular to a reagent tube, a multiple reagent tube, a liquid transfer device, and a method of using the same. Background Art

[0002] In the production, testing or detection processes in the fields of biology, chemistry, food, medicine, epidemic prevention or environmental monitoring, the transfer of gaseous or liquid reagents is usually involved. Some liquid chemical reagents, such as ether, phenol and other volatile and toxic drugs; or some microbial pathogens that are prone to cause infections, allergies, tumors and other diseases, such as viruses, bacteria, rickettsia, mycoplasma, chlamydia, spirochetes, fungi, actinomycetes, etc., are mostly transferred using open suction and transfer devices, or pouring between reagent bottles. In this way, closed operations cannot be achieved during the reagent transfer process, which easily causes volatile toxic reagents to evaporate and diffuse outside the container, polluting the external environment and endangering the personal safety of the operator.

[0003] Diseases such as SARS, highly pathogenic avian influenza, and the novel coronavirus that have emerged in recent years are highly contagious and often cause global pandemics. Therefore, the transfer and detection of pathogens must be safe, rapid, and accurate. For example, nucleic acid testing, with its high sensitivity and good specificity, has important applications in disease diagnosis, epidemic prevention and control, and health monitoring. Current nucleic acid testing technologies primarily include the following two types of testing:

[0004] The first is traditional manual testing, which involves manually adding various biochemical reagents to PCR reaction tubes and transferring samples through a pipette. This method requires negative pressure and relies on the manual work of professional testers. Furthermore, sample storage, extraction, or transfer typically utilizes rigid reagent tubes made of materials such as glass, making sample extraction and transfer inconvenient. Furthermore, the process is complex and not highly automated, and cross-contamination of samples during testing or transfer can easily occur, leading to false positive results and other issues. Furthermore, the open environment presents an increased risk of infection for testers.

[0005] The second type is automated testing equipment. Currently, most automated nucleic acid testing equipment on the market uses independent processes for nucleic acid extraction, amplification, and detection. Each step requires a separate device, and a single nucleic acid test requires multiple devices. This requires significant space, and the sample needs to be transferred from one device to another after completing the previous step. This is cumbersome and time-consuming, and the sample transfer process is also susceptible to contamination from the external environment or the testing environment.

[0006] In addition, commercial nucleic acid detection equipment has also gradually appeared, including fully automatic nucleic acid detection equipment that integrates extraction, amplification and detection. However, most of them use single-channel or single-sample extraction and detection methods, that is, the detection equipment can only extract one sample at a time to detect a single pathogen, such as Cepheid's GeneXpert and bioMérieux's FilmArray. The detection throughput and efficiency of the above products are relatively low. Summary of the Invention

[0007] In order to solve the problems in the related art, the embodiments of the present disclosure provide a reagent tube, a multiple reagent tube, a liquid transfer device and a method of using the same.

[0008] In a first aspect, a reagent tube is provided in an embodiment of the present disclosure.

[0009] Specifically, the reagent tube includes:

[0010] a tube body having a first end and a second end, wherein the second end is configured as a closed end;

[0011] a first sealing plug configured to seal the first end, wherein the first sealing plug has a first channel;

[0012] The tube body, the first sealing plug and the closed second end together form an accommodating space, and under the action of an external force, the volume of the accommodating space is variable.

[0013] In combination with the first aspect, in a first implementation of the first aspect of the present disclosure, the bottom of the tube body forms the closed second end;

[0014] Alternatively, the reagent tube further includes: a second sealing plug configured to seal the second end.

[0015] In combination with the first implementation method of the first aspect, in the second implementation method of the first aspect of the present disclosure, the reagent tube also includes a supporting conduit located in the accommodating space, one end of the supporting conduit is inserted into at least a portion of the first channel, and the end of the other end has a first preset distance from the bottom of the tube body.

[0016] In combination with the first implementation of the first aspect, in a third implementation of the first aspect of the present disclosure, the second sealing plug has a liquid inlet channel and a liquid outlet channel connected to the liquid inlet channel;

[0017] The reagent tube further includes a supporting conduit located in the accommodating space, one end of the supporting conduit being inserted into at least a portion of the first channel, and the other end being in communication with the liquid outlet channel.

[0018] In combination with the third implementation method of the first aspect, in the fourth implementation method of the first aspect of the present disclosure, the other end of the supporting conduit is inserted into the liquid outlet channel, and a guide hole is opened on the tube wall of the supporting conduit, and the supporting conduit is connected to the liquid inlet channel through the guide hole.

[0019] In combination with the first aspect and the first to fourth implementations of the first aspect, in a fifth implementation of the first aspect of the present disclosure, the tube body is a flexible bag body.

[0020] In combination with the first to fourth implementations of the first aspect, in the sixth implementation of the first aspect of the present disclosure, the first sealing plug includes a first support column and a first frustum body, and the second sealing plug includes a second support column and a second frustum body, wherein the first support column and the first frustum body are formed by an integral or split molding method, and the second support column and the second frustum body are formed by an integral or split molding method.

[0021] In combination with the first aspect and the first to fourth implementations of the first aspect, in a seventh implementation of the first aspect of the present disclosure, a first fluid outlet is formed at the top of the first sealing plug, and the first fluid outlet is connected to the first channel.

[0022] In combination with the seventh implementation of the first aspect, the present disclosure, in an eighth implementation of the first aspect, further includes an elastic diaphragm covering the top surface of the first sealing plug, and the elastic diaphragm is configured to control the closing or opening of the first fluid outlet.

[0023] In combination with the eighth implementation manner of the first aspect, in a ninth implementation manner of the first aspect of the present disclosure, when in the closed state, the elastic diaphragm is in close contact with the first fluid outlet under the action of positive air pressure;

[0024] When in the open state, a liquid flow path is formed between the elastic diaphragm and the top surface of the first sealing plug under the action of negative air pressure.

[0025] In a second aspect, a multiple reagent tube is provided in an embodiment of the present disclosure.

[0026] Specifically, the multiple reagent tube includes:

[0027] Two or more reagent tubes in combination with the first aspect and the first to sixth implementations of the first aspect;

[0028] a fluid circuit valve strip configured to install and fix the reagent tubes, wherein the fluid circuit valve strip is provided with at least one transfer channel for transferring liquids between the reagent tubes;

[0029] The elastic valve membrane covers at least a portion of the fluid circuit valve strip.

[0030] In combination with the second aspect, in a first implementation of the second aspect of the present disclosure, two or more reagent tubes are fixed in the liquid circuit valve strip at predetermined intervals, wherein:

[0031] The top of the reagent tube is provided with a second fluid outlet, and the second fluid outlet is communicated with the first channel;

[0032] The elastic valve membrane is configured to control the closing or opening of the second fluid outlet.

[0033] In combination with the first implementation of the second aspect, in the second implementation of the second aspect of the present disclosure, when in an open state, the elastic valve membrane forms a liquid flow path with the top of the reagent tube under the action of negative air pressure, and the liquid flow path is connected to the transfer channel, so that the liquid in at least one reagent tube is transferred to another reagent tube via at least one transfer channel.

[0034] In a third aspect, a liquid transfer device is provided in an embodiment of the present disclosure.

[0035] Specifically, the device includes:

[0036] a driving unit configured to provide a power source;

[0037] A cartridge body having at least one channel, wherein each of the channels comprises at least a plurality of reagent tubes in combination with the first aspect and the first to sixth implementations of the first aspect;

[0038] The accommodating cavity is located below the card box body, and the accommodating cavity includes a plurality of hollow cavities corresponding to the card box body, and is used to accommodate and hold the plurality of reagent tubes.

[0039] In combination with the third aspect, in a first implementation of the third aspect of the present disclosure, the driving unit includes a control unit, a pneumatic valve plate and a plurality of pneumatic valves, the pneumatic valves are located at the bottom of the pneumatic valve plate, and the opening and closing of the pneumatic valves are controlled by the control unit.

[0040] In combination with the third aspect and the first implementation of the third aspect, in the second implementation of the third aspect of the present disclosure, the cartridge body further includes a liquid circuit valve plate, wherein

[0041] A plurality of the reagent tubes are fixed on the liquid circuit valve plate, and a third fluid outlet is formed at the top of the reagent tube;

[0042] The liquid path valve plate is provided with a first flow path main channel, and a first sub-channel selectively conductive between the first flow path main channel and the third fluid outlet.

[0043] With reference to the second implementation manner of the third aspect, in a third implementation manner of the third aspect, the liquid path valve plate is further provided with a second flow path main channel, and a second sub-channel selectively conductive between the second flow path main channel and the third fluid outlet of at least part of the reagent tube.

[0044] With reference to the third implementation manner of the third aspect, in a fourth implementation manner of the third aspect, a top surface of the reagent tube forms at least part of the first sub-channel and / or the second sub-channel.

[0045] With reference to the third implementation manner of the third aspect, the fourth implementation manner of the third aspect, in a fifth implementation manner of the third aspect, the cartridge body further comprises a first valve film, which is laid on a top of the liquid path valve plate, wherein the first valve film covers at least the first flow path main channel, the second flow path main channel, the first sub-channel, the second sub-channel, and the third fluid outlet of the reagent tube.

[0046] With reference to the fifth implementation manner of the third aspect, in a sixth implementation manner of the third aspect, the first valve film is laid in an integral and / or separate form.

[0047] With reference to the sixth implementation manner of the third aspect, in a seventh implementation manner of the third aspect, the pneumatic valve at least comprises a first pneumatic valve, which constitutes a first pneumatic valve film switch with at least part of the first valve film, and is configured to selectively conduct the first sub-channel and / or the second sub-channel.

[0048] With reference to the third aspect, the first implementation manner of the third aspect, the third implementation manner of the third aspect, the fourth implementation manner of the third aspect, the sixth implementation manner of the third aspect, or the seventh implementation manner of the third aspect, in an eighth implementation manner of the third aspect, the plurality of reagent tubes of each channel at least comprises a mixing pool tube, and the liquid path valve plate is provided with a pipetting channel selectively conductive with a fluid outlet of the mixing pool tube.

[0049] With reference to the eighth implementation manner of the third aspect, in a ninth implementation manner of the third aspect, the pneumatic valve further comprises a second pneumatic valve configured to conduct or close the fluid outlet of the mixing pool tube and the pipetting channel.

[0050] With reference to the eighth implementation manner of the third aspect, in a tenth implementation manner of the third aspect, the liquid path valve plate further comprises a dosing pool.

[0051] In combination with the tenth implementation manner of the third aspect, in the eleventh implementation manner of the third aspect of the present disclosure, a switch assembly is further provided on the liquid circuit valve plate, which is configured to selectively connect the quantitative pool with the pipetting channel or the second flow path main channel.

[0052] In combination with the eleventh implementation manner of the third aspect, in a twelfth implementation manner of the third aspect of the present disclosure, the pneumatic valve further includes a second pneumatic valve configured to selectively control the opening or closing of the switch assembly.

[0053] In combination with the eleventh implementation manner of the third aspect, in the thirteenth implementation manner of the third aspect of the present disclosure, the card box body also includes at least one PCR reagent tube, which is fixed to one side of the bottom of the liquid circuit valve plate, and the PCR reagent tube is connected to the quantitative pool.

[0054] In combination with the tenth to thirteenth implementations of the third aspect, in the fourteenth implementation of the third aspect of the present disclosure, the multiple reagent tubes of each channel also include at least one or more of a sample tube to be tested, a lysis liquid tube, a cleaning liquid tube, a waste liquid tube, a mineral oil tube, an eluent tube, and a spare reagent tube.

[0055] In combination with the third aspect, the first implementation manner of the third aspect, the third implementation manner, the fourth implementation manner, the sixth implementation manner, the seventh implementation manner, and the ninth to thirteenth implementation manners, in a fifteenth implementation manner of the third aspect of the present disclosure, the plurality of hollow cavities are respectively arranged at predetermined intervals along the first direction and the second direction of the accommodating cavity;

[0056] The plurality of hollow cavities arranged along the first direction are connected to each other through a plurality of first through holes;

[0057] In addition, a plurality of connection holes are opened on one side wall of the accommodating cavity, which are configured to be connected to the first power source, and the connection holes are communicated with the first through hole.

[0058] In combination with the fifteenth implementation method of the third aspect, in the sixteenth implementation method of the third aspect of the present disclosure, part of the hollow cavity is connected through a second through hole, and the accommodating cavity also includes a mixing chamber hole, which is configured to connect to a second power source, and the mixing chamber hole is connected to the second through hole.

[0059] In combination with the sixteenth implementation manner of the third aspect, in the seventeenth implementation manner of the third aspect of the present disclosure, the first power source and the second power source are air sources that provide positive and negative air pressures.

[0060] In combination with the first implementation, the third implementation, the fourth implementation, the sixth implementation, the seventh implementation, and the ninth to the thirteenth implementations of the third aspect, in the eighteenth implementation of the third aspect of the present disclosure, the control unit includes a solenoid valve.

[0061] In a fourth aspect, an embodiment of the present disclosure provides a method for using a liquid transfer device in combination with the third aspect and the first to eighteenth implementations of the third aspect.

[0062] The method for using the liquid transfer device comprises the following steps:

[0063] Pre-packaging the liquid reagents to be transferred in the reagent tubes of the cartridge body respectively;

[0064] The reagent tube is placed in the accommodating cavity, and the driving unit moves downward to press the cartridge body and the accommodating cavity tightly;

[0065] Under the action of the power source, the reagent tube is deformed, driving the liquid reagent in the reagent tube to transfer to other reagent tubes of the cartridge body through the first flow path main channel, thereby mixing with other reagents to form a reagent mixture.

[0066] In combination with the fourth aspect, in a first implementation of the fourth aspect of the present disclosure, the method further includes: deforming the reagent tube encapsulating the reagent mixture through the power source, thereby driving the reagent mixture to be transferred into the PCR reagent tube.

[0067] According to a technical solution provided by an embodiment of the present disclosure, a reagent tube includes: a tube body having a first end and a second end, wherein the second end is configured as a closed end; a first sealing plug configured to seal the first end, wherein the first sealing plug has a first channel; the tube body, the first sealing plug, and the closed second end together constitute a accommodating space, and the volume of the accommodating space is variable under the action of an external force. The reagent tube of the present disclosure can use an external force to change the volume of the accommodating space, thereby facilitating the outflow of the reagent in the reagent tube and avoiding the use of methods such as tipping the reagent tube, thereby making operation simpler.

[0068] According to another embodiment of the present disclosure, a multi-reagent tube comprises two or more reagent tubes, each of which has a variable volume under the action of an external force; a fluidic valve strip configured to mount and secure the reagent tubes, wherein the fluidic valve strip is provided with at least one transfer channel for transferring liquid between the reagent tubes; and an elastic valve membrane covering at least a portion of the fluidic valve strip. The multi-reagent tube of the present disclosure allows for contactless transfer of reagents in a fully enclosed state, avoiding the risk of cross-infection caused by factors such as the external environment.

[0069] According to another embodiment of the present disclosure, a technical solution is provided, a liquid transfer device, comprising: a drive unit configured to provide a power source; a cartridge body having at least one channel, and each channel is independent of each other, wherein each channel includes at least a plurality of reagent tubes, and the volume of the reagent tubes is variable under the action of an external force; a receiving cavity located below the cartridge body, the receiving cavity including a plurality of hollow cavities corresponding to the cartridge body, for accommodating and holding the plurality of reagent tubes. During liquid transfer, the drive unit moves downward to press the cartridge body to hold the reagent tubes in the hollow cavities to form an overall sealed structure. The liquid transfer process of the present disclosure is carried out in a fully enclosed state, and liquid reagents can be transferred and tested in a non-negative pressure biological experimental environment, avoiding the risk of cross infection caused by aerosols; and the multi-channel integrated liquid transfer and detection structure can perform liquid transfer and detection separately in each channel, and can also realize simultaneous transfer and detection of different individual samples in multiple channels, with a high degree of automation and improved liquid transfer and detection throughput and liquid transfer and detection efficiency.

[0070] It is to be understood that the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the disclosure. BRIEF DESCRIPTION OF THE DRAWINGS

[0071] Other features, objectives and advantages of the present disclosure will become more apparent through the following detailed description of non-limiting embodiments in conjunction with the accompanying drawings. In the accompanying drawings:

[0072] Figure 1 A schematic structural diagram of a reagent tube according to an embodiment of the present disclosure is shown;

[0073] Figure 2 A top view of a reagent tube according to an embodiment of the present disclosure is shown;

[0074] Figure 3 FIG2 shows a schematic cross-sectional structure diagram of a reagent tube taken along section line CC according to an embodiment of the present disclosure;

[0075] Figure 4 An enlarged view of a partial structure of a second sealing plug of a reagent tube according to an embodiment of the present disclosure is shown;

[0076] Figure 5 A schematic structural diagram of a multiple reagent tube according to another embodiment of the present disclosure is shown;

[0077] Figure 6 FIG2 shows a top view of a fluid circuit valve strip of a multiple reagent tube according to another embodiment of the present disclosure;

[0078] Figure 7 FIG2 shows a schematic structural diagram of a multi-channel liquid transfer device according to another embodiment of the present disclosure;

[0079] Figure 8 A schematic structural diagram of a multi-channel cartridge body according to another embodiment of the present disclosure is shown;

[0080] Figure 9 A front view of a multi-channel cartridge body according to another embodiment of the present disclosure is shown;

[0081] Figure 10 A perspective view showing a fluid circuit valve plate according to yet another embodiment of the present disclosure;

[0082] Figure 11 An enlarged view of a partial structure of a liquid circuit valve plate according to another embodiment of the present disclosure is shown;

[0083] Figure 12 A top view of a receiving cavity according to another embodiment of the present disclosure is shown;

[0084] Figure 13 A cross-sectional view of an accommodating cavity according to another embodiment of the present disclosure is shown along the AA section line;

[0085] Figure 14 A bottom view showing a driving portion according to yet another embodiment of the present disclosure;

[0086] Figure 15 An enlarged view of a partial structure of a pneumatic valve according to another embodiment of the present disclosure is shown;

[0087] Figure 16 A flow chart showing a method for using a multi-channel liquid transfer device according to yet another embodiment of the present disclosure is shown.

[0088] Among them, the specific drawings are marked as follows:

[0089] 100-reagent tube;

[0090] 101-Tube body;

[0091] 102 - first sealing plug; 1021 - first channel; 1022 - first sealing plug fluid outlet; 1023 - first support column; 1024 - first frustum body;

[0092] 103 - second sealing plug; 1031 - liquid inlet channel; 1032 - liquid outlet channel; 1033 - second support column; 1034 - second frustum body;

[0093] 104 - accommodating space; 105 - supporting conduit; 1051 - guide hole;

[0094] 106-elastic diaphragm;

[0095] 200-multiple reagent tubes;

[0096] 100′-reagent tube;

[0097] 101′-tube body;

[0098] 102′-first sealing plug; 1021′-first channel; 1022′-first sealing plug fluid outlet; 1023′-first support column; 1024′-first frustum body;

[0099] 103′- second sealing plug; 1033′- second supporting column; 1034′- second frustum body;

[0100] 104′-accommodation space; 105′-support conduit; 1051′-guide hole;

[0101] 202-Liquid valve strip; 2021-Transfer channel;

[0102] 203-elastic valve membrane;

[0103] 300-multi-channel sample liquid transfer device;

[0104] 301-driving unit; 3011-control unit; 3012-pneumatic valve plate; 3013-pneumatic valve; 30131-first pneumatic valve; 30132-second pneumatic valve;

[0105] 302-card box body;

[0106] 3021 - reagent tube; H - mixing tank tube; X - eluent tube; K - mineral oil tube; Q - cleaning liquid tube; F - waste liquid tube; B - spare reagent tube; L - lysis solution tube; Y - sample tube to be tested; 30211 - fluid outlet; 3026 - PCR reagent tube;

[0107] 3022 - multi-channel fluid path valve plate; 30221 - first fluid path main channel; 30222 - first sub-channel; 30223 - second fluid path main channel; 30224 - second sub-channel; 30225 - pipetting channel; 30226 - quantitative reservoir; A - first switch; C - second switch;

[0108] 3023-first valve membrane;

[0109] 303 - accommodating cavity; hollow cavity 3031; 3032 - first through hole; 3033 - connecting hole; 3034 - second through hole; mixing cavity hole - 3035.

[0110] It should be understood that the size of the various components shown in the accompanying drawings is not drawn according to actual proportional relationships. In addition, the same or similar reference numerals represent the same or similar components. DETAILED DESCRIPTION

[0111] Hereinafter, exemplary embodiments of the present disclosure will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily implement them. In addition, for the sake of clarity, parts not related to the description of the exemplary embodiments are omitted in the accompanying drawings.

[0112] In the present disclosure, it should be understood that terms such as "include" or "have" are intended to indicate the presence of features, numbers, steps, actions, components, parts, or combinations thereof disclosed in the present specification, and are not intended to exclude the possibility that one or more other features, numbers, steps, actions, components, parts, or combinations thereof exist or are added.

[0113] It should also be noted that, in the absence of conflict, the embodiments and features of the embodiments of the present disclosure may be combined with each other. The present disclosure will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0114] As mentioned above, when storing, extracting or transferring pathogen samples or reaction reagents, rigid reagent tubes, such as glass reagent tubes, are often used. When samples need to be extracted or transferred, or when transferring samples from a reagent tube to another reagent tube, manual operation is required to aspirate or pour the sample from one reagent tube into another reagent tube. This process places extremely high demands on the operator, and the process is complex with a low degree of automation. In addition, cross-contamination is prone to occur during the extraction or transfer process, and the probability of testers being infected with the virus is easily increased in an open environment.

[0115] In order to solve the above-mentioned defects, an embodiment of the present disclosure provides a reagent tube. The reagent tube includes: a tube body, having a first end and a second end, wherein the second end is configured as a closed end; a first sealing plug, configured to seal the first end, wherein the first sealing plug has a first channel; the tube body, the first sealing plug and the closed second end together constitute a accommodating space, and under the action of an external force, the volume of the accommodating space is variable. When transferring or extracting a sample, it is only necessary to use an external force to change the volume of the accommodating space of the tube body, so that the sample in the reagent tube can be squeezed out of the reagent tube, realizing automatic transfer or extraction of the sample. This process can be achieved through automated equipment, which is simple and efficient, and avoids manual operation. In addition, the fluid outlet of the reagent tube can be connected to the pipetting channel to realize contactless transfer of the sample to be tested, reducing the possibility of cross contamination.

[0116] Figure 1 A schematic structural diagram of a reagent tube according to an embodiment of the present disclosure is shown. Figure 2 A top view of a reagent tube according to an embodiment of the present disclosure is shown. Figure 3 A schematic diagram of the cross-sectional structure of a reagent tube taken along the CC section line according to an embodiment of the present disclosure is shown. Figure 4A partial structure enlarged view of a second sealing plug of a reagent tube according to an embodiment of the present disclosure is shown.

[0117] As shown in Figures 1 to 4 , a reagent tube 100 comprises:

[0118] a tube body 101 having a first end and a second end, wherein the second end is configured as a closed end;

[0119] a first sealing plug 102 configured to seal the first end, wherein the first sealing plug 102 has a first channel 1021, and a top of the first sealing plug 102 further forms a fluid outlet 1022 which is in communication with the first channel 1021;

[0120] the tube body 101, the first sealing plug 102 and the closed second end jointly constitute a containing space 104, and under the action of an external force, the tube body 101 can be deformed by extrusion, so that the volume of the containing space 104 changes, thereby extruding the liquid or reaction reagent stored in the containing space 104 out of the fluid outlet 1022 through the first channel 1021.

[0121] wherein the second end of the tube body 101 can be a bottom closed structure integrally formed with the tube body 101, i.e., the bottom of the tube body 101 is closed to form the second end; in addition, as a preferred embodiment, the reagent tube can further comprise a second sealing plug 103 configured to seal the second end of the tube body 101. Figure 1

[0122] According to the reagent tube 100 of the present disclosure, a support conduit 105 is located in the containing space 104, one end of the support conduit 105 is inserted into the inside of the first channel 1021, and the other end has a first preset distance between the end and the bottom of the tube body 101. When the tube body 101 is deformed under the action of an external force, the liquid reagent in the containing space 104 can flow into the support conduit 105 and flow out through the first channel 1021.

[0123] The tube body 101 can be a flexible bag body, such as a tubular bag body made of PE, PVC film or TPU film. The external force can include mechanical force or force provided by a positive or negative air pressure source. The embodiments of the present disclosure do not particularly limit the external force, and any external force that can drive the flexible bag body to deform is acceptable.

[0124] According to the embodiments of the present disclosure, as shown in Figure 1 and 3 ​As shown, the second sealing plug 103 has a liquid inlet channel 1031 and a liquid outlet channel 1032 connected to the liquid inlet channel 1031; one end of the support conduit 105 is inserted into at least a portion of the first channel 1021, and the other end is connected to the liquid outlet channel 1032. Under the action of positive air pressure, the liquid to be transferred or the reaction reagent in the accommodating space 104 flows into the support conduit 105 through the liquid inlet channel 1031 of the second sealing plug 103, and finally flows out through the fluid outlet 1022. Preferably, as Figure 4 As shown, the other end of the support conduit 105 can be inserted into the liquid outlet channel 1032, and a guide hole 1051 is opened on the wall of the portion of the support conduit 105 inserted into the liquid outlet channel 1032 (see Figure 1 ), the aperture and position of the guide hole 1051 are adapted to the liquid inlet channel 1031, and the support conduit 105 is connected to the liquid inlet channel 1031 through the guide hole 1051. The liquid inlet channel 1031 disclosed in the present invention can be set to be multiple, for example, two, three, four or more liquid inlet channels 1031 that can be connected to the liquid outlet channel 1031 are arranged on the second sealing plug 103.

[0125] like Figure 1 As shown, the first sealing plug 102 includes a first support column 1023 and a first frustum body 1024, and the second sealing plug 103 includes a second support column 1033 and a second frustum body 1034. The first support column 1023 and the first frustum body 1024, as well as the second support column 1033 and the second frustum body 1034, can be formed in an integral or split molding manner. The embodiments of the present disclosure all adopt an integral molding manner to form the above structure, see Figure 3 .

[0126] In addition, according to the embodiment of the present disclosure, Figure 2 As shown, the reagent tube 100 further includes an elastic diaphragm 106, which covers the top surface of the first sealing plug 102. The elastic diaphragm 106 is configured to control the closing or opening of the fluid outlet 1022. When the elastic diaphragm 106 is in the closed state, the elastic diaphragm 106 is in close contact with the fluid outlet 1022 under the action of positive air pressure, so that the reagent tube 100 remains sealed. When the elastic diaphragm 106 is in the open state, the elastic diaphragm 106 is deformed and bulged under the action of negative air pressure. At this time, the elastic diaphragm 106 can form a liquid flow path between the elastic diaphragm 106 and the top surface of the first sealing plug 102, so that the liquid in the reagent tube 100 can flow out through the liquid flow path or achieve liquid transfer with other reagent tubes.

[0127] The reagent tube 100 of the embodiment of the present disclosure is easy to operate and has a high degree of automation. It can also achieve contactless transfer during the transfer of the liquid to be transferred or the reaction reagent, thus avoiding contamination from the external environment.

[0128] According to another embodiment of the present disclosure, a multiple reagent tube is provided, such as Figure 5 and 6 shown. Figure 5 A schematic structural diagram of a multiple reagent tube according to another embodiment of the present disclosure is shown. Figure 6 A top view of a multiple reagent tube according to another embodiment of the present disclosure is shown.

[0129] Reference Figure 5 and 6 , while combining Figure 1-4 As shown, an embodiment of the present disclosure provides a multiple reagent tube 200, which includes: two or more reagent tubes 100', and the reagent tubes 100' are fixed at a predetermined interval. The present disclosure is described in the form of two reagent tubes, but it should be noted that the multiple reagent tube of the present disclosure can include two or more reagent tubes;

[0130] Among them, such as Figure 5 As shown, the reagent tube 100 ′ includes a tube body 101 ′ having a first end and a second end, wherein the second end is configured as a closed end;

[0131] A first sealing plug 102 ′ is configured to seal the first end, wherein the first sealing plug 102 ′ has a first channel 1021 ′ and a fluid outlet 1022 ′ is formed at the top of the first sealing plug 102 ′, and the fluid outlet 1022 ′ is in communication with the first channel 1021 ′;

[0132] The tube body 101′, the first sealing plug 102′ and the closed second end together constitute the accommodating space 104′. Under the action of external force, the tube body 101′ can be squeezed and deformed, so that the volume of the accommodating space 104′ changes, thereby squeezing the liquid to be transferred or the reaction reagent stored in the accommodating space 104′ out from the fluid outlet 1022′ through the first channel 1021′.

[0133] The second end of the tube body 101′ can be a bottom-enclosed structure integrally formed with the tube body 101′, that is, the bottom of the tube body 101′ is enclosed to form the second end; in addition, as a preferred embodiment, the reagent tube 100′ can also include a second sealing plug 103′, which is configured to seal the second end of the tube body 101′. Figure 1 shown.

[0134] According to the reagent tube 100' of the embodiment of the present disclosure, it also includes a supporting conduit 105', which is located in the accommodating space 104'. One end of the supporting conduit 105 is inserted into the interior of the first channel 1021, and a first preset distance is formed between the end of the other end and the bottom of the tube body 101'. When the tube body 101' is deformed under the action of an external force, the liquid reagent in the accommodating space 104' can flow into the supporting conduit 105' and flow out through the first channel 1021'. Among them, the tube body 101' can be a flexible bag, such as a tubular reagent bag made of PE, PVC film, or TPU film. The external force can be provided by a positive and negative air pressure source.

[0135] The structure of the second sealing plug 103 ′ according to the embodiment of the present disclosure is identical to that of the first embodiment, and comprises a liquid inlet channel 1031 ′ and a liquid outlet channel 1032 ′ connected to the liquid inlet channel 1031 ′;

[0136] See also Figure 5 The reagent tube 100′ further includes a support conduit 105′, which is located in the accommodating space 104′. One end of the support conduit 105′ is inserted into at least a portion of the first channel 1021′, and the other end is connected to the liquid outlet channel. Under the action of positive air pressure, the liquid to be transferred or the reaction reagent in the accommodating space 104′ flows into the support conduit 105′ through the liquid inlet channel of the second sealing plug 103′, and finally flows out through the fluid outlet 1022′. The structure of the support conduit 105′ of this embodiment is exactly the same as that of the aforementioned embodiment 1. The other end of the support conduit 105′ can be inserted into the liquid outlet channel 1032′, and a guide hole 1051′ is opened on the wall of the portion of the support conduit 105′ inserted into the liquid outlet channel 1032′. The aperture of the guide hole 1051′ is adapted to the liquid inlet channel 1031′, and the support conduit 105′ is connected to the liquid inlet channel 1031′ through the guide hole 1051′.

[0137] like Figure 5 As shown, the first sealing plug 102 ′ includes a first support column 1023 ′ and a first frustum body 1024 ′, and the second sealing plug 103 ′ includes a second support column 1033 ′ and a second frustum body 1034 ′.

[0138] The multiple reagent tube 200 of the present disclosure further includes a fluid valve strip 202 configured to install and fix the reagent tubes 100 ′, wherein the fluid valve strip 202 is provided with at least one transfer channel 2021 for transferring liquid between the reagent tubes 100 ′;

[0139] The elastic valve membrane 203 covers the upper surface of the liquid valve strip 202 and can encapsulate the liquid valve strip 202. The elastic valve membrane 203 is configured to control the closing or opening of the fluid outlet 1022'. When the elastic valve membrane 203 is in the open state, it deforms and bulges under the action of negative pressure, forming a liquid flow path with the top of the reagent tube 100'. This liquid flow path can be connected to the transfer channel 2021, allowing the liquid in one reagent tube 100' to be transferred to the other reagent tube via the transfer channel 2021.

[0140] The multiple reagent tubes 200 of the disclosed embodiment form a fully enclosed liquid transfer structure, which is easy to operate and highly automated, and can achieve contactless transfer of liquid reagents to avoid contamination from the external environment.

[0141] As mentioned earlier, the traditional manual nucleic acid detection method has a complex operation process and a low degree of automation, and samples are prone to cross-contamination during testing or transfer. Existing automated detection equipment requires multiple devices to work together, and samples after the previous steps are completed need to be transferred to subsequent equipment. The operation is cumbersome and time-consuming, and the sample transfer process is also prone to contamination by the external environment or contamination of the detection environment, resulting in low transfer and detection throughput and detection efficiency.

[0142] According to another embodiment provided by the present disclosure, a liquid transfer device includes: a drive unit configured to provide a power source; a cartridge body having at least one channel, and each channel is independent of each other, wherein each channel includes at least a plurality of reagent tubes, and the volume of the reagent tubes is variable under the action of an external force; and a accommodating cavity located below the cartridge body, the accommodating cavity including a plurality of hollow cavities corresponding to the cartridge body, for accommodating and retaining the plurality of reagent tubes. When transferring liquid reagents, the drive unit moves downward to press the cartridge body to retain the reagent tubes in the hollow cavities to form an overall closed structure. The device disclosed herein can always ensure that it is in a fully closed state during liquid transfer, and there is no need for contact with atmospheric air due to excessive reactions. It can realize liquid transfer and detection in non-negative pressure biological experimental environments at home, in the community, and outdoors, and will not cause cross-infection caused by aerosols. Moreover, through the multi-channel integrated detection structure, liquid transfer and detection can be carried out separately in each channel, and simultaneous transfer and detection of different individual samples in multiple channels can be realized, which has a high degree of automation and improves the transfer and detection throughput and detection efficiency.

[0143] Figure 7 A schematic structural diagram of a multi-channel liquid transfer device according to another embodiment of the present disclosure is shown. Figure 8 A schematic structural diagram of a multi-channel cartridge body according to another embodiment of the present disclosure is shown. Figure 9A front view of a multi-channel cartridge body according to yet another embodiment of the present disclosure is shown. Figure 10 A perspective view illustrating a fluid circuit valve plate according to yet another embodiment of the present disclosure is shown. Figure 11 An enlarged view of the partial structure of a fluid circuit valve plate according to yet another embodiment of the present disclosure is shown. Figure 12 A top view of a receiving cavity according to another embodiment of the present disclosure is shown. Figure 13 A cross-sectional view of an accommodating cavity according to yet another embodiment of the present disclosure is shown along section line AA. Figure 14 A bottom view of a driving portion according to yet another embodiment of the present disclosure is shown. Figure 15 An enlarged view of a partial structure of a pneumatic valve according to yet another embodiment of the present disclosure is shown.

[0144] like Figure 7 As shown, the multi-channel liquid transfer device 300 includes:

[0145] The driving unit 301 is configured to provide a power source;

[0146] The cartridge body 302 has at least one channel. It will be understood by those skilled in the art that when the cartridge body 302 has one channel, the cartridge body 302 is a single-row structure; when the cartridge body 302 has two or more channels, the cartridge body is a multi-row channel structure, i.e., a multi-channel cartridge body 302 (e.g., Figure 7 As shown), and each channel is independent of each other, wherein each channel includes at least a plurality of reagent tubes 3021, and under the action of external force, the volume of the reagent tube 3021 is variable; the structure of the reagent tube 3021 of the embodiment of the present disclosure is different from that of the aforementioned embodiment (see Figure 1-4 ) compared to the reagent tube 100 of the embodiment, the reagent tube 3021 of this embodiment has the same structure as the reagent tube 100 of the aforementioned embodiment except that it does not include the elastic diaphragm 106. Therefore, the present disclosure will not further describe the structure of the reagent tube 3021. For the specific structure of the reagent tube 3021 of the embodiment of the present disclosure, please refer to Figures 1 to 4 shown.

[0147] The accommodating cavity 303 is located below the multi-channel cartridge body 302. The accommodating cavity 303 includes a plurality of hollow cavities 3031 corresponding to the multi-channel cartridge body 302, and is used to accommodate and hold a plurality of reagent tubes 3021. When liquid is transferred, the driving unit 301 moves downward to press the multi-channel cartridge body 302 to hold the plurality of reagent tubes 3021 in the hollow cavities 3031 to form an overall sealed structure. Figure 8 and 9As shown, the cartridge body 302 includes a multi-channel liquid circuit valve plate 3022. This disclosure uses eight channels as an example for illustration. It should be understood that the multi-channels disclosed herein may refer to two or more channels, and each channel may include multiple reagent tubes 3021, for example, eight. Those skilled in the art can design the number of channels and reagent tubes based on actual application needs, and this disclosure does not impose any particular restrictions on this. To clearly explain the technical solution of this disclosure, this disclosure uses an 8*8 matrix multi-channel cartridge body for nucleic acid extraction as an example for illustration.

[0148] The multiple reagent tubes 3021 include at least one or more of a mixing tank tube H, an eluent tube X, a mineral oil tube K, a cleaning liquid tube Q, a waste liquid tube F, a spare reagent tube B, a lysis liquid tube L, and a sample tube Y. This disclosure does not impose any particular limitation on this.

[0149] According to the embodiments of the present disclosure, Figure 10 and 11 As shown, 8*8 reagent tubes 3021 are fixedly mounted on the bottom of the eight-channel fluid circuit valve plate 3022. Each reagent tube 3021 can be fixed or detachably mounted, and a fluid outlet 30211 is formed at the top of each reagent tube 3021. Each channel of the eight-channel fluid circuit valve plate 3022 is provided with a first main flow channel 30221. A first sub-channel 30222 that is selectively connectable is formed between the first main flow channel 30221 and the fluid outlet 30211.

[0150] In addition, each channel of the liquid circuit valve plate 3022 is also provided with a second flow path main channel 30223, and a second sub-channel 30224 that can be selectively connected is formed between the second flow path main channel 30223 and the fluid outlet 30211 of at least part of the reagent tube 3021. Among them, at least part of the reagent tube 3021 includes: a mixing pool tube H, an eluent tube X, a mineral oil tube K, etc. The mixed liquid in the mixing pool tube H, the eluent in the eluent tube X, or the mineral oil in the mineral oil tube K can flow into the second flow path main channel 30223 via their respective second sub-channels 30244, and be transported to the corresponding reagent tube or quantitative pool as needed. The present disclosure does not make any special restrictions on this, and those skilled in the art can adaptively control and adjust according to the needs of transfer, extraction or detection.

[0151] According to an embodiment of the present disclosure, the top surfaces of the plurality of reagent tubes 3021 may form at least a portion of the first sub-channel 30222 and / or the second sub-channel 30224. For example, the top surfaces of the reagent tubes 3021 may be flat, curved, or form a channel structure.

[0152] like Figures 9 to 11As shown, the cartridge body 302 also includes a first valve membrane 3023, which can be laid on the top of the liquid circuit valve plate 3022 in an integral and / or split form, wherein the first valve membrane 3023 covers at least the first flow path main channel 30221, the second flow path main channel 30223, the first sub-channel 30222, the second sub-channel 30224 and the fluid outlet 30211 of the reagent tube 3021.

[0153] According to the embodiments of the present disclosure, Figure 7 and 14 As shown, the driving unit 301 includes a control unit 3011, a pneumatic valve plate 3012, and a plurality of pneumatic valves 3013. The pneumatic valves 3013 are located at the bottom of the pneumatic valve plate 3012, and the opening and closing of the pneumatic valves 3013 are controlled by the control unit 3011. Preferably, the control unit 3011 includes at least a solenoid valve.

[0154] The pneumatic valve 3013 at least includes a first pneumatic valve 30131 , which together with at least a portion of the first valve membrane 3023 constitutes a first pneumatic valve membrane switch, and is configured to selectively open the first sub-channel 30222 and / or the second sub-channel 30224 . For example, when the first pneumatic valve 30131 provides positive air pressure, the first valve membrane 3023 presses the fluid outlet 30211. At this time, the first pneumatic valve membrane switch is in a closed state, so that the fluid outlet 30211 remains in a closed state; when the first pneumatic valve 30131 provides negative air pressure, at least a portion of the first valve membrane 3023 is deformed and bulged under the action of negative pressure, and at least a portion of the first valve membrane 3023 can form a conductive first sub-channel 30222 and / or second sub-channel 30224 at the top of the reagent tube 3021. At this time, the first pneumatic valve membrane switch is in an open state, so that the liquid between the reagent tubes 3021 can be merged into the first flow path main channel 30221 or the second flow path main channel 30223 through the first sub-channel 30222 or the second sub-channel 30224, thereby facilitating the transfer of liquid.

[0155] According to the embodiments of the present disclosure, Figure 10 As shown, the liquid circuit valve plate 3022 is also provided with a pipetting channel 30225 that selectively communicates with the fluid outlet of the mixing pool tube H.

[0156] like Figure 10 As shown, the liquid circuit valve plate 3022 is also provided with a quantitative reservoir 30226 and a switch assembly, wherein the quantitative reservoir 30226 is configured to quantitatively transport the liquid to be transferred, and the switch assembly is configured to selectively connect the quantitative reservoir 30226 to the pipetting channel 30225 or the second flow path main channel 30223. Correspondingly, the pneumatic valve 3013 also includes a second pneumatic valve 30132, see Figure 14 , configured to selectively control the switch component to open or close.

[0157] Combine Figure 10 and 14 As shown, the switch assembly includes a first switch A and a second switch C, wherein the first switch A includes a horizontal switch and a vertical switch. When the vertical switch of the first switch A and the second switch C are closed and the horizontal switch of the first switch A is disconnected, the reagent mixture in the mixing pool tube H can be transferred to the quantitative pool 30226. After the quantitative pool 30226 is filled, the vertical switch of the first switch A is disconnected, and the horizontal switch of the first switch A is closed. At the same time, the second switch C is kept in a closed state. At this time, the mineral oil in the mineral oil tube is pushed into the quantitative pool 30266 to quantitatively transfer the reagent mixture in the quantitative pool 30266.

[0158] In addition, according to the embodiments of the present disclosure, Figure 9 As shown, at least one PCR reagent tube 3026 is also installed on the bottom side of the end of the cartridge body 302. The PCR reagent tube 3026 is located at the end of the flow path of each channel and is connected to the quantitative reservoir 30226 of the liquid circuit valve plate 3022. The reagent mixture in the quantitative reservoir 30226 can be quantitatively transported to the PCR reagent tube for fluorescent PCR amplification detection. Each channel of the multi-channel cartridge body of the disclosed embodiment includes at least a sample tube Y to be tested, a lysis solution tube L, a cleaning solution tube Q, a waste liquid tube F, a mineral oil tube K, an eluent tube X, a quantitative reservoir 30226, and a PCR reagent tube 3026.

[0159] According to the embodiment of the present disclosure, Figure 7 、 12 As shown in FIG13 , the plurality of hollow cavities 3031 in the accommodating cavity 303 of the present disclosure are respectively arranged along the first direction (ie, X direction) of the accommodating cavity 303. Figure 12 As shown in the figure, the reagent tubes 3021 in the multi-channel cartridge body 302 are evenly arranged in the first direction (i.e., the second direction) according to a predetermined spacing distance, and the layout thereof corresponds to that of the reagent tubes 3021 in the multi-channel cartridge body 302;

[0160] The plurality of hollow cavities 3031 arranged along the first direction are interconnected via a plurality of first through-holes 3032. Furthermore, a plurality of connection holes 3033 are provided on the sidewall of the accommodating cavity 303 along the first direction, configured to connect to a first power source. The plurality of connection holes 3033 are respectively connected to the plurality of first through-holes 3032. The first power source may be a positive or negative air pressure power source. When connected to the positive or negative air pressure power source, the flexible reagent tube 3021 retained in the hollow cavity 3031 can be deformed by selectively providing positive or negative air pressure, thereby causing the liquid to be transferred or the reaction reagent in the reagent tube 3021 to flow out of the fluid outlet 30211.

[0161] Preferably, if Figure 13As shown, some of the cavities in the hollow cavity 3031, such as the hollow cavity 3031 corresponding to the mixing pool tube H in the cartridge body 302, can be connected through a plurality of second through holes 3034, and a mixing cavity hole 3035 is further provided on the side wall of the accommodating cavity 303, and the mixing cavity hole 3035 is connected to the second through hole 3034 and is configured to be connected to the second power source. Wherein, the second power source can be a positive and negative air pressure power source. Under the alternating action of the positive and negative pressures of the external positive and negative air pressure power source, the liquid to be transferred and the reaction reagent in the mixing pool tube H can be fully mixed and uniformly mixed to ensure the accuracy of the test results.

[0162] Figure 16 A flow chart showing a method for using a multi-channel liquid transfer device according to another embodiment of the present disclosure is shown.

[0163] According to an embodiment of the present disclosure, a method for using a multi-channel liquid transfer device is provided, wherein the multi-channel liquid transfer device is the multi-channel liquid transfer device described in the aforementioned embodiment. The structure of the multi-channel liquid transfer device is not further described in this disclosure.

[0164] The method for using the multi-channel liquid transfer device comprises the following steps:

[0165] Pre-packaging the liquid reagents to be transferred in the reagent tubes of the multi-channel cartridge body;

[0166] Place the reagent tube in the accommodating cavity, and move the driving unit downward to press the cartridge body and the accommodating cavity tightly;

[0167] Under the action of the power source, the reagent tube is deformed, driving the liquid reagent in the reagent tube to be transferred to other reagent tubes of the cartridge body through the first flow path main channel, thereby mixing with other reagents to form a reagent mixture.

[0168] The present disclosure uses nucleic acid extraction and detection as an example to specifically illustrate the method of using the multi-channel liquid transfer device.

[0169] The method includes the following steps:

[0170] Pre-packaging multiple groups of samples to be tested (e.g., nucleic acid samples) and reagents required for sample extraction (e.g., mineral oil, eluent, lysate, and cleaning solution) in sample reagent tubes and multiple liquid storage reagent tubes of the multi-channel cartridge body, wherein the multiple liquid storage reagent tubes include one or more of a lysate tube, a cleaning solution tube, a waste liquid tube, a mineral oil tube, an eluate tube, and a spare reagent tube;

[0171] The sample reagent tube and multiple liquid storage reagent tubes are placed in a hollow cavity of a housing body that is adapted to the shape and number of the sample reagent tubes. The hollow cavity is a multi-channel structure in a rectangular array layout corresponding to the multi-channel cartridge body. The cavities in each column of the rectangular array of hollow cavities are connected to each other through a first through hole and are connected to an external positive and negative air pressure source through a connecting hole.

[0172] The driving unit moves downward under the control of the control unit to press the multi-channel cartridge body and the accommodating cavity tightly, so that the sample reagent tube and the multiple liquid storage reagent tubes are sealed and retained in the hollow cavity;

[0173] When extracting the nucleic acid sample to be tested, the sample reagent tube and multiple liquid storage reagent tubes located in the hollow cavity are deformed under the action of the positive and negative air pressure sources, driving the nucleic acid sample to be tested in the sample reagent tube and the reagents in each liquid storage reagent tube to be transferred through the first main flow channel to the mixing reagent tube for sufficient mixing and reaction;

[0174] In the detection step, the nucleic acid sample to be detected after the mixed reaction is quantitatively transferred to the PCR reagent tube through the quantitative pool under the action of positive and negative air pressure sources, and fluorescent PCR amplification detection is performed in the PCR reagent tube.

[0175] The liquid transfer device disclosed herein can always ensure that it is in a fully closed state during liquid transfer, and there is no need for contact with atmospheric air due to excessive reactions. It can realize transfer and detection in non-negative pressure biological experimental environments at home, in the community, and outdoors, and will not cause cross-infection caused by aerosols. Moreover, through the multi-channel integrated detection structure, it is possible to transfer and detect liquid samples separately in each channel, and it is also possible to realize simultaneous transfer and detection of different individual samples in multiple channels, with a high degree of automation, and improve the transfer and detection throughput as well as the transfer and detection efficiency.

[0176] In addition, it should be noted that the reagent tube disclosed in the present invention actually only includes one fluid outlet, and the expressions "first fluid outlet", "second fluid outlet" and "third fluid outlet" mentioned in the text are only for the sake of clarity, to facilitate the distinction between the fluid outlets of the reagent tubes appearing in different embodiments. Therefore, the "first fluid outlet", "second fluid outlet" and "third fluid outlet" of the reagent tube disclosed in the present invention actually refer to the same "fluid outlet" of the reagent tube.

[0177] The above description is merely a preferred embodiment of the present disclosure and an illustration of the technical principles employed. Those skilled in the art should understand that the scope of the invention herein is not limited to the technical solutions formed by the specific combination of the above-mentioned technical features, but also encompasses other technical solutions formed by any combination of the above-mentioned technical features or their equivalents without departing from the inventive concept. For example, a technical solution formed by replacing the above-mentioned features with (but not limited to) technical features with similar functions disclosed in this disclosure.

Claims

1. A reagent tube, characterized in that: The reagent tube comprises: a tube body having a first end and a second end, wherein the second end is configured as a closed end; a first sealing plug configured to seal the first end, wherein the first sealing plug has a first channel; The tube body, the first sealing plug and the closed second end together form an accommodating space, and the volume of the accommodating space is variable under the action of an external force; The reagent tube further includes: a second sealing plug configured to seal the second end; The second sealing plug has a liquid inlet channel and a liquid outlet channel connected to the liquid inlet channel; The reagent tube also includes a supporting conduit located in the accommodating space, one end of the supporting conduit is inserted in at least a portion of the first channel; the other end is connected to the liquid outlet channel; the other end of the supporting conduit is inserted in the liquid outlet channel, and a guide hole is opened on the tube wall of the supporting conduit, through which the supporting conduit is connected to the liquid inlet channel; the material of the tube body is PE, PVC or TPU.

2. The reagent tube according to claim 1, characterized in that The tube body is a flexible bag body.

3. The reagent tube according to claim 1, characterized in that The first sealing plug includes a first support column and a first frustum body, and the second sealing plug includes a second support column and a second frustum body, wherein the first support column and the first frustum body are formed by an integral or split molding method, and the second support column and the second frustum body are formed by an integral or split molding method.

4. The reagent tube according to claim 1, characterized in that A first fluid outlet is formed at the top of the first sealing plug, and the first fluid outlet is communicated with the first channel.

5. The reagent tube according to claim 4, characterized in that It also includes an elastic diaphragm covering the top surface of the first sealing plug, and the elastic diaphragm is configured to control the closing or opening of the first fluid outlet.

6. The reagent tube according to claim 5, characterized in that When in the closed state, the elastic diaphragm is in close contact with the first fluid outlet under the action of positive air pressure; When in the open state, a liquid flow path is formed between the elastic diaphragm and the top surface of the first sealing plug under the action of negative air pressure.

7. A multiple reagent tube, characterized in that: The multiple reagent tube comprises: Two or more reagent tubes; the reagent tubes comprising: a tube body having a first end and a second end, wherein the second end is configured as a closed end; a first sealing plug configured to seal the first end, wherein the first sealing plug has a first channel; the tube body, the first sealing plug, and the closed second end together constitute a accommodating space, and the volume of the accommodating space is variable under the action of an external force; the tube body is made of PE, PVC, or TPU; a fluid circuit valve strip configured to install and fix the reagent tubes, wherein the fluid circuit valve strip is provided with at least one transfer channel for transferring liquids between the reagent tubes; The elastic valve membrane covers at least a portion of the fluid circuit valve strip.

8. The multiple reagent tube according to claim 7, characterized in that: in: The bottom of the tube body forms the closed second end; Alternatively, the reagent tube further includes: a second sealing plug configured to seal the second end.

9. The multiple reagent tube according to claim 8, characterized in that: The reagent tube also includes a supporting conduit located in the accommodating space, one end of the supporting conduit is inserted into at least a portion of the first channel, and a first preset distance is formed between the end of the other end and the bottom of the tube body.

10. The multiple reagent tube according to claim 8, characterized in that: The second sealing plug has a liquid inlet channel and a liquid outlet channel connected to the liquid inlet channel; The reagent tube further includes a supporting conduit located in the accommodating space, one end of the supporting conduit being inserted into at least a portion of the first channel, and the other end being in communication with the liquid outlet channel.

11. The multiple reagent tube according to claim 10, characterized in that: in The other end of the supporting conduit is inserted into the liquid outlet channel, and a guide hole is provided on the tube wall of the supporting conduit, through which the supporting conduit is connected to the liquid inlet channel.

12. The multiple reagent tube according to any one of claims 7 to 11, characterized in that: The tube body is a flexible bag body.

13. The multiple reagent tube according to any one of claims 8 to 11, characterized in that: The first sealing plug includes a first support column and a first frustum body, and the second sealing plug includes a second support column and a second frustum body, wherein the first support column and the first frustum body are formed by an integral or split molding method, and the second support column and the second frustum body are formed by an integral or split molding method.

14. The multiple reagent tube according to claim 7, characterized in that: Two or more reagent tubes are fixed to the liquid valve strip at predetermined intervals, wherein: The top of the reagent tube is provided with a second fluid outlet, and the second fluid outlet is communicated with the first channel; The elastic valve membrane is configured to control the closing or opening of the second fluid outlet.

15. The multiple reagent tube according to claim 14, characterized in that: When in the open state, the elastic valve membrane forms a liquid flow path with the top of the reagent tube under the action of negative air pressure, and the liquid flow path is connected to the transfer channel, so that the liquid in at least one reagent tube is transferred to another reagent tube through at least one transfer channel.

16. A liquid transfer device, characterized in that: The device comprises: a driving unit configured to provide a power source; A cartridge body having at least one channel, wherein each channel includes at least a plurality of reagent tubes; the reagent tubes include: a tube body having a first end and a second end, wherein the second end is configured as a closed end; a first sealing plug configured to seal the first end, wherein the first sealing plug has a first channel; the tube body, the first sealing plug, and the closed second end together constitute a accommodating space, and the volume of the accommodating space is variable under the action of an external force; the tube body is made of PE, PVC, or TPU; The accommodating cavity is located below the card box body, and the accommodating cavity includes a plurality of hollow cavities corresponding to the card box body, and is used to accommodate and hold the plurality of reagent tubes.

17. The liquid transfer device according to claim 16, characterized in that in: The bottom of the tube body forms the closed second end; Alternatively, the reagent tube further includes: a second sealing plug configured to seal the second end.

18. The liquid transfer device according to claim 17, characterized in that The reagent tube also includes a supporting conduit located in the accommodating space, one end of the supporting conduit is inserted into at least a portion of the first channel, and a first preset distance is formed between the end of the other end and the bottom of the tube body.

19. The liquid transfer device according to claim 17, wherein The second sealing plug has a liquid inlet channel and a liquid outlet channel connected to the liquid inlet channel; The reagent tube further includes a supporting conduit located in the accommodating space, one end of the supporting conduit being inserted into at least a portion of the first channel; and the other end being in communication with the liquid outlet channel.

20. The liquid transfer device according to claim 19, wherein in The other end of the supporting conduit is inserted into the liquid outlet channel, and a guide hole is provided on the tube wall of the supporting conduit, through which the supporting conduit is connected to the liquid inlet channel.

21. The liquid transfer device according to any one of claims 16 to 20, characterized in that: The tube body is a flexible bag body.

22. The liquid transfer device according to any one of claims 17 to 20, characterized in that: The first sealing plug includes a first support column and a first frustum body, and the second sealing plug includes a second support column and a second frustum body, wherein the first support column and the first frustum body are formed by an integral or split molding method, and the second support column and the second frustum body are formed by an integral or split molding method.

23. The liquid transfer device according to claim 16, wherein The driving unit includes a control unit, a pneumatic valve plate and a plurality of pneumatic valves. The pneumatic valves are located at the bottom of the pneumatic valve plate, and the opening and closing of the pneumatic valves are controlled by the control unit.

24. The liquid transfer device according to claim 16 or 23, characterized in that The cartridge body includes a fluid path valve plate, wherein A plurality of the reagent tubes are fixed on the liquid circuit valve plate, and a third fluid outlet is formed at the top of the reagent tube; The liquid path valve plate is provided with a first flow path main channel, and a first sub-channel that can be selectively connected is formed between the first flow path main channel and the third fluid outlet.

25. The liquid transfer device according to claim 24, characterized in that A second flow path main channel is also provided in the liquid path valve plate, and a second sub-channel that can be selectively communicated is formed between the second flow path main channel and the third fluid outlet of at least part of the reagent tube.

26. The liquid transfer device according to claim 25, characterized in that The top surface of the reagent tube forms at least a portion of the first sub-channel and / or the second sub-channel.

27. The liquid transfer device according to claim 25 or 26, characterized in that The cartridge body further comprises a first valve membrane, The first valve membrane is laid on the top of the liquid circuit valve plate, wherein the first valve membrane at least covers the first flow path main channel, the second flow path main channel, the first sub-channel, the second sub-channel and the third fluid outlet of the reagent tube.

28. The liquid transfer device according to claim 27, characterized in that The first valve membrane is laid in an integral and / or split form.

29. The liquid transfer device according to claim 28, characterized in that The pneumatic valve comprises at least a first pneumatic valve, which together with at least a portion of the first valve membrane constitutes a first pneumatic valve membrane switch, and is configured to selectively open the first sub-channel and / or the second sub-channel.

30. The liquid transfer device according to any one of claims 16, 23, 25-26 or 28-29, characterized in that in, The multiple reagent tubes in each channel include at least a mixing pool tube, and the liquid path valve plate is provided with a pipetting channel that selectively communicates with the fluid outlet of the mixing pool tube.

31. The liquid transfer device according to claim 30, characterized in that The liquid circuit valve plate also includes a quantitative tank.

32. The liquid transfer device according to claim 31, characterized in that The liquid circuit valve plate is also provided with a switch component, which is configured to selectively connect the quantitative reservoir with the pipetting channel or the second flow path main channel.

33. The liquid transfer device according to claim 32, characterized in that The pneumatic valve further includes a second pneumatic valve configured to selectively control the opening or closing of the switch assembly.

34. The liquid transfer device according to claim 31, wherein The cartridge body further comprises at least one PCR reagent tube fixed to one side of the bottom of the liquid circuit valve plate, and the PCR reagent tube is connected to the quantitative pool.

35. The liquid transfer device according to any one of claims 31 to 34, characterized in that: The multiple reagent tubes in each channel include at least one or more of a sample tube to be tested, a lysis liquid tube, a cleaning liquid tube, a waste liquid tube, a mineral oil tube, an eluent tube, and a spare reagent tube.

36. The liquid transfer device according to any one of claims 16, 23, 25-26, 28-29 or 31-34, characterized in that The plurality of hollow cavities are respectively arranged at predetermined intervals along the first direction and the second direction of the accommodating cavity; The plurality of hollow cavities arranged along the first direction are connected to each other through a plurality of first through holes; In addition, a plurality of connection holes are opened on one side wall of the accommodating cavity, which are configured to be connected to the first power source, and the connection holes are communicated with the first through hole.

37. The liquid transfer device according to claim 36, characterized in that Part of the hollow cavity is connected through the second through hole, and the accommodating cavity further includes a mixing cavity hole configured to be connected to a second power source, and the mixing cavity hole is connected to the second through hole.

38. The liquid transfer device according to claim 37, characterized in that The first power source and the second power source are air sources providing positive and negative air pressures.

39. The liquid transfer device according to any one of claims 23, 25-26, 28-29 or 31-34, characterized in that The control unit includes a solenoid valve.

40. A method for using the liquid transfer device according to any one of claims 16 to 39, characterized in that: The method for using the liquid transfer device comprises the following steps: Pre-packaging the liquid reagents to be transferred in the reagent tubes of the cartridge body respectively; The reagent tube is placed in the accommodating cavity, and the driving unit moves downward to press the cartridge body and the accommodating cavity tightly; Under the action of the power source, the reagent tube is deformed, driving the liquid reagent in the reagent tube to transfer to other reagent tubes of the cartridge body through the first flow path main channel, thereby mixing with other reagents to form a reagent mixture.

41. The method of use according to claim 40, further comprising: The power source causes the reagent tube encapsulating the reagent mixture to be deformed, driving the reagent mixture to be transferred into the PCR reagent tube.

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