Method for detecting small rnas and applications thereof

By linking the labeled adapter sequence and performing dephosphorylation, oxidation, and phosphorylation, the problem of inaccurate detection of small RNA 3' end modifications in existing technologies has been solved. This enables accurate detection and high-throughput sequencing of sRNA 3'-OH and 3'-cP modifications, supporting the analysis of physiological and pathological changes.

CN116287159BActive Publication Date: 2026-07-07SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
Filing Date
2021-12-21
Publication Date
2026-07-07

AI Technical Summary

Technical Problem

Existing methods for detecting small RNA 3' end modifications cannot fully reflect the complete picture of intracellular sRNA, resulting in inaccurate detection.

Method used

A method for detecting sRNA terminal modifications is provided, comprising linking adapter sequences labeled 3'-OH and 3'-cP, and separating and identifying sRNAs modified with 3'-OH and 3'-cP through dephosphorylation, oxidation, and phosphorylation.

Benefits of technology

It enables accurate detection of sRNA 3'-OH and 3'-cP modifications, allows for the construction of sRNA sub-libraries, meets the needs of high-throughput sequencing, and supports the determination of physiological and pathological changes.

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Abstract

The application provides a small RNA detection method and application thereof. The application discloses a method for detecting 3' end modification of sRNA, including a TANT-seq method and a TE-qPCR method. The detection method disclosed by the application provides a new technical means for sRNA-cP detection and identification, and also provides a new idea for one-time qualitative / semi-quantitative / quantitative identification of various terminal modified sRNAs.
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