Electrospray ion source device and mass spectrometer

Abstract

The application relates to an electrospray ion source device and a mass spectrometer, the electrospray ion source device comprising: a hollow spray needle assembly, an air amplification mechanism, a headspace mechanism, the headspace mechanism being in communication with the amplification cavity, and the headspace mechanism being used for introducing compressed gas into the amplification cavity to increase the gas flow rate in the amplification cavity. In the working process of the above-mentioned electrospray ion source device, the sample forms charged droplets under the action of the hollow spray needle assembly and is sprayed into the amplification cavity through the inlet; at the same time, the headspace mechanism introduces compressed gas into the amplification cavity to increase the gas flow rate in the amplification cavity, the fast-flowing gas contacts the charged droplets, the volatilization of the solvent in the charged droplets is accelerated, the reaction molecules contact and react with the charged droplets or the gaseous sample to generate ions, and the ions or the charged droplets are driven to move towards the mass spectrometer sampling port structure, thereby improving the droplet desolvation effect and the ionization efficiency of the ion source, so as to be beneficial to improving the analysis sensitivity of the mass spectrometer.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of mass spectrometers, in particular to an electrospray ion source device and a mass spectrometer. BACKGROUND

[0002] With the development of mass spectrometry technology, electrospray ion source technology appears, and its working principle is that a sample gradually forms a Taylor cone at the outlet of an electrospray needle, and finally forms a small charged droplet away from the needle part. With the evaporation of the solvent of the droplet, the space charge effect on the surface of the droplet increases, and finally Coulomb explosion occurs, forming a smaller charged droplet. With the evaporation of the solvent, the force of the electric charge further increases, and further Coulomb explosion occurs, and the above process is repeated to finally form a charged ion.

[0003] In the conventional technology, several main factors affecting the ionization effect of the electrospray ion source are the desolvation effect and the type of reaction ions. The desolvation effect is usually achieved by heating, increasing auxiliary gas or back-blowing gas; and different reaction ions have different effects on the ionization efficiency of different substances for different application fields. Generally, the electrospray ion source is divided into a conventional ESI ion source and a nanoliter electrospray ion source, the difference between which is that the inner diameters of the spray needles of the two are different, and the nanoliter electrospray ion source relies on heating or solvent evaporation to finally form a charged ion, and the ionization efficiency of the ion source is low. SUMMARY

[0004] Therefore, it is necessary to overcome the defects of the prior art and provide an electrospray ion source device and a mass spectrometer, which can effectively improve the ionization efficiency of the ion source.

[0005] The technical scheme is as follows: an electrospray ion source device, comprising: a hollow spray needle assembly for spraying a sample to be measured; an air amplification mechanism provided with an inlet, an outlet, an air inlet and an amplification cavity, the inlet and the outlet are in communication with the amplification cavity, the hollow spray needle assembly is in communication with the inlet, and the outlet is also used for communication with a mass spectrometry sampling port structure; a headspace mechanism in communication with the amplification cavity through the air inlet, the headspace mechanism is used for introducing compressed gas into the amplification cavity to increase the gas flow rate in the amplification cavity.

[0006] In the working process of the above-mentioned electrospray ion source device, firstly, the sample forms charged droplets under the action of the hollow spray needle assembly, and is sprayed into the amplification cavity through the inlet; at the same time, the headspace mechanism introduces compressed gas into the amplification cavity, relying on the convection effect and the Venturi effect to realize the multiplication effect of the gas, form a negative pressure in the amplification cavity, and improve the gas flow rate in the amplification cavity, drive the gas in the amplification cavity to accelerate from the inlet to the gas outlet, at the same time, the rapidly flowing gas contacts the charged droplets, accelerates the volatilization of the solvent in the charged droplets, the reaction molecules will contact and react with the charged droplets or the gas sample to generate ions, and drive the ions or the charged droplets to move towards the mass spectrometer port, thereby improving the droplet desolvation effect and the ionization efficiency of the ion source, so as to improve the analysis sensitivity of the mass spectrometer.

[0007] In one of the embodiments, the headspace mechanism comprises a headspace body, an air outlet pipe and an air inlet pipe, the headspace body is provided with a headspace cavity, the headspace cavity is filled with a solution, one end of the air inlet pipe is in communication with the headspace cavity, and the other end of the air inlet pipe is used to communicate with compressed gas, and the remaining part of the headspace cavity is in communication with the amplification cavity through the air outlet pipe.

[0008] In one of the embodiments, the headspace body and the air inlet pipe are provided in two or more and one-to-one correspondence, the air outlet pipe is provided with two or more air inlet sections, the two or more air inlet sections are in communication with the two or more headspace bodies, the two or more headspace bodies are respectively filled with different solutions, and the two or more air inlet pipes are used to communicate with compressed gas.

[0009] In one of the embodiments, the solvent of the solution is one or several of acetonitrile, methanol, benzene, water, isotopic additive solution or mixed reagent.

[0010] In one of the embodiments, the hollow spray needle assembly comprises a hollow spray needle and a first power supply, the first power supply is electrically connected with the hollow spray needle, one end of the hollow spray needle extends into the inlet so that the hollow spray needle is in communication with the amplification cavity, and the hollow spray needle is used to spray the sample into the amplification cavity.

[0011] In one of the embodiments, the material of the hollow spray needle is one of quartz, glass and metal material.

[0012] In one of the embodiments, the hollow spray needle is provided with a through hole, and the diameter of the through hole is 10-300 μm.

[0013] In one of the embodiments, the air amplification mechanism comprises an air amplifier and a second power supply, the second power supply is electrically connected with the air amplifier, and the air amplifier is provided with the inlet, the outlet, the air inlet and the amplification cavity.

[0014] In one of the embodiments, the inlet and the outlet are arranged on opposite sides of the air amplification mechanism along the length direction of the air amplification mechanism, and the headspace mechanism is in communication with the circumferential side wall of the amplification cavity.

[0015] A mass spectrometer comprising the electrospray ion source device, and the outlet is in communication with the mass spectrometer inlet structure.

[0016] In the working process of the mass spectrometer, first, the sample forms charged droplets under the action of the hollow needle assembly, and is sprayed into the amplification cavity through the inlet; at the same time, the headspace mechanism introduces compressed gas into the amplification cavity, and the gas multiplication effect can be realized by relying on the Coanda effect and the Venturi effect, a negative pressure is formed in the amplification cavity, the gas flow rate in the amplification cavity is improved, the gas in the amplification cavity is accelerated from the inlet to the gas outlet, at the same time, the fast flowing gas contacts the charged droplets, accelerates the evaporation of the solvent in the charged droplets, the reaction molecules contact the charged droplets or the gas sample to produce ions, and drive the ions or the charged droplets to move to the mass spectrometer port, thereby improving the droplet evaporation effect and the ionization efficiency of the ion source, so as to improve the analysis sensitivity of the mass spectrometer. BRIEF DESCRIPTION OF DRAWINGS

[0017] The accompanying drawings, which form a part of this application, are included to provide a further understanding of the application, and are incorporated in and constitute a part of this application. The embodiments of the application illustrated in the drawings, and their description, are presented to explain the present application and are not intended to limit the present application unduly.

[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the accompanying drawings needed to be used in the embodiment description will be briefly introduced. Obviously, the accompanying drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings can also be obtained without creative labor.

[0019] Figure 1 A structural schematic diagram of the electrospray ion source device in one embodiment;

[0020] Figure 2 A structural schematic diagram of the electrospray ion source device in another embodiment;

[0021] Figure 3 A structural schematic diagram of the electrospray ion source device in another embodiment.

[0022] Explanation of the reference signs:

[0023] 100, an electrospray ion source device; 110, a hollow needle assembly; 111, a hollow needle; 112, a first power supply; 120, an air amplification mechanism; 121, an inlet; 122, an outlet; 123, an amplification cavity; 124, an air amplifier; 125, a second power supply; 126, an air inlet; 130, a headspace mechanism; 131, a headspace body; 132, an outlet tube; 1321, an inlet section; 133, an inlet tube; 134, a headspace cavity; 200, a mass spectrometry sampling port structure. DETAILED DESCRIPTION

[0024] In order to make the above objectives, features and advantages of the present application more clear and easy to understand, the specific embodiments of the present application will be described in detail below with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application. However, the present application can be practiced in a variety of ways beyond the specific embodiments described herein, and one of ordinary skill in the art can make similar improvements without departing from the scope of the present application, and therefore the present application is not limited to the specific embodiments disclosed below.

[0025] In the description of the present application, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the purpose of facilitating the description of the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.

[0026] In addition, the terms "first", "second", "third" and the like are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined with "first", "second" can explicitly or implicitly include at least one of the features. In the description of the present application, the meaning of "a plurality of" is at least two, such as two, three, etc., unless otherwise explicitly specified and limited.

[0027] In the present application, unless otherwise explicitly specified and limited, the terms "mounting", "connection", "connection", "fixing" and the like should be understood in a broad sense, for example, it can be fixed connection, or detachable connection, or integral; it can be mechanical connection, or electrical connection; it can be directly connected, or indirectly connected through an intermediate medium; it can be the internal communication of two elements or the interaction relationship between two elements, unless otherwise explicitly limited. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0028] In the present application, unless otherwise explicitly specified and limited, a first feature is "on" or "under" a second feature can be that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature is "over", "above" and "on top of" the second feature can be that the first feature is directly above or obliquely above the second feature, or only indicates that the first feature is higher in horizontal height than the second feature. The first feature is "under", "below" and "underneath" the second feature can be that the first feature is directly below or obliquely below the second feature, or only indicates that the first feature is lower in horizontal height than the second feature.

[0029] It should be noted that when an element is referred to as being "fixed to" or "set to" another element, it can be directly on the other element or there can be an intermediate element. When an element is considered to be "connected" to another element, it can be directly connected to the other element or there can be an intermediate element. The terms "vertical", "horizontal", "up", "down", "left", "right" and similar expressions used herein are only for illustrative purposes and do not represent the only implementation.

[0030] The mechanism of the electrospray ion source is not yet fully understood, and the "ion evaporation model" proposed by Iribarne and Thomson and the "charged residue model" proposed by Dole are widely supported. The sample gradually forms a Taylor cone at the outlet of the electrospray needle, and finally breaks away from the needle to form a small charged droplet. As the solvent of the droplet evaporates, the space charge effect on the surface of the droplet increases, and finally a Coulomb explosion occurs, forming smaller charged droplets. As the solvent evaporates, the charge force increases further, and further Coulomb explosion occurs, and the above process is repeated until charged ions are formed.

[0031] Several main factors affecting the ionization effect of the electrospray ion source: desolvation effect and reaction ion species. The desolvation effect is usually achieved by heating, increasing the auxiliary gas or back blowing gas; and the reaction ions are different for different application fields, and have different effects on the ionization efficiency of different substances.

[0032] Generally speaking, the electrospray ion source is divided into a conventional ESI ion source and a nanoliter electrospray ion source, the difference between the two is that the inner diameter of the spray needle is different, and the nanoliter electrospray ion source does not have auxiliary gas, and the charged ions are finally formed by heating or solvent evaporation. Without the effect of auxiliary gas, the flow rate is usually limited to tens of nanoliters per minute to several hundred nanoliters per minute.

[0033] Please refer to Figures 1 to 3 , Figure 1 Fig. 1 shows a structural schematic diagram of an electrospray ion source device 100 according to an embodiment of the present application, Figure 2Fig. 1 shows a structural schematic diagram of an electrospray ion source device 100 according to an embodiment of the present application; Figure 3 Fig. 1 shows a structural schematic diagram of an electrospray ion source device 100 according to an embodiment of the present application; the electrospray ion source device 100 comprises a hollow needle assembly 110, an air amplification mechanism 120 and a headspace mechanism 130. The hollow needle assembly 110 is used to spray the sample to be measured. The air amplification mechanism 120 is provided with an inlet 121, an outlet 122, an air inlet 126 and an amplification cavity 123, and the inlet 121 and the outlet 122 are both in communication with the amplification cavity 123. The hollow needle assembly 110 is in communication with the inlet 121, and the outlet 122 is also used to communicate with a mass spectrometry inlet structure 200. The headspace mechanism 130 is in communication with the amplification cavity 123 through the air inlet 126, and the headspace mechanism 130 is used to introduce compressed gas into the amplification cavity 123 to increase the gas flow rate in the amplification cavity 123.

[0034] In the working process of the above-mentioned electrospray ion source device 100, first, the sample forms charged droplets under the action of the hollow needle assembly 110 and is sprayed into the amplification cavity 123 through the inlet 121; at the same time, the headspace mechanism 130 introduces compressed gas into the amplification cavity 123, relying on the Coanda effect and the Venturi effect to achieve the multiplication effect of the gas, form a negative pressure in the amplification cavity 123, increase the gas flow rate in the amplification cavity 123, drive the gas in the amplification cavity 123 to accelerate from the inlet 121 to the gas outlet 122, and at the same time, the rapidly flowing gas contacts the charged droplets to accelerate the volatilization of the solvent in the charged droplets, the reaction molecules will contact the charged droplets or the gas sample to produce ions, and drive the ions or the charged droplets to move to the mass spectrometry inlet structure 200, thereby improving the droplet desolvation effect and the ionization efficiency of the ion source, so as to improve the analysis sensitivity of the mass spectrometer.

[0035] Optionally, the way that the hollow needle assembly 110 is in communication with the inlet 121 can be that the diameter of the inlet 121 is greater than the diameter of the hollow needle assembly 110, the hollow needle assembly 110 extends into the inlet 121 and is in gap cooperation with the wall of the inlet 121, or the hollow needle assembly 110 is located outside the inlet 121 and is in gap cooperation with the inlet 121, the hollow needle assembly 110 can spray the charged droplets into the inlet 121 to enter the amplification cavity 123, and after the amplification cavity 123 forms a negative pressure, the gas in the environment can enter the amplification cavity 123 through the inlet 121.

[0036] It should be noted that the headspace mechanism 130 used to introduce compressed gas into the amplification cavity 123 should be understood as that the headspace mechanism 130 can generate compressed gas by itself and introduce it into the amplification cavity 123, or the headspace mechanism 130 is in communication with a compressed gas device, and the compressed gas flows to the amplification cavity 123 through the headspace device.

[0037] Specifically, refer to Figure 1 , Figure 2 and Figure 3 , the headspace mechanism 130 comprises a headspace body 131, an air outlet pipe 132 and an air inlet pipe 133. The headspace body 131 is provided with a headspace cavity 134, the headspace cavity 134 is filled with a solution, one end of the air inlet pipe 133 is communicated with the headspace cavity 134, the other end of the air inlet pipe 133 is used for communicating with compressed gas, and the remaining part of the headspace cavity 134 is communicated with the amplification cavity 123 through the air outlet pipe 132. In this way, the compressed gas is introduced into the solution in the headspace body 131, so that the solution volatilizes and enters the amplification cavity 123 with the compressed gas, and the volatilized gas can contact and react with the charged droplets in the amplification cavity 123, thereby facilitating the improvement of the ionization effect.

[0038] It should be noted that the headspace cavity 134 is filled with a solution, and the remaining part of the headspace cavity 134 is communicated with the amplification cavity 123 through the air outlet pipe 132, which means that the solution is filled in the headspace cavity 134, but not full of the headspace cavity 134, and the remaining part of the headspace cavity 134 is air. The air outlet pipe 132 extends into the headspace cavity 134 and is arranged to be spaced apart from the solution surface, so that the solution does not enter the amplification cavity 123 through the air outlet pipe 132.

[0039] The headspace body 131 can be a flask, a sealed tank or other sealed device.

[0040] Specifically, refer to Figure 1 , the headspace body 131 is a sealed tank, and the top of the sealed tank is provided with two openings for communicating with the air outlet pipe 132 and the air inlet pipe 133. In this way, it is beneficial to ensure the structural stability and overall quality of the headspace body 131, thereby improving the service life of the electrospray ion source device 100. The present embodiment only provides a specific implementation of the headspace body 131, but is not limited thereto.

[0041] Optionally, the solvent of the solution can be acetonitrile, methanol, benzene, water, isotopic additive solution or other solvents. In the present embodiment, the solvent of the solution is methanol, but is not limited thereto.

[0042] Optionally, the communication mode of the air inlet pipe 133 and the headspace cavity 134 can be that the air inlet pipe is communicated with the remaining part of the headspace cavity 134, or the air inlet pipe 133 extends into the solution.

[0043] Specifically, refer to Figure 2 , the air inlet pipe 133 extends into the solution. One end of the air outlet pipe 132 is arranged to be spaced apart from the liquid surface of the solution. In this way, it is beneficial to accelerate the volatilization of ions in the solution, thereby improving the concentration of reaction ions in the compressed gas and enhancing the ionization effect of the charged droplets in the amplification cavity 123.

[0044] Further, refer toFigure 2 With Figure 3 The two or more headspace bodies 131 and the two or more gas inlet tubes 133 are one-to-one corresponding, and the gas outlet tube 132 is provided with two or more gas inlet sections 1321, the two or more gas inlet sections 1321 are communicated with the two or more headspace bodies 131, the two or more headspace bodies 131 are respectively filled with different solutions, and the two or more gas inlet tubes 133 are used to communicate the compressed gas. In this way, multiple reaction molecules can be introduced into the amplification cavity 123 to contact and react with the charged droplets or gas samples, accelerate the solvent evaporation of the charged droplets, effectively expand the ionization types and range, and improve the ionization efficiency.

[0045] In one embodiment, referring to Figure 1 The hollow needle assembly 110 includes a hollow needle 111 and a first power supply 112. The first power supply 112 is electrically connected with the hollow needle 111, one end of the hollow needle 111 extends into the inlet 121, so that the hollow needle 111 is communicated with the amplification cavity 123. The hollow needle 111 is used to spray the sample into the amplification cavity 123. In this way, the measured sample enters the tip through the hollow needle 111, and under the action of the first power supply 112, the measured sample forms charged droplets, and then is sprayed into the amplification cavity 123.

[0046] Specifically, referring to Figure 1 The hollow needle 111 extends into the inlet 121, and the hollow needle 111 is in clearance fit with the inner wall of the inlet 121. Further, the hollow needle 111, the inlet 121 and the air amplification mechanism 120 are coaxially arranged. In this way, the ionization efficiency can be further improved.

[0047] Optionally, referring to Figure 1 The material of the hollow needle 111 is one of quartz, glass or metal material.

[0048] Specifically, in the embodiment, referring to Figure 1 The material of the hollow needle 111 is metal material, but it is not limited thereto.

[0049] Further, referring to Figure 1 The hollow needle 111 is provided with a through hole, and the diameter of the through hole is 10 μm-300 μm. In this way, the sample can gradually form a Taylor cone at the outlet 122 of the hollow needle 111, and finally separate from the hollow needle 111 to form smaller charged droplets.

[0050] In one embodiment, referring to Figure 1The air amplification mechanism 120 comprises an air amplifier 124 and a second power supply 125 electrically connected to the air amplifier 124, and the air amplifier 124 is provided with an inlet 121, an outlet 122, an air inlet 126 and an amplification cavity 123. In this way, through the action of the air amplifier 124, the desolvation of the nanoliter ion source droplet is realized, the ionization efficiency is optimized, the flow rate range of the electrospray ion source is expanded from the nanoliter level to the nanoliter to microliter level, and the gas multiplication effect can be realized relying on the Coanda effect and the Venturi effect. The gas flow at the outlet 122 position of the air amplifier 124 is several times or even dozens of times of the input gas flow rate, and the high-speed gas flow effectively accelerates the desolvation of the spray and improves the ionization efficiency.

[0051] In one embodiment, referring to Figure 1 , the inlet 121 and the outlet 122 are oppositely arranged on the opposite sides of the air amplification mechanism 120 along the length direction of the air amplification mechanism 120, and the headspace mechanism 130 is in communication with the circumferential side wall of the amplification cavity 123. In this way, the reaction molecules in the headspace mechanism 130 entering the amplification cavity 123 can move along the inner wall of the amplification cavity 123 with the compressed gas, accelerate the gas flow rate in the amplification cavity 123, form a negative pressure at the inlet 121, drive the gas in the amplification cavity 123 from the inlet 121 to the outlet 122, and move the gas in contact with the charged droplets to accelerate the volatilization of the solvent in the charged droplets. The reaction molecules will react with the charged droplets or gas samples to produce ions, and drive the ions or charged droplets to move towards the mass spectrometry inlet structure 200.

[0052] For further understanding and description of the length direction of the amplification mechanism 120, please refer to Figure 1 , the length direction of the amplification mechanism 120 is Figure 1 the direction indicated by any one of the arrows on the straight line S1.

[0053] In one embodiment, a hollow needle 111 is a metal capillary, a direct current voltage with an amplitude of 4000V is applied to the metal capillary by a first power supply 112, a direct current voltage with an amplitude of 1000V is applied to the air amplifier 124 by a second power supply 125, and a voltage of 120V is applied to the mass spectrometry inlet structure 200. A potential difference is formed between the metal capillary, the air amplifier 124, and the mass spectrometry inlet structure 200, which promotes the movement of ions to the mass spectrometry inlet structure 200. The tip of the hollow needle 111 is located 6 mm axially inside the inlet 121 of the air amplifier 124, and the inlet 121 of the metal capillary is located 15 mm axially inside the outlet 122 of the air amplifier 124. The inner diameter of the metal capillary is 100 μm, the flow rate of a reserpine sample in methanol is selected to be 1 uL / min, a Taylor cone is formed at the tip of the metal capillary, and finally a charged spray is formed. Compressed air enters the headspace body 131 at a rate of 4 L / min, the headspace body 131 contains a methanol solution, and methanol molecules enter the amplification cavity 123 with the compressed air. Under the action of the wall effect, a negative pressure is formed at the gas inlet 121 of the air amplifier 124, which drives the gas in the environment into the air amplifier 124. A large amount of gas contacts the charged droplets, accelerates the solvent evaporation of the charged droplets, and further improves the ionization efficiency.

[0054] In another embodiment, a high-sensitivity electrospray ion source device 100, a hollow needle 111 is a quartz capillary, a direct current voltage with an amplitude of 3000V is applied to the quartz capillary by a first power supply 112, a direct current voltage with an amplitude of 300V is applied to the air amplifier 124 by a power supply, and a voltage of 80V is applied to the mass spectrometry inlet structure 200. A potential difference is formed between the quartz capillary, the air amplifier 124, and the mass spectrometry inlet structure 200, which promotes the movement of ions to the mass spectrometry inlet structure 200. The tip of the quartz capillary is located 6 mm axially inside the inlet 121 of the air amplifier 124, and the inlet 121 of the quartz capillary is located 15 mm axially inside the outlet 122 of the air amplifier 124. The inner diameter of the quartz capillary is 80 μm, the flow rate of a protein sample in methanol is selected to be 0.7 uL / min, a Taylor cone is formed at the tip of the metal capillary, and finally a charged spray is formed. The headspace device includes three headspace bodies 131, each containing methanol, isotopic additive solution, and acetonitrile, respectively. Compressed air enters at a rate of 2 L / min, and the volatilized molecules of the three solvents are mixed and enter the amplification cavity 123 of the air amplifier 124 with the compressed air. Under the action of the wall effect, a negative pressure is formed at the inlet 121 of the air amplifier 124, which drives the gas in the environment into the air amplifier 120. A large amount of gas contacts the charged droplets, accelerates the solvent evaporation of the charged droplets, and improves the ionization efficiency.

[0055] In one embodiment, a mass spectrometer (not shown in the figure) comprises the electrospray ion source device 100 of any of the above embodiments, and the outlet 122 is in communication with a mass spectrometer inlet structure 200.

[0056] During operation of the above mass spectrometer, first, the sample is subjected to the hollow needle assembly 110 to form charged droplets, and is sprayed into the amplification chamber 123 through the inlet 121; at the same time, the headspace mechanism 130 introduces compressed gas into the amplification chamber 123, relying on the Coanda effect and the Venturi effect to achieve the effect of multiplying the gas, forming a negative pressure in the amplification chamber 123, improving the gas flow rate in the amplification chamber 123, driving the gas in the amplification chamber 123 to accelerate from the inlet 121 to the gas outlet 122, at the same time, the rapidly flowing gas contacts the charged droplets, accelerating the evaporation of the solvent in the charged droplets, the reaction molecules will contact and react with the charged droplets or gas samples to produce ions, and drive the ions or charged droplets to move towards the mass spectrometer inlet structure 200, thereby improving the droplet desolvation effect and the ionization efficiency of the ion source, thereby improving the analysis sensitivity of the mass spectrometer.

[0057] The technical features of the above embodiments can be combined in any manner. To make the description concise, not all possible combinations of the technical features in the above embodiments are described, but as long as the combinations of the technical features do not contradict, they should be considered within the scope of the present disclosure.

[0058] The above embodiments only express several embodiments of the present application, and the description is more specific and detailed, but it should not be understood as a limitation on the scope of the patent. It should be pointed out that for ordinary skilled in the art, without departing from the concept of the present application, a number of modifications and improvements can be made, which are within the scope of the present application. Therefore, the scope of protection of the present application should be subject to the appended claims.

Claims

1. An electrospray ionization source device, characterized in that, The electrospray ion source device includes: A hollow nozzle assembly, wherein the hollow nozzle assembly is used to eject the sample to be tested; An air amplification mechanism is provided with an inlet, an outlet, an air inlet, and an amplification cavity. The inlet, the outlet, and the air inlet are all connected to the amplification cavity. The hollow nozzle assembly is connected to the inlet, and the outlet is also used to connect to the mass spectrometer injection port structure. A headspace mechanism includes a headspace body, an outlet pipe, and an inlet pipe. The headspace body has a headspace cavity containing a reaction solution. After evaporation, the reaction solution reacts with charged droplets in the amplification cavity to generate ions. One end of the inlet pipe is connected to the headspace cavity, and the other end is connected to compressed gas to increase the gas flow rate in the amplification cavity. The remaining portion of the headspace cavity is connected to the amplification cavity through the outlet pipe. The outlet pipe is used to pass the volatile reaction solution gas along with the compressed gas into the amplification cavity.

2. The electrospray ion source device according to claim 1, characterized in that, The headspace body and the air inlet pipe are both provided in two or more corresponding configurations. The air outlet pipe has two or more air inlet sections. The two or more air inlet sections are connected to two or more headspace bodies. The two or more headspace bodies are respectively filled with different reaction solutions. The two or more air inlet pipes are all used to connect compressed gas.

3. The electrospray ion source device according to claim 2, characterized in that, The solvents of the reaction solution are one or more of acetonitrile, methanol, benzene, and water.

4. The electrospray ion source device according to claim 1, characterized in that, The hollow nozzle assembly includes a hollow nozzle and a first power supply. The first power supply is electrically connected to the hollow nozzle. One end of the hollow nozzle extends into the inlet so that the hollow nozzle communicates with the amplification cavity. The hollow nozzle is used to spray the sample into the amplification cavity.

5. The electrospray ion source device according to claim 4, characterized in that, The hollow nozzle is made of one of the following materials: quartz, glass, or metal.

6. The electrospray ion source device according to claim 4, characterized in that, The hollow nozzle is provided with a through hole, the diameter of which is 10μm~300μm.

7. The electrospray ion source device according to claim 1, characterized in that, The headspace body is a flask or a sealed container.

8. The electrospray ionization source device according to any one of claims 1-7, characterized in that, The air amplification mechanism includes an air amplifier and a second power supply, the second power supply being electrically connected to the air amplifier, and the air amplifier having the inlet, the outlet, the air inlet and the amplification cavity.

9. The electrospray ionization source device according to claim 8, characterized in that, The inlet and the outlet are disposed opposite each other on opposite sides of the air amplification mechanism along the length of the air amplification mechanism, and the headspace mechanism is connected to the circumferential sidewall of the amplification cavity.

10. A mass spectrometer, characterized in that, The mass spectrometer includes the electrospray ionization source device according to any one of claims 1-9, and the outlet is connected to the mass spectrometer inlet structure.

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