A rapid dehydration and transparency method for pathological sections
By using ethanol solutions of gradually increasing concentrations and fatty alcohol polyoxyethylene ethers for dehydration and clearing of pathological sections, the problems of long dehydration time and the use of xylene are solved, enabling rapid and safe preparation of pathological sections.
CN116358950BActive Publication Date: 2026-07-17BEIJING ENRUIKANG AGRI TECH CONSULTING CO LTD
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- BEIJING ENRUIKANG AGRI TECH CONSULTING CO LTD
- Filing Date
- 2021-12-28
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
The current process for preparing pathological slides involves a long dehydration time and the use of harmful xylene as a clearing agent, posing health risks and affecting work efficiency.
Method used
Dehydration is achieved using an ethanol solution with gradually increasing volume concentration, followed by a clearing process using fatty alcohol polyoxyethylene ether at 55–65°C. Microwave heating is then used to improve solvent penetration, thus replacing traditional xylene.
Benefits of technology
It significantly shortens the dehydration and clearing time, avoids the use of xylene, ensures the quality of pathological sections, and improves processing efficiency.
✦ Generated by Eureka AI based on patent content.
Smart Images
Figure CN116358950B_ABST
Abstract
The application provides a rapid dehydration and transparency method of pathological sections, and belongs to the technical field of tissue sample preparation. The rapid dehydration and transparency method comprises the following steps: dehydrating an in-vitro tissue by using ethanol solutions with volume concentrations from low to high, and then transparently treating the dehydrated in-vitro tissue in a fatty alcohol polyoxyethylene ether, wherein the temperature of the dehydration treatment and the transparency treatment is 55-65 DEG C. The application sets the temperature of the dehydration treatment and the transparency treatment as 55-65 DEG C, which significantly shortens the time of the dehydration treatment and the transparency treatment. The application replaces xylene, which is used in the traditional preparation of pathological sections, with a fatty alcohol polyoxyethylene ether, which not only avoids the risk of cancer caused by long-term contact with xylene, but also achieves the same transparency effect as xylene. Experimental results show that, compared with the traditional method, the pathological sections prepared by the application not only significantly shorten the time of dehydration and transparency treatment, but also do not affect the section quality due to the shortened time.
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