Mixed reagent preparation method and system

Abstract

This application discloses a method and system for preparing a mixed reagent, relating to the field of medical temperature control technology, and particularly to a method and system for preparing a mixed reagent. The method includes: providing at least two reagents to be mixed, including a temperature control reagent, according to a target mixing ratio; mixing a first dose of the temperature control reagent with a specified dose of other component reagents to obtain a first mixed reagent; wherein the first dose is the sum of a first specified dose and a temperature control dose, and the first specified dose and the specified dose satisfy the specified ratio; adding the remaining doses of the temperature control reagent and the remaining doses of other component reagents to the first mixed reagent in batches to obtain the target mixed reagent. This application improves the quality of the prepared mixture.

Description

Technical Field

[0001] This application relates to the field of medical temperature control technology, and in particular to a method and system for preparing mixed reagents. Background Technology

[0002] In related technologies, a "hot-to-cold" method can be used to mix heated temperature-controlled reagents with other unheated reagents to maintain the temperature during the reaction process. In low-temperature environments, the reaction tank and the reagents inside dissipate heat quickly. To ensure the temperature of the mixture, the heated temperature-controlled reagent usually needs to be preheated to a relatively higher temperature in the liquid path to ensure that the temperature of the temperature-controlled reagent remains within the required temperature range throughout its time in the reaction tank.

[0003] However, if the temperature of the temperature-controlled reagent is too high, it will increase the number of bubbles in the reagent, leading to inaccurate quantification. At the same time, excessively high preheating temperature may also cause the reagent to denature, resulting in poor quality of the prepared mixture.

[0004] Application content

[0005] The main objective of this application is to provide a method, apparatus, equipment, and storage medium for preparing mixed reagents, aiming to solve the technical problem of poor quality in the preparation of mixed solutions.

[0006] Firstly, to achieve the above objectives, this application provides a method for preparing a mixed reagent, the method comprising:

[0007] Based on the mixing ratio of the target mixed reagents, at least two reagents to be mixed are provided, including a temperature-controlled reagent;

[0008] A first mixed reagent is obtained by mixing a first dose of temperature-controlled reagent with a specified dose of other component reagents; wherein, the first dose is the sum of the first specified dose and the temperature-controlled dose, and the first specified dose and the specified dose satisfy the specified ratio.

[0009] The remaining dose of temperature-controlled reagent and the remaining dose of each of the other component reagents are added to the first mixed reagent in batches to obtain the target mixed reagent.

[0010] Optionally, the first mixed reagent, obtained by mixing the first dose of the temperature-controlled reagent with the other component reagents in the specified dosage, includes:

[0011] A first dose of the first reagent and a second dose of the second reagent are mixed to obtain the first mixed reagent; wherein, the first dose is the sum of the first ratio dose and the temperature-controlled dose, and the first ratio dose and the second dose satisfy the ratio described above;

[0012] The step of adding the remaining dose of temperature-controlled reagent and the remaining dose of each of the other component reagents to the first mixed reagent in batches to obtain the target mixed reagent includes:

[0013] The remaining doses of the first reagent and the remaining doses of the second reagent are added to the first mixed reagent in batches to obtain the target mixed reagent.

[0014] Optionally, the step of adding the remaining doses of the first reagent and the remaining doses of the second reagent to the first mixed reagent in batches to obtain the target mixed reagent includes:

[0015] According to the first ratio dosage, the remaining dose of the first reagent is added to the first mixed reagent in N portions to obtain the target mixed reagent; wherein the dose added in one portion is the difference between the first ratio dosage and the temperature-controlled dosage;

[0016] According to the second dose, the remaining dose of the second reagent is divided into N portions and added to the first mixed reagent on an average basis to obtain the target mixed reagent; where N is a positive integer greater than or equal to 1.

[0017] Optionally, the total number of times the reagent to be mixed is added is T, where T satisfies: 2≤T≤5.

[0018] Optionally, the temperature-controlled dose satisfies a preset ratio relative to the first ratio dose, the preset ratio being P, wherein P satisfies: 0 < P ≤ 1 / 2.

[0019] Optionally, P specifically satisfies: 0 < P ≤ 1 / 3.

[0020] Optionally, P is 1 / 5.

[0021] Secondly, this application also provides a mixed reagent preparation system, the system comprising:

[0022] Reaction tank;

[0023] At least two reagent addition branches are connected to the reaction vessel. The reagent addition branches correspond one-to-one with the quantity of the reagents to be mixed in the reaction vessel. One of the reagent addition branches is a temperature-controlled reagent addition branch.

[0024] A controller is configured to, upon the first addition, control the temperature-controlled reagent addition branch to add a first dose of temperature-controlled reagent to the reaction tank, and control the other reagent addition branches to add the other component reagents in the specified dosage to the reaction tank, thereby obtaining a first mixed reagent; wherein, the first dose is the sum of the first specified dosage and the temperature-controlled dosage, and the first specified dosage and the specified dosage satisfy the specified ratio;

[0025] The controller is also used to control the temperature-controlled reagent addition branch to add the remaining dose of the first reagent to the reaction tank in batches, and to simultaneously control the other reagent addition branches to add the remaining doses of other component reagents to the reaction tank to obtain the target mixed reagent.

[0026] Optionally, the at least two reagent addition branches include:

[0027] A first reagent addition branch is connected to the reaction cell and is used to add a first reagent to the reaction cell;

[0028] The second reagent addition branch is connected to the reaction cell and is used to add a second reagent to the reaction cell.

[0029] The controller is specifically used to control the first reagent addition branch to add a first dose of the first reagent to the reaction cell, and to control the second reagent addition branch to add a second dose of the second reagent to the reaction cell, so that the first dose of the first reagent and the second dose of the second reagent are mixed to obtain a first mixed reagent;

[0030] The controller is specifically used to control the first reagent addition branch and the second reagent addition branch to add the remaining doses of the first reagent and the remaining doses of the second reagent to the reaction tank in batches to obtain the target mixed reagent, wherein the first dose is the sum of the first ratio dose and the temperature control dose, and the first ratio dose and the second dose satisfy the ratio.

[0031] Optionally, the first reagent addition branch includes a first negative pressure extractor;

[0032] The second reagent addition branch includes a second negative pressure extractor.

[0033] This application provides a method for preparing a mixed reagent. Based on a target mixed reagent ratio, at least two reagents to be mixed are provided, including a temperature-controlled reagent. A first dose of the temperature-controlled reagent and a specified dose of each of the other component reagents are mixed to obtain a first mixed reagent. The first dose is the sum of a first specified dose and a temperature-controlled dose, and the first specified dose and the specified dose satisfy the specified ratio. The remaining doses of the temperature-controlled reagent and the remaining doses of each of the other component reagents are added to the first mixed reagent in batches to obtain the target mixed reagent.

[0034] Therefore, this application mixes the temperature-controlled reagent and other component reagents in batches, and appropriately increases the dosage of the temperature-controlled reagent during the first addition so that the excess heat is absorbed by the mixing environment, i.e., the reaction tank, which heats the reaction tank and ensures the temperature of the mixture. This eliminates the need to heat the temperature-controlled reagent separately to the highest heating temperature, avoids the problem of inaccurate quantification caused by increased bubbles in the temperature-controlled reagent, and avoids the problem of denaturation of the temperature-controlled reagent, thereby improving the preparation quality of the mixture. Attached Figure Description

[0035] Figure 1 This is a schematic diagram of the architecture of the mixed reagent preparation system of the mixed reagent preparation method of this application;

[0036] Figure 2 This is a schematic flowchart of the first embodiment of the mixed reagent preparation method of this application.

[0037] The realization of the purpose, functional features and advantages of this application will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0038] It should be understood that the specific embodiments described herein are merely illustrative of this application and are not intended to limit this application.

[0039] Due to current technology, medical devices commonly use multiple liquid reagents, and the reaction between samples and reagents needs to occur within a specific temperature range. Therefore, temperature control of the reagents is necessary to maintain the temperature during the reaction process. For temperature control systems with multiple reagents (at least two) entering the reaction cell, each reagent is typically heated separately, or the reaction cell is also insulated to maintain a stable temperature of the mixture during the reaction. This involves heating the multiple reagents entering the reaction cell and heating and insulating the reaction cell to maintain a stable temperature during the reaction and detection process, ensuring the accuracy of the detection results. However, based on previous operations, heating each reagent separately, heating the reaction cell, and then sending all the heated reagents into the reaction cell to maintain a stable temperature requires heating each reagent individually. This heating structure is complex and costly. Because multiple liquid paths need to be heated simultaneously, the heating components are large, have many branches, and occupy more space, which is not conducive to the modular design of the heating components and the miniaturization of the instrument.

[0040] When using DIFF reagents, they are typically heated to a high temperature, then dispensed all at once and directly mixed with cold diluent to achieve the desired mixture temperature. This involves heating a single reagent stream to a high temperature and mixing it with other unheated reagents, using a "hot-to-cold" method to achieve temperature control. In some technical solutions, the DIFF reagent heated to a very high temperature is directly mixed with room-temperature diluent to obtain the desired mixture temperature. This approach is relatively simple, but it only heats the reagent in a single stream, and the reagent must be added in a single batch directly into the reaction tank. In practical applications, considering the limited amount of reagent in the reaction tank, adding all reagent at once results in a relatively long reaction time. In low-temperature environments, the reaction tank and its internal reagents dissipate heat quickly. To maintain the mixture temperature, the DIFF reagent usually needs to be preheated to a relatively high temperature in the stream to ensure that the reagent temperature remains within the required range throughout its time in the reaction tank. However, excessively high reagent temperatures can increase air bubbles, leading to inaccurate quantification. At the same time, excessively high preheating temperatures may also lead to problems such as reagent denaturation.

[0041] Therefore, this application provides a solution to mix the temperature-controlled reagent and other component reagents in batches, and to appropriately increase the dosage of the temperature-controlled reagent when adding it for the first time, so that the excess heat is absorbed by the mixing environment, i.e., the reaction tank, which heats the reaction tank and ensures the temperature of the mixture. This eliminates the need to heat the temperature-controlled reagent separately to the highest heating temperature, avoids the problem of inaccurate quantification caused by increased bubbles in the temperature-controlled reagent, and avoids the problem of denaturation of the temperature-controlled reagent, thereby improving the preparation quality of the mixture.

[0042] The following embodiments of this application will describe the mixed reagent preparation system used in the technical implementation of this application:

[0043] Reference Figure 1 , Figure 1 This is a schematic diagram of the architecture of a mixed reagent preparation system provided in an exemplary embodiment. Figure 1 As shown, the mixed reagent preparation system may include a reaction tank 11, at least two reagent addition branches 12, and a controller 13.

[0044] The reaction chamber 11 can be a container for carrying reagents and reacting with samples.

[0045] At least two reagent adding branches 12 are connected to the reaction cell. The number of reagents added to each branch is consistent with the number of reagents to be mixed and they correspond one-to-one. The branches are used to add the corresponding reagents to be mixed to the reaction cell. One of the reagent adding branches is a temperature-controlled reagent adding branch.

[0046] The temperature-controlled reagent adding branch is equipped with a heating module to heat the temperature-controlled reagent.

[0047] The controller 13 is used to control the temperature-controlled reagent addition branch to add a first dose of temperature-controlled reagent to the reaction tank during the first addition, and to control the other reagent addition branches to add the other component reagents of the specified dosage to the reaction tank to obtain a first mixed reagent; wherein, the first dosage is the sum of the first specified dosage and the temperature-controlled dosage, and the first specified dosage and the specified dosage satisfy the specified ratio.

[0048] The controller 13 is also used to control the temperature-controlled reagent addition branch to add the remaining dose of the first reagent to the reaction tank in batches, and to simultaneously control the other reagent addition branches to add the remaining dose of each of the other component reagents to the reaction tank to obtain the target mixed reagent.

[0049] Therefore, in this embodiment, the temperature control reagent and other component reagents are mixed in batches, and the dosage of the temperature control reagent is appropriately increased when adding the temperature control reagent for the first time, so that the excess heat is absorbed by the environment in which the mixing takes place, i.e., the reaction tank, which heats the reaction tank and ensures the temperature of the mixture. This eliminates the need to heat the temperature control reagent separately to the highest heating temperature, avoids the problem of inaccurate quantification caused by the increase of bubbles in the temperature control reagent, and avoids the problem of denaturation of the temperature control reagent, thereby improving the preparation quality of the mixture.

[0050] Furthermore, compared to the existing technology that heats multiple reagents separately, this embodiment only heats the temperature-controlled reagent. In the case of multiple reagents participating in the reaction, the structure of the heating module is simplified, the volume of the heating module is reduced, and the cost of the components is lowered.

[0051] Furthermore, since the temperature of the first mixed reagent obtained after the first mixing is slightly higher, it can promote the reaction. Although the second hot temperature control reagent is less, the reaction tank has already been heated, so the impact is not significant. Therefore, the temperature of the mixed solution is more stable, which is more conducive to the reaction and ensures accurate measurement results.

[0052] Furthermore, compared to the existing technology of heating a single reagent to a high temperature and adding it to the reaction tank all at once, this embodiment uses a batch mixing method when mixing the temperature-controlled reagent with other component reagents. The dosage of the temperature-controlled reagent is appropriately increased during the first addition so that the excess heat is absorbed by the mixing environment, i.e., the reaction tank, which heats the reaction tank and ensures the temperature of the mixture. This effectively reduces the maximum heating temperature of the temperature-controlled reagent and significantly improves the quantitative problems caused by the precipitation of bubbles due to high temperature and the denaturation problems caused by excessively high temperature of the temperature-controlled reagent.

[0053] Furthermore, in one embodiment, the at least two reagent addition branches 12 include:

[0054] First reagent addition branch 121, the first reagent addition branch is connected to the reaction cell, and is used to add a first reagent to the reaction cell;

[0055] Second reagent addition branch 122; the second reagent addition branch is connected to the reaction cell and is used to add a second reagent to the reaction cell;

[0056] Controller 13 is specifically used to control the first reagent addition branch to add a first dose of the first reagent to the reaction cell, and to control the second reagent addition branch to add a second dose of the second reagent to the reaction cell, so that the first dose of the first reagent and the second dose of the second reagent are mixed to obtain a first mixed reagent;

[0057] The controller 13 is specifically used to control the first reagent addition branch and the second reagent addition branch to add the remaining doses of the first reagent and the remaining doses of the second reagent to the reaction tank in batches to obtain the target mixed reagent, wherein the first dose is the sum of the first ratio dose and the temperature control dose, and the first ratio dose and the second dose satisfy the ratio.

[0058] Furthermore, in one embodiment, the first reagent addition branch includes a first negative pressure extractor; the second reagent addition branch includes a second negative pressure extractor.

[0059] Based on, but not limited to, the above-described mixed reagent preparation system, this application provides a first embodiment of a mixed reagent preparation method. (Refer to...) Figure 2 , Figure 2 A schematic flowchart of the first embodiment of the mixed reagent preparation method of this application is shown.

[0060] It should be noted that although the logical order is shown in the flowchart, in some cases, the steps shown or described may be performed in a different order than that shown here.

[0061] In this embodiment, the method includes:

[0062] S10. Based on the mixing ratio of the target mixed reagents, provide at least two reagents to be mixed, including a temperature-controlled reagent.

[0063] It is important to understand that the target mixture is specifically a mixture formed by adding the sample and at least two reagents to be mixed together into the reaction vessel. The ratio of the target mixture is the ratio between multiple reagents to be mixed, which can be the weight ratio, mass ratio, or mass percentage of the multiple reagents to be mixed.

[0064] The temperature-controlled reagent is specifically a reagent that needs to be preheated to adjust the temperature of the mixture to a preset target temperature. The preset target temperature can be set within the range of 35°C to 39°C; in this embodiment, the target mixture temperature is within the range of 36°C to 38°C.

[0065] S20. Mix the first dose of temperature-controlled reagent with the other component reagents of the specified dosage to obtain a first mixed reagent; wherein, the first dose is the sum of the first specified dosage and the temperature-controlled dose, and the first specified dosage and the specified dosage satisfy the specified ratio.

[0066] It is important to understand that this step involves adding the reagents to be mixed into the reaction vessel for the first time. The first dose is the initial dose of temperature-controlled reagent added. The first dose is the sum of the first ratio reagent and the temperature-controlled reagent dose. Specifically, the first ratio dose is the initial dose of temperature-controlled reagent allocated by the user based on the total number of times the reagents to be mixed will be added.

[0067] The dosage ratio refers to the initial dosage of each of the other reagent components. It is understood that each of the other reagent components has its own dosage ratio. The initial dosage ratio must satisfy the proportions of all other reagent components to ensure that the reaction system in the reaction vessel is not altered.

[0068] In related technologies, the reaction tank is not heated before the hot reagent, i.e. the temperature-controlled reagent in this embodiment, enters the reaction tank. When the hot reagent enters the reaction tank, some of its heat will be carried away by the reaction tank. Therefore, in this embodiment, in order to ensure that the target mixed reagent reaches the preset target temperature, it is necessary to appropriately increase the dosage of the temperature-controlled reagent while ensuring the mixing ratio, so as to reserve some heat for the reaction tank to absorb heat.

[0069] S30. The remaining dose of the first reagent and the remaining dose of each of the other component reagents are added to the first mixed reagent in batches to obtain the target mixed reagent.

[0070] It is important to understand that after mixing the first dose of temperature-controlled reagent with the other components of the reagent in the specified dosage to obtain the first mixed reagent, the remaining dose of the first kit and other reagents to be mixed are added to the first mixed reagent in batches according to the preset addition number, until the mixed solution is completely added, and the target mixed reagent is obtained.

[0071] In this embodiment, the temperature control reagent and other component reagents are mixed in batches. The dosage of the temperature control reagent is appropriately increased when adding it for the first time, so that the excess heat is absorbed by the mixing environment, i.e., the reaction tank. This heats the reaction tank and ensures the temperature of the mixture. Therefore, it is not necessary to heat the temperature control reagent separately to the highest heating temperature. This avoids the problem of inaccurate quantification caused by the increase of bubbles in the temperature control reagent and the problem of denaturation of the temperature control reagent, thereby improving the preparation quality of the mixture.

[0072] The following description uses an example where two reagents are to be mixed. As an example, step S20 specifically includes:

[0073] S201. Mix the first dose of the first reagent and the second dose of the second reagent to obtain the first mixed reagent; wherein, the first dose is the sum of the first ratio dose and the temperature control dose, and the first ratio dose and the second dose satisfy the ratio.

[0074] At this point, step S30 includes:

[0075] S301. The remaining doses of the first reagent and the remaining doses of the second reagent are added to the first mixed reagent in batches to obtain the target mixed reagent.

[0076] In related technologies, samples and reagents are often added to the reaction vessel at once, although the order of addition may vary depending on the function. Since the number of times the reagents to be mixed differs in different technical solutions, the total number of times the reagents to be mixed is added is N, where N satisfies: 2 ≤ N ≤ 5.

[0077] Since the temperature-controlled dosage is only used for adjustment and not as a basic reagent unit, its specific amount cannot be too large. Therefore, in one embodiment, the temperature-controlled dosage satisfies a preset ratio relative to the first ratio dosage, and the preset ratio is P, wherein P satisfies: 0 < P ≤ 1 / 2. Specifically, P satisfies: 0 < P ≤ 1 / 3. Further, in one embodiment, P is 1 / 5.

[0078] In this embodiment, we take two reagents to be mixed and the total number of times the reagents to be mixed is added as an example. Of course, it is not limited to two reagents to be mixed and the total number of times the reagents to be mixed is added as two.

[0079] The two reagents to be mixed are reagent A and reagent B. The total amount of reagent A required is Va, and the total amount of reagent B required is Vb. Since the reagents to be mixed are added twice, they need to be added in two separate additions.

[0080] (1) When adding the first reagent A for the first time, the dosage of the first reagent A is V1a, which is determined according to the following formula:

[0081] V1a = Va / 2 + δ;

[0082] Wherein, δ is the temperature-controlled dosage, and V1a is the dosage of the first reagent A added for the first time, which is also the first ratio dosage. The range of δ can be within 0 to 1 / 3 of the first ratio dosage. In this embodiment, δ is taken as 1 / 5 of V1a.

[0083] The dosage of the second reagent B, i.e., the second dosage, is V1b, which is determined according to the following formula:

[0084] V1b = Vb / 2;

[0085] It is easy to see that since Va and Vb satisfy the ratio, Vb / 2 and Va / 2 also satisfy the ratio.

[0086] (2) When adding for the second time, the dosage of the first reagent A is V2a, which is determined according to the following formula:

[0087] V2a=Va / 2-δ;

[0088] The dosage of reagent B added is V2b, which is determined according to the following formula:

[0089] V2b = Vb / 2;

[0090] As can be seen, this embodiment, by changing the dosage of reagents during the addition process, ensures that the temperature of the mixture is slightly higher when the first and second reagents are added for the first time. This promotes a rapid reaction. Furthermore, because the amount of the temperature-controlling reagent (first reagent) added in the first addition is slightly larger, the excess heat is absorbed by the reaction vessel, effectively heating it quickly. Then, when the first and second reagents are added for the second time, since the reaction vessel has already been preheated by the temperature-controlling reagent added in the first addition, the slightly lower ratio of first reagent A and second reagent B does not cause a significant change in the temperature of the mixture and does not affect the reaction. The specific dosages of the first and second reagents to be added in the first and second additions need to be adjusted based on experiments. In summary, the slightly higher temperature of the first mixed reagent promotes the reaction, and although the amount of reagent added in the second addition is smaller, the reaction vessel has already been heated, so it does not affect the reaction.

[0091] In this embodiment, by controlling the dosage of reagents during the addition process, adding an appropriate amount of the first reagent during the first addition helps to promote the reaction, and deducting the amount of the first reagent added during the second addition ensures that the total amount of reagents used remains unchanged and does not alter the reaction system, making it more economical and practical.

[0092] As an example, step S203 specifically includes:

[0093] S2031. According to the first ratio dosage, the remaining dose of the first reagent is added to the first mixed reagent in N portions to obtain the target mixed reagent; wherein the dose added in one portion is the difference between the first ratio dosage and the temperature-controlled dosage;

[0094] S2032. According to the second dose, the remaining dose of the second reagent is divided into N portions and added to the first mixed reagent on an average basis to obtain the target mixed reagent; where N is a positive integer greater than or equal to 1.

[0095] It is important to understand that the remaining doses of the first and second reagents can be added to the reaction vessel at least once, wherein the amount of the first reagent added each time can be the first ratio dose. Correspondingly, the amount of the second reagent added each time is the second dose, to ensure that the amounts of the first and second reagents added each time meet the ratio.

[0096] It is worth mentioning that, since extra temperature control reagent was added to the mixture when the first reagent was added for the first time, the extra temperature control reagent added during the second, third, or nth addition of the first reagent needs to be deducted to ensure that the total amount of reagents used to be mixed remains unchanged and the reaction system is not altered.

[0097] The other second reagents can be divided into N equal additions based on the total number of additions of the reagents to be mixed.

[0098] When the total number of additions of the reagent to be mixed is 2, when adding the reagent to be mixed for the first time, add an appropriate amount of temperature control reagent, and then deduct the extra temperature control reagent added for the second time. After adding the first reagent twice, the addition of the first reagent is completed.

[0099] However, further, in this embodiment, the total number of times the reagent to be mixed is added is T, where T satisfies: 2 ≤ T ≤ 5. If the total number of times the reagent to be mixed is added is greater than 2, the specific values ​​of the first ratio dose and the second dose can be obtained by averaging the total dose of the first dose and the second dose with the total number of additions. That is, the second dose of the second reagent is added each time, while the first reagent is also added at the first ratio dose, except for the first time when a larger dose of temperature control is added and a subsequent time when a smaller dose of temperature control is added.

[0100] In this embodiment, by adding reagents in multiple batches on average, when the first reagent is added for the first time, an extra temperature control reagent is added to the mixture. Therefore, when adding the first reagent for the second, third, or nth time, the extra temperature control reagent added in the first time needs to be deducted to ensure that the total amount of reagents used remains unchanged, thus ensuring that the total amount of reagents used does not change the reaction system and is conducive to ensuring the normal progress of the reaction.

[0101] The above are merely preferred embodiments of this application and do not limit the patent scope of this application. Any equivalent structural or procedural transformations made using the content of this application's specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this application.

Claims

1. A method for preparing a mixed reagent, characterized in that, The method includes: Based on the mixing ratio of the target mixed reagents, at least two reagents to be mixed are provided, including a temperature-controlled reagent; A first mixed reagent is obtained by mixing a first dose of temperature-controlled reagent with a specified dose of other component reagents; wherein, the first dose is the sum of the first specified dose and the temperature-controlled dose, and the first specified dose and the specified dose satisfy the specified ratio. The remaining dose of temperature-controlled reagent and the remaining dose of each of the other component reagents are added to the first mixed reagent in batches to obtain the target mixed reagent.

2. The method for preparing the mixed reagent according to claim 1, characterized in that, The first mixed reagent is obtained by mixing the first dose of the temperature-controlled reagent with the other component reagents in the specified dosage, comprising: A first dose of temperature-controlled reagent and a second dose of second reagent are mixed to obtain the first mixed reagent; wherein, the first dose is the sum of the first ratio dose and the temperature-controlled dose, and the first ratio dose and the second dose satisfy the ratio described above; The step of adding the remaining dose of temperature-controlled reagent and the remaining dose of each of the other component reagents to the first mixed reagent in batches to obtain the target mixed reagent includes: The remaining dose of temperature-controlled reagent and the remaining dose of second reagent are added to the first mixed reagent in batches to obtain the target mixed reagent.

3. The method for preparing the mixed reagent according to claim 2, characterized in that, The step of adding the remaining dose of the temperature-controlled reagent and the remaining dose of the second reagent to the first mixed reagent in batches to obtain the target mixed reagent includes: According to the first ratio dosage, the remaining dose of temperature-controlled reagent is added to the first mixed reagent in N portions to obtain the target mixed reagent; wherein the dose added in one portion is the difference between the first ratio dosage and the temperature-controlled dosage; According to the second dose, the remaining dose of the second reagent is divided into N portions and added to the first mixed reagent on an average basis to obtain the target mixed reagent; where N is a positive integer greater than or equal to 1.

4. The method for preparing the mixed reagent according to any one of claims 1 to 3, characterized in that, The total number of times the reagent to be mixed is added is T, where T satisfies: 2≤T≤5.

5. The method for preparing the mixed reagent according to claim 4, characterized in that, The temperature-controlled dose satisfies a preset ratio relative to the first ratio dose, and the preset ratio is P, wherein P satisfies: 0 < P ≤ 1 / 2.

6. The method for preparing the mixed reagent according to claim 5, characterized in that, Specifically, P satisfies: 0 < P ≤ 1 / 3.

7. The method for preparing the mixed reagent according to claim 5, characterized in that, The value of P is 1 / 5.

8. A mixed reagent preparation system, characterized in that, The system includes: Reaction tank; At least two reagent addition branches are connected to the reaction vessel. The reagent addition branches correspond one-to-one with the quantity of the reagents to be mixed in the reaction vessel. One of the reagent addition branches is a temperature-controlled reagent addition branch. A controller is configured to, upon the first addition, control the temperature-controlled reagent addition branch to add a first dose of temperature-controlled reagent to the reaction tank, and control the other reagent addition branches to add the other component reagents in the specified dosage to the reaction tank, thereby obtaining a first mixed reagent; wherein, the first dose is the sum of the first specified dosage and the temperature-controlled dosage, and the first specified dosage and the specified dosage satisfy the ratio of the target mixed reagent; The controller is also used to control the temperature-controlled reagent addition branch to add the remaining dose of temperature-controlled reagent to the reaction tank in batches, and to simultaneously control the other reagent addition branches to add the remaining doses of other component reagents to the reaction tank to obtain the target mixed reagent.

9. The mixed reagent preparation system according to claim 8, characterized in that, The at least two reagent addition branches include: The first reagent addition branch is connected to the reaction cell and is used to add a temperature-controlled reagent to the reaction cell. The second reagent addition branch is connected to the reaction cell and is used to add a second reagent to the reaction cell. The controller is specifically used to control the first reagent adding branch to add a first dose of temperature-controlled reagent to the reaction cell, and to control the second reagent adding branch to add a second dose of second reagent to the reaction cell, so that the first dose of temperature-controlled reagent and the second dose of second reagent are mixed to obtain a first mixed reagent; The controller is specifically used to control the first reagent addition branch and the second reagent addition branch to add the remaining dose of temperature-controlled reagent and the remaining dose of second reagent to the reaction tank in batches to obtain the target mixed reagent, wherein the first dose is the sum of the first ratio dose and the temperature-controlled dose, and the first ratio dose and the second dose satisfy the ratio.

10. The mixed reagent preparation system according to claim 9, characterized in that, The first reagent addition branch includes a first negative pressure extractor; The second reagent addition branch includes a second negative pressure extractor.

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