A reagent preparation apparatus, a sample analysis system, and a reagent preparation method

Abstract

A reagent preparation device, a sample analysis system and a reagent preparation method, obtain the concentration detection result of the reagent actually prepared for preparing the reagent with the target concentration last time, calculate the quantitative parameter of the reagent preparation this time according to at least the concentration detection result of the last time, measure the first liquid and the second liquid according to the quantitative parameter of the reagent preparation this time, so as to prepare the reagent with the target concentration, thereby removing the influence of the aging deformation of the quantitative system, the reagent batch and the like on the concentration of the prepared reagent.

Description

Technical Field

[0001] This invention relates to the field of sample analysis, and more particularly to a reagent preparation apparatus, a sample analysis system, and a reagent preparation method. Background Technology

[0002] The application of solution reduction devices is becoming increasingly widespread. For example, in the medical device industry, the diluents for blood cell analyzers and the cleaning solutions for immunoassay instruments are used in large quantities, resulting in large container volumes and frequent replacements. This increases the workload for doctors and reduces the user experience. Therefore, designing an instrument to reduce concentrated solutions to normal concentrations significantly reduces the frequency of solution replacements and improves the doctor's experience.

[0003] The input to a solution reduction device typically includes pure water and a concentrated solution. Internally, it generally includes a quantitative system, a mixing system, and a concentration detection system. The quantitative system measures a specific volume of pure water and concentrated solution, with the volume ratio of pure water to concentrated solution being approximately constant. The mixing system mixes the measured amount of pure water and concentrated solution to create a homogeneous solution. The concentration detection system checks whether the concentration of the mixed solution meets the instrument's requirements; if not, it issues an alarm.

[0004] In actual use, it is often found that the solutions prepared and reduced by the reduction equipment do not meet the requirements, which greatly affects the use. Summary of the Invention

[0005] To address the above problems, this invention discloses a reagent preparation apparatus, a sample analysis system, and a reagent preparation method, which are described in detail below.

[0006] According to a first aspect, one embodiment provides a reagent preparation apparatus, comprising:

[0007] A metering component for measuring a first liquid and a second liquid according to metering parameters; the metering parameters are used to characterize the amount of the first liquid and / or the second liquid that needs to be measured.

[0008] A mixing component for mixing the first and second liquids measured by the metering component to prepare a reagent with a target concentration;

[0009] A concentration detection component for detecting the concentration of the reagent prepared by the mixing component; and

[0010] Temperature sensing components are used to detect temperature;

[0011] The processor is used in the preparation of this reagent:

[0012] Obtain the concentration detection result of the concentration detection component for the previously prepared reagent;

[0013] The temperature detected by the temperature sensing component during this reagent preparation is obtained;

[0014] Based on the concentration detection results of the previously prepared reagent and the temperature during the preparation of the current reagent, calculate the quantitative parameters for this reagent preparation.

[0015] The quantitative component controls the measurement of the first liquid and the second liquid according to the quantitative parameters of this reagent preparation, so that the mixing component can mix them to prepare the reagent with the target concentration.

[0016] In one embodiment, the processor calculates the quantitative parameters for this reagent preparation, including:

[0017] When the concentration detection result of the previously prepared reagent does not match the target concentration, the processor adjusts the quantitative parameters of the previous reagent preparation based on the deviation between the concentration detection result of the previous prepared reagent and the target concentration, and the temperature during the current reagent preparation, to obtain the quantitative parameters for the current reagent preparation; or,

[0018] When the concentration detection result of the previously prepared reagent matches the target concentration, the processor adjusts the quantitative parameters of the previous reagent preparation according to the temperature during the current reagent preparation to obtain the quantitative parameters of the current reagent preparation.

[0019] In one embodiment, the quantitative parameter includes at least one of the following:

[0020] The ratio of the amount of the first liquid to the amount of the second liquid;

[0021] The amount of the first liquid includes: the amount of the first liquid in the current preparation of the reagent, the change of the first liquid in the current preparation of the reagent compared to the previous one, or the change of the first liquid in the current preparation of the reagent compared to the default amount.

[0022] The amount of the second liquid includes: the amount of the second liquid in this preparation of the reagent, the change of the second liquid in this preparation of the reagent compared to the previous one, or the change of the second liquid in this preparation of the reagent compared to the default amount.

[0023] In one embodiment, the metering component includes at least a first metering container, a first infusion line, and a first drain line. The first metering container is used to measure the first liquid and / or the second liquid. The first infusion line is connected to the first metering container to deliver the liquid to be measured to the first metering container. The first drain line is connected between the first metering container and the mixing component to discharge the liquid measured by the first metering container to the mixing component.

[0024] The quantitative component also includes a liquid level detection component; the liquid detection component can detect its distance from the liquid surface; the processor calculates the target height of the liquid surface based on the quantitative parameters of this reagent preparation, which characterize the amount of the first liquid and / or the second liquid that needs to be measured; when the liquid detection component detects that the liquid enters the first quantitative container from the first infusion line and the liquid surface reaches the target height, the processor controls the quantitative component to stop supplying liquid to the first quantitative container.

[0025] or,

[0026] The quantitative component further includes a first liquid level detection component and a second liquid level detection component; the first liquid level detection component is used to detect whether the liquid level in the first quantitative container has reached a first liquid level, and the second liquid level detection component is used to detect whether the liquid level in the first quantitative container has reached a second liquid level, wherein the second liquid level is higher than the first liquid level; the processor can calculate the flow rate of the liquid entering the first quantitative container from the first infusion line based on the volume change and time when the liquid rises from the first liquid level to the second liquid level; the processor also calculates a target time based on the calculated flow rate and the quantitative parameters of this reagent preparation, and when the target time is reached, the processor controls the quantitative component to stop supplying liquid to the first quantitative container;

[0027] or,

[0028] The quantitative component also includes a flow sensor, which measures the flow rate of liquid entering the first quantitative container from the first infusion line. The processor calculates the target time based on the measured flow rate and the quantitative parameters of the reagent preparation. When the target time is reached, the processor controls the quantitative component to stop supplying liquid to the first quantitative container.

[0029] In one embodiment, the first metering container includes a first body and a first spout, the first spout being located above the first body and communicating with the first body, and the inner diameter of the first body being larger than the inner diameter of the first spout.

[0030] If the metering component includes the liquid level detection component, then the liquid level detection component is disposed at the first spout.

[0031] If the metering component includes a first liquid level detection component and a second liquid level detection component, then the first liquid level detection component and the second liquid level detection component are disposed at the first pool nozzle.

[0032] In one embodiment, the temperature sensing component includes an ambient temperature sensing element for detecting ambient temperature and / or a liquid temperature sensing element for detecting liquid temperature; the temperature detected by the temperature sensing component includes the ambient temperature and / or the liquid temperature.

[0033] In one embodiment, the liquid temperature sensing component is disposed in the first infusion line.

[0034] In one embodiment, the amount of the first liquid is the volume of the first liquid, and the amount of the second liquid is the volume of the second liquid.

[0035] In one embodiment, the first liquid is the stock solution of the reagent, and the second liquid is a diluent for diluting the stock solution of the reagent.

[0036] According to a second aspect, one embodiment provides a reagent preparation apparatus, comprising:

[0037] A metering component for measuring a first liquid and a second liquid according to metering parameters; the metering parameters are used to characterize the amount of the first liquid and / or the second liquid that needs to be measured.

[0038] A mixing component for mixing the first and second liquids measured by the metering component to prepare a reagent;

[0039] A concentration detection component for detecting the concentration of the reagent prepared by the mixing component; and,

[0040] The processor is configured to calculate the quantitative parameters for the current reagent preparation based at least on the detection results of the concentration detection component on the reagent previously prepared by the mixing component; the processor controls the quantitative component to measure the first liquid and the second liquid according to the quantitative parameters for the current reagent preparation, so that the mixing component can mix them to prepare the reagent with the target concentration.

[0041] According to a third aspect, one embodiment provides a sample analysis system, comprising:

[0042] Multiple sample analysis modules, which are used to measure samples;

[0043] Tracks are used to connect the various sample analysis modules;

[0044] The scheduling device is used to schedule samples transported via the track to the corresponding sample analysis modules;

[0045] The reagent preparation apparatus described in any embodiment of this document is used to supply reagents to the sample analysis module.

[0046] According to the fourth aspect, one embodiment provides a method for preparing a reagent, comprising:

[0047] Obtain the concentration detection result of the reagent actually prepared in the previous preparation of the reagent with the target concentration, wherein the reagent is prepared from a first liquid and a second liquid;

[0048] Obtain the temperature at which the reagent was prepared;

[0049] Based on the previous concentration detection results and the temperature during the preparation of the reagent, the quantitative parameters for this reagent preparation are calculated; the quantitative parameters are used to characterize the amount of the first liquid and / or the second liquid that needs to be measured.

[0050] Based on the quantitative parameters for this reagent preparation, the first liquid and the second liquid were measured to prepare a reagent with the target concentration.

[0051] In one embodiment, calculating the quantitative parameters for this reagent preparation includes:

[0052] The ratio of the amount of the first liquid to the amount of the second liquid.

[0053] The amount of the first liquid includes: the amount of the first liquid in the current preparation of the reagent, the change of the first liquid in the current preparation of the reagent compared to the previous one, or the change of the first liquid in the current preparation of the reagent compared to the default amount.

[0054] The amount of the second liquid includes: the amount of the second liquid in this preparation of the reagent, the change of the second liquid in this preparation of the reagent compared to the previous one, or the change of the second liquid in this preparation of the reagent compared to the default amount.

[0055] In one embodiment, the temperature includes ambient temperature, a first liquid temperature, and / or a second liquid temperature.

[0056] In one embodiment, the first liquid is the stock solution of the reagent, and the second liquid is a diluent for diluting the stock solution of the reagent.

[0057] According to a fifth aspect, one embodiment provides a method for preparing a reagent, comprising:

[0058] Obtain the concentration detection result of the reagent actually prepared in the previous preparation of the reagent with the target concentration, wherein the reagent is prepared from a first liquid and a second liquid;

[0059] Calculate the quantitative parameters for this reagent preparation based at least on the previous concentration detection results;

[0060] Based on the quantitative parameters for this reagent preparation, the first liquid and the second liquid were measured to prepare a reagent with the target concentration.

[0061] Based on the reagent preparation apparatus, sample analysis system, and reagent preparation method of the above embodiments, the concentration detection result of the reagent actually prepared in the previous preparation of a reagent with a target concentration is obtained, and the quantitative parameters of the current reagent preparation are calculated based at least on the previous concentration detection result, thereby eliminating the influence of aging and deformation of the quantitative system, reagent batch, etc. on the concentration of the prepared reagent. Attached Figure Description

[0062] Figure 1 This is a schematic diagram of the structure of a reagent preparation apparatus according to one embodiment;

[0063] Figure 2 This is a schematic diagram of the structure of a reagent preparation apparatus according to one embodiment;

[0064] Figure 3 This is a schematic diagram of the structure of a quantitative component according to one embodiment;

[0065] Figures 4(a), 4(b), and 4(c) are schematic diagrams of the quantitative components in three embodiments;

[0066] Figure 5 This is a schematic diagram of the structure of a quantitative component according to one embodiment;

[0067] Figure 6 This is a schematic diagram of the structure of a sample analysis system according to one embodiment;

[0068] Figure 7 This is a flowchart of a reagent preparation method according to one embodiment;

[0069] Figure 8 This is a flowchart of a reagent preparation method according to one embodiment. Detailed Implementation

[0070] The present invention will now be described in further detail with reference to specific embodiments and accompanying drawings. Similar elements in different embodiments are referred to by associated similar element reference numerals. In the following embodiments, many details are described to facilitate a better understanding of this application. However, those skilled in the art will readily recognize that some features may be omitted in different situations, or may be replaced by other elements, materials, or methods. In some cases, certain operations related to this application are not shown or described in the specification. This is to avoid obscuring the core parts of this application with excessive description. For those skilled in the art, detailed description of these related operations is not necessary; they can fully understand the related operations based on the description in the specification and general technical knowledge in the art.

[0071] Furthermore, the features, operations, or characteristics described in the specification can be combined in any suitable manner to form various embodiments. At the same time, the steps or actions in the method description can be rearranged or adjusted in a manner obvious to those skilled in the art. Therefore, the various orders in the specification and drawings are only for the clear description of a particular embodiment and do not imply a necessary order, unless otherwise stated that a particular order must be followed.

[0072] The serial numbers assigned to components in this document, such as "first" and "second," are used only to distinguish the described objects and have no sequential or technical meaning. The terms "connection" and "linkage" used in this application, unless otherwise specified, include both direct and indirect connections (linkages).

[0073] The quantitative determination of liquids is crucial in the reduction process of concentrated reagents. The accuracy of the quantitative determination directly determines whether the concentration of the reduced solution can meet the requirements. The applicant found that the quantitative structure often exhibits instability, and its quantitative volume frequently changes, thus affecting the concentration of the source solution. Further research revealed that this is caused by several factors, such as the aging and deformation of the quantitative structure itself, as well as factors such as solution temperature and ambient temperature.

[0074] Based on the above findings, this invention designs a solution reduction device and a precision quantitative technology based on feedback control. In some embodiments, the volume of pure water or concentrated solution quantified by the quantitative component is changed by detecting the solution concentration value by the concentration detection component, thereby eliminating the influence of aging and deformation of the quantitative system, reagent batches, etc. on the concentration of the reduced solution. In some embodiments, a precision quantitative device is designed to adjust the volume of the quantitative solution by detecting ambient temperature and solution temperature, thereby eliminating the influence of ambient temperature and solution temperature on the concentration of the reduced solution.

[0075] Please refer to Figure 1 In some embodiments, the reagent preparation apparatus includes a quantification component 10, a mixing component 30, a concentration detection component 50, and a processor 60; please refer to Figure 2 In some embodiments, the reagent preparation apparatus also includes a temperature sensing component 70, which will be described in detail below.

[0076] The metering component 10 is used to measure a first liquid and a second liquid according to metering parameters; the metering parameters characterize the amount of the first liquid and / or the second liquid to be measured. In some embodiments, the amount of the first liquid is the volume of the first liquid, and the amount of the second liquid is the volume of the second liquid. In some embodiments, the first liquid is a stock solution of the reagent; in some embodiments, the second liquid is a dilution of the stock solution of the diluent. The mixing component 30 is used to mix the first liquid and the second liquid measured by the metering component 10 to prepare a reagent with a target concentration; the concentration detection component 50 is used to detect the concentration of the reagent prepared by the mixing component 30. The reagent preparation apparatus can prepare a reagent with a target concentration by performing operations such as metering, mixing, and concentration detection on the liquid, for example, by diluting the stock solution of the reagent to the target concentration.

[0077] Please refer to Figure 3 In some embodiments, the metering component 10 includes a first metering container 11, a first infusion line 11a, and a first drain line 11b. The first metering container 11 is used to measure the first liquid and / or the second liquid. The first infusion line 11a is connected to the first metering container 11 to deliver the liquid to be measured to the first metering container 11. The first drain line 11b is connected between the first metering container 11 and the mixing component 30 to discharge the liquid measured by the first metering container 11 to the mixing component 30. For example, the first metering container 11 is only used to measure the first liquid. Specifically, the first liquid is delivered to the first metering container 11 through the first infusion line 11a, the first metering container 11 is used to measure the first liquid quantitatively, and then the measured first liquid is discharged to the mixing component 30 through the first drain line 11b. For example, the first metering container 11 is used only to measure the second liquid. Specifically, the second liquid is delivered to the first metering container 11 through the first infusion line 11a. The first metering container 11 is used to measure the second liquid in a quantitative manner, and then the measured second liquid is discharged to the mixing component 30 through the first drainage line 11b. For example, the first metering container 11 can measure the first liquid and the second liquid in stages. For example, the first liquid can be measured first, and then the measured first liquid can be discharged to the mixing component 30, then the second liquid can be measured, and then the measured second liquid can be discharged to the mixing component 30. Alternatively, the second liquid can be measured first, and then the measured second liquid can be discharged to the mixing component 30, then the first liquid can be measured, and then the measured first liquid can be discharged to the mixing component 30. In such an example, the first liquid and the second liquid are measured using the same first metering container 11.

[0078] The quantitative component 10 of this application has the function of changing the quantitative volume, and several implementation examples are given below.

[0079] In some embodiments, the first metering container 11 includes a first body 11c and a first nozzle 11d. The first nozzle 11d is located above and communicates with the first body 11c, and the inner diameter of the first body 11c is larger than the inner diameter of the first nozzle 11d. This shape of the first metering container 11, which is narrower at the top and wider at the bottom, makes fluctuations in the liquid level have a smaller impact on the percentage of the metered volume. In other embodiments, the first metering container 11 may not be of this shape; it can be of any shape as long as it can achieve its metering function.

[0080] Referring to Figure 4(a), in some embodiments, the metering component 10 further includes a liquid level detection component 12; the liquid detection component 12 is capable of detecting its distance from the liquid surface. In some embodiments, the liquid detection component 12 can be implemented using a capacitive sensor, an optical coupler detector, an ultrasonic detector, etc. In some embodiments, the liquid detection component 12 is provided with a first spout 11d of the first metering container 11. By detecting the distance between the liquid detection component 12 and the liquid surface, different distances correspond to different amounts of liquid, thus enabling the metering component 10 to quantify the liquid. For example, the processor 60 calculates the target height of the liquid surface based on the quantitative parameters of this reagent preparation, which characterize the amount of the first liquid and / or the second liquid to be measured. When the liquid detection component 12 detects that liquid has entered the first metering container 11 from the first infusion line 11a and the liquid surface has reached the target height, the processor 60 controls the metering component 10 to stop supplying liquid to the first metering container 11.

[0081] Referring to Figure 4(b), in some embodiments, the metering component 10 further includes a first liquid level detection component 13 and a second liquid level detection component 14. The first liquid level detection component 13 is used to detect whether the liquid level in the first metering container 11 has reached a first liquid level, and the second liquid level detection component 14 is used to detect whether the liquid level in the first metering container 11 has reached a second liquid level, wherein the second liquid level is higher than the first liquid level. In some embodiments, the first liquid level detection component 13 can be implemented by a capacitive sensor, an optocoupler detector, an ultrasonic detector, etc. In some embodiments, the second liquid level detection component 14 can be implemented by a capacitive sensor, an optocoupler detector, an ultrasonic detector, etc. In some embodiments, the first liquid level detection component 13 and the second liquid level detection component 14 are disposed at the first spout 11d of the first metering container 11. Through the first liquid level detection component 13 and the second liquid level detection component 14, the metering component 10 can realize the metering of liquid. For example, when the liquid reaches the first liquid level, it corresponds to one volume of liquid. When the liquid reaches the second liquid level, it corresponds to another volume of liquid. The time it takes for the liquid to rise from the first liquid level to the second liquid level can also be obtained, thus obtaining the flow rate. Based on this information, the metering of liquid can be completed. For example, the processor 60 can calculate the flow rate of liquid entering the first metering container 11 from the first infusion pipeline based on the volume change and time when the liquid rises from the first liquid level to the second liquid level. The processor 60 also calculates the target time based on the calculated flow rate and the metering parameters of this reagent preparation. When the target time is reached, the processor 60 controls the metering component 10 to stop supplying liquid to the first metering container 11.

[0082] Referring to Figure 4(c), in some embodiments, the quantitative component 10 further includes a flow sensor 15, which is used to measure the flow rate of liquid entering the first quantitative container 11 from the first infusion line 11a. The processor 60 calculates the target time based on the measured flow rate and the quantitative parameter amount of the reagent preparation. When the target time is reached, the processor 60 controls the quantitative component 10 to stop supplying liquid to the first quantitative container 11.

[0083] In some examples, the metering component 10 may consist of only a first metering container 11, which, as described above, can measure the first liquid and the second liquid in a time-sharing manner—for example… Figure 3 This is one example. In other examples, the metering component 10 may include multiple first metering containers 11, such as two, one first metering container 11 for measuring a first liquid and one first metering container 11 for measuring a second liquid—for example… Figure 5 This is one example. In some examples, Figure 5The two first quantitative containers 11 contained therein can have the same structure, but their size can be designed according to the requirements. For example, they can be designed as two first quantitative containers 11 of different sizes. Both of these first quantitative containers 11 can be equipped with liquid level detection components 12, or they can be equipped with first liquid level detection components 13 and second liquid level detection components 14, or they can be equipped with flow sensors 15. Figure 5 The metering component 10 shows two metering containers, one for measuring a first liquid and the other for measuring a second liquid. The two metering containers have the same structure. Understandably, in some examples, the metering component 10 includes two metering containers, one for measuring the first liquid and the other for measuring the second liquid. The shapes and structures of these two metering containers may also be different, as long as they can respectively perform the functions of measuring the first liquid and measuring the second liquid.

[0084] Temperature sensing component 70 is used to detect temperature. In some embodiments, temperature sensing component 70 includes an ambient temperature sensing element for detecting ambient temperature and / or a liquid temperature sensing element for detecting liquid temperature; the temperature detected by the temperature sensing component includes ambient temperature and / or liquid temperature, such as the temperature of a first liquid measured by a metering component and / or the temperature of a second liquid measured by a metering component. In some embodiments, the liquid temperature sensing element is disposed in the first infusion line 11a. Figure 5 In the example shown, liquid temperature sensing components can be installed in the first infusion lines 11a of both first metering containers 11.

[0085] In some examples, the process of solution reduction technology based on feedback control is as follows: First, the reagent preparation device performs a reduction process of concentrated reagent through the metering component 10 and the mixing component 30. The concentration detection component 50 detects the concentration of the reduced solution and compares this concentration with the target concentration to determine the change in the metering volume of the first liquid, such as concentrated solution, and / or the second liquid, such as pure water (this can be a change in the metering volume of one liquid or both liquids). When the reagent preparation device performs the next metering of pure water or concentrated reagent, its metering volume is changed according to the calculated amount, thereby changing the concentration of the reduced solution, i.e., the prepared reagent, and achieving closed-loop feedback control. In this way, the influence of factors such as aging of the metering component and reagent batches on the concentration of the prepared reagent can be eliminated.

[0086] In some embodiments, the processor 50 is used to calculate the quantitative parameters for the current reagent preparation based at least on the detection results of the concentration detection unit 50 on the reagent previously prepared by the mixing unit 30; the processor 50 controls the quantitative unit 10 to measure the first liquid and the second liquid according to the quantitative parameters for the current reagent preparation, so that the mixing unit 30 can mix them to prepare the reagent with the target concentration.

[0087] In some embodiments, if the concentration detection result of the previously prepared reagent does not match the target concentration or is mismatched with the target concentration, the processor 50 adjusts the quantitative parameters of the previous reagent preparation based on the deviation between the concentration detection result of the previously prepared reagent and the target concentration, and obtains the quantitative parameters of the current reagent preparation.

[0088] In some embodiments, when the concentration detection result of the previously prepared reagent matches the target concentration, the processor 50 uses the quantitative parameters of the previous reagent preparation as the quantitative parameters of the current reagent preparation.

[0089] In some embodiments, the effect of temperature can also be considered. Temperature affects the volume of the quantitative liquid, including the effect of ambient temperature on the metering component 10 and the effect of the liquid's own temperature on the amount of liquid. In some specific embodiments, during the preparation of the reagent, the processor 50: acquires the concentration detection result of the previously prepared reagent from the concentration detection component 50; acquires the temperature detected by the temperature sensing component 70 during the preparation of the reagent; calculates the quantitative parameters for the current reagent preparation based on the concentration detection result of the previously prepared reagent and the temperature during the current reagent preparation; and controls the metering component 10 to measure the first and second liquids according to the quantitative parameters for the current reagent preparation, so that the mixing component 30 can mix them to prepare a reagent with the target concentration. The effect of temperature on the quantitative parameters can be a function or a mapping table, etc.

[0090] In some embodiments, if the concentration detection result of the previously prepared reagent does not match the target concentration or is mismatched with the target concentration, the processor 50 adjusts the quantitative parameters of the previous reagent preparation based on the deviation between the concentration detection result of the previously prepared reagent and the target concentration, and the temperature during the current reagent preparation, to obtain the quantitative parameters of the current reagent preparation.

[0091] In some embodiments, when the concentration detection result of the previously prepared reagent matches the target concentration, the processor 50 adjusts the quantitative parameters of the previous reagent preparation based on the temperature during the current reagent preparation to obtain the quantitative parameters for the current reagent preparation.

[0092] In this article, the concept of whether the concentration detection result matches or does not match the target concentration is as follows: The target concentration can be a single value. Matching the concentration detection result with the target concentration means that the concentration detection result is within a certain range of the target concentration value, i.e., the difference between the two is within a preset range. Conversely, it is considered a mismatch. Alternatively, the target concentration itself can be a range value. Matching the concentration detection result with the target concentration means that the concentration detection result is within this range of the target concentration. Conversely, it is considered a mismatch.

[0093] In some embodiments, the quantitative parameter includes at least one of the following:

[0094] The ratio of the amount of the first liquid to the amount of the second liquid;

[0095] The amount of the first liquid includes: the amount of the first liquid in the current preparation of the reagent, the change of the first liquid in the current preparation of the reagent compared to the previous one, or the change of the first liquid in the current preparation of the reagent compared to the default amount.

[0096] The amount of the second liquid includes: the amount of the second liquid in this preparation of the reagent, the change of the second liquid in this preparation of the reagent compared to the previous one, or the change of the second liquid in this preparation of the reagent compared to the default amount.

[0097] The default amount mentioned above can be a pre-set amount at the factory, which can be the amount needed to prepare the reagent at the target concentration under normal temperature conditions.

[0098] Let's take the example of the metering component 10 also including a first liquid level detection component 13 and a second liquid level detection component 14 to provide a complete explanation:

[0099] (1) Before leaving the factory, the liquid volume corresponding to the first liquid level and the second liquid level can be obtained. For example, before leaving the factory, the manufacturer slowly injects the solution into the first quantitative container 11. When the first liquid level detection component 13 detects the solution, the addition of solution to the container is stopped, the solution is discharged, and the volume of the solution is measured and recorded as V1. Then, the solution is slowly injected into the container again. When the second liquid level detection component 14 detects the solution, the addition of solution to the container is stopped, the solution is discharged, and the volume of the solution is measured and recorded as V2. The difference between the two solution volumes is recorded as V, that is, V = V1 - V2.

[0100] (2) Manufacturers can also determine the influence of ambient temperature and solution temperature on the quantitative volume of the container, and determine the relationship between ambient temperature, solution temperature and quantitative component volume; it can be a function or a mapping table;

[0101] (3) When the metering component 10 starts metering the solution, it first detects the ambient temperature and solution temperature to determine the magnitude of their influence on the metering volume of the precision metering device, such as the first metering container 11. Based on the adjustment amount of the metering volume of the first liquid and / or the second liquid during the previous reagent preparation, it calculates the change in the volume of the first liquid and / or the second liquid during this reagent preparation. Taking the change in the volume of only one liquid as an example, the change in volume during this reagent preparation is Vcomp. Then, the solution is injected, and the time T1 when the first liquid level detection component 13 detects the liquid surface and the time T2 when the second liquid level detection component 14 detects the liquid surface are recorded. By interpolating the two detection times and the volume deviation of the liquid surface from the two liquid level detection sensors, the speed D of the solution injection into the container is calculated using the following formula:

[0102] D = V / (T2-T1);

[0103] Based on the calculated quantitative volume Vcomplement of the compensation solution, determined by the ambient temperature and solution temperature, and the injection flow rate of the solution, the inflow time for the compensation solution is calculated using the following formula:

[0104] Tcomplement = Vcomplement / D

[0105] After the solution has been flowing in for a time T, the solution injection is stopped, thus completing the feedback control adjustment of the liquid's quantitative volume.

[0106] The above is a brief description of the reagent preparation device.

[0107] Some embodiments of the present invention also disclose a sample analysis system. Please refer to... Figure 6 Some embodiments of the sample analysis system include multiple sample analysis modules 80, a track 90, a scheduling device 91, and a reagent preparation device as described in any embodiment herein.

[0108] The sample analysis module 80 is used to measure samples; the track 90 is used to connect the sample analysis modules 80 together; the scheduling device 91 is used to schedule the samples transmitted through the track 90 to the corresponding sample analysis module 80; such a pipeline system can greatly improve the sample measurement speed; in this process, the reagent preparation device is used to supply reagents to the sample analysis module 80.

[0109] This invention also discloses a reagent preparation method, which is described in detail below.

[0110] Please refer to Figure 7 Some embodiments of the reagent preparation method include the following steps:

[0111] Step 100: Obtain the concentration detection result of the reagent actually prepared in the previous preparation of the reagent with the target concentration, wherein the reagent is prepared from the first liquid and the second liquid.

[0112] In some embodiments, the first liquid is the stock solution of the reagent, and the second liquid is a diluent of the stock solution of the reagent, such as pure water.

[0113] Step 110: Calculate the quantitative parameters for this reagent preparation based at least on the previous concentration test results.

[0114] In some embodiments, if the concentration detection result of the previously prepared reagent does not match the target concentration or does not match the target concentration, then step 110 adjusts the quantitative parameters of the previous reagent preparation based on the deviation between the concentration detection result of the previously prepared reagent and the target concentration to obtain the quantitative parameters of the current reagent preparation.

[0115] In some embodiments, when the concentration detection result of the previously prepared reagent matches the target concentration, step 110 uses the quantitative parameters of the previous reagent preparation as the quantitative parameters of the current reagent preparation.

[0116] Step 120: According to the quantitative parameters of this reagent preparation, measure the first liquid and the second liquid to prepare a reagent with the target concentration.

[0117] In some embodiments, the quantitative parameter includes at least one of the following:

[0118] The ratio of the amount of the first liquid to the amount of the second liquid;

[0119] The amount of the first liquid includes: the amount of the first liquid in the current preparation of the reagent, the change of the first liquid in the current preparation of the reagent compared to the previous one, or the change of the first liquid in the current preparation of the reagent compared to the default amount.

[0120] The amount of the second liquid includes: the amount of the second liquid in this preparation of the reagent, the change of the second liquid in this preparation of the reagent compared to the previous one, or the change of the second liquid in this preparation of the reagent compared to the default amount.

[0121] Please refer to Figure 8 Some embodiments of the reagent preparation method include the following steps:

[0122] Step 200: Obtain the concentration detection result of the reagent actually prepared in the previous preparation of the reagent with the target concentration, wherein the reagent is prepared from the first liquid and the second liquid.

[0123] In some embodiments, the first liquid is the stock solution of the reagent, and the second liquid is a diluent of the stock solution of the reagent, such as pure water.

[0124] Step 210: Obtain the temperature at which the reagent was prepared.

[0125] Temperature affects the volume of a metered liquid, including the effect of ambient temperature on the metering component 10 and the effect of the liquid's own temperature on the amount of liquid. Therefore, in some embodiments, the temperature obtained in step 210 includes the ambient temperature, a first liquid temperature, and / or a second liquid temperature.

[0126] Step 220: Based on the previous concentration test results and the temperature during this reagent preparation, calculate the quantitative parameters for this reagent preparation; the quantitative parameters are used to characterize the amount of the first liquid and / or the second liquid that needs to be measured.

[0127] In some embodiments, when the concentration detection result of the previously prepared reagent does not match the target concentration or does not match the target concentration, step 220 adjusts the quantitative parameters of the previous reagent preparation based on the deviation between the concentration detection result of the previously prepared reagent and the target concentration, and the temperature during the current reagent preparation, to obtain the quantitative parameters of the current reagent preparation.

[0128] In some embodiments, when the concentration detection result of the previously prepared reagent matches the target concentration, step 220 adjusts the quantitative parameters of the previous reagent preparation based on the temperature during the current reagent preparation to obtain the quantitative parameters of the current reagent preparation.

[0129] Step 230: According to the quantitative parameters of this reagent preparation, measure the first liquid and the second liquid to prepare a reagent with the target concentration.

[0130] In some embodiments, the quantitative parameter includes at least one of the following:

[0131] The ratio of the amount of the first liquid to the amount of the second liquid;

[0132] The amount of the first liquid includes: the amount of the first liquid in the current preparation of the reagent, the change of the first liquid in the current preparation of the reagent compared to the previous one, or the change of the first liquid in the current preparation of the reagent compared to the default amount.

[0133] The amount of the second liquid includes: the amount of the second liquid in this preparation of the reagent, the change of the second liquid in this preparation of the reagent compared to the previous one, or the change of the second liquid in this preparation of the reagent compared to the default amount.

[0134] This document describes various exemplary embodiments with reference to them. However, those skilled in the art will recognize that changes and modifications can be made to the exemplary embodiments without departing from the scope of this document. For example, various operational steps and components for performing operational steps can be implemented in different ways depending on the specific application or considering any number of cost functions associated with the operation of the system (e.g., one or more steps can be deleted, modified, or combined with other steps).

[0135] While the principles herein have been illustrated in various embodiments, numerous modifications to the structure, arrangement, proportions, elements, materials, and components, particularly suited to specific environmental and operational requirements, may be used without departing from the principles and scope of this disclosure. These modifications and other alterations or alterations will be included within the scope of this document.

[0136] The foregoing specific descriptions have been described with reference to various embodiments. However, those skilled in the art will recognize that various modifications and changes can be made without departing from the scope of this disclosure. Therefore, considerations for this disclosure are to be illustrative rather than restrictive, and all such modifications are to be included within its scope. Similarly, advantages, other advantages, and solutions to problems with respect to various embodiments have been described above. However, benefits, advantages, solutions to problems, and any elements that produce these, or make them more explicit, should not be construed as critical, essential, or necessary. The term “comprising” and any other variations thereof as used herein are non-exclusive inclusion, meaning that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not expressly listed or not part of the process, method, system, article, or apparatus. Furthermore, the term “coupled” and any other variations thereof as used herein refer to physical connections, electrical connections, magnetic connections, optical connections, communication connections, functional connections, and / or any other connections.

[0137] Those skilled in the art will recognize that many changes can be made to the details of the above embodiments without departing from the basic principles of the invention. Therefore, the scope of the invention should be determined only by the claims.

Claims

1. A reagent preparation device, characterized by, The application relates to a reagent preparation device and a reagent preparation method. The application comprises: a quantifying component for quantifying a first liquid and a second liquid according to a quantifying parameter; the quantifying parameter is used to represent the amount of the first liquid and the second liquid that needs to be quantified; a mixing component for mixing the first liquid and the second liquid quantified by the quantifying component to form a mixed liquid to prepare a reagent with a target concentration; a concentration detecting component for detecting the concentration of the reagent prepared by the mixing component; a temperature sensing component for detecting the temperature of the reagent during preparation; a processor for obtaining the concentration detection result of the reagent prepared last time by the concentration detecting component during the preparation of the reagent this time; obtaining the temperature of the reagent this time detected by the temperature sensing component; the temperature sensing component comprises an ambient temperature sensing component for detecting the ambient temperature and / or a liquid temperature sensing component for detecting the liquid temperature; the temperature detected by the temperature sensing component comprises the ambient temperature and / or the liquid temperature; the liquid temperature comprises the first liquid temperature and / or the second liquid temperature; calculating the quantifying parameter of the reagent this time according to the concentration detection result of the reagent prepared last time and the temperature of the reagent this time; controlling the quantifying component to quantify the first liquid and the second liquid according to the quantifying parameter of the reagent this time for the mixing component to prepare the reagent with the target concentration. The processor calculates the quantifying parameter of the reagent this time, which comprises:

2. The reagent preparation apparatus according to claim 1, wherein when the concentration detection result of the reagent prepared last time does not match the target concentration, the processor adjusts the quantifying parameter of the reagent prepared last time according to the deviation of the concentration detection result of the reagent prepared last time from the target concentration and the temperature of the reagent this time to obtain the quantifying parameter of the reagent this time; or when the concentration detection result of the reagent prepared last time matches the target concentration, the processor adjusts the quantifying parameter of the reagent prepared last time according to the temperature of the reagent this time to obtain the quantifying parameter of the reagent this time. The quantifying parameter comprises at least one of the following:

3. The reagent preparation apparatus according to claim 1 or 2, wherein the ratio of the amount of the first liquid and the amount of the second liquid; the amount of the first liquid, which comprises the amount of the first liquid during the preparation of the reagent this time, the change amount of the first liquid during the preparation of the reagent this time compared with last time or the change amount of the first liquid during the preparation of the reagent this time compared with the default amount; the amount of the second liquid, which comprises the amount of the second liquid during the preparation of the reagent this time, the change amount of the second liquid during the preparation of the reagent this time compared with last time or the change amount of the second liquid during the preparation of the reagent this time compared with the default amount. The quantifying component comprises at least a first quantifying container, a first liquid feeding pipeline and a first liquid discharging pipeline; the first quantifying container is used to quantify the first liquid and / or the second liquid; the first liquid feeding pipeline is connected with the first quantifying container and is used to feed the liquid to be quantified to the first quantifying container; and the first liquid discharging pipeline is connected between the first quantifying container and the mixing component and is used to discharge the liquid quantified by the first quantifying container to the mixing component.

4. The reagent preparation apparatus according to claim 1 or 2, wherein ​ The quantitative component further comprises a liquid level detection component; the liquid level detection component is capable of detecting the liquid level; The processor calculates the target height of the liquid level according to the amount of the first liquid and / or the second liquid to be taken as characterized by the quantitative parameters of the reagent preparation; when the liquid level detection component detects that the liquid enters the first quantitative container from the first liquid supply pipeline and the liquid level reaches the target height, the processor controls the quantitative component to stop supplying liquid to the first quantitative container; Alternatively, The quantitative component further comprises a first liquid level detection component and a second liquid level detection component; the first liquid level detection component is used to detect whether the liquid level in the first quantitative container reaches a first liquid level, and the second liquid level detection component is used to detect whether the liquid level in the first quantitative container reaches a second liquid level, wherein the second liquid level is higher than the first liquid level; the processor is capable of calculating the flow rate of the liquid entering the first quantitative container from the first liquid supply pipeline according to the volume change and time of the liquid rising from the first liquid level to the second liquid level; the processor further calculates the target time according to the calculated flow rate and the quantitative parameters of the reagent preparation; when the target time is reached, the processor controls the quantitative component to stop supplying liquid to the first quantitative container; Alternatively, The quantitative component further comprises a flow sensor for measuring the flow rate of the liquid entering the first quantitative container from the first liquid supply pipeline; The processor calculates the target time according to the measured flow rate and the quantitative parameters of the reagent preparation; when the target time is reached, the processor controls the quantitative component to stop supplying liquid to the first quantitative container.

5. The reagent preparation apparatus according to claim 4, wherein The first quantitative container comprises a first pool body and a first pool nozzle, the first pool nozzle is arranged above the first pool body and communicates with the first pool body, and the inner diameter of the first pool body is greater than that of the first pool nozzle; If the quantitative component comprises the liquid level detection component, the liquid level detection component is arranged in the first pool nozzle; If the quantitative component comprises the first liquid level detection component and the second liquid level detection component, the first liquid level detection component and the second liquid level detection component are arranged in the first pool nozzle.

6. The reagent preparation apparatus according to claim 4, wherein The liquid temperature sensing component is arranged in the first liquid supply pipeline.

7. The reagent preparation apparatus according to claim 1, wherein The amount of the first liquid is the volume of the first liquid, and the amount of the second liquid is the volume of the second liquid.

8. The reagent preparation apparatus according to claim 1, wherein The first liquid is the stock solution of the reagent, and the second liquid is the dilution solution for diluting the stock solution of the reagent.

9. A reagent preparation device, characterized by, Comprise: A quantitative component for taking the first liquid and the second liquid according to quantitative parameters; The quantitative parameters are used to characterize the amount of the first liquid and the second liquid to be taken; A mixing component for mixing the first liquid and the second liquid taken by the quantitative component to form a mixed liquid to prepare a reagent with a target concentration; A concentration detection component for detecting the concentration of the reagent prepared by the mixing component; And, The processor calculates the quantitative parameter of the present reagent preparation, including:

10. The reagent preparation apparatus according to claim 9, wherein When the concentration detection result of the reagent prepared last time does not match the target concentration, the processor adjusts the quantitative parameter of the reagent preparation last time according to at least the deviation of the concentration detection result of the reagent prepared last time from the target concentration, to obtain the quantitative parameter of the present reagent preparation; or, When the concentration detection result of the reagent prepared last time matches the target concentration, the processor takes the quantitative parameter of the reagent preparation last time as the quantitative parameter of the present reagent preparation. The quantitative parameter includes at least one of:

11. The reagent preparation device according to claim 9 or 10, characterized in that The ratio of the amount of the first liquid and the amount of the second liquid; The amount of the first liquid, including: the amount of the first liquid in the present reagent preparation, the change amount of the first liquid in the present reagent preparation compared with the last time, or the change amount of the first liquid in the present reagent preparation compared with the default amount; The amount of the second liquid, including: the amount of the second liquid in the present reagent preparation, the change amount of the second liquid in the present reagent preparation compared with the last time, or the change amount of the second liquid in the present reagent preparation compared with the default amount. The quantitative component includes at least a first quantitative container, a first liquid feeding pipeline and a first liquid discharging pipeline, the first quantitative container is used to measure the first liquid and / or the second liquid, the first liquid feeding pipeline is connected with the first quantitative container, to feed the liquid to be measured to the first quantitative container; the first liquid discharging pipeline is connected between the first quantitative container and the mixing component, to discharge the liquid measured by the first quantitative container to the mixing component; 12. The reagent preparation apparatus according to claim 9 or 10, wherein The quantitative component further includes a liquid level detection component; the liquid level detection component can detect the liquid surface height; The processor calculates the target height of the liquid surface according to the amount of the first liquid and / or the second liquid to be measured represented by the quantitative parameter of the present reagent preparation, when the liquid detection component detects that the liquid enters the first quantitative container from the first liquid feeding pipeline and the liquid surface reaches the target height, the processor controls the quantitative component to stop feeding liquid to the first quantitative container; Or, ​ The quantitative component further comprises a first liquid level detection component and a second liquid level detection component; the first liquid level detection component is used to detect whether the liquid level in the first quantitative container reaches a first liquid level, and the second liquid level detection component is used to detect whether the liquid level in the first quantitative container reaches a second liquid level, wherein the second liquid level is higher than the first liquid level; the processor can calculate the flow rate of the liquid entering the first quantitative container from the first infusion pipeline according to the volume change amount and the time when the liquid rises from the first liquid level to the second liquid level; the processor further calculates a target time according to the calculated flow rate and the quantitative parameters of the reagent preparation this time, and when the target time is reached, the processor controls the quantitative component to stop supplying liquid to the first quantitative container; Alternatively, The quantitative component further comprises a flow sensor, which is used to measure the flow rate of the liquid entering the first quantitative container from the first infusion pipeline; The processor calculates a target time according to the measured flow rate and the quantitative parameters of the reagent preparation this time, and when the target time is reached, the processor controls the quantitative component to stop supplying liquid to the first quantitative container.

13. The reagent preparation apparatus according to claim 11, wherein The quantitative component at least comprises a first quantitative container, a first infusion pipeline and a first liquid discharge pipeline; the first quantitative container is used to measure the first liquid and / or the second liquid; the first infusion pipeline is connected with the first quantitative container, and is used to deliver the liquid to be measured to the first quantitative container; the first liquid discharge pipeline is connected between the first quantitative container and the mixing component, and is used to discharge the liquid measured by the first quantitative container to the mixing component; The quantitative component further comprises a liquid level detection component; the liquid level detection component can detect the liquid surface height; The processor calculates a target height of the liquid surface according to the amount of the first liquid and / or the second liquid to be measured represented by the quantitative parameters of the reagent preparation this time, and when the liquid level detection component detects that the liquid enters the first quantitative container from the first infusion pipeline and the liquid surface reaches the target height, the processor controls the quantitative component to stop supplying liquid to the first quantitative container; Alternatively, The quantitative component further comprises a first liquid level detection component and a second liquid level detection component; the first liquid level detection component is used to detect whether the liquid level in the first quantitative container reaches a first liquid level, and the second liquid level detection component is used to detect whether the liquid level in the first quantitative container reaches a second liquid level, wherein the second liquid level is higher than the first liquid level; the processor can calculate the flow rate of the liquid entering the first quantitative container from the first infusion pipeline according to the volume change amount and the time when the liquid rises from the first liquid level to the second liquid level; the processor further calculates a target time according to the calculated flow rate and the quantitative parameters of the reagent preparation this time, and when the target time is reached, the processor controls the quantitative component to stop supplying liquid to the first quantitative container; Alternatively, The dosing component further comprises a flow sensor for measuring the flow rate of the liquid from the first infusion line into the first dosing container; The processor calculates a target time according to the measured flow rate and the dosing parameter of the current reagent preparation, and controls the dosing component to stop supplying the liquid to the first dosing container when the target time is reached.

14. The reagent preparation apparatus according to claim 12, wherein The first dosing container comprises a first body portion and a first nozzle portion, the first nozzle portion is arranged above the first body portion and communicates with the first body portion, and the inner diameter of the first body portion is greater than that of the first nozzle portion; If the dosing component comprises the liquid level detection component, the liquid level detection component is arranged in the first nozzle portion; If the dosing component comprises the first liquid level detection component and the second liquid level detection component, the first liquid level detection component and the second liquid level detection component are arranged in the first nozzle portion.

15. The reagent preparation apparatus according to claim 13, wherein The first dosing container comprises a first body portion and a first nozzle portion, the first nozzle portion is arranged above the first body portion and communicates with the first body portion, and the inner diameter of the first body portion is greater than that of the first nozzle portion; If the dosing component comprises the liquid level detection component, the liquid level detection component is arranged in the first nozzle portion; If the dosing component comprises the first liquid level detection component and the second liquid level detection component, the first liquid level detection component and the second liquid level detection component are arranged in the first nozzle portion.

16. A sample analysis system, comprising: Comprising: A plurality of sample analysis modules for determining samples; A track for connecting each sample analysis module; A scheduling device for scheduling samples transmitted through the track to corresponding sample analysis modules; The reagent preparation device according to any one of claims 1 to 15, for supplying reagents to the sample analysis modules.

17. A method of reagent preparation, characterized by, Comprising: Obtaining a dosing parameter of the Nth reagent preparation, the dosing parameter being used to represent the amount of the first liquid and the second liquid to be dosed; Wherein N is a positive integer; Dosing the first liquid and the second liquid according to the dosing parameter of the Nth reagent preparation to prepare a reagent with a target concentration; Detecting a concentration detection result of the Nth prepared reagent; Obtaining a temperature of the N+1th reagent preparation; the temperature comprises an ambient temperature, a first liquid temperature, and / or a second liquid temperature Calculating a dosing parameter of the N+1th reagent preparation according to the concentration detection result of the Nth prepared reagent and the temperature of the N+1th reagent preparation; Dosing the first liquid and the second liquid according to the dosing parameter of the N+1th reagent preparation to prepare a reagent with a target concentration.

18. The reagent preparation method according to claim 17, wherein The calculation of the dosing parameter of the N+1th reagent preparation comprises: The ratio of the amount of the first liquid and the amount of the second liquid; The amount of the first liquid, which comprises the amount of the first liquid in the preparation of the reagent, the change amount of the first liquid in the preparation of the reagent compared with the previous time, or the change amount of the first liquid in the preparation of the reagent compared with the default amount; The amount of the second liquid includes: the amount of the second liquid in the current preparation of the reagent, the changed amount of the second liquid in the current preparation of the reagent compared with the previous time, or the changed amount of the second liquid in the current preparation of the reagent compared with the default amount.

19. The reagent preparation method according to claim 17 or 18, wherein The first liquid is a stock solution of the reagent, and the second liquid is a diluent for diluting the stock solution of the reagent.

20. A method of reagent preparation, characterized by, The method comprises: obtaining a quantitative parameter of the Nth preparation of the reagent, the quantitative parameter being used to characterize the amounts of the first liquid and the second liquid that need to be measured; wherein N is a positive integer; measuring the first liquid and the second liquid according to the quantitative parameter of the Nth preparation of the reagent, so as to prepare the reagent with a target concentration; detecting a concentration detection result of the reagent prepared in the Nth preparation; calculating a quantitative parameter of the (N+1)th preparation of the reagent according to the concentration detection result of the reagent prepared in the Nth preparation; measuring the first liquid and the second liquid according to the quantitative parameter of the (N+1)th preparation of the reagent, so as to prepare the reagent with a target concentration.

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