Coated tannic acid preparation for weaned piglets and preparation method and use thereof

Abstract

The present invention belongs to the technical field of feed additives, and specifically relates to a coated tannic acid preparation for weaned piglets, and a preparation method and use thereof. In view of the existing problems that weaned piglets are prone to high diarrhea rate, low feed intake, reduced immune function, and poor growth, the present invention provides a coated tannic acid preparation for weaned piglets. The coated tannic acid preparation for weaned piglets described in the present invention comprises: by weight percentage, 70-80% of the main material and 20-30% of the auxiliary material; the main material is a mixture of tannic acid, chlorogenic acid and hesperidin; the auxiliary material is a mixture of corn starch, β-cyclodextrin, sodium alginate, palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate. The present invention also provides a preparation method and use of the above-mentioned preparation. The preparation of the present invention can improve the palatability of feed, reduce the diarrhea rate of weaned piglets, repair damaged intestines of weaned piglets, maintain the balance of intestinal microbial cells, and improve the disease resistance of weaned piglets.

Description

Technical Field

[0001] The invention belongs to the technical field of feed additives, and particularly relates to a coated tannic acid preparation for weaned piglets, a preparation method and application thereof. Background Art

[0002] In order to increase the number of litters per year, avoid cross-infection of diseases between sows and piglets, and increase economic benefits, early weaning is usually implemented for piglets in production. However, early weaning will cause stress to piglets, affect the morphological structure of piglet intestinal tissue and the balance of intestinal microorganisms, and easily cause problems such as decreased feed intake, digestive system disorders, and diarrhea. How to avoid diarrhea in weaned piglets and improve the intestinal health and disease resistance of weaned piglets has always been the focus of attention in the industry.

[0003] As my country has significantly reduced the amount of zinc oxide in feed and banned the use of growth-promoting drug feed additives, how to effectively prevent and control piglet diarrhea through other means has become one of the common difficulties faced by the entire industry. In the context of comprehensive "antibiotic restriction and ban", alternative antibiotic products have become an urgent need for the healthy development of weaned piglet farming.

[0004] Hydrolyzed tannins are also called tannic acid or tannic acid. The relative molecular weight is between 500 and 3000. They can be hydrolyzed into ellagic acid, gallic acid and glucose. As a polyphenolic compound, hydrolyzed tannins have strong biological properties and pharmacological activity. Domestic and foreign studies have shown that hydrolyzed tannins have great potential for application in animal nutrition. Hydrolyzed tannins from sweet chestnuts have been widely used as feed additives in Europe. The abuse of antibiotics in current animal breeding production has led to serious food safety problems and the generation of super bacteria. Hydrolyzed tannins, as pharmacologically active substances produced by plants, have antibacterial, antioxidant and free radical scavenging effects. They can be used as feed additives to replace antibiotics in animal feed. At present, studies have shown that adding 0.7-1.5kg / t of hydrolyzed tannins to the diet of weaned piglets can reduce diarrhea, improve production performance, reduce the amount of zinc oxide and replace antibiotics. However, due to the high content of tannic acid, it can combine with salivary mucin and precipitate, causing rough wrinkles and dryness, producing a strong bitter taste and poor palatability. It is easy to cause new problems such as decreased feed intake during the weaning period of piglets, thereby affecting the immune function of piglets and making them prone to other related diseases.

[0005] In view of the above problems, the present invention intends to produce a new additive specifically suitable for improving the disease resistance of weaned piglets by combining tannic acid, chlorogenic acid and hesperidin and adopting reasonable coating technology.

[0006] Chlorogenic acid, also known as coffee tannic acid, is widely found in dicotyledonous plants and ferns, especially in Eucommia ulmoides, honeysuckle, chrysanthemum and other plants. There are few plant sources, and due to the limitations of existing plant yields and content, the large supply of chlorogenic acid is limited, and the price is still high. In addition, the effect of using chlorogenic acid alone as a feed additive in production practice still cannot completely replace chemical antibiotics, and its disease resistance is poor.

[0007] Hesperidin, also known as hesperidin, is a flavonoid substance widely found in citrus fruits. Its chemical structure is a dihydroflavonoid oxygen glycoside structure, which is weakly acidic. The crude product obtained by extraction is a light yellow powder. The pure product is a white needle-shaped crystal, which is difficult to dissolve in water. Hesperidin has a broad-spectrum antibacterial effect on common food contaminating bacteria, and has a significant inhibitory effect on Bacillus subtilis, Salmonella typhimurium, Shigella flexneri, hemolytic Streptococcus and Vibrio cholerae, so it is widely used in food additives and food processing.

[0008] At present, there are no reports on the use of tannic acid, chlorogenic acid and hesperidin together as additives for weaned piglets, and development is urgently needed. Summary of the invention

[0009] The technical problem to be solved by the present invention is that the existing weaned piglets are prone to diarrhea, reduced feed intake and reduced immune function.

[0010] The technical solution of the present invention to solve the above technical problems is: to provide a coated tannic acid preparation for weaned piglets. The coated tannic acid preparation for weaned piglets of the present invention comprises: by weight percentage, 70-80% of main material and 20-30% of auxiliary material; the main material is a mixture of tannic acid, chlorogenic acid and hesperidin; the auxiliary material is a mixture of corn starch, β-cyclodextrin, sodium alginate, palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate.

[0011] Furthermore, in the above-mentioned coated tannic acid preparation for weaned piglets, the weight ratio of tannic acid, chlorogenic acid and hesperidin in the main ingredients is 6-10:2-4:0.5-3.

[0012] Preferably, in the above-mentioned coated tannic acid preparation for weaned piglets, the weight ratio of tannic acid, chlorogenic acid and hesperidin in the main ingredient is 9:3:2.

[0013] Wherein, in the coated tannic acid preparation for weaned piglets, the tannic acid is tannic acid with a purity of ≥50% extracted from gallnut, tara, chestnut or quebracho.

[0014] Wherein, in the above-mentioned coated tannic acid preparation for weaned piglets, the purity of chlorogenic acid is ≥95%.

[0015] Wherein, in the above-mentioned coated tannic acid preparation for weaned piglets, the purity of hesperidin is ≥20%.

[0016] Among them, in the coated tannic acid preparation for weaned piglets, the weight ratio of corn starch, β-cyclodextrin, sodium alginate, palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate in the auxiliary materials is 7-9:1-4:2-3:3-5:0.5-3:2-6. The preferred weight ratio is: 8:2:2:4:1:4.

[0017] Wherein, in the above-mentioned coated tannic acid preparation for weaned piglets, the acrylic resin is at least one of acrylic resin types I, II and III.

[0018] The present invention also provides a method for preparing the coated tannic acid preparation for weaned piglets, comprising the following steps:

[0019] a. Weigh β-cyclodextrin and sodium alginate, dissolve them in hot water, then add tannic acid, chlorogenic acid, hesperidin and corn starch, mix well, and spray granulate to obtain microspheres;

[0020] b. Heat and melt palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate to prepare a spraying liquid, add the microspheres into the fluidized bed, and spray the spraying liquid in a bottom boiling fluidized bed at a temperature of 45°C to 60°C for 15 to 20 minutes, and then coat and discharge.

[0021] Wherein, in the preparation method of the coated tannic acid preparation for weaned piglets, the granulation temperature in step a is 95-110° C. and the rotation speed is 600-700 rpm.

[0022] Wherein, in the preparation method of the coated tannic acid preparation for weaned piglets, the particle size of the microspheres described in step a is 90 to 100 meshes.

[0023] The present invention also provides a use of the coated tannic acid preparation for weaned piglets, which is used for preparing an additive or feed for improving the immunity of weaned piglets.

[0024] Wherein, in the above-mentioned use, the usage amount of the coated tannic acid preparation for weaned piglets is 200-500 mg / kg basic daily diet.

[0025] The beneficial effects of the present invention are:

[0026] The present invention provides a coated tannic acid preparation for weaned piglets, which is particularly aimed at the physiological characteristics of weaned piglets and adopts a combination of "tannic acid + chlorogenic acid + hesperidin". Hesperidin can neutralize the bitter taste of tannic acid and chlorogenic acid and improve the palatability of feed. The combination of the three can reduce the diarrhea rate of weaned piglets, repair the damaged intestines of weaned piglets, maintain the balance of intestinal microbial cells, and improve the disease resistance of weaned piglets. At the same time, the preparation of the present invention is also coated by a special method. By selecting suitable auxiliary materials and coating materials, the bitter taste of tannic acid and chlorogenic acid can be further concealed, and it can also be controlled and sustained-released, not degraded in the stomach, and directly degraded in the small intestine and colon. The coating material used in the present invention is non-toxic and harmless, can be digested, absorbed and utilized by piglets, and does not affect the absorption of other nutrients. DETAILED DESCRIPTION

[0027] The present invention designs a tannic acid additive specifically for weaned piglets to address the problems of easy diarrhea, poor feed intake, low immune function, etc. in weaned piglets. Conventional tannic acid preparations only use tannic acid or coated tannic acid, and the bitter taste of tannic acid will affect the palatability of feed and reduce feed intake. Tannic acid that reaches the intestines only relies on astringency to regulate diarrhea in piglets, and cannot comprehensively improve the immune ability of piglets.

[0028] In order to solve the above problems, the present invention reasonably combines "tannic acid, chlorogenic acid and hesperidin", fully utilizing the antibacterial and anti-inflammatory effects and good astringent effects of tannic acid, improving the intestinal health of piglets, and reducing the diarrhea rate of piglets. At the same time, chlorogenic acid can promote the expression of intestinal epithelial cells to play an antibacterial role, and also has a strong anti-inflammatory effect. At the same time, chlorogenic acid and hesperidin both have antioxidant effects and can maintain the diversity of the intestinal microbial flora of weaned piglets. Hesperidin also masks the bitter taste of tannic acid and chlorogenic acid, does not absorb moisture, is not easily oxidized, has good product stability, is easy to store and use, and has good fluidity.

[0029] The present invention also uses a special coating material to coat "tannic acid, chlorogenic acid and hesperidin", and the coating material is a mixture of corn starch, β-cyclodextrin, sodium alginate, palm oil, acrylic resin, and hydroxypropyl methylcellulose acetate succinate.

[0030] Corn starch can improve dispersibility, making tannic acid, chlorogenic acid and hesperidin more stable, better dispersed, and easier to mix when mixed with bulk raw materials.

[0031] β-cyclodextrin has a hydrophobic cavity and can form an inclusion complex. By forming a complex with tannic acid, chlorogenic acid and hesperidin, it can reduce oxidation, passivate photosensitivity and heat sensitivity, and reduce volatility. At the same time, β-cyclodextrin can also neutralize the bitter taste of tannic acid and improve the palatability of feed additives.

[0032] Sodium alginate is a high-polysaccharide uronic acid formed by the aldehyde group of β-D-mannuronic acid with a glycosidic bond. It is a natural macromolecular carbohydrate, non-toxic, with good disintegration effect, good water resistance and stability, and easy storage. It has good enteric effect and stable enteric performance, and is suitable for industrial production.

[0033] Acrylic resin has the advantages of safety, simple operation, good drying and good stability. It can also reduce the use of a large number of auxiliary materials. When it is mixed with hydroxypropyl methylcellulose acetate succinate, it can have good flexibility without adding plasticizers and is suitable for modeling.

[0034] Hydroxypropyl methylcellulose acetate succinate is used as both an enteric coating material and a polymer carrier. It has good film-forming properties and does not require the addition of plasticizers. It has good solubility in the upper part of the small intestine. It can effectively prevent the effective ingredients of "tannic acid, chlorogenic acid and hesperidin" from being degraded in the stomach and directly decompose in the posterior end of the intestine, which has a good sustained-release and controlled-release effect. At the same time, the presence of an appropriate amount of acetyl in the material will improve the flexibility of the free film of the HPMCAS coating material, improve the acid resistance, and prevent the material from agglomerating and sticking. At the same time, the presence of an appropriate amount of succinyl also improves the solubility of the free film of the HPMCAS coating material.

[0035] The specific implementation modes of the present invention will be further explained below through examples, but it is not intended to limit the protection scope of the present invention to the scope described in the examples.

[0036] All raw materials and equipment used in the examples are common commercially available products.

[0037] Example 1 Preparation of the coated tannic acid preparation for weaned piglets of the present invention

[0038] Weigh respectively: 4.5 kg of tannic acid, 1.5 kg of chlorogenic acid, 1 kg of hesperidin; 1.143 kg of corn starch, 0.286 kg of β-cyclodextrin, 0.286 kg of sodium alginate, 0.571 kg of palm oil, 0.143 kg of acrylic resin and 0.571 kg of hydroxypropyl methylcellulose acetate succinate.

[0039] β-cyclodextrin and sodium alginate were dissolved in hot water, and tannic acid, chlorogenic acid, hesperidin and corn starch were added, mixed evenly, and then spray granulated at a granulation temperature of 95°C and a rotation speed of 700 rpm to obtain microspheres with a particle size of 90 to 100 meshes;

[0040] Palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate are heated and melted to prepare a spraying liquid, and the microspheres are added into a fluidized bed. The spraying liquid is sprayed in a bottom boiling fluidized bed at a temperature of 45° C. for 20 minutes, and the coated material is discharged.

[0041] Example 2 Preparation of the coated tannic acid preparation for weaned piglets of the present invention

[0042] Weigh respectively: 480 g of tannic acid, 160 g of chlorogenic acid, 160 g of hesperidin; 70 g of corn starch, 10 g of β-cyclodextrin, 20 g of sodium alginate, 30 g of palm oil, 10 g of acrylic resin and 60 g of hydroxypropyl methylcellulose acetate succinate.

[0043] β-cyclodextrin and sodium alginate were dissolved in hot water, and tannic acid, chlorogenic acid, hesperidin and corn starch were added, mixed evenly, and then spray granulated at a granulation temperature of 110°C and a rotation speed of 600 rpm to obtain microspheres with a particle size of 90 to 100 meshes;

[0044] Palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate are heated and melted to prepare a spraying liquid, and the microspheres are added into a fluidized bed. The spraying liquid is sprayed in a bottom boiling fluidized bed at a temperature of 60° C. for 15 minutes, and the coated material is discharged.

[0045] Example 3 Preparation of the coated tannic acid preparation for weaned piglets of the present invention

[0046] Weigh respectively: 5 kg of tannic acid, 1 kg of chlorogenic acid, 1.5 kg of hesperidin; 0.9 kg of corn starch, 0.4 kg of β-cyclodextrin, 0.3 kg of sodium alginate, 0.5 kg of palm oil, 0.2 kg of acrylic resin and 0.2 kg of hydroxypropyl methylcellulose acetate succinate.

[0047] β-cyclodextrin and sodium alginate were dissolved in hot water, and tannic acid, chlorogenic acid, hesperidin and corn starch were added, mixed evenly, and then spray granulated at a granulation temperature of 100°C and a rotation speed of 650 rpm to obtain microspheres with a particle size of 90 to 100 meshes;

[0048] Palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate are heated and melted to prepare a spraying liquid, and the microspheres are added into a fluidized bed. The spraying liquid is sprayed in a bottom boiling fluidized bed at a temperature of 50° C. for 20 minutes, and the coated material is discharged.

[0049] Comparative Example 4 Preparation of a single coated tannic acid preparation

[0050] Comparative Example 4 is divided into 3 groups, group 4-1 is a group coated with tannic acid only, group 4-2 is a group coated with chlorogenic acid only, and group 4-3 is a group coated with hesperidin only. The tannic acid content of the tannic acid group is 4.5 kg, the chlorogenic acid content of the chlorogenic acid group is 1.5 kg, and the hesperidin content of the hesperidin group is 1 kg. The auxiliary material components, dosages, and preparation methods used are the same as those in Example 1.

[0051] Comparative Example 5 Preparation of Tannic Acid Preparations Using Different Coating Materials

[0052] Comparative Example 5 was divided into 7 groups. The main ingredients for coating were 4.5 kg of tannic acid, 1.5 kg of chlorogenic acid, and 1 kg of hesperidin. The coating method was the same as that of Example 1.

[0053] The coating material formula and dosage of each group are shown in Table 1 below.

[0054] Table 1 Preparation of tannic acid preparations using different coating materials (kg)

[0055]

[0056] Comparative Example 6: The coating method of the present invention is not used for coating

[0057] Weigh respectively: 4.5 kg of tannic acid, 1.5 kg of chlorogenic acid, 1 kg of hesperidin; 1.143 kg of corn starch, 0.286 kg of β-cyclodextrin, 0.286 kg of sodium alginate, 0.571 kg of palm oil, 0.143 kg of acrylic resin and 0.571 kg of hydroxypropyl methylcellulose acetate succinate.

[0058] β-cyclodextrin and sodium alginate were dissolved in hot water, and tannic acid, chlorogenic acid, hesperidin and corn starch were added, mixed evenly, and then spray granulated at a granulation temperature of 95°C and a rotation speed of 700 rpm to obtain microspheres with a particle size of 90 to 100 meshes;

[0059] Palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate are heated and melted, the melted liquid is mixed with the microspheres, and then freeze-sprayed to coat and discharge the microspheres.

[0060] Test Example 7 Stability Analysis of Each Coated Tannic Acid Preparation

[0061] The dissolution apparatus was used to simulate pig stomach and intestine digestion in vitro, and the cumulative release percentage of the in vitro release was used as the evaluation index to determine the digestive tract release of the tannic acid preparation.

[0062] The gastrointestinal dissolution of each embodiment and comparative example is shown in Table 2 below.

[0063] Table 2 Gastrointestinal cumulative release rate (%)

[0064] Stomach 1h Stomach 2h Stomach 4h Intestinal 1h Intestinal 2h Intestinal 4h Intestinal 8h Example 1 0.35 1.24 2.76 15.44 25.74 55.83 77.53 Example 2 0.33 1.57 2.53 13.12 24.93 53.11 75.13 Example 3 0.46 1.42 2.90 14.97 23.22 55.12 75.03 Comparative Example 4-1 0.38 1.33 2.87 15.11 26.12 54.17 72.10 Comparative Example 4-2 0.39 1.45 2.90 16.23 25.95 57.12 74.18 Comparative Example 4-3 0.41 1.49 2.56 15.60 26.74 57.29 69.66 Comparative Example 5-1 1.39 1.98 3.54 18.23 23.30 51.11 71.24 Comparative Example 5-2 0.35 1.76 2.69 15.74 22.78 49.48 72.02 Comparative Example 5-3 0.37 1.43 2.73 15.98 25.30 54.89 75.11 Comparative Example 5-4 3.45 12.09 22.23 34.89 42.81 64.02 72.10 Comparative Example 5-5 3.78 11.75 23.04 35.01 44.67 65.09 73.23 Comparative Examples 5-6 1.34 5.78 13.22 25.20 38.12 56.39 67.19 Comparative Examples 5-7 7.35 15.83 33.02 44.24 56.12 63.45 68.12 Comparative Example 6 2.98 4.23 10.55 34.13 41.23 54.24 63.92

[0065] It can be seen from the experimental results in Table 2 that: Examples 1-3 and Comparative Example 4 use the coating agent and coating method of the present invention, which can basically ensure that there is an extremely low dissolution rate in the stomach, and continuously disintegrate in the intestine, and the dissolution rate of 8h is above 70%, and the dissolution effect is good. In Comparative Example 5, due to the different components and proportions of the coating material, some groups will have a certain dissolution in the stomach, and the effective functional components reaching the intestine are reduced. In particular, the group without coating has a very high degradation rate in the stomach, reaching 33.02%; when any one of acrylic resin or hydroxypropyl methylcellulose acetate succinate is missing in the coating material, the dissolution rate in the stomach is also greatly improved. It can be seen that the coating material of the present invention needs to be used according to the formula amount under specific components to have a good effect of not degrading in the stomach and degrading in the intestine. The results of Comparative Example 6 also reflect the importance of the coating method. The coating method will also affect the gastrointestinal dissolution of the active ingredient. The method of the present invention can reduce the dissolution rate in the stomach compared with other coating methods.

[0066] Experimental Example 8 Effects of various coated tannic acid preparations on weaned piglets

[0067] 70 healthy three-way crossbred 30-day-old weaned piglets with an initial weight of (8.97±1.48) kg were selected and randomly divided into 14 groups, with 5 replicates in each group and 1 pig in each replicate. The coated tannic acid preparations prepared in Examples 1-3 and Comparative Examples 4-6 were added to the diets, respectively, and the feeding amount was 250 mg / kg of the coated tannic acid preparations added to the basic diet.

[0068] The trial period was 33 days, including a 5-day pre-trial period. The pigs were fed in a single stall and were allowed to eat and drink freely. The experimental diet was a common commercially available weaned pig basal diet.

[0069] The daily feeding amount and residual amount were accurately recorded. The piglets were weighed on an empty stomach at 8:00 am on the 1st to 28th day of the formal experiment. Blood was collected from the anterior vena cava in each group, anticoagulated with heparin sodium, and centrifuged at 4°C and 3000r / min for 20min to separate plasma. After blood collection, the pigs were slaughtered and the visceral tissues were removed to measure the length of the jejunum, ileum and colon.

[0070] Growth performance was calculated according to the following formula: average daily weight gain = total weight gain / days; average daily feed intake = total feed intake / days.

[0071] The effects of the tannic acid preparation of the present invention on the growth performance of weaned piglets are shown in Table 3 below.

[0072] Table 3 Effects of tannic acid preparations on growth performance of weaned piglets

[0073]

[0074]

[0075] From the results in Table 3, it can be seen that the composite tannic acid preparation of the present invention can more effectively increase the feed intake and daily weight gain of weaned piglets, and improve the growth performance of weaned piglets, compared with the single-ingredient preparation and the composite preparation of other coating ingredients in Comparative Example 4. In the experimental group fed with the composite tannic acid preparation, the diarrhea rate of piglets was also significantly reduced, and basically no piglets with diarrhea appeared, while in the group fed with the single-ingredient preparation, piglets still had diarrhea.

[0076] According to the kit instructions, the total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD), catalase (CAT), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in plasma were determined by colorimetry. The effects of the tannic acid preparation of the present invention on the biochemical indices of weaned piglet plasma are shown in Table 4 below.

[0077] Table 4 Effects of tannic acid preparations on plasma biochemical parameters of weaned piglets (U / ml)

[0078]

[0079]

[0080] From the results in Table 4, it can be seen that the composite preparation of the present invention can significantly enhance the antioxidant function of piglet plasma compared with the single use of tannic acid, chlorogenic acid or hesperidin. The possible reason for the present invention to enhance the antioxidant performance of piglets is that the addition of tannic acid, chlorogenic acid and hesperidin composite preparation can increase the feed intake of piglets, and the intake of sufficient protein and energy promotes the growth and development of the body; in addition, chlorogenic acid and hesperidin themselves have antioxidant functions. The combination of the two, combined with tannic acid, can improve the body's antioxidant capacity, reduce the level of cell membrane lipid reaction, protect the integrity of the cell membrane, maintain normal liver function, and reduce the escape of ALT and AST from tissue cells.

[0081] The effects of the tannic acid preparation of the present invention on the intestinal mucosal tissue morphology of weaned piglets are shown in Table 5 below.

[0082] Table 5 Effects of tannic acid preparations on the intestinal mucosal tissue morphology of weaned piglets (um)

[0083] Jejunal villus height Ileal villus height Colon villus height Example 1 305 296 231 Example 2 311 287 227 Example 3 308 298 229 Comparative Example 4-1 265 244 198 Comparative Example 4-2 254 253 195 Comparative Example 4-3 233 245 201 Comparative Example 5-1 287 265 211 Comparative Example 5-2 285 271 205 Comparative Example 5-3 292 267 206 Comparative Example 5-4 279 262 212 Comparative Example 5-5 276 271 215 Comparative Examples 5-6 278 267 216 Comparative Examples 5-7 241 255 199 Comparative Example 6 287 251 215

[0084] From the results in Table 5, it can be seen that feeding the composite tannic acid preparation of the present invention can increase the height of the villi in the jejunum, ileum and colon of piglets, promote the development of intestinal villi, and reduce villi damage. The results show that the composite tannic acid preparation of the present invention has the effect of alleviating the intestinal stress response of piglets after weaning. The mechanism of action may be related to the antioxidant function, which reduces the level of oxidative stress in the body to avoid oxidative damage to the intestine, promotes intestinal development, and improves the integrity of the intestinal morphological structure.

[0085] In summary, the coated tannic acid preparation for weaned piglets designed by the present invention can avoid degradation in the stomach, and the effective ingredients have a good dissolution rate in the intestine, which can promote weaned piglets to increase feed intake and daily weight gain, enhance growth performance, and also improve antibacterial ability and antioxidant level, reduce oxidative damage to the intestine, improve the integrity of the intestinal morphology and structure, and keep the body healthier, which has great practical significance.

Claims

1. A coated tannic acid preparation for weaned piglets, characterized in that: Composition includes: In terms of weight percentage, the main material is 70-80%, and the auxiliary material is 20-30%; the main material is a mixture of tannic acid, chlorogenic acid and hesperidin, and the weight ratio of tannic acid, chlorogenic acid and hesperidin in the main material is 6-10:2-4:0.5-3; the auxiliary material is a mixture of corn starch, β-cyclodextrin, sodium alginate, palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate, and the weight ratio of corn starch, β-cyclodextrin, sodium alginate, palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate in the auxiliary material is 7-9:1-4:2-3:3-5:0.5-3:2-6; the preparation method of the coated tannic acid preparation for weaned piglets comprises the following steps: a. Weigh β-cyclodextrin and sodium alginate, dissolve them in hot water, add tannic acid, chlorogenic acid, hesperidin and corn starch, mix well, and spray granulate to obtain microspheres; b. Heat and melt palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate to prepare a spraying liquid, add the microspheres into the fluidized bed, and spray the spraying liquid in a bottom boiling fluidized bed at a temperature of 45°C to 60°C for 15 to 20 minutes, and then coat and discharge.

2. The coated tannic acid preparation for weaned piglets according to claim 1, characterized in that: The weight ratio of tannic acid, chlorogenic acid and hesperidin in the main ingredient is 9:3:

2.

3. The coated tannic acid preparation for weaned piglets according to claim 1, characterized in that: The tannic acid is tannic acid extracted from gallnut, tara, chestnut or quebracho with a purity of ≥50%; the purity of chlorogenic acid is ≥95%; and the purity of hesperidin is ≥20%.

4. The coated tannic acid preparation for weaned piglets according to claim 1, characterized in that: The weight ratio of corn starch, beta-cyclodextrin, sodium alginate, palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate in the auxiliary materials is 8:2:2:4:1:

4.

5. The coated tannic acid preparation for weaned piglets according to claim 1, characterized in that: The granulation temperature in step a is 95-110° C. and the rotation speed is 600-700 rpm.

6. The coated tannic acid preparation for weaned piglets according to claim 1, characterized in that: The particle size of the microspheres described in step a is 90 to 100 meshes.

7. Use of the coated tannic acid preparation for weaned piglets according to any one of claims 1 to 6 in the preparation of an additive or feed for improving immunity of weaned piglets.

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