Anthocyanin-containing gel mask and use method thereof

Abstract

The present invention relates to the field of facial mask technology, and specifically to a gel mask containing anthocyanidins and a method for using the same. The mask comprises a component A and a component B, wherein the component A comprises 10-50 parts of freeze-dried blueberry powder; and the component B comprises an acid solution comprising the following components: 0.05-0.5 parts of α-hydroxy acid, 2-8 parts of glycerol, 20-50 parts of a blueberry wine extract, 10-50 parts of sodium carboxymethyl cellulose, 0.06-0.08 parts of potassium sorbate, and 50-90 parts of water. The gel mask containing anthocyanidins of the present invention utilizes separate packaging of the freeze-dried blueberry powder and the acid solution, ensuring that the anthocyanidins retain their antioxidant activity before use. The mask is packaged in ampoules and stored in a dark, refrigerated environment, extending its shelf life. The mask also utilizes ethanol from the blueberry wine extract as a solution for dissolving the anthocyanidins, avoiding the use of industrial alcohol and preventing excessive methanol content from the raw materials.

Description

Technical Field

[0001] The invention relates to the technical field of facial masks, and in particular to a gel facial mask containing anthocyanidins and a use method thereof. Background Art

[0002] Anthocyanidins, also known as anthocyanidins, are water-soluble natural pigments that are widely found in plants when they are combined with sugars through glycosidic bonds. They are a type of bioflavonoid with a unique molecular structure. They exist primarily as anthocyanins in plants and are internationally recognized as the most effective natural antioxidant for scavenging free radicals in the human body. They have very strong biological activity and are well absorbed, with 100% bioavailability in the human body. The antioxidant benefits of anthocyanidins are due to the hydrolysis of anthocyanins into free anthocyanidins, which carry oxonium ions that have antioxidant activity. Oxonium ions are highly active and easily oxidized and decomposed into other substances.

[0003] With the continuous improvement of people's living standards, modern urbanites are paying more and more attention to skin care and beauty. This awakening and pursuit of beauty has led to a surge in the development of my country's cosmetics industry. Facial masks, as a representative skin care product, have evolved from a special care product to a fast-moving consumer product, occupying a significant share of the beauty market.

[0004] Existing anthocyanin facial masks use anthocyanin extracts or synthetic proanthocyanidins. Free anthocyanidins are low in content and weak in antioxidant properties. They are also unstable and easily hydrolyzed or decomposed by light. Therefore, typical anthocyanin facial masks are directly made into aqueous products, which have a short shelf life and easily lose their antioxidant effects. Synthetic anthocyanidins exist in the form of artificially synthesized high-molecular polymers. While they have some antioxidant effects when taken as food, their effectiveness as cosmetics is significantly different from that of free anthocyanidins. Summary of the Invention

[0005] In view of this, the object of the present invention is to provide a gel mask containing anthocyanidins, which is packaged separately from blueberry freeze-dried powder and acid solution, ensuring that the anthocyanidins still have antioxidant activity before use, thereby improving the antioxidant and whitening effects of the gel mask; using ampoule packaging and light-proof refrigerated storage to extend the shelf life of the gel mask, effectively solving the problem of effective use of anthocyanidins as active substances in the field of facial masks; using ethanol in blueberry wine extract as a solution for dissolving anthocyanidins, blueberry wine extract is an organic natural raw material, avoiding the use of industrial alcohol, and preventing the problem of methanol exceeding the standard from the raw material.

[0006] In order to achieve the above technical objectives, the above objectives of the present invention are achieved through the following technical solutions:

[0007] A gel mask containing anthocyanidins comprises a component A and a component B, wherein the component A is 10-50 parts of freeze-dried blueberry powder; the component B is an acid solution comprising the following components: 0.05-0.5 parts of α-hydroxy acid, 2-8 parts of glycerol, 20-50 parts of blueberry wine extract, 10-50 parts of sodium carboxymethyl cellulose, 0.06-0.08 parts of potassium sorbate, and 50-90 parts of water.

[0008] Preferably, the α-hydroxy acid is one or more of citric acid, mandelic acid, glycolic acid, malic acid, and tartaric acid.

[0009] Preferably, the α-hydroxy acid is a mixture of mandelic acid and glycolic acid in a weight ratio of 0.1-0.2:0.05-0.1.

[0010] Preferably, it comprises component A and component B, wherein component A is 15-50 parts of blueberry freeze-dried powder; and component B is an acid solution comprising the following components: 0.05-0.5 parts of α-hydroxy acid, 2-8 parts of glycerol, 20-50 parts of blueberry wine extract, 10-50 parts of sodium carboxymethyl cellulose, 0.06-0.08 parts of potassium sorbate and 50-90 parts of water.

[0011] Preferably, it comprises component A and component B, wherein component A is 15-50 parts of blueberry freeze-dried powder; and component B is an acid solution comprising the following components: 0.1-0.2 parts of mandelic acid, 0.05-0.1 parts of glycolic acid, 2-8 parts of glycerol, 20-50 parts of blueberry wine extract, 10-50 parts of sodium carboxymethyl cellulose, 0.06-0.08 parts of potassium sorbate and 50-90 parts of water.

[0012] Freeze-dried blueberry powder contains delphinidin, petunia pigment, cyanidin, peony pigment, malvidin and malvidin. After acid extraction, free anthocyanidin is generated; mandelic acid provides acidic H + Ions make anthocyanins free, which can lighten melanin, remove acne marks and spots, and has antibacterial effects, which can prevent the precipitation of Gram-negative bacteria; glycolic acid provides acidic H + Ions free anthocyanins, which promote epidermal cell metabolism, improve pigmentation and whiten the skin; glycerol has a moisturizing effect; blueberry wine extract as a solution contains blueberry anthocyanins and fruit wine yeast products, which have the effect of whitening and lightening spots; sodium carboxymethyl cellulose as an emulsifier can suspend and mix the ingredients in the system evenly; potassium sorbate has an antibacterial effect and works synergistically with mandelic acid.

[0013] Preferably, components A and B are packaged in ampoules, light-proofed, and stored refrigerated away from light. Components A and B are packaged separately and packaged in ampoules, light-proofed, and stored refrigerated away from light. Separate packaging can prevent anthocyanidins from decomposing in water, thereby extending the shelf life of components A and B. Furthermore, refrigerated storage away from light can prevent rapid decomposition of anthocyanidins in sunlight or high temperatures.

[0014] Another object of the present invention is to provide a method for using a gel mask containing anthocyanidins, which is simple and convenient to operate and can achieve good free radical removal, freckle removal and whitening effects.

[0015] In order to achieve the above technical objectives, the above objectives of the present invention are achieved through the following technical solutions:

[0016] The invention discloses a method for using a gel mask containing anthocyanidins. When using the gel mask, component B is added to component A, and the mixture is shaken to obtain a gel-like mask liquid, which is then applied to the face.

[0017] Preferably, the shaking time is 3-5 minutes. If the A and B components are shaken and mixed for less than 3 minutes, the acidification effect of the anthocyanidin is poor, and if the shaking and mixing is continued for more than 5 minutes, the acidification effect of the anthocyanidin will not be significantly improved. Therefore, the optimal shaking time is controlled within 3-5 minutes.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] 1. The anthocyanidin-containing gel mask of the present invention utilizes blueberry freeze-dried powder and an acid solution to be packaged separately, thereby ensuring that the anthocyanidin still has antioxidant activity before use, thereby improving the antioxidant and whitening effects of the gel mask;

[0020] 2. The anthocyanidin-containing gel mask of the present invention is packaged in ampoules and stored in a dark and refrigerated environment, which reduces the oxidation of anthocyanidins by oxygen in the air or ultraviolet rays in the sun, prolongs the shelf life of the gel mask, and effectively solves the problem of effective use of anthocyanidins as active substances in the field of facial masks.

[0021] 3. The anthocyanidin-containing gel mask of the present invention uses ethanol from blueberry wine extract as a solution for dissolving anthocyanidins. Blueberry wine extract is an organic natural raw material, avoiding the use of industrial alcohol and preventing excessive methanol content in the raw material.

[0022] 4. The method of using the anthocyanin-containing gel mask of the present invention is simple, convenient and quick, and can achieve good free radical removal, freckle removal and whitening effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is the opaque packaging bag for the gel mask;

[0024] Figure 2 These are brown ampoules of component A and component B;

[0025] Figure 3 This is a full scan of alcohols using gas chromatography-mass spectrometry. DETAILED DESCRIPTION

[0026] In order to facilitate the understanding of those skilled in the art, the following embodiments and accompanying drawings are provided. Figure 1-3 The present invention is further described, and the contents mentioned in the embodiments are not intended to limit the present invention.

[0027] Example 1

[0028] A gel mask containing anthocyanidins comprises a component A and a component B, wherein the component A is 10 parts of freeze-dried blueberry powder; the component B is an acid solution comprising the following components: 0.05 parts of α-hydroxy acid, 2 parts of glycerol, 20 parts of blueberry wine extract, 10 parts of sodium carboxymethyl cellulose, 0.06 parts of potassium sorbate and 50 parts of water.

[0029] The α-hydroxy acid is a mixture of citric acid, malic acid and tartaric acid in a weight ratio of 0.01:0.02:0.02.

[0030] The components A and B are packaged in ampoules, light-proofed, and stored in a dark and refrigerated place. When the gel mask is used, the component B is added to the component A and shaken for 3 minutes to obtain the anthocyanidin-containing gel mask.

[0031] Example 2

[0032] A gel mask containing anthocyanidins comprises a component A and a component B, wherein the component A is 50 parts of freeze-dried blueberry powder; the component B is an acid solution comprising the following components: 0.5 parts of α-hydroxy acid, 8 parts of glycerol, 50 parts of blueberry wine extract, 50 parts of sodium carboxymethyl cellulose, 0.08 parts of potassium sorbate and 90 parts of water.

[0033] The α-hydroxy acid is a mixture of citric acid, mandelic acid and glycolic acid in a weight ratio of 0.2:0.2:0.1.

[0034] The components A and B are packaged in ampoules, light-proofed, and stored in a dark and refrigerated place. When the gel mask is used, the component B is added to the component A and shaken for 5 minutes to obtain the anthocyanidin-containing gel mask.

[0035] Example 3

[0036] A gel mask containing anthocyanidins comprises a component A and a component B, wherein the component A is 10 parts of freeze-dried blueberry powder; the component B is an acid solution comprising the following components: 0.25 parts of α-hydroxy acid, 2 parts of glycerol, 20 parts of blueberry wine extract, 10 parts of sodium carboxymethyl cellulose, 0.06 parts of potassium sorbate and 50 parts of water.

[0037] The α-hydroxy acid is a mixture of mandelic acid, glycolic acid and malic acid in a weight ratio of 0.1:0.05:0.1.

[0038] The components A and B are packaged in ampoules, light-proofed, and stored in a dark and refrigerated place. When the gel mask is used, the component B is added to the component A and shaken for 3 minutes to obtain the anthocyanidin-containing gel mask.

[0039] Example 4

[0040] A gel mask containing anthocyanidins comprises a component A and a component B, wherein the component A is 20 parts of blueberry freeze-dried powder; the component B is an acid solution comprising the following components: 0.24 parts of α-hydroxy acid, 5 parts of glycerol, 30 parts of blueberry wine extract, 30 parts of sodium carboxymethyl cellulose, 0.04 parts of potassium sorbate, and 70 parts of water.

[0041] The α-hydroxy acid is a mixture of mandelic acid and glycolic acid in a weight ratio of 0.16:0.08.

[0042] The components A and B are packaged in ampoules, light-proofed, and stored in a dark and refrigerated place. When the gel mask is used, the component B is added to the component A and shaken for 3 minutes to obtain the anthocyanidin-containing gel mask.

[0043] The contents of six anthocyanidins (delphinidin (Del), cyanidin (Cy), malvidin (Pet), petuniadin (Pel), malvidin (Mal) and peonydin (Peo)) in the gel mask were determined:

[0044] Liquid chromatography conditions: chromatographic column: C18 (150 mm × 2.1 mm L, 1.8 μm), mobile phase: phase A - 0.5% formic acid water, phase B - acetonitrile, flow rate: 0.3 mL / min, column temperature 40 ° C, injection volume: 2 μL, elution method:

[0045] Gradient elution was performed with an initial concentration of phase B of 0%. The time program is shown in Table 1.

[0046] Table 1 Gradient elution time program

[0047] Time(min) Module Command Value 1.0 Pumps Pump B Conc. 5 10.0 Pumps Pump B Conc. 20 16.0 Pumps Pump B Conc. 25 18.0 Pumps Pump B Conc. 40 19.0 Pumps Pump B Conc. 100 20.0 Pumps Pump B Conc. 100 21.0 Pumps Pump B Conc. 0 27.0 Controller Stop

[0048] LCMS-8045 mass spectrometry conditions: ion source: ESI (+), interface voltage: 4 kV, nebulizer gas flow rate: 3.0 L / min, heating block temperature: 400 °C, heating gas flow rate: 10.0 L / min, scan mode: multiple reaction monitoring (MRM), interface temperature: 300 °C, drying gas flow rate: 10.0 L / min, DL temperature: 250 °C, MRM parameters: see Table 2.

[0049] Table 2 Mass spectrometry parameters of six anthocyanidins

[0050]

[0051]

[0052] (1) Preparation of standard solution

[0053] Weigh appropriate amounts of anthocyanidin mixed standards, dissolve them in methanol, prepare a 1.0 mg / mL mixed standard stock solution, and store it in a -20°C refrigerator.

[0054] The standard stock solution was diluted with methanol to obtain a 10 μg / mL mixed standard solution. A series of standard solutions with concentrations of 0.1, 0.2, 0.5, 1.0, and 2.0 μg / mL were prepared by stepwise dilution.

[0055] (2) Sample pretreatment method

[0056] Weigh 10 g of the facial mask sample prepared in Example 4 (accurate to 0.01 g) into a 50.0 mL brown centrifuge tube, add 20 mL of 80% methanol solution, mix, ultrasonicate at room temperature for 15 min, centrifuge at 9000 r / min for 5 min, transfer the supernatant to a 50.0 mL brown volumetric flask, and extract the residue with 15 mL of 80% methanol acidic solution according to the above steps once. Combine the two extracts, make the volume to 50.0 mL with 80% methanol solution for later use, pass through a 0.22 μm membrane before loading on the machine, and dilute the sample so that the concentration on the machine is within the linear range of the standard curve.

[0057] The packaging of the gel mask in Example 4 is a light-proof packaging bag (such as Figure 1 As shown), both component A and component B are packaged in brown ampoules (as shown Figure 2 shown).

[0058] The following tests were performed on Example 4 in accordance with the three testing standards of "Technical Specification for Safety of Cosmetics (2015 Edition)", QBT2872-2017 and TSHRH006-2018:

[0059] 1. According to the requirements of Clause 5 of QB / T 2872-2017, perform the following test verification: dilute according to the direct method of Clause 6.1.5 of GB / T13531.1, place the sample in a beaker and set aside.

[0060] 1) Packaging materials and carriers

[0061] The brown ampoule used in Example 4 is a glass bottle package, without any other material carrier, which complies with the requirement of QB / T2872-2017 that the carrier does not contain migratable fluorescent whitening agents.

[0062] 2) Raw materials

[0063] The raw materials used in Example 4 are food-grade additives, and no flavoring is added, which meets the requirements of QB / T2872-2017.

[0064] 3) Sensory, physical, chemical and hygiene indicators

[0065] 3.1) Appearance

[0066] In the sensory laboratory, sensory indexes were tested using a comprehensive evaluation method conducted by three inspectors. The results showed that the products met the requirements. The appearance test results are shown in Table 3, and the aroma test results are shown in Table 4.

[0067] Table 3 Appearance inspection results

[0068]

[0069] Table 4 Aroma detection results

[0070]

[0071] 3.2) Physical and chemical indicators

[0072] 3.2.1) pH (25°C)

[0073] Measurements were performed using a Mettler-Toledo (Shanghai) PE-20K pH meter and an InLab Expert Pro pH meter probe. The pH values ​​were within the valid testing period. Mettler-Toledo pH 4.00, pH 7.00, and pH 9.00 buffer solutions were packaged. The average result of three measurements was 4.8, which meets the QB / T 2872-2017 requirement of 4.0 to 8.5 for gel masks.

[0074] 3.2.2) Heat resistance

[0075] A BGZ-246 electric heating blast thermostat from Shanghai Boxun Company was used, within a calibration instrument with a temperature control accuracy of ±1°C. Two portions of Example 4 were unpacked and placed in a 40°C thermostat without mixing. After 24 hours, one portion was removed and allowed to return to room temperature. Visual comparison with the other portion at room temperature revealed no significant differences, meeting the requirements of QB / T 2872-2017.

[0076] 3.2.3) Cold resistance

[0077] A Zhongke Duling Company MDF-25V328E medical cryogenic storage box was used, within a calibrated temperature chamber with a temperature control accuracy of ±1°C. Two portions of Example 4 were unpacked and placed in a -8°C storage box without mixing. After 24 hours, one portion was removed and allowed to return to room temperature. Visual comparison with the other portion at room temperature revealed no significant differences, meeting the requirements of QB / T 2872-2017.

[0078] 3.3) Hygiene indicators

[0079] 3.3.1) Alcohols

[0080] According to the determination method of GB / T 7917.4-1987 "Standard Test Methods for Hygienic Chemical Content of Cosmetics - Methanol", a Thermo Scientific Trace1310 gas chromatograph coupled with an ISQ single quadrupole mass spectrometer and a DB-FFAP capillary column (30 m × 0.25 mm, 0.25 μm) was used.

[0081] First, mix the gel mask of Example 4. After mixing, take 1g and dilute it to 50mL with chromatographically pure ethanol (Shanghai Aladdin Company). Take 1mL and pass it through a 0.22um organic filter membrane. Then, test it on the machine. The methanol content is <1mg / kg, which meets the requirement of <2000mg / kg in the "Technical Specifications for Safety of Cosmetics". The test results are as follows: Figure 3 .

[0082] Depend on Figure 3 Analysis showed that methanol was a spiked substance and was not detected in the gel mask of Example 4. The remaining eight compounds (propanol, isobutanol, 2-methylbutanol, isopentanol, 2,3-butanediol, propylene glycol, furfuryl alcohol and β-phenylethanol) were alcohols contained in the blueberry wine extract (except ethanol).

[0083] 3.3.2) Total colony count

[0084] According to Chapter 5 "Microbiological Testing Methods" of the "Technical Specifications for Safety of Cosmetics", Clause 6 "Test Methods for Mold and Yeast", it meets the requirement of ≦100 CFU / g. The test results are shown in Table 5.

[0085] Table 5 Total colony count test results

[0086]

[0087]

[0088] 3.3.3) Total number of molds and yeasts

[0089] According to Chapter 5 "Microbiological Testing Methods" of the "Technical Specifications for Safety of Cosmetics", Clause 6 "Test Methods for Mold and Yeast", it meets the requirement of ≦100 CFU / g. The test results are shown in Table 6.

[0090] Table 6 Detection results of total mold and yeast count

[0091]

[0092] 3.3.4) Thermotolerant coliform bacteria

[0093] According to Chapter 5 "Microbiological Testing Methods" of the "Technical Specifications for Safety of Cosmetics", Clause 3 "Thermotolerant Coliform Group Testing Method", it meets the requirement of not being detected. The test results are shown in Table 7.

[0094] Table 7 Heat-resistant coliform bacteria detection results

[0095]

[0096] 3.3.5) Staphylococcus aureus

[0097] According to Chapter 5 "Microbiological Testing Methods" of the "Technical Specifications for Safety of Cosmetics", Article 5 "Test Methods for Staphylococcus aureus", it meets the requirement of not being detected. The test results are shown in Table 8.

[0098] Table 8 Staphylococcus aureus detection results

[0099]

[0100]

[0101] 3.3.6) Pseudomonas aeruginosa

[0102] According to Chapter 5 "Microbiological Testing Methods" of the "Technical Specifications for Safety of Cosmetics", Article 4 "Test Methods for Pseudomonas aeruginosa", it meets the requirement of not being detected. The test results are shown in Table 9.

[0103] Table 9 Pseudomonas aeruginosa detection results

[0104]

[0105] 3.3.7) Lead, cadmium, mercury and arsenic

[0106] Instruments used: AA-6800F atomic absorption spectrophotometer (flame / graphite furnace) produced by Shimadzu Corporation of Japan; MDS-8 multi-flux closed microwave chemical workstation produced by Shanghai Xinyi Microwave Chemical Technology Co., Ltd.; lcs-35plus electric hot plate produced by Lichen Technology; GD20 graphite acid remover produced by Aople Company.

[0107] Mercury was tested using the "Mercury First Method Hydride Atomic Fluorescence Spectrometry" method specified in Section 1.2 of the "Safety Technical Specifications for Cosmetics." Using microwave digestion, two 0.5000g samples were weighed and placed in a Teflon cup. 1mL of water, 2mL of nitric acid, and 2mL of hydrogen peroxide were added. The sample was heated in a boiling water bath at 100°C for 20 minutes and then placed in a microwave digester for 5 minutes to complete digestion. The hot plate was used to remove interferences, and the sample was rinsed, then fixed to volume and loaded onto the digester. The results for the two replicate samples were N / A (Not Detected), reported as "Not Detected (<0.002μg / g)" according to the standard. This meets the "Safety Technical Specifications for Cosmetics" requirement of <1mg / kg.

[0108] Lead was tested using the "Lead First Method Graphite Furnace Atomic Absorption Spectrophotometry" method specified in Section 1.3 of the "Safety Technical Specifications for Cosmetics." Using microwave digestion, two 0.3000g samples were weighed and placed in a Teflon cup. 1mL of water, 2mL of nitric acid, and 2mL of hydrogen peroxide were added. The sample was heated in a boiling water bath at 100°C for 20 minutes and then placed in a microwave digester for 5 minutes to complete digestion. The hot plate was used to remove interferences, and the sample was rinsed, then fixed to volume and loaded onto the digester. The results for the two replicate samples were N / A (Not Detected), reported as "Not Detected (<0.05mg / kg)" according to the standard. This meets the "Safety Technical Specifications for Cosmetics" requirement of <10mg / kg.

[0109] Arsenic was detected using the "Arsenic First Method Hydride Atomic Fluorescence Spectrometry" method specified in Section 1.4 of the "Safety Technical Specifications for Cosmetics." Using microwave digestion, two 0.5000g samples were weighed and placed in a Teflon cup. 1mL of water, 2mL of nitric acid, and 2mL of hydrogen peroxide were added. The samples were heated in a boiling water bath at 100°C for 20 minutes and then placed in a microwave digester for 5 minutes to complete digestion. The hot plate was used to remove interferences, and the samples were rinsed, then fixed to volume and loaded onto the digester. The results for both replicates were N / A (Not Detected), reported as "Not Detected (<0.001μg / g)" according to the standard. This meets the "Safety Technical Specifications for Cosmetics" requirement of <2mg / kg.

[0110] Cadmium was tested using the "Cadmium" test in Section 1.5 of the "Safety Technical Specifications for Cosmetics." Using microwave digestion, two 0.5000g samples were weighed and placed in a Teflon cup. 1mL of water, 2mL of nitric acid, and 2mL of hydrogen peroxide were added. The sample was heated in a boiling water bath at 100°C for 20 minutes and then placed in a microwave digester for 5 minutes to complete digestion. The hot plate was used to remove interference, and the sample was rinsed, then fixed to volume and loaded onto the digester. The results for both replicates were N / A (Not Detected), reported as "Not Detected (<0.018mg / kg)" according to the standard. This meets the "Safety Technical Specifications for Cosmetics" requirement of <5mg / kg.

[0111] 2. Antioxidant test of the gel mask of Example 4 was performed according to T / SHRH006-2018:

[0112] Instruments used: analytical balance: sensitivity 0.1 mg; test tube: 10 mL; adjustable pipette: 1.00 mL; UV-visible spectrophotometer.

[0113] Reagents used: 95% ethanol; 1,1-diphenyl-2-trinitrophenylhydrazine (Sigma); 0.12 mg / mL DPPH ethanol solution: Weigh 12 mg of 1,1-diphenyl-2-trinitrophenylhydrazine into a 250 mL beaker, add 100 mL of 95% ethanol, and stir with a glass rod until dissolved. Positive control: Vitamin E, purity ≥96%.

[0114] Vitamin E was dissolved and diluted with 95% ethanol to form a series of concentration gradients of 0.08 mg / mL, 0.04 mg / mL, 0.02 mg / mL and 0.01 mg / mL for verification.

[0115] Gel Mask Treatment: Dilute the gel mask solution with water to create multiple concentrations. Use 10mL test tubes to set up the sample tube (T), sample background (T0), DPPH tube (C), and solvent background (C0). For each sample, set up three parallel tubes for each tested concentration of the sample tube (T), and three parallel tubes for the DPPH tube (C). Add 1mL of the same concentration of sample solution to each tube (T) and sample background (T0). Add water to all test tubes (T, T0, C, and C0) to a total of 3mL and mix thoroughly. Add 1mL of DPPH ethanol solution to the sample tube (T) and DPPH tube (C). Replace the sample background (T0) and solvent background (C0) with 95% ethanol. Gently shake and let stand at room temperature for 5 minutes. Transfer each reaction solution into a 1cm cuvette and measure the absorbance at 517nm. The results are shown in Table 10.

[0116] Table 10 Absorbance values

[0117]

[0118] Calculate the DPPH free radical scavenging rate:

[0119]

[0120] In the formula: T-sample tube absorbance, that is, the absorbance of the solution after the sample reacts with DPPH; T0-sample background absorbance; the average of three DPPH tube absorbance values, that is, the absorbance of the DPPH solution when no sample is added; C0-solvent background absorbance.

[0121] According to calculations, the clearance rate of sample 1 was 51.44%, the clearance rate of sample 2 was 47.34%, and the average clearance rate was 49.39%.

[0122] The technical solutions provided by the embodiments of the present invention are introduced in detail above. Specific examples are used herein to illustrate the principles and implementation methods of the embodiments of the present invention. The description of the above embodiments is only applicable to help understand the principles of the embodiments of the present invention. At the same time, for those skilled in the art, according to the embodiments of the present invention, there may be changes in the specific implementation methods and application scopes. In summary, the contents of this specification should not be understood as limiting the present invention.

Claims

1. A gel mask containing anthocyanidins, characterized in that: The method comprises component A and component B, wherein component A is 10-50 parts of freeze-dried blueberry powder; The component B is an acid solution, which includes the following components: 0.05-0.5 parts of α-hydroxy acid, 2-8 parts of glycerol, 20-50 parts of blueberry wine extract, 10-50 parts of sodium carboxymethyl cellulose, 0.06-0.08 parts of potassium sorbate and 50-90 parts of water; the α-hydroxy acid is a mixture of mandelic acid and glycolic acid in a weight ratio of 0.1-0.2:0.05-0.1; when using the gel mask, add component B to component A, shake well to obtain a gel-like mask liquid, and apply it to the face.

2. The anthocyanidin-containing gel mask according to claim 1, wherein: The method comprises component A and component B, wherein component A is 15-50 parts of freeze-dried blueberry powder; The component B is an acid solution, which includes the following components: 0.05-0.5 parts of α-hydroxy acid, 2-8 parts of glycerol, 20-50 parts of blueberry wine extract, 10-50 parts of sodium carboxymethyl cellulose, 0.06-0.08 parts of potassium sorbate and 50-90 parts of water.

3. The anthocyanidin-containing gel mask according to claim 1, wherein: The invention comprises a component A and a component B, wherein the component A is 15-50 parts of blueberry freeze-dried powder; the component B is an acid solution, and the acid solution comprises the following components: 0.1-0.2 parts of mandelic acid, 0.05-0.1 parts of glycolic acid, 2-8 parts of glycerol, 20-50 parts of blueberry wine extract, 10-50 parts of sodium carboxymethyl cellulose, 0.06-0.08 parts of potassium sorbate and 50-90 parts of water.

4. The anthocyanidin-containing gel mask according to claim 1, wherein: The components A and B are packaged in ampoules, light-proofed, and stored in a refrigerated place away from light.

5. The anthocyanidin-containing gel mask according to claim 1, wherein: The shaking time is 3-5 minutes.

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