Experimental mouse obesity model feed and its preparation method and application

Abstract

The present invention relates to an experimental mouse obesity model feed, a preparation method, and an application thereof, belonging to the technical field of animal model feeds. The feed comprises the following raw materials: casein, sucrose, cellulose, maltodextrin, L-cystine, a mineral mixture, a vitamin mixture, choline bitartrate, lard, soybean oil, pregelatinized starch, sodium cholate, a Chinese herbal extract, and a dye. Advantageously, the present invention overcomes many drawbacks of current obesity modeling feeds, particularly improving the success rate of modeling, shortening the modeling cycle, maximizing the simulation of obesity caused by a high-fat diet in humans, and providing more convenient experimental conditions for human obesity research. Furthermore, the experimental mice are fed using professional and rigorous feeding methods to ensure the scientificity and consistency of the modeling.

Description

Technical Field

[0001] The present invention relates to the technical field of animal model feed, in particular to an experimental mouse obesity model feed and a preparation method and application thereof. Background Art

[0002] Human obesity is often caused by excessive energy intake, leading to fat accumulation. Obesity is a nutritional metabolic disorder caused by a combination of genetic and environmental factors. To develop scientific diagnostic protocols for obesity treatment, animal models of human disease are often used in early theoretical research. These studies often use a high-fat diet to induce obesity in animal models. Animal modeling is a crucial preliminary step in obesity research. Diet-induced animal obesity models, similar to human obesity, are often used to investigate the relationship between factors such as diet and genetics and the progression of diseases like obesity and diabetes.

[0003] The mouse obesity model (DIO) established by feeding a high-calorie diet can serve as a suitable animal model for preclinical drug efficacy studies of obesity and related complications. Commonly used induced obesity models in related technologies include SD rats, Wistar rats, and C57BL / 6J mice. However, high-fat diets currently produced by domestic and international biotechnology companies have unsatisfactory modeling rates and duration. Using a traditional high-fat diet formula (primarily composed of lard and basal diet) to establish an obesity model in rats requires 8-10 weeks to establish a model, while for C57BL / 6J mice, it takes 10-12 weeks. This results in a long experimental period, and the modeling rate rarely reaches 80%. This is characterized by long modeling times, low modeling rates, and low palatability. Furthermore, long-term feeding of a high-fat diet can lead to excessive skin lipid secretion in mice, resulting in seborrheic alopecia and other issues. Currently, laboratories often use a single high-fat diet to establish animal obesity models. However, due to the inherent low palatability of a high-fat diet, continuous feeding can easily lead to anorexia, poor food utilization, and unstable weight gain. Summary of the Invention

[0004] The purpose of the present invention is to provide an experimental mouse obesity model feed in response to the deficiencies in the prior art, so as to solve the problems existing in the related art such as long culture time and low modeling success rate.

[0005] The present invention also provides a method for preparing the above-mentioned experimental mouse obesity model feed.

[0006] The present invention also provides application of the above experimental mouse obesity model feed.

[0007] Specifically, the first aspect of the present invention provides an experimental mouse obesity model feed, comprising the following raw materials by weight:

[0008] Casein: 8%-12%, Sucrose: 10%-15%, Cellulose: 3%-5%, Maltodextrin: 15%-17%, L-cystine: 0.25%-0.4%, Mineral mixture: 6%-7%, Vitamin mixture: 1%-2%, Choline bitartrate: 0.25%-0.3%, Lard: 30%-35%, Soybean oil: 3%-5%, Pregelatinized starch: 5%-8%, Sodium cholate: 0.1%-0.2%, Traditional Chinese Medicine Extract: 2%-4%, Dye: 0.01%-0.02%;

[0009] The Chinese medicine extract is composed of the following preparation raw materials:

[0010] Astragalus, White Peony Root and Codonopsis pilosula.

[0011] According to one technical solution of the feed technical solution of the present invention, at least the following beneficial effects are achieved:

[0012] Casein: Casein is a phosphorus-calcium-binding protein and a protein component. It has the function of regulating blood lipids and can supplement the calcium needed in the body, thereby promoting the growth of mice.

[0013] Sucrose: Sucrose will be hydrolyzed to form glucose, which will participate in metabolism and be absorbed, converted and provided energy by the body.

[0014] Cellulose: Promotes gastrointestinal motility, keeps the intestines unobstructed, and maintains normal food intake.

[0015] Maltodextrin: It has a thickening effect and can plasticize the feed, maintaining the hardness and shape of the feed; it also contains a certain amount of vitamins and trace elements, promoting the body's normal metabolism and renewal.

[0016] L-cystine: As a feed nutritional enhancer, it is beneficial to animal development, increasing body weight and liver and kidney function.

[0017] Mineral mixture: provides mineral elements required for normal growth and development of animals.

[0018] Vitamin mixture: provides vitamins required for normal growth and development of animals.

[0019] Choline bitartrate: widely distributed in the body in the form of phosphate or acetylcholine, involved in phospholipid metabolism, transmission of nerve impulses and preventing the accumulation of abnormal amounts of fat.

[0020] Soybean oil: Rich in unsaturated fatty acids, it enhances lipid metabolism, prevents cardiovascular and cerebrovascular diseases, and has antioxidant and anti-aging effects. It can also improve the negative effects of excessive high-fat, high-calorie diets on mice.

[0021] Lard: Lard has a high digestibility and absorption rate, and is also rich in vitamin A and vitamin D; thus causing mice to gain weight.

[0022] Pregelatinized starch: good thickening effect, strong adhesion, certain water retention properties, and easy digestion and absorption.

[0023] Sodium cholate: helps the body digest and absorb fat, turns fat into extremely small droplets, increases the contact area between fat and enzymes, facilitates the decomposition and absorption of fat, promotes the absorption of vitamins along with the decomposition products of fat, stimulates intestinal peristalsis, and inhibits the growth of intestinal bacteria.

[0024] The Chinese medicinal extract of the invention is prepared from astragalus, white peony root and codonopsis pilosula, wherein the astragalus extract has obvious effects of lowering blood sugar and improving insulin sensitivity, and can improve substance metabolism in the body, thereby promoting weight gain in mice.

[0025] Codonopsis pilosula: Codonopsis pilosula extract can increase the content of gastric wall-bound mucus, play a role in resisting gastric mucosal damage, and can increase the tension of the longitudinal muscles of the gastric fundus and increase the contraction of the circular muscles of the gastric body and gastric antrum.

[0026] White Peony Root: White Peony Root extract can strengthen the spleen and appetite, help digest food and eliminate accumulation; thereby promoting weight gain in mice.

[0027] The Chinese medicinal extract of the present invention can lower blood sugar, improve metabolism of substances in the body, promote gastrointestinal smooth muscle excitation, and further promote gastric juice secretion and gastrointestinal motility, and improve gastric motility deficiency; in addition, it can also improve microcirculation, thereby promoting weight gain in mice.

[0028] The casein, L-cystine, cellulose, mineral mixture, vitamin mixture, and choline bitartrate in the feed of the present invention ensure that the feed provides mice with sufficient protein, amino acids, cellulose, minerals, vitamins, and choline, which is of great significance for maintaining normal growth and development of mice. The addition of lard, soybean oil, and traditional Chinese medicine extracts increases the obesity rate and palatability of the feed.

[0029] The present invention overcomes many drawbacks of current obesity modeling feeds, especially improves the success rate of modeling, shortens the modeling cycle, maximizes the simulation of obesity caused by a high-fat diet in humans, and provides more convenient experimental conditions for the study of human obesity. At the same time, the experimental mice are fed with professional and rigorous feeding methods to ensure the scientificity and consistency of the model.

[0030] According to some embodiments of the present invention, the mineral mixture is composed of the following raw materials in mass fractions:

[0031] Calcium carbonate: 50%-60%, sodium chloride: 25%-26%, potassium chloride: 11%-13%, magnesium sulfate: 5%-6%, iron lactate: 0.4%-0.5%, manganese citrate: 1%-2%, zinc citrate: 1%-2%, sodium fluoride: 0.005%-0.01% and sodium selenite 0.0005%-0.001%.

[0032] According to some embodiments of the present invention, the vitamin mixture is composed of the following raw materials in the following mass fractions:

[0033] Vitamin A: 3%~5%, Vitamin D: 20%~30%, Vitamin E: 40%~60% and Vitamin K: 10%~30%.

[0034] According to some embodiments of the present invention, the raw materials for preparing the experimental mouse obesity model also include water.

[0035] According to some embodiments of the present invention, the mass fraction of water is 2% to 3%.

[0036] According to some embodiments of the present invention, the Chinese herbal medicine extract is composed of the following raw materials in weight fractions:

[0037] Astragalus: 30%~40%, White Peony Root: 10%~20% and Codonopsis: 40%~60%.

[0038] According to some embodiments of the present invention, the method for preparing the Chinese medicine extract comprises the following steps:

[0039] The astragalus root, white peony root and codonopsis root are mixed and extracted with an ethanol aqueous solution with a volume fraction of 50% to 70%, followed by solid-liquid separation, and the liquid phase is collected and concentrated to dryness.

[0040] According to some embodiments of the present invention, the extraction temperature is 60°C to 70°C.

[0041] According to some embodiments of the present invention, the extraction time is 2 hours to 3 hours.

[0042] According to some embodiments of the present invention, the mesh size of the casein is 100-200 mesh.

[0043] According to some embodiments of the present invention, the mesh size of the maltodextrin is 100-200 mesh.

[0044] According to some embodiments of the present invention, the mesh size of the sucrose is 60 mesh to 100 mesh.

[0045] According to some embodiments of the present invention, the dye is a food grade blue dye.

[0046] The second aspect of the present invention discloses a method for preparing the above-mentioned experimental mouse obesity model feed, comprising the following steps:

[0047] mixing the raw materials, granulating and drying;

[0048] The mixing temperature is 20°C to 30°C.

[0049] According to some embodiments of the present invention, the mixing time is 1 h to 2 h.

[0050] According to some embodiments of the present invention, the drying temperature is 20°C to 25°C.

[0051] According to some embodiments of the present invention, the humidity during the drying process is 20% to 30%.

[0052] According to some embodiments of the present invention, the drying time is 24 hours to 36 hours.

[0053] The third aspect of the present invention discloses the use of the above-mentioned experimental mouse obesity model feed in experimental mouse modeling.

[0054] According to some embodiments of the present invention, the modeling time of the experimental mice is 5 to 7 weeks.

[0055] According to some embodiments of the present invention, the breeding density of the experimental mice is 3 to 5 mice per cage. BRIEF DESCRIPTION OF THE DRAWINGS

[0056] Figure 1 Schematic diagram of the mechanism of action of the model feed in an embodiment of the present invention.

[0057] Figure 2 This is a comparison of mice after feeding between Test Example 1 of the present invention and the control group. DETAILED DESCRIPTION

[0058] In order to make the purpose, technical solutions and advantages of this application more clearly understood, the present application is described and illustrated below in conjunction with the accompanying drawings and examples. It should be understood that the specific embodiments described herein are merely used to explain this application and are not intended to limit this application. Based on the embodiments provided in this application, all other embodiments obtained by those of ordinary skill in the art without making any creative effort are within the scope of protection of this application.

[0059] Obviously, the drawings described below are merely examples or embodiments of the present application. Those skilled in the art can, without inventive effort, apply the present application to other similar scenarios based on these drawings. Furthermore, it is also understood that, although the effort involved in such a development process may be complex and lengthy, for those skilled in the art related to the content disclosed in this application, changes in design, manufacturing, or production based on the technical content disclosed in this application are merely conventional technical means and should not be construed as an insufficiency of the content disclosed in this application.

[0060] References to "embodiments" in this application mean that a particular feature, structure, or characteristic described in connection with the embodiment may be included in at least one embodiment of the application. The appearance of this phrase in various places in the specification does not necessarily refer to the same embodiment, nor does it refer to independent or alternative embodiments that are mutually exclusive of other embodiments. It is understood, both explicitly and implicitly, by those skilled in the art that the embodiments described in this application may be combined with other embodiments unless there is a conflict.

[0061] Unless otherwise defined, the technical terms or scientific terms involved in this application should be understood by people with ordinary skills in the technical field to which this application belongs. The "one", "a", "a", "the" and similar words involved in this application do not represent quantitative restrictions and can represent the singular or plural. The terms "include", "comprise", "have" and any of their variations involved in this application are intended to cover non-exclusive inclusions; for example, a process, method, system, product or device that includes a series of steps or units (units) is not limited to the listed steps or units, but may also include steps or units that are not listed, or may also include other steps or units inherent to these processes, methods, products or devices. The terms "first", "second", "third" and the like involved in this application are merely to distinguish similar objects and do not represent a specific ordering of objects.

[0062] In the description of the present invention, reference to terms such as "one embodiment," "some embodiments," "illustrative embodiments," "examples," "specific examples," or "some examples" means that the specific features, structures, materials, or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the exemplary expressions of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0063] If the specific conditions are not specified in the examples, the experiments were carried out under conventional conditions or those recommended by the manufacturer. All reagents or instruments used, if the manufacturer is not specified, are commercially available conventional products.

[0064] The dye selected in the embodiment of the present invention is a food grade blue dye.

[0065] Example 1

[0066] This example is a feed for an experimental mouse obesity model, which is composed of the following raw materials by mass fraction:

[0067] Casein (mesh number 150): 10%, sucrose (mesh number 60): 11%, cellulose: 4%, maltodextrin (mesh number 150): 16%, L-cystine: 0.3%, mineral mixture: 7%, vitamin mixture: 2%, choline bitartrate: 0.3%, lard: 33%, soybean oil: 4%, pregelatinized starch: 6%, sodium cholate: 0.1%, Chinese herbal medicine extract: 4%, dye: 0.01%, water: 2.29%;

[0068] The Chinese herbal medicine extract is composed of the following raw materials in the following weight fractions:

[0069] Astragalus: 35%, White Peony Root: 15% and Codonopsis: 50%.

[0070] The mineral mixture in this embodiment is composed of the following raw materials in weight fractions:

[0071] Calcium carbonate: 54%, sodium chloride: 25%, potassium chloride: 12%, magnesium sulfate: 5.5%, iron lactate: 0.45%, manganese citrate: 1.53%, zinc citrate: 1.51%, sodium fluoride: 0.0092% and sodium selenite 0.0008%.

[0072] The vitamin mixture in this embodiment is composed of the following raw materials in weight fractions:

[0073] Vitamin A: 5%, Vitamin D: 25%, Vitamin E: 50% and Vitamin K: 20%.

[0074] The preparation method of the Chinese herbal medicine extract in this embodiment consists of the following steps:

[0075] Astragalus, white peony root and Codonopsis pilosula were ground (mesh size after grinding was 60 mesh) and mixed; then extracted with 65% ethanol aqueous solution (extraction temperature was 65°C, extraction time was 3h) and solid-liquid separation was performed, the liquid phase was collected and concentrated to dryness.

[0076] The method for preparing the experimental mouse obesity model feed in this embodiment consists of the following steps:

[0077] The raw materials are ground (to 200 mesh), mixed, granulated, and dried;

[0078] The mixing temperature was 25°C and the mixing time was 1.5 h.

[0079] The drying temperature is 20°C, the humidity during the drying process is 20%, and the drying time is 24 hours.

[0080] Example 2

[0081] This example is a feed for an experimental mouse obesity model, which is composed of the following raw materials by mass fraction:

[0082] Casein (mesh number 150): 8%, sucrose (mesh number 60): 10%, cellulose: 5%, maltodextrin (mesh number 150): 16%, L-cystine: 0.3%, mineral mixture: 7%, vitamin mixture: 2%, choline bitartrate: 0.3%, lard: 35%, soybean oil: 5%, pregelatinized starch: 5%, sodium cholate: 0.1%, traditional Chinese medicine extract: 4%, dye: 0.01%, water 2.29%;

[0083] The Chinese herbal medicine extract is composed of the following raw materials in the following weight fractions:

[0084] Astragalus: 30%, White Peony Root: 20% and Codonopsis: 50%.

[0085] The mineral mixture in this embodiment is composed of the following raw materials in weight fractions:

[0086] Calcium carbonate: 54%, sodium chloride: 25%, potassium chloride: 12%, magnesium sulfate: 5.5%, iron lactate: 0.45%, manganese citrate: 1.53%, zinc citrate: 1.51%, sodium fluoride: 0.0092% and sodium selenite 0.0008%.

[0087] The vitamin mixture in this embodiment is composed of the following raw materials in weight fractions:

[0088] Vitamin A: 5%, Vitamin D: 25%, Vitamin E: 50% and Vitamin K: 20%.

[0089] The preparation method of the Chinese herbal medicine extract in this embodiment consists of the following steps:

[0090] Astragalus, white peony root and Codonopsis pilosula were ground (mesh size after grinding was 60 mesh) and mixed; then extracted with 65% ethanol aqueous solution (extraction temperature was 65°C, extraction time was 3h) and solid-liquid separation was performed, the liquid phase was collected and concentrated to dryness.

[0091] The method for preparing the experimental mouse obesity model feed in this example is carried out with reference to Example 1.

[0092] Example 3

[0093] This example is a feed for an experimental mouse obesity model, which is composed of the following raw materials by mass fraction:

[0094] Casein (mesh number 150): 11.6%, sucrose (mesh number 60): 11.8%, cellulose: 4%, maltodextrin (mesh number 150): 17%, L-cystine: 0.3%, mineral mixture: 6.5%, vitamin mixture: 2%, choline bitartrate: 0.3%, lard: 32%, soybean oil: 4%, pregelatinized starch: 6%, sodium cholate: 0.2%, traditional Chinese medicine extract: 2%, dye: 0.01%, water 2.29%;

[0095] The Chinese herbal medicine extract is composed of the following raw materials in the following weight fractions:

[0096] Astragalus: 30%, White Peony Root: 30% and Codonopsis: 40%.

[0097] The mineral mixture in this embodiment is composed of the following raw materials in weight fractions:

[0098] Calcium carbonate: 54%, sodium chloride: 25%, potassium chloride: 12%, magnesium sulfate: 5.5%, iron lactate: 0.45%, manganese citrate: 1.53%, zinc citrate: 1.51%, sodium fluoride: 0.0092% and sodium selenite 0.0008%.

[0099] The vitamin mixture in this embodiment is composed of the following raw materials in weight fractions:

[0100] Vitamin A: 5%, Vitamin D: 20%, Vitamin E: 55% and Vitamin K: 20%.

[0101] The preparation method of the Chinese herbal medicine extract in this embodiment consists of the following steps:

[0102] Astragalus, white peony root and Codonopsis pilosula were ground (mesh size after grinding was 60 mesh) and mixed; then extracted with 65% ethanol aqueous solution (extraction temperature was 65°C, extraction time was 3h) and solid-liquid separation was performed, the liquid phase was collected and concentrated to dryness.

[0103] The method for preparing the experimental mouse obesity model feed in this example is carried out with reference to Example 1.

[0104] Example 4

[0105] This example is a feed for an experimental mouse obesity model, which is composed of the following raw materials by mass fraction:

[0106] Casein (mesh number 150): 8%, sucrose (mesh number 60): 15%, cellulose: 4.8%, maltodextrin (mesh number 150): 17%, L-cystine: 0.4%, mineral mixture: 6.1%, vitamin mixture: 2%, choline bitartrate: 0.25%, lard: 30%, soybean oil: 4%, pregelatinized starch: 6%, sodium cholate: 0.15%, Chinese herbal medicine extract: 4%, dye: 0.01%, water: 2.29%;

[0107] The Chinese herbal medicine extract is composed of the following raw materials in the following weight fractions:

[0108] Astragalus: 40%, White Peony Root: 20% and Codonopsis: 40%.

[0109] The mineral mixture in this embodiment is composed of the following raw materials in weight fractions:

[0110] Calcium carbonate: 54%, sodium chloride: 25%, potassium chloride: 12%, magnesium sulfate: 5.5%, iron lactate: 0.45%, manganese citrate: 1.53%, zinc citrate: 1.51%, sodium fluoride: 0.0092% and sodium selenite 0.0008%.

[0111] The vitamin mixture in this embodiment is composed of the following raw materials in weight fractions:

[0112] Vitamin A: 5%, Vitamin D: 20%, Vitamin E: 45% and Vitamin K: 30%.

[0113] The preparation method of the Chinese herbal medicine extract in this embodiment consists of the following steps:

[0114] Astragalus, white peony root and Codonopsis pilosula were ground (mesh size after grinding was 60 mesh) and mixed; then extracted with 65% ethanol aqueous solution (extraction temperature was 65°C, extraction time was 3h) and solid-liquid separation was performed, the liquid phase was collected and concentrated to dryness.

[0115] The method for preparing the experimental mouse obesity model feed in this example is carried out with reference to Example 1.

[0116] Example 5

[0117] This example is a feed for an experimental mouse obesity model, which is composed of the following raw materials by mass fraction:

[0118] Casein (mesh number 150): 12%, sucrose (mesh number 60): 14%, cellulose: 4%, maltodextrin (mesh number 150): 16%, L-cystine: 0.3%, mineral mixture: 6%, vitamin mixture: 2%, choline bitartrate: 0.3%, lard: 30%, soybean oil: 4%, pregelatinized starch: 6%, sodium cholate: 0.1%, Chinese herbal medicine extract: 3%, dye: 0.01%, water: 2.29%;

[0119] The Chinese herbal medicine extract is composed of the following raw materials in the following weight fractions:

[0120] Astragalus: 38%, White Peony Root: 12% and Codonopsis: 50%.

[0121] The mineral mixture in this embodiment is composed of the following raw materials in weight fractions:

[0122] Calcium carbonate: 54%, sodium chloride: 25%, potassium chloride: 12%, magnesium sulfate: 5.5%, iron lactate: 0.45%, manganese citrate: 1.53%, zinc citrate: 1.51%, sodium fluoride: 0.0092% and sodium selenite 0.0008%.

[0123] The vitamin mixture in this embodiment is composed of the following raw materials in weight fractions:

[0124] Vitamin A: 5%, Vitamin D: 28%, Vitamin E: 47% and Vitamin K: 20%.

[0125] The preparation method of the Chinese herbal medicine extract in this embodiment consists of the following steps:

[0126] Astragalus, white peony root and Codonopsis pilosula were ground (mesh size after grinding was 60 mesh) and mixed; then extracted with 65% ethanol aqueous solution (extraction temperature was 65°C, extraction time was 3h) and solid-liquid separation was performed, the liquid phase was collected and concentrated to dryness.

[0127] The method for preparing the experimental mouse obesity model feed in this example is carried out with reference to Example 1.

[0128] Comparative Example 1

[0129] This comparative example is a feed for an experimental mouse obesity model, which is composed of the following raw materials by mass fraction:

[0130] Casein (mesh number 150): 10%, sucrose (mesh number 60): 11%, cellulose: 4%, maltodextrin (mesh number 150): 16%, L-cystine: 0.3%, mineral mixture: 7%, vitamin mixture: 2%, choline bitartrate: 0.3%, lard: 33%, soybean oil: 4%, pregelatinized starch: 6%, sodium cholate: 0.1%, traditional Chinese medicine composition: 4%, dye: 0.01%, water: 2.29%;

[0131] The Chinese medicine composition is composed of the following raw materials in weight fractions:

[0132] Astragalus: 35%, White Peony Root: 15% and Codonopsis: 50%.

[0133] The mineral mixture in this comparative example is composed of the following raw materials by weight:

[0134] Calcium carbonate: 54%, sodium chloride: 25%, potassium chloride: 12%, magnesium sulfate: 5.5%, iron lactate: 0.45%, manganese citrate: 1.53%, zinc citrate: 1.51%, sodium fluoride: 0.0092% and sodium selenite 0.0008%.

[0135] The vitamin mixture in this comparative example is composed of the following raw materials in weight fractions:

[0136] Vitamin A: 5%, Vitamin D: 25%, Vitamin E: 50% and Vitamin K: 20%.

[0137] The preparation method of the Chinese medicine composition in this comparative example consists of the following steps:

[0138] Grind astragalus, white peony root and codonopsis pilosula (mesh size after grinding is 60 meshes) and mix them to obtain the product.

[0139] The preparation method of the experimental mouse obesity model feed in this comparative example was carried out with reference to Example 1.

[0140] Comparative Example 2

[0141] This comparative example is a feed for an experimental mouse obesity model, which is composed of the following raw materials by mass fraction:

[0142] Casein (mesh number 150): 10%, sucrose (mesh number 60): 11%, cellulose: 4%, maltodextrin (mesh number 150): 16%, L-cystine: 0.3%, mineral mixture: 7%, vitamin mixture: 2%, choline bitartrate: 0.3%, lard: 33%, soybean oil: 4%, pregelatinized starch: 6%, sodium cholate: 0.1%, Chinese herbal medicine extract: 4%, dye: 0.01%, water: 2.29%;

[0143] The Chinese herbal medicine extract is composed of the following raw materials in the following weight fractions:

[0144] Astragalus: 50% and Codonopsis: 50%.

[0145] The mineral mixture in this comparative example is composed of the following raw materials by weight:

[0146] Calcium carbonate: 54%, sodium chloride: 25%, potassium chloride: 12%, magnesium sulfate: 5.5%, iron lactate: 0.45%, manganese citrate: 1.53%, zinc citrate: 1.51%, sodium fluoride: 0.0092% and sodium selenite 0.0008%.

[0147] The vitamin mixture in this comparative example is composed of the following raw materials in weight fractions:

[0148] Vitamin A: 5%, Vitamin D: 25%, Vitamin E: 50% and Vitamin K: 20%.

[0149] The preparation method of the Chinese herbal medicine extract in this comparative example consists of the following steps:

[0150] Astragalus and Codonopsis pilosula were ground (mesh size after grinding was 60 mesh) and mixed; then extracted with 65% ethanol aqueous solution (extraction temperature was 65° C., extraction time was 3 h) and solid-liquid separation was performed, the liquid phase was collected and concentrated to dryness.

[0151] The preparation method of the experimental mouse obesity model feed in this comparative example was carried out with reference to Example 1.

[0152] Comparative Example 3

[0153] This comparative example is a feed for an experimental mouse obesity model, which is composed of the following raw materials by mass fraction:

[0154] Casein (mesh number 150): 10%, sucrose (mesh number 60): 11%, cellulose: 4%, maltodextrin (mesh number 150): 16%, L-cystine: 0.3%, mineral mixture: 7%, vitamin mixture: 2%, choline bitartrate: 0.3%, lard: 33%, soybean oil: 4%, pregelatinized starch: 6%, sodium cholate: 0.1%, Chinese herbal medicine extract (Codonopsis pilosula extract): 4%, dye: 0.01%, water: 2.29%;

[0155] The mineral mixture in this comparative example is composed of the following raw materials by weight:

[0156] Calcium carbonate: 54%, sodium chloride: 25%, potassium chloride: 12%, magnesium sulfate: 5.5%, iron lactate: 0.45%, manganese citrate: 1.53%, zinc citrate: 1.51%, sodium fluoride: 0.0092% and sodium selenite 0.0008%.

[0157] The vitamin mixture in this comparative example is composed of the following raw materials in weight fractions:

[0158] Vitamin A: 5%, Vitamin D: 25%, Vitamin E: 50% and Vitamin K: 20%.

[0159] The preparation method of the Chinese herbal medicine extract in this comparative example consists of the following steps:

[0160] The codonopsis pilosula was ground (mesh size after grinding was 60 mesh) and mixed; then extracted with 65% ethanol aqueous solution (extraction temperature was 65° C., extraction time was 3 h) and solid-liquid separation was performed, the liquid phase was collected and concentrated to dryness.

[0161] The preparation method of the experimental mouse obesity model feed in this comparative example was carried out with reference to Example 1.

[0162] Comparative Example 4

[0163] This comparative example is a feed for an experimental mouse obesity model, which is composed of the following raw materials by mass fraction:

[0164] Casein (mesh number 150): 10%, sucrose (mesh number 60): 11%, cellulose: 4%, maltodextrin (mesh number 150): 16%, L-cystine: 0.3%, mineral mixture: 7%, vitamin mixture: 2%, choline bitartrate: 0.3%, lard: 33%, soybean oil: 4%, pregelatinized starch: 6%, sodium cholate: 0.1%, dye: 0.01%, water: 6.29%;

[0165] The mineral mixture in this comparative example is composed of the following raw materials by weight:

[0166] Calcium carbonate: 54%, sodium chloride: 25%, potassium chloride: 12%, magnesium sulfate: 5.5%, iron lactate: 0.45%, manganese citrate: 1.53%, zinc citrate: 1.51%, sodium fluoride: 0.0092% and sodium selenite 0.0008%.

[0167] The vitamin mixture in this comparative example is composed of the following raw materials in weight fractions:

[0168] Vitamin A: 5%, Vitamin D: 25%, Vitamin E: 50% and Vitamin K: 20%.

[0169] The preparation method of the experimental mouse obesity model feed in this comparative example was carried out with reference to Example 1.

[0170] Comparative Example 5

[0171] This comparative example is a feed for an experimental mouse obesity model, which is composed of the following raw materials by mass fraction:

[0172] Casein (mesh number 150): 10%, sucrose (mesh number 60): 11%, cellulose: 4%, maltodextrin (mesh number 150): 16%, L-cystine: 0.3%, mineral mixture: 7%, vitamin mixture: 2%, choline bitartrate: 0.3%, lard: 33%, soybean oil: 4%, pregelatinized starch: 6%, Chinese herbal medicine extract: 4%, dye: 0.01%, water: 2.39%;

[0173] The Chinese herbal medicine extract is composed of the following raw materials in the following weight fractions:

[0174] Astragalus: 35%, White Peony Root: 15% and Codonopsis: 50%.

[0175] The mineral mixture in this comparative example is composed of the following raw materials by weight:

[0176] Calcium carbonate: 54%, sodium chloride: 25%, potassium chloride: 12%, magnesium sulfate: 5.5%, iron lactate: 0.45%, manganese citrate: 1.53%, zinc citrate: 1.51%, sodium fluoride: 0.0092% and sodium selenite 0.0008%.

[0177] The vitamin mixture in this comparative example is composed of the following raw materials in weight fractions:

[0178] Vitamin A: 5%, Vitamin D: 25%, Vitamin E: 50% and Vitamin K: 20%.

[0179] The preparation method of the Chinese herbal medicine extract in this comparative example consists of the following steps:

[0180] Astragalus, white peony root and Codonopsis pilosula were ground (mesh size after grinding was 60 mesh) and mixed; then extracted with 65% ethanol aqueous solution (extraction temperature was 65°C, extraction time was 3h) and solid-liquid separation was performed, the liquid phase was collected and concentrated to dryness.

[0181] The preparation method of the experimental mouse obesity model feed in this comparative example was carried out with reference to Example 1.

[0182] Test example:

[0183] Experimental animals:

[0184] One hundred and ten 6-week-old C57BL / 6J male mice weighing approximately 20.00 g were purchased and selected.

[0185] Group settings:

[0186] Experimental group 1: the mice were fed with the feed prepared in Example 1 of the present invention.

[0187] Experimental group 2: the mice were fed with the feed prepared in Example 2 of the present invention.

[0188] Experimental group 3: the mice were fed with the feed prepared in Example 3 of the present invention.

[0189] Experimental group 4: the mice were fed with the feed prepared in Example 4 of the present invention.

[0190] Experimental group 5: The mice were fed with the feed prepared in Example 5 of the present invention.

[0191] Experimental group 6: the mice were fed with the feed in comparative example 1 of the present invention.

[0192] Experimental group 7: the feed in comparative example 2 of the present invention was fed.

[0193] Experimental group 8: fed with the feed in comparative example 3 of the present invention.

[0194] Experimental group 9: fed with the feed in comparative example 4 of the present invention.

[0195] Experimental group 10: fed with the feed in comparative example 5 of the present invention.

[0196] Control group: fed with ordinary feed (Jiangsu Collaborative Pharmaceutical Bioengineering Co., Ltd., product number: XTCON50J).

[0197] The number of mice in each group was 10.

[0198] The parameters during the feeding process of the mice in the experimental group of the present invention were controlled as follows:

[0199] Each group of mice had free access to food and water every day, the room temperature was maintained at 20℃~22℃, the humidity was 60%±2%, and the light duration was 12h±1h; the breeding time was 6 weeks.

[0200] After 6 weeks, the body weight, Lee index, body length and body fat (TC, HDL, LDL) of each mouse were tested; the specific test data are shown in Table 1.

[0201] Table 1 Data on weight, Lee index, body length and body fat of mice in the test examples of the present invention

[0202]

[0203]

[0204]

[0205] At the end of the sixth week, the average weight gain in the experimental groups 1 to 5 was at least about 17 g (see the mechanism of action of the experimental group feed in the present invention). Figure 1); the average weight gain of the experimental group 6 to 10 was about 15g; the weight gain of the control group was only about 4g (see the comparison chart of experimental group 1 and control group Figure 2 ( Figure 2 The mice on the left are experimental group 1, and the mice on the right are the control group (the mice in the control group are in a curled-up state and not fully stretched out). The three blood lipid items (TC, HDL, LDL) of experimental groups 1 to 5 are significantly different from those of experimental groups 6 to 10; that is, the feeds in Examples 1 to 5 of the present invention are more likely to promote obesity in mice.

[0206] The advantage of the present invention is that the present invention uses a scientific and reasonable unique raw material ratio to crush, mix and granulate, and then the primary product is dried under a low temperature and low humidity environment to obtain feed. After feeding mice with this high-fat feed for 6 weeks, the model indicators of high body weight, high body fat rate, local excessive fat deposition, high level of triglycerides in the blood, and high level of total cholesterol content in mice can be obtained; the present invention scientifically feeds experimental mice, avoids the occurrence of non-compliant feeding time and amount due to human manipulation, and is beneficial to the integrity and consistency of the model, improves the success rate and efficiency of modeling, simulates various symptoms of human obesity, and provides good experimental conditions for the study of human related diseases. The present invention overcomes many drawbacks of current obesity modeling feeds, especially improves the success rate of modeling, shortens the modeling cycle, maximizes the simulation of obesity caused by human high-fat diet, and provides more convenient prerequisite experimental conditions for the study of human obesity. At the same time, the experimental mice are fed with professional and rigorous feeding methods to ensure the scientificity and consistency of the model.

[0207] The above description is only a preferred embodiment of the present invention and does not limit the implementation mode and protection scope of the present invention. For those skilled in the art, it should be aware that all solutions obtained by equivalent substitutions and obvious changes made using the description and illustrations of the present invention should be included in the protection scope of the present invention.

Claims

1. A feed for an experimental mouse obesity model, characterized in that: The preparation comprises the following raw materials in weight fractions: Casein: 8%-12%, Sucrose: 10%-15%, Cellulose: 3%-5%, Maltodextrin: 15%-17%, L-cystine: 0.25%-0.4%, Mineral mixture: 6%-7%, Vitamin mixture: 1%-2%, Choline bitartrate: 0.25%-0.3%, Lard: 30%-35%, Soybean oil: 3%-5%, Pregelatinized starch: 5%-8%, Sodium cholate: 0.1%-0.2%, Traditional Chinese Medicine Extract: 2%-4%, Dye: 0.01%-0.02%; The Chinese herbal medicine extract is composed of the following preparation materials: astragalus root, white peony root and codonopsis root; The mineral mixture is composed of the following raw materials in mass fractions: calcium carbonate: 50% to 60%, sodium chloride: 25% to 26%, potassium chloride: 11% to 13%, magnesium sulfate: 5% to 6%, iron lactate: 0.4% to 0.5%, manganese citrate: 1% to 2%, zinc citrate: 1% to 2%, sodium fluoride: 0.005% to 0.01% and sodium selenite 0.0005% to 0.001%; The vitamin mixture is composed of the following raw materials in mass fractions: vitamin A: 3% to 5%, vitamin D: 20% to 30%, vitamin E: 40% to 60% and vitamin K: 10% to 30%; The Chinese herbal medicine extract is composed of the following raw materials in weight fractions: Astragalus root: 30% to 40%, White Peony root: 10% to 20% and Codonopsis root: 40% to 60%; The preparation method of the traditional Chinese medicine extract comprises the following steps: mixing the astragalus root, white peony root and codonopsis root, extracting with an ethanol aqueous solution with a volume fraction of 50% to 70%, separating the solid and liquid, collecting the liquid phase and concentrating to dryness; the extraction temperature is 60° C. to 70° C.

2. The experimental mouse obesity model feed according to claim 1, characterized in that The raw materials for preparing the experimental mouse obesity model also include water.

3. A method for preparing an experimental mouse obesity model feed according to claim 1 or 2, characterized in that: The following steps are involved: mixing the raw materials, granulating and drying; The mixing temperature is 20°C to 30°C.

4. Use of the experimental mouse obesity model feed according to any one of claims 1 to 3 in experimental mouse modeling.

5. A use according to claim 4, characterized in that The modeling time of the experimental mice is 5 to 7 weeks.

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