A skin barrier repair cream and its preparation method

Abstract

This application relates to the technical field of cosmetics, and specifically discloses a skin barrier repair cream and a preparation method thereof. The skin barrier repair cream of this application comprises the following raw materials in parts by weight: 11-15 parts of plant ferment, 1-3 parts of water lily extract, 0.8-1.6 parts of buddleja officinalis extract, 1.2-2.4 parts of peony flower extract, 0.6-1.1 parts of cetearyl olivate, 0.1-0.5 parts of sorbitan olivate, 0.2-0.8 parts of tocopheryl acetate, 0.6-1.2 parts of hydrolyzed sclerotium gum, 1-2 parts of carbomer, 1-2 parts of betaine, 2-4 parts of hyaluronic acid, 0.5-0.9 parts of glycerin, 0.1-0.15 parts of fumed silica, 0.2-0.3 parts of montmorillonite, and 15-30 parts of water; In the above solution, the formula is simple and the proportion is rigorous. The components interact with each other to effectively repair the skin barrier, protect cell vitality, enable the skin to have the ability to resist external environmental factors, and have broad market prospects.

Description

Technical Field

[0001] The present application relates to the technical field of cosmetics, and more specifically, it relates to a skin barrier repair cream and a preparation method thereof. Background Art

[0002] With the acceleration of the social life rhythm, the aggravation of urban environmental pollution, the endocrine disorders caused by coping with internal and external pressures, the weakening of the skin with age, etc., the skin becomes dull and spotty; the skin elasticity decreases, the skin is not plump, dry and peeling; the skin appears itchy and sensitive, etc. To solve various skin problems, it is necessary to use a variety of skin care products with different functions, which will easily cause eutrophication of the skin, increase the burden on the skin, and only make the skin more sensitive, with decreased resistance and serious damage to the skin barrier. When the skin barrier is damaged, the normal protective function of the skin surface is lost, resulting in the loss of skin moisture, and thus the skin becomes dry, peeling or itchy; at the same time, the germs on the skin surface easily enter the skin through the damaged skin barrier, further triggering skin inflammatory reactions.

[0003] At present, although many skin care products on the market have a certain function of repairing the skin barrier, the repair time is long and the effect is slow, and the actual effect is not ideal, and the effect of quickly repairing the damaged skin barrier cannot be achieved. Therefore, there is an urgent need to propose a skin barrier repair cream, which has an outstanding effect of repairing the skin barrier, not only helps to improve the skin condition, but also can prevent and treat skin diseases, which has positive significance. Summary of the Invention

[0004] In order to solve the problem of poor skin barrier repair effect of existing skin care products, the present application provides a skin barrier repair cream and a preparation method thereof.

[0005] In the first aspect, the present application provides a skin barrier repair cream, adopting the following technical solution:

[0006] A skin barrier repair cream, comprising the following raw materials in parts by weight: 11-15 parts of plant ferment, 1-3 parts of water lily extract, 0.8-1.6 parts of buddleja officinalis extract, 1.2-2.4 parts of peony flower extract, 0.6-1.1 parts of cetearyl olivate, 0.1-0.5 parts of sorbitan olivate, 0.6-1.2 parts of hydrolyzed sclerotium gum, 1-2 parts of carbomer, 1-2 parts of betaine, 2-4 parts of hyaluronic acid, 0.5-0.9 parts of glycerol, 15-30 parts of water;

[0007] The plant ferment is obtained by mixing and fermenting a plant mixture with a fermentation strain;

[0008] The plant mixture is obtained by mixing selaginella tamariscina, white willow bark and spring elm root in a mass ratio of 1:2-3:3-4.

[0009] By adopting the above technical solutions, the main active ingredients in the water lily extract include: ellagic acid, isoquercitrin, gallic acid, prunasin, astragalin, and afzelin, which have beneficial skin care effects such as delaying skin aging, anti-skin oxidation, and wrinkle removal; the Buddleja officinalis extract contains various active ingredients of flavonoid compounds, which can inhibit the generation of reactive oxygen species, scavenge free radicals, and prevent skin inflammation by inhibiting cyclooxygenase-2, 5-lipoxygenase, and UVB-induced TNFα, inhibit melanin deposition, and protect the skin from the harm of blue light and ultraviolet radiation; the peony flower extract has functions of activating the skin and anti-aging, such as inhibiting elastase, scavenging free radicals, inhibiting the generation of nitrogen oxides, and promoting the activity of cathepsin, and has good moisturizing ability.

[0010] In the raw materials of the present application, there are plant ferment, water lily extract, Buddleja officinalis extract, and peony flower extract. There is a synergistic effect among the four, interacting and assisting with each other, making the cream have a good effect on repairing the skin barrier and a high repair efficiency; at the same time, raw materials such as cetearyl olivate, sorbitan olivate, hydrolyzed sclerotium gum, carbomer, betaine, hyaluronic acid, and glycerol are also added to improve the permeability of the cream, which is easily absorbed by the skin, promote the effective ingredients to enter the deep layer of the skin, repair the skin, and form a protective barrier on the surface of the skin.

[0011] In addition, the plant ferment of the present application is obtained by mixing and fermenting a plant mixture with a fermentation strain; after the plant mixture is fermented, the active ingredients are further decomposed into small molecules, which is beneficial to the active ingredients of the plant ferment to stimulate skin immunity, strengthen stress tolerance, and enable the skin to rebuild the ability to resist external stimuli.

[0012] In addition, Selaginella tamariscina contains rich polysaccharides, amino acids, proteins, and flavonoid natural skin care factors, which have strong moisturizing, antioxidant, anti-inflammatory, and skin repair effects; white willow bark not only has excellent anti-aging and anti-wrinkle effects, but also has high anti-inflammatory activity, and can effectively relieve facial acne, herpes inflammation, and sunburn; the extract of the root of Ulmus davidiana var. japonica is rich in various polysaccharides, which can inhibit the activity of hyaluronidase, reduce transdermal water loss, and the flavonoid active substances can repair the photo-damage caused by ultraviolet radiation, improve the cell survival rate, and can also inhibit the release of anti-inflammatory factors; at the same time, the extract of the root of Ulmus davidiana var. japonica also has a good repair effect on dry, rough, and fine-line skin, and improves the gloss of the skin; the plant mixture of the present application is obtained from Selaginella tamariscina, white willow bark, and the root of Ulmus davidiana var. japonica in a specific mass ratio, and the three promote each other, synergistically enhance the effect, and the effect of improving the skin repair of the plant ferment is more significant.

[0013] Preferably, the plant ferment is prepared by the following method:

[0014] (1) Crush the plant mixture and mix it with purified water, then perform enzymatic treatment with a composite enzyme. After enzymatic treatment, sterilize to obtain the fermentation broth to be fermented;

[0015] (2) Add fermentation bacteria and liquid medium to the fermentation broth to be fermented, ferment, then sterilize and centrifuge, and take the supernatant to obtain the primary product;

[0016] (3) Add β-cyclodextrin to the primary product, raise the temperature and stir for a period of time, then concentrate under reduced pressure and dry to obtain the plant ferment.

[0017] By adopting the above technical scheme, the plant mixture in this application is first enzymatically hydrolyzed with a composite enzyme, then fermented with fermentation bacteria, and finally included with β-cyclodextrin to obtain a plant ferment with stable performance, which contains rich effective ingredients for repairing the skin.

[0018] Preferably, the mass ratio of the plant mixture, purified water, and composite enzyme is 1:15 - 25:0.06 - 0.1.

[0019] Preferably, the composite enzyme is obtained by mixing papain and snail enzyme with a mass ratio of 3 - 6:5.

[0020] Preferably, the pH of the enzymatic hydrolysis in step (1) is 5 - 6, the temperature is 30 - 50 °C, and the time is 1 - 3 h.

[0021] By adopting the above technical scheme, this application controls the enzymatic hydrolysis conditions in step (1) and the mass ratio of the plant mixture, purified water, and composite enzyme. On the one hand, the effective ingredients in the plant mixture are fully extracted, and on the other hand, it has the positive effects of saving resources and reducing production costs; in addition, the composite enzyme is obtained by mixing papain and snail enzyme in a specific ratio, which can fully extract the effective ingredients of the plant mixture and maintain the biological activity of the effective ingredients at the same time.

[0022] Preferably, the mass ratio of the plant mixture, fermentation bacteria, and liquid medium is 100:7 - 14:30 - 60.

[0023] Preferably, the fermentation bacteria are obtained by mixing Lactobacillus plantarum, yeast, and Acetobacter with a mass ratio of 2 - 6:1 - 5:3 - 7.

[0024] Preferably, the liquid medium is prepared by the following method:

[0025] By weight, mix 3 - 5 parts of melibiose, 1 - 4 parts of mannan oligosaccharide, 0.5 part of peptone, 1 part of maltose, 0.03 part of urea, 0.02 part of diammonium hydrogen phosphate, 0.01 part of sophorolipid, 30 parts of magnesium sulfate, and 30 - 40 parts of water.

[0026] Preferably, the pH value of the fermentation in step (2) is 4.5 - 6.5, the temperature is 25 - 35 °C, and the time is 30 - 50 h.

[0027] By adopting the above technical solution, the present application controls the fermentation conditions in step (2) and the mass ratio of the plant mixture, the fermentation bacteria and the liquid medium, so that the fermentation effect of the plant mixture is better; at the same time, the fermentation bacteria are obtained by mixing Lactobacillus plantarum, yeast and Acetobacter aceti in a specific mass ratio. By using three different strains of bacteria to assist each other, the macromolecules in the plant mixture are fully degraded, and more active ingredients can be obtained to a great extent, which has the effects of moisturizing, repairing the basal layer, and regulating the skin microecology.

[0028] Preferably, the mass of the β-cyclodextrin is 5 - 10% of the initial product.

[0029] Preferably, the stirring temperature in step (3) is 40 - 60 °C, the stirring speed is 400 - 600 r / min, and the stirring time is 2 - 3 h.

[0030] Preferably, the temperature of the vacuum concentration is 55 - 70 °C, the pressure is -0.08 - (-0.01) MPa, and the concentration is up to a relative density of 1.21 - 1.28.

[0031] By adopting the above technical solution, the initial product is included by the β-cyclodextrin with a hollow structure, which can effectively improve the stability of the active ingredients in the plant ferment, prevent the oxidation and decomposition of the active ingredients, and at the same time is also conducive to the better fusion of the plant ferment and other components, improving the comprehensive performance of the skin repair barrier cream.

[0032] Preferably, the skin repair barrier cream further comprises the raw materials in parts by weight: 0.1 - 0.15 parts of fumed silica and 0.2 - 0.3 parts of montmorillonite.

[0033] By adopting the above technical solution, the raw materials of the skin repair barrier cream of the present application further include fumed silica and montmorillonite. Fumed silica is beneficial to improving the dispersibility and compatibility of each component; montmorillonite can prolong the action time of each component on the skin surface and form a mucosal protective barrier on the skin surface to reduce skin moisture evaporation; montmorillonite can also remove cutin and harmful bacteria on the skin surface and accelerate the shedding of aging cells, having the effects of shrinking pores and fading melanin; therefore, the addition of fumed silica and montmorillonite makes the performance of the skin repair barrier cream more excellent.

[0034] In the second aspect, the present application provides a preparation method of a skin repair barrier cream, adopting the following technical solution:

[0035] A preparation method of a skin repair barrier cream includes the following steps:

[0036] S1. After thoroughly grinding fumed silica and montmorillonite, perform plasma treatment to obtain a powder.

[0037] S2. Heat cetearyl olivate, sorbitan olivate, and tocopheryl acetate to 80 - 85 °C, stir to dissolve, obtain mixture A, and keep it warm for standby.

[0038] S3. Heat water, glycerin, hydrolyzed sclerotium gum, carbomer, betaine, and hyaluronic acid to 85 - 90 °C until completely dissolved, obtain mixture B, and keep it warm for standby.

[0039] S4. Slowly add mixture A to mixture B, homogenize and emulsify for 5 - 10 min, then cool down to 60 - 70 °C, add the powder, homogenize for 3 - 5 min, continue to cool down to 40 - 50 °C, add the plant ferment, water lily extract, buddleja flower extract, and peony flower extract, stir until uniform, and sterilize with steam for 5 - 8 min to obtain the skin barrier repair cream.

[0040] By adopting the above technical solution, in this application, fumed silica and montmorillonite are subjected to plasma treatment, increasing the active groups on the surfaces of fumed silica and montmorillonite, promoting fumed silica and montmorillonite to combine with other components by chemical bonds, and strengthening the connection between components. At the same time, after mixtures A and B are mixed, fumed silica and montmorillonite with different microstructures synergistically enhance each other. They can not only adsorb on the dispersed phase interface but also have a certain wrapping effect on the internal phase, improving the stability of the emulsion system. In addition, by controlling each process parameter, each component interacts with and promotes each other, making the skin smooth, delicate, tender, and elastic, repairing fine lines, and achieving the effects of beauty and anti - aging.

[0041] In summary, this application has the following beneficial effects:

[0042] 1. The skin barrier repair cream of this application includes components such as plant ferment, water lily extract, buddleja flower extract, peony flower extract, cetearyl olivate, sorbitan olivate, tocopheryl acetate, hydrolyzed sclerotium gum, carbomer, betaine, and hyaluronic acid. These components interact with each other, and the obtained cream has a significant skin barrier repair effect.

[0043] 2. The preparation method of the plant ferment in this application uses selaginella, white willow bark, and spring elm root as raw materials. First, use a composite enzyme for treatment to remove fiber and pectin in the plants; then ferment with fermenting bacteria, and finally perform inclusion with β - cyclodextrin and vacuum concentration to obtain the plant ferment. No organic solvents are added throughout the process, which is safe and non - toxic. The obtained plant ferment has rich active ingredients, and the skin care effect has been greatly improved.

[0044] 3. The skin barrier repair cream of the present application further includes fumed silica and montmorillonite, which further improves the skin repair efficacy of the cream, forms a protective barrier on the skin surface, and has positive practical significance.

[0045] 4. The preparation method of the skin barrier repair cream of the present application has simple steps and low cost. The obtained cream has remarkable effects such as repairing the skin barrier, whitening and brightening, antioxidant, moisturizing, and soothing inflammation, and has broad market prospects. Specific Embodiments

[0046] The following further elaborates on the present application in conjunction with examples.

[0047] Preparation Examples 1-5 and Comparative Preparation Examples 1-16 provide the preparation methods of plant fermentates.

[0048] Preparation Example 1

[0049] The plant fermentate is prepared by the following method:

[0050] (1) After crushing 100 g of the plant mixture, it is mixed with 1500 g of purified water, and 6 g of a composite enzyme is used for enzymatic treatment. The pH of the enzymatic hydrolysis is 5, the temperature is 30 °C, and the time is 3 h; after enzymatic treatment, sterilization is carried out to obtain the fermentation broth to be fermented;

[0051] (2) 7 g of fermenting bacteria and 30 g of liquid medium are added to the fermentation broth to be fermented. The pH value is adjusted to 4.5, the fermentation temperature is 25 °C, and the fermentation time is 50 h. Subsequently, sterilization is carried out, centrifugation is carried out at a centrifugation speed of 8000 r / min for 45 min, and the supernatant is taken to obtain the primary product;

[0052] (3) 5% of the mass of the primary product is added to the primary product as β-cyclodextrin, and it is stirred at a rotation speed of 400 r / min at 40 °C for 2 h; subsequently, under 55 °C and -0.08 MPa, vacuum concentration is carried out to a relative density of 1.21, and drying is carried out to obtain the plant fermentate;

[0053] Among them, the plant mixture is obtained by mixing selaginella tamariscina, white willow bark, and spring elm root in a mass ratio of 1:2:3; the composite enzyme is obtained by mixing papain and snail enzyme in a mass ratio of 3:5; the fermenting bacteria are obtained by mixing lactobacillus plantarum, yeast, and acetic acid bacteria in a mass ratio of 2:1:3; the liquid medium is obtained by mixing 3 parts of melibiose, 1 g of mannan oligosaccharide, 0.5 g of peptone, 1 g of maltose, 0.03 g of urea, 0.02 g of diammonium hydrogen phosphate, 0.01 g of sophorolipid, 30 g of magnesium sulfate, and 30 g of water.

[0054] Preparation Example 2

[0055] The plant fermentate is prepared by the following method:

[0056] (1) After crushing 100 g of the plant mixture, it is mixed with 1800 g of purified water, and 7 g of a composite enzyme is used for enzymatic treatment. The pH of the enzymatic hydrolysis is 5.3, the temperature is 35 °C, and the time is 2.5 h; after the enzymatic treatment, sterilization is carried out to obtain the fermentation broth to be fermented;

[0057] (2) 8 g of fermentation bacteria and 35 g of liquid medium are added to the fermentation broth to be fermented, the pH value is adjusted to 5, the fermentation temperature is 28 °C, and the fermentation time is 45 h. Then sterilization is carried out, and centrifugation is carried out at a centrifugal speed of 9000 r / min for 40 min, and the supernatant is taken to obtain the primary product;

[0058] (3) According to 6% of the mass of the primary product, β-cyclodextrin is added to the primary product, and it is stirred at 45 °C at a rotation speed of 450 r / min for 2.8 h; then under 60 °C and -0.06 MPa, vacuum concentration is carried out to a relative density of 1.23, and drying is carried out to obtain the plant ferment; among them, the plant mixture is obtained by mixing selaginella tamariscina, white willow bark and spring elm root in a mass ratio of 1:2.2:3.3; the composite enzyme is obtained by mixing papain and snail enzyme in a mass ratio of 3.4:5; the fermentation bacteria are obtained by mixing lactobacillus plantarum, yeast and acetic acid bacteria in a mass ratio of 3:2:4; the liquid medium is obtained by mixing 3.5 g of melibiose, 2 g of mannan oligosaccharide, 0.5 g of peptone, 1 g of maltose, 0.03 g of urea, 0.02 g of diammonium hydrogen phosphate, 0.01 g of sophorolipid, 30 g of magnesium sulfate and 32 g of water.

[0059] Preparation Example 3

[0060] The plant ferment is prepared by the following method:

[0061] (1) After crushing 100 g of the plant mixture, it is mixed with 2000 g of purified water, and 8 g of a composite enzyme is used for enzymatic treatment. The pH of the enzymatic hydrolysis is 5.5, the temperature is 40 °C, and the time is 2 h; after the enzymatic treatment, sterilization is carried out to obtain the fermentation broth to be fermented;

[0062] (2) 10 g of fermentation bacteria and 45 g of liquid medium are added to the fermentation broth to be fermented, the pH value is adjusted to 5.5, the fermentation temperature is 30 °C, and the fermentation time is 40 h. Then sterilization is carried out, and centrifugation is carried out at a centrifugal speed of 10000 r / min for 30 min, and the supernatant is taken to obtain the primary product;

[0063] (3) Add β-cyclodextrin to the crude product at 8% of the crude product quality, stir at 50 °C at a rotation speed of 500 r / min for 2.5 h; then carry out vacuum concentration at 64 °C and -0.04 MPa until the relative density is 1.25, and dry to obtain the plant ferment; wherein, the plant mixture is obtained by mixing Selaginella tamariscina, white willow bark and Ulmus davidiana var. japonica roots in a mass ratio of 1:2.5:3.5; the composite enzyme is obtained by mixing papain and snail enzyme in a mass ratio of 4:5; the fermenting bacteria are obtained by mixing Lactobacillus plantarum, yeast and Acetobacter in a mass ratio of 4:3:5; the liquid medium is obtained by mixing 4 g of melibiose, 3 g of mannan oligosaccharide, 0.5 g of peptone, 1 g of maltose, 0.03 g of urea, 0.02 g of diammonium hydrogen phosphate, 0.01 g of sophorolipid, 30 g of magnesium sulfate and 35 g of water.

[0064] Preparation Example 4

[0065] The plant ferment is prepared by the following method:

[0066] (1) Crush 100 g of the plant mixture and mix it with 2100 g of purified water, carry out enzymatic treatment with 9 g of the composite enzyme, and the pH of enzymatic hydrolysis is 5.8, the temperature is 45 °C, and the time is 1.5 h; after enzymatic treatment, sterilize to obtain the fermentation broth to be fermented;

[0067] (2) Add 12 g of fermenting bacteria and 55 g of the liquid medium to the fermentation broth to be fermented, adjust the pH value to 6, the fermentation temperature is 32 °C, the fermentation time is 35 h, then sterilize, centrifuge at a centrifugation speed of 11000 r / min for 20 min, take the supernatant to obtain the crude product;

[0068] (3) Add β-cyclodextrin to the crude product at 9% of the crude product quality, stir at 55 °C at a rotation speed of 550 r / min for 2.8 h; then carry out vacuum concentration at 67 °C and -0.02 MPa until the relative density is 1.27, and dry to obtain the plant ferment; wherein, the plant mixture is obtained by mixing Selaginella tamariscina, white willow bark and Ulmus davidiana var. japonica roots in a mass ratio of 1:2.8:3.7; the composite enzyme is obtained by mixing papain and snail enzyme in a mass ratio of 1.1:1; the fermenting bacteria are obtained by mixing Lactobacillus plantarum, yeast and Acetobacter in a mass ratio of 5:4:6; the liquid medium is obtained by mixing 4.5 g of melibiose, 3.5 g of mannan oligosaccharide, 0.5 g of peptone, 1 g of maltose, 0.03 g of urea, 0.02 g of diammonium hydrogen phosphate, 0.01 g of sophorolipid, 30 g of magnesium sulfate and 38 g of water.

[0069] Preparation Example 5

[0070] The plant ferment is prepared by the following method:

[0071] (1) Crush 100 g of the plant mixture and mix it with 2500 g of purified water. Perform enzymatic treatment with 10 g of a composite enzyme, with the pH of enzymatic hydrolysis being 6, the temperature being 50 °C, and the time being 1 h. After enzymatic treatment, sterilize to obtain the fermentation broth to be fermented;

[0072] (2) Add 14 g of fermentation bacteria and 60 g of liquid medium to the fermentation broth to be fermented. Adjust the pH value to 6.5, the fermentation temperature to 35 °C, and the fermentation time to 50 h. Then sterilize, centrifuge at a speed of 12000 r / min for 45 min, and take the supernatant to obtain the primary product;

[0073] (3) Add β-cyclodextrin to the primary product at 10% of the mass of the primary product, and stir at 600 r / min at 60 °C for 3 h. Then, perform vacuum concentration at 70 °C and -0.01 MPa until the relative density is 1.28, and dry to obtain the plant ferment. Among them, the plant mixture is obtained by mixing Selaginella tamariscina, white willow bark, and spring elm root in a mass ratio of 1:3:4; the composite enzyme is obtained by mixing papain and snail enzyme in a mass ratio of 6:5; the fermentation bacteria are obtained by mixing Lactobacillus plantarum, yeast, and Acetobacter aceti in a mass ratio of 6:5:7; the liquid medium is obtained by mixing 5 g of melibiose, 4 g of mannan oligosaccharide, 0.5 g of peptone, 1 g of maltose, 0.03 g of urea, 0.02 g of diammonium hydrogen phosphate, 0.01 g of sophorolipid, 30 g of magnesium sulfate, and 40 g of water.

[0074] Comparative Preparation Example 1

[0075] Comparative Preparation Example 1 is the same as Preparation Example 1, except that: the plant mixture is obtained by mixing Selaginella tamariscina and white willow bark in a mass ratio of 1:2.

[0076] Comparative Preparation Example 2

[0077] Comparative Preparation Example 2 is the same as Preparation Example 1, except that: the plant mixture is obtained by mixing Selaginella tamariscina and spring elm root in a mass ratio of 1:3.

[0078] Comparative Preparation Example 3

[0079] Comparative Preparation Example 3 is the same as Preparation Example 1, except that: the plant mixture is obtained by mixing white willow bark and spring elm root in a mass ratio of 2:3.

[0080] Comparative Preparation Example 4

[0081] Comparative Preparation Example 4 is the same as Preparation Example 1, except that: the plant mixture is only Selaginella tamariscina.

[0082] Comparative Preparation Example 5

[0083] Comparative Preparation Example 5 is the same as Preparation Example 1, except that: the plant mixture is only white willow bark.

[0084] Comparative Preparation Example 6

[0085] Comparative Preparation Example 6 is the same as Preparation Example 1, except that: the plant mixture is only the root of Ulmus propinqua.

[0086] Comparative Preparation Example 7

[0087] Comparative Preparation Example 7 is the same as Preparation Example 1, except that: the complex enzyme is only papain.

[0088] Comparative Preparation Example 8

[0089] Comparative Preparation Example 8 is the same as Preparation Example 1, except that: the complex enzyme is only snail enzyme.

[0090] Comparative Preparation Example 9

[0091] Comparative Preparation Example 9 is the same as Preparation Example 1, except that: the fermenting bacteria are obtained by mixing Lactobacillus plantarum and Acetobacter aceti in a mass ratio of 2:3.

[0092] Comparative Preparation Example 10

[0093] Comparative Preparation Example 10 is the same as Preparation Example 1, except that: the fermenting bacteria are obtained by mixing Saccharomyces cerevisiae and Acetobacter aceti in a mass ratio of 1:3.

[0094] Comparative Preparation Example 11

[0095] Comparative Preparation Example 11 is the same as Preparation Example 1, except that: the fermenting bacteria are obtained by mixing Lactobacillus plantarum and Saccharomyces cerevisiae in a mass ratio of 2:1.

[0096] Comparative Preparation Example 12

[0097] Comparative Preparation Example 12 is the same as Preparation Example 1, except that: the fermenting bacteria are only Lactobacillus plantarum.

[0098] Comparative Preparation Example 13

[0099] Comparative Preparation Example 13 is the same as Preparation Example 1, except that: the fermenting bacteria are only Acetobacter aceti.

[0100] Comparative Preparation Example 14

[0101] Comparative Preparation Example 14 is the same as Preparation Example 1, except that: the fermenting bacteria are only Saccharomyces cerevisiae.

[0102] Comparative Preparation Example 15

[0103] Comparative Preparation Example 15 is the same as Preparation Example 1, except that: in step (3), β-cyclodextrin is not added, and the crude product is directly subjected to vacuum concentration and drying to obtain the plant ferment.

[0104] Comparative Preparation Example 16

[0105] Comparative Preparation Example 16 is the same as Preparation Example 1, except that in step (1), 100 g of the plant mixture is pulverized and then mixed with 1500 g of purified water to obtain the liquid to be fermented.

[0106] Examples 1-5 provide a skin barrier repair cream and a preparation method thereof.

[0107] Example 1

[0108] A skin barrier repair cream, comprising the following raw materials: 11 g of plant ferment, 1 g of water lily extract, 0.8 g of buddleja officinalis extract, 1.2 g of paeonia lactiflora extract, 0.6 g of cetearyl olivate, 0.1 g of sorbitan olivate, 0.6 g of hydrolyzed sclerotium gum, 1 g of carbomer, 1 g of betaine, 2 g of hyaluronic acid, 0.5 g of glycerin, 15 g of water, 0.1 g of fumed silica, 0.2 g of montmorillonite;

[0109] Among them, the plant ferment is prepared from Preparation Example 1;

[0110] A skin barrier repair cream comprises the following steps:

[0111] S1. Fumed silica and montmorillonite are sufficiently ground to 200 mesh, then plasma treatment is carried out. After vacuum pumping, nitrogen is introduced, and the gas flow rate is 20 cm 3 / s, at a power of 80 KW, a voltage of 380 V, and a treatment time of 200 s to obtain a powder;

[0112] S2. Cetearyl olivate, sorbitan olivate, and tocopheryl acetate are heated to 80 °C and stirred and dissolved at a rotation speed of 100 r / min to obtain mixture A, which is kept warm for standby;

[0113] S3. Water, glycerin, hydrolyzed sclerotium gum, carbomer, betaine, and hyaluronic acid are heated to 85 °C and dissolved completely to obtain mixture B, which is kept warm for standby;

[0114] S4. Mixture A is slowly added to mixture B. After homogenizing and emulsifying at 40 MPa and a rotation speed of 2000 rpm for 5 min, the temperature is lowered to 60 °C, the powder is added, and the same homogenizing rotation speed and pressure are maintained for 3 min. The temperature is further lowered to 40 °C, and the plant ferment, water lily extract, buddleja officinalis extract, and paeonia lactiflora extract are added and stirred until uniform, and then steam sterilized for 5 min to obtain the skin barrier repair cream.

[0115] Example 2

[0116] A skin barrier repair cream, comprising the following raw materials: 12 g of plant ferment, 1.5 g of water lily extract, 1 g of buddleja officinalis extract, 1.5 g of peony flower extract, 0.8 g of cetearyl olivate, 0.4 g of sorbitan olivate, 0.8 g of hydrolyzed sclerotium gum, 1.2 g of carbomer, 1.2 g of betaine, 2.4 g of hyaluronic acid, 0.7 g of glycerin, 18 g of water, 0.11 g of fumed silica, 0.23 g of montmorillonite;

[0117] Among them, the plant ferment is prepared from Preparation Example 2;

[0118] A skin barrier repair cream, comprising the following steps:

[0119] S1. After the fumed silica and montmorillonite are sufficiently ground to 250 meshes, plasma treatment is carried out. After vacuum pumping, nitrogen is introduced, and the gas flow rate is 25 cm 3 / s, at a power of 80 KW, a voltage of 380 V, and a treatment time of 220 s, to obtain a powder;

[0120] S2. Heat the cetearyl olivate, sorbitan olivate, and tocopheryl acetate to 82 °C, stir and dissolve at a rotation speed of 120 r / min to obtain a mixture A, and keep it warm for standby;

[0121] S3. Heat the water, glycerin, hydrolyzed sclerotium gum, carbomer, betaine, and hyaluronic acid to 86 °C and dissolve completely to obtain a mixture B, and keep it warm for standby;

[0122] S4. Slowly add the mixture A to the mixture B, homogenize and emulsify at 60 MPa and a rotation speed of 2200 rpm for 6 min, then cool down to 62 °C, add the powder, keep the same homogenization rotation speed and pressure, homogenize for 3.5 min, continue to cool down to 42 °C, add the plant ferment, water lily extract, buddleja officinalis extract, and peony flower extract, stir until uniform, and sterilize with steam for 6 min to obtain the skin barrier repair cream.

[0123] Example 3

[0124] A skin barrier repair cream, comprising the following raw materials: 13 g of plant ferment, 2 g of water lily extract, 1.2 g of buddleja officinalis extract, 2 g of peony flower extract, 0.9 g of cetearyl olivate, 0.3 g of sorbitan olivate, 0.8 g of hydrolyzed sclerotium gum, 1.5 g of carbomer, 1.5 g of betaine, 3 g of hyaluronic acid, 0.8 g of glycerin, 23 g of water, 0.13 g of fumed silica, 0.25 g of montmorillonite;

[0125] Among them, the plant ferment is prepared from Preparation Example 3;

[0126] A skin barrier repair cream, comprising the following steps:

[0127] S1. After thoroughly grinding fumed silica and montmorillonite to 250 mesh, plasma treatment is carried out. After evacuating, nitrogen is introduced, and the gas flow rate is 30 cm 3 / s. At a power of 80 KW, a voltage of 380 V, and a treatment time of 250 s, powder is obtained;

[0128] S2. Heat cetearyl olivate, sorbitan olivate, and tocopheryl acetate to 84 °C and stir to dissolve at a rotation speed of 180 r / min to obtain mixture A, and keep it warm for standby;

[0129] S3. Heat water, glycerin, hydrolyzed sclerotium gum, carbomer, betaine, and hyaluronic acid to 88 °C and dissolve completely to obtain mixture B, and keep it warm for standby;

[0130] S4. Slowly add mixture A to mixture B. After homogenizing and emulsifying at 70 MPa and a rotation speed of 2500 rpm for 8 min, cool down to 65 °C, add the powder, maintain the same homogenization rotation speed and pressure, homogenize for 4 min, continue to cool down to 45 °C, add the plant ferment, water lily extract, buddleja officinalis extract, and paeonia lactiflora extract and stir until uniform, and sterilize with steam for 7 min to obtain the skin barrier repair cream.

[0131] Example 4

[0132] A skin barrier repair cream, including the following raw materials: 14 g of plant ferment, 2.5 g of water lily extract, 1.5 g of buddleja officinalis extract, 2.2 g of paeonia lactiflora extract, 1 g of cetearyl olivate, 0.4 g of sorbitan olivate, 1.1 g of hydrolyzed sclerotium gum, 1.8 g of carbomer, 1.9 g of betaine, 2.9 g of hyaluronic acid, 0.8 g of glycerin, 28 g of water, 0.14 g of fumed silica, 0.28 g of montmorillonite;

[0133] Among them, the plant ferment is prepared from Preparation Example 4;

[0134] A skin barrier repair cream, including the following steps:

[0135] S1. After thoroughly grinding fumed silica and montmorillonite to 250 mesh, plasma treatment is carried out. After evacuating, nitrogen is introduced, and the gas flow rate is 35 cm 3 / s. At a power of 80 KW, a voltage of 380 V, and a treatment time of 280 s, powder is obtained;

[0136] S2. Heat cetearyl olivate, sorbitan olivate, and tocopheryl acetate to 85 °C and stir to dissolve at a rotation speed of 180 r / min to obtain mixture A, and keep it warm for standby;

[0137] S3, heating water, glycerin, hydrolyzed sclerotium gum, carbomer, betaine, and hyaluronic acid to 89° C. to dissolve completely to obtain a mixture B, which is kept warm for later use;

[0138] S4. Slowly add mixture A to mixture B, homogenize and emulsify at 90 MPa and 2800 rpm for 9 minutes, then cool to 68°C, add powder, maintain the same homogenization speed and pressure, homogenize for 4.5 minutes, continue to cool to 48°C, add plant fermentation, water lily extract, buddleja monnieri extract, and peony flower extract, stir until uniform, and sterilize with steam for 7 minutes to obtain a skin barrier repair cream.

[0139] Example 5

[0140] A skin barrier repairing cream, comprising the following raw materials: 15g of plant fermentation, 3g of water lily extract, 1.6g of buddleja extract, 2.4g of peony flower extract, 1.1g of cetearyl olivate, 0.5g of sorbitan olivate, 1.2g of hydrolyzed sclerotium gum, 2g of carbomer, 2g of betaine, 4g of hyaluronic acid, 0.9g of glycerin, 30g of water, 0.15g of fumed silica, and 0.3g of montmorillonite;

[0141] Wherein, the plant fermentation product is prepared by Preparation Example 5;

[0142] A skin barrier repairing cream comprises the following steps:

[0143] S1, fumed silica and montmorillonite were fully ground to 300 mesh, and then plasma treated. After vacuuming, nitrogen was introduced with a gas flow rate of 40 cm 3 / s, at a power of 80KW, a voltage of 380V, and a processing time of 300s, powder was obtained;

[0144] S2, heating cetearyl olivate, sorbitan olivate, and tocopherol acetate to 85° C., stirring and dissolving at a speed of 100-200 r / min to obtain a mixture A, and keeping the mixture warm for later use;

[0145] S3, heating water, glycerin, hydrolyzed sclerotium gum, carbomer, betaine, and hyaluronic acid to 890° C. to dissolve completely to obtain a mixture B, which is kept warm for later use;

[0146] S4. Slowly add mixture A to mixture B, homogenize and emulsify at 100 MPa and 3000 rpm for 10 min, then cool to 70°C, add powder, maintain the same homogenization speed and pressure, homogenize for 5 min, continue to cool to 50°C, add plant fermentation, water lily extract, buddleja extract, and peony flower extract, stir until uniform, and sterilize with steam for 8 min to obtain a skin barrier repair cream.

[0147] To verify the properties of a skin barrier repair cream provided by this application, the applicant set Comparative Examples 1-19, where:

[0148] Comparative Example 1

[0149] Comparative Example 1 is the same as Example 1, except that: the plant ferment is prepared from Comparative Preparation Example 1.

[0150] Comparative Example 2

[0151] Comparative Example 2 is the same as Example 1, except that: the plant ferment is prepared from Comparative Preparation Example 2.

[0152] Comparative Example 3

[0153] Comparative Example 3 is the same as Example 1, except that: the plant ferment is prepared from Comparative Preparation Example 3.

[0154] Comparative Example 4

[0155] Comparative Example 4 is the same as Example 1, except that: the plant ferment is prepared from Comparative Preparation Example 4.

[0156] Comparative Example 5

[0157] Comparative Example 5 is the same as Example 1, except that: the plant ferment is prepared from Comparative Preparation Example 5.

[0158] Comparative Example 6

[0159] Comparative Example 6 is the same as Example 1, except that: the plant ferment is prepared from Comparative Preparation Example 6.

[0160] Comparative Example 7

[0161] Comparative Example 7 is the same as Example 1, except that: the plant ferment is prepared from Comparative Preparation Example 7.

[0162] Comparative Example 8

[0163] Comparative Example 8 is the same as Example 1, except that: the plant ferment is prepared from Comparative Preparation Example 8.

[0164] Comparative Example 9

[0165] Comparative Example 9 is the same as Example 1, except that: the plant ferment is prepared from Comparative Preparation Example 9.

[0166] Comparative Example 10

[0167] Comparative Example 10 is the same as Example 1, except that: the plant ferment is prepared from Comparative Preparation Example 10.

[0168] Comparative Example 11

[0169] Comparative Example 11 was the same as Example 1, except that the plant ferment was prepared from Comparative Preparation Example 11.

[0170] Comparative Example 12

[0171] Comparative Example 12 was the same as Example 1, except that the plant ferment was prepared from Comparative Preparation Example 12.

[0172] Comparative Example 13

[0173] Comparative Example 13 was the same as Example 1, except that the plant ferment was prepared from Comparative Preparation Example 13.

[0174] Comparative Example 14

[0175] Comparative Example 14 was the same as Example 1, except that the plant ferment was prepared from Comparative Preparation Example 14.

[0176] Comparative Example 15

[0177] Comparative Example 15 was the same as Example 1, except that the plant ferment was prepared from Comparative Preparation Example 15.

[0178] Comparative Example 16

[0179] Comparative Example 16 was the same as Example 1, except that the plant ferment was prepared from Comparative Preparation Example 16.

[0180] Comparative Example 17

[0181] Comparative Example 17 was the same as Example 1, except that an equal amount of fumed silica was used to replace montmorillonite.

[0182] Comparative Example 18

[0183] Comparative Example 18 was the same as Example 1, except that an equal amount of montmorillonite was used to replace fumed silica.

[0184] Comparative Example 19

[0185] Comparative Example 19 was the same as Example 1, except that the operation in step S1 was as follows: fumed silica and montmorillonite were sufficiently ground to 200 mesh, and then powder was obtained.

[0186] In order to detect the properties of the skin barrier repair cream prepared in Examples 1-5 and Comparative Examples 1-19 of the present application, the following experiments were carried out:

[0187] (1) Antioxidant performance test

[0188] Add 2.0 mL of DPPH test solution with a mass concentration of 0.04 mg / mL and the sample to be tested into a test tube. Make up the total volume to 3 mL with absolute ethanol. Shake well. After reacting in the dark for 30 min, measure the absorbance at a wavelength of 517 nm and record it as A 10 Add 2 mL of absolute ethanol and the corresponding volume of the sample to be tested into a test tube. Make up the total volume to 3 mL with absolute ethanol. Record the measured absorbance as A 11 Add 2 mL of DPPH test solution and 1 mL of absolute ethanol into a test tube. Record the measured absorbance as A 12 The calculation formula for the DPPH free radical scavenging rate is: Y' = [1 - (A 10 - A 11 ) / A 12 × 100%; where A 10 is the absorbance value of the system after the repair skin barrier cream provided in Examples 1 - 5 and Comparative Examples 1 - 19 scavenges DPPH, A 11 is the absorbance value of the system after the blank sample control scavenges DPPH, and A 12 is the absorbance value of the reaction system before adding the repair skin barrier cream. The test results are shown in Table 1;

[0189] (2) Test for the performance of promoting collagen synthesis

[0190] a. Influence on the proliferation of fibroblasts

[0191] Inoculate fibroblasts in the logarithmic growth phase into a 96-well plate at a density of 1×10 5 cells / mL. Divide the cells in the 96-well plate into 25 groups. Among them, 25 groups correspond to the repair skin barrier creams provided in Examples 1 - 5 and Comparative Examples 1 - 19 respectively, and the remaining 1 group is the blank control group; Add the sample to be tested into the cell wells respectively, so that the concentration of the repair skin barrier cream in the wells is 5 μg / mL, and add an equal volume of DMEM medium to the blank control group; Incubate the fibroblasts treated in the above 25 groups under the conditions of 5% CO2, 37 °C constant temperature and humidity for 72 h. Use a cell counter to count the 25 groups of cells, and count the number of cells respectively to obtain the influence results of the repair skin barrier cream described in this application on the proliferation of fibroblasts. The specific data are shown in Table 1.

[0192] b. Influence on the collagen expression of fibroblasts

[0193] Detection of the mRNA level of type I collagen (collagen I): Inoculate fibroblasts in the logarithmic growth phase into a 96-well plate at a density of 1×10 5The cells in the 96-well plate were inoculated at a density of 100 μg / mL in a 96-well plate, and the cells in the 96-well plate were divided into 25 groups, 25 of which corresponded to the repairing skin barrier cream provided in Examples 1-5 and Comparative Examples 1-19, respectively, and the remaining 1 group was a blank control group; the samples to be tested were added to the cell wells respectively so that the concentration of the repairing skin barrier cream in the wells was 5 μg / mL, and an equal volume of DMEM culture medium was added to the blank control group; the fibroblasts treated by the above 25 groups were incubated under 5% CO2, 37°C constant temperature and humidity conditions for 72 h, and collected; total RNA was extracted using RNAiso Plus reagent (9109Takara), and then the mRNA was reverse transcribed into cDNA using PrimeScriptRT kit, and then an equal amount of cDNA was taken to perform real-time fluorescence PCR to detect the mRNA level of collagen I using Real-Time PCR Master Mix kit (ABI) and primers, and the effect of the skin barrier cream described in the present application on the mRNA level of collagen I in fibroblasts was obtained, and the specific data are shown in Table 1.

[0194] The results are shown in Table 1.

[0195] Table 1:

[0196]

[0197]

[0198] It can be seen from the data shown in Table 1 above that: compared with the blank control group, the DPPH free radical scavenging rate of the skin barrier repairing cream provided by Examples 1-5 and Comparative Examples 1-19 is higher, which can promote collagen synthesis, and the test results of the skin barrier repairing cream obtained in Examples 1-5 are better than those in Comparative Examples 1-19; it fully demonstrates that the cream of the present application has a significant skin repairing effect.

[0199] It can be seen from Example 1 and Comparative Examples 1-6 that the plant fermentation product in Example 1 is prepared by Preparation Example 1, and the plant mixture in Preparation Example 1 is obtained by mixing Selaginella, white willow bark and elm root. Compared with Comparative Examples 1-6, it can be seen that the skin barrier repair cream obtained in Example 1 has better performance, indicating that Selaginella, white willow bark and elm root have synergistic effects, so that the plant fermentation product contains rich effective ingredients.

[0200] It can be seen from Example 1 and Comparative Examples 7 and 8 that the plant fermentation product in Example 1 is prepared by Preparation Example 1, and the composite enzyme in Preparation Example 1 is obtained by mixing papain and snail enzyme. Compared with Comparative Examples 7 and 8, the skin barrier repairing cream obtained in Example 1 has better antioxidant properties and collagen promotion properties.

[0201] As can be seen from Example 1 and Comparative Examples 9-14: The plant ferment in Example 1 was prepared from Preparation Example 1. The fermenting bacteria in Preparation Example 1 were obtained by mixing Lactobacillus plantarum, yeast, and Acetobacter. Compared with Comparative Examples 9-14, the antioxidant effect, cell proliferation promotion effect, and collagen production promotion effect of the skin barrier repair cream obtained in Example 1 were significantly better than those in Comparative Examples 9-14.

[0202] As can be seen from Example 1 and Comparative Example 15: The plant ferment in Example 1 was prepared from Preparation Example 1. β-Cyclodextrin was added in step (3) of Preparation Example 1. Compared with Comparative Example 15, the overall performance of the skin barrier repair cream obtained in Example 1 was greatly improved.

[0203] As can be seen from Example 1 and Comparative Example 16: The plant ferment in Example 1 was prepared from Preparation Example 1. A complex enzyme was added for enzymatic hydrolysis in step (1) of Preparation Example 1. Compared with Comparative Example 16, the cream obtained in Example 1 had a significant effect on skin repair, indicating that enzymatic hydrolysis of the plant ferment followed by fermentation helped to obtain more active ingredients.

[0204] As can be seen from Example 1 and Comparative Examples 17 and 18: The raw materials in Example 1 contained fumed silica and montmorillonite. Compared with Comparative Examples 17 and 18, the cream obtained in Example 1 had excellent antioxidant and skin repair functions, indicating that fumed silica and montmorillonite could synergistically improve the comprehensive performance of the cream.

[0205] As can be seen from Example 1 and Comparative Example 19: The fumed silica and montmorillonite in Example 1 were treated by plasma. Compared with Comparative Example 19, the cream obtained in Example 1 had outstanding skin repair effects and broad application prospects.

[0206] This specific embodiment is only an interpretation of the present application and does not limit the present application. After reading this specification, those skilled in the art can make modifications to this embodiment without creative contributions as needed, but as long as it is within the scope of the claims of the present application, it is protected by the patent law.

Claims

1. A skin barrier repair cream, characterized in that, The invention comprises the following raw materials in parts by weight: 11-15 parts of plant fermentation, 1-3 parts of water lily extract, 0.8-1.6 parts of buddleja flower extract, 1.2-2.4 parts of peony flower extract, 0.6-1.1 parts of cetearyl olivate, 0.1-0.5 parts of sorbitan olivate, 0.2-0.8 parts of tocopherol acetate, 0.6-1.2 parts of hydrolyzed sclerotium gum, 1-2 parts of carbomer, 1-2 parts of betaine, 2-4 parts of hyaluronic acid, 0.5-0.9 parts of glycerin, 15-30 parts of water, 0.1-0.15 parts of fumed silica and 0.2-0.3 parts of montmorillonite; The plant fermented product is obtained by mixing the plant mixture with the fermentation bacteria and then fermenting; The plant mixture is obtained by mixing Selaginella, white willow bark and elm root in a mass ratio of 1:2-3:3-4, and the fermentation bacteria are obtained by mixing Lactobacillus plantarum, yeast and acetobacter in a mass ratio of 2-6:1-5:3-7; The plant fermentation product is prepared by the following method: (1) crushing the plant mixture and mixing it with purified water, treating it with a composite enzyme, sterilizing it after the enzyme treatment, and obtaining a fermentation liquid, wherein the composite enzyme is obtained by mixing papain and snail enzyme in a mass ratio of 3-6:5; (2) Mixing the fermentation liquid, fermentation bacteria, and liquid culture medium, fermenting, sterilizing, centrifuging, and taking the supernatant to obtain the primary product; (3) adding β-cyclodextrin to the primary product, heating and stirring for a period of time, and then reducing the pressure to concentrate and dry to obtain a plant fermentation product; The preparation method of the skin barrier repairing cream comprises the following steps: S1, fumed silica and montmorillonite are fully ground and then subjected to plasma treatment to obtain powder; S2, heating cetearyl olivate, sorbitan olivate, and tocopherol acetate to 80-85° C., stirring to dissolve, to obtain a mixture A, and keeping the mixture warm for later use; S3, heating water, glycerin, hydrolyzed sclerotium gum, carbomer, betaine, and hyaluronic acid to 85-90° C. to dissolve completely to obtain a mixture B, which is kept warm for later use; S4. Slowly add mixture A to mixture B, homogenize and emulsify for 5-10 minutes, cool to 60-70°C, add powder, homogenize for 3-5 minutes, continue to cool to 40-50°C, add plant fermentation, water lily extract, buddleja extract, and peony flower extract, stir until uniform, and sterilize with steam for 5-8 minutes to obtain a skin barrier repair cream.

2. The skin barrier repair cream according to claim 1, wherein The mass ratio of the plant mixture, purified water and complex enzyme is 1:15-25:0.06-0.

1.

3. The skin barrier repair cream according to claim 1, wherein The mass ratio of the plant mixture, the fermentation bacteria and the liquid culture medium is 100:7-14:30-60.

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