An apparatus and method for uniform crystallization of a solution on a surface of a carrier under examination

Abstract

The present application is a new type of medical equipment and detection technology to solve the problem that the non-homogeneous test solution, especially the blood containing a large amount of protein, cannot be uniformly crystallized on the test carrier, specifically a device and method for uniformly crystallizing the solution on the surface of the test carrier. Mainly includes: vacuum temperature control box system, vacuum system and drop system, and through the coordination of the vacuum temperature control box, vacuum assembly and solution pretreatment machine, the solution temperature, the working temperature in the cavity and the vacuum degree are accurately controlled, so that the solution covered on the surface of the carrier is uniformly distributed and the thickness is the same after crystallization, thereby improving the consistency and stability of the subsequent carrier surface sample detection process, reducing the test error and volatility.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of medical equipment and detection technology, in particular to a device and method for uniform crystallization of a solution on a detection carrier surface. BACKGROUND

[0002] Currently, in the solution crystallization detection technology, the sample to be detected is usually prepared in the form of a solution, and then dropped on the upper layer of the detection carrier for crystallization. However, since the sample to be detected is often a heterogeneous solution, especially a solution such as blood and various macromolecular proteins, and the micro surface of the detection chip carrier is also not flat, it is difficult for the solution to form a uniform layer of solution crystals on the detection carrier surface. The uniformity of the crystals determines the accuracy of the sample detection, so how to obtain uniform solution crystals on the carrier to improve the detection accuracy and success rate has become a major problem in the industry. SUMMARY

[0003] In order to solve the problem that the solution to be detected, especially a non-homogeneous solution such as blood containing a large amount of protein, cannot be uniformly crystallized on the detection carrier, the present application designs a device and method for uniform crystallization of a solution on a detection carrier surface, and the specific implementation includes:

[0004] A device for uniform crystallization of a solution on a detection carrier surface, comprising a vacuum temperature control box system, a vacuum system, and a liquid drop system, the detection carrier is located in the vacuum temperature control box system, the vacuum system is connected with the vacuum temperature control box system to control the vacuum degree of the vacuum temperature control box system, and the liquid drop system is used to drop the solution to be detected on the detection carrier.

[0005] According to another specific embodiment of the present application, the device further comprises a solution pretreatment machine (15).

[0006] According to another specific embodiment of the present application, the vacuum temperature control box system comprises a vacuum temperature control box cover (12) and a vacuum temperature control box (13), the vacuum temperature control box (13) is a box structure and comprises a temperature control device; the vacuum temperature control box cover (12) is used to control the sealing and opening and closing of the vacuum temperature control box system; the vacuum system comprises a vacuum air duct and a vacuum component (14), one end of the vacuum air duct is connected with the vacuum temperature control box system, and the other end is connected with the vacuum component (14); and the liquid drop system comprises a pipettor (6) and a moving mechanism.

[0007] According to another specific embodiment of the present application, the temperature control range of the vacuum temperature control box system is between -5 and 40 degrees Celsius, the vacuum degree of the vacuum system is between -0.6 and -1.0 bar, and the temperature control range of the solution pretreatment machine (15) is between -5 and 40 degrees Celsius.

[0008] According to another specific embodiment of the present application, the moving mechanism is an XYZ three-direction slide assembly or a mechanical arm or a manual support.

[0009] The specific embodiment of the present application also discloses a method for making a solution uniformly crystallize on a surface of a carrier to be detected, comprising:

[0010] S1: start a vacuum temperature control box system and adjust the temperature;

[0011] S2: when the temperature reaches the process requirement, open the vacuum temperature control box cover (12);

[0012] S3: place the carrier (19) into the vacuum temperature control box system;

[0013] S4: after the drop system absorbs the solution to be detected, drop the solution to be detected on the surface of the carrier (19) according to the detection process requirement;

[0014] S5: close the vacuum temperature control box cover (12);

[0015] S6: then start a vacuum system, vacuumize the space in the vacuum temperature control box system, and adjust the vacuum degree according to the process;

[0016] S7: wait for the solution to be detected to crystallize;

[0017] S8: when the solution to be detected is crystallized on the carrier, close the vacuum assembly (14);

[0018] S9: take out the crystallized carrier (19), place a new carrier rack (18) and carrier (19).

[0019] According to another specific embodiment of the present application, the temperature of the vacuum temperature control box system is between -5 and 40 degrees Celsius, and the vacuum degree is between -0.6 and -1.0 bar.

[0020] According to another specific embodiment of the present application, in the step of starting the vacuum temperature control box system and adjusting the temperature, the temperature is set in the range of -5 to 25 degrees Celsius for a macromolecular protein; and in the step of then starting the vacuum system, vacuumizing the space in the vacuum temperature control box system, and adjusting the vacuum degree according to the process, the vacuum degree is set to be -0.6 to -0.8 bar.

[0021] According to another specific embodiment of the present application, for small molecule protein, the solution needs to be pretreated, the temperature of the solution to be detected is controlled between 10-35 degrees Celsius, the processing time is about 5-10 minutes, the temperature setting in the step of starting the vacuum temperature control box system and adjusting the temperature is set in the range of 0 to 35 degrees Celsius; the vacuum degree setting in the step of starting the vacuum system and vacuumizing the space in the vacuum temperature control box system and adjusting the vacuum degree according to the process is set in the range of -0.7 to -1.0 bar.

[0022] According to another specific embodiment of the present application, the temperature in the step of starting the vacuum temperature control box system and adjusting the temperature is higher than the temperature after the solution to be detected is pretreated.

[0023] According to another specific embodiment of the present application, in the step of starting the vacuum system and vacuumizing the space in the vacuum temperature control box system and adjusting the vacuum degree according to the process, air is added according to the properties of the solution to be detected, so that the vacuum degree of the space in the vacuum temperature control box system presents a wavy curve.

[0024] The device and method for making the solution uniformly crystallize on the surface of the carrier according to the present application can precisely control the solution temperature, the working temperature in the cavity and the vacuum degree by the coordination of the vacuum temperature control box, the vacuum assembly and the solution pretreatment machine, so that the solution covering the surface of the carrier is uniformly distributed and has the same thickness after crystallization, thereby improving the consistency and stability of the subsequent carrier surface sample detection process, and reducing the test error and volatility. BRIEF DESCRIPTION OF DRAWINGS

[0025] The present application will be further described below in conjunction with the drawings and specific embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application, and are not used to limit the present application.

[0026] Figure 1 It is a three-dimensional schematic view of the overall structure of the present application;

[0027] Figure 2 It is a three-view of the present application;

[0028] Figure 3 It is a schematic view of the carrier and carrier frame used in the present application;

[0029] Figure 4 It is a pressure curve schematic view of the vacuum temperature control box in the vacuumizing process of the present application;

[0030] The reference signs are: 1, operation workbench; 2, bracket column; 3, Y-axis sliding table assembly 4, X-axis sliding table assembly; 5, Z-axis sliding table assembly; 6, pipette; 7, vacuum temperature-controlled box cover moving sliding table assembly; 8, cylinder connecting plate; 9, cover opening cylinder; 10, guide column; 11, waste suction head box; 12, vacuum temperature-controlled box cover; 13, vacuum temperature-controlled box; 14, vacuum assembly; 15, solution pretreatment machine; 16, suction head box; 17, suction head; 18, carrier rack; 19, carrier. DETAILED DESCRIPTION

[0031] The present application is described in detail below by specific embodiments, and other advantages and effects of the present application can be easily understood by those skilled in the art from the disclosure of the specification. Although the description of the present application will be introduced in combination with the preferred embodiments, this does not mean that the features of the present application are limited to this embodiment. On the contrary, the purpose of introducing the application in combination with the embodiments is to cover other options or modifications that can be extended based on the claims of the present application. In order to provide a deep understanding of the present application, many specific details will be included in the following description. The present application can also be implemented without using these details. In addition, in order to avoid confusion or obscure the focus of the present application, some specific details will be omitted in the description. It should be noted that the embodiments in the present application and the features in the embodiments can be combined with each other without conflict.

[0032] It should be noted that in the present specification, similar reference signs and letters represent similar items in the following drawings, so once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings.

[0033] In the description of the present embodiment, it should be noted that the terms "upper", "lower", "inner", "bottom" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship commonly placed when the product of the present application is used, and are only for the convenience of describing the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.

[0034] The terms "first", "second", and the like are only used to distinguish the description, and cannot be understood as indicating or implying relative importance.

[0035] In the description of the embodiments, it should also be noted that unless specifically defined and limited, the terms "set", "connected", "connected" should be understood broadly, for example, it can be fixedly connected, or detachably connected, or integrally connected, it can be mechanically connected, or electrically connected, it can be directly connected, or indirectly connected through an intermediate medium, it can be the communication inside two elements. For those skilled in the art, the specific meaning of the above terms in the embodiments can be understood according to the specific circumstances.

[0036] In order to make the purpose, technical scheme and advantages of the present application clearer, the embodiments of the present application will be further described in detail below with reference to the drawings.

[0037] In view of the problems of inaccurate sample solution drop position on the carrier and uneven crystallization distribution during manual operation, the purpose of the present application is to provide a device and method for uniform crystallization of solution on the surface of a detected carrier (the detected carrier is mainly a detection chip with a silicon-based substrate surface attached with micro lines or undulations, and the surface can be coated with a plating film, which mainly detects the components in the crystalline substance through current detection or light diffraction detection method). The drop position, working temperature and working vacuum degree can be accurately adjusted to realize uniform distribution of the crystalline body and improve the stability of detection.

[0038] In order to achieve the above purpose, the present application builds a specific device system, which includes a vacuum temperature control box system, a vacuum system and a drop system.

[0039] The vacuum temperature control box system is a device that can provide a relatively stable temperature environment for the detected carrier, which can ensure that the environmental temperature of the carrier and the detected solution is controlled in a reasonable and stable range. The vacuum temperature control box system mainly includes a vacuum temperature control box cover 12 and a vacuum temperature control box 13. The vacuum temperature control box 13 is a box structure with heat preservation function, and is equipped with a refrigeration or heating device for adjusting the temperature in the box, which can control the temperature in the box. The vacuum temperature control box cover 12 is used to control the sealing and opening of the vacuum temperature control box system, so as to facilitate the entry and exit of the detected carrier and the detected solution.

[0040] The vacuum system is connected with the vacuum temperature control box system, and the vacuum system is used to control the vacuum degree in the vacuum temperature control box system, which is a device that can provide a negative pressure environment for the detected carrier and the detected solution, and can ensure that the detected solution has a stable negative pressure environment when it is solidified on the detected carrier. The vacuum system mainly includes a vacuum air duct and a vacuum component 14. One end of the vacuum air duct is connected with the vacuum temperature control box system, and the other end is connected with the vacuum component 14.

[0041] In some possible embodiments, a gas capturing device is arranged at the gas outlet end of the vacuum component, which is used to detect the substances in the evaporated gas.

[0042] The droplet system is used to drop the solution to be detected on the carrier. The droplet system comprises a pipette 6, which can be installed on a set of slide table assembly or mechanical arm moving mechanism, in order to ensure the position accuracy and stability of the droplets. The pipette 6 can adopt a high-precision droplet or a microfluidic chip.

[0043] In some possible embodiments, the device for uniform crystallization of a solution on the surface of a carrier according to the present application is further provided with a set of pretreatment equipment, which comprises a solution pretreatment machine 15. The solution pretreatment machine 15 comprises heating and cooling equipment, and can adjust the temperature of the solution to be detected. The solution to be detected is placed in the solution pretreatment machine 15.

[0044] The carrier to be detected is placed in a vacuum temperature control box system during use. An interface is arranged on the vacuum temperature control box system and connected with a vacuum system. After the solution to be detected is dropped on the carrier by the droplet system, the vacuum temperature control box cover 12 is closed and the temperature is reduced to a set temperature. During detection, specific temperature parameters can be selected according to the characteristics of different solutions. Generally, when the vacuum temperature control box 13 can meet the temperature range of-5-40℃, most solution types can be met.

[0045] When the vacuum temperature control box system reaches the set temperature value, the vacuum system starts to draw vacuum, and the vacuum degree in the vacuum temperature control box is drawn to a set value (the same solution under different temperature variation rates and vacuum rates will affect the crystallization results, including size, shape and uniformity. Generally, the vacuum degree is between-0.6 and-1.0 bar). Under this environment, the solution to be detected on the carrier naturally solidifies to form a uniform film, which can be used for detection.

[0046] In some possible embodiments, air is added to the vacuum temperature control box 13 during the vacuum drawing process according to the properties of the solution to be detected, so that the air pressure in the vacuum temperature control box 13 rises, and the vacuum degree in the vacuum temperature control box 13 presents a wavy curve during the vacuum drawing process, which can effectively improve the success rate of uniform crystallization.

[0047] In order to ensure the influence of the droplet amount on the solidification effect, the error accuracy of the droplet system during droplet can reach ±0.5 μl. In addition, in order to improve the use, a tip box 16 for placing a disposable tip 17 and a carrier rack 18 for placing a carrier 19 can also be arranged.

[0048] Example one: one possible embodiment of the device for uniform crystallization of a solution on the surface of a carrier according to the present application is as follows Figure 1 、 2As shown, it includes: operation workbench 1, droplet system, vacuum temperature control box system, vacuum system, the droplet system includes XYZ three direction slide table assembly, pipette 6; the vacuum temperature control box system includes vacuum temperature control box cover moving slide table assembly 7, cylinder connecting plate 8, uncovering cylinder 9, guide column 10, vacuum temperature control box cover 12, vacuum temperature control box 13, carrier frame 18; the vacuum system includes vacuum airway and vacuum assembly 14.

[0049] Operation workbench 1 is used to place various parts in the device described in the application, and the XYZ three direction slide table assembly is arranged on the workbench 1, which includes Y-axis slide table assembly 3, X-axis slide table assembly 4 and Z-axis slide table assembly 5, respectively used to control the pipette 6 to slide in X, Y and Z directions.

[0050] The pipette 6 adopts a high-precision droplet dispenser. In this embodiment, a "Rening" droplet dispenser is used as an example. According to different detection quantities, the corresponding model is selected, and the precision is ±0.5 μl. It should be noted that the aforementioned XYZ three direction slide table assembly structure is mainly used to accurately control the spatial position of the pipette 6, so that it can accurately and freely move between the required working position (i.e. the center position of the carrier to be detected or other positions where the detected solution needs to be dropped) and the standby position or other positions. To achieve this function, the aforementioned XYZ three direction slide table assembly structure can also use a mechanical arm, manual control and other forms to achieve the function, as long as it can meet the aforementioned functional requirements. It should belong to a part of the droplet system of the application, and belong to the disclosure range and protection range of the application.

[0051] The vacuum temperature control box 13 is a box structure, which contains a cooling device for temperature control. The carrier frame 18 is located in the vacuum temperature control box 13, and the carrier 19 is placed in the carrier frame 18 as shown. Figure 3 The vacuum temperature control box cover 12 realizes the opening and closing of the vacuum temperature control box 13 in the Z-axis direction through the guide column 10 and the uncovering cylinder 9. The vacuum temperature control box cover 12 is connected with the vacuum temperature control box cover 12 through the cylinder connecting plate 8 and the vacuum temperature control box cover 12, so as to realize the movement of the vacuum temperature control box cover 12 in the Y-axis direction, so as to realize the movement of the vacuum temperature control box cover 12 in the Y-axis direction. The pipette 6 drops the detected solution on the carrier 19.

[0052] The structure of the opening and closing cylinder 9 and the slide cover assembly 7 disclosed herein is only one of many possible implementations, which is mainly used to control the opening and closing and movement of the vacuum temperature control box cover 12, so as to remove the vacuum temperature control box cover 12 and drop the solution to be tested on the carrier 19, and after dropping, the vacuum temperature control box cover is sealed with the vacuum temperature control box 13 to realize the negative pressure state of the internal environment of the vacuum assembly. Therefore, the opening and closing and movement herein can also be realized in various forms such as mechanical arm, hinge opening and closing, manual opening, etc. As long as the vacuum temperature control box 13 can be opened and closed to facilitate the carrier to enter and exit, and the structure of the sealed vacuum space belongs to the vacuum temperature control box system of the present application, and belongs to the disclosed range and protection range of the present application.

[0053] The vacuum temperature control system needs to have the ability to control the temperature of the internal environment, and needs to control the temperature in the range of-5 to 40 degrees Celsius to adapt to more solutions to be tested.

[0054] The vacuum system includes a vacuum air duct and a vacuum assembly 14, the vacuum air duct communicates the vacuum assembly 14 and the vacuum temperature control box system, the vacuum assembly 14 contains a vacuum pump, a pressure relief valve and other components, the vacuum air duct can uniformly and accurately control the air pressure in the vacuum temperature control box, so that a stable negative pressure environment is realized in the vacuum temperature control box. And can meet the vacuum degree between-0.6 to-1.0bar.

[0055] In addition to the above-mentioned components, in order to further facilitate use, a solution pretreatment machine 15 for storing the solution to be tested can also be provided. The solution pretreatment machine 15 can effectively control the initial temperature of the solution to be tested according to the different characteristics of the solution to be tested, so as to achieve better condensation effect; in order to avoid cross contamination, a disposable suction head 17 and a suction head box 16 for storing the suction head 17 can also be provided on the pipette 6.

[0056] A method for controlling the uniform crystallization of a solution on the surface of a carrier based on the device for controlling the uniform crystallization of a solution on the surface of a carrier disclosed in the present application includes the following steps:

[0057] S1, start the vacuum temperature control box system and adjust the temperature;

[0058] S2, when the temperature reaches the process requirement, open the vacuum temperature control box cover 12;

[0059] S3, place the carrier 19 into the vacuum temperature control box system;

[0060] S4, after the solution to be tested is sucked by the liquid dropping system, the solution to be tested is dropped on the surface of the carrier 19 according to the requirement of the detection process;

[0061] S5, closing the vacuum temperature control box cover 12;

[0062] S6, then starting the vacuum system, vacuuming the space in the vacuum temperature control box system, and adjusting the vacuum degree according to the process. During the vacuuming, the internal temperature of the vacuum temperature control box system will change due to the opening and closing of the cover, and the internal temperature needs to be adjusted to the set temperature again.

[0063] S7, waiting for the solution to be tested to crystallize;

[0064] S8, when the solution to be tested is crystallized on the carrier, the vacuum assembly 14 is closed;

[0065] S9, taking out the crystallized carrier 19, placing a new carrier frame 18 and carrier 19;

[0066] Exemplarily, the present application proposes an optimized crystallization method for the detection of macromolecular protein solution (the macromolecular protein described herein refers to substances such as supernatant of blood). For macromolecular protein substances, the temperature is set to a range of -5 to 25 degrees Celsius in the step of starting the vacuum temperature control box system and adjusting the temperature; and the vacuum degree is set to -0.6 to -0.8 bar in the step of then starting the vacuum system, vacuuming the space in the vacuum temperature control box system, and adjusting the vacuum degree according to the process.

[0067] Exemplarily, for small molecule proteins, the solution pretreatment machine 15 needs to be used. The solution to be tested is placed in the solution pretreatment machine 15, and the temperature of the solution to be tested is controlled to be between 10 to 35 degrees Celsius, the processing time is about 5 to 10 minutes, and it needs to be ensured that the whole solution to be tested reaches the required temperature. The temperature is set to a range of 0 to 35 degrees Celsius in the step of starting the vacuum temperature control box system and adjusting the temperature; and the vacuum degree is set to -0.7 to -1.0 bar in the step of then starting the vacuum system, vacuuming the space in the vacuum temperature control box system, and adjusting the vacuum degree according to the process. Moreover, the temperature in the vacuum temperature control box 13 (i.e. the temperature in step S1) is higher than the temperature of the pretreated solution to be tested.

[0068] In some possible embodiments, in the step of then starting the vacuum system, vacuuming the space in the vacuum temperature control box system, and adjusting the vacuum degree according to the process, air is added to the vacuum temperature control box 13 according to the properties of the solution to be tested. Exemplarily, for macromolecular proteins, a small amount of air for 1-2 seconds can be added to restore the air pressure in the vacuum temperature control box 13, so that the vacuum degree in the vacuum temperature control box 13 presents a wavy curve during the vacuuming process (as shown in Figure 4 ), which can effectively improve the success rate of uniform crystallization.

[0069] A method for controlling the uniform crystallization and equal thickness distribution of a solution on the surface of a carrier, based on the implementation method described in Embodiment 1 of the present invention, includes the following steps:

[0070] S11. First, move the Y-axis slide assembly 3, X-axis slide assembly 4, and Z-axis slide assembly 5 to the positive direction limit position. At this time, the vacuum temperature control chamber cover 12 is in the closed state. Start the vacuum temperature control chamber 13 and adjust the temperature.

[0071] S12, Y-axis slide assembly 3 and X-axis slide assembly 4 move simultaneously to the position above the tip box 16. Then, Z-axis slide assembly 5 drives pipette 6 to move downward to a set height. At this time, the outer conical surface of pipette 6 is embedded into the inner conical surface of tip 17, realizing the assembly of pipette 6 and tip 17. Then, Z-axis slide assembly 5 drives pipette 6 to move upward to a certain height.

[0072] S13. When the temperature reaches the process requirements, the cover-opening cylinder 9 installed on the cylinder connecting plate 8 works, driving the vacuum temperature control chamber cover 12 to move upward along the guide post 10, thereby opening the vacuum temperature control chamber cover 12. The vacuum temperature control chamber cover moving slide assembly 7 drives the vacuum temperature control chamber cover 12 to move backward (positive Y direction) to the limit position through the cylinder connecting plate 8.

[0073] S14. Manually remove the carrier holder 18 containing the carrier 19 (e.g., Figure 3 (As shown) Place it inside the vacuum temperature control chamber 13;

[0074] S15. After moving the X-axis slide assembly 4 to the position of the solution pretreatment machine 15, the Z-axis slide assembly 5 drives the pipette 6 and pipette tip 17 to move downward to the liquid aspiration position, starts the pipette 6 to complete the liquid aspiration work, and the Z-axis slide assembly 5 drives the pipette 6 to move upward to the safe height.

[0075] S16. Move the Y-axis slide assembly 3 and the X-axis slide assembly 4 to the position above the vacuum temperature control chamber 13 corresponding to the position of the carrier. The Z-axis slide assembly 5 drives the pipette 6 and the pipette tip 17 to move downward to the position above the carrier. Start the pipette 6 to complete the dripping operation. The Z-axis slide assembly 5 drives the pipette 6 to move upward to a safe height.

[0076] S17. The vacuum temperature control chamber cover moving slide assembly 7 runs and drives the cylinder connecting plate 8, the cover opening cylinder 9, and the vacuum temperature control chamber cover 12 forward to the position above the vacuum temperature control chamber 13. Then the cover opening cylinder 9 works and drives the vacuum temperature control chamber cover 12 to move downward along the guide post 10 to the limit position, and the vacuum temperature control chamber cover 12 closes with the vacuum temperature control chamber 13.

[0077] S18. Next, start the vacuum assembly 14, evacuate the vacuum temperature control chamber 13 through the pipeline system, and adjust the vacuum level according to the process.

[0078] S19. Solution crystallization requires a certain amount of time. At this time, move the Y-axis slide assembly 3, X-axis slide assembly 4, and Z-axis slide assembly 5 to the position above the waste pipette tip box 11, and start the pipette 6 to complete the discarding of the pipette tip.

[0079] S20, Repeat step S12;

[0080] S21. After the solution has crystallized on the carrier, turn off the vacuum component 14;

[0081] S22. Repeat step S13;

[0082] S23. Manually remove the crystallized carrier frame 18 and place a new carrier frame 18 and carrier 19.

[0083] S24. Repeat steps S15 to S23 to complete the droplet crystallization task in a cyclical manner.

[0084] Compared with the prior art, the beneficial effects of this invention are as follows: by coordinating the vacuum temperature control chamber 13, the vacuum component 14, and the solution pretreatment machine 15, and by precisely controlling the solution temperature, the working temperature inside the chamber, and the vacuum degree, the solution covering the carrier surface is crystallized and evenly distributed with the same thickness in all places, thereby improving the consistency and stability of the subsequent carrier surface sample detection process and reducing test errors and fluctuations.

[0085] Furthermore, during operation, the distribution thickness and area of ​​the sample, as well as the carrier supporting the solution, can be changed and the corresponding parameters adjusted and controlled according to actual testing needs, which improves the utilization rate and stability of the system. It can also effectively control the time of the crystallization process, the shape and size of the crystals, the thickness of the crystals, and the uniformity of the crystals, and can completely retain all the solutes in the solution, greatly reducing labor and time costs.

[0086] The equipment and method described in this invention can even be used for the crystallization of high-purity mineral water and the detection of crystallization in mineral solutions. While traditional methods leave no residue when mineral water is dripped onto the detection chip, the equipment and method of this invention clearly show crystallized residue, as shown in the figure, facilitating the detection of any remaining substances in the water. Crystallization of mineral solutions can be used to detect trace amounts of petroleum within the ore, thereby determining whether petroleum is present at that location.

[0087] At the same time, the present invention has good adaptability and can be applied to detection carriers of various sizes, as well as to the detection of various solutions other than blood samples.

[0088] Although embodiments of the present invention have been disclosed above, they are not limited to the applications listed in the specification and embodiments. It can be applied to various fields suitable for the present invention, and other modifications can be easily implemented by those skilled in the art. Therefore, without departing from the general concept defined by the claims and their equivalents, the present invention is not limited to the specific details and illustrations shown and described herein. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in this invention should be included within the protection scope of this invention.

Claims

1. A method for uniformly crystallizing a solution on a surface of a carrier to be inspected, the method using an apparatus for uniformly crystallizing a solution on a surface of a carrier to be inspected, characterized by: The device comprises a vacuum temperature control box system, a vacuum system and a droplet system, the carrier to be detected is located in the vacuum temperature control box system, the vacuum system is connected with the vacuum temperature control box system to control the vacuum degree of the vacuum temperature control box system, and the droplet system is used for dropping the solution to be detected on the carrier to be detected; the temperature adjustment range of the vacuum temperature control box system is between -5 and 40 DEG C, and the vacuum degree of the vacuum system is between -0.6 and -1.0 bar; the device further comprises a solution pretreatment machine (15), the temperature adjustment range of the solution pretreatment machine (15) is between -5 and 40 DEG C; the vacuum temperature control box system comprises a vacuum temperature control box cover (12) and a vacuum temperature control box (13); the vacuum system comprises a vacuum air duct and a vacuum component (14); the carrier to be detected is a chip for detection, silicon is used as the substrate, and micro lines or undulations are attached to the surface; The method comprises, S1: starting the vacuum temperature control box system and adjusting the temperature, so that the temperature of the vacuum temperature control box system is between -5 and 40 DEG C; S2: when the temperature reaches the process requirement, the vacuum temperature control box cover (12) is opened; S3: the carrier to be detected (19) is placed into the vacuum temperature control box system; S4: after the droplet system sucks the solution to be detected, the solution to be detected is dropped on the surface of the carrier to be detected (19) according to the detection process requirement; S5: the vacuum temperature control box cover (12) is closed; S6: then the vacuum system is started, the space in the vacuum temperature control box system is vacuumized, and the vacuum degree is controlled to be between -0.6 and -1.0 bar; in the process of vacuumizing, air is added according to the property of the solution to be detected, so that the vacuum degree of the space in the vacuum temperature control box system presents a wavy curve; S7: waiting for the solution to be detected to crystallize; S8: when the solution to be detected is crystallized on the carrier, the vacuum component (14) is closed; S9: the carrier to be detected (19) is taken out, and a new carrier frame (18) and the carrier to be detected (19) are placed.

2. The method of claim 1, wherein the solution is uniformly crystallized on the surface of the carrier to be inspected. The vacuum temperature control box (13) is a box structure and comprises a temperature adjustment device; the vacuum temperature control box cover (12) is used for controlling the sealing and opening and closing of the vacuum temperature control box system; one end of the vacuum air duct is connected with the vacuum temperature control box system, and the other end is connected with the vacuum component (14); the droplet system comprises a pipettor (6) and a moving mechanism.

3. The method of claim 2, wherein the solution is uniformly crystallized on the surface of the carrier to be inspected. The moving mechanism is an XYZ three-direction slide table assembly, a mechanical arm or a manual support.

4. The method of claim 1, wherein the solution is uniformly crystallized on the surface of the carrier to be inspected. In the step of starting the vacuum temperature control box system and adjusting the temperature, for a macromolecular protein, the temperature is set to be in the range of -5 to 25 DEG C; then the vacuum system is started, the space in the vacuum temperature control box system is vacuumized, and the vacuum degree is controlled to be between -0.6 and -0.8 bar.

5. The method of claim 1, wherein the solution is uniformly crystallized on the surface of the carrier to be inspected. For small molecule protein, the solution to be detected needs to be pretreated, the temperature of the solution to be detected is controlled between 10-35 degrees Celsius, the processing time is 5-10 minutes, the temperature in the step of starting the vacuum temperature control box system and adjusting the temperature is set in the range of 0-35 degrees Celsius; then the vacuum system is started, the space in the vacuum temperature control box system is vacuumized, and the vacuum degree is controlled between-0.7 and-1.0 bar.

6. The method of claim 5, wherein the solution is uniformly crystallized on the surface of the carrier to be inspected. The temperature in the step of starting the vacuum temperature control box system and adjusting the temperature is higher than the temperature of the pretreated solution to be detected.

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