Preparation method of ginseng powder for simultaneously increasing contents of rare ginsenosides S-Rg3, R-Rg3, Rg5, S-Rg2, R-Rg2, Rg6 and F4

Abstract

The present invention relates to a preparation method of ginseng powder for simultaneously improving the content of rare ginsenosides S-Rg3, R-Rg3, Rg5, S-Rg2, R-Rg2, Rg6 and F4, the method uses the root of Panax genus plant as raw material, powders, passes through a 40-mesh sieve, and is fully mixed with fruit juice and drinking water, wherein the amount of fruit juice is 0.2-1mL per gram of ginseng powder, the amount of drinking water is 0.1-1mL per gram of ginseng powder, is placed in a sealed container with a plug and baked, the baking temperature is 110-140°C, the baking time is 1-5h, and is taken out after cooling, and air-dried, the temperature is 25-45°C, and the air-drying time is 1-5h. When ginseng whole root powder is the raw material, the method has the advantages of simple process, green environmental protection, short processing time, low cost, no loss of effective ingredients, high content of rare ginsenosides, and can be directly eaten compared with traditional red ginseng or black ginseng production methods.

Description

Technical Field

[0001] The present invention belongs to the field of natural compounds, and relates to the structural modification of natural compounds. Specifically, it relates to a method for preparing ginseng powder rich in rare ginsenosides S-Rg3, R-Rg3, Rg5, S-Rg2, R-Rg2, Rg6, and F4. Background Art

[0002] Ginseng is the dried root of the plant of the genus Panax (Araliaceae). It has a long medicinal history in East Asian countries, especially in China and Korea. Ginseng contains ginsenosides, polysaccharides, polyphenols, nitrogen-containing compounds (proteins, amino acids, etc.), organic acids, alkaloids, vitamins and other components. Among them, ginsenosides are generally considered to be the representative components of ginseng. Research shows that by transforming the structure of ginsenosides, secondary rare ginsenosides with more excellent pharmacological activities can be obtained. For example, the secondary rare ginsenosides 20R-Rg3 and 20S-Rg3 obtained by the transformation of protopanaxadiol group ginsenosides have good anti-fatigue and immunity-enhancing effects. When used together with chemotherapy drugs, they can significantly improve the quality of life of people. Rare ginsenoside Rg5 shows good memory improvement and intelligence-enhancing effects. Rare ginsenosides Rg3 and Rg5 also have a wide range of anti-cancer, anti-inflammatory, anti-diabetic and other effects. In addition, the secondary rare ginsenoside Rg2 obtained by the transformation of protopanaxatriol group ginsenosides has the effects of anti-shock, anti-hypertension, treating vascular dementia and Alzheimer's disease. Rg6 and F4 have anti-cancer activities, and F4 also has the effect of treating cartilage degenerative diseases.

[0003] The secondary rare ginsenosides such as S-Rg3, R-Rg3, Rg5, S-Rg2, R-Rg2, Rg6, and F4 represented by Rg3 and Rg5 are present in extremely low amounts or do not exist in ginseng. Therefore, if a green, simple and low-cost method can be used to process ginseng powder rich in rare ginsenosides S-Rg3, R-Rg3, Rg5, S-Rg2, R-Rg2, Rg6, and F4, it is extremely important for the development of deep-processed ginseng products.

[0004] Red ginseng and black ginseng are popular heat-processed ginseng products after steaming, and their shapes include whole ginseng, slices, and powders. Secondary rare ginsenosides are generated during the steaming process of red ginseng and black ginseng. Therefore, red ginseng and black ginseng contain more rare ginsenosides than unprocessed ginseng, especially rare ginsenosides Rg3 and Rg5.

[0005] Although traditional red ginseng produces rare ginsenosides Rg3 and Rg5, their contents are very low. In 1983, Isao Kitagawa first isolated Rg3 from red ginseng, and 0.72 g of S-Rg3 and 0.66 g of R-Rg3 were isolated from 5 kg of red ginseng, with contents equivalent to 0.015% and 0.014% respectively, and the total was less than 3 per ten thousand (Isao Kitagawa, Masayuki Yoshikawa, Minoru Yoshihara et al. Chemical Sdudies on Crude DrugPrecession.I. On the Constituents of ginseng Radix Rubra(I). YAKUGAKU ZASSHI, 1983, 103(06):612-322). In 2005, Sang-myung Lee et al. reported the analysis results of the ginsenoside contents when making red ginseng using the traditional red ginseng production method. Among them, the content of S-Rg3 was 0.16 mg / g, the content of R-Rg3 was 0.06 mg / g, and the total was 0.22 mg / g, that is, the content was 2.2 per ten thousand (Sang-myung Lee, Bong-seok Bae, Hee-weon Park et al. JGinseng Res, 2015, 39:384-391).

[0006] Black ginseng is a heat-processed ginseng obtained by repeating the processing process of red ginseng multiple times. Therefore, the contents of ginsenosides Rg3 and Rg5 increase significantly. However, because it requires nine steaming and nine sun-drying processes (steaming for 2 h and drying in a forced-air drying oven for 8 h, repeating the same operation 8 times), its processing time is very long, reaching 90 h (Liu Chong, Preparation and Chemical Constituent Studies of Black Ginseng, Master's Thesis of Yanbian University, Yanji, 2017).

[0007] In addition, during the processing of red ginseng and black ginseng, due to the use of steam heating in a steam cabinet (suitable for large-scale production) or in a steaming basket (suitable for small-scale production), there will inevitably be a problem that the active ingredients are dissolved by steam, resulting in a decrease in the content of active ingredients. As reported by Niu Chunying et al., for every 1 kg of dry red ginseng processed, 48.6 g of dry matter in the steamed ginseng water can be obtained (Niu Chunying, Zhang Fengqing, Xie Songlin. Identification and determination of ginsenosides in steamed ginseng water. China Brewing. 2013, 32(05): 76-78), which is equivalent to a loss of about 4.86% of ginseng components. According to the research on the components of steamed ginseng water by Zhang Junchen et al., the steamed ginseng water contains water-soluble components such as sugars, saponins, proteins, amino acids, organic acids, and phenols (Zhang Junchen, Xu Yanli, Song Guangqun. Analysis of the components of steamed ginseng water [J]. Research on Chinese Patent Medicines, 1985(4): 24.). And Liu Jiyong's research on the antioxidant activities of steamed ginseng water, fresh ginseng, and red ginseng extracts shows that whether it is for scavenging DPPH, superoxide anion radicals, or hydroxyl radicals, the methanol extract of steamed ginseng water among the three different samples shows the strongest antioxidant activity, that is, its antioxidant activity is better than that of ginseng and red ginseng extracts; for the n-butanol extraction phase of steamed ginseng water, the n-butanol extraction phase of fresh ginseng, and the D101 macroporous resin water elution phase of the n-butanol extraction phase of red ginseng, DPPH radical, superoxide anion radical, and hydroxyl radical scavenging rate tests were carried out respectively, and the n-butanol extraction phase of steamed ginseng water is the strongest in terms of the scavenging rates of DPPH radicals and hydroxyl radicals (Liu Jiyong. Research on the chemical components and biological activities of steamed ginseng water. Chinese Academy of Agricultural Sciences, doctoral dissertation, 2010). Obviously, during the processing of red ginseng and black ginseng, some active ingredients with high antioxidant activity will be lost.

[0008] Therefore, it is worthy of research how to avoid the loss of active ingredients caused by steam heating and retain more ginseng components; at the same time, how to shorten the generation of secondary rare ginsenosides S-Rg3, R-Rg3, Rg5, S-Rg2, R-Rg2, Rg6, F4 through green methods, so as to improve the preparation efficiency of heat-processed ginseng and reduce costs. Summary of the Invention

[0009] In view of the above technical problems and defects, the purpose of the present invention is to provide a method for preparing ginseng powder that can simultaneously increase the contents of rare ginsenosides S-Rg3, R-Rg3, Rg5, S-Rg2, R-Rg2, Rg6, F4 and without losing active ingredients. This method uses the roots of ginseng plants as the starting material, and with the assistance of fruit juices rich in fruit acids, by reasonably controlling the process conditions, ginseng powder rich in rare ginsenosides S-Rg3, R-Rg3, Rg5, S-Rg2, R-Rg2, Rg6, F4 is obtained, and it has the advantages of simple process, environmental protection, short processing time, low cost, high content of secondary rare saponins, and can be directly eaten.

[0010] To achieve the above object, the present invention adopts the following technical solutions:

[0011] A method for preparing ginseng powder that simultaneously increases the contents of rare ginsenosides S-Rg3, R-Rg3, Rg5, S-Rg2, R-Rg2, Rg6, and F4, comprising the following steps:

[0012] Step 1. Grind dry ginseng roots and pass through a 40-mesh sieve to obtain ginseng powder for standby;

[0013] Step 2. Squeeze fresh fruits and filter through a filter cloth to obtain fruit juice for standby;

[0014] Step 3. Add ginseng powder, fruit juice, and drinking water into a container respectively and stir well; wherein, the fruit juice is added in a proportion of 0.1 - 2 mL per gram of ginseng powder, and the drinking water is added in a proportion of 0.1 - 1 mL per gram of ginseng powder;

[0015] Step 4. Bake in a closed state, with a baking temperature of 110 - 140 °C and a baking time of 1 - 5 h;

[0016] Step 5. Dry the baked and cooled ginseng powder.

[0017] Further, the ginseng in Step 1 is the root of a ginseng plant, which is a whole ginseng, main root, or fibrous root.

[0018] Further, the fruit juice in Step 2 is fruit juice extracted from a single fruit, including lemon juice, pomegranate juice, pineapple juice, grape juice; or, a mixture of fruit juices extracted from multiple single fruits; or, fruit juice extracted from multiple fruits.

[0019] Further, the ratio of the fruit juice to the ginseng powder in Step 3 is 0.4 - 1 mL / g; the ratio of the drinking water to the ginseng powder is 0.2 - 0.5 mL / g.

[0020] Further, in Step 3, the container refers to a sealed or semi-sealed container, including a hydrothermal synthesis reactor, a stoppered sealed container.

[0021] Further, in Step 4, after the ginseng powder, fruit juice, and drinking water are mixed evenly, there is no need to place them, and baking can be carried out immediately.

[0022] Further, during baking in Step 4, it is baked in a forced-air drying oven, with a baking temperature of 120 - 130 °C and a baking time of 3 - 5 h.

[0023] Further, during drying in Step 5, it is dried by forced-air drying in a forced-air drying oven, and the air-drying temperature and air-drying time are 25 - 45 °C and 1 - 5 h respectively.

[0024] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0025] 1. The method of the present invention uses the roots of Panax plants as the starting material. With the assistance of fruit juices rich in fruit acids, by reasonably controlling the process conditions, ginseng powder rich in rare ginsenosides S-Rg3, R-Rg3, Rg5, S-Rg2, R-Rg2, Rg6, and F4 is obtained, which has the advantages of simple process, environmental friendliness, short processing time, low cost, high content of secondary rare saponins, and can be directly eaten.

[0026] 2. In the present invention, after the ginseng is powdered and mixed with the fruit juice, the ginseng powder can more fully absorb the organic acids in the fruit juice, effectively increasing the content of organic acids in the ginseng, thereby improving the acid-catalysis effect and accelerating the conversion to rare ginsenosides S-Rg3, R-Rg3, Rg5, S-Rg2, Rg6, and F4.

[0027] 3. In the present invention, when ginseng, fruit juice, and water are mixed in a certain proportion, the organic acids contained in the ginseng can be better ionized to produce H + , effectively increasing the amount of H + , thereby improving its acid-catalysis effect, accelerating the generation of rare ginsenosides S-Rg3, R-Rg3, Rg5, S-Rg2, Rg6, and F4, and shortening the reaction time.

[0028] 4. The present invention uses a stoppered container and processes the ginseng powder by baking. Compared with the traditional method of steaming red ginseng, since the ginseng powder does not directly contact or has less contact with steam, the effective components can be avoided from being dissolved out by the steam, and the effective components are retained without loss.

[0029] 5. The present invention absorbs the fruit juice and water by the ginseng powder until it is in a semi-dry state, and then conducts thermal processing. With the assistance of the fruit juice, the conversion to rare ginsenosides S-Rg3, R-Rg3, Rg5, S-Rg2, Rg6, and F4 is accelerated. Compared with directly conducting thermal processing on the ginseng powder, the content of S-Rg3 is increased by about 6 times, the content of R-Rg3 is increased by about 8 - 11 times, the content of Rg5 is increased by about 7.5 - 12.5 times, the content of S-Rg2 is increased by about 1.6 - 4.6 times, the content of Rg6 is increased by about 4 - 6 times, and the content of F4 is increased by about 7 times. The addition of the fruit juice can not only increase the content of Rg3 and Rg5, but also increase the content of S-Rg2, Rg6, and F4, which is of great significance for the development of deep-processed ginseng products.

[0030] 6. Benzo[a]pyrene is a carcinogen. According to the research by Jo et al. on the production process of black ginseng, the content of benzo[a]pyrene in the medicinal materials is closely related to the temperature used during drying. As the drying temperature increases, the content of benzo[a]pyrene also increases. The most suitable drying temperature is below 50 °C (Jo E J, Kam S J, Kim E J. Effects of steam - and dry - processing temperatures on the benzo[a]pyrene content of black and red ginseng[J]. Korean J Food Nutr, 2009, 22(2):199 - 204). In the present invention, powdered roasted ginseng in a semi - dry state is used. The production rates of rare ginsenosides S - Rg3, R - Rg3, Rg5, S - Rg2, R - Rg2, Rg6, and F4 are fast. At the same time, due to the low water content and the powdered ginseng, air drying can be carried out at 25 - 45 °C, with a fast drying speed and a low temperature, effectively inhibiting the formation of benzo[a]pyrene. Description of the Drawings

[0031] Figure 1 It is the HPLC analysis chart of ginsenoside reference substances. 1 - 14 are ginsenosides Re, Rg1, S - Rg2, R - Rg2, Rb1, Rc, Rb2, Rb3, Rd, Rg6, F4, S - Rg3, R - Rg3, and Rg5 respectively.

[0032] Figure 2 It is the HPLC analysis chart of the ginseng powder extract obtained by heat - processing in Example 1 with lemon juice and water.

[0033] Figure 3 It is the HPLC analysis chart of the ginseng powder extract obtained by heat - processing in Example 2 with pomegranate juice and water.

[0034] Figure 4 It is the HPLC analysis chart of the ginseng powder extract obtained by heat - processing in Example 3 with pineapple juice and water.

[0035] Figure 5 It is the HPLC analysis chart of the ginseng powder extract obtained by heat - processing in Example 4 with grape juice and water.

[0036] Figure 6 It is the HPLC analysis chart of the ginseng powder extract directly heat - processed in Comparative Example 1. Detailed Description of the Invention

[0037] Next, the technical solutions in the embodiments of the present application will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present application.

[0038] In the present application, the reference substances of ginsenosides Re, Rg1, S-Rg2, R-Rg2, Rb1, Rc, Rb2, Rb3, Rd, Rg6, F4, S-Rg3, R-Rg3, and Rg5 were developed by Lemeitian Medicine / Dest Biotechnology, and the batch numbers and specifications are shown in Table 1.

[0039] Table 1 Specifications and Batch Numbers of Ginsenosides

[0040]

[0041]

[0042] Preparation method of ginsenoside reference substance solution: Accurately weigh 1.953 mg, 2.306 mg, 1.466 mg, 2.463 mg, 1.609 mg, 1.803 mg, 1.971 mg, 1.758 mg, 1.386 mg, 1.899 mg, 1.860 mg, 1.932 mg, 1.292 mg, and 1.560 mg of ginsenoside standards Rb1, Rb2, Rb3, Rc, Rd, Re, Rg1, S-Rg2, R-Rg2, F4, Rg6, S-Rg3, R-Rg3, and Rg5 into a 10 mL volumetric flask, and make up the volume with chromatographically pure methanol. Respectively measure 2.5 mL, 1.0 mL, 0.5 mL, 0.2 mL, and 0.05 mL, and make up the volume to 10 mL in a volumetric flask to prepare ginsenoside reference substance solutions with different concentrations.

[0043] HPLC analysis method: ODS reversed-phase chromatographic column (250 mm × 4.6 mm, 5 μm), UV detector, detection wavelength is 203 nm, injection volume is 20 μL, column temperature is 35 °C, volume flow rate is 1 mL / min. Mobile phase A is acetonitrile, and B is water. Gradient elution program: 0 - 10 min, 22% A; 10 - 25 min, 22% - 27% A; 25 - 45 min, 27% - 30% A; 45 - 55 min, 30% - 40% A; 55 - 65 min, 40% - 52% A; 65 - 72 min, 52% - 54% A; 72 - 75 min, 54% - 60% A; 75 - 77 min, 60 - 65% A; 77 - 80 min, 60 - 90% A; 80 - 95 min, 90% A; 95 - 95.10 min, 90% - 22% A; 95.10 - 102 min, 22% A.

[0044] Under the above chromatographic conditions, the reference solution was determined, and the results are as Figure 1 shown.

[0045] Example 1

[0046] This example provides a preparation method of ginseng powder that simultaneously increases the contents of rare ginsenosides S-Rg3, R-Rg3, Rg5, S-Rg2, R-Rg2, Rg6, and F4. The method includes the following steps:

[0047] Step 1: Prepare a 25 mL hydrothermal synthesis reactor;

[0048] Step 2: Grind 2 g of dry ginseng roots (whole ginseng) into ginseng powder with a mesh size of 40 - 120, and set aside;

[0049] Step 3: Juice fresh fruit (lemon) and filter it with a filter cloth to obtain lemon juice, and set aside;

[0050] Step 4: Add the ginseng powder into the liner of the hydrothermal synthesis reactor, add lemon juice according to the ratio of 0.4 mL (fruit juice) / g (ginseng powder), and then add drinking water according to the ratio of 0.2 mL (drinking water) / g (ginseng powder). Stir well to make the fruit juice and water be completely absorbed by the ginseng powder until it reaches a semi-dry state. Cover the lid of the liner, place the gasket, tighten the reactor lid, and bake it in a forced-air drying oven at 120 °C for 3 h. Cool the heat-processed ginseng to room temperature and place it in a forced-air drying oven at 45 °C for forced-air drying for 2 h.

[0051] The HPLC quantitative analysis (referring to the above chromatographic conditions) was carried out on the heat-processed ginseng powder obtained in this example after extraction. The results showed that the contents of rare ginsenosides in S-Rg3, R-Rg3, Rg5, S-Rg2, R-Rg2, Rg6, and F4 reached 0.54%, 0.34%, 0.61%, 0.11%, 0.08%, 0.08%, and 0.15% respectively, and the total content of rare ginsenosides was 1.91%. The results are shown in Figure 2 .

[0052] Example 2

[0053] This example provides a preparation method of ginseng powder that simultaneously increases the contents of rare ginsenosides S-Rg3, R-Rg3, Rg5, S-Rg2, R-Rg2, Rg6, and F4. The method includes the following steps:

[0054] Step 1: Prepare a 25 mL hydrothermal synthesis reactor;

[0055] Step 2: Grind 2 g of dry ginseng roots (whole ginseng) into ginseng powder with a mesh size of 40 - 120, and set aside;

[0056] Step 3: Juice fresh fruits (pomegranates) and filter through a filter cloth to obtain pomegranate juice, and set aside;

[0057] Step 4: Add the ginseng powder to the liner of the hydrothermal synthesis reactor, add pomegranate juice according to the ratio of 0.5 mL (fruit juice) / g (ginseng powder), and then add drinking water according to the ratio of 0.3 mL (water) / g (ginseng powder). Stir well to mix evenly so that the fruit juice and water are completely absorbed by the ginseng powder until it reaches a semi-dry state. Cover the lid of the liner, place the gasket, tighten the reactor lid, and bake it in a forced-air drying oven at 120 °C for 5 h. Cool the heat-processed ginseng to room temperature and place it in a forced-air drying oven at 45 °C for forced-air drying for 2 h.

[0058] The HPLC quantitative analysis (referring to the above chromatographic conditions) was carried out on the heat-processed ginseng powder obtained in this example after extraction; the results showed that the contents of rare ginsenosides in S-Rg3, R-Rg3, Rg5, S-Rg2, R-Rg2, Rg6, and F4 reached 0.60%, 0.43%, 0.41%, 0.15%, 0.04%, 0.11%, and 0.14% respectively, and the total content of rare ginsenosides was 1.88%. The results are shown in Figure 3 .

[0059] Example 3

[0060] This example provides a preparation method of ginseng powder that simultaneously increases the contents of rare ginsenosides S-Rg3, R-Rg3, Rg5, S-Rg2, R-Rg2, Rg6, and F4. The method includes the following steps:

[0061] Step 1. Prepare a 25 mL hydrothermal synthesis reactor;

[0062] Step 2. Grind 2 g of dried ginseng roots (whole ginseng) into ginseng powder with a mesh size of 40 - 120, and set aside;

[0063] Step 3. Juice fresh fruit (pineapple), and filter it through a filter cloth to obtain pineapple juice, and set aside;

[0064] Step 4. Add the ginseng powder into the liner of the hydrothermal synthesis reactor, add pineapple juice according to the ratio of 0.5 mL (fruit juice) / g (ginseng powder), and then add drinking water according to the ratio of 0.3 mL (water) / g (ginseng powder). Stir well to mix evenly so that the fruit juice and water are completely absorbed by the ginseng powder until it reaches a semi-dry state. Cover the lid of the liner, place the gasket, tighten the reactor lid, and bake it in a 130 °C forced air drying oven for 4 h. Cool the heat-processed ginseng to room temperature, and place it in a 45 °C forced air drying oven for air drying for 2 h.

[0065] The heat-processed ginseng powder obtained in this example was subjected to HPLC quantitative analysis (referring to the above chromatographic conditions) after extraction; the results showed that the contents of rare ginsenosides S-Rg3, R-Rg3, Rg5, S-Rg2, R-Rg2, Rg6, and F4 reached 0.51%, 0.35%, 0.38%, 0.12%, 0.06%, 0.11%, and 0.15% respectively, and the total content of rare ginsenosides was 1.68%. The results are shown in Figure 4 .

[0066] Example 4

[0067] This example provides a preparation method of ginseng powder that simultaneously increases the contents of rare ginsenosides S-Rg3, R-Rg3, Rg5, S-Rg2, R-Rg2, Rg6, and F4. The method includes the following steps:

[0068] Step 1. Prepare a 25 mL hydrothermal synthesis reactor;

[0069] Step 2. Grind 2 g of dried ginseng roots (whole ginseng) into ginseng powder with a mesh size of 40 - 120, and set aside;

[0070] Step 3. Juice fresh fruit (grape), and filter it through a filter cloth to obtain grape juice, and set aside;

[0071] Step 4. Add the ginseng powder into the liner of the hydrothermal synthesis reactor, add grape juice according to the ratio of 0.8 mL (fruit juice) / g (ginseng powder), and then add drinking water according to the ratio of 0.4 mL (water) / g (ginseng powder). Stir well to mix evenly so that the fruit juice and water are completely absorbed by the ginseng powder until it reaches a semi-dry state. Cover the lid of the liner, place the gasket, tighten the reactor lid, and bake it in a 130 °C forced air drying oven for 3 h. Cool the heat-processed ginseng to room temperature, and place it in a 45 °C forced air drying oven for air drying for 3 h.

[0072] The HPLC quantitative analysis (referring to the above chromatographic conditions) was carried out on the thermally processed ginseng powder obtained in this example after extraction; the results showed that the contents of rare ginsenosides in S-Rg3, R-Rg3, Rg5, S-Rg2, R-Rg2, Rg6, and F4 reached 0.57%, 0.37%, 0.43%, 0.32%, 0.05%, 0.11%, and 0.14% respectively, and the total content of rare ginsenosides was 1.99%. The results are shown in Figure 5 .

[0073] It should be noted that the ginseng roots in the embodiments of the present application can also be main roots or fibrous roots. After using the main roots or fibrous roots, according to the method provided in the present application, the contents of rare ginsenosides S-Rg3, R-Rg3, Rg5, S-Rg2, R-Rg2, Rg6, and F4 in the ginseng powder can also be increased.

[0074] In addition, the fruit juices of the present application include lemon juice, pomegranate juice, pineapple juice, and grape juice, but are not limited thereto. Those skilled in the art can also use other fruit juices, which can be fruit juices extracted from a single fruit, fruit juices extracted from multiple fruits, or mixtures of fruit juices extracted from multiple single fruits.

[0075] Comparative Example 1

[0076] Step 1: Prepare a 25 mL hydrothermal synthesis reactor;

[0077] Step 2: Add 2 g of ginseng powder ground to 40-120 mesh into the liner of the hydrothermal synthesis reactor, cover the lid of the liner, and bake it in a 120 °C forced air drying oven for 3 h. The thermally processed ginseng was cooled to room temperature, and HPLC quantitative analysis was carried out after extraction. The results showed that the contents of rare ginsenosides in S-Rg3, R-Rg3, Rg5, S-Rg2, R-Rg2, Rg6, and F4 reached 0.09%, 0.04%, 0.05%, 0.07%, 0.05%, 0.02%, and 0.02% respectively, and the total content of rare ginsenosides was 0.34%. The results are shown in Figure 6 .

[0078] From the results of the above examples and comparative examples, it can be seen that by thermally processing ginseng powder, the content of rare ginsenosides in ginseng powder can be increased, but the effect is not ideal; if the ginseng powder absorbs fruit juice and water to a semi-dry state and then undergoes thermal processing, with the assistance of fruit juice, the conversion to rare ginsenosides S-Rg3, R-Rg3, Rg5, S-Rg2, Rg6, and F4 is accelerated, so that the content of S-Rg3 is increased by about 6 times, the content of R-Rg3 is increased by 8-11 times, the content of Rg5 is increased by 7.5-12.5 times, the content of S-Rg2 is increased by 1.6-4.6 times, the content of Rg6 is increased by 4-6 times, and the content of F4 is increased by about 7 times.

[0079] It can be seen from this that the addition of fruit juice in this application can not only provide the contents of Rg3 and Rg5, but also significantly increase the contents of S-Rg2, Rg6, and F4, while having a relatively small impact on the content of R-Rg2.

Claims

1. A preparation method of ginseng powder for simultaneously increasing the contents of rare ginsenosides S-Rg3, R-Rg3, Rg5, S-Rg2, R-Rg2, Rg6 and F4, characterized in that It includes the following steps: Step 1. Grind dried ginseng roots into powder, sieve through a 40-mesh sieve to obtain ginseng powder, and set aside. Step 2. Squeeze fresh fruits for juice and filter with a filter cloth to obtain fruit juice, and set aside; the fruit juice is the juice extracted from a single fruit, including lemon juice, pomegranate juice, pineapple juice, and grape juice. Step 3. Add ginseng powder, fruit juice, and drinking water into a container respectively and stir well; among them, the fruit juice is added in a proportion of 0.1 - 2 mL per gram of ginseng powder, and the drinking water is added in a proportion of 0.1 - 1 mL per gram of ginseng powder. Step 4. Bake under a sealed condition, the baking temperature is 120 - 130 °C, and the baking time is 3 - 5 h. Step 5. Dry the ginseng powder after baking and cooling. When drying, it is dried by blowing air in a forced-air drying oven, and the air-drying temperature and air-drying time are 25 - 45 °C and 1 - 5 h respectively.

2. The preparation method of ginseng powder according to claim 1, characterized in that The ginseng in Step 1 is the root of a plant of the genus Panax, which is a whole ginseng, main root, or fibrous root.

3. The preparation method of ginseng powder according to claim 1, characterized in that, The ratio of the fruit juice to the ginseng powder in Step 3 is 0.4 - 1 mL / g; the ratio of the drinking water to the ginseng powder is 0.2 - 0.5 mL / g.

4. The preparation method of ginseng powder according to claim 1, characterized in that, In Step 3, the container refers to a sealed or semi-sealed container, including a hydrothermal synthesis reactor and a stoppered sealed container.

5. The preparation method of ginseng powder according to claim 1, characterized in that, In Step 4, after the ginseng powder, fruit juice, and drinking water are mixed evenly, there is no need to place them, and baking is carried out immediately.

6. The preparation method of ginseng powder according to claim 1, characterized in that, In Step 4, baking is carried out in a forced-air drying oven.

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