A method for cuttage propagation of Taraxacum kok-saghyz Rodin

Abstract

The present invention discloses a method for cutting propagation of Taraxacum kok-saghyz roots, belonging to the technical field of plant cutting. In the cutting method of the present invention, the roots of Taraxacum kok-saghyz are selected as cuttings, the cuttings are tied into bundles, left standing in a cool place for 3 - 4 h, soaked and treated with a rooting powder solution, the cuttings are inserted into the substrate, a small arch shed is covered, the temperature and humidity of the small arch shed and the substrate are controlled. After 3 - 5 days, when callus is formed, the temperature and humidity of the small arch shed and the substrate are adjusted, and rooting begins after cultivating for 5 - 7 days. Using the method of the present invention to cut the roots of Taraxacum kok-saghyz has the characteristics of simple operation process, easy to master, survival rate up to 88.9%, and short time, providing technical support for the improved variety breeding of Taraxacum kok-saghyz.

Description

Technical Field

[0001] The present invention relates to the technical field of plant cutting, and more specifically to a method for cuttage propagation of Taraxacum kok-saghyz roots. Background Art

[0002] Natural rubber, due to its excellent properties such as resilience, insulation, water isolation, and plasticity, and also having valuable properties such as oil resistance, acid resistance, alkali resistance, heat resistance, cold resistance, pressure resistance, and wear resistance after appropriate treatment, has a wide range of uses. For example, rain boots, hot water bags, and elastic bands used in daily life; surgical gloves, blood transfusion tubes, and condoms used in the medical and health industry; various tires used in transportation; conveyor belts, transport belts, acid- and alkali-resistant gloves used in industry; irrigation rubber hoses and ammonia water bags used in agriculture; sounding balloons used in meteorological measurements; sealing and shock-proof equipment used in scientific experiments; gas masks used in national defense for aircraft, tanks, and cannons; and even high-tech scientific and technological products such as rockets, artificial earth satellites, and spaceships cannot do without natural rubber.

[0003] So far, humans have discovered more than 2,900 rubber plants, including Parthenium argentatum, Taraxacum kok-saghyz, Eucommia ulmoides, and Ficus carica, etc. However, only Parthenium argentatum, Eucommia ulmoides, and Taraxacum kok-saghyz have commercial prospects due to their relatively high rubber content. Among them, Eucommia ulmoides is a small tree suitable for growing in the central temperate climate region; Parthenium argentatum is a shrub suitable for growing in tropical and subtropical semi-arid climate regions. Compared with the former two, Taraxacum kok-saghyz is a perennial herb suitable for growing in temperate and cold temperate climate regions, with a wide planting area, and can be harvested annually like crops, making it easy for mechanized production. Therefore, developing Taraxacum kok-saghyz can effectively alleviate the situation where China's natural rubber cannot meet its own needs.

[0004] With the continuous in-depth research on Taraxacum kok-saghyz, the preservation of excellent germplasm resources becomes particularly important. There are mainly two ways to propagate Taraxacum kok-saghyz, seed propagation and asexual propagation. Asexual propagation includes tissue culture and root cutting. Compared with seed propagation, although seed propagation is more economical and convenient in production practice, seed propagation will lead to significant differences in important characteristics such as rubber content and root biomass among different plants, and it is unable to effectively retain the excellent traits of the mother plant. However, asexual propagation can retain the traits of the mother plant to the greatest extent and retain the traits of excellent germplasm resources to a great extent, which is crucial for the preservation of excellent germplasm resources. Root cutting does not require strict disinfection conditions compared to tissue culture, has a simple operation process, is easy to master, and is easy to implement in production. Therefore, cuttage propagation has become the first choice for the propagation of improved varieties.

[0005] However, currently, Taraxacum kok-saghyz mainly relies on seed propagation, and there are few reports on the related cuttage breeding methods of Taraxacum kok-saghyz.

[0006] Therefore, how to provide a method for cuttage propagation of Taraxacum kok-saghyz roots is a technical problem that needs to be solved urgently by those skilled in the art. Summary of the Invention

[0007] In view of this, the present invention provides a method for cuttage propagation of Taraxacum kok-saghyz roots. The method of the present invention has the characteristics of simple operation process, easy to master, survival rate up to 88.9%, and short time, providing technical support for the breeding of improved varieties of Taraxacum kok-saghyz.

[0008] In order to achieve the above object, the present invention adopts the following technical scheme:

[0009] A method for cuttage propagation of Taraxacum kok-saghyz roots, comprising the following steps:

[0010] (1) Selection of Taraxacum kok-saghyz roots: Select healthy Taraxacum kok-saghyz roots without diseases and pests, and cut 3-5 cm stem segments in the morphological direction as cuttings.

[0011] (2) Pre-treatment before cuttage: Bundle the cuttings, leave them standing in a cool place for 3-4 h, and soak them in a rooting powder solution to obtain pre-treated cuttings.

[0012] (3) Cuttage: Insert the pre-treated cuttings into the substrate, cover with a small arch shed, control the temperature and humidity of the small arch shed and the substrate. After 3-5 days, wait for the callus to form, adjust the temperature and humidity of the small arch shed and the substrate, and start rooting after cultivating for 5-7 days.

[0013] Preferably, the cuttings are bundled as follows in step (2): Bundle 30 cuttings into one bundle; the rooting powder solution is ABT No. 1 rooting powder solution with a concentration of 300 mg / L, and the soaking treatment time is 5-7 min.

[0014] Preferably, the temperature of the cuttage in step (3) is 23-28 °C, and the depth of the cuttage is 2 / 3 of the length of the cuttings.

[0015] Preferably, the preparation method of the substrate in step (3) is as follows: Mix peat soil and perlite according to a volume ratio of 3:1, and disinfect with a carbendazim solution of 1:500 to obtain it.

[0016] Preferably, after the cuttage in step (3), the temperature of the substrate is 15-30 °C, and the humidity is 60-70%; the temperature in the small arch shed is 24-26 °C, and the humidity is 80-90%.

[0017] Preferably, after the callus forms in step (3), the temperature of the substrate is 21-24 °C, and the humidity is 50-60%; the temperature in the small arch shed is 21-24 °C, and the humidity is 60-70%.

[0018] It can be seen from the above technical solutions that compared with the prior art, the present invention has the following beneficial effects:

[0019] The present invention provides a method for cutting propagation of Taraxacum kok-saghyz roots. This cutting method has the characteristics of simple operation process, easy to master, survival rate up to 88.9%, and short time, and significantly improves the total number of roots and total number of leaves of the cuttings after cutting, providing technical support for the improved variety breeding of Taraxacum kok-saghyz. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only the embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained according to the provided drawings.

[0021] Figure 1 The drawings are: pictures of cutting substrates;

[0022] Figure 2 The drawings are: pictures of the roots of Taraxacum kok-saghyz cuttings taking root. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0023] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0024] Example 1

[0025] A method for cutting propagation of Taraxacum kok-saghyz roots includes the following steps:

[0026] (1) Selection of Taraxacum kok-saghyz roots: Select healthy Taraxacum kok-saghyz roots without diseases and pests, and cut 5-cm stem segments in the morphological direction as cuttings.

[0027] (2) Pretreatment before cutting: Bundle every 30 cuttings into a bundle, let them stand still in a cool place for 3 h, and soak them in a 300-mg / L ABT No. 1 rooting powder solution for 5 min to obtain pretreated cuttings.

[0028] (3) Cuttage: Insert the pre-treated cuttings into the substrate which is a mixture of peat soil and perlite in a volume ratio of 3:1 and disinfected with carbendazim at a ratio of 1:500 at a temperature of 23°C. The cutting depth is 3.3 cm. Cover with a small arch shed, control the temperature in the small arch shed at 24 - 26°C, the humidity at 80 - 90%, the temperature of the cutting substrate at 15 - 30°C, and the humidity at 60 - 70%. Do not uncover the small arch shed within 5 days to prevent infection by miscellaneous bacteria. After 5 days, when callus is formed, adjust the temperature in the small arch shed to 21 - 24°C, the humidity to 60 - 70%, the substrate temperature to 21 - 24°C, and the humidity to 50 - 60%. Cultivate until rooting starts on the 7th day. The picture of the cutting substrate is as shown in Figure 1 the attached drawing, and the picture of rooting is as shown in Figure 2 the attached drawing.

[0029] Example 2

[0030] A method for cuttage propagation of rubber grass roots, comprising the following steps:

[0031] (1) Selection of rubber grass roots: Select healthy rubber grass roots without diseases and pests, cut 3-cm stem segments in the morphological direction as cuttings;

[0032] (2) Pre-treatment before cuttage: Bundle every 30 cuttings into a bundle, leave them standing in a shady place for 4 h, and soak them in a 300-mg / L ABT No. 1 rooting powder solution for 7 min to obtain pre-treated cuttings;

[0033] (3) Cuttage: Insert the pre-treated cuttings into the substrate which is a mixture of peat soil and perlite in a volume ratio of 3:1 and disinfected with carbendazim at a ratio of 1:500 at a temperature of 25°C. The cutting depth is 2 cm. Cover with a small arch shed, control the temperature in the small arch shed at 24 - 26°C, the humidity at 80 - 90%, the temperature of the cutting substrate at 15 - 30°C, and the humidity at 60 - 70%. Do not uncover the small arch shed within 5 days to prevent infection by miscellaneous bacteria. After 5 days, when callus is formed, adjust the temperature in the small arch shed to 21 - 24°C, the humidity to 60 - 70%, the substrate temperature to 21 - 24°C, and the humidity to 50 - 60%. Cultivate until rooting starts on the 5th day.

[0034] Example 3

[0035] A method for cuttage propagation of rubber grass roots, comprising the following steps:

[0036] (1) Selection of rubber grass roots: Select healthy rubber grass roots without diseases and pests, cut 4-cm stem segments in the morphological direction as cuttings;

[0037] (2) Pre-treatment before cuttage: Bundle every 30 cuttings into a bundle, leave them standing in a shady place for 3 h, and soak them in a 300-mg / L ABT No. 1 rooting powder solution for 6 min to obtain pre-treated cuttings;

[0038] (3) Cuttage: Insert the pre-treated cuttings into the substrate which is a mixture of peat soil and perlite with a volume ratio of 3:1 and disinfected with carbendazim at a concentration of 1:500 at a temperature of 25°C. The depth of cutting is 2.6 cm. Cover it with a small arch shed. Control the temperature in the small arch shed at 24 - 26°C, the humidity at 80 - 90%, the temperature of the cutting substrate at 15 - 30°C, and the humidity at 60 - 70%. Do not uncover the small arch shed within 5 days to prevent infection by miscellaneous bacteria. After 5 days, when the callus is formed, adjust the temperature in the small arch shed to 21 - 24°C, the humidity to 60 - 70%, the temperature of the substrate to 21 - 24°C, and the humidity to 50 - 60%. Cultivate until roots start to grow on the 7th day.

[0039] Example 4

[0040] For the cuttage test, 3 kinds of growth regulators, namely IBA, NAA, and ABT No. 1 rooting powder, are selected, and 3 kinds of cutting substrates are used - Substrate 1 (peat soil: perlite = 3:1), Substrate 2 (perlite), and Substrate 3 (river sand). An L9(34) orthogonal test design is adopted. The 3 growth regulators are respectively set at 3 concentration gradients of 100, 300, and 500 mg / L, and the treatment time is quick dipping, 5 minutes, and 15 minutes. The test design is shown in Table 1. The remaining steps are the same as those in Example 1. There are 30 cuttings for each treatment, and it is repeated 3 times. The cuttings treated with clear water are used as the control. The rooting rate, the longest root length, the average root length, the total number of roots, and the average number of leaves of the rubber grass roots treated with different control groups are compared respectively. The experimental results are shown in Table 2.

[0041] Table 1 Design and experimental results of the rubber grass root cuttage test

[0042] Serial number Growth regulator <![CDATA[Concentration / mg·L -1 > Treatment time / min Cutting medium Survival rate 1 IBA 100 Quick dipping 1 56.7％ 2 IBA 300 5 2 68.1％ 3 IBA 500 10 3 28.9％ 4 NAA 100 Quick dipping 2 78.5％ 5 NAA 300 5 3 24.4％ 6 NAA 500 10 1 41.1％ 7 ABT1 100 Quick dipping 3 34.3％ 8 ABT1 300 5 1 88.9％ 9 ABT1 500 10 2 71.1％ CK1 Clear water - - 1, 2 or 3 0

[0043] Result analysis: As shown in Table 1, the technical solution of the present invention corresponds to the test group No. 8. It can be seen from the data in the table that compared with other control groups, the cuttage method of the present invention can significantly improve the cuttage survival rate and has significant progress.

[0044] Table 2 Effects of different treatments on various indexes of rubber grass root cuttings

[0045]

[0046]

[0047] Result analysis: As can be seen from the data in Table 2, when the technical solution of the present invention is used for cuttage of rubber gromwell roots, the cuttage rooting rate is 88.9±2.8, the longest root length is 5.5±1.0, the total number of roots is 12.3±2.9, and the average number of leaves is 14.1±2.4, showing significant differences compared with other control groups; moreover, the cuttings of the present invention can take root within 5-7 days. Compared with traditional tissue culture and the cultivation of inbred lines, it takes at least 3 months for tissue culture seedlings to form a strain system from inoculation, and 1-2 years for the cultivation of inbred lines, greatly shortening the time.

[0048] The various embodiments in this specification are described in a progressive manner. Each embodiment focuses on the differences from other embodiments. For the same and similar parts among the various embodiments, reference can be made to each other.

[0049] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be obvious to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but will be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for cutting propagation of Taraxacum kok-saghyz, characterized in that, It includes the following steps: (1) Selection of rubber grass roots: Select healthy rubber grass roots without diseases and pests, and cut 3 - 5 cm stem segments in the morphological direction as cuttings; (2) Pre - treatment before cutting: Bundle the cuttings, let them stand still in a cool place for 3 - 4 h, and then soak them in a rooting powder solution to obtain pre - treated cuttings; (3) Cutting: Insert the pre - treated cuttings into the substrate, cover with a small arch shed, control the temperature and humidity of the small arch shed and the substrate. After 3 - 5 days, wait for the callus to form, then adjust the temperature and humidity of the small arch shed and the substrate, and start rooting after cultivating for 5 - 7 days; The cuttings are bundled as follows in step (2): Bundle 30 cuttings into one bundle; the rooting powder solution is ABT No. 1 rooting powder solution with a concentration of 300 mg / L, and the soaking time is 5 - 7 min; The preparation method of the substrate in step (3) is as follows: Mix peat soil and perlite in a volume ratio of 3:1, and disinfect with a carbendazim solution of 1:500 to obtain it.

2. The rubber gromwell cutting propagation method according to claim 1, characterized in that, The temperature of cutting in step (3) is 23 - 28 °C, and the cutting depth is 2 / 3 of the cutting length.

3. The rubber gromwell cutting propagation method according to claim 1, wherein, After cutting in step (3), the substrate temperature is 15 - 30 °C, and the humidity is 60 - 70%; the temperature in the small arch shed is 24 - 26 °C, and the humidity is 80 - 90%.

4. The rubber gromwell cutting propagation method according to claim 1, characterized in that, After the callus forms in step (3), the substrate temperature is 21 - 24 °C, and the humidity is 50 - 60%; the temperature in the small arch shed is 21 - 24 °C, and the humidity is 60 - 70%.

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