An enzyme-linked immunoassay instrument and enzyme-linked immunoassay system

Abstract

The application discloses an enzyme-linked immunoassay instrument and an enzyme-linked immunoassay system, and relates to the technical field of biochemical experiment instruments, to solve the technical problems that the enzyme-linked immunoassay instrument is too large in size and difficult to carry in the prior art. The enzyme-linked immunoassay instrument comprises an optical detection component. The optical detection component comprises a light guide module and a detection module. The light guide module is configured to change the propagation direction of detection light. The detection module is arranged at one end of the light guide module from which the detection light is output. The detection module has a detection space configured to accommodate a microplate to be detected. The light guide module and the detection module are movable relative to the microplate to be detected, so as to switch the irradiation position of the detection light on the microplate to be detected. Therefore, the application has the advantages of compact structure and simple operation.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biochemical experiment instruments, in particular to an enzyme-linked immunoassay analyzer and an enzyme-linked immunoassay system. BACKGROUND

[0002] Microplate detection analyzer (also known as microplate reader) is a professional instrument commonly used in biochemical experiments in laboratories, which generally refers to an instrument for detecting the absorbance, fluorescence intensity, luminosity, etc. of a specific solution in a microplate (6-well plate to 1536-well plate). Enzyme-linked immunoassay analyzer (also known as ELISA reader) is a commonly used microplate reader, and its detection principle is enzyme-linked immunoassay. Enzyme-linked immunoassay uses enzymes coupled to antigens or antibodies to catalyze chromogenic substrates, and the reaction result is displayed by color. The absorbance value can be determined by the depth of color development, and the concentration of the antibody or antigen to be tested in the sample can be determined.

[0003] In order to make the light intensity introduced into each sample channel as uniform and consistent as possible, the existing enzyme-linked immunoassay analyzer usually uses one light source as the main light source, and achieves the above effect through light path splitting and light source movement. However, in order to let the light be guided out to the microplate in the specified direction and ensure the stability of light transmission, the existing enzyme-linked immunoassay analyzer on the market usually has a large volume and many components to be connected. The overlarge shape of the existing ELISA reader is not conducive to carrying and handling by technicians, and it also has a high requirement for the size of the workbench accommodation space, and is not suitable for placement in relatively small environments such as incubators or workstations. SUMMARY

[0004] The purpose of the present application is to provide an enzyme-linked immunoassay analyzer and an enzyme-linked immunoassay system, which can detect the absorbance of each sample in the microplate to be tested accommodated in the detection space by moving the optical detection component. The present application effectively reduces the volume of the enzyme-linked immunoassay analyzer and simplifies the overall structure, and has the advantages of compact structure and convenient operation.

[0005] The embodiments of the present application are implemented as follows:

[0006] The first aspect of the embodiments of the present application provides an enzyme-linked immunoassay analyzer, which comprises an optical detection component. The optical detection component comprises a light guide module and a detection module. The light guide module is configured to change the propagation direction of the detection light. The detection module is arranged at one end of the light guide module outputting the detection light. The detection module has a detection space configured to accommodate a microplate to be tested. The light guide module and the detection module can move relative to the microplate to be tested to switch the irradiation position of the detection light on the microplate to be tested.

[0007] In the above technical solution, the enzyme-linked immunosorbent assay (ELISA) analyzer shortens the displacement stroke required for the detection module to perform full-plate detection of the microplate by using an optical detection component that can move relative to the microplate to be tested, thereby reducing the overall size of the ELISA analyzer. In addition, the detection slots configured to accommodate the microplate to be tested, together with the interconnected light guide module and detection module, effectively improve the structural compactness of the ELISA analyzer. The relative stillness of the microplate to be tested prevents the sample solution contained within from shaking due to movement, effectively improving the accuracy and reliability of the detection results.

[0008] In one embodiment, the light guiding module includes: an optical fiber bundle and an optical fiber housing. The optical fiber bundle has a combining end and multiple branch ends; the combining end and each branch end are located on the first end face of the optical fiber housing.

[0009] In the above technical solution, the light guide module achieves the fixation of the fiber bundle and the 180-degree reversal of the light transmission direction for detection by using a bundle-combining end and multiple branching ends located on the same end face of the fiber optic housing. This solution enables the fiber to bend over short distances to change the optical path, effectively reducing the size of the light guide module.

[0010] In one embodiment, the heights of each branch end relative to the first end face are consistent.

[0011] In the above technical solution, the height of each branch end in the light guide module relative to the first end face is consistent, which facilitates the polishing of the fiber end face during the manufacturing and assembly of the light guide module and improves the consistency of the detection optical path corresponding to each branch end.

[0012] In one embodiment, each branch end is connected to the bundle end via a bent optical fiber filament, and all optical fibers are fixed within an optical fiber housing.

[0013] In the above technical solution, the light guide module improves the stability of light transmission for detection by solidifying the optical fiber filaments within the optical fiber housing. The structure, in which multiple optical fiber filaments are all solidified within the optical fiber housing without individual assembly, improves the assembly efficiency of the ELISA analyzer and effectively avoids damage to the optical fiber during the transportation and assembly of the ELISA analyzer, resulting in higher light guiding efficiency of the light guide module.

[0014] In one embodiment, the detection module includes a sample irradiation section with a detection space; one end face of the detection space is provided with a plurality of light input holes, and the other end face of the detection space is provided with a plurality of light output holes; the branch end includes a plurality of sample detection branch ends, one sample detection branch end is housed at one end of a light input hole, and the central axis of one light input hole coincides with that of one light output hole.

[0015] In the above technical solution, the detection module uses light input and output holes at both ends of the detection space to illuminate and export sample light from the sample detection branch end to each sample in the microplate within the detection space. The detection module in this solution rationally arranges space, making the overall structure more compact and allowing for independent sample channel detection of multiple sample lights with consistent intensity.

[0016] In one embodiment, the detection module further includes a sample detection plate, which is disposed at one end of the sample irradiation section and covers all light output holes.

[0017] In the above technical solution, the detection module detects the intensity of the light output after passing through the sample solution in the microplate by using a sample detection plate that covers all the light output holes.

[0018] In one embodiment, the branch end further includes a reference detection branch end, which is disposed on one side of the sample detection branch end; the detection module further includes a reference detection plate, which is connected to the first end face, and the reference detection plate is configured to detect the light intensity of the detection light output by the reference detection branch end.

[0019] In the above technical solution, the detection module uses a reference detection board to detect the intensity of the light actually output and evenly distributed by the light source module, which serves as a reference group to verify the light intensity fluctuation of the detection light and improve the accuracy and reliability of the sample absorbance detection results.

[0020] In one embodiment, the detection module further includes a plano-convex lens, which is disposed at the other end of the light input hole, with the convex surface of the plano-convex lens facing the detection space.

[0021] In the above technical solution, the detection module uses a plano-convex lens to converge the light output from the sample detection branch, so that most of the evenly distributed detection light can illuminate the sample channels in the microplate.

[0022] In one embodiment, the detection module further includes a biconvex lens, which is disposed at the end of the light output aperture near the detection space.

[0023] In the above technical solution, the detection module uses a double convex lens to converge the light rays exported through the sample channel, so that the converged light rays illuminate the sample detection plate, and the light intensity of each light ray is detected to determine the absorbance of each sample channel.

[0024] In one embodiment, the enzyme-linked immunosorbent assay (ELISA) analyzer further includes a housing and a transmission component. The microplate to be tested is fixed inside the housing; the transmission component is disposed inside the housing and is configured to move the detection module or the light guide module.

[0025] In the above technical solution, the detection module or light guide module located in the housing is moved by the transmission component to displace relative to the fixed microplate to be tested and the housing, thereby switching the irradiation position of the detection light on the microplate to be tested to detect the absorbance of the sample solution in other microwells on the microplate to be tested.

[0026] In one embodiment, the transmission component includes a synchronous belt and a motor. The synchronous belt is connected to a detection module or a light guide module; the output end of the motor is connected to the synchronous belt.

[0027] In the above technical solution, the detection module or light guide module located inside the housing moves within the housing by means of a synchronous belt driven by a motor.

[0028] In one embodiment, the transmission component further includes at least one guide rail and at least one slider. The guide rail is connected to the housing; the slider is movably disposed on the guide rail and is connected to a light guide module or a detection module.

[0029] In the above technical solution, the detection module or light guide module located inside the housing and connected to the slider, through the cooperation of the guide rail and the slider, realizes the support of the optical detection component and the guidance during movement, thereby improving the stability and reliability of the optical detection component during movement.

[0030] In one embodiment, the housing includes a microplate support, the microplate support having a microplate receiving groove and at least one snap-fit ​​member; one snap-fit ​​member is disposed at one end of the microplate receiving groove, and the microplate to be tested is detachably disposed in the microplate receiving groove through the snap-fit ​​member.

[0031] In the above technical solution, the microplate to be tested is fixed on the microplate support, and a detachable connection is achieved through the cooperation of the snap-fit ​​component and the microplate receiving groove.

[0032] In one embodiment, the optical detection component further includes a light source module, which is disposed on one side of the light guide module. The detection light output by the light source module illuminates the light receiving end face of the light guide module.

[0033] In the above technical solution, the enzyme-linked immunosorbent assay (ELISA) analyzer makes efficient use of the side space of the light guide module by mounting the light source module on one side of the light guide module, so that the detection light output can illuminate and cover the light receiving end face of the light guide module. The placement of the light source module ensures efficient use of the side space of the light guide module while maintaining high light output utilization, resulting in a more compact and rational structure for the optical detection components.

[0034] In one embodiment, the light source module includes at least one light-emitting element and at least one filter. A filter is disposed at the light output end of one of the light-emitting elements, and the filter is configured to filter out light of a specified wavelength for detection.

[0035] In the above technical solution, the light source module uses a filter located at the light output end of the light-emitting element to select the detection light that meets the requirements for detecting the absorbance of the sample solution.

[0036] In one embodiment, multiple light-emitting elements are provided, and the light emitted by each light-emitting element is filtered by a filter and converged at the light intersection point; the center point of the light receiving end face coincides with the light intersection point.

[0037] In the above technical solution, when the light source module has multiple light-emitting elements, the installation angles of the multiple light-emitting elements are reasonably arranged so that the light output by each light-emitting element can converge at the same light intersection point after being filtered by the light filter. As a result, the light receiving end face of the light guide module does not need to change its position to receive detection light with approximately equal intensity output by each light source module, thereby improving the stability of absorbance detection and the accuracy of the detection results.

[0038] In one embodiment, the light-emitting element is a monochromatic diode (LED) lamp, and the filter is a narrowband filter.

[0039] In one embodiment, the enzyme-linked immunosorbent assay (ELISA) analyzer further includes a control component, which includes a control module and a communication module. The control module is electrically connected to the detection module and the communication module, and the communication module is configured to be wired and / or wirelessly connected to a user terminal.

[0040] A second aspect of this application provides an enzyme-linked immunosorbent assay (ELISA) system, which includes at least one ELISA analyzer provided in the first aspect and any embodiment of this application, and a user terminal. The user terminal is connected to each ELISA analyzer via a communication module.

[0041] In the above technical solution, the control component, through the control module, controls the optical detection component and the communication module to receive instructions and execute absorbance detection of the microplate under test, or to send the detection data to the user terminal through the communication module, realizing data interaction and data upload. The enzyme-linked immunosorbent assay (ELISA) analyzer improves the ease of operation and automation of the detection process through the control component, effectively enhancing the user experience.

[0042] The advantages of this application compared to the prior art are:

[0043] This application provides an enzyme-linked immunosorbent assay (ELISA) analyzer and an ELISA analysis system. The analyzer uses a movable optical detection component to detect the absorbance of each microwell sample channel of a microplate containing detection slots and gradually switches the detection position. The movement of the optical detection component relative to the microplate effectively shortens the displacement required for the detection module to switch microwell detection positions, reducing the overall size of the ELISA analyzer. The fixed position of the microplate prevents liquid movement within the sample channels, reducing interference factors, improving the reliability of ELISA results, and simplifying the experimental operation. The detection slots in the optical detection component make the overall structure of the ELISA analyzer more compact. The connection between the light guide module and the detection module makes changes to the optical path and light transmission more stable, improving the reliability and accuracy of the ELISA analyzer's detection results. Attached Figure Description

[0044] To more clearly illustrate the technical solutions of the embodiments of this application, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this application and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0045] Figure 1 This is a schematic diagram of the structure of an enzyme-linked immunosorbent assay (ELISA) system according to an embodiment of this application;

[0046] Figure 2 This is a schematic diagram of the structure of an enzyme-linked immunosorbent assay (ELISA) analyzer according to an embodiment of this application;

[0047] Figure 3 This is a schematic diagram of the structure of an enzyme-linked immunosorbent assay (ELISA) analyzer in the closed state of the upper shell, as shown in an embodiment of this application;

[0048] Figure 4 This is a schematic diagram of an enzyme-linked immunosorbent assay (ELISA) analyzer with the upper shell in the open state, as shown in an embodiment of this application.

[0049] Figure 5 This is a partial structural schematic diagram of an enzyme-linked immunosorbent assay (ELISA) analyzer according to an embodiment of this application;

[0050] Figure 6 This is a schematic diagram of the overall structure of an optical detection component according to an embodiment of this application;

[0051] Figure 7 This is a schematic diagram of the overall structure of a light guide module according to an embodiment of this application;

[0052] Figure 8 This is a cross-sectional schematic diagram of a light guide module according to an embodiment of this application;

[0053] Figure 9 This is a cross-sectional schematic diagram of a detection module according to an embodiment of this application;

[0054] Figure 10 This is a cross-sectional schematic diagram of a light source module according to an embodiment of this application;

[0055] Figure 11 This is a schematic diagram of the optical path within an optical detection component according to an embodiment of this application.

[0056] Icons: 100-Microplate; 1-Enzyme-Linked Immunosorbent Assay System; 2-User Terminal; 3-Enzyme-Linked Immunosorbent Assay Analyzer; 4-Optical Detection Component; 4101-First End Face; 4102-Receiving Chamber; 410-Fiber Optic Housing; 4110-Branch End; 4111-Sample Detection Branch End; 4112-Reference Detection Branch End; 411-Fiber Bundle; 4120-Bundle Combiner End; 4121-Light Receiving End Face; 4130-Fiber Optic Wire; 41-Light Guide Module; 420-Detection Hole; 421-Mounting Hole; 422-Sample Irradiation Section; 423-Light Output Hole; 424-Light Input Hole; 425-Connector; 42-Detection Module; 430-Light Source Mounting Bracket; 431-Light Emitting Element; 432-Filter; 433-Light Crossing Point; 43-Light Source Module ; 441-Photoelectric sensor; 44-Sample detection plate; 45-Reference detection plate; 461-Lens retaining ring; 46-Biconvex lens; 47-Planar-convex lens; 5-Housing assembly; 51-Base plate; 520-Microplate receiving slot; 521-Snap-fit ​​component; 522-Silicone printing; 52-Microplate bracket; 53-Upper housing; 54-First side plate; 550-Guide groove; 551-Switch slider; 552-Limiting component; 553-Housing sensor; 554-Magnet; 55-Second side plate; 6-Transmission component; 61-Motor; 62-Synchronous belt; 63-Guide rail; 64-Slider; 71-Control module; 721-First data interface; 722-Power supply interface; 723-Second data interface; 72-Communication module; 73-Bluetooth module; 74-Switch button; 7-Control component. Detailed Implementation

[0057] The terms “first,” “second,” “third,” etc., are used only for distinguishing descriptions and do not indicate a sequence number, nor should they be interpreted as indicating or implying relative importance.

[0058] Furthermore, terms such as "horizontal," "vertical," and "sag" do not imply that components must be absolutely horizontal or suspended, but rather that they can be slightly tilted. For example, "horizontal" simply means that its direction is more horizontal relative to "vertical," and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0059] In the description of this application, it should be noted that the terms "inner", "outer", "left", "right", "upper", "lower", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product of this application is in use. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0060] In the description of this application, unless otherwise expressly specified and limited, the terms “set up,” “install,” “connect,” and “link” shall be interpreted broadly, for example, as a fixed connection, a detachable connection, or an integral connection; as a mechanical connection or an electrical connection; as a direct connection or an indirect connection through an intermediate medium; or as a connection within two components.

[0061] The technical solution of this application will now be clearly and completely described with reference to the accompanying drawings.

[0062] Please see Figure 1 , Figure 1 This is a schematic diagram illustrating the structure of an enzyme-linked immunosorbent assay (ELISA) system 1 according to an embodiment of this application. The ELISA system 1 includes at least one ELISA analyzer 3 and a user terminal 2. The ELISA analyzer 3 may have one (e.g., Figure 1 As shown in the diagram, multiple analyzers can be configured based on actual testing needs. Each enzyme-linked immunosorbent assay (ELISA) analyzer 3 establishes a communication connection with the user terminal 2 via the communication module 72. After the test is completed, the ELISA analyzer 3 sends the detected experimental data to the user terminal 2 via the communication module 72 for technicians to view. In addition, technicians can also control the operation of the ELISA analyzer 3 through the user terminal 2, including controlling the ELISA analyzer 3 to start, pause, stop, open or close the lid, etc.

[0063] User terminal 2 includes both wired and wireless terminals. User terminal 2 can be a mobile phone, tablet, or laptop computer with control software installed to control the enzyme-linked immunosorbent assay (ELISA) analyzer 3. The wireless terminal connects to the communication module 72 of the ELISA analyzer 3 via a wireless communication module for data transmission and interaction; the wired terminal connects to the ELISA analyzer 3 via a data cable for data transmission and interaction. Different types of user terminals 2 can meet different application scenarios or usage needs. Through the network connection of user terminal 2, technicians can also transmit or back up the obtained test reports to the laboratory terminal or laboratory server.

[0064] Please see Figure 2 , Figure 2This is a schematic diagram of the structure of an enzyme-linked immunosorbent assay (ELISA) analyzer 3 according to an embodiment of this application. Figure 2 As shown, this application provides an enzyme-linked immunosorbent assay (ELISA) analyzer 3, including a housing, an optical detection component 4, a transmission component 6, and a control component 7. The microplate 100 to be tested is fixed in the housing. The optical detection component 4 includes a light source module 43, a light guide module 41, and a detection module 42. The detection module 42 has a detection slot 420 for accommodating the microplate 100 to be tested. The detection module 42 is located at one end of the light guide module 41 where the detection light is output, and the light source module 43 is located on one side of the detection module 42.

[0065] The light guide module 41 is configured to change the propagation direction of the detection light. The light guide module 41 and the detection module 42 are movable relative to the microplate 100 to switch the irradiation position of the detection light on the microplate 100. The transmission component 6 is housed within the housing and configured to pull the detection module 42 or the light guide module 41. The control component 7 is located on the housing and electrically connected to the detection module 42, the light source module 43, and the transmission component 6 to control the operation or shutdown of the enzyme-linked immunosorbent assay (ELISA) analyzer 3, generate experimental data based on the data detected by the detection module 42, and interact with the user terminal 2.

[0066] During operation, the operator presets the operating parameters of the ELISA analyzer 3 using the relevant control software on the user terminal 2. Then, the top shell (i.e., upper shell 53) of the ELISA analyzer 3 is opened, and the operator installs and secures the microplate 100 to be tested in the designated position before closing the top shell (i.e., upper shell 53). The operator sends a start command for the test through the user terminal 2. Upon receiving the start command, the control component 7 controls the light source module 43 and the detection module 42 to turn on, and simultaneously controls the transmission component 6 to move relative to the connected detection module 42 or light guide module 41 and the microplate 100 to be tested, until they are moved to a position where the detection light can illuminate the microwell sample channel.

[0067] Based on the detection configuration information carried by the detection start command, the control module 71 controls the light source module 43 to light up and output detection light of a specified wavelength. The light guide module 41 divides the detection light into multiple uniform light rays with consistent light intensity and then illuminates them into some micro-well sample channels of the micro-well plate 100 to be tested. The detection module 42 detects the final output light intensity after the light passes through the sample to determine the absorbance of the sample in each micro-well sample channel.

[0068] After completing the absorbance detection of a set of sample channels, the control unit 7 moves the light guide module 41 or the detection module 42 again relative to the microplate 100 to switch the irradiation position of the detection light to other micro-sample channels of the microplate 100. Then, the ELISA analyzer 3 detects the absorbance of samples at other positions again through the detection module 42. The ELISA analyzer 3 repeats the above steps until all micro-sample channels on the microplate 100 have been detected, thus completing the full plate detection of the microplate 100.

[0069] After the test is completed, the transmission component 6 moves the optical detection component 4 back to the origin. The ELISA analyzer 3 generates an experimental report or log based on the test data and sends it to the user terminal 2. Then, the upper housing 53 of the ELISA analyzer 3 opens again. After the operator removes the tested microplate 100 from the housing, the upper housing 53 closes again, thus completing the test of one microplate 100 by the ELISA analyzer 3.

[0070] Please see Figures 3 to 5 , Figure 3 This is a schematic diagram of the structure of an enzyme-linked immunosorbent assay (ELISA) analyzer 3 in the closed state of the upper shell 53, as shown in an embodiment of this application. Figure 4 This is a schematic diagram of the structure of an enzyme-linked immunosorbent assay (ELISA) analyzer 3 with the upper housing 53 in the open state, as shown in an embodiment of this application. Figure 5 This is a partial structural schematic diagram of an enzyme-linked immunosorbent assay (ELISA) analyzer 3 according to an embodiment of this application. Please refer to... Figures 2 to 5 As shown, the housing includes a base plate 51, an upper housing 53, a first side plate 54, a second side plate 55, and a microporous plate support 52. Two first side plates 54 are provided, located at opposite ends of the base plate 51 along a first straight line direction; two second side plates 55 are provided, located at opposite ends of the base plate 51 along a second straight line direction. The first straight line direction is the direction of movement of the optical detection component 4 relative to the microporous plate 100 to be tested; the first straight line direction is perpendicular to the second straight line direction and both are horizontal.

[0071] The upper housing 53 is slidably disposed on top of the second side plate 55. When the upper housing 53 completely covers the top of the bottom plate 51, the upper housing 53, the first side plate 54, the second side plate 55, and the bottom plate 51 constitute a detection space for accommodating the optical detection component 4, the transmission component 6, and the microporous plate 100 to be tested. At this time, the upper housing 53 is in a closed state, and the two first side plates 54 precisely close the openings at both ends of the upper housing 53. The top of the second side plate 55 is provided with a guide groove 550 for accommodating the switch slider 551 and allowing the switch slider 551 to move. One end of each side wall of the upper housing 53 is connected to the second side plate 55 through the switch slider 551. Through the cooperation of the switch slider 551 and the guide groove 550, the upper housing 53 is movably disposed on the second side plate 55 to realize the open and closed states of the upper housing 53.

[0072] One end of the guide groove 550 of the second housing is provided with a limiting member 552 to limit the displacement stroke of the switch slider 551 and prevent the upper housing 53 from slipping off the second side plate 55 when it is open. A housing sensor 553 is also provided on the edge of the first side plate 54 or the edge of the second side plate 55 near the limiting member 552. When the upper housing 53 is closed, the baffle inside the upper housing 53 triggers the housing sensor 553. The control unit 7 receives the trigger signal from the housing sensor 553 and determines that the ELISA analyzer 3 is in the closed state and can continue testing. When the upper housing 53 is not closed (the upper housing 53 is not tightly closed or is open), the baffle inside the upper housing 53 does not trigger the housing sensor 553, and the control unit 7 does not receive the trigger signal from the housing sensor 553, and cannot respond to the detection start command. At this time, the ELISA analyzer 3 will send a prompt message to the user terminal 2 through the control unit 7, indicating that the upper housing 53 is not closed.

[0073] In one embodiment, the upper housing 53 is made of iron, and at least one magnet 554 is provided on the first side plate 54 near the limiting member 552. When the upper housing 53 moves and is about to close, the magnet 554 of the first side plate 54 will attract the back plate on one side of the upper housing 53, assisting the upper housing 53 to complete the closing action and seal the gap.

[0074] In one embodiment, a microplate holder 52 is fixed to a base plate 51. The microplate holder 52 has a microplate receiving groove 520 and at least one snap-fit ​​member 521. The snap-fit ​​member 521 is configured to limit and fix the microplate 100 to be tested within the microplate receiving groove 520 along a straight line. In one embodiment, one snap-fit ​​member 521 is disposed at one end of the microplate receiving groove 520 along a first straight line, and another snap-fit ​​member 521 is disposed at one end of the microplate receiving groove 520 along a second straight line. The microplate 100 to be tested is detachably disposed within the microplate receiving groove 520 via the snap-fit ​​member 521. The snap-fit ​​member 521 can be a spring, a spring-like element, or similar component.

[0075] The snap-fit ​​component 521 is fixed to the edge of the microplate receiving groove 520 by means of screws or glue. Under the opposite force of the snap-fit ​​component 521, the microplate 100 to be tested abuts against the microplate receiving groove 520 of the microplate support 52 and the snap-fit ​​component 521, thereby realizing the limiting and fixing of the microplate 100 to be tested by the microplate support 52.

[0076] During the operation, the technician places the microplate 100 to be tested into the microplate receiving groove 520 of the microplate holder 52 according to the direction indicated by the silkscreen 522 on the microplate holder 52 (i.e., the marking on the microplate 100 is aligned with the silkscreen 522). Under the reaction force of the snap-fit ​​521, the microplate 100 to be tested can be firmly fixed on the microplate holder 52. After the microplate 100 to be tested is placed, the upper housing 53 moves along the guide groove 550 towards the first side plate 54 with the switch button 74 via the switch slider 551. Since the upper housing 53 is made of iron plate, when the upper housing 53 moves to the almost closed position, the magnet 554 on the first side plate 54 at the other end of the bottom plate 51 will attract the back plate of the upper housing 53 under the action of magnetic force, completing the closing action.

[0077] When the housing sensor 553 is blocked by the baffle, it generates a corresponding trigger signal. Upon receiving this trigger signal, the ELISA analyzer 3 can continue detection. If the housing sensor 553 is not blocked and does not generate a corresponding trigger signal, the ELISA analyzer 3, in its open state, will alarm or send a prompt message to the user terminal 2 if it receives a detection start command from the user terminal 2, indicating that the instrument is not closed and cannot proceed to the next step of detection. The user should check that the cover is closed; once closed, normal detection can proceed. The housing assembly 5 effectively prevents damage to the detection module 42 caused by incomplete closure of the upper housing 53 or exposure of the detector, improving the operational reliability of the ELISA analyzer 3, extending its service life, and enhancing the user experience.

[0078] Please see Figure 6 , Figure 6 This is a schematic diagram of the overall structure of the optical detection component 4 according to an embodiment of this application. Figure 6As shown, the optical detection component 4 includes a light source module 43, a light guide module 41, and a detection module 42. The light source module 43 is located on one side of the detection module 42, and the light guide module 41 is located at one end of the detection module 42 and the light source module 43. The detection module 42 includes a sample irradiation section 422 and a sample detection plate 44, with the sample detection plate 44 located at the other end of the sample irradiation section 422 (the sample detection plate 44 and the light guide module 41 are located at opposite ends of the sample irradiation section 422). A detection slot 420 is located in the center of the sample irradiation section 422, configured to accommodate the microplate 100 to be tested. One end of the detection slot 420 of the sample irradiation section 422 has multiple light input holes 424 for irradiating the sample channel with detection light, and the other end of the detection slot 420 of the sample irradiation section 422 has multiple light output holes 423 for light absorbed by the sample, output, and conducted to the sample detection plate 44.

[0079] When the optical detection component 4 is working, the control component 7 controls the light source module 43 to output detection light of a specified wavelength. The detection light is transmitted to the light guide module 41, which changes the direction of light propagation and evenly distributes the light intensity before outputting it to the sample irradiation section 422. Multiple detection lights are output through the light guide module 41 and irradiate multiple sample channels of the microplate 100 to be tested through the light input hole 424. After the sample to be tested in the sample channel absorbs part of the detection light, the remaining light is irradiated onto the sample detection plate 44 through the sample channel and the light output hole 423. The sample detection plate 44 is provided with multiple photoelectric sensors 441 for detecting light intensity on the side near the light output hole 423. The photoelectric sensors 441 detect the light intensity of the light output through the light output hole 423 and convert it into a corresponding electrical signal. After receiving the electrical signal, the control component 7 calculates the absorbance corresponding to each sample channel.

[0080] Please see Figures 7 to 8 , Figure 7 This is a schematic diagram of the overall structure of the light guide module 41 shown in one embodiment of this application; Figure 8 This is a cross-sectional schematic diagram of a light guide module 41 according to an embodiment of this application. Please refer to... Figures 6 to 8 As shown, the light guiding module 41 includes a multi-fiber bundle 411 and an optical fiber housing 410 for housing the fiber bundle 411. The two ends of the fiber bundle 411 are located on the first end face 4101 of the optical fiber housing 410, and the middle part of the fiber bundle 411 is fixed in the receiving cavity 4102 of the optical fiber housing 410.

[0081] The fiber bundle 411 has a combining end 4120 and multiple branch ends 4110. The combining end 4120 and each branch end 4110 are located on the first end face 4101 of the fiber optic housing 410, and the height of each branch end 4110 relative to the first end face 4101 is the same. Each branch end 4110 is connected to the combining end 4120 via a bent fiber filament 4130, and all fiber filaments 4130 are cured into the fiber optic housing 410 using an encapsulation process. The branch ends 4110 include multiple sample detection branch ends 4111 and a reference detection branch end 4112, with the reference detection branch end 4112 located on one side of the sample detection branch ends 4111. In one embodiment, each branch end 4110 and the combining end 4120 are located on the top surface of the fiber optic housing 410.

[0082] In one embodiment, all sample detection branches 4111 can be uniformly arranged in one, two, or multiple columns along a first straight line to detect the corresponding number of microwell sample channels on the microplate 100. The arrangement of the sample detection branches 4111 is specifically determined according to the arrangement of the multiple microwells on the microplate 100 to be tested.

[0083] In one embodiment, the fiber bundle 411 is a 1-to-9 design, with a total fiber bending angle of 180° (achieving a 180-degree reversal of the light propagation direction for detection). The lengths of the nine fiber filaments 4130 vary depending on the bending rate of the fiber filaments 4130 and the position of each branch end 4110 relative to the bundle-combining end 4120. The size of the 9-to-1 silica fiber can be controlled within the range of 12.5 mm.

[0084] The light guide module 41 provided in this application does not use the traditional sheathing method for its optical fiber 4130. Instead, it directly uses the optical fiber housing 410 as the protective shell for the optical fiber 4130. The optical fiber 4130 of the optical fiber bundle 411 is placed in the receiving cavity 4102 of the optical fiber housing 410, which can effectively reduce the external size of the optical fiber and further reduce the bending radius of the optical fiber and the volume of the light guide module 41. This integrated design of the light guide module 41 can further reduce the size of the enzyme-linked immunosorbent assay (ELISA) analyzer 3, making it more compact and improving the consistency of detection light in each sample channel. As a modular component, the optical fiber bundle 411 can be directly solidified into the optical fiber housing 410 during the assembly process according to the positioning requirements and the bending direction of the optical path, without the need for separate assembly of each branch of the optical fiber 4130, thus improving the assembly efficiency of the ELISA analyzer 3.

[0085] The light guide module 41 uses a potting process to fix the optical fiber filaments 4130 within the receiving cavity 4102 of the optical fiber housing 410, making the light guide module 41 a cured module. Specifically, black silicone is injected into the receiving cavity 4102 where the optical fiber is fixed to cure each optical fiber filament 4130, improving the stability of the optical fiber. The black silicone can reduce optical path crosstalk caused by fiber breakage or other issues in the optical fiber bundle 411. The use of liquid black silicone to cure the optical fiber filaments 4130 effectively prevents damage to the optical fiber bundle 411 and the light guide module 41 during the transportation and assembly of the ELISA analyzer 3, improving the stability of the optical fiber. Furthermore, compared to methods such as light guide pillars, the light guide module 41, which bends the optical fiber filaments 4130 to change the direction of light transmission for detection and cures the optical fiber filaments 4130 using a potting process, has higher light guiding efficiency.

[0086] The light guide module 41 provided in this application can effectively reduce the length and volume of optical fiber, making the instrument structure more compact and small, and improving the protection level of optical fiber, which is significantly enhanced compared to traditional optical fiber protection.

[0087] The multiple branch ends 4110 and the combining end 4120 of the fiber bundle 411 are all fixed to the top surface of the fiber optic housing 410 with metal heads, and the exposed end faces of the multiple branch ends 4110 and the combining end 4120 are at the same height (h) relative to the top surface of the fiber optic housing 410. The consistent height of the exposed end faces facilitates the polishing of the fiber end faces during the manufacturing and assembly of the ELISA analyzer 3, and improves the consistency of the detection optical path corresponding to each branch end 4110.

[0088] Please see Figure 9 , Figure 9 This is a schematic cross-sectional view of the detection module 42 shown in one embodiment of this application. Please refer to... Figure 6 and Figure 9 The detection module 42 includes a sample irradiation unit 422, a sample detection plate 44, a reference detection plate 45, a biconvex lens 46, a plano-convex lens 47, and a lens retaining ring 461.

[0089] In one embodiment, the sample irradiation unit 422 has a detection slot 420; one end face of the detection slot 420 is provided with a plurality of light input holes 424, and the other end face of the detection slot 420 is provided with a plurality of light output holes 423; a sample detection branch end 4111 is housed at one end of a light input hole 424, and the central axis of the light input hole 424 coincides with that of the light output hole 423. A sample detection plate 44 is disposed at one end of the sample irradiation unit 422 and covers all the light output holes 423. A reference detection plate 45 is connected to the first end face 4101 of the optical fiber housing 410, and the reference detection plate 45 is configured to detect the light intensity of the detection light output by the reference detection branch end 4112.

[0090] In one embodiment, a plano-convex lens 47 is disposed at the other end of the light input aperture 424, with the convex surface of the plano-convex lens 47 facing the detection slot 420. A biconvex lens 46 is disposed at the end of the light output aperture 423 near the detection slot 420. A plurality of photoelectric sensors 441 are disposed on the end face of the sample detection plate 44 adjacent to the light output aperture 423, with the position of one photoelectric sensor 441 corresponding to one sample output aperture, and the photoelectric sensor 441 is configured to detect the light intensity output by the light output aperture 423.

[0091] In one embodiment, each light input aperture 424 is located at the bottom of the detection space 420, and each light output aperture 423 is located at the top of the detection space 420, directly above each light input aperture 424. The sample detection plate is fixed to the sample irradiation unit 422 by screws or the like. The sample detection plate is provided with eight photoelectric sensors 441 to measure the light intensity output from the light output aperture 423 after light absorption through each sample channel in the microplate 100 under test, and then use it for absorbance calculation.

[0092] The eight photoelectric sensors 441 on the sample detection plate 44 correspond to the eight sample detection branches 4111 of the light guide module 41. The detection light received by the combiner end 4120 of the fiber bundle 411 in the light guide module 41 is transmitted through the fiber optic cable 4130 and its optical path direction is changed, and then evenly split into nine light rays with consistent and stable light intensity. Eight sample light rays are output from the sample detection branch 4111, and one reference light ray is output from the reference detection branch 4112. The light rays output from the sample detection branch 4111 are focused by the plano-convex lens 47 in the light input hole 424 and then irradiate the multiple microwell sample channels on the microwell plate 100 to be tested, which are housed in the detection space 420. After being absorbed by the sample solution, the light rays are focused again by the biconvex lens 46 in the light output hole 423 and output to the photoelectric sensor 441, where they are converted into electrical signals for processing and analysis of the absorbance of the sample solution in each microwell.

[0093] A reference detection plate 45 is located on top of the reference detection branch 4112 of the light guide module 41. The reference light output from the reference detection branch 4112 directly illuminates the reference detection plate 45 through a small hole (the reference detection plate 45 is also equipped with a photoelectric sensor 441), and is converted into an electrical signal by the photoelectric sensor 441 on the reference detection plate 45, which is used to process and analyze the light intensity fluctuations of the reference light. The reference detection branch 4112 and the reference detection plate 45 are used to adjust the absorbance difference caused by the instability of the light source.

[0094] During the detection process, the intensity of the light emitted by the light source may decrease or increase with the detection of each row of samples, thus the absorbance calculation result may be too high or too low. Detecting the light intensity of the reference control group can effectively reduce the deviation in absorbance calculation. Since the reference light directly illuminates the photoelectric sensor 441 of the reference detection plate 45, when the light source fluctuates, the light intensity of the reference light measured by the reference detection plate 45 also changes accordingly. Therefore, the configuration of the reference detection branch 4112 and the reference detection plate 45 can effectively improve the accuracy and stability of the test results of the enzyme-linked immunosorbent assay (ELISA) analyzer 3, making the testing process more reliable.

[0095] Please see Figure 10 , Figure 10 This is a cross-sectional schematic diagram of a light source module 43 according to an embodiment of this application. Figure 10 As shown, the light source module 43 includes a light source mounting bracket 430, at least one light-emitting element 431, and at least one filter 432. A filter 432 is disposed at the light output end of one light-emitting element 431, and both the filter 432 and the light-emitting element 431 are disposed within the light source mounting bracket 430. The filter 432 is configured to filter out light of a specified wavelength for detection. Multiple light-emitting elements 431 may be provided, with each light-emitting element 431 having the same mounting angle as its corresponding filter 432, so that the angle formed between the light output from each filter 432 and the light receiving end face 4121 is equal, both being α. After being filtered by the filter 432, the light output from each light-emitting element 431 converges at the light intersection point 433, and the center point of the light receiving end face 4121 (i.e., the end face of the combining end 4120 of the fiber bundle 411 in the light guide module 41) coincides with the light intersection point 433.

[0096] In one embodiment, a mounting space 421 is provided on one side of the sample illumination section 422. The mounting space 421 is configured to mount the light source module 43 and provide space for light transmission. The light source module 43 is located in the mounting space 421 on one side of the sample illumination section 422 and at one end of the mounting space 421. The bundle-combining end 4120 of the fiber bundle 411 in the light guide module 41 is located in the mounting space 421 on one side of the sample illumination section 422 and at the other end of the mounting space 421. The detection light output by the light source module 43 illuminates the light receiving end face 4121 of the light guide module 41, which is the end face of the bundle-combining end 4120 of the fiber bundle 411.

[0097] In one embodiment, two light-emitting elements 431 and two filters 432 are provided respectively. The light-emitting elements 431 are monochromatic diodes (LEDs), and the filters 432 are narrowband filters. Each light source module 43 can output detection light of two wavelengths, and the two light-emitting elements 431 are at a certain angle. When the control unit 7 controls different light-emitting elements 431 to be lit, the light receiving end face 4121 of the light guide module 41 can receive the corresponding detection light, and transmit the detection light to the microplate 100 under test through the sample detection branch end 4111, and illuminate the sample detection plate 44 through the microplate 100 under test.

[0098] The light source module 43 is fixed to one end of the mounting slot 421 by a magnet 554. The control component 7 supplies power and controls the monochromatic diode LED lamp inside the light source module 43 through a quick-connect connector to obtain monochromatic light of the desired wavelength. The light source module 43 provided in this application can be quickly plugged in and replaced, making installation convenient and quick. When a new light source module 43 needs to be replaced, the upper housing 53 of the enzyme-linked immunosorbent assay (ELISA) analyzer 3 is switched to the open state, and the detection module 42 moves along the first linear direction to expose the light source module 43. The technician removes the old light source module 43, plugs in the new light source module 43, updates the information of the light source module 43 on the user terminal 2, and then performs relevant tests, thus completing the replacement of the light source module 43. Compared with the existing halogen lamp or xenon lamp with a filter device, the light source module 43 provided in this application has the advantages of small size, low power consumption, low heat generation, long life, and low cost.

[0099] Please see Figure 11 , Figure 11 This is a schematic diagram of the optical path within the optical detection component 4 according to an embodiment of this application. Figure 11 As shown, during an operation, the light transmission process of the ELISA analyzer 3 during detection is as follows: the light-emitting element 431 of the light source module 43 emits light, which passes through the corresponding filter 432 to filter the emitted light once, obtaining monochromatic light that meets the testing requirements (i.e., the detection light). This beam of detection light illuminates the incident end face (i.e., the light receiving end face 4121) of the light guide module 41 at a certain angle. Because the spot of the detection light is large enough, the light beam can cover all the optical fibers 4130 on the incident end face of the light guide module 41.

[0100] The light guide module 41, through the fiber bundle 411, evenly distributes and changes the direction of light transmission before transmitting it to each branch end 4110, completing the light emission. The detection light completes a 180° direction change and a 1 / 9 division transmission within a confined space. Each sample detection branch end 4111 transmits the sample light upwards to the plano-convex lens 47. The sample light passes upwards through the plano-convex lens 47 through the test material within the microporous plate 100, and then through the biconvex lens 46 above, focusing the light onto the photoelectric sensor 441 to complete the light intensity detection. The reference light is emitted from the reference detection branch end 4112 and directly illuminates the photoelectric sensor 441 on the reference detection plate 45 to monitor fluctuations in the intensity of the detection light and improve the accuracy of the absorbance test results.

[0101] During the detection process, the control unit 7 of the ELISA analyzer 3 controls the corresponding light-emitting element 431 to light up according to the preset wavelength of the detection light. Only one light-emitting element 431 is lit up during each detection. For the requirement of dual-wavelength detection light testing, the ELISA analyzer 3 performs detection light testing based on the first wavelength after the first wavelength detection light test is completed.

[0102] The light source module 43 provided in this application is located on one side of the top of the sample irradiation section 422. The light emitted by the two light-emitting elements 431 forms a certain angle and can both irradiate the incident end face of the fiber bundle 411. The light source module 43 makes full use of the side space of the detection module 42, effectively reducing the space required for light source convergence and improving the overall compactness of the instrument. The detection light output by the light source module 43 is reversed 180° and uniformly guided by the modular light guide module 41, achieving the purpose of spatial folding and dispersed transmission of the detection light.

[0103] In the optical detection component 4, the light guide module 41 and the detection module 42 are fixed together, and the light source module 43 is also installed on one side of the detection module 42 and moves with it. During the entire detection process, the light source, optical fiber and photoelectric sensor 441 have no relative displacement, and the relative positions of the optical paths evenly divided by the optical fiber do not shift. The light intensity transmission efficiency basically does not change, which can ensure the stability of the optical path.

[0104] The enzyme-linked immunosorbent assay (ELISA) analyzer 3 provided in this application has a high space utilization rate. While meeting the requirements for light transmission for detection and facilitating the replacement of the light source module 43, it can maximize the compression and utilization of the internal space of the analyzer, completing the transmission and change of light transmission direction within a small space. This application effectively reduces the overall size of the ELISA analyzer 3 and makes the components more compact, and the assembly and manufacturing cost of the ELISA analyzer 3 is also relatively low.

[0105] Please combineFigures 2 to 5 The transmission component 6 is configured to move the optical detection component 4. The transmission component 6 includes a synchronous belt 62 and a motor 61. The motor 61 is mounted on the bottom plate 51 of the housing. One end of the synchronous belt 62 is connected to the output shaft of the motor 61. When the motor 61 is turned on, the synchronous belt 62 is driven by the motor 61 to start running, and drives the detection module 42 or the light guide module 41 connected to the synchronous belt 62 to move. In one embodiment, one end of the light irradiation part of the detection module 42 is provided with a connector 425 configured to connect to the synchronous belt 62.

[0106] In other embodiments of this application, the transmission component 6 may also be driven by a motor 61, with a lead screw and nut engaging to move the optical detection component 4. The lead screw is connected to the output shaft of the motor 61 and rotates under its influence. The nut, through its engagement with the lead screw, converts the rotational motion into linear motion, causing displacement along the lead screw in a linear direction. The light guide module 41 or detection module 42, connected to the nut, moves linearly with the nut, thereby moving the optical detection component 4 relative to the microporous plate 100 to be tested.

[0107] In one embodiment, the transmission component 6 further includes at least one guide rail 63 and at least one slider 64. The guide rail 63 is disposed on the bottom plate 51 of the housing, and the slider 64 is movably disposed on the guide rail 63, and the slider 64 is connected to the bottom end of the fiber optic housing 410 of the light guide module 41. The fiber optic housing 410 of the light guide module 41 serves as a transition support. Figure 2 As shown, two guide rails 63 are provided and are arranged parallel to the linear movement direction relative to the synchronous belt 62. The support of the dual guide rails 63 and multiple sliders 64 ensures smooth sliding and stable movement of the detection module 42 during the detection of the ELISA analyzer 3. The synchronous belt 62 drives the optical detection component 4 to move along the first linear direction to realize the detection of the entire sample in the microplate 100 to be tested. The microplate 100 to be tested is stationary relative to the bottom plate 51 of the shell. When the ELISA analyzer 3 detects the sample in the microplate 100 to be tested, the sample liquid is stationary without shaking, resulting in higher testing efficiency, more accurate results, and better repeatability.

[0108] In this motion detection method, where the microplate 100 to be tested is fixed and the optical detection component 4 moves relative to the microplate 100, the displacement stroke of the optical detection component 4 is approximately equal to the length of one microplate 100 to be tested. This effectively reduces the overall size of the enzyme-linked immunosorbent assay (ELISA) analyzer 3, and the instrument has a more compact structure. The total length of the instrument is less than the sum of the lengths of two microplates 100 to be tested.

[0109] Please see Figures 2 to 5The control component 7 includes a control module 71 and a communication module 72. The control module 71 is electrically connected to the detection module 42 and the communication module 72. The communication module 72 is configured to connect to the user terminal 2 via wired and / or wireless means. The communication module 72 includes a Bluetooth module 73, a first data interface 721, and a second data interface 723. The first data interface 721 can be a USB-A data port, and the second data interface 723 can be a USB-B data port.

[0110] The control module 71 and the communication module 72 can be integrated onto a single electronic control motherboard. The control module 71 includes a core controller and a control driver, while the electronic control motherboard is fixed to the base plate 51. In one embodiment, the control component 7 further includes a switch button 74 and a power supply interface 722, which is a Type-C interface. Both the switch button 74 and the power supply interface 722 are electrically connected to the control module 71.

[0111] User terminal 2 can be divided into wired and wireless terminals. Wireless terminals, such as mobile phones or tablets equipped with instrument control software, establish a communication connection with the Bluetooth module 73 in control component 7 via Bluetooth. Wired terminals, such as PCs, can control the enzyme-linked immunosorbent assay (ELISA) analyzer 3 via instrument control software and a data cable / data interface connection. Different user terminals 2 can meet the usage needs of different scenarios or the usage habits of different technicians. Through network connectivity, user terminal 2 can transmit or back up the obtained test data or test reports to the server of the corresponding company or laboratory system, facilitating data storage and interaction.

[0112] If the ELISA analyzer 3 is in a confined space, such as an incubator or a workstation requiring a closed environment, technicians can use a wireless connection. User terminal 2 connects to the ELISA analyzer 3 via Bluetooth to set and modify the instrument's operating parameters and send test-related execution commands. After the ELISA analyzer 3 completes the test, it returns the results to the wirelessly connected user terminal 2. After sending the execution command, the ELISA analyzer 3 performs the test and temporarily backs up the test data in its own memory. User terminal 2 can then move away from the ELISA analyzer 3 or lose connection with the instrument. Once user terminal 2 returns to the instrument's Bluetooth transmission range, it re-establishes a Bluetooth connection. After Bluetooth pairing, the ELISA analyzer 3 will continuously transmit the measured data to user terminal 2.

[0113] The enzyme-linked immunosorbent assay (ELISA) analyzer 3 provided in this application has an optical detection component 4 comprising a light source module 43, a light guide module 41, and a detection module 42, all integrated into a single unit. These components move synchronously relative to the microplate 100 to switch detection positions. Throughout the detection process, there is no relative movement between the light source module 43, the optical fiber 4130, and the photoelectric sensor 441, ensuring that the light intensity transmission efficiency of the detection light remains unchanged. Therefore, the ELISA analyzer 3 is compact in size, has a compact optical path structure, is relatively inexpensive, and is easy to operate. During absorbance detection, the sample liquid within the fixed microplate 100 remains relatively still, resulting in more accurate and reliable test results and high stability of the detection process.

[0114] The enzyme-linked immunosorbent assay (ELISA) analyzer 3 provided in this application supports both wireless and wired control, meeting the usage needs of different user terminals 2 in various scenarios. The ELISA analyzer 3 eliminates the need for additional interactive hardware such as displays and button modules, effectively reducing its size and making its overall structure more compact. Users can view test reports and configure test parameters through the user terminal 2 with the instrument control software installed. While maintaining the basic functions, the assembly and manufacturing costs of the ELISA analyzer 3 are effectively reduced. The user terminal 2 can transmit or back up the acquired test reports to a server via a network connection.

[0115] This application provides an enzyme-linked immunosorbent assay (ELISA) analyzer 3 and an ELISA analysis system 1. The analyzer uses a movable optical detection component 4 to detect the absorbance of each microwell sample channel of a microplate 100 contained in detection slots 420 and gradually switches the detection position. The movement of the optical detection component 4 relative to the microplate 100 effectively shortens the displacement required for the detection module 42 to switch microwell detection positions, reducing the overall volume of the ELISA analyzer 3. The fixed position of the microplate 100 prevents liquid movement within the sample channels, reducing interference factors, improving the reliability of ELISA results, and simplifying the experimental operation. The detection slots 420 in the optical detection component 4 make the overall structure of the ELISA analyzer 3 more compact. The connection between the light guide module 41 and the detection module 42 makes changes to the optical path and light transmission more stable, improving the reliability and accuracy of the ELISA analyzer 3's detection results.

[0116] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. An enzyme-linked immunosorbent assay (ELISA) analyzer, characterized in that, include: An optical detection component, comprising a light guide module, a detection module, and a light source module; The light guide module is configured to change the propagation direction of the detection light. The detection module is located at one end of the light guide module that outputs the detection light. The detection module has a detection slot, which is configured to accommodate the microporous plate to be tested. The light guide module and the detection module can move relative to the microporous plate under test to switch the irradiation position of the detection light on the microporous plate under test; The detection module includes a sample irradiation unit, which has the detection slot; one end face of the detection slot is provided with multiple light input holes, and the other end face of the detection slot is provided with multiple light output holes. The detection module further includes a sample detection plate, which is disposed at one end of the sample irradiation section and covers all the light output holes; the sample detection plate and the light guide module are disposed at opposite ends of the sample irradiation section; The sample irradiation section is also provided with an installation space on one side, which is configured to install the light source module; the light source module is located on one side of the light guide module, and the detection light output by the light source module irradiates the light receiving end face of the light guide module. The light source module, light guide module, and detection module are assembled into one unit and are simultaneously displaced relative to the microporous plate under test to switch the detection position.

2. The enzyme-linked immunosorbent assay (ELISA) analyzer according to claim 1, characterized in that, The light guide module includes: An optical fiber bundle, the optical fiber bundle having a bundle-combining end and multiple branch ends; The optical fiber housing has the bundle-combining end and each of the branch ends located on the first end face of the optical fiber housing.

3. The enzyme-linked immunosorbent assay (ELISA) analyzer according to claim 2, characterized in that, Each of the branch ends has the same height relative to the first end face.

4. The enzyme-linked immunosorbent assay (ELISA) analyzer according to claim 2, characterized in that, Each of the branch ends is connected to the bundle end via a bent optical fiber filament, and all the optical fiber filaments are fixed within the optical fiber housing.

5. The enzyme-linked immunosorbent assay (ELISA) analyzer according to claim 2, characterized in that, The branch end includes multiple sample detection branch ends, one of the sample detection branch ends is housed at one end of one of the light input holes, and the central axis of one of the light input holes coincides with that of one of the light output holes.

6. The enzyme-linked immunosorbent assay (ELISA) analyzer according to claim 5, characterized in that, The branch end also includes a reference detection branch end, which is located on one side of the sample detection branch end; The detection module further includes a reference detection board, which is connected to the first end face. The reference detection board is configured to detect the light intensity of the detection light output from the reference detection branch.

7. The enzyme-linked immunosorbent assay (ELISA) analyzer according to claim 5, characterized in that, The detection module also includes a plano-convex lens, which is located at the other end of the light input hole, with the convex surface of the plano-convex lens facing the detection space.

8. The enzyme-linked immunosorbent assay (ELISA) analyzer according to claim 5, characterized in that, The detection module also includes a biconvex lens, which is located at one end of the light output hole near the detection space.

9. The enzyme-linked immunosorbent assay (ELISA) analyzer according to claim 1, characterized in that, The enzyme-linked immunosorbent assay (ELISA) analyzer also includes: The housing contains the microplate to be tested, which is fixed inside the housing. A transmission component, which is disposed within the housing, is configured to move the detection module or the light guide module.

10. The enzyme-linked immunosorbent assay (ELISA) analyzer according to claim 9, characterized in that, The transmission component includes: A synchronization belt, which is connected to the detection module or the light guide module; The motor, the output end of which is connected to the synchronous belt.

11. The enzyme-linked immunosorbent assay (ELISA) analyzer according to claim 10, characterized in that, The transmission component also includes: At least one guide rail is connected to the housing; At least one slider is movably disposed on the guide rail and is connected to the light guide module or the detection module.

12. The enzyme-linked immunosorbent assay (ELISA) analyzer according to claim 9, characterized in that, The housing includes a microporous plate support, which has a microporous plate receiving groove and at least one snap-fit ​​element. One of the snap-fit ​​components is disposed at one end of the microplate receiving groove, and the microplate to be tested is detachably disposed in the microplate receiving groove through the snap-fit ​​component.

13. The enzyme-linked immunosorbent assay (ELISA) analyzer according to claim 1, characterized in that, The light source module includes: At least one light-emitting element; At least one filter is disposed at the light output end of one of the light-emitting elements, and the filter is configured to filter out light of a specified wavelength as the detection light.

14. The enzyme-linked immunosorbent assay (ELISA) analyzer according to claim 13, characterized in that, The light-emitting element is provided in multiple ways. The light emitted by each light-emitting element is filtered by the filter and converges at the light intersection point. The center point of the light receiving end face coincides with the light intersection point.

15. The enzyme-linked immunosorbent assay (ELISA) analyzer according to claim 13, characterized in that, The light-emitting element is a monochrome diode LED, and the filter is a narrowband filter.

16. The enzyme-linked immunosorbent assay (ELISA) analyzer according to any one of claims 1-15, characterized in that, The enzyme-linked immunosorbent assay (ELISA) analyzer also includes: The control component includes a control module and a communication module. The control module is electrically connected to the detection module and the communication module. The communication module is configured to be wired and / or wirelessly connected to a user terminal.

17. An enzyme-linked immunosorbent assay (ELISA) system, characterized in that, The enzyme-linked reaction analysis system includes: At least one enzyme-linked immunosorbent assay (ELISA) analyzer as described in claim 16; The user terminal is connected to each of the enzyme-linked immunosorbent assay (ELISA) analyzers via the communication module.

Images