Variable volume blood cell separation post draw system and separation draw method

Abstract

The application discloses a variable-volume blood cell separation and extraction system and a separation and extraction method, and belongs to the technical field of medical devices. The system comprises a driving assembly and a centrifugal assembly. The centrifugal assembly mainly comprises a rotating disc, a supporting disc, a variable-volume bag and a centrifugal turntable. The supporting disc is located on the top of the rotating disc, and a spring is arranged between the rotating disc and the supporting disc. The centrifugal turntable is located on the top of the supporting disc and is fixed on the inner wall after being placed in the centrifugal well groove. The variable-volume bag is located in the interlayer between the supporting disc and the centrifugal turntable. The output end of the driving assembly is connected to the bottom of the rotating disc. In the separation process, the initial position before liquid inlet is back-pressured, so that the volume is variable in the whole blood centrifugal process.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of medical devices, in particular to a variable-volume blood cell separation and extraction system and a separation and extraction method. BACKGROUND

[0002] In the traditional preparation process of platelet-rich plasma, after separating whole blood in a test tube, blood cells need to be manually extracted layer by layer. This separation method cannot guarantee the concentration of the prepared platelet-rich plasma, is prone to infection, and affects the treatment effect. Moreover, the separation cup on the market cannot change the volume of the container during the extraction process after successful separation.

[0003] On the market, blood cells are extracted after separation, with red blood cells being extracted first, then plasma, and finally blood sediment brown layer. This extraction method cannot guarantee the concentration of the prepared platelet-rich plasma. Blood separation consumables are mostly bell-shaped centrifugal chambers. If blood cell separation is performed, the required rotation speed of the centrifugal cup is high, which makes blood cells prone to damage. SUMMARY

[0004] Therefore, the purpose of the present application is to provide a variable-volume blood cell separation and extraction system and a separation and extraction method. During the extraction process of the separation system, a pressure is applied to the variable-volume separation bag. When extracting the separated blood cell components, the plasma layer is extracted first, then the blood sediment brown layer, and finally the red blood cells. With the change of the extraction volume, the volume of the variable-volume bag will change.

[0005] To achieve the above purpose, the technical solution adopted by the present application is as follows:

[0006] A variable-volume blood cell separation and extraction system, comprising a driving assembly and a centrifugal assembly; the centrifugal assembly mainly comprises a rotating disc, a supporting disc, a variable-volume bag, and a centrifugal turntable;

[0007] The supporting disc is located on the top of the rotating disc, and a spring is arranged between the two; the centrifugal turntable is located above the supporting disc and is fixed to the inner wall of the centrifugal well after being placed in the groove of the centrifugal well; the variable-volume bag is located in the interlayer between the supporting disc and the centrifugal turntable;

[0008] The output end of the driving assembly is connected to the bottom of the rotating disc.

[0009] Further, the variable-volume bag and the centrifugal turntable are of an integrated structure; the center of the centrifugal turntable is provided with a center hole, and the inlet of the variable-volume bag penetrates through the center hole.

[0010] Further, it further comprises a centrifugal well; the centrifugal well is of an annular structure, is located on the top of the rotating disc, and is connected with the rotating disc through bolts; the supporting disc is located in the centrifugal well.

[0011] Further, the centrifugal turntable is arranged in a centrifugal well; an inner wall of a top port of the centrifugal well is provided with at least one arc-shaped protrusion; an outer edge of the centrifugal turntable is provided with an arc-shaped groove corresponding to the protrusion; and the centrifugal well is further provided with an annular ridge below the arc-shaped protrusion.

[0012] Further, the centrifugal turntable is further provided with an elastic clamping point; and the elastic clamping point of the centrifugal turntable is abutted against the inner wall of the centrifugal well.

[0013] Further, the centrifugal well is further provided with a clamping block; an outer wall of the centrifugal well is provided with a mounting window; and the clamping block is arranged at the mounting window; and in the fixed state, the clamping block is clamped on the arc-shaped groove of the centrifugal turntable.

[0014] Further, the clamping block comprises a force-receiving part and a force-exerting part, and the force-exerting part and the force-receiving part are connected at ends; the clamping block is connected at the mounting window through a pin shaft, and the pin shaft is parallel to a central axis of the centrifugal turntable.

[0015] Further, the driving assembly comprises a centrifugal motor, a connecting flange and a shaft; the connecting flange is connected at a bottom of the rotating disc through a bearing, and a bottom of the connecting flange is fixed on the centrifugal motor through bolts; an output end of the centrifugal motor is fixedly connected with the rotating disc through the shaft; a lower end of the spring is located in a groove of the rotating disc; a positioning pin shaft is fixed at a center of each spring, and an upper end of the spring is located in a spring groove of the supporting disc.

[0016] Further, two ends of the force-exerting part are provided with a first limiting pin shaft at a lower pressing side of an outer end, and a second limiting pin shaft is arranged at a back side of a connecting end of the force-receiving part.

[0017] A blood cell separation and extraction method is provided by the variable-volume blood cell separation and extraction system, and the specific process is as follows:

[0018] The driving assembly drives the variable-volume bag to rotate through the rotating disc and the centrifugal turntable, so that the blood cells in the variable-volume bag are centrifugally layered, and after the centrifugal layering is completed, the driving assembly still drives the rotating disc and the centrifugal turntable, and the plasma layer, the blood sedimentation brown layer and the red blood cells are sequentially extracted from inside to outside in the radial direction at the middle position of the variable-volume bag; and during the extraction process, the radial layering is still maintained with the pressure applied by the supporting disc to the variable-volume bag.

[0019] The technical scheme has the following beneficial effects:

[0020] The application can be extracted during the separation process to realize the change of the container volume. When the whole blood enters the variable volume bag, the variable volume bag volume increases, the support disc is extruded, the support disc moves downward, and the compression spring below the support disc is further compressed; after the end of the centrifugal separation, the driving assembly still drives the rotating disc and the centrifugal disc, and when the plasma layer, the blood sedimentation brown layer and the red blood cell layer obtained after the whole blood separation are extracted in turn, the compression spring rebounds, the support disc moves upward, the variable volume bag is extruded, the volume of the variable volume bag is reduced, and finally returns to the initial position before the liquid is introduced, so that the volume change during the whole blood centrifugation process is realized.

[0021] The centrifugal disc is mechanically clamped by using the clamping block, and the mechanical structure is simple and reliable. The centrifugal disc is put into the groove of the centrifugal well, and is clamped by the interaction force by rotating clockwise by 30°. The structure is simple, and the damage probability is small.

[0022] After the whole blood is separated in the variable volume bag, the inside to the outside is the plasma layer, the blood sedimentation brown layer and the red blood cell layer. The liquid taking port is located in the center of the variable volume bag, so that the extraction sequence of extracting the plasma layer first, then the blood sedimentation brown layer and finally the red blood cell layer can be realized when the blood cells after separation are extracted. The process of extracting the plasma first enables the variable volume blood cell separation and extraction system to prepare PRP according to different indications and needs of patients, and to control the platelet, neutrophil and red blood cell levels in PRP.

[0023] The liquid taking port in the center of the variable volume bag can be connected with a pump pipe. After the pump pipe is loaded, the blood layer is automatically extracted by the peristaltic pump. The automatic blood layer extraction in the closed consumables can avoid errors, microbial contamination and influence on treatment effect during manual extraction of platelet-rich plasma.

[0024] The centrifugal consumables are disc type centrifugal chambers, the centrifugal radius is large, the required rotation speed for blood cell separation is low, and the blood cell damage rate is low. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 It is a variable volume blood cell separation and extraction system overall structure diagram of the embodiment of the application;

[0026] Figure 2 It is a variable volume blood cell separation and extraction system overall structure diagram of the embodiment of the application;

[0027] Figure 3 It is a consumable clamping structure part of the embodiment of the application in the unclamped state schematic diagram;

[0028] Figure 4 It is a consumable clamping structure part of the embodiment of the application in the unclamped state schematic diagram;

[0029] Figure 5Part of the consumable clamping structure of the embodiment of the application is shown in the clamping state;

[0030] Figure 6 Part of the consumable clamping structure of the embodiment of the application is shown in the clamping state;

[0031] Figure 7 Part of the consumable clamping structure of the embodiment of the application is shown in the clamping state;

[0032] Figure 8 The electrical schematic diagram of the variable-volume blood cell separation post-extraction system of the embodiment of the application.

[0033] Figure 9 The blood cell layering state in the centrifugal state of the embodiment of the application is shown in the schematic diagram. Embodiment

[0034] The application will be further described below in combination with the drawings and specific embodiments.

[0035] In order to more clearly illustrate the technical solutions of the embodiments of the application, the drawings of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the application, and other drawings can be obtained by those skilled in the art without creative labor on the basis of these drawings.

[0036] A variable-volume blood cell separation post-extraction system, comprising a driving assembly and a centrifugal assembly; the centrifugal assembly mainly comprises a rotating disc, a supporting disc, a variable-volume bag and a centrifugal turntable;

[0037] The supporting disc is located on the top of the rotating disc, and a spring is arranged between the two; the centrifugal turntable is located above the supporting disc and is fixed to the inner wall after being placed in the centrifugal well groove; the variable-volume bag is located in the interlayer between the supporting disc and the centrifugal turntable;

[0038] The output end of the driving assembly is connected to the bottom of the rotating disc.

[0039] Further, the variable-volume bag and the centrifugal turntable are of an integrated structure; the center of the centrifugal turntable is provided with a center hole, and the inlet of the variable-volume bag is in communication with the center hole.

[0040] Further, the centrifugal well is also included; the centrifugal well is of a ring structure, is located on the top of the rotating disc and is connected to the rotating disc through bolts; and the supporting disc is located in the centrifugal well.

[0041] Further, the centrifugal turntable is arranged in a centrifugal well; an inner wall of a top port of the centrifugal well is provided with at least one arc-shaped protrusion; an outer edge of the centrifugal turntable is provided with an arc-shaped groove corresponding to the protrusion; and a ring-shaped flange is further arranged below the arc-shaped protrusion of the inner wall of the centrifugal well; and the centrifugal turntable is located between the arc-shaped protrusion and the ring-shaped flange in a fixed state.

[0042] Further, the outer edge of the centrifugal turntable is further provided with an elastic clamping point; and the elastic clamping point of the centrifugal turntable abuts against the inner wall of the centrifugal well.

[0043] Further, the centrifugal well is further provided with a clamping block; the centrifugal well is provided with a mounting window; the clamping block is located at the position of the mounting window; and the clamping block is clamped on the arc-shaped groove of the centrifugal turntable in a fixed state.

[0044] Further, the clamping block comprises a force-receiving part and a force-exerting part, and the force-exerting part and the force-receiving part are connected at the ends; the clamping block is connected at the position of the mounting window through a pin shaft, and the pin shaft is parallel to the central axis of the centrifugal turntable.

[0045] Further, the driving assembly comprises a centrifugal motor, a connecting flange and a shaft; the connecting flange is connected at the bottom of the rotating disc through a bearing, and the bottom of the connecting flange is fixed on the centrifugal motor through bolts; the output end of the centrifugal motor is fixedly connected with the rotating disc through the shaft; the lower end of the spring is located in the groove of the rotating disc; a positioning pin shaft is fixed at the center of each spring, and the upper end of the spring is located in the spring groove of the supporting disc.

[0046] Further, the outer end of the force-exerting part is provided with a first limiting pin shaft 11 at the lower pressing side, and the back side of the connecting end of the force-receiving part is provided with a second limiting pin shaft 12.

[0047] A blood cell separation and extraction method is provided by the variable-volume blood cell separation and extraction system, and the specific process is as follows:

[0048] The driving assembly drives the variable-volume bag to rotate through the rotating disc and the centrifugal turntable, so that the blood cells in the variable-volume bag are centrifuged and layered, and after the centrifugal layering is completed, the driving assembly still drives the rotating disc and the centrifugal turntable, and the plasma layer, the blood sedimentation brown layer and the red blood cells are sequentially extracted from the inside to the outside in the radial direction at the middle position of the variable-volume bag; and during the extraction process, the radial layering is still maintained with the pressure applied by the supporting disc to the variable-volume bag.

[0049] The following is a more specific embodiment:

[0050] Reference Figures 1 to 8The embodiment includes a mechanical clamping structure part, which comprises a centrifugal rotating disc 110, a consumable clamping block 120, and a centrifugal well 130; a blood cell separation variable volume part, which comprises a support disc 210, a rotating disc 220, a variable volume bag 230, and a compression spring 240; a blood cell separation transmission assembly, which comprises a connecting flange 310, a shaft 320, and a centrifugal motor 330; and a control panel 4.

[0051] The working principle of the variable volume blood cell separation post-extraction system is as follows: the centrifugal rotating disc 110 connected with the variable volume bag 230 is first placed into the annular groove on the centrifugal well 130 through the cooperation of the arc-shaped protrusions on the centrifugal well 130 and the arc-shaped grooves of the centrifugal rotating disc, at this time, the consumable clamping structure is in an unclamped state as shown in Figure 3 After the centrifugal rotating disc 110 is placed, it is rotated clockwise by 30°, at this time, the consumable clamping block 120 clamps and fixes the centrifugal rotating disc 110 to prevent it from dislocation, and the consumable clamping structure is in a clamped state as shown in Figure 5 After the equipment is started, the centrifugal motor 330 and the shaft 320 are synchronously rotated through the shaft coupling 315, the rotating disc 220 and the shaft 320 are synchronously rotated through bolt connection, and the centrifugal well 130 and the rotating disc 220 are synchronously rotated through bolt connection, and the variable volume blood cell separation post-extraction system starts to centrifuge the whole blood entering the variable volume separation bag.

[0052] The mechanical clamping structure part 1 is used to mechanically clamp the centrifugal rotating disc 110 to prevent it from dislocation during centrifugation. It comprises the centrifugal rotating disc 110, the consumable clamping block 120, and the centrifugal well 130. The overall structure diagram of the variable volume blood cell separation post-extraction system is shown in Figure 1 .

[0053] The centrifugal rotating disc 110 is used to support and fix the variable volume bag 230 so that it can rotate with the centrifugal rotating disc 110 to prevent the variable volume bag 230 from disengaging during centrifugation of the whole blood. The centrifugal rotating disc 110 is placed through the grooves between the arc-shaped protrusions on the centrifugal well 130, and after being rotated clockwise by 30°, the consumable clamping block 120 clamps and fixes the centrifugal rotating disc 110.

[0054] The consumable clamping block 120 is fixed in the consumable clamping block groove on the sidewall of the centrifugal well 130 through a pin shaft, and is used to clamp and fix the centrifugal rotating disc 110 after being rotated clockwise by 30° after being placed, as shown in Figure 5 and Figure 6 The protrusions on the inner sides of the two force applying parts and the force receiving part of the consumable clamping block 120 are circular arcs, and when the structure is clamped, the two protrusions form an interaction force to clamp the centrifugal rotating disc 110, as shown in Figure 7 .

[0055] The centrifugal well 130 is used to limit the centrifugal turntable 110 and is connected to the rotary disc 220 below it through bolt connection, so that the rotary disc 220 drives the centrifugal well 130 to rotate synchronously, and the centrifugal well 130 drives the centrifugal turntable 110 to rotate synchronously. In addition, the consumable clamping block slot on the side wall of the centrifugal well 130 can limit the consumable clamping block 120 through a rotating pin shaft and two limiting pin shafts. The limiting purpose is to limit the position of the consumable clamping block 120 and make it only rotate within a fixed angle range, thereby reducing the obstruction when the centrifugal turntable 110 is placed; refer to Figure 7 , one end of the force applying part 1 is provided with a limiting pin shaft at the side where it is pressed down, and the other end of the force applying part is provided with another limiting pin shaft at the side where it is tilted up.

[0056] The embodiment can realize two states of clamping and loosening when the centrifugal turntable 110 is loaded.

[0057] Loosening state: when the centrifugal turntable 110 is loaded into the centrifugal well 130 through the groove of the centrifugal well 130, the force applying part of the consumable clamping block 120 is pushed out, and at this time the consumable clamping block 120 is in an unclamped state. The unclamped state of the consumable clamping structure is shown in Figure 3 , and the cross-sectional view is shown in Figure 4 .

[0058] Clamping state: after the centrifugal turntable 110 is loaded into the centrifugal well 130 through the groove of the centrifugal well 130, when the centrifugal turntable 110 rotates clockwise by 30°, it pushes the consumable clamping block 120 to rotate around the rotating pin shaft, thereby clamping and positioning the centrifugal turntable 110. The two arc-shaped protrusions on the two force receiving parts and the force applying part of the consumable clamping block 120 respectively apply two central forces to the centrifugal turntable 110. According to the principle of interaction of forces, there are two forces away from the center at the contact between the centrifugal turntable 110 and the two arc-shaped protrusions of the consumable clamping block 120, thereby realizing the clamping of the centrifugal turntable 110. At this time, the consumable clamping block 120 is in a clamped state. The clamped state of the consumable clamping structure is shown in Figure 5 , and the cross-sectional view is shown in Figure 6 .

[0059] The blood cell separation variable volume part 2 is used to realize the variable volume of the centrifugal container during the whole blood centrifugation process. It includes a support disc 210, a rotary disc 220, a variable volume bag 230, and a compression spring 240. The overall structure of the variable volume blood cell separation and extraction system is shown in Figure 2 .

[0060] Supporting disc 210 is located below the variable volume bag 230. When the whole blood enters the variable volume bag 230, the variable volume bag 230 increases in volume, the supporting disc 210 is extruded, the supporting disc 210 moves downward, and the compression spring 240 below the supporting disc 210 is further compressed; when the plasma layer, the buffy coat layer and the red blood cell layer obtained after the whole blood separation are extracted in turn, the compression spring 240 rebounds, the supporting disc 210 moves upward, extruding the variable volume bag 230, so that the volume of the variable volume bag 230 decreases, and finally returns to the initial position before the liquid enters, so that the volume variable during the whole blood centrifugation process is realized.

[0061] Rotary disc 220 is located below the supporting disc 210 and is connected with the shaft 320 through bolts to rotate synchronously, and is connected with the centrifugal well 130 above through bolts to rotate synchronously. In addition, the rotary disc 220 can limit and support the compression spring 240 located thereon.

[0062] Compression spring 240 is located in the groove of the rotary disc 220 at the lower end, and a positioning pin shaft is fixed in the center of each compression spring. The upper end of the compression spring is located in the groove of the supporting disc 210, which cooperates with the supporting disc 210 and the rotary disc 220 to realize the volume variable of the blood cell separation and extraction system during the whole blood centrifugation process.

[0063] Blood cell separation transmission assembly 3 is used to drive the related components in the upper mechanical clamping structure part 1 and the blood cell separation variable volume part 2 to rotate synchronously through the transmission mechanism, so that the centrifugal assembly realizes the centrifugal function. It comprises a connecting flange 310, a shaft 320 and a centrifugal motor 330. The centrifugal motor 330 is connected with the shaft 320 through a coupling 315, the shaft 320 drives the rotary disc 220 to rotate synchronously, and the whole blood is centrifuged.

[0064] Connecting flange 310: The outer ring of the bearing 312 is limited in the bearing seat at the upper end of the connecting flange 310; the outer ring of the bearing 316 is limited in the bearing seat at the lower end of the connecting flange 310 by the axial snap spring 314; the disc spring 311 above the bearing 316 supports the bearing 312; the connecting flange 310 is fixed on the equipment upper shell through bolts.

[0065] Shaft 320: The lower end is connected with the centrifugal motor 330 through the coupling 315, and the upper end is fixed on the rotary disc 220 through bolts. Two bearings 312 are installed on the shaft 320 to reduce friction and ensure smooth operation.

[0066] Centrifugal motor 330: It is hoisted on the connecting flange 310 and connected with the shaft 320 through the coupling 315. It receives the control signal from the control panel 4 to realize the control of different directions, speeds and running time, so that the blood layer separation function of the variable volume blood cell separation and extraction system can be realized.

[0067] The control board 4 is used to convert electric signals into control signals to realize the direction, speed and time control of the centrifugal motor 330. The control board 4 only provides a schematic diagram, and its structure is not shown in the structural diagram, such as Figure 8

[0068] Referring to Figure 9 After the blood cells are centrifuged and separated, the driving assembly still drives the rotating disc, and in this state, the components in the volume bag are sequentially layered from outside to inside as red blood cells, a blood sedimentation brown layer and a plasma layer. In this state, the plasma layer located in the middle position can be directly extracted by the device.​

Claims

1. A variable volume post blood cell separation draw system comprising a drive assembly and a centrifuge assembly; characterized in that, The centrifugal assembly is mainly composed of a rotating disc, a supporting disc, a variable volume bag and a centrifugal disc; The supporting disc is located on the top of the rotating disc, and a spring is arranged between the two; The centrifugal disc is located above the supporting disc, and the variable volume bag is located between the supporting disc and the centrifugal disc; The output end of the driving assembly is connected to the bottom of the rotating disc; The variable volume bag and the centrifugal disc are in an integral structure, the center of the centrifugal disc is provided with a center hole, and the inlet of the variable volume bag is in communication with the center hole; The centrifugal well is in a ring structure, is located on the top of the rotating disc and is connected to the rotating disc through bolts; the supporting disc is located in the centrifugal well; The centrifugal disc is arranged in the centrifugal well, at least one arc-shaped protrusion is arranged on the inner wall of the top of the centrifugal well, the outer edge of the centrifugal disc is provided with an arc-shaped groove corresponding to the protrusion, and an annular flange is further arranged below the arc-shaped protrusion on the inner wall of the centrifugal well; the centrifugal disc is located between the arc-shaped protrusion and the annular flange in the fixed state; The centrifugal well is provided with a mounting window on the outer wall, the clamping block is located at the mounting window position, and the clamping block is clamped on the arc-shaped groove of the centrifugal disc in the fixed state; the clamping block comprises a force receiving part and a force applying part, and the force applying part and the force receiving part are connected at the ends; the clamping block is connected to the mounting window position through a pin shaft, the axis of the pin shaft is parallel to the central axis of the centrifugal disc, the outer end of the two ends of the force applying part is provided with a first limiting pin shaft at the lower pressing side, the back side of the connecting end of the force receiving part is provided with a second limiting pin shaft, arc-shaped protrusions are arranged on the inner sides of the force applying part and the force receiving part, and in the clamping state, the arc-shaped protrusions on the force applying part are arranged in the arc-shaped groove region, and the arc-shaped protrusions of the force receiving part are arranged in the non-arc-shaped groove region between the two arc-shaped grooves. After the centrifugal disc is loaded into the centrifugal well, the centrifugal disc is rotated by 30° clockwise, the clamping block is pushed to rotate around the pin shaft, so that the centrifugal disc is clamped and positioned.

2. A variable volume blood cell separation post draw system as defined in claim 1, wherein, The outer edge of the centrifugal disc is further provided with an elastic clamping point, and the elastic clamping point of the centrifugal disc is arranged on the inner wall of the centrifugal well.

3. A variable volume blood cell separation post draw system as in claim 1, wherein, The driving assembly comprises a centrifugal motor, a connecting flange and a shaft; the connecting flange is connected to the bottom of the rotating disc through a bearing, the bottom of the connecting flange is fixed to the centrifugal motor through bolts, the output end of the centrifugal motor is fixedly connected to the rotating disc through the shaft, the lower end of the spring is located in the groove of the rotating disc, a positioning pin shaft is fixed in the center of each spring, and the upper end of the spring is located in the spring groove of the supporting disc.

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