Composition with skin tightening effect, fermented oil, and preparation method and application thereof

Abstract

The present application relates to a composition with skin-firming efficacy, a fermented oil, and its preparation method and application. The composition with skin-firming efficacy comprises soy isoflavones, β-nicotinamide mononucleotide, and a fermentation product of Candida albicans / glucose / rapeseed oleic acid methyl ester. The composition prepared by the present application using soy isoflavones, β-nicotinamide mononucleotide, and a fermentation product of Candida albicans / glucose / rapeseed oleic acid methyl ester, in particular the fermented oil, has excellent skin-firming efficacy and can enhance the firmness and elasticity of the skin. At the same time, the above components are inexpensive, which can reduce the overall cost of cosmetics, and are easy to obtain, safe and mild, low in irritation, and have a good skin feel. In addition, the above components are all naturally derived ingredients, do not involve hormone ingredients, and can reduce skin dependence.

Description

Technical Field

[0001] The present application relates to the technical field of cosmetics, and in particular to a composition with skin firming effect, fermented oil, and a preparation method and application thereof. Background Art

[0002] The skin consists of two layers: the epidermis and the dermis. The epidermis is a stratified squamous epithelium, while the dermis is a dense connective tissue containing numerous elastic and collagen fibers, giving it elasticity and toughness. However, with aging, the body's metabolism slows, leading to a gradual loss of elastin and collagen. This reduces the number of elastic and collagen fibers in the dermis, causing the epidermis to lose support and experience uneven collapse. This leads to a decrease in skin elasticity and firmness, resulting in the appearance of wrinkles.

[0003] As people's quality of life improves, they pay more and more attention to their appearance, so it is necessary to provide cosmetics with skin firming effects. Summary of the Invention

[0004] The present application provides a composition with skin firming effect, fermented oil, and a preparation method and application thereof.

[0005] In a first aspect of the present application, a composition having skin tightening effect is provided, wherein the composition comprises soy isoflavones, β-nicotinamide mononucleotide, and a fermentation product of Candida albicans / glucose / rapeseed methyl oleate.

[0006] In one embodiment, in the composition, the mass ratio of the soy isoflavones, β-nicotinamide mononucleotide and the fermentation product of Candida albicans / glucose / rapeseed methyl oleate is (0.1-1.5):(0.1-1.5):(0.1-1.5).

[0007] The second aspect of the present application provides a fermented oil, which is prepared by mixing and fermenting the composition with skin firming effect described in the first aspect.

[0008] The third aspect of the present application provides a method for preparing the fermented oil according to the second aspect, comprising the following steps:

[0009] Mixing the components of the composition having skin firming effect to prepare an oil phase;

[0010] The oil phase is mixed with a culture medium fermentation liquid containing bacterial strains and water to carry out fermentation treatment;

[0011] The material obtained by the fermentation treatment is inactivated to prepare the fermentation oil.

[0012] In one embodiment, the bacterial strain used in the bacterial culture medium fermentation broth is Pichia pastoris, and the culture medium is MRS broth medium.

[0013] In one embodiment, the mass ratio of the oil phase to the culture medium fermentation broth containing the bacterial strain and water is (0.1-2):(0.1-1):(1-10).

[0014] In one embodiment, the fermentation treatment conditions include: a temperature of 30° C. to 50° C., a stirring speed of 150 rpm to 250 rpm, and a time of 80 h to 120 h.

[0015] In one embodiment, the mixing conditions of the components include: a temperature of 50° C. to 80° C., a stirring speed of 30 rpm to 250 rpm, and a mixing time of 10 min to 40 min.

[0016] The fourth aspect of the present application provides use of the composition with skin firming effect described in the first aspect or the fermented oil described in the second aspect in the preparation of cosmetics with skin firming effect.

[0017] In a fifth aspect of the present application, a cosmetic is provided, comprising the composition with skin firming effect described in the first aspect or the fermented oil described in the second aspect.

[0018] In one embodiment, the cosmetic comprises a cosmetic base and the fermented oil, and the fermented oil accounts for 1% to 10% by mass in the cosmetic.

[0019] The composition prepared in this application by combining soy isoflavones, β-nicotinamide mononucleotide and Candida albicans / glucose / rapeseed methyl ester fermentation products, especially the fermented oil, has excellent skin firming effect and can improve skin firmness and elasticity.

[0020] These ingredients are also inexpensive, reducing the overall cost of cosmetics. They are also readily available, safe, mild, low-irritating, and pleasant to the skin. Furthermore, they are all naturally derived and contain no hormones, reducing skin dependency. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 The skin firmness F4 value test result of an embodiment of the present application;

[0022] Figure 2 The skin elasticity R2 test result of an embodiment of the present application;

[0023] Figure 3 This is the skin elasticity R5 test result of an embodiment of the present application;

[0024] Figure 4 This is the skin elasticity R7 test result of an embodiment of the present application. DETAILED DESCRIPTION

[0025] The following detailed description of the skin-tightening composition, fermented oil, preparation method, and application of the present application is provided with reference to specific examples. This application can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to facilitate a more thorough and comprehensive understanding of the present disclosure.

[0026] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application pertains. The terms used herein in the specification of this application are for the purpose of describing specific embodiments only and are not intended to limit this application.

[0027] In this application, terms such as "first aspect," "second aspect," "third aspect," "fourth aspect," and "fifth aspect" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or quantity, nor should they be understood as implicitly indicating the importance or quantity of the technical features indicated. Furthermore, terms such as "first," "second," "third," "fourth," and "fifth" serve only as non-exhaustive enumeration and description, and should not constitute a closed-ended limitation on quantity.

[0028] In this application, the technical features described in an open manner include closed technical solutions composed of the listed features, and also include open technical solutions containing the listed features.

[0029] In this application, when referring to numerical ranges, unless otherwise specified, the numerical ranges are considered continuous and include the minimum and maximum values ​​of the range, as well as every value between such minimum and maximum values. Further, when a range refers to an integer, every integer between the minimum and maximum values ​​of the range is included. In addition, when multiple ranges are provided to describe a feature or characteristic, the ranges can be combined. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges subsumed therein.

[0030] Unless otherwise specified, the percentage contents mentioned in this application refer to mass percentage for solid-liquid mixture and solid-solid mixture, and refer to volume percentage for liquid-liquid mixture.

[0031] The percentage concentrations mentioned in this application, unless otherwise specified, refer to the final concentration, which refers to the percentage of the added component in the system after the addition of the component.

[0032] The temperature parameters in this application, unless otherwise specified, allow for both constant temperature treatment and treatment within a certain temperature range. The constant temperature treatment allows for temperature fluctuations within the precision range of instrument control.

[0033] The room temperature in this application generally refers to 4°C to 30°C, preferably 20±5°C.

[0034] Soy isoflavones are a class of flavonoid compounds with a similar structure to estrogen, known as "natural phytoestrogens." They possess clear antioxidant properties, effectively inhibiting the production of oxygen free radicals, hydrogen peroxide, DNA oxidative damage, and lipid peroxidation. They also promote collagen production, keeping skin soft and smooth, and reducing wrinkles caused by sagging skin.

[0035] β-Nicotinamide mononucleotide (NMN) is a precursor for NAD+ synthesis in the human body, indirectly or directly replenishing NAD+ for skin tissue and epidermal cells. Because NAD+ is an essential coenzyme component for hundreds of enzymes in the human body, including longevity enzymes, NAD+ levels gradually decrease with age, impairing communication between mitochondria and the cell nucleus. This reduction in NAD+ also impairs the cell's ability to produce energy, contributing to aging and disease. The addition of NMN can significantly enhance cell activity, thereby delaying, improving, and preventing aging.

[0036] The fermentation product of Candida albicans / glucose / rapeseed methyl ester can enable the collagen composition to penetrate deeply into the skin, improve the permeability of the collagen composition, regulate the skin's water and oil balance, soften the cuticle, improve the skin's ability to absorb collagen, maintain cell elasticity, promote the regeneration of damaged tissues, improve the skin's repair ability, and delay cell aging, thereby maintaining skin elasticity and improving the skin's anti-aging ability. It can also effectively improve the skin's antibacterial properties and reduce skin sensitivity.

[0037] So far, there has been no research on the synergistic effects among soy isoflavones, β-nicotinamide mononucleotide, and Candida albicans / glucose / rapeseed methyl oleate fermentation products.

[0038] Through research, the present application discovered that there is a synergistic effect between soy isoflavones, β-nicotinamide mononucleotide, and the fermentation product of Candida albicans / glucose / rapeseed methyl ester. The product prepared by combining the three, especially the fermented oil, can enhance the firmness and elasticity of the skin and achieve excellent skin firming effect.

[0039] Based on this, some examples of the present application provide a composition with skin tightening effect, which includes soy isoflavones, β-nicotinamide mononucleotide and Candida albicans / glucose / rapeseed methyl ester fermentation product.

[0040] The composition, prepared by combining soy isoflavones, β-nicotinamide mononucleotide, and a fermentation product of Candida albicans, glucose, and rapeseed methyl ester, exhibits excellent skin-tightening properties, particularly the fermented oil. These components are inexpensive, reducing the overall cost of cosmetics. They are also readily available, safe, mild, low-irritation, and possess a pleasant skin feel. Furthermore, all of these components are naturally derived, free of hormonal ingredients, thus reducing skin dependency.

[0041] In some examples, in the composition, the mass ratio of soy isoflavones, β-nicotinamide mononucleotide, and the fermentation product of Candida albiflora / glucose / rapeseed methyl oleate is (0.1-1.5):(0.1-1.5):(0.1-1.5). Furthermore, the mass ratio of soy isoflavones, β-nicotinamide mononucleotide, and the fermentation product of Candida albiflora / glucose / rapeseed methyl oleate is (0.5-1.5):(0.5-1.5):(0.5-1.5). Furthermore, the mass ratio of soy isoflavones, β-nicotinamide mononucleotide, and the fermentation product of Candida albiflora / glucose / rapeseed methyl oleate is (0.8-1.2):(0.8-1.2):(0.8-1.2). Furthermore, the mass ratio of soy isoflavones, β-nicotinamide mononucleotide, and the fermentation product of Candida albiflora / glucose / rapeseed methyl oleate is 1:1:1.

[0042] Other examples of the present application provide a fermented oil, which is prepared by mixing and fermenting the composition with skin firming effect as described above.

[0043] Other examples of the present application provide a method for preparing the fermented oil as described above, comprising the following steps:

[0044] Mixing the components of the composition having skin firming effect to prepare an oil phase;

[0045] The oil phase is mixed with a culture medium fermentation liquid containing bacterial strains and water to carry out fermentation treatment;

[0046] The material obtained by the fermentation treatment is inactivated to prepare the fermentation oil.

[0047] In some examples, the bacterial strain used in the bacterial culture medium fermentation broth is Pichia pastoris.

[0048] In some examples, the culture medium used in the culture medium fermentation broth containing the bacterial strain is MRS broth medium.

[0049] Without limitation, the method for preparing the culture medium fermentation broth containing the bacterial strain comprises the following steps:

[0050] The culture medium is inoculated with the bacterial strain for fermentation, and then the fermentation product is filtered and the filtrate is collected, which is the culture medium fermentation liquid containing the bacterial strain.

[0051] In some examples, the mass percentage of the bacterial species is 2% to 6% based on the mass of the culture medium.

[0052] In some examples, the conditions for inoculating the culture medium with the bacteria and performing fermentation include: a temperature of 30° C. to 40° C. and a fermentation time of 10 h to 15 h.

[0053] In some examples, the mass ratio of the oil phase to the culture medium fermentation broth containing the bacterial strain and water is (0.1-2):(0.1-1):(1-10). Furthermore, the mass ratio of the oil phase to the culture medium fermentation broth containing the bacterial strain and water is (0.5-1.5):(0.3-0.7):(3-7). Furthermore, the mass ratio of the oil phase to the culture medium fermentation broth containing the bacterial strain and water is 1:0.5:5.

[0054] In some examples, fermentation conditions include: a temperature of 30°C to 50°C, a stirring speed of 150 rpm to 250 rpm, and a fermentation time of 80 hours to 120 hours. Without limitation, fermentation temperatures include, but are not limited to, 30°C, 35°C, 40°C, 45°C, and 50°C; stirring speeds include, but are not limited to, 150 rpm, 180 rpm, 200 rpm, 220 rpm, and 250 rpm; and fermentation times include, but are not limited to, 80 hours, 85 hours, 90 hours, 95 hours, 100 hours, 105 hours, 110 hours, 115 hours, and 120 hours.

[0055] In some examples, the conditions for mixing the components include: a temperature of 50°C to 80°C, a stirring speed of 30 rpm to 250 rpm, and a time of 10 min to 40 min. Without limitation, the temperatures for mixing the components include, but are not limited to, 50°C, 55°C, 60°C, 65°C, 70°C, 75°C, and 80°C; the stirring speeds include, but are not limited to, 30 rpm, 50 rpm, 80 rpm, 100 rpm, 120 rpm, 150 rpm, 180 rpm, 200 rpm, 220 rpm, and 250 rpm; and the time includes, but is not limited to, 10 min, 20 min, 30 min, and 40 min.

[0056] It is understood that the inactivation treatment can adopt existing methods in the art that can inactivate the bacteria, such as high temperature inactivation.

[0057] In addition, it is understandable that in order to maintain stable performance, the fermented oil may also be added with an appropriate amount of preservatives, such as phenoxyethanol, and the added amount may be 0.1% to 1% by volume of the total volume of the fermented oil.

[0058] Other examples of the present application provide the use of the composition with skin firming effect as described above or the fermented oil described in the second aspect in the preparation of cosmetics with skin firming effect.

[0059] Other examples of the present application provide a cosmetic comprising the composition having skin firming efficacy as described above or the fermented oil as described above.

[0060] In some examples, the cosmetic comprises a cosmetic base and the fermented oil, wherein the fermented oil is present in the cosmetic at a mass percentage of 1% to 10%. Without limitation, the mass percentage of the fermented oil in the cosmetic includes, but is not limited to, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, and 10%.

[0061] It can be understood that the cosmetic base can be selected according to the type of cosmetic.

[0062] In some examples, the cosmetic base may include one or more of water, a humectant, a chelating agent, a thickener, a skin conditioning agent, an emulsifier, and a surfactant.

[0063] In some examples, the cosmetic base includes the following components, calculated as percentage by mass in the cosmetic:

[0064]

[0065] Furthermore, the moisturizing agent includes one or more of allantoin, butylene glycol, 1,2-hexanediol, glycerol, arginine and sodium hyaluronate.

[0066] Furthermore, the chelating agent includes disodium EDTA.

[0067] Furthermore, the thickener includes carbomer.

[0068] Furthermore, the skin conditioning agent includes hydroxyethyl cellulose.

[0069] Furthermore, the surfactant includes hydroxylated lecithin.

[0070] In some examples, the cosmetic base includes the following components, calculated as percentage by mass in the cosmetic:

[0071]

[0072] The following are specific examples. Unless otherwise specified, the raw materials used in the examples are all commercially available products.

[0073] The fermentation product of Candida albicans / glucose / rapeseed oleic acid methyl ester was purchased from Hangzhou Oushi Technology Co., Ltd.

[0074] Example 1

[0075] This embodiment is a fermented oil with skin tightening effect, and its preparation method is as follows:

[0076] (1) Inoculate 4% by mass of Pichia pastoris (purchased from Shanghai Xinyu Biotechnology Co., Ltd.) into MRS broth medium (purchased from Guangdong Huankai Biotechnology Co., Ltd.) and ferment at 37°C for 12 h. Then, filter the fermentation product and collect the filtrate to obtain a culture medium fermentation broth containing the strain;

[0077] (2) Soy isoflavones, β-nicotinamide mononucleotide, and Candida albicans / glucose / rapeseed oleic acid methyl ester fermentation product were mixed in a mass ratio of 1:1:1 under the following mixing conditions: temperature 60°C, stirring speed 150 rpm, and time 30 min to obtain an oil phase;

[0078] (3) mixing the oil phase of step (2), the culture medium fermentation broth containing the bacterial strain of step (1), and water in a mass ratio of 1 g:0.5 g:5 g, and stirring and fermenting the resulting mixture again under the following conditions: fermentation temperature of 40° C., stirring speed of 200 rpm, and fermentation time of 100 h to obtain the fermentation product;

[0079] (4) The fermentation product was boiled for 5 minutes and then centrifuged to remove the microorganisms. 1% by volume of phenoxyethanol was added to the centrifuge liquid, and the resulting mixed oil was filtered through 200 mesh to obtain the fermented oil.

[0080] Example 2

[0081] This embodiment is a fermented oil with skin firming effect. The main difference between its preparation method and that of Example 1 is that the conditions for stirring and fermenting again in step (3) are: fermentation temperature 50°C, stirring speed 250 rpm, and fermentation time 80 h.

[0082] Example 3

[0083] This embodiment is a fermented oil with skin firming effect. The main difference between its preparation method and that of Example 1 is that the conditions for stirring and fermenting again in step (3) are: fermentation temperature 30°C, stirring speed 150 rpm, and fermentation time 120 h.

[0084] Comparative Examples 1 to 6

[0085] The fermented oils with skin-tightening properties provided in Comparative Examples 1 to 6 are prepared by methods similar to those in Example 1, primarily in that one or two of the soybean isoflavones, β-nicotinamide mononucleotide, and Candida albicans / glucose / rapeseed methyl ester fermentation products used in step (2) are omitted. For details, see Table 1 below (mass ratios):

[0086] Table 1

[0087]

[0088]

[0089] Test example:

[0090] In the efficacy verification of the active ingredient (fermented oil of the present application), taking into account the skin feel and other effects (such as moisturizing, etc.) of the subjects, the active ingredient is generally prepared into a cosmetic in the form of an essence and then applied to the skin of the subject. The efficacy of the cosmetic can prove the efficacy of the active ingredient.

[0091] The fermented oils prepared in the examples and comparative examples were made into essences, and the formulas (mass percentages) were shown in Tables 2-3 below:

[0092] Table 2

[0093]

[0094] Table 3

[0095]

[0096]

[0097] The preparation method of the above essence is as follows:

[0098] (1) Carbomer, sodium hyaluronate, hydroxyethyl cellulose, disodium EDTA, allantoin, glycerin, fermented oil and hydroxylated lecithin are mixed and stirred evenly;

[0099] (2) adding purified water to the mixture obtained in step (1) and stirring uniformly;

[0100] (3) Add arginine, 1,2-hexanediol and butanediol to the mixture obtained in step (2), stir evenly, and discharge.

[0101] The research subjects were 130 Chinese women with sensitive skin, aged between 25 and 45 years old, with an average age of 38.59±2.49 years old. There were 10 subjects in each group, and they used the essence of each application example and the comparative application example respectively. The subjects gave informed written consent. The subjects were willing to cooperate and understand the necessity and duration of the control in order to comply with the plan established by the clinical trial center. The test samples (essence of the application example and the comparative application example) were applied to the cheeks once a day in the morning and evening for 28 consecutive days. The experimenter used a skin elasticity meter on D0, D14 and D28 respectively. The dual MPA580 was used to assess skin firmness. Data were analyzed using SPSS 28.0. Normal distribution was tested. If the data were normally distributed, a T-test was used for statistical analysis. If the data were non-normally distributed, a rank-sum test was used for statistical analysis. Rank-sum data were analyzed using a rank-sum test. Statistical significance was set at p < 0.05.

[0102] Skin firmness: using a skin elasticity meter The dual MPA580 monitor measures skin firmness. A decrease in the skin firmness F4 measurement indicates improved skin firmness.

[0103] Skin elasticity: using a skin elasticity tester Dual MPA580s tested skin elasticity. Increased values ​​for R2, R5, and R7 indicate improved skin elasticity.

[0104] The test results of application example 1 are as follows Figures 1 to 4 shown.

[0105] Depend on Figure 1 It can be seen that after using the sample for 14 days, the skin firmness F4 value of the test area of ​​the subject decreased significantly by 9.82%. After using the sample for 28 days, the skin firmness F4 value of the test area of ​​the subject decreased significantly by 19.64%.

[0106] Depend on Figures 2-4 It can be seen that after using the sample for 14 days, the skin elasticity R2, R5 and R7 values ​​of the test area of ​​the subjects increased by 7.39%, 10.45% and 9.73% respectively. After using the sample for 28 days, the skin elasticity R2, R5 and R7 values ​​of the test area of ​​the subjects increased by 11.85%, 11.44% and 12.17% respectively.

[0107] In summary, compared to before sample use, after 14 and 28 days of sample use, the subjects' cheek skin firmness F4 value decreased significantly, while skin elasticity R2, R5, and R7 increased significantly. This indicates that the sample can effectively improve skin firmness and elasticity, and has a good firming effect.

[0108] The test results of the remaining application examples and comparative application examples after 28 days of use are summarized in Table 4 below (the data in the table are the average values ​​of the subjects in each group).

[0109] Table 4

[0110] Skin firmness F4 change rate Skin elasticity R2 change rate Skin elasticity R5 change rate Skin elasticity R7 change rate Application Example 1 -19.64％ +11.85％ +11.44％ +12.17％ Application Example 2 -15.59％ +8.45％ +8.25％ +10.35％ Application Example 3 -12.25％ +5.51％ +5.62％ +8.36％ Application Example 4 -21.92％ +14.28％ +13.59％ +15.26％ Application Example 5 -15.05％ +8.13％ +8.62％ +10.68％ Application Example 6 -14.26％ +7.93％ +8.09％ +10.18％ Comparative Application Example 1 -14.05％ +7.35％ +7.86％ +9.95％ Comparative Application Example 2 -13.86％ +7.09％ +7.21％ +9.81％ Comparative Application Example 3 -13.93％ +7.12％ +7.25％ +9.92％ Comparative Application Example 4 -10.26％ +5.15％ +5.32％ +6.95％ Comparative Application Example 5 -10.52％ +5.26％ +5.61％ +7.05％ Comparative Application Example 6 -10.39％ +5.21％ +5.36％ +6.97％ Comparative Application Example 7 -5.59％ +1.25％ +1.35％ +1.55％

[0111] The technical features of the above-mentioned embodiments can be combined arbitrarily. In order to make the description concise, not all possible combinations of the technical features in the above-mentioned embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0112] The embodiments described above only express several implementation methods of the present application, which are convenient for understanding the technical solutions of the present application in a specific and detailed manner, but they cannot be understood as limiting the scope of protection of the patent application. It should be pointed out that for ordinary technicians in this field, without departing from the concept of the present application, several variations and improvements can be made, which all fall within the scope of protection of the present application. It should be understood that the technical solutions obtained by those skilled in the art through logical analysis, reasoning or limited experiments on the basis of the technical solutions provided in the present application are all within the scope of protection of the claims attached to the present application. Therefore, the scope of protection of the patent application of this application shall be based on the content of the attached claims, and the description and drawings can be used to interpret the content of the claims.

Claims

1. A fermented oil, characterized in that Prepared by mixed fermentation of a composition with skin firming effect; The composition comprises soy isoflavones, β-nicotinamide mononucleotide and a fermentation product of Candida albicans / glucose / rapeseed oleic acid methyl ester; the mass ratio of the soy isoflavones, β-nicotinamide mononucleotide and the fermentation product of Candida albicans / glucose / rapeseed oleic acid methyl ester is (0.8-1.2):(0.8-1.2):(0.8-1.2); The Candida albicans / glucose / rapeseed oleic acid methyl ester fermentation product was purchased from Hangzhou Oushi Technology Co., Ltd. The method for preparing the fermented oil comprises the following steps: Mixing the components of the composition having skin tightening effect to prepare an oil phase; The oil phase is mixed with a culture medium fermentation liquid containing the bacterial strain and water to carry out a fermentation process; performing an inactivation treatment on the material obtained from the fermentation treatment to prepare the fermentation oil; The bacterial strain used in the bacterial strain-containing culture medium fermentation liquid is Pichia pastoris.

2. The fermented oil according to claim 1, characterized in that In the composition, the mass ratio of the soy isoflavones, β-nicotinamide mononucleotide and the fermentation product of Candida albiflora / glucose / rapeseed methyl oleate is 1:1:

1.

3. The fermented oil according to claim 1, characterized in that The culture medium used in the culture medium fermentation liquid containing the bacterial strain is MRS broth culture medium.

4. The fermented oil according to any one of claims 1 to 3, characterized in that The mass ratio of the oil phase to the culture medium fermentation liquid containing the bacterial strain and water is (0.1~2):(0.1~1):(1~10).

5. The fermented oil according to any one of claims 1 to 3, characterized in that The fermentation conditions include: a temperature of 30°C to 50°C, a stirring speed of 150 rpm to 250 rpm, and a time of 80 h to 120 h; and / or, The mixing conditions of the components include: temperature of 50° C. to 80° C., stirring speed of 30 rpm to 250 rpm, and time of 10 min to 40 min.

6. Use of the fermented oil according to any one of claims 1 to 5 in the preparation of cosmetics having skin firming effect.

7. A cosmetic, characterized in that: The invention relates to a fermented oil comprising the fermented oil according to any one of claims 1 to 5.

8. The cosmetic according to claim 7, characterized in that The cosmetic comprises a cosmetic base and the fermented oil, wherein the mass percentage of the fermented oil in the cosmetic is 1% to 10%.

Images