Device and method for measuring the avoidance of warehouse insects

Abstract

The present invention provides a device and method for measuring the repulsiveness of grain insects. The device comprises a test chamber for housing test insects; an odor source release unit disposed on one side of the test chamber and connected to the test chamber; an odor guide unit disposed on a side of the test chamber opposite the odor source release unit and connected to the test chamber; the odor guide unit comprises a negative pressure air pump and an airflow guide, connected to the airflow guide; a first end of the airflow guide has multiple guide holes, connected to the test chamber via the multiple guide holes; and an observation unit disposed at the top of the test chamber. The device ensures unidirectional and balanced gas flow within the test chamber through the odor source release unit and the odor guide unit, forming a parallel, unilaterally moving odor field. This facilitates observation of the test insect's repulsive behavior and simulates the attraction or repellency of odorants in a grain storage environment on the test insects.

Description

Technical Field

[0001] The present application relates to the technical field of warehouse beetle avoidance, and in particular to a device for measuring warehouse beetle avoidance and a method for measuring warehouse beetle avoidance. Background Art

[0002] In integrated pest management, many artificially synthesized semiochemicals have been applied as new, eco-friendly pest control technologies. This biomimetic approach offers advantages such as cost-effectiveness, safety, effectiveness, harmlessness, energy conservation, protection of natural enemies, and environmental friendliness. Using plant-based materials to attract and capture pests in stored-grain pest control can control pest populations, monitor population dynamics, and provide forecasts. GB / T41278, "Guidance for the Trapping and Detection of Stored Pests in Cereals and Legumes," describes the use of food-based materials such as cereals and legumes to attract pests.

[0003] Existing methods for testing pest attraction and avoidance often involve placing a sheet of filter paper of the same diameter at the bottom of a 9cm petri dish, cutting it in half along the midline. One half is then loaded with samples of varying concentrations, while the other half is loaded with an equal amount of solvent as a blank control. After the solvent has evaporated naturally, the treated filter paper is immediately glued to the bottom of the dish with glue, ensuring that no gaps remain to prevent the test insects from burrowing under the filter paper and obstructing observation. A predetermined number of test insects are dropped from the center of the dish, and the dish lid is quickly closed to seal. After a certain period of time, the number of test insects that settle on the filter paper in the treated and control groups is recorded, and the avoidance rate is calculated. Some laboratories also use a Y-shaped olfactometer to measure pest attraction. The Y-shaped olfactometer consists of a centrifugal air pump, a Y-shaped glass tube, a material delivery bottle, an activated carbon air filter, an air humidifier, and an airflow meter, all connected in sequence with rubber tubing. Several test insects are selected and placed at the test insect delivery point on the adaptation arm of the Y-shaped tube. Food is placed in the material delivery bottle connected to the treatment arm of the Y-shaped tube, while the material delivery bottle connected to the control arm is left empty. Air is ventilated at a constant flow rate for a set period of time, allowing the test insects to make their selection. Both material-dispensing bottles were left empty for testing as a control group. Each experiment was run in parallel, with the treated and control arms swapped. The number of test insects in the acclimated, treated, and control arms was observed, and the insect attraction and repulsion rates, as well as the insects' food selection coefficients, were calculated. Other insect selection devices utilize a larger flat platform with various material placement points positioned around it. The center point of the surrounding area serves as the test insect release point. After stabilizing the environment, test insects are released at this point, and the insects' preference for the surrounding materials is observed and compared. Furthermore, devices based on the "Y" olfactometer have been developed to measure insect selectivity for multiple odor sources, such as four- and six-arm olfactometers. The central area of ​​these four- and six-arm olfactometers serves as the insect release zone, surrounded by three or five treatment zones and one control zone, evenly distributed around them. During the experiment, the treatment and control zones are interlaced, and a movement channel is provided between the central release zone and the treatment zone. Activated carbon, a humidifier, and an airflow meter are connected from far to near. The central insect release area has an opening at the top connected to a vacuum pump. Before testing, test insects with consistent physiological indicators are placed into the central activity chamber through the air outlet. When the vacuum pump is turned on, the test insects in the central activity chamber encounter the turbulent flow of gas containing the signaling substance and then move along the odor concentration gradient. The number of insects is then recorded.

[0004] However, there is no experimental method or device in the prior art that sets the odor source to guide the odor field into parallel directional movement, places the test insects on the same starting line in the middle of the odor field, and observes their selection tendency in an environment with a concentration gradient odor field. Summary of the Invention

[0005] In view of this, in order to overcome the defects of the prior art, the present invention provides a device for measuring the avoidance of warehouse beetles and a method for measuring the avoidance of warehouse beetles, which effectively solves the problem of not setting the odor source to guide the odor field into parallel directional movement, placing the test insects on the same starting line in the middle of the odor field, and observing their selective tendency in an environment with a concentration gradient odor field.

[0006] According to a first aspect of the present invention, a device for measuring the avoidance of warehouse insects is provided, wherein the device for measuring the avoidance of warehouse insects includes a test air chamber for placing test insects and observing the test insects' tendency behavior; an odor source releasing portion is arranged on one side of the test air chamber, and the odor source releasing portion is connected to the test air chamber; an odor guiding portion is arranged on a side of the test air chamber opposite to the odor source releasing portion, and the odor guiding portion is connected to the test air chamber; the odor guiding portion includes a negative pressure air pump and an airflow guide, and the negative pressure air pump is connected to the airflow guide; a first end of the airflow guide is provided with a plurality of guide holes, and the airflow guide is connected to the test air chamber through the plurality of guide holes; and an observation portion is arranged at the top of the test air chamber.

[0007] Preferably, the odor source release part includes an odor source release chamber, an odor diffusion chamber, a first air duct and a circulating air pump. The odor diffusion chamber is arranged on one side of the test air chamber, the odor diffusion chamber is connected to the test air chamber, the circulating air pump is arranged on the first air duct, and the odor source release chamber is connected to the odor diffusion chamber through the first air duct.

[0008] Preferably, two side portions of the test air chamber communicating with the odor diffusion chamber and the airflow guide are opposite to and parallel to each other.

[0009] Preferably, a permeability barrier is provided on the side of the odor diffusion chamber communicating with the test air chamber.

[0010] Preferably, the plurality of guide holes are evenly arranged at the first end of the airflow guide, and the negative pressure air pump is connected to the airflow guide through a second air guide pipe.

[0011] Preferably, the test chamber comprises a bottom plate and a sealing cover, the bottom plate is provided with a coordinate grid, a test insect placement point is provided in the middle of the bottom plate; the sealing cover is provided at the end of the test chamber.

[0012] According to a second aspect of the present invention, a method for measuring the avoidance of warehouse insects is provided, wherein the method for measuring the avoidance of warehouse insects comprises: placing an odor substance in an odor source release portion to release the odor, and releasing the odor into a test air chamber through the odor source release portion; guiding the odor in the test air chamber through an odor guide portion so that the gas containing the odor in the test air chamber flows in parallel and one direction to the odor guide portion; placing a test insect, and placing the test insect in the test air chamber; observing the test insect's avoidance selection behavior and activity trajectory through an observation portion, and recording the avoidance selection and data.

[0013] Preferably, the odor is driven by a circulating air pump to pass through the first air duct and enter the odor diffusion chamber, and the odor entering the odor diffusion chamber is diffused into the test air chamber through a permeability barrier.

[0014] Preferably, the odor entering the test air chamber is driven by the negative pressure air pump to flow from one side of the test air chamber to the other side and enter the air flow guide.

[0015] Preferably, the test insects are placed inside a placement device, and there are multiple placement devices. The multiple placement devices are arranged in a row on the bottom plate of the test chamber, and the multiple placement devices arranged in a row are parallel to both sides of the test chamber.

[0016] According to the device for measuring the attraction and avoidance of warehouse insects of the present invention, the gas flow in the test air chamber is unidirectional and balanced through the negative pressure air pump and airflow guide of the odor source release part and the odor guide part, forming an odor field that is parallel and moves to one side. At the same time, it is convenient to observe the attraction behavior of the test insects in the concentration gradient odor field, and then measure the specific attraction or repellency of the odor substances on the test insects in the simulated grain warehouse environment.

[0017] In order to make the above-mentioned objects, features and advantages of the present application more obvious and easy to understand, preferred embodiments are given below and described in detail with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following is a brief introduction to the drawings required for use in the embodiments. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without creative work.

[0019] Figure 1 A schematic structural diagram of a device for measuring the avoidance of warehouse insects according to an embodiment of the present invention is shown.

[0020] Figure 1: 1-test air chamber; 101-bottom plate; 102-sealing cover; 2-odor source release chamber; 201-odor substance; 3-odor diffusion chamber; 301-permeability grid; 4-first air duct; 5-circulation air pump; 6-air flow guide; 601-guide hole; 7-second air duct; 8-negative pressure air pump; 9-placer; 10-test insect; 11-observation part. DETAILED DESCRIPTION

[0021] The following detailed description is provided to help the reader gain a comprehensive understanding of the methods, devices, and / or systems described herein. However, various changes, modifications, and equivalents of the methods, devices, and / or systems described herein will be apparent upon understanding the disclosure of this application. For example, the order of operations described herein is merely illustrative and is not limited to the order set forth herein. Rather, except for operations that must occur in a particular order, changes may be made that will be apparent upon understanding the disclosure of this application. Furthermore, descriptions of features known in the art may be omitted for clarity and brevity.

[0022] The features described herein may be implemented in different forms and should not be construed as being limited to the examples described herein. Rather, the examples described herein have been provided merely to illustrate some of the many possible ways to implement the methods, devices, and / or systems described herein that will be apparent upon understanding the disclosure of this application.

[0023] Throughout the specification, when an element (such as a layer, region, or substrate) is described as being “on,” “connected to,” “coupled to,” “over,” or “overlaying” another element, it may be directly “on,” “connected to,” “coupled to,” “over,” or “overlaying” another element, or one or more other elements may be present between them. In contrast, when an element is described as being “directly on,” “directly connected to,” “directly coupled to,” “directly over,” or “directly covering” another element, there may be no other elements intervening therebetween.

[0024] As used herein, the term "and / or" includes any one of the associated listed items and any combination of any two or more items.

[0025] Although terms such as "first," "second," and "third" may be used herein to describe various members, components, regions, layers, or portions, these members, components, regions, layers, or portions are not limited by these terms. Rather, these terms are used only to distinguish one member, component, region, layer, or portion from another member, component, region, layer, or portion. Thus, a first member, component, region, layer, or portion in the examples described herein may also be referred to as a second member, component, region, layer, or portion without departing from the teachings of the examples.

[0026] For ease of description, spatial relational terms such as "above," "upper," "below," and "lower" may be used herein to describe the relationship of one element to another element as shown in the accompanying drawings. Such spatial relational terms are intended to encompass different orientations of the device in use or operation in addition to the orientations depicted in the accompanying drawings. For example, if the device in the accompanying drawings is turned over, an element described as being "above" or "upper" relative to another element would subsequently be "below" or "lower" relative to the other element. Thus, the term "above" includes both the orientations "above" and "below," depending on the spatial orientation of the device. The device may also be positioned in other ways (e.g., rotated 90 degrees or in other orientations), and the spatial relational terms used herein will be interpreted accordingly.

[0027] The terms used herein are intended only to describe various examples and are not intended to limit the present disclosure. Unless the context clearly indicates otherwise, the singular is intended to include the plural. The terms "comprise," "include," and "have" list the presence of stated features, quantities, operations, components, elements, and / or combinations thereof, but do not preclude the presence or addition of one or more other features, quantities, operations, components, elements, and / or combinations thereof.

[0028] Due to manufacturing techniques and / or tolerances, variations in the shapes shown in the drawings may occur. Therefore, the examples described herein are not limited to the specific shapes shown in the drawings but include changes in shapes that occur during manufacturing.

[0029] The features of the examples described herein can be combined in various ways that will be apparent after understanding the disclosure of the present application. In addition, although the examples described herein have various configurations, other configurations are possible as will be apparent after understanding the disclosure of the present application.

[0030] According to a first aspect of the present invention, there is provided a device for measuring the avoidance of warehouse insects, such as Figure 1As shown, the device for measuring insect repellency is used to simulate a stored grain environment where grain, for example, emits volatile substances that attract pests. The device then detects the attraction (attraction) or repellency (insect control) effect of a target odorant 201 on a test insect 10. Odorants 201 can include, for example, peanut volatiles, n-heptadecane, or decane. Test insects 10 can include, for example, adults and larvae of the red flour beetle or larvae of the Indian meal borer. The device includes a test chamber 1, an odor source release unit, an odor guide unit, and an observation unit 11.

[0031] In the following description, reference will be made to Figure 1 The detailed structures of the test air chamber 1, the odor source release part, the odor guide part and the observation part 11 of the device for measuring the avoidance of warehouse insects are specifically described.

[0032] like Figure 1 As shown, in an embodiment, a test chamber 1 is used to measure the tropism of a test insect 10. The test chamber 1 can be used to simulate a granary environment, thereby detecting and observing the effect of a target odorant 201 on the test insect 10. An odor source release portion and an odor guide portion are respectively disposed on either side of the test chamber 1. The odor source release portion is used to place the odorant 201 and supply the odor of the odorant 201 to the test chamber 1. The odorant 201 that enters the test chamber 1 then flows out through the odor guide portion. During this process, the test insect 10 is placed in the test chamber 1. Since the gas containing the odorant 201 is flowing, an observer can observe the movement of the test insect 10 using the observation portion 11 disposed at the top of the test chamber 1. Specifically, in the test chamber 1, since the gas containing the odor substance 201 moves in a parallel and directional manner, that is, it is guided into an odor field with parallel and directional movement by the odor guiding part, at this time, since the gas is guided to flow, there is a concentration gradient in the odor field in the test chamber 1. The test insect 10 is placed in the middle of the test chamber 1. At this time, the selection tendency of the test insect 10 in this environment can be observed through the observation part 11.

[0033] like Figure 1 As shown, in an embodiment, the odor guiding part can be provided on a side of the test air chamber 1 relative to the odor source releasing part, and the odor guiding part is connected to the test air chamber 1. Specifically, the odor guiding part can include a negative pressure air pump 8 and an airflow guide 6, and the negative pressure air pump 8 is connected to the airflow guide 6; a first end of the airflow guide 6 (i.e., an end close to the test air chamber 1) is provided with a plurality of guide holes 601, and the airflow guide 6 is connected to the test air chamber 1 through the plurality of guide holes 601. In an embodiment, the shape and size of the airflow guide 6 can correspond to the test air chamber 1, that is, the airflow guide 6 needs to fit one side of the test air chamber 1 to facilitate the guiding of the airflow. As Figure 1As shown, in the embodiment, since the test chamber 1 is formed into a rectangular parallelepiped structure, the airflow guide 6 is also formed into a rectangular parallelepiped structure accordingly.

[0034] Preferably, if Figure 1 As shown, in an embodiment, the test air chamber 1 includes a base plate 101 and a sealing cover 102. Base plate 101 is used to carry test worm 10 and following placement device 9 as the bottom of the test air chamber 1, and by the movement of the test worm 10 on the base plate 101, the avoidance of the test worm 10 is observed. The base plate 101 can be provided with a coordinate grid (not shown), and the coordinate grid is convenient for the observer to place and observe the test worm 10, and the record of the test structure is prepared. The horizontal and vertical coordinate spacing of the coordinate grid can be specifically arranged according to the type and size of the test worm 10 and the data that need to be recorded. The middle part of the base plate 101 is also provided with a test worm placement point. Since the odor field concentration of the inside of the test air chamber 1 is a gradient setting, gas flow is unidirectional and horizontal simultaneously. The gas concentration of the test worm placement point in the middle is moderate, which is convenient for test, and is also convenient for the observer to observe the movement of the test worm 10 intuitively. A sealing cover 102 is provided at the end of the test chamber 1. The sealing cover 102 can be, for example, an end surface of the test chamber 1 formed into a rectangular parallelepiped structure. The sealing cover 102 is provided to allow the observer to place the test insect 10 and the placement device 9 described below. At the same time, in order to ensure the unidirectional and parallel flow of the gas in the test chamber 1, the overall sealing of the test chamber 1 needs to be ensured. The sealing cover 102 can be connected to the test chamber 1 in a manner such as a lock connection, a sliding connection, or a flip connection. A sealing strip can be provided around the side where the sealing cover 102 is connected to the test chamber 1.

[0035] Preferably, if Figure 1 As shown in the embodiment, the two sides of the test chamber 1 that connect the odor diffusion chamber 3 and the air flow guide 6 are parallel. The air inlet end and the air outlet end of the test chamber 1 need to be parallel to meet the parallelism requirement of the gas flow. Figure 1 As shown, the test chamber 1 can be formed into a rectangular parallelepiped structure, and the two sides of the test chamber 1 connecting the odor diffusion chamber 3 and the air flow guide 6 are Figure 1 The left and right ends of .

[0036] Preferably, if Figure 1 As shown in the embodiment, the odor source release part includes an odor source release chamber 2, an odor diffusion chamber 3, a first air guide pipe 4 and a circulating air pump 5. Specifically, the odor source release chamber 2 is arranged on one side of the test air chamber 1 (such as Figure 1The odor source release chamber 2 needs to be connected to the test air chamber 1. The circulating air pump 5 is provided on the first air duct 4. The odor source release chamber 2 is connected to the odor diffusion chamber 3 through the first air duct 4. The odor source release chamber 2 is used to place the odor substance 201. The circulating air pump 5 is provided on the first air duct 4 so that the odor of the odor substance 201 circulates from the odor source release chamber 2 to the odor diffusion chamber 3 ( Figure 1 The arrows shown in the figure indicate the direction of gas flow. The odor source release chamber 2 can be formed into a cylindrical barrel structure, with two first air ducts 4 connected to either side of the barrel structure. The first first air duct 4 is connected to the air inlet of the odor diffusion chamber 3, and the second first air duct 4 is connected to the air outlet of the odor diffusion chamber 3. The gas containing the odor substance 201 is driven by a circulating air pump 5 to flow within the odor source release portion. The circulating air pump 5 can adjust the flow rate. In this embodiment, the operating rate of the circulating air pump 5, i.e., the flow rate of the gas, can be relatively slow, resulting in the distribution and slow supply of the odor substance 201, thereby simulating the odor emission in a granary.

[0037] like Figure 1 As shown, in the embodiment, the shape and size of the odor diffusion chamber 3 can correspond to the test chamber 1, that is, the odor diffusion chamber 3 needs to fit one side of the test chamber 1 to facilitate the guidance of the airflow. Figure 1 As shown, in the embodiment, since the test air chamber 1 is formed into a rectangular parallelepiped structure, the odor diffusion chamber 3 is also formed into a rectangular parallelepiped structure accordingly.

[0038] Preferably, if Figure 1 As shown, in the embodiment, a permeability barrier 301 is provided on the side of the odor diffusion chamber 3 that is connected to the test gas chamber 1. The permeability barrier 301 can serve as the side of the odor diffusion chamber 3 that is connected to the test gas chamber 1. The permeability barrier 301 can be, for example, an acrylic plate with openings. The openings can be adjusted to adjust the air permeability. When the gas containing the odor substance 201 circulates in the odor source release part, part of the gas passes through the permeability barrier 301 and enters the test gas chamber 1.

[0039] Preferably, if Figure 1 As shown, in this embodiment, multiple guide holes 601 are evenly distributed at the first end of the airflow guide 6, and the negative pressure air pump 8 is connected to the airflow guide 6 via the second air guide tube 7. The negative pressure air pump 8 negatively pumps the gas within the test chamber 1 through the airflow guide 6 via the second air guide tube 7. Because the airflow guide 6 has multiple evenly distributed guide holes 601, the gas flow during negative pressure extraction is balanced and flows out to the airflow guide 6 in a single, parallel line. In addition, during negative pressure extraction, the operating rate of the negative pressure air pump 8 can be consistent with that of the circulating air pump 5, which facilitates the stability of the gas flow.

[0040] Preferably, if Figure 1As shown, in the embodiment, the test insects 10 can be, for example, storage pests such as adults and larvae of red flour beetles, larvae of Indian meal borer, etc., and the number of test insects 10 is multiple, and the multiple test insects 10 are arranged in a row in the middle of the bottom plate 101 of the test chamber 1. The test insects 10 arranged in a row are parallel to the left and right sides of the test chamber 1 and perpendicular to the flowing gas, which is convenient for testing.

[0041] Preferably, if Figure 1 As shown, in the embodiment, the observation part 11 is arranged on the top of the test air chamber 1. The observation part 11 can be, for example, a camera and connected to an external display. The observation range of the observation part 11 can be, for example, as shown by the dotted line in the figure. It is necessary to ensure that the left and right observations of the test air chamber 1 can be fully observed. In order to avoid interference from the observer's own odor, the device for measuring the avoidance of warehouse insects can be placed in a specific observation room, and the observer can observe through an external display.

[0042] In addition, it should be noted that the above-mentioned test air chamber 1, odor source release chamber 2, odor diffusion chamber 3, airflow guide 6 and the following placement device 9 can all be made of acrylic plates, which are transparent, thin and easy to observe.

[0043] The device for measuring the attraction and avoidance of warehouse insects uses a negative pressure air pump and an airflow guide in the odor source release part and the odor guide part to make the gas flow in the test air chamber unidirectional and balanced, forming an odor field that is parallel and moves to one side. At the same time, it is convenient to observe the attraction behavior of test insects in the concentration gradient odor field, and then measure the specific attraction or repellency of odor substances on test insects in a simulated grain warehouse environment.

[0044] Furthermore, according to a second aspect of the present invention, there is provided a method for determining the repellency of warehouse insects, the method comprising:

[0045] In the first step, the odor material 201 is placed in the odor source release part to release the odor, and the odor is released into the test air chamber 1 through the odor source release part;

[0046] In the second step, the odor in the test chamber 1 is guided by the odor guide, so that the odor-containing gas in the test chamber 1 flows in parallel and in one direction to the odor guide;

[0047] The third step is to place the test insect 10 in the test air chamber 1;

[0048] The fourth step is to observe the approach and avoidance choices and activity tracks of the test insects 10 through the observation unit 11, and record the approach and avoidance choices and data.

[0049] Preferably, in the embodiment, in the first step, the gas containing odor is driven by the circulating air pump 5 through the first air duct 4 into the odor diffusion chamber 3, and the odor entering the odor diffusion chamber 3 is diffused into the test air chamber 1 through the permeability grid 301.

[0050] Preferably, in the embodiment, in the second step, the odor entering the test air chamber 1 flows from one side of the test air chamber 1 to the other side and enters the air flow guide 6 driven by the negative pressure air pump 8, thereby realizing unidirectional and parallel flow of the gas containing the odor.

[0051] Preferably, in the embodiment, in the third step, the test insect 10 can be placed inside the placer 9, the number of the placer 9 is multiple, and a test insect 10 is placed in each placer 9, and the multiple placers 9 are arranged in a row on the bottom plate 101 of the test chamber 1, and the multiple placers 9 arranged in a row are parallel to the two sides of the test chamber 1.

[0052] The method for determining the attraction and avoidance of warehouse insects is to set up an odor field that guides parallel directional movement with odors, and place test insects on the same starting line in a test air chamber. By observing the tendency of the test insects to choose in an environment with a concentration gradient odor field, observing the test insects' forward or reverse movement relative to the odor direction, or random movement trends unaffected by the odor, and recording and comparing the results, the method can detect the effect of odor substances on the attraction (attraction) or repellency (insect prevention) of the test insects while simulating a grain storage scene.

[0053] The method for measuring the avoidance of warehouse insects uses a device for measuring the avoidance of warehouse insects that can be 60 cm long, 50 cm wide, and 10 cm high. The overall size and volume of the device for measuring the avoidance of warehouse insects are large in order to better test the authenticity of insect movements, avoiding the problem of reduced authenticity of insect movements caused by the small volume of the device in existing insect research.

[0054] The experimental results obtained by using this method for measuring the avoidance of warehouse insects are shown below.

[0055] The odor source release chamber 2 of the apparatus for measuring insect attraction and avoidance was equipped with a blank sample and two control groups containing decanal at concentrations of 10, 50, or 100 μg / μL. Gas was introduced into the test chamber 1 using the three airflow rates listed in Table 1 below. Thirty seconds after the introduction of a test insect 10, for example, an Indian meal borer larva, the test insect 10 moved halfway. One minute later, the number of test insects in both the positive and negative directions is shown in Table 1.

[0056] The main results are: in the blank control, there are 10 test insects on both sides and they are randomly distributed.

[0057] When the flow rate was 1 L / min, the Indian meal moth crawled to both sides of the insect chamber, indicating that decanal had no significant effect on it.

[0058] When the concentration was 1 μg / μL, the flow rate was 0.2 L / min and 1 L / min; when the concentration was 50 μg / μL, the flow rate was 1 L / min, decanal had an evasive effect on Indian meal moth, and the effect was most obvious when the concentration was 1 μg / μL and the flow rate was 0.2 L / min.

[0059] Under other conditions, decanal attracted Indian meal moth, and the effect was most significant when the concentration was 10 μg / μL and the flow rate was 1 L / min.

[0060] Preliminary tests have shown that the device can effectively measure the directional behavior of the test insect 10. Later, observations at different locations and times can be added for better refinement and comparison.

[0061]

[0062] (Table 1: The odor source is decanal. The trend and position of Indian meal moth in the airflow field at different airflow velocities at three decanal concentrations)

[0063] Note: 1) - means the test insect 10 moves toward the odor diffusion chamber 3, + means the test insect moves toward the airflow guide 6;

[0064] 2) Mean ± standard error, i.e., statistical value of 3 parallel experiments;

[0065] 3) The total number of test insects 10 used in the tests in the table is different. The sum of both sides equals the total number used.

[0066] Finally, it should be noted that the above-described embodiments are only specific implementation methods of the present application, which are used to illustrate the technical solutions of the present application, rather than to limit them. The scope of protection of the present application is not limited thereto. Although the present application has been described in detail with reference to the above-described embodiments, those skilled in the art should understand that any person skilled in the art can modify or easily conceive of changes to the technical solutions described in the above-described embodiments within the technical scope disclosed in the present application, or perform equivalent replacements for some of the technical features thereof. These modifications, changes, or replacements do not deviate from the spirit and scope of the technical solutions of the embodiments of the present application, and should be included in the scope of protection of the present application. Therefore, the scope of protection of the present application shall be subject to the scope of protection of the claims.

Claims

1. A device for measuring the avoidance of warehouse insects, characterized in that: The device for measuring the avoidance of warehouse insects comprises: Test air chamber, used to place test insects and observe their directional behavior; an odor source releasing portion, disposed on one side of the test air chamber, the odor source releasing portion being in communication with the test air chamber; An odor guide is provided on a side of the test air chamber opposite to the odor source release portion, the odor guide being connected to the test air chamber; the odor guide comprises a negative pressure air pump and an air flow guide, the negative pressure air pump being connected to the air flow guide; a first end of the air flow guide is provided with a plurality of guide holes, the air flow guide being connected to the test air chamber through the plurality of guide holes; An observation portion, disposed on the top of the test chamber; The odor source release unit includes an odor source release chamber, an odor diffusion chamber, a first air duct, and a circulating air pump. The odor diffusion chamber is arranged on one side of the test air chamber and is connected to the test air chamber. The circulating air pump is arranged in the first air duct. The odor source release chamber is connected to the odor diffusion chamber through the first air duct. Two side portions of the test air chamber communicating with the odor diffusion chamber and the airflow guide are opposite to and parallel to each other.

2. The device for measuring the avoidance of warehouse insects according to claim 1, characterized in that A permeability barrier is provided on the side of the odor diffusion chamber that communicates with the test air chamber.

3. The device for measuring the avoidance of warehouse insects according to claim 2, characterized in that The plurality of guide holes are evenly arranged at the first end of the airflow guide, and the negative pressure air pump is connected to the airflow guide through a second air guide pipe.

4. The device for measuring the avoidance of warehouse insects according to claim 3, characterized in that The test chamber includes a base plate and a sealing cover, wherein the base plate is provided with a coordinate grid, and a test insect placement point is provided in the middle of the base plate; The sealing cover is arranged at the end of the test air chamber.

5. A method for determining the avoidance of warehouse insects, characterized in that: Utilizing the device for measuring the avoidance of warehouse insects according to claim 4, the method for measuring the avoidance of warehouse insects comprises: placing an odor substance in an odor source releasing portion to release the odor, and releasing the odor into the test air chamber through the odor source releasing portion; guiding the odor in the test air chamber through the odor guiding portion so that the gas containing the odor in the test air chamber flows in parallel and in one direction to the odor guiding portion; Placing the test insects in the test air chamber; The observation part is used to observe the approach-avoidance selection behavior and activity trajectory of the test insects, and the approach-avoidance selection and data are recorded.

6. The method for measuring the avoidance of warehouse insects according to claim 5, characterized in that The odor is driven by the circulating air pump and enters the odor diffusion chamber through the first air guide pipe. The odor entering the odor diffusion chamber is diffused into the test air chamber through the permeability barrier.

7. The method for measuring the avoidance of warehouse insects according to claim 6, characterized in that The odor entering the test air chamber is driven by the negative pressure air pump to flow from one side of the test air chamber to the other side and enter the air flow guide.

8. The method for measuring the avoidance of warehouse insects according to claim 7, characterized in that The test insects are placed inside a placement device. There are multiple placement devices, which are arranged in a row on the bottom plate of the test chamber. The multiple placement devices arranged in a row are parallel to both sides of the test chamber.

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