An apparatus and method for measuring the biological inhibition of water samples

Abstract

This application discloses a device for measuring the bioinhibition of water samples, including an upflow reactor, an optical observation dish, a camera, a data processing unit, and an LED light source. This application also discloses a method for measuring the bioinhibition of water samples using the above-mentioned device, comprising the following steps: S1, inoculating PN / A granular sludge into the upflow reactor, introducing water, and turning on the aeration device; S2, measuring the sludge activity q every 5 days, and discharging 4-6 ml of PN / A granular sludge into the optical observation dish; S3, taking photos of the PN / A granular sludge with the camera, and the data processing unit performing identification and statistical analysis on the photos to obtain the overall projected area A of the PN / A granular sludge photos. G The projected area A of the brick-red region in the PN / A granular sludge photograph R S4. Calculate the overall inhibition rate P according to the formula, where q is the activity of granular sludge in the wastewater, and q0 is the activity of granular sludge in the control group. This application can accurately evaluate the biological inhibition of water samples, and has a simple structure and strong operability.

Description

Technical Field

[0001] This application relates to the field of environmental protection technology, and in particular to a device and method for measuring the bioinhibition of water samples. Background Technology

[0002] Traditional water quality safety assessments rely solely on physicochemical indicators of water bodies to determine whether water quality meets standards. These indicators can only measure the content of pollutants, but cannot directly and accurately reflect their comprehensive impact on the environment. Therefore, bioinhibition monitoring is needed to supplement this.

[0003] Bioinhibition monitoring, as a supplement to traditional physicochemical testing, can directly reflect the combined toxic effects of coexisting pollutants in water bodies. It can also effectively detect the comprehensive biotoxic effects of various pollutants in water bodies and provide a clear and intuitive assessment of water quality safety. Test organisms for bioinhibition include luminescent bacteria, algae, and activated sludge. Commonly used evaluation indicators for bioinhibition include: oxygen consumption rate, adenosine triphosphate (ATP) content, and nitrification inhibition rate.

[0004] Bioluminescent bacteria are a type of bacteria that emit blue-green visible light with wavelengths of 450–490 nm under normal physiological conditions. When exposed to toxic or harmful substances in wastewater, the bacteria themselves, or their respiration or physiological processes, are inhibited, thus reducing the light intensity or even stopping the emission. Using the luminescence intensity of bioluminescent bacteria as an indicator to monitor toxic substances is time-efficient and highly sensitive; however, the reproducibility of the results using the bioluminescent bacteria method is not ideal, and the cultivation costs are high.

[0005] Algae, as primary producers in the food chain, have gained attention and widespread application due to their small size, rapid reproduction, sensitivity to toxic substances, and the ability to quickly assess the impact of chemical substances on population levels. Commonly used testing indicators include optical density, cell number, chlorophyll concentration, and cell dry weight. Among these, cell number and optical density are the most widely used due to their ease of operation and are the most important testing indicators in algal toxicity experiments. However, as prokaryotes, luminescent bacteria and algae cannot realistically simulate the complex metabolic processes of eukaryotic organisms in response to toxic substances, presenting significant limitations.

[0006] Activated sludge, as the test organism, consists of bacteria, fungi, protozoa, and metazoa, and has a complex composition. Different types of activated sludge have different compositions, which leads to unstable experimental results.

[0007] Oxygen consumption rate (OUR) refers to the rate at which microorganisms in sludge consume oxygen during pollutant degradation, and is a theoretical indicator characterizing the activity of aerobic microorganisms. OUR often uses activated sludge as the test organism; however, the complexity of activated sludge composition can lead to unstable experimental results.

[0008] Adenosine triphosphate (ATP) is a specific high-energy compound in living organisms, serving as the energy conversion substance for cells and the basis of life activities. Under specific environmental conditions, the content of ATP in an organism is relatively stable, but it is rapidly decomposed upon the death of the organism. Therefore, ATP content can serve as an indicator of biological activity, characterizing the living biomass and metabolic rate of microorganisms. However, ATP measurement is cumbersome and requires specialized equipment, limiting its practicality.

[0009] Nitrification inhibition rate refers to the nitrification process of nitrifying bacteria, which refers to the process by which nitrifying bacteria, under aerobic conditions, convert NH4+ into nitrogenous oxygen. + -N oxidation produces NH2OH and NO2. - -N, then oxidized to produce NO3 - Numerous studies have shown that nitrifying bacteria are more susceptible to selective inhibition by toxic substances in wastewater compared to other microbial communities in activated sludge. However, the complex composition of microbial communities in activated sludge leads to unstable experimental results.

[0010] Therefore, there is an urgent need for a simple and rapid method to determine the bioinhibition of water bodies. Summary of the Invention

[0011] In order to solve at least one of the above-mentioned technical problems, this application provides a device for measuring the biological inhibition of water samples and a method for measuring the biological inhibition of water samples using the device.

[0012] On the one hand, this application provides a device for measuring the biological inhibition of water samples, including an upflow reactor 1, an optical observation dish 2, a camera 3, a data processing unit, and an LED light source 4;

[0013] The upflow reactor 1 has an inlet pipe 5 at the bottom of one side and a sludge discharge pipe 6 at the bottom of the other side. The sludge discharge pipe 6 is equipped with a sampling valve 7. The inlet pipe 5 is connected to the sampling system 8. The inlet pipe 5 is equipped with a water pump 9. The bottom of the upflow reactor 1 is equipped with an aeration device 10.

[0014] The top of the optical observation dish 2 is provided with a sample inlet 11, which is connected to the outlet end of the mud discharge pipe 6. The bottom of the optical observation dish 2 is made of a light-transmitting material, and the lower side wall of the optical observation dish 2 is recessed inward to form a mounting groove 12.

[0015] The LED light source 4 is installed in the mounting slot 12;

[0016] The camera 3 is positioned below the optical observation dish 2, with its camera lens facing the bottom of the optical observation dish 2, and the camera 3 is connected to the data processing unit.

[0017] By adopting the above technical solution, this application can quantitatively determine the biological inhibition of water samples, and has a simple structure, strong operability, and can accurately evaluate the changes in comprehensive dynamic activity and morphological characteristics.

[0018] Optionally, the upflow reactor 1 is equipped with a DO monitoring probe 13, a nitrate monitoring probe 14, a nitrite monitoring probe 15, and an ammonia nitrogen monitoring probe 16, which are respectively connected to the online water quality monitoring system 17.

[0019] By adopting the above technical solution, the DO monitoring probe 13 is used to detect the DO content in the system, the nitrate monitoring probe 14 is used to detect the nitrate nitrogen content in the system, the nitrite monitoring probe 15 is used to detect the nitrite nitrogen content in the system, and the ammonia nitrogen monitoring probe 16 is used to detect the ammonia nitrogen content in the system.

[0020] Optionally, the bottom of the optical observation dish 2 is square, the lower surface of the top of the optical observation dish 2 is a backlight surface, and the sidewalls and / or bottom of the optical observation dish 2 are made of quartz glass.

[0021] By adopting the above technical solution, the sidewalls and / or bottom of the optical observation dish 2 are made of quartz glass. Quartz glass has excellent optical properties and can obtain clear photographs of PN / A particle sludge.

[0022] Optionally, the length of the LED light source 4 is 3 / 4 to 4 / 5 of the bottom length of the optical observation dish 2.

[0023] By adopting the above technical solution, the length of the LED light source 4 is 3 / 4 to 4 / 5 of the bottom length of the optical observation dish 2. The light emitted by the LED light source 4 can better cover the bottom of the optical observation dish 2. The camera 3 is located below the optical observation dish 2 and the camera is facing the bottom of the optical observation dish 2, so that the PN / A particle sludge photos taken by the camera 3 are clearer, thereby ensuring the accuracy of data processing.

[0024] Optionally, the inner wall of the mounting groove 12 is provided with an internal thread, and the outer wall of the LED light source 4 is provided with an external thread that matches the internal thread.

[0025] By adopting the above technical solution, the LED light source 4 and the mounting groove 12 are connected by threads, which makes it easy to install and remove, and the connection is tight and firm, thereby ensuring the stability of the LED light source 4.

[0026] Secondly, this application provides a method for detecting the bioinhibition of a water sample using the above-mentioned measuring device, comprising the following steps:

[0027] S1. Inoculate PN / A granular sludge into the upflow reactor 1. The PN / A granular sludge is brick red. Introduce water and turn on the aeration device 10 to control the DO concentration in the aeration tank.

[0028] S2. Based on the MLSS of the inoculated sludge, the activity q of the sludge is measured every 5 days. Every 5 days, the sampling valve 7 is opened to discharge 4~6 ml of PN / A granular sludge from the sludge discharge pipe 6 into the optical observation dish 2, so that it is evenly spread at the bottom of the optical observation dish 2.

[0029] S3. Using the camera 3, take a photograph of the PN / A granular sludge. The data processing unit identifies and statistically analyzes the vertical projected area of ​​the PN / A granular sludge displayed in the photograph to obtain the overall projected area A of the PN / A granular sludge photograph. G The projected area A of the brick-red region in the PN / A granular sludge photograph R ;

[0030] S4. Calculate the overall inhibition P according to the following formula.

[0031]

[0032] In the formula, q represents the activity of granular sludge in wastewater, and q0 represents the activity of granular sludge in the control group.

[0033] By adopting the above technical solution, this application quantitatively measures the comprehensive biological inhibition of water samples and accurately evaluates the changes in bacterial activity and particle morphology in PN / A granular sludge.

[0034] Optionally, the PN / A granular sludge has a particle size of 0.5~2mm and an MLSS of 8~15g / L.

[0035] Optionally, the microbial composition of the PN / A granular sludge includes 30-50% anaerobic ammonia oxidizing bacteria (AMX), 30-40% aerobic ammonia oxidizing bacteria (AOB), and the remainder being symbiotic heterotrophic bacteria.

[0036] By adopting the above technical solution, the microbial components of the PN / A granular sludge are mainly AMX and AOB, characterized by simple composition and distinctive appearance. Moreover, compared with traditional activated sludge which includes bacteria, fungi, protozoa, and metazoa, the microbial composition of this application is simpler, thus ensuring the reliability of the results.

[0037] Optionally, in step S1, the DO concentration in the aeration tank is controlled to be 0.6~0.8 mg / L.

[0038] Optionally, in step S5, the calculation of the overall inhibition P uses data from day 30 to evaluate biological inhibition.

[0039] By adopting the above technical solution, the activity q and A of the sludge were measured every 5 days. R / A G The values ​​were evaluated using data from day 30, because at day 30, the q-value and A... R / A G The value tends to stabilize, which can ensure the reliability of the comprehensive evaluation of biological inhibition.

[0040] In summary, the present invention has at least one of the following beneficial technical effects:

[0041] 1. This application uses an optical observation dish, a camera, and a data processing unit to determine the activity of PN / A granular sludge. Compared with existing professional testing equipment, this application has a simple structure and is highly operable.

[0042] 2. This application uses an optical observation dish to sample and photograph PN / A granular sludge, which is convenient to operate.

[0043] 3. The main microorganisms in the PN / A granular sludge used in this application are AMX and AOB. Compared with traditional activated sludge, which includes bacteria, fungi, protozoa and metazoa, the microbial composition of this application is simpler, thus ensuring the stability and reliability of the results.

[0044] 4. This application quantitatively measures the comprehensive biological inhibition of water samples and accurately evaluates the changes in the activity and morphology of the microbial community in PN / A granular sludge. Attached Figure Description

[0045] Figure 1 This is a schematic diagram of the device for measuring the biological inhibition of water samples.

[0046] Figure 2 This is a schematic diagram of the cross-sectional structure of optical observation dish 2;

[0047] Figure 3 The graph shows the effect of different municipal wastewaters on the nitrogen removal performance of PN / A granular sludge in Application Example 1.

[0048] Figure 4 To illustrate the application of different municipal wastewater treatment methods for PN / A granular sludge A in Example 1 R / A G Impact diagram;

[0049] Figure 5 The graph shows the effect of different electroplating wastewater on the denitrification performance of PN / A granular sludge in Application Example 2.

[0050] Figure 6 To illustrate the effects of different electroplating wastewaters on PN / A granular sludge A in Example 2 R / A G Impact diagram;

[0051] Explanation of reference numerals in the attached diagram: 1. Upflow reactor; 2. Optical observation dish; 3. Camera; 4. LED light source; 5. Inlet pipe; 6. Sludge discharge pipe; 7. Sampling valve; 8. Sampling system; 9. Water pump; 10. Aeration device; 11. Sampling port; 12. Mounting tank; 13. DO monitoring probe; 14. Nitrate monitoring probe; 15. Nitrite monitoring probe; 16. Ammonia nitrogen monitoring probe; 17. Online water quality monitoring system. Detailed Implementation

[0052] The present application will be further described in detail below with reference to the accompanying drawings and embodiments.

[0053] Definitions:

[0054] DO: Dissolved oxygen in molecular form in water is called dissolved oxygen, usually denoted as DO.

[0055] PN / A granular sludge: Nitrification-anaerobic ammonia oxidation granular sludge, mainly composed of anaerobic ammonia oxidizing bacteria (AMX), ammonia oxidizing bacteria (AOB), nitrifying bacteria and some filamentous heterotrophic bacteria, the granules are brick red in color.

[0056] MLSS: Mixed liquor suspended solids concentration, also known as mixed liquor sludge concentration, represents the total weight of activated sludge solids contained in a unit volume of mixed liquor in an aeration tank.

[0057] This application designs a device for measuring the biological inhibition of water samples, including an upflow reactor 1, an optical observation dish 2, a camera 3, a data processing unit, and an LED light source 4;

[0058] The upflow reactor 1 has an inlet pipe 5 at the bottom of one side and a sludge discharge pipe 6 at the bottom of the other side. The sludge discharge pipe 6 is equipped with a sampling valve 7. The inlet pipe 5 is connected to the sampling system 8. The inlet pipe 5 is equipped with a water pump 9, which is a peristaltic pump. The bottom of the upflow reactor 1 is equipped with an aeration device 10.

[0059] The top of the optical observation dish 2 is provided with a sample inlet 11, which is connected to the outlet end of the mud discharge pipe 6. The bottom of the optical observation dish 2 is made of a light-transmitting material, and the lower side wall of the optical observation dish 2 is recessed inward to form a mounting groove 12.

[0060] The LED light source 4 is installed in the mounting slot 12;

[0061] The camera 3 is positioned below the optical observation dish 2, with its camera lens facing the bottom of the optical observation dish 2, and the camera 3 is connected to the data processing unit.

[0062] This application describes a method for detecting the bioinhibition of water samples using the aforementioned measuring device, comprising the following steps:

[0063] S1. Inoculate PN / A granular sludge into the upflow reactor 1. The PN / A granular sludge is brick red. Introduce water and turn on the aeration device 10 to control the DO concentration in the aeration tank.

[0064] S2. Based on the MLSS of the inoculated sludge, the activity q of the sludge is measured every 5 days. Every 5 days, the sampling valve 7 is opened to discharge 4~6 ml of PN / A granular sludge from the sludge discharge pipe 6 into the optical observation dish 2, so that it is evenly spread at the bottom of the optical observation dish 2.

[0065] S3. Using the camera 3, take a photograph of the PN / A granular sludge. The data processing unit identifies and statistically analyzes the vertical projected area of ​​the PN / A granular sludge displayed in the photograph to obtain the overall projected area A of the PN / A granular sludge photograph. G The projected area A of the brick-red region in the PN / A granular sludge photograph R ;

[0066] S4. Calculate the overall inhibition P according to the following formula.

[0067]

[0068] In the formula, q represents the activity of granular sludge in wastewater, and q0 represents the activity of granular sludge in the control group.

[0069] This application uses an optical observation dish, a camera, and a data processing unit to determine the activity of PN / A granular sludge and to provide a more comprehensive evaluation of changes in overall kinetic activity and morphological characteristics. Compared with existing professional testing equipment, the equipment in this application is simple and efficient to operate.

[0070] Secondly, the main microorganisms in the PN / A granular sludge used in this application are AMX and AOB, which are characterized by simple composition and distinctive appearance. Moreover, compared with traditional activated sludge, which includes bacteria, fungi, protozoa, and metazoa, the microbial composition of this application is simpler, thus ensuring the stability and reliability of the results.

[0071] This application can quantitatively and accurately measure the overall biological inhibition of water samples and accurately evaluate changes in bacterial activity and particle morphology in PN / A granular sludge. Specific Implementation

[0073] Examples 1-4

[0074] Example 1

[0075] A method for determining the biological inhibitory properties of water samples includes the following steps:

[0076] S1. Inoculate 120 ml of PN / A granular sludge into the upflow reactor 1, turn on the peristaltic pump on the inlet pipe 5 to introduce water, the inlet ammonia nitrogen concentration is 100 mg / L, the pH is 7.8, the hydraulic retention time is 1 h, the water temperature is 25℃, turn on the aeration device 10, and control the DO concentration in the aeration tank to 0.6 mg / L.

[0077] The PN / A granular sludge has a particle size of 1.0 mm, an MLSS of 9.7 g / L, and a microbial composition including 37% aerobic ammonia oxidizing bacteria (AOB), 42% anaerobic ammonia oxidizing bacteria (AMX), and the remainder being symbiotic heterotrophic bacteria.

[0078] S2. The activity q of the sludge is measured every 5 days. Every 5 days, the sampling valve 7 is opened to discharge 4 ml of PN / A granular sludge from the sludge discharge pipe 6 into the optical observation dish 2. The sidewalls and bottom of the optical observation dish 2 are made of quartz glass plate.

[0079] S3. Use the camera 3 to take photos of PN / A granular sludge, and use Image Pro-Plus image processing software to identify and statistically analyze the vertical projected area of ​​the PN / A granular sludge displayed in the photos to obtain the overall projected area A of the PN / A granular sludge photos. G The projected area A of the brick-red region in the PN / A granular sludge photograph R ;

[0080] S4. Calculate the overall inhibition P according to the following formula.

[0081]

[0082] In the formula, q represents the activity of granular sludge in wastewater, and q0 represents the activity of granular sludge in the control group.

[0083] Example 2

[0084] S1. Inoculate 100 ml of PN / A granular sludge into the upflow reactor 1, turn on the peristaltic pump on the inlet pipe 5 to introduce water, the inlet ammonia nitrogen concentration is 100 mg / L, the pH is 7.8, the hydraulic retention time is 1 h, the water temperature is 25℃, turn on the aeration device 10, and control the DO concentration in the aeration tank to 0.8 mg / L.

[0085] The PN / A granular sludge has a particle size of 0.8 mm, an MLSS of 15 g / L, and a microbial composition including 35% aerobic ammonia oxidizing bacteria (AOB), 40% anaerobic ammonia oxidizing bacteria (AMX), and the remainder being symbiotic heterotrophic bacteria.

[0086] S2. The activity q of the sludge is measured every 5 days. Every 5 days, the sampling valve 7 is opened to discharge 6 ml of PN / A granular sludge from the sludge discharge pipe 6 into the optical observation dish 2. The side wall of the optical observation dish 2 is made of quartz glass plate and the bottom is made of plexiglass plate.

[0087] S3. Use the camera 3 to take photos of PN / A granular sludge, and use Image Pro-Plus image processing software to identify and statistically analyze the vertical projected area of ​​the PN / A granular sludge displayed in the photos to obtain the overall projected area A of the PN / A granular sludge photos. G The projected area A of the brick-red region in the PN / A granular sludge photograph R ;

[0088] S4. Calculate the overall inhibition P according to the following formula.

[0089]

[0090] In the formula, q represents the activity of granular sludge in wastewater, and q0 represents the activity of granular sludge in the control group.

[0091] Example 3

[0092] S1. Inoculate 100 ml of PN / A granular sludge into the upflow reactor 1, turn on the peristaltic pump on the inlet pipe 5 to introduce water, the inlet ammonia nitrogen concentration is 100 mg / L, the pH is 7.8, the hydraulic retention time is 1 h, the water temperature is 25℃, turn on the aeration device 10, and control the DO concentration in the aeration tank to 0.7 mg / L.

[0093] The PN / A granular sludge has a particle size of 1.1 mm, an MLSS of 10.5 g / L, and a microbial composition including 12% aerobic ammonia oxidizing bacteria (AOB), 46% anaerobic ammonia oxidizing bacteria (AMX), and the remainder being symbiotic heterotrophic bacteria.

[0094] S2. Measure the activity q of the sludge every 5 days. Open the sampling valve 7 every 5 days and discharge 4 ml of PN / A granular sludge from the sludge discharge pipe 6 into the optical observation dish 2. The side wall of the optical observation dish 2 is made of plexiglass and the bottom is made of quartz glass.

[0095] S3. Use the camera 3 to take photos of PN / A granular sludge, and use Image Pro-Plus image processing software to identify and statistically analyze the vertical projected area of ​​the PN / A granular sludge displayed in the photos to obtain the overall projected area A of the PN / A granular sludge photos. G The projected area A of the brick-red region in the PN / A granular sludge photograph R ;

[0096] S4. Calculate the overall inhibition P according to the following formula.

[0097]

[0098] In the formula, q represents the activity of granular sludge in wastewater, and q0 represents the activity of granular sludge in the control group.

[0099] Example 4

[0100] This embodiment provides a device and method for determining the biological inhibitory properties of water samples. The determination method includes the following steps:

[0101] S1. Inoculate 100 ml of PN / A granular sludge into the upflow reactor 1, turn on the peristaltic pump on the inlet pipe 5 to introduce water, the inlet ammonia nitrogen concentration is 100 mg / L, the pH is 7.8, the hydraulic retention time is 1 h, the water temperature is 25℃, turn on the aeration device 10, and control the DO concentration in the aeration tank to 0.6 mg / L.

[0102] The PN / A granular sludge has a particle size of 0.5 mm, an MLSS of 8 g / L, and a microbial composition including 32% aerobic ammonia oxidizing bacteria (AOB), 35% anaerobic ammonia oxidizing bacteria (AMX), and the remainder being symbiotic heterotrophic bacteria.

[0103] S2. The activity q of the sludge is measured every 5 days. Every 5 days, the sampling valve 7 is opened to discharge 5 ml of PN / A granular sludge from the sludge discharge pipe 6 into the optical observation dish 2. The sidewalls and bottom of the optical observation dish 2 are made of quartz glass plate.

[0104] S3. Use the camera 3 to take photos of PN / A granular sludge, and use Image Pro-Plus image processing software to identify and statistically analyze the vertical projected area of ​​the PN / A granular sludge displayed in the photos to obtain the overall projected area A of the PN / A granular sludge photos. GThe projected area A of the brick-red region in the PN / A granular sludge photograph R ;

[0105] S4. Calculate the overall inhibition P according to the following formula.

[0106]

[0107] In the formula, q represents the activity of granular sludge in wastewater, and q0 represents the activity of granular sludge in the control group.

[0108] The parameters for each step in Examples 1-4 are shown in Table 1 below.

[0109] Table 1

[0110] Example 1 Example 2 Example 3 Example 4 DO (mg / L) 0.6 0.8 0.7 0.6 Granular sludge particle size (mm) 1 0.8 1.1 0.5 MLSS (g / L) 9.7 15 10.5 8 Aerobic ammonia-oxidizing bacteria AOB (%) 37% 35% 12% 32% Anaerobic ammonia oxidizing bacteria (AMX) (%) 42% 40% 46% 35% Sludge sample (ml) 4ml 6ml 4ml 5ml

[0111] As can be seen from Examples 1-4, the device and method for measuring the biological inhibition of water samples used in this application have a simple structure and are highly operable.

[0112] Application Example 1

[0113] Application Example 1 provides a method for measuring municipal wastewater, using tap water as a control group and municipal wastewater as the test object.

[0114] Application Example 1 uses the method of Example 1 for the experiment, wherein the number of upflow reactors is 4, corresponding to tap water, effluent from municipal wastewater treatment plant A, effluent from municipal wastewater treatment plant B and effluent from municipal wastewater treatment plant C respectively.

[0115] Wastewater inhibition was evaluated using data from day 30, with a 30-day period as one cycle. The relevant data are shown in Table 2.

[0116] Table 2 Evaluation of the bioinhibition effect of different municipal wastewater on PN / A granular sludge

[0117] <![CDATA[q / q0]]> <![CDATA[A R / A G ]]> P Municipal sewage A 30.22 18.89 24.55 Municipal sewage B 44.12 28.57 36.34 Municipal sewage C 39.39 23.23 31.31

[0118] The effects of different municipal wastewater treatments on the nitrogen removal performance of PN / A granular sludge are shown in the figure. Figure 3 Different municipal wastewater treatments affect PN / A granular sludge A R / A G See the impact Figure 4 .

[0119] The experimental results in Table 2 show that, based on the P-value, the inhibitory strength of the three types of municipal wastewater is: municipal wastewater A > municipal wastewater C > municipal wastewater B.

[0120] Application Example 2

[0121] Application Example 2 provides a method for determining electroplating wastewater, using tap water as the control group and electroplating wastewater as the test object.

[0122] Application Example 2 uses the method of Example 3 for the experiment, wherein the number of upflow reactors is 3, corresponding to tap water, electroplating wastewater A effluent and electroplating wastewater B effluent respectively.

[0123] The wastewater inhibition effect was evaluated using data from day 30, with a 30-day period as one cycle. The relevant data are shown in Table 3.

[0124] Table 3 Evaluation of the bioinhibition effect of different electroplating wastewater on PN / A granular sludge

[0125] <![CDATA[q / q0]]> <![CDATA[A R / A G ]]> P Electroplating wastewater A 0.07 0.07 0.07 Electroplating wastewater B 0.08 0.1 0.09

[0126] The effects of different electroplating wastewater on the denitrification performance of PN / A granular sludge are shown in the figure. Figure 5 Different electroplating wastewater affects PN / A granular sludge A R / A G See the impact Figure 6 .

[0127] The experimental results in Table 3 show that, based on the P-value, the inhibitory strength of the two types of electroplating wastewater is: electroplating wastewater A > electroplating wastewater B.

[0128] Comparative Example 1

[0129] Comparative Example 1 uses the method disclosed in "Analysis of Sludge Morphology and Microbial Evolution Characteristics during Rapid Activation of Frozen PN / A Granular Sludge", Huang Ziheng et al., DOI: 10.13227 / j.hjkx.202104216.

[0130] The vertical projected area of ​​the PN / A granular sludge shown in the photographs of Example 1 and Comparative Example 1 was identified and statistically analyzed using Image Pro-Plus image processing software. The detection results are shown in Table 4 below.

[0131] Table 4

[0132] <![CDATA[A R / A G ]]> Example 1 0.86 Comparative Example 1 0.75

[0133] As shown in Table 4, Comparative Example 1 and Example 1 were compared by identifying the red projected area A of the PN / A granular sludge brick. R With the overall projected area A of the particles G The ratio used to determine the activity of granular sludge yields highly accurate test results. However, the method for determining the biological inhibition of water samples used in this application, while possessing high accuracy, also features a simple structure and quick and easy operation.

[0134] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A device for measuring the biological inhibition of water samples, characterized in that, It includes an upflow reactor (1), an optical observation dish (2), a camera (3), a data processing unit, and an LED light source (4); The upflow reactor (1) has an inlet pipe (5) at the bottom of one side and a sludge discharge pipe (6) at the bottom of the other side. The sludge discharge pipe (6) is equipped with a sampling valve (7). The inlet pipe (5) is connected to the sampling system (8). The inlet pipe (5) is equipped with a water pump (9). The bottom of the upflow reactor (1) is equipped with an aeration device (10). The top of the optical observation dish (2) is provided with a sample inlet (11), which is connected to the outlet end of the mud discharge pipe (6). The bottom of the optical observation dish (2) is made of light-transmitting material, and the side wall of the lower part of the optical observation dish (2) is recessed inward to form an installation groove (12). The LED light source (4) is installed in the mounting slot (12); The camera (3) is positioned below the optical observation dish (2), with the camera lens of the camera (3) facing the bottom of the optical observation dish (2), and the camera (3) is connected to the data processing unit; The upflow reactor (1) is equipped with a DO monitoring probe (13), a nitrate monitoring probe (14), a nitrite monitoring probe (15) and an ammonia nitrogen monitoring probe (16), which are respectively connected to the online water quality monitoring system (17).

2. The apparatus for determining the biological inhibition of water samples according to claim 1, characterized in that, The bottom of the optical observation dish (2) is square, the lower surface of the top of the optical observation dish (2) is a backlight surface, and the side walls and / or bottom of the optical observation dish (2) are made of quartz glass plate.

3. The apparatus for determining the biological inhibition of water samples according to claim 1, characterized in that, The length of the LED light source (4) is 3 / 4 to 4 / 5 of the bottom length of the optical observation dish (2).

4. The apparatus for determining the biological inhibition of water samples according to claim 1, characterized in that, The inner wall of the mounting groove (12) is provided with an internal thread, and the outer wall of the LED light source (4) is provided with an external thread that matches the internal thread.

5. A method for detecting the biological inhibition of a water sample using the apparatus for measuring the biological inhibition of a water sample as described in claim 1, characterized in that, Includes the following steps: S1. Inoculate PN / A granular sludge into the upflow reactor (1). The PN / A granular sludge is brick red. Introduce water and turn on the aeration device (10) to control the DO concentration in the aeration tank. S2. Based on the MLSS of the inoculated sludge, the activity q of the sludge is measured every 5 days. Every 5 days, the sampling valve (7) is opened to discharge 4~6 ml of PN / A granular sludge from the sludge discharge pipe (6) into the optical observation dish (2) so that it is evenly spread at the bottom of the optical observation dish (2). S3. Using the camera (3), take a picture of PN / A granular sludge. The data processing unit identifies and statistically analyzes the vertical projection area of ​​the PN / A granular sludge displayed in the PN / A granular sludge picture to obtain the overall projection area A of the PN / A granular sludge picture. G The projected area A of the brick-red region in the PN / A granular sludge photograph R ; S4. Calculate the overall inhibition P according to the following formula. In the formula, q represents the activity of granular sludge in wastewater, and q0 represents the activity of granular sludge in the control group. The microbial composition of the PN / A granular sludge includes 30-50% anaerobic ammonia oxidizing bacteria (AMX), 30-40% aerobic ammonia oxidizing bacteria (AOB), and the remainder being symbiotic heterotrophic bacteria. In step S1, the DO concentration in the aeration tank is controlled to be 0.6~0.8 mg / L.

6. The method for detecting biological inhibition in water samples using the apparatus for measuring biological inhibition of water samples according to claim 5, characterized in that, The PN / A granular sludge has a particle size of 0.5~2mm and an MLSS of 8~15g / L.

7. The method for detecting biological inhibition in water samples using the apparatus for measuring biological inhibition of water samples according to claim 5, characterized in that, In step S4, the calculation of the overall inhibition P uses data from day 30 to evaluate biological inhibition.

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