A kind of corn high temperature resistant composite regulator and its use method

Abstract

The invention discloses a corn growth composite regulator, which is an aqueous solution, and each 1kg of the composite regulator contains: 18-22mg of gibberellin, 10-20g of potassium dihydrogen phosphate, 10-20g of urea, 5-15g of amino acid foliar fertilizer, 0.1-0.5mg of brassinolide, and the balance is water. The product of the invention improves the corn pollination rate and yield by shortening the male-female interval of corn tasseling and silking. At the same time, the regulator of the invention also helps to increase the corn filling rate and accelerate the filling of grains, and is suitable for industrial production.

Description

Technical Field

[0001] The invention belongs to the technical field of crop planting, and particularly relates to a high temperature resistant composite regulator for corn and a use method thereof. Background Art

[0002] Corn is the second largest grain crop in Henan Province after wheat, with an annual planting area of ​​about 60 million mu. In 2022, the province's grain output reached 135.787 billion jin, and has remained stable at more than 130 billion jin for six consecutive years. The contribution rate of corn production reached 33.54%. The growth period of summer corn in our province is relatively short, and the frequent occurrence of high temperature and drought disastrous weather in production is the main limiting factor restricting the high-yield, high-quality and high-quality development of corn.

[0003] The annual corn planting area in our province is about 60 million mu, which is the second largest grain crop in our province after wheat. However, the wheat planting area is basically saturated, and the wheat output is at the leading level in the world. The wheat yield has basically reached the ceiling, and there is very limited room for improvement in the future. However, there is a large room for improvement in corn yield, and it is possible to further increase it on the basis of the current national average yield of 430 kg / mu. Research and analysis show that the frequent occurrence of natural disasters such as high temperature and drought during the corn growing season is one of the main factors limiting the further increase of corn yield. Therefore, enhancing the disaster resistance and mitigation capabilities of corn is the only way to achieve high and stable corn yields.

[0004] Spraying plant growth regulators is currently one of the main means to obtain high yields by improving crop resistance to stress. Previous researchers have conducted a lot of research on the application of chemical growth regulators in corn production, mainly focusing on improving corn lodging resistance and delaying aging. However, there are no reports on how to achieve high and stable corn yields through chemical regulation under high temperature and drought conditions. Summary of the invention

[0005] 1. Technical issues to be resolved

[0006] In view of the above-mentioned shortcomings and deficiencies of the prior art, the present invention provides a composite high temperature resistant regulator for corn, which can shorten the male-female interval of corn tasseling and silking, improve the corn pollination rate, increase the corn filling rate, accelerate the grain filling process, and increase the grain yield, thereby reducing the impact of high temperature heat damage during the tasseling and silking period on corn yield. The composite regulator of the present invention has simple components and is easy to produce. In addition, the present invention also relates to a method for using the composite high temperature resistant regulator for corn.

[0007] (II) Technical solution

[0008] In the first aspect, the present invention provides a corn high temperature resistant composite regulator, which is an aqueous solution, and each 1 kg of the corn high temperature resistant composite regulator contains: 18-22 mg of gibberellin, 20-30 g of potassium dihydrogen phosphate, 20-30 g of urea, 5-15 g of amino acid foliar fertilizer, and 0.1-0.5 mg of brassinolide.

[0009] According to a preferred embodiment of the present invention, at least 75 wt% of the amino acids in the amino acid foliar fertilizer are in the form of protein.

[0010] According to a preferred embodiment of the present invention, the mass content of each amino acid in the amino acid foliar fertilizer is as follows: aspartic acid 4% ± 0.4%, isoleucine 1.40% ± 0.1%, threonine 0.15% ± 0.05%, leucine 2.80% ± 0.2%, serine 0.27% ± 0.05%, tyrosine 0.44% ± 0.05%, glutamic acid 8.30% ± 0.8%, phenylalanine 1.90% ± 0.2%, glycine 23.5±2%, lysine 2.50±0.2%, alanine 9.90%±1%, histidine 0.53%±0.05%, cystine 1.63±0.1%, arginine 3.60%±0.3%, valine 2.30%±0.2%, proline 8.10%±0.8%, methionine 0.78%±0.05%, total amino acids 65.55-78.65%.

[0011] According to a preferred embodiment of the present invention, a synergistic composition is further added to the corn high temperature resistant composite regulator, and the synergistic composition includes a nano-colloidal solution, a sophorolipid and a dextrin; the nano-colloidal solution is a nano-colloidal solution of a methyl methacrylate-methacrylic acid copolymer, the colloid particle size is 10-100nm, and the final concentration of addition is 200-300mL / Kg; the solid content of the nano-colloidal solution is 50-68%; the final concentration of the sophorolipid is 16-24g / Kg; the final concentration of the dextrin is 20-30g / Kg.

[0012] According to a preferred embodiment of the present invention, the preparation method of the nano-colloidal solution is: first dissolving methyl methacrylate and methacrylic acid copolymer in tetrahydrofuran, stirring at room temperature to dissolve; adding 10 times of pure water, stirring continuously to obtain a clear and transparent colloidal solution; removing tetrahydrofuran and part of the water by rotary evaporation to obtain a nano-colloidal solution with a solid content of 50-68%.

[0013] According to a preferred embodiment of the present invention, the corn high temperature resistant composite regulator also contains 0.5-0.8wt% of microalgae extracellular metabolites, and the microalgae extracellular metabolites are heterotrophically cultured Chlorella extracellular metabolites. The main components of heterotrophic Chlorella extracellular metabolites are organic acids or esters, phenols, glycosides and sugars. After heterotrophically culturing Chlorella, the supernatant is collected, concentrated, frozen, and made into powdered metabolites, and the powdered metabolites are added to the corn high temperature resistant composite regulator.

[0014] When preparing the corn high temperature resistant composite regulator, each component is weighed and mixed evenly in pure water to obtain the corn high temperature resistant composite regulator.

[0015] In a second aspect, the present invention provides a method for using a high temperature resistant composite regulator for corn, wherein the high temperature resistant composite regulator for corn is sprayed 1-2 weeks before corn tasseling and silking;

[0016] Spraying method: spray 1kg of the corn high temperature resistant composite regulator per mu of land, and spray after evenly mixing the corn high temperature resistant composite regulator and water in a mass ratio of 1:13-17, mainly spraying corn cobs with three leaves or more.

[0017] In a third aspect, the present invention provides a method for alleviating the impact of high temperature heat damage on corn yield, comprising: spraying the corn high temperature resistance composite regulator on corn.

[0018] According to a preferred embodiment of the present invention, the spraying is carried out 1-2 weeks before corn tasseling and silking, and 1 kg of the corn high temperature resistant composite regulator is sprayed per acre. When in use, the corn high temperature resistant composite regulator and water are mixed in a mass ratio of 1:13-17 and then sprayed, and the corn cobs with three leaves or more are mainly sprayed.

[0019] (III) Beneficial effects

[0020] The high temperature resistant composite regulator of corn of the present invention contains a certain concentration of gibberellins, potassium dihydrogen phosphate, urea, amino acid foliar fertilizer, and brassinolide; its main purpose is to reduce the impact of high temperature heat damage during the tasseling and silking period of corn on corn yield. Summer corn often encounters high temperature and heat damage weather during the flowering period, which causes the corn to fail to tassel or silk in time, or the silking period is delayed after tasseling, resulting in a missed flowering period. The product of the present invention improves the pollination rate of corn by shortening the male-female interval period of corn tasseling and silking. At the same time, the regulator of the present invention also helps to increase the corn filling rate, accelerate the grain filling process, and increase the grain yield, and is suitable for industrial production.

[0021] In some preferred embodiments of the present invention, the corn high temperature resistant composite regulator is added with a synergistic composition, which includes a nano-colloidal solution, a sophorolipid and a dextrin. On the one hand, the corn high temperature resistant composite regulator can be stabilized and the uniformity of its components can be maintained; on the other hand, the residence time of the effective ingredients of the corn high temperature resistant composite regulator on the corn leaves can be prolonged. Gibberellic acid can promote stem elongation, stimulate cell elongation, delay cell senescence, promote male flower differentiation, prevent falling flowers and fruits, and increase fruit yield. Brassinolide promotes crop growth and increases crop yield, improves the cold tolerance of crops, improves the disease resistance and salt resistance of crops, enhances the stress tolerance of crops, and reduces the phytotoxicity of herbicides to crops. By adding the synergistic composition, gibberellins and brassinolide are slowly released, regulating plant growth, increasing the growth of plant nutrients and promoting fertilization, and reducing its side effects. The synergistic composition also has a very stable moisture content, which prevents the leaves from losing water and withering rapidly under high temperature heat damage.

[0022] In some other preferred embodiments of the present invention, the corn high temperature resistant composite regulator is also added with a certain amount of microalgae extracellular metabolites (including microalgae extracellular polysaccharides). The microalgae extracellular metabolites are a natural biostimulant that is non-toxic, harmless, and degradable, which is beneficial to enhancing the stress resistance of corn crops and alleviating the impact of high temperature heat damage during the tasseling and silking period of corn on corn yield. DETAILED DESCRIPTION

[0023] In order to better explain the present invention and facilitate understanding, the present invention is described in detail below in conjunction with specific implementation methods.

[0024] In the following examples, the mass content of each amino acid in the amino acid foliar fertilizer is as shown in Table 1:

[0025] Table 1: Amino acid content in amino acid foliar fertilizer

[0026]

[0027]

[0028] In order to further illustrate the technical features and effects of the present invention, a comparative explanation is given below in combination with specific embodiments and comparative examples.

[0029] Examples 1-4

[0030] The following are the components contained in 1 kg of corn high temperature resistant composite regulator (Table 2):

[0031] Group Gibberellins Potassium dihydrogen phosphate Urea Brassinolide Amino acid foliar fertilizer Example 1 20mg 15g 15g 0.3mg 7g Example 2 18mg 20g 10g 0.1mg 15g Example 3 22mg 10g 20g 0.5mg 5g Example 4 20mg 15g 15g 0.3mg 10g

[0032] Comparative Example 1-2

[0033] The following comparative examples are the components contained in 1 kg of corn high temperature resistant composite regulator (Table 3):

[0034] Group Gibberellins Potassium dihydrogen phosphate Urea Brassinolide Amino acid foliar fertilizer Comparison 1 20mg 15g 15g / / Comparison 2 20mg 15g 15g 0.3mg /

[0035] Comparative Examples 3-4

[0036] The following comparative examples are the components contained in 1 kg of corn high temperature resistant composite regulator (Table 4):

[0037] Group Gibberellins Potassium dihydrogen phosphate Urea 6-Benzylaminoadenine Amino acid foliar fertilizer Contrast 3 20mg 15g 15g 0.3mg / Contrast 4 20mg 15g 15g 0.3mg 10g

[0038] The composite regulators of Examples 1-4 and Comparative Examples 1-4 were used to spray corn, and normal light and high temperature treatments were used as references for spraying clean water, with a total of 10 treatments, each repeated 3 times. Among them, various composite regulators were sprayed 3-4 days before silking (according to the tests in the previous two years, the spraying effect before silking was the best, 1 kg of corn high temperature resistant composite regulator was sprayed per mu of land, and the corn high temperature resistant composite regulator and water were mixed evenly at a mass ratio of 1:15, and mainly sprayed on corn cobs with three leaves or more), and the test variety was Liyu 16.

[0039] 1. Effect of high temperature treatment on microclimate of summer corn population

[0040] The high temperature treatment shed (building a high temperature heat damage environment) is 8.5 meters high, 40 meters long, and 10 meters wide, and is set in the north-south direction. A high temperature treatment net is built on the top of the high temperature treatment shed to ensure that the field microclimate in the high temperature treatment shed is basically consistent with normal light. The light radiation, temperature and CO2 concentration under the high temperature treatment shed and normal light conditions are shown in Table 5.

[0041] Table 5: Comparison of microclimate of corn colonies under high temperature treatment and normal light

[0042]

[0043] 2. Output

[0044] Table 6: Differences in yields treated with different composite regulators in Examples 1-4 and Comparative Examples 1-4.

[0045] Group Yield Group Yield Example 1 647.3 kg / mu Example 2 653.4 kg / mu Example 3 642.7 kg / mu Example 4 648.9 kg / mu Comparative Example 1 523.5 kg / mu Comparative Example 2 581.6 kg / mu Comparative Example 3 556.8 kg / mu Comparative Example 4 609.2 kg / mu Normal lighting 747.8 kg / mu Spraying water control 448.2 kg / mu

[0046] It can be seen from the above table that under normal lighting conditions (non-high temperature heat damage environment), the corn yield per mu reached 747.8 kg, the yield of the high temperature treatment sprayed with clean water control treatment was 448.2 kg / mu, and the high temperature (heat damage) treatment before silking (high temperature spraying of clean water treatment started before silking, and high temperature treatment lasted for 7 days) decreased by 40.07% compared with the normal light treatment.

[0047] The yield of the treatments of spraying different combinations of compound control agents increased to varying degrees, with the largest increase in Example 2, and Examples 1, 3-4 were also relatively high. The average yield of Examples 1-4 reached 648.08 kg / mu, which was 44.6% higher than the yield of spraying water treatment, but the yield was still reduced by 13.33% compared with the normal light treatment. The treatments of different combinations of agents increased the yield, but the degree of increase was different, and the order was: Example 2> Example 4> Example 1> Example 3> Comparative Example 4> Comparative Example 2> Comparative Example 3> Comparative Example 1.

[0048] 3. Yield components and ear characteristics

[0049] Table 7 shows the effects of different composite regulators on the yield components and ear traits of corn under high temperature treatment. The results show that the main reason for the decrease in yield in high temperature treatment (spraying water control) is the shortening of ear length, the lengthening of bald tip, the reduction of row grain number, the reduction of ear grain number and the decrease of 1000-grain weight, which is consistent with the previous research results. The yield of each embodiment of the present invention is the highest, mainly because the ear length is increased, the bald tip is reduced, the row grain number and the ear grain number are increased, and the 1000-grain weight is also significantly increased, wherein the ear grain number and the 1000-grain weight of embodiment 4 are increased by 28.5% and 12.57% respectively compared with the spraying water treatment; wherein the ear grain number and the 1000-grain weight of embodiment 2 are increased by 28.6% and 12.8% respectively compared with the spraying water treatment.

[0050] Table 7: Effects of different compound regulators on maize yield components and ear traits under high temperature treatment

[0051]

[0052] 4. Dry matter accumulation

[0053] Table 8 shows the effect of spraying composite regulator on the dry matter weight of individual plants at different growth stages. The results are as follows: the dry matter of individual plants in high temperature treatment (spraying water control) was significantly reduced, and the dry matter in the milky stage decreased by 30.1% compared with the normal light treatment. Spraying composite regulator increased the dry matter weight of individual plants, mainly in the middle filling and milky stages, and the largest yield increase was 5.43% and 33.0% higher than the spraying water control group (in the middle filling and milky stages); and the dry matter weight of individual plants in embodiment 4 was increased by 5.58% and 33.3% respectively compared with the spraying water control group (in the middle filling and milky stages).

[0054] Table 8 Effect of spraying compound regulator on dry matter weight of single plant at different growth stages (g)

[0055]

[0056] The changes in the accumulation of dry matter weight in different organs of individual plants were analyzed (data in Table 9), and it was found that under the high temperature treatment (water spraying group), the dry matter accumulation of leaves and stem sheaths was higher after the middle of grain filling than that under the normal light treatment. It can be seen that under the high temperature treatment, the carbohydrates stored in the leaves and stem sheaths could not be fully converted into carbohydrates in the grains after flowering and accumulated, that is, the re-transport rate of carbohydrates in the leaves and stem sheaths during the grain filling period decreased.

[0057] The main effect of spraying compound regulators is to improve the re-transport capacity of carbohydrates in leaves and stem sheaths, reduce the dry matter weight of leaves and stem sheaths during the filling period to varying degrees, and increase the dry matter accumulation of grains in the ear.

[0058] Table 9 Effect of spraying compound regulator on dry matter weight (g) of single plant at different growth stages

[0059]

[0060] 5. Mechanical properties of stems

[0061] Table 10 shows the effect of spraying the composite regulator on the mechanical properties of the third internode at the base at different growth stages. High temperature treatment 3-4 days before silking (spraying water group) has almost no effect on the mechanical properties of the third internode at the base of corn (normal light). The effect of spraying the composite regulator on the mechanical characteristics of the third internode at the base is also small, and it has a certain effect on improving the lodging resistance of the third internode at the base. In the milky stage, the puncture resistance, crushing strength and bending strength of the plant in Example 2 were increased by 6.89%, 1.35% and 5.26% respectively compared with the spraying water group.

[0062] (1) Mechanical characteristics of the third internode at the base

[0063] Table 10: Effects of spraying compound regulators on mechanical properties of the third internode at different growth stages (N / mm 2 )

[0064]

[0065] (2) Mechanical characteristics of the internode stem below the ear

[0066] High temperature treatment has a great influence on the mechanical properties of the internodes below the ear. As can be seen from Table 11, compared with the normal light, the puncture strength, crushing strength and flexural strength of the high temperature spraying water group decreased to varying degrees in the middle filling stage and milky stage. Among them: the middle filling stage decreased by 34.66%, 18.67% and 11.37% respectively; the milky stage decreased by 23.61%, 18.21% and 14.15% respectively.

[0067] Spraying various compound regulators can effectively improve the lodging resistance of the internodes under the ear, and the puncture strength, crushing strength and bending strength are all improved to varying degrees. Among them, the treatment with the highest yield (Example 2) increased by 40%, 18.9%, and 22.89% respectively in the middle filling period compared with the clear water control treatment (spraying clear water group); and increased by 21.74%, 12.2%, and 10.35% respectively in the milky stage.

[0068] Table 11 Effects of spraying compound regulators on mechanical properties of internodes below the ear at different growth stages (N / mm 2 )

[0069]

[0070] In summary, by spraying a high-temperature resistant composite regulator composed of gibberellin 18-22 mg / kg + potassium dihydrogen phosphate 1.5-2.0% + urea 1.5-2.0% + brassinolide 0.1-0.5 mg / L + amino acid foliar fertilizer 5-15 g / kg, the corn yield under simulated high temperature (high-temperature treatment shed) treatment can be significantly increased, which is 44.6% higher than the control yield sprayed with clear water. The reason is that the number of grains per ear is increased and the occurrence of bald tips is reduced.

[0071] Under high temperature treatment, the lodging resistance of the internodes under the ear decreases, and the puncture strength, crushing strength and bending strength all decrease to varying degrees, while the lodging resistance of the base internodes does not change much. By spraying the high temperature resistant composite regulating agent of the present invention, the lodging resistance of the internodes under the ear can be effectively improved. In Example 2, three stem mechanical property indicators (puncture strength, crushing strength and bending strength) were sprayed with clean water during the milky stage, and they were increased by 21.74%, 12.2% and 10.35%, respectively.

[0072] Comparative Examples 5-6

[0073] Comparative Example 5 is to replace the 20mg / kg gibberellin in Comparative Example 1 with 0.2mg / L indoleacetic acid. Comparative Example 6 is: 20mg / kg gibberellin + 10g / kg amino acid foliar fertilizer. The experiment shows that the effect of Comparative Examples 5-6 on reducing high temperature heat damage on corn yield is not as good as Comparative Example 1.

[0074] The ear setting rates of Example 1, Example 6 and Example 5 treated with high temperature and sprayed with growth regulators increased by 17.61%, 11.95% and 9.43% respectively compared with the high temperature treatment; the corresponding number of grains per ear increased by 11.91%, 10.88% and 13.23% respectively.

[0075] Example 5

[0076] The high temperature resistant composite regulator of corn in this embodiment is: 20mg gibberellin, 15g potassium dihydrogen phosphate, 15g urea, 0.3mg brassinolide, 7g amino acid foliar fertilizer, 260mL of nano-colloidal solution of methyl methacrylate-methacrylic acid copolymer (colloid particle size is 10-100nm, solid content is 55wt%), 20g sophorolipid, and 25g dextrin per 1kg composite regulator. The preparation method of the nano-colloidal solution is: first dissolve methyl methacrylate and methacrylic acid copolymer in tetrahydrofuran, stir and dissolve at room temperature; add 10 times pure water, and stir continuously to obtain a clear and transparent colloidal solution; remove tetrahydrofuran and part of the water by rotary evaporation to obtain a nano-colloidal solution with a solid content of 55%.

[0077] Example 6

[0078] In Example 5, the extracellular metabolites of Chlorella were added in an amount of 0.6 wt %. The extracellular metabolites were extracellular metabolites of heterotrophically cultured Chlorella. The main components of the heterotrophic extracellular metabolites of Chlorella were organic acids or esters, phenols, glycosides and sugars. After heterotrophically culturing Chlorella, the supernatant was collected, concentrated, and frozen to make powdered metabolites.

[0079] The composite regulators of Examples 5 and 6 were sprayed on corn. Compared with Example 2, each group was repeated 3 times, and the experimental area of ​​each plot was 17.5 m 2 Among them, various compound regulators were sprayed 3-4 days before silking (1 kg of corn high temperature resistant compound regulator was sprayed per mu of land, the mass ratio of regulator to water was 1:15, and the corn cobs with three leaves or more were mainly sprayed). The test variety was Liyu 16. The yield comparison is shown in Table 12:

[0080] Table 12: Difference in yield under treatment with composite regulators in Examples 5 and 2

[0081] Group Yield Group Yield Example 5 677.2 kg / mu Example 2 653.4 kg / mu Example 6 682.8 kg / mu

[0082] It can be seen from the above table that, on the basis of the corn high temperature resistance composite regulator in Example 2, after adding a certain amount of the synergistic composition composed of nanocolloid solution, sophorolipid and dextrin, the corn yield is further improved. After adding the extracellular metabolites of microalgae, the high temperature resistance of corn crops is also improved to a certain extent, and the per mu yield is increased.

[0083] Real production application examples

[0084] In 2021-2022, two thousand-acre plots of Zhengdan 6122 and Zhengdan 7137 varieties were established in Xihua County, Dancheng County, Taikang County, and Wuyang County. The high-temperature resistant composite regulator of corn in Example 2 was sprayed 1-2 weeks before corn tasseling and silking, and 1 kg of corn high-temperature resistant composite regulator was sprayed per mu of land. When used, the mass ratio of the high-temperature resistant composite regulator to water was 1:15, and then mixed and sprayed. The corn cob was mainly sprayed with three leaves and above. The demonstration of the thousand-acre plot was carried out. After the expert production measurement, the yield of Zhengdan 6122 reached 804.56 kg / mu (the control yield of spraying water was 602.34 kg / mu), and the yield of Zhengdan 7137 reached 753.45 kg / mu. The Zhengdan 6122 and Zhengdan 7137 corn varieties were both sprayed with the high-temperature resistant composite regulating agent of the present invention during the period from big mouth to silking by means of foliar fertilizer control spraying with drones. Especially in August 2022, when encountering rare high temperature weather, spraying the high-temperature resistant composite regulating agent helped to improve male and female coordination, achieve good pollination, and avoid the phenomenon of bald flower tips and grains.

[0085] Although the solutions of Examples 5-6 have not yet been put into field production application, the existing small-scale tests have shown that they have better effects on enhancing the resistance of corn to high temperature heat damage than Examples 1-4, and reduce the problem of corn yield reduction caused by high temperature heat damage.

[0086] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A composite regulator for high temperature resistance of corn, characterized in that: The corn high temperature resistant composite regulator is an aqueous solution, and each 1 kg of the corn high temperature resistant composite regulator contains: 18-22 mg of gibberellin, 20-30 g of potassium dihydrogen phosphate, 20-30 g of urea, 5-15 g of amino acid foliar fertilizer, and 0.1-0.5 mg of brassinolide; the corn high temperature resistant composite regulator is further added with a synergistic composition, and the synergistic composition includes a nano-colloidal solution, sophorolipids and dextrin; the nano-colloidal solution is a nano-colloidal solution of methyl methacrylate-methacrylic acid copolymer, the colloidal particle size is 10-100 nm, and the final added concentration is 200-300 mL / Kg; the solid content of the nano-colloidal solution is 50-68%; the final concentration of sophorolipids is 16-24 g / Kg; and the final concentration of dextrin is 20-30 g / Kg.

2. The high temperature resistant composite regulator for corn according to claim 1, characterized in that: The mass content of each amino acid in the amino acid foliar fertilizer is as follows: aspartic acid 4% ± 0.4%, isoleucine 1.40% ± 0.1%, threonine 0.15% ± 0.05%, leucine 2.80% ± 0.2%, serine 0.27% ± 0.05%, tyrosine 0.44% ± 0.05%, glutamic acid 8.30% ± 0.8%, phenylalanine 1.90% ± 0.2%, glycine 0. amino acid 23.5±2%, lysine 2.50±0.2%, alanine 9.90%±1%, histidine 0.53%±0.05%, cystine 1.63±0.1%, arginine 3.60%±0.3%, valine 2.30%±0.2%, proline 8.10%±0.8%, methionine 0.78%±0.05%, total amino acids 65.55-78.65%.

3. The high temperature resistant composite regulator for corn according to claim 1, characterized in that: The corn high temperature resistant composite regulator also contains 0.5-0.8 wt % of microalgae extracellular metabolites, and the microalgae extracellular metabolites are extracellular metabolites of heterotrophically cultured Chlorella.

4. A method for using a high temperature resistant composite regulator for corn, characterized in that: Spraying the corn high temperature resistant composite regulator according to any one of claims 1 to 3 1 to 2 weeks before corn tasseling and silking; Spraying method: spray 1kg of the corn high temperature resistant composite regulator per mu of land, and spray after evenly mixing the corn high temperature resistant composite regulator and water in a mass ratio of 1:13-17, mainly spraying corn cobs with three leaves or more.

5. A method for alleviating the impact of high temperature heat damage on corn yield, characterized in that: include: Spray the corn high temperature resistance composite regulator according to any one of claims 1 to 3 on the corn.

6. The method according to claim 5, characterized in that Spray the corn 1-2 weeks before it tassels and silks. Spray 1kg of corn high-temperature resistant compound regulator per acre. Mix the corn high-temperature resistant compound regulator and water in a mass ratio of 1:13-17 and spray it. Mainly spray the leaves of corn cobs with three leaves or more.