Single molecule conductance measurement system, control method thereof, and readable storage medium

Abstract

Embodiments of the present application provide a single molecule conductance measurement system, a control method thereof and a readable storage medium. The control device of the single molecule conductance measurement system is electrically connected with a conductance measurement device, a sample transportation device and a reagent dropping device. The sample transportation device can be controlled by the control device to transport the sample to be measured to the working area of the reagent dropping device. When the sample to be measured is detected in the working area of the reagent dropping device, the reagent dropping device is controlled to drop the target reagent into the sample to be measured. After the sample to be measured after the target reagent is dropped is transported to the working area of the conductance measurement device by the transportation device, the conductance measurement device is controlled to measure the conductance of the sample to be measured. The whole process does not need personnel control operation, or the operator can remotely measure the conductance of the sample to be measured by the control device, effectively solving the technical problems of low automation and low measurement accuracy of the single molecule conductance measurement instrument in the prior art.

Description

Technical Field

[0001] This invention relates to the field of automation technology, and in particular to a single-molecule conductivity measurement system and its control method, as well as a readable storage medium. Background Technology

[0002] Currently, the electrotransport properties of single molecules are mainly studied using scanning tunneling microscopy break junction (STM-BJ) technology.

[0003] However, current instruments utilizing this technology suffer from low automation. In single-molecule experiments, multiple steps, such as tip loading, substrate replacement, sample addition, and parameter adjustment, require manual operation, resulting in low efficiency. Furthermore, external factors such as mechanical vibration and electromagnetic interference can easily cause unstable measurement data during instrument operation.

[0004] Therefore, current instruments based on STMBJ technology generally suffer from low automation, require personnel to remain on-site for control, and are susceptible to external environmental influences on experimental results. Furthermore, the use of volatile or toxic chemical reagents during experiments inevitably poses a potential threat to the health of on-site observers. Summary of the Invention

[0005] This invention provides a single-molecule conductivity measurement system, its control method, and a readable storage medium, thereby solving the technical problems of low automation and low measurement accuracy in existing single-molecule conductivity measurement instruments.

[0006] In a first aspect, the present invention provides a single-molecule conductivity measurement system through an embodiment of the present invention. The single-molecule conductivity measurement system includes a control device and a conductivity measurement device, a sample transport device, and a reagent dispensing device electrically connected to the control device; wherein the control device is used for:

[0007] The sample transport device is controlled to transport the sample to be tested to the working area of ​​the reagent dispensing device, and when the sample to be tested is detected to be in the working area of ​​the reagent dispensing device, the reagent dispensing device is controlled to add the target reagent to the sample to be tested.

[0008] The transport device is controlled to transport the sample to be tested after the target reagent has been added to it to the working area of ​​the conductivity measuring device, and the conductivity measuring device is controlled to perform conductivity measurement on the sample to be tested.

[0009] Optionally, the sample transport device includes:

[0010] A substrate array includes a substrate array base plate and a plurality of substrates disposed on the substrate array base plate; wherein, each substrate has a conductive sheet and a sample support sheet disposed on its upper surface, the sample support sheet being electrically connected to the conductive sheet; the sample support sheet is used to hold the sample to be tested;

[0011] The first linear module is fixedly connected to the base plate of the base array, and the first linear module reciprocates in a fixed direction.

[0012] Optionally, the conductivity measuring device includes:

[0013] The second linear module is equipped with a fixed plate, and the motion trajectory of the second linear module is perpendicular to the motion trajectory of the first linear module.

[0014] The conductivity measuring device is fixedly connected to the fixed plate through a third linear module, and the motion trajectory of the third linear module is on the same vertical plane as the motion trajectory of the first linear module.

[0015] Optionally, the conductivity measuring device includes:

[0016] A piezoelectric sensor, wherein the base of the piezoelectric sensor is disposed on the third linear module, and the signal output terminal of the piezoelectric sensor is electrically connected to the control device;

[0017] The probe is magnetically connected to the protective cover of the piezoelectric sensor.

[0018] Optionally, the conductivity measuring device further includes:

[0019] A biasing mechanism is provided, comprising a base and a pressure rod. The base is fixedly connected to the second linear module. The pressure rod is composed of two swing arms connected together, and the connection between the two swing arms is rotatably connected to the base via a pin. One swing arm is connected to the base by a spring, and the other swing arm is provided with a conductive protrusion.

[0020] When the conductive protrusion comes into contact with the conductive sheet of the sample transport device, an electrical connection is established between the conductive protrusion, the conductive sheet, and the sample support sheet.

[0021] Optionally, a concave guide rail is provided between two adjacent substrates; when the conductive protrusion is pressed against the concave guide rail, the conductive protrusion restricts the movement of the sample transport device.

[0022] Optionally, the reagent dispensing device includes:

[0023] The drip gun is fixedly connected to the mounting bracket via a fourth linear module; the movement trajectory of the fourth linear module and the movement trajectory of the sample support sheet are on the same vertical plane.

[0024] Optionally, the fourth linear module includes an X-axis sub-linear module and a Z-axis sub-linear module; the drip gun is fixedly connected to the X-axis sub-linear module, and the X-axis sub-linear module is fixed to the Z-axis sub-linear module;

[0025] The motion trajectory of the X-direction sub-linear module is consistent with the motion trajectory of the second linear module, and the motion trajectory of the Z-direction sub-linear module is on the same vertical plane as the motion trajectory of the first linear module.

[0026] The reagent dispensing device also includes a dispensing gun array, which has multiple grids, each grid being equipped with a dispensing gun.

[0027] In a second aspect, the present invention provides a control method for a single-molecule conductivity measurement system through an embodiment of the present invention, applied to the single-molecule conductivity measurement system in the first aspect, the method comprising:

[0028] The sample transport device is controlled to transport the sample to be tested to the working area of ​​the reagent dispensing device, and when the sample to be tested is detected to be in the working area of ​​the reagent dispensing device, the reagent dispensing device is controlled to add the target reagent to the sample to be tested.

[0029] The transport device is controlled to transport the sample to be tested after the target reagent has been added to it to the working area of ​​the conductivity measuring device, and the conductivity measuring device is controlled to perform conductivity measurement on the sample to be tested.

[0030] Thirdly, through one embodiment of the present invention, a readable storage medium is provided having a program stored thereon, which, when executed by a processor, implements any of the embodiments in the second aspect.

[0031] One or more technical solutions provided in the embodiments of the present invention have at least the following technical effects or advantages:

[0032] In this embodiment of the invention, the control device of the single-molecule conductivity measurement system is electrically connected to the conductivity measurement device, the sample transport device, and the reagent dispensing device. The control device can control the sample transport device to transport the sample to be tested to the working area of ​​the reagent dispensing device, and when the sample to be tested is detected to be within the working area of ​​the reagent dispensing device, control the reagent dispensing device to add the target reagent to the sample. Furthermore, after controlling the transport device to transport the sample to be tested with the added target reagent to the working area of ​​the conductivity measurement device, control the conductivity measurement device to perform conductivity measurement on the sample. The entire process requires no manual operation, or the operator can remotely perform conductivity measurement on the sample through the control device, effectively solving the technical problems of low automation and low measurement accuracy in existing single-molecule conductivity measurement instruments. Attached Figure Description

[0033] To more clearly illustrate the technical solutions in the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0034] Figure 1 This is a schematic diagram of the single-molecule conductivity measurement system in an embodiment of the present invention;

[0035] Figure 2 This is a front view of the structure of a single-molecule conductivity measurement system according to one embodiment;

[0036] Figure 3 This is a top view of the structure of a single-molecule conductivity measurement system according to one embodiment;

[0037] Figure 4 This is a side view of the structure of a single-molecule conductivity measurement system according to one embodiment;

[0038] Figure 5 This is a schematic diagram of the substrate array structure in an embodiment of the present invention;

[0039] Figure 6 This is a schematic diagram of the biasing mechanism in the conductivity measuring device according to an embodiment of the present invention;

[0040] Figure 7 This is a flowchart of the control method for the single-molecule conductivity measurement system in an embodiment of the present invention;

[0041] Figure 8 This is a schematic diagram of a readable storage medium structure in an embodiment of the present invention. Detailed Implementation

[0042] This invention provides a single-molecule conductivity measurement system, its control method, and a readable storage medium, thereby solving the technical problems of low automation and low measurement accuracy in existing single-molecule conductivity measurement instruments.

[0043] The technical solution provided by the embodiments of the present invention is to solve the above-mentioned technical problems, and the general idea is as follows:

[0044] By setting up a control device and establishing electrical connections between the control device and the conductivity measuring device, sample transport device, and reagent dispensing device, the control device can control the sample transport device to transport the sample to be tested to the working area of ​​the reagent dispensing device. Furthermore, when the sample to be tested is detected to be within the working area of ​​the reagent dispensing device, the control device can add the target reagent to the sample. Then, after the transport device has added the target reagent and transported the sample to the working area of ​​the conductivity measuring device, the control device can be controlled to measure the conductivity of the sample.

[0045] To better understand the above technical solutions, the following will provide a detailed explanation of the technical solutions in conjunction with the accompanying drawings and specific implementation methods.

[0046] First, it should be clarified that the term "and / or" in this article is merely a description of the relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent: A existing alone, A and B existing simultaneously, or B existing alone. Additionally, the character " / " in this article generally indicates that the preceding and following related objects have an "or" relationship.

[0047] It should be noted that the terms "first," "second," etc., in the specification, claims, and accompanying drawings of this invention are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be interchanged where appropriate so that embodiments of the invention described herein can be implemented in sequences other than those illustrated or described herein.

[0048] In a first aspect, the present invention provides a single-molecule conductivity measurement system through an embodiment of the invention, which can be applied to the study of single-molecule electrical transport properties. Please refer to [example missing]. Figure 1 As shown, the single-molecule conductivity measurement system includes: a control device 100 and a conductivity measurement device 200, a sample transport device 300, and a reagent dispensing device 400 electrically connected to the control device 100.

[0049] The control device 100 is configured to: control the sample transport device 300 to transport the sample to be tested to the operating area of ​​the reagent dispensing device 400, and, when detecting that the sample to be tested is within the operating area of ​​the reagent dispensing device 400, control the reagent dispensing device 400 to add the target reagent to the sample to be tested; and control the transport device to transport the sample to be tested after the target reagent has been added to the operating area of ​​the conductivity measuring device 200, and control the conductivity measuring device 200 to perform conductivity measurement on the sample to be tested.

[0050] Specifically, the control device 100 can be an electronic device with control functions. In one embodiment, the control device 100 can be a PLC (Programmable Logic Controller). Of course, the control device 100 can also be a host computer.

[0051] For details regarding the sample transport device 300, please refer to [reference needed]. Figure 2-4 As shown, the sample transport device 300 may include a substrate array 301 and a first linear module 302. See also... Figure 5 As shown, the substrate array 301 includes a substrate array base plate 3011 and a plurality of substrates 3012 disposed on the substrate array base plate 3011. Each substrate 3012 has a conductive sheet 3013 and a sample support sheet 3014 disposed on its upper surface. The sample support sheet 3014 is electrically connected to the conductive sheet 3013 and is used to hold the sample to be tested. A first linear module 302 is fixedly connected to the substrate array base plate 3011, and the first linear module 302 reciprocates in a fixed direction.

[0052] In the specific implementation process, the conductive sheet 3013 can be a copper conductive sheet, and the sample support sheet 3014 can be a gold sheet.

[0053] For details regarding the conductivity measuring device 200, please refer to [reference needed]. Figure 2-4 As shown, the conductivity measuring device 200 may include a second linear module 201 and a conductivity measuring device 202. The second linear module 201 is provided with a fixing plate 2011, and the movement trajectory of the second linear module 201 is perpendicular to the movement trajectory of the first linear module 302. The conductivity measuring device 202 is fixedly connected to the fixing plate 2011 through a third linear module, and the movement trajectory of the third linear module is on the same vertical plane as the movement trajectory of the first linear module 302.

[0054] To reduce vibrations caused by external factors and improve the stability of the conductivity measuring device 200 during movement, thereby improving the accuracy of the conductivity measuring instrument 202, in a specific implementation, the second linear module 201 can be mounted on the vibration damping table 500. The second linear module 201 can include two sub-linear modules connected by a fixing plate 2011. By synchronizing the movement of these two sub-linear modules, stable movement of the conductivity measuring instrument 202 is achieved.

[0055] In the specific implementation process, please continue to refer to, for example... Figure 2-4 As shown, the conductivity measuring device 202 may include a piezoelectric sensor 2021, a probe 2022, and a third linear module 2023. The base of the piezoelectric sensor 2021 is mounted on the third linear module 2023, and the signal output terminal of the piezoelectric sensor 2021 is electrically connected to the control device 100. The probe 2022 is magnetically connected to the protective cover of the piezoelectric sensor 2021.

[0056] In one alternative implementation, the piezoelectric sensor 2021 may be a piezoelectric ceramic.

[0057] To reduce sample jitter during conductivity measurements, it is necessary to both fix the substrate 3012 array 301 and allow for movement of the substrate 3012 array 301 under the drive of the first linear module 302. For details, please refer to... Figure 6 As shown, the conductivity measuring device 200 provided in this embodiment of the invention may further include a biasing mechanism 203. The biasing mechanism 203 consists of a base 2031 and a pressure rod 2032.

[0058] The base 2031 is fixedly connected to the second linear module 201. Specifically, the base 2031 is fixed to the vibration damping platform 500 on which the second linear module 201 is fixed. Please continue to refer to... Figure 6 As shown, the pressure rod 2032 is composed of two swing arms connected together. The connection between the two swing arms is rotatably connected to the base 2031 by a pin. A spring is connected between one swing arm and the base 2031, and a conductive protrusion 2032a is provided on the other swing arm.

[0059] To reduce friction between the conductive protrusion 2032a and the substrate 3012 array 301, and to improve the service life of the conductive protrusion 2032a, in an optional embodiment, the conductive protrusion 2032a can be a conductive copper ball, and the conductive copper ball is configured to be rollable. In this way, when the conductive protrusion 2032a comes into contact with the conductive sheet 3013 of the sample transport device 300, an electrical connection can be established between the conductive protrusion 2032a, the conductive sheet 3013, and the sample support sheet 3014.

[0060] Please continue to see as follows Figure 5 As shown, a concave guide rail 3015 is provided between two adjacent substrates 3012. In this way, since the concave guide rail 3015 can guide the conductive protrusion 2032a, it can make it easier for the conductive protrusion 2032a to be squeezed and engaged with the concave guide rail 3015, which can restrict the movement of the sample transport device 300.

[0061] In addition, after the conductivity measurement of the sample under test on the current substrate 3012, during the process of controlling the first linear module 302 to drive the substrate 3012 array 301 to move, the concave guide rail 3015 has a guiding effect on the conductive protrusion 2032a, which makes the first linear module 302 drive less effort in the initial stage, and there is no need to recalibrate when switching the next substrate 3012 to below the conductivity measuring device 202, which makes it much more convenient to perform conductivity measurement on a variety of samples under test.

[0062] Specifically, the reagent dispensing device 400 includes a dispensing gun 401 and a fourth linear module 402. The dispensing gun 401 is fixedly connected to the mounting bracket 600 via the fourth linear module 402. The movement trajectory of the fourth linear module 402 and the movement trajectory of the sample support sheet 3014 are on the same vertical plane.

[0063] To enable the drip gun 401 to move in three dimensions, the fourth linear module 402 includes an X-axis sub-linear module 4021 and a Z-axis sub-linear module 4022. Correspondingly, the drip gun 401 is fixedly connected to the X-axis sub-linear module 4021, and the X-axis sub-linear module 4021 is fixed to the Z-axis sub-linear module 4022.

[0064] Among them, the motion trajectory of the X-direction sub-linear module 4021 is consistent with the motion trajectory of the second linear module 201, and the motion trajectory of the Z-direction sub-linear module 4022 is on the same vertical plane as the motion trajectory of the first linear module 302.

[0065] In the process of measuring the conductivity of the sample to be tested, multiple reagents are required, necessitating the replacement of the dropper 401. Therefore, to facilitate the replacement of different droppers 401, the reagent dispensing device 400 may also include a dropper 401 array 403. The dropper array 403 is provided with multiple grids, each grid equipped with a dropper 401, which is pre-filled with the reagents required for the test.

[0066] It should be noted that, in order to further reduce the influence of external factors and improve the measurement accuracy of the single-molecule conductivity measurement system in this embodiment of the invention, the first linear module 302, the third linear module 2023 and the fourth linear module 402 can all be fixedly mounted on the vibration damping table 500.

[0067] To more easily understand the motion trajectories of the first linear module 302, the second linear module 201, the third linear module 2023, and the fourth linear module 402, the XYZ coordinate system is used as the reference coordinate system. For example, if the motion trajectory of the first linear module 302 is in the X direction, then the motion trajectory of the second linear module 201 is in the Y direction, the motion trajectory of the third linear module 2023 can be in the Z direction, and the motion trajectory of the fourth linear module 402 can also be in the Z direction.

[0068] Of course, in order to reduce the space occupied by the single-molecule conductivity measurement system in the embodiment of the present invention in the Z direction, the motion trajectory of the third linear module 2023 may have a certain angle with the motion trajectory of the first linear module 302, and the motion trajectory of the third linear module 2023 is perpendicular to the Y direction. Similarly, the motion trajectory of the fourth linear module 402 may have a certain angle with the motion trajectory of the sample support sheet 3014, and the motion trajectory of the fourth linear module 402 is perpendicular to the Y direction.

[0069] In some embodiments, the first linear module 302, the second linear module 201, the third linear module 2023, and the fourth linear module 402 can all be composed of a stepper motor, a rack, and a worm gear. The stepper motor drives the worm gear to rotate, and the linear movement of the rack is achieved by the meshing of the worm gear and the rack. Of course, the first linear module 302, the second linear module 201, the third linear module 2023, and the fourth linear module 402 can also be cylinders or hydraulic cylinders.

[0070] In addition, in order to clean the probe head 2022 of the conductivity meter 202 in a timely manner, such as Figure 2-4 As shown, a washing box 700 can also be provided for the conductivity measuring device 202. The washing box 700 is located on the central axis of the conductivity measuring device 202. The washing box 700 is filled with pre-made detergent, which can clean the probe head 2022 of the conductivity measuring device 202.

[0071] In practical applications, when measuring the conductivity of the sample under test, manual or automatic testing can be performed.

[0072] For manual testing, the following example will illustrate the process:

[0073] The reagents and the sample to be tested are manually added to the sample support 3014. The second linear module 201 is manually controlled to move directly above the sample, while the first linear module 302 can be switched to the sample to be tested. Then, the third linear module 2023 is manually controlled to adjust the distance between the probe 2022 of the conductivity meter 202 and the sample. Typically, the distance between the probe 2022 and the sample is a few millimeters. The conductivity meter 202 is then used to test the sample to obtain the conductivity measurement result.

[0074] Regarding automated testing, the following example illustrates the process:

[0075] The conductivity test program is started and run in the control device 100, and the control device 100 controls the first linear module 302, the second linear module 201, the third linear module 2023 and the fourth linear module 402 to return to the initial origin.

[0076] Then, the first linear module 302 is controlled to move along the Y-axis to the working area of ​​the reagent dispensing device 400. Subsequently, the fourth linear module 402 is controlled to move along the Z-axis and / or X-axis, so that the dispensing gun 401 reaches above the dispensing gun array 403 and automatically replaces the dispensing gun 401. Since the dispensing gun array 403 stores multiple dispensing guns 401, by controlling the movement of the first linear module 302, the dispensing gun 401 can dispense the reagents required for testing onto the samples to be tested on multiple substrates 3012.

[0077] After the reagent is added to the sample to be tested, the first linear module 302 is controlled to move to the bias mechanism 203, so that the conductive protrusion 2032a contacts the conductive sheet 3013 to achieve conductivity. By controlling the second linear module 201 and the third linear module 2023, the conductivity measuring device 202 is positioned directly above the sample to be tested, and the distance between the conductivity measuring device 202 and the sample to be tested is reduced by controlling the third linear module 2023.

[0078] When the probe 2022 of the conductivity measuring device 202 is only a few millimeters away from the first sample to be tested, the third linear module 2023 is controlled to stop moving, and then the conductivity measuring device 202 is used to test the sample. After obtaining a preset number of data points, the third linear module 2023 is controlled to move upward, and the first linear module 302 is controlled to send the second sample to be tested to the measurement point. The third linear module 2023 is then controlled to move downward again, so that the conductivity measuring device 202 can be used to test the sample.

[0079] The above operation can be repeated until all samples to be tested are completed, and then the first linear module 302 and the second linear module 201 are returned to their initial positions. The washing box 700 is positioned directly below the initial position of the third linear module 2023. By controlling the third linear module 2023 to move downward along the Z direction, the probe 2022 of the conductivity meter 202 is immersed in the washing box 700 for cleaning. After cleaning, the third linear module 2023 is controlled to move upward back to the initial position.

[0080] Secondly, through an embodiment of the present invention, the present invention provides a control method for a single-molecule conductivity measurement system, applied to the single-molecule conductivity measurement system described in the first aspect. Please refer to [example description]. Figure 7 As shown, the method may include the following steps:

[0081] S101: Control the sample transport device 300 to transport the sample to be tested to the working area of ​​the reagent dispensing device 400, and when the sample to be tested is detected to be in the working area of ​​the reagent dispensing device 400, control the reagent dispensing device 400 to dispense the target reagent into the sample to be tested.

[0082] S102: Control the transport device to transport the sample to be tested after the target reagent has been added to the working area of ​​the conductivity measuring device 200, and control the conductivity measuring device 200 to measure the conductivity of the sample to be tested.

[0083] The specific implementation methods of the single-molecule conductivity measurement system control method provided in this embodiment of the invention can be found in any of the implementation methods in the single-molecule conductivity measurement system described above. For the sake of brevity, they will not be described in detail here.

[0084] Thirdly, such as Figure 8 As shown, based on the same inventive concept, the present invention provides a readable storage medium 800 through an embodiment of the present invention, on which a program 801 is stored, which, when executed by a processor, implements any of the embodiments in the single-molecule conductivity measurement system control method described above.

[0085] The technical solutions in the above embodiments of the present invention have at least the following technical effects or advantages:

[0086] 1. Compared with the current traditional single-molecule conductivity instruments, the single-molecule conductivity measurement system provided in this embodiment of the invention uses multiple linear modules to realize functions such as reagent addition, probe head 2022 cleaning, automatic sample delivery, and automatic testing. No human control is required throughout the process, or the operator can remotely measure the conductivity of the sample to be tested through the control device 100. This effectively solves the technical problems of low automation and low measurement accuracy of existing single-molecule conductivity measurement instruments.

[0087] 2. Furthermore, unlike the traditional bias voltage application method in single-molecule conductivity measurement instruments, this embodiment of the invention provides a new bias voltage mechanism 203. Utilizing conductive protrusions 2032a, an electrical connection can be established between the conductive protrusions 2032a, the conductive sheet 3013, and the sample support sheet 3014, effectively replacing the process of manually tightening screws to secure the wires each time. Simultaneously, the substrate 3012 array 301 allows the conductive protrusions 2032a to slide freely within the concave guide rails 3015, significantly reducing the impact between the linear module's moving conductive protrusions 2032a and the sample support sheet 3014, thereby improving the service life of the bias voltage mechanism 203 and the sample support sheet 3014.

[0088] Those skilled in the art will understand that embodiments of the present invention can be provided as methods, systems, or computer products. Therefore, the present invention can take the form of a completely hardware embodiment, a completely software embodiment, or an embodiment combining software and hardware aspects. Furthermore, the present invention can take the form of a computer product embodied on one or more computer-usable storage media (including, but not limited to, disk storage, CD-ROM, optical storage, etc.) containing computer-usable code.

[0089] This invention is described with reference to flowchart illustrations and / or block diagrams of methods, apparatus (systems), and computer products according to embodiments of the invention. It will be understood that each block of the flowchart illustrations and / or block diagrams, and combinations of blocks in the flowchart illustrations and / or block diagrams, can be implemented by computer instructions. These computer instructions can be provided to a processor of a general-purpose computer, special-purpose computer, embedded processor, or other programmable data processing apparatus to produce a machine, such that the instructions, which execute via the processor of the computer or other programmable data processing apparatus, generate instructions for implementing the flowchart illustrations and / or block diagrams. Figure 1 One or more processes and / or boxes Figure 1 A device that provides the functions specified in one or more boxes.

[0090] These computer instructions may also be stored in a computer-readable storage medium that can direct a computer or other programmable data processing device to function in a particular manner, such that the instructions stored in the computer-readable storage medium produce an article of manufacture including instruction means, which are implemented in a process Figure 1 One or more processes and / or boxes Figure 1 The function specified in one or more boxes.

[0091] These computer instructions may also be loaded onto a computer or other programmable data processing equipment to cause a series of operational steps to be performed on the computer or other programmable equipment to produce a computer-implemented process, thereby providing instructions that execute on the computer or other programmable equipment for implementing the process. Figure 1 One or more processes and / or boxes Figure 1 The steps of the function specified in one or more boxes.

[0092] Although preferred embodiments of the invention have been described, those skilled in the art, upon learning the basic inventive concept, can make other changes and modifications to these embodiments. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments as well as all changes and modifications falling within the scope of the invention.

[0093] Obviously, those skilled in the art can make various modifications and variations to this invention without departing from its spirit and scope. Therefore, if these modifications and variations fall within the scope of the claims of this invention and their equivalents, this invention also intends to include these modifications and variations.

Claims

1. A single molecule conductance measurement system, characterized by, The single-molecule conductivity measurement system includes a control device and a conductivity measurement device, a sample transport device, and a reagent dispensing device electrically connected to the control device; wherein, the control device is used for: The sample transport device is controlled to transport the sample to be tested to the working area of ​​the reagent dispensing device, and when the sample to be tested is detected to be in the working area of ​​the reagent dispensing device, the reagent dispensing device is controlled to add the target reagent to the sample to be tested. The transport device is controlled to transport the sample to be tested after the target reagent has been added to it to the working area of ​​the conductivity measuring device, and the conductivity measuring device is controlled to measure the conductivity of the sample to be tested. The sample transport device includes: a substrate array, the substrate array including a substrate array base plate and a plurality of substrates disposed on the substrate array base plate; wherein, each substrate has a conductive sheet and a sample support sheet disposed on its upper surface, the sample support sheet being electrically connected to the conductive sheet; the sample support sheet is used to hold the sample to be tested; and a first linear module fixedly connected to the substrate array base plate, the first linear module reciprocating along a fixed direction. The conductivity measuring device includes: a second linear module with a fixed plate, the movement trajectory of the second linear module being perpendicular to the movement trajectory of the first linear module; and a conductivity measuring instrument fixedly connected to the fixed plate via a third linear module, the movement trajectory of the third linear module being on the same vertical plane as the movement trajectory of the first linear module. The conductivity measuring device includes: a piezoelectric sensor, the base of which is disposed on the third linear module and the signal output terminal of which is electrically connected to the control device; and a probe which is magnetically connected to the protective cover of the piezoelectric sensor. The conductivity measuring device further includes: a biasing mechanism, which consists of a base and a pressure rod; the base is fixedly connected to the second linear module; the pressure rod consists of two swing arms connected together, and the connection between the two swing arms is rotatably connected to the base via a pin; a spring is connected between one swing arm and the base, and a conductive protrusion is provided on the other swing arm; when the conductive protrusion is pressed into contact with the conductive sheet of the sample transport device, an electrical connection is established between the conductive protrusion, the conductive sheet, and the sample support sheet.

2. The system as described in claim 1, characterized in that, A concave guide rail is provided between two adjacent substrates; when the conductive protrusion is pressed against the concave guide rail, the conductive protrusion restricts the movement of the sample transport device.

3. The system as described in claim 2, characterized in that, The reagent adding device includes: The drip gun is fixedly connected to the mounting bracket via a fourth linear module; the movement trajectory of the fourth linear module and the movement trajectory of the sample support sheet are on the same vertical plane.

4. The system as described in claim 3, characterized in that, The fourth linear module includes an X-direction sub-linear module and a Z-direction sub-linear module; the drip gun is fixedly connected to the X-direction sub-linear module, and the X-direction sub-linear module is fixed to the Z-direction linear module; The motion trajectory of the X-direction sub-linear module is consistent with the motion trajectory of the second linear module, and the motion trajectory of the Z-direction sub-linear module is on the same vertical plane as the motion trajectory of the first linear module. The reagent dispensing device also includes a dispensing gun array, which has multiple grids, each grid being equipped with a dispensing gun.

5. A control method for a single-molecule conductivity measurement system, characterized in that, Applied to the single-molecule conductivity measurement system as described in any one of claims 1-4, the method comprises: The sample transport device is controlled to transport the sample to be tested to the working area of ​​the reagent dispensing device, and when the sample to be tested is detected to be in the working area of ​​the reagent dispensing device, the reagent dispensing device is controlled to add the target reagent to the sample to be tested. The transport device is controlled to transport the sample to be tested after the target reagent has been added to it to the working area of ​​the conductivity measuring device, and the conductivity measuring device is controlled to perform conductivity measurement on the sample to be tested.

6. A readable storage medium having a program stored thereon, characterized in that, When the program is executed by the processor, it implements the method of claim 5.

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