A pesticide composition and its application

Abstract

The present invention belongs to the technical field of pesticide killing insects and mites, and discloses a pesticide composition and its application, wherein the pesticide composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is nicofluprole, and the active ingredient B is any one of dimpropyridaz, fenmezoditiaz, indazapyroxamet or flometoquin. The mass ratio of the active ingredient A to the active ingredient B is 1:30 to 30:1. The pesticide composition of the present invention has excellent control effects on plant pests and mites, and is safe for crops.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide killing insects and mites, and specifically discloses a pesticide composition and application thereof. Background Art

[0002] Nicofluprole is a new type of phenylpyrazole nicotinamide fluorine-containing insecticide developed by Bayer. Its chemical name is N-methyl-N-cyclopropyl-2-chloro-5-[1-(2,6-dichloro-4-heptafluoroisopropylphenyl)-pyrazol-4-yl] nicotinamide. It has a broad-spectrum insecticidal activity and exhibits excellent insecticidal activity against lepidopteran pests, hemipteran pests and acarina pests. The chemical structure is:

[0003]

[0004] Dimpropyridaz is a pyrazole carboxamide insecticide developed by BASF. It has good control effects on aphids, whiteflies, thrips, leafhoppers and diamondback moths of Lepidoptera, Coleoptera, Diptera and Hemiptera. The insecticide is a racemic compound with a chiral center. Its chemical structure is novel and can be used for pest control of crops such as cotton, vegetables and fruits. Its chemical structure is:

[0005]

[0006] Fenmezoditiaz is a meso-ionic insecticide developed by BASF. It can effectively control piercing-sucking mouthparts pests such as Thysanoptera, Diptera and Hemiptera, and is particularly suitable for controlling Thysanoptera and Hemiptera pests. In addition, this type of compound can control internal and external parasites of animals. It has a low dosage, a long lasting effect, and is safe for animals. It is suitable for cereals, root crops, oil crops, vegetables, spices, and ornamental plants. Its chemical structure is:

[0007]

[0008] The chemical name of indazapyroxamet is N-(1-methylcyclopropyl)-2-(3-pyridyl)-2H-indazole-4-carboxamide, and its CAS registration number is 1689545-27-4. Its chemical structure is:

[0009]

[0010] Flometoquin is a novel quinoline insecticide jointly developed by Meiji Pharmaceutical Co., Ltd. and Nippon Kayaku Co., Ltd. It has excellent control effects on thysanoptera pests (thrips), whiteflies, gall mites and small lepidoptera pests, and has a fast killing speed and a long lasting effect. Flometoquin is an inhibitor of the complex III of the insect mitochondrial electron transport chain, acting on the complex IIIQi site, and its chemical structure is:

[0011]

[0012] In the actual process of agricultural production, the most difficult problem is the development of pesticide resistance. Long-term use of a single insecticide and acaricide for chemical control often accelerates the development and development of insecticide resistance in pests and mites. However, the development of new insecticides and acaricides is often difficult and time-consuming. The irrational use of insecticides and acaricides makes pests and mites develop resistance faster and faster. The development speed of new insecticides and acaricides is far behind the development of insecticide resistance in pests and mites. By rationally mixing existing pesticides, existing insecticide varieties can be protected and their service life of a single agent can be extended. Rationally mixing nicofluprole with any of dimpropyridaz, fenmezoditiaz, indazapyroxamet or flometoquin can not only extend the service life of a single agent. In addition, the inventors' compounding research has found that compounding nicofluprole with dimpropyridaz, fenmezoditiaz, indazapyroxamet or flometoquin can produce a good synergistic effect, and related reports on compounding nicofluprole with any of dimpropyridaz, fenmezoditiaz, indazapyroxamet or flometoquin have not been made public. Summary of the invention

[0013] Based on the above situation, the purpose of the present invention is to provide a pesticide composition, which has the characteristics of a broad insecticide and acaricide spectrum, a long lasting effect, significant synergistic effect, and safety for crops, and can significantly delay the development of pesticide resistance in pests and acarids and reduce the dosage of the agent.

[0014] In order to achieve the above object, the present invention adopts the following technical scheme: a pesticide composition and its application, the pesticide composition comprises an active ingredient A and an active ingredient B, the active ingredient A is nicofluprole, the active ingredient B is any one of dimpropyridaz, fenmezoditiaz, indazapyroxamet or flometoquin, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 30:1;

[0015] Furthermore, the active ingredient B is dimpropyridaz, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 25:1;

[0016] The active ingredient B is fenmezoditiaz, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 20:1;

[0017] The active ingredient B is indazapyroxamet, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 25:1;

[0018] The active ingredient B is flometoquin, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 20:1.

[0019] Furthermore, the active ingredient B is dimpropyridaz, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 25:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 20:1.

[0020] The active ingredient B is fenmezoditiaz, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 20:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 15:1;

[0021] The active ingredient B is indazapyroxamet, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 22:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 16:1;

[0022] The active ingredient B is flometoquin, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 20:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 15:1.

[0023] Furthermore, the total weight of the pesticide composition is 100wt%, and the total weight of the active ingredient A and the active ingredient B accounts for 1wt% to 70wt% of the total weight of the pesticide composition.

[0024] Furthermore, the pesticide composition can be prepared into a formulation form permitted in agriculture, and the formulation form is a solid formulation or a liquid formulation;

[0025] The liquid preparations include soluble solutions, soluble gels, oils, film-spreading oils, emulsifiable concentrates, latexes, dispersible solutions, ointments, aqueous emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspoemulsions, microcapsule suspension-suspensions, microcapsule suspension-water emulsions or microcapsule suspension-suspoemulsions;

[0026] The solid preparations include powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0027] Furthermore, the solid preparation is a wettable powder or a water-dispersible granule, and the liquid preparation is any one of a suspension, an emulsifiable concentrate, a dispersible oil suspension, an aqueous emulsion or a microemulsion;

[0028] Furthermore, the pesticide composition includes, in addition to the active ingredient, an auxiliary ingredient, wherein the auxiliary ingredient is selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist or a carrier;

[0029] Further, the wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, α-olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, saponin powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0030] The dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acid, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0031] The thickener is selected from one or more of xanthan gum, polyvinyl alcohol, organic bentonite, magnesium aluminum silicate, and carboxymethyl cellulose; and / or

[0032] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0033] The emulsifier is selected from one or more of fatty alcohol polyoxyethylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, phenylethylphenol polyoxyethylene polyoxypropylene ether, alkylphenol polyoxyethylene ether, fatty amine polyoxyethylene ether, alkylbenzene sulfonate, styrylphenol polyoxyethylene ether, fatty acid polyoxyethylene ester; and / or

[0034] The defoaming agent is selected from silicone oil, C 10 ~C 20 Saturated fatty acid compounds, C8~C 10 One or more of fatty alcohol compounds or silicone compounds; and / or

[0035] The preservative is selected from one or more of sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, and methyl p-hydroxybenzoate; and / or

[0036] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, epoxidized vegetable oil, and / or

[0037] The synergist is selected from synergist phosphite and synergist ether; and / or

[0038] The carrier is selected from one or more of kaolin, bentonite, attapulgite, light calcium carbonate, diatomaceous earth, and white carbon black; and / or

[0039] The solvent is selected from one or more of benzene, toluene, xylene, methanol, ethanol, isopropanol, n-butanol, diesel, N,N-dimethylformamide, cyclohexanone, ethyl acetate, N-methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, solvent oil, vegetable oil, vegetable oil derivatives and deionized water; and / or

[0040] The present invention also discloses the use of the above pesticide composition for preventing and controlling plant pests or plant mites;

[0041] Furthermore, the plant pests are lepidopteran pests, thysanoptera pests, and hemiptera pests;

[0042] Further, the lepidopteran pests include: Adoxophyes spp., Adoxophyes orana, Agrotis spp. (cutworm), Agrotis ipsilon (black cutworm), Alabama argillacea (cotton leafworm), Amorbia cuneana, Amyelosis transitella (navelorangeworm), Anacamptodes defectaria, Anarsia lineatella (peach twig borer), Anomis sabulifera (jute looper), Anticarsia g emma talis, Archips argyrospila (fruittreeleafroller), Archips rosana (rose leafroller), Agrotis spp. (black cutworm), Agrotis spp. (cotton leafworm), Amorbia cuneana, Amyelosis transitella (navelorangeworm), Anacamptodes defectaria, Anarsia lineatella (peach twig borer), Anomis sabulifera (jute looper), Anticarsia g emma talis, Archips argyrospila (fruittreeleafroller), Archips rosana (rose leafroller), Agrotis spp. (black cutworm ...grotis spp. (cotton leafworm), Agrotis spp. (cotton leafworm), Agrotis spp. (cotton leafworm), Agrotis spp. (cotton leafworm), Agrotis spp. leaf roller), Argyrotaenia spp. (tortricid moths), Argyrotaenia citrana (orange tortrix), Autographa gamma, Bonagota cranaodes, Borbo cinnara (rice leaf folder), Bucculatrix thurberiella (cotton leaf perforator), Caloptilia spp. (leaf miners), Capua reticulana, Carposina niponensis (peach fruit moth), Chilo spp., Chlumetia transversa (mango shoot borer), Choristoneura rosaceana (obliquebanded leafroller), Chrysodeixis spp.), Cnaphalocerus medinalis (grass leafroller), Colias spp., Conpomorpha cramerella, Cossus cossus (carpenter moth), Crambus spp. (sod webworms), Cydia funebrana (plum fruit moth), Cydia molesta (oriental fruit moth), Cydia nignicana (pea moth), Cydia pomonella (codling moth), Darna diducta, Diaphania spp. (stem borer), borers), Diatraea spp. (stalk borers), Diatraea spp. (sugarcane borer), Diatraea graniosella (southwester corn borer), Earias spp. (bollworms), Earias insulata (Egyptian bollworm), Earias vitella (rough northern bollworm), Ecdytopopha aurantianum, Elasmopalpus lignosellus (lesser cornstalk borer), Epiphysias postruttana (light brown apple moth), Ephestia spp. (flour moths), Ephestia cautella)(almond moth), Ephestia elutella(tobbacomoth), Ephestia kuehniella(Mediterranean flour moth), Epimeces spp.), Epinotia aporema, Erionotathrax (banana skipper), Eupoecilia ambiguella (grapeberry moth), Euxoa auxiliaris (army cutworm), Feltia spp. (cutworms), Gortyna spp. (stemblerers), Grapholitamolesta (oriental fruit moth), Hedylepta indicate (bean leaf webber), Helicoverpa sp p. (noctuids), Helicoverpa armigera, Helicoverpa zea, Heliothis spp., Heliothis budworm (Heliothis virescens), Hellula undalis (cabbage webworm), Indarbela spp. (root borers), Keiferia lycopersicella, Leucinodes orbonalis, Leucoptera malifoliella, Lithocollectis spp., Lobesia botrana (grape fruitmoth), Loxagrotis spp., Loxagrotis albicosta (western bean cutworm), Lymantria dispar (gypsy moth), Lyonetia clerkella (apple leaf miner), Mahasena corbetti (oil palm bagworm), bagworm) and Malacosoma spp.)(tent caterpillars), Mamestra brassicae(cabbage armyworm), Maruca testulalis(bean pod borer), Metisa plana, Mythimna unipuncta(true armyworm), Neoleucinodes elegantalis, Nymphula depunctalis, Operophtherabrumata, Ostrinia nubilalis(European corn borer), Oxydia vesulia, Pandemis cerasana(common currant tortrix), Pandemis heparana(brown apple tortrix), Papilio demodocus, Pectinophora gossypiella(pink bellworm), (bottomworm), Peridroma spp. (cutworm), Peridroma saucia (variegated cutworm), Perileucoptera coffeella (white coffee leafminer), Phthorimaea operculella (potato tuber moth), Phyllocnisitis citrella, Phyllonorycter spp., Pieris rapae, Plathypena scabra, Plodia interpunctella, Plutellaxylostella (diamondback moth), Polychrosis viteana (grapeberry moth), Prays endocarpa, Prays olive moth oleae) (olive moth), Pseudaletia spp.) (spot moth), Pseudaletia unipunctata (armyworm), Pseudoplusia includes (, Rachiplusia nu), Scirpophaga incertulas, Sesamia spp. (stem borers), Sesamia inferens (pink rice stem borer), Sesamia nonagrioides, Setora nitens, Sitotroga cerealella.

[0043] (Angoumois grain moth), Sparganothis pilleriana, Spodoptera spp., Spodoptera exigua, Spodoptera fugiperda, Spodoptera oridania (southern armyworm), Synanthedon spp., Thecla basilides, Thermisia gemmatalis, Tineola bisselliella (webbing clothes moth), Trichoplusiani (cabbage worm);

[0044] The Thysanoptera pests include: western flower thrips (Frankliniella occidentalis), thrips (Thrips spp.), Scirtothrips dorsalis;

[0045] The hemiptera pests include: Lygus spp., Eurygaster maura, Nezaraviridula, Piezodorus guildingi, Leptocorisa varicornis, Cimex lectularius, Cimex hemipterus, Aphis spp., Myzus Persicae, Rhopalosiphumspp., Dysaphis plantaginea, Toxopteraspp., Aphis craccivora Koch, Macrosiphum euphorbiae, Aulacorthum solani, Sitobion avenae, Metopolophium dirhodum, Schizaphis graminum), Brachycolus noxius, Nephotettix spp.), Nilaparvata lugens, Sogatella furcifera, Laodelphax striatellus, Aleurothrixus floccosus, Quadraspidiotus perniciosus, Unaspis yanonenses, Ceroplastes rubens, Aonidiella aurantii, Aleurocanthus spiniferus (Quaintance), Dialeurodes citri (Ashmead), Bemisa tabaci (Gennadius), Trialeurodes vaporariotum (Westwood), Dialeuroropora photiniana, Siphoninus phillyreae (Haliday), Aleurolobus marlatti (Quaintance), Parabemisia myricae (Kuwana), Aleuroclava psidii Singh, Aleuroclava guyavae (Takahashi), Aleuroclava aucubae (Kuwana), Crenidoresum turpiniae (Takahashi);

[0046] The harmful mites include: Tetranychidae, Eupodidae, Eriophyiade, Phytoseiidae, Acaridae, specifically including but not limited to Tetranychus spp., Panonychus spp., Tetranychus cinnabarinus, Eotetranychus carpini, Tetranychus truncatus, Tetranychus surticae Koch., Phyllocoptruta oleivora, Lycium barbarum gall mite, Red spider mite, Aculus pelekassi, Brevipalpus phoenicis, Boophilus spp., Dermacentor variabilis, Rhipicephalus sanguineus, Amblyomma americanum), true ticks (Ixodes spp.), cat anal mites (Notoedres cati), scabies mites (Sarcoptes scabiei), dust mites (Dermatophagoides spp.);

[0047] Furthermore, the plant pests are diamondback moth and aphids, and the plant harmful mites are gall mites.

[0048] The present invention has the following beneficial effects:

[0049] The pesticide composition of the present invention rationally compounds compounds with different action mechanisms, and its activity and insecticidal and acaricidal effects are not a simple superposition of the activities of each component, but have a significant synergistic effect, a wide insecticidal and acaricidal spectrum, good rapidity, a long lasting effect, and are safe for crops, meeting the safety requirements of pesticide preparations. DETAILED DESCRIPTION

[0050] The technical solutions in the embodiments of the present invention will be described clearly and completely below in combination with the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0051] Preparation Example:

[0052] Preparation Example 1: 24% nicofluprole·dimpropyridaz suspension (1:5)

[0053] Formula: 4% nicofluprole, 20% dimpropyridaz, 3% castor oil polyoxyethylene ether, 2% sodium lignin sulfonate, 3% sodium lauryl sulfate, 0.8% sodium p-hydroxybenzoate, 0.1% xanthan gum, 5% glycerol, 1% magnesium aluminum silicate, 0.02% silicone, and deionized water to make up the balance;

[0054] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added to mix evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0055] Preparation Example 2: 25% nicofluprole·dimpropyridaz wettable powder (3:2)

[0056] Formula: 15% nicofluprole, 10% dimpropyridaz, 2% sodium polycarboxylate, 4% alkylnaphthalene sulfonate formaldehyde condensate, 3% sodium lauryl sulfate, 3% white carbon black, 25% starch, kaolin makes up the balance;

[0057] Preparation method: according to the formula ratio of the embodiment, the active ingredient is added into the carrier, and the surfactant and other functional additives are added thereto, mixed, and then air flow pulverized and mixed again to obtain the wettable powder product.

[0058] Preparation Example 3: 26% nicofluprole·fenmezoditiaz emulsifiable concentrate (4:1)

[0059] Formula: 20.8% nicofluprole, 5.2% fenpyroximate, 20% propylene carbonate, 5% calcium dodecylbenzene sulfonate, 4% corn oil, 12% styrylphenol polyoxyethylene ether, and xylene to make up the balance;

[0060] Preparation method: according to the formula ratio of the embodiment, the active ingredients are added into the carrier, and the surfactant and other functional additives are added thereto, and the mixture is stirred and mixed evenly in a stirring mixing kettle to obtain an emulsifiable concentrate product.

[0061] Preparation Example 4: 32% nicofluprole·fenmezoditiaz water dispersible granules (1:3)

[0062] Formula: 8% nicofluprole, 24% fenmezoditiaz, 7% naphthalene sulfonate formaldehyde condensate, 3% sodium lignin sulfonate, 5% sodium dodecyl sulfonate, 12% ammonium sulfate, starch makes up the balance;

[0063] Preparation method: according to the formula ratio of the embodiment, the active ingredient is added to the carrier, and the surfactant and other functional additives are added thereto, mixed, and after air flow pulverization, 10-25% of water is added, and then kneading, granulation, drying, and sieving are performed to obtain a water-dispersible granule product; or the pulverized powder is sprayed with water in a boiling granulator, granulated, dried, and then sieved to obtain the product.

[0064] Preparation Example 5: 18% nicofluprole·indazapyroxamet suspension (1:8)

[0065] Formula: 2% nicofluprole, 16% indazapyroxamet, 1% sodium dodecyl sulfate, 1% naphthalenesulfonate formaldehyde condensate, 3% fatty alcohol polyoxyethylene ether phosphate, 0.2% xanthan gum, 1% magnesium aluminum silicate, 4% propylene glycol, 0.01% potassium benzisothiazolinone, 0.5% silicone oil, and deionized water to make up the balance;

[0066] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added to mix evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0067] Preparation Example 6: 18% nicofluprole·indazapyroxamet aqueous emulsion (1:2)

[0068] Formula: 6% nicofluprole, 12% indazapyroxamet, 12% trimethylbenzene, 10% cyclohexanone, 4% sodium dodecylbenzene sulfonate, 7% EO / PO block copolymer, 5% propylene glycol, 1% glycerol, 0.05% silicone defoamer, 0.1% xanthan gum, 0.1% sodium benzoate, and deionized water to make up the balance;

[0069] Preparation method: according to the formula ratio of the embodiment, the active ingredient is dissolved in the solvent and an emulsifier is added to dissolve it into a uniform oil phase, and deionized water and antifreeze are mixed together to form a uniform water phase; under high-speed shearing, the water phase is added to the oil phase to form a well-dispersed water emulsion product.

[0070] Preparation Example 7: 20% nicofluprole·flometoquin suspension (1:1)

[0071] Formula: 10% nicofluprole, 10% flometoquin, 1% isomeric tridecanol polyoxyethylene ether, 3% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 2% styrene phenol polyoxyethylene ether sulfate, 1% polycarboxylic acid sodium salt, 1.5% magnesium aluminum silicate, 0.1% carboxyethyl cellulose, 1% sodium sorbate, 5% ethylene glycol, 0.5% silicone oil, deionized water to make up the balance;

[0072] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added to mix evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0073] Preparation Example 8: 21% nicofluprole·flometoquin dispersible oil suspension (1:6)

[0074] Formula: 3% nicofluprole, 18% flometoquin, 3% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 10% polyoxyethylene sorbitan monooleate, 2% calcium dodecylbenzene sulfonate, 2% succinate sulfonate, 1% magnesium aluminum silicate, 1% Tesco 869, and corn oil makes up the balance;

[0075] Preparation method: According to the formula ratio of the preparation example, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, and vegetable oil is added and mixed evenly. After high-speed shearing, wet sand grinding, and finally homogenization filtration, a dispersible oil suspension product is obtained.

[0076] Preparation Example 9: 16% nicofluprole·flometoquin emulsifiable concentrate (1:3)

[0077] Formula: 4% nicofluprole, 12% flometoquin, 10% dimethyl sulfoxide, 12% styrylphenol polyoxyethylene ether, 2% calcium dodecylbenzene sulfonate, 20% propylene carbonate, and xylene makes up the balance;

[0078] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added to mix evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0079] Preparation Example 10: 30% nicofluprole·flometoquin water dispersible granules (4:1)

[0080] Formula: 24% nicofluprole, 6% flometoquin, 8% sodium lignin sulfonate, 3% naphthalene sulfonate formaldehyde condensate, 2.5% pull open powder BX, 1.5% sodium dodecylbenzene sulfonate, 10% ammonium sulfate, kaolin as the balance;

[0081] Preparation method: according to the formula ratio of the embodiment, the active ingredient is added to the carrier, and the surfactant and other functional additives are added thereto, mixed, and after air flow pulverization, 10-25% of water is added, and then kneading, granulation, drying, and sieving are performed to obtain a water-dispersible granule product; or the pulverized powder is sprayed with water in a boiling granulator, granulated, dried, and then sieved to obtain the product.

[0082] Example 1: Indoor bioactivity test of nicofluprole combined with dimpropyridaz, fenmezoditiaz, indazapyroxamet or flometoquin against Plutella xylostella

[0083] Reference test criteria: The test refers to NY / T 1154.14-2008 "Guidelines for Indoor Bioassay Tests of Pesticides Insecticides Part 14: Leaf Dipping Method" and NY / T 1154.7-2006 "Guidelines for Indoor Bioassay Tests of Pesticides Insecticides Part 7: Determination of Combined Action of Mixtures".

[0084] Experimental drugs: nicofluprole, dimpropyridaz, fenmezoditiaz, indazapyroxamet, and flometoquin original drugs.

[0085] Test subjects: Plutella xylostella larvae. The insect source is a sensitive strain that is continuously raised indoors. They are artificially raised for multiple generations in an intelligent insect breeding room, and healthy and consistent Plutella xylostella larvae are selected for use.

[0086] Preparation of medicine: Firstly, dissolve the original medicine with a suitable solvent to prepare a high-concentration mother solution, and then prepare the mixed solution with each single agent in a suitable proportion according to the activity of the medicine. Dilute the above single agent and the mixed solution into 5 series of mass concentrations with an aqueous solution containing 0.1% Tween 80.

[0087] Experimental treatment: Indoor bioassay adopts leaf dipping method. Select healthy and uniform 3rd instar larvae of diamondback moth for the test. Place the cabbage leaves in the test solution for 10 seconds, take them out, let them dry naturally and place them in a culture dish. Each dish is inoculated with 20 larvae. Each concentration treatment is repeated 4 times, and the solvent treatment without the same content of the drug is set as a blank control. Place them in an artificial climate chamber at (25±1)℃ and a photoperiod of 16h:8h (light:dark) for breeding.

[0088] Data investigation: 48 hours after the pesticide treatment, investigate the mortality of the test insects and record the number of dead insects and the total number of insects.

[0089] Data statistics and analysis:

[0090] According to the survey data, the adjusted mortality rate of each treatment was calculated according to formulas (1) and (2):

[0091]

[0092] Where:

[0093] P——mortality rate, in percentage (%);

[0094] K——Number of dead insects, in heads;

[0095] N——Total number of insects treated, in heads.

[0096]

[0097] Where:

[0098] P1——adjusted mortality rate, in percentage (%);

[0099] P t ——Treatment mortality rate, expressed as percentage (%);

[0100] P0——blank control mortality rate, in percentage (%).

[0101] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to formula (2); if the control mortality rate is greater than 20%, the test needs to be repeated.

[0102] The co-toxicity coefficient (CTC value) of the mixture is calculated according to formula (3), formula (4) and formula (5):

[0103]

[0104] Where:

[0105] ATI——Actual Toxicity Index of Mixture;

[0106] S——LC of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0107] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0108] TTI=TI A ×P A +TI B ×PB ···········(4)

[0109] Where:

[0110] TTI – Theoretical Toxicity Index of Mixtures;

[0111] TI A ——A: toxicity index of the agent;

[0112] P A ——The percentage of agent A in the mixture, in percentage (%);

[0113] TI B ——Toxicity index of agent B;

[0114] P B ——The percentage of agent B in the mixture, in percentage (%).

[0115]

[0116] Where:

[0117] CTC – Co-toxicity coefficient;

[0118] ATI——Actual Toxicity Index of Mixture;

[0119] TTI - Theoretical Toxicity Index of Mixture.

[0120] According to the logarithmic values ​​of each drug concentration and the corresponding corrected mortality probability value, the DPS statistical software was used to analyze and obtain the regression equation of each drug to calculate the LC 50 According to Sun Yunpei's calculation of the co-toxicity coefficient (CTC) of the mixture, the type of combined action of the mixture was evaluated (CTC < 80, there is an antagonistic effect between the individual doses of the mixture; CTC = 80-120, there is an additive effect between the individual doses of the mixture; CTC > 120, there is a synergistic effect between the individual doses of the mixture).

[0121] The indoor test results are shown in the following table:

[0122] Table 1 Results of indoor activity test of nicofluprole and dimpropyridaz against Plutella xylostella

[0123] Test drug Virulence regression equation <![CDATA[LC 50 (mg / L)]]> Co-toxicity coefficient nicofluprole y=6.2173+1.8468x 0.2192 / dimpropyridaz y=4.4712+1.7978x 1.9684 / 1:30 y=4.7847+1.8944x 1.2991 120.501 1:25 y=4.9351+1.6768x 1.0932 137.773 1:10 y=5.1698+1.7972x 0.8045 141.803 1:7 y=5.3412+1.7023x 0.6303 156.344 1:5 y=5.6314+1.9390x 0.4725 178.796 1:2 y=5.9119+1.8647x 0.3243 165.840 2:1 y=6.2917+1.8537x 0.2010 154.954 3:1 y=6.3186+1.8172x 0.1881 149.817 8:1 y=6.3458+1.8037x 0.1794 135.571 12:1 y=6.2453+1.6879x 0.1829 128.640 20:1 y=6.2367+1.7098x 0.1891 121.039

[0124] The results of the indoor biological activity assay test showed (Table 1) that the combination of nicofluprole and dimpropyridaz showed good biological activity against Plutella xylostella. The mass ratio of nicofluprole to dimpropyridaz was 1:30-20:1, and the co-toxicity coefficient was greater than 120, showing a synergistic effect. Among them, when the mass ratio of nicofluprole to dimpropyridaz was 1:5, the co-toxicity coefficient was the largest, and the synergistic effect was significant.

[0125] Table 2 Results of indoor activity test of nicofluprole and fenmezoditiaz against Plutella xylostella

[0126]

[0127]

[0128] The results of the indoor biological activity assay test showed (Table 2) that the combination of nicofluprole and fenmezoditiaz showed good biological activity against Plutella xylostella. The mass ratio of nicofluprole to fenmezoditiaz was 1:30-20:1, and the co-toxicity coefficient was greater than 120, showing a synergistic effect. Among them, when the mass ratio of nicofluprole to fenmezoditiaz was 1:3, the co-toxicity coefficient was the largest, and the synergistic effect was significant.

[0129] Table 3 Results of indoor activity test of nicofluprole and indazapyroxame against Plutella xylostella

[0130] Test drug Virulence regression equation <![CDATA[LC 50 (mg / L)]]> Co-toxicity coefficient nicofluprole y=6.2173+1.8468x 0.2192 / indazapyroxamet y=2.4062+1.9289x 22.1146 / 1:30 y=3.7808+1.9971x 4.0783 128.429 1:25 y=4.0908+1.7809x 3.2399 140.973 1:20 y=4.2512+1.9157x 2.4598 156.177 1:16 y=4.4540+1.8684x 1.9599 164.106 1:8 y=5.0267+1.8921x 0.9681 188.809 1:4 y=5.4100+1.8062x 0.5929 177.805 8:1 y=6.6366+2.0268x 0.1558 158.084 16:1 y=6.6158+1.9996x 0.1556 149.586 20:1 y=6.4928+1.9236x 0.1675 137.341 25:1 y=6.3959+1.8827x 0.1814 125.622 30:1 y=6.3083+1.8491x 0.1961 115.468

[0131] The results of the indoor biological activity assay test showed (Table 3) that the combination of nicofluprole and indazapyroxame showed good biological activity against Plutella xylostella. The mass ratio of nicofluprole to indazapyroxame was 1:30-25:1, and the co-toxicity coefficient was greater than 120, showing a synergistic effect. Among them, when the mass ratio of nicofluprole to indazapyroxame was 1:8, the co-toxicity coefficient was the largest, and the synergistic effect was significant.

[0132] Table 4 Results of indoor activity test of nicofluprole and flometoquin against Plutella xylostella

[0133] Test drug Virulence regression equation <![CDATA[LC 50 (mg / L)]]> Co-toxicity coefficient nicofluprole y=6.2173+1.8468x 0.2192 / flometoquin y=3.5810+1.6927x 6.8921 / 1:25 y=4.2188+2.0123x 2.4447 129.866 1:20 y=4.4345+1.8637x 2.0110 139.907 1:12 y=4.7163+1.8575x 1.4215 145.090 1:8 y=5.0383+1.6691x 0.9485 165.805 1:6 y=5.2756+1.8095x 0.7042 182.976 1:1 y=6.0501+1.7098x 0.2431 174.779 3:1 y=6.3057+1.7183x 0.1738 166.399 6:1 y=6.5117+1.9228x 0.1636 155.492 10:1 y=6.4301+1.7840x 0.1579 152.220 15:1 y=6.3153+1.7211x 0.1721 135.572 20:1 y=6.3021+1.7886x 0.1871 122.819

[0134] The results of the indoor biological activity assay test showed (Table 4) that the combination of nicofluprole and flometoquin showed good biological activity against Plutella xylostella. The mass ratio of nicofluprole to flometoquin was 1:25 to 20:1, and the co-toxicity coefficient was greater than 120, showing a synergistic effect.

[0135] Indoor bioassay tests (see Tables 1-4) showed that nicofluprole combined with any of dimpropyridaz, fenmezoditiaz, indazapyroxamet or flometoquin showed excellent biological activity against Plutella xylostella within a reasonable ratio.

[0136] Example 2: Indoor bioactivity test of nicofluprole combined with dimpropyridaz, fenmezoditiaz, indazapyroxamet or flometoquin against aphids

[0137] Reference test criteria: The test refers to NY / T 1154.14-2008 "Guidelines for Indoor Bioassay Tests of Pesticides Insecticides Part 14: Leaf Dipping Method" and NY / T 1154.7-2006 "Guidelines for Indoor Bioassay Tests of Pesticides Insecticides Part 7: Determination of Combined Action of Mixtures".

[0138] Experimental drugs: nicofluprole and dimpropyridaz, fenmezoditiaz, indazapyroxamet, and flometoquin original drugs.

[0139] Test target: peach aphid (Myzus persicae).

[0140] Drug preparation: First dissolve the original drug in a suitable solvent, then prepare a high-concentration mother liquor to prepare single-dose mother liquors, and design a reasonable ratio based on the purpose of mixing and the activity of the drug. Each single dose and each group of mixed doses are prepared in the required series of mass concentrations according to the equal ratio method.

[0141] Test method: Select fresh, insect-free and pesticide-free cabbage leaves that are basically consistent in size, leaf color and other indicators, wash them and make leaf discs. Immerse the leaf discs in the test solution, take them out after 10 seconds, and place them in an insect box covered with moisturizing filter paper.

[0142] Wingless adult aphids of the same size and vitality were selected and placed on the treated leaf discs. Each replicate was 30 heads. Each treatment with each concentration of the pesticide and the control treatment were repeated 4 times. The solvent treatment without the same concentration of the pesticide was set as a blank control. After the treatment, each insect box was placed in an artificial climate incubator with a temperature of (26±1)℃, a relative humidity of (60±5)%, and a light intensity of 16 hours for cultivation.

[0143] Experimental investigation: Observe the peach aphids 72 hours after treatment and record the number of insect deaths.

[0144] Data statistics and analysis:

[0145] According to the survey data, the adjusted mortality rate of each treatment was calculated according to formulas (1) and (2):

[0146]

[0147] Where:

[0148] P——mortality rate, in percentage (%);

[0149] K——Number of dead insects, in heads;

[0150] N——Total number of insects treated, in heads.

[0151]

[0152] Where:

[0153] P1——adjusted mortality rate, in percentage (%);

[0154] P t ——Treatment mortality rate, expressed as percentage (%);

[0155] P0——blank control mortality rate, in percentage (%).

[0156] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to formula (2); if the control mortality rate is greater than 20%, the test needs to be repeated.

[0157] The co-toxicity coefficient (CTC value) of the mixture is calculated according to formula (3), formula (4) and formula (5):

[0158]

[0159] Where:

[0160] ATI——Actual Toxicity Index of Mixture;

[0161] S——LC of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0162] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0163] TTI=TI A *P A +TI B *P B ···········(4)

[0164] Where:

[0165] TTI – Theoretical Toxicity Index of Mixtures;

[0166] TI A ——A: toxicity index of the agent;

[0167] P A ——The percentage of agent A in the mixture, in percentage (%);

[0168] TI B ——Toxicity index of agent B;

[0169] P B ——The percentage of agent B in the mixture, in percentage (%).

[0170]

[0171] Where:

[0172] CTC – Co-toxicity coefficient;

[0173] ATI——Actual Toxicity Index of Mixture;

[0174] TTI - Theoretical Toxicity Index of Mixture.

[0175] According to the logarithmic values ​​of each drug concentration and the corresponding corrected mortality probability value, the DPS statistical software was used to analyze and obtain the regression equation of each drug to calculate the LC 50 According to Sun Yunpei's calculation of the co-toxicity coefficient (CTC) of the mixture, the type of combined action of the mixture was evaluated (CTC < 80, there is an antagonistic effect between the individual doses of the mixture; CTC = 80-120, there is an additive effect between the individual doses of the mixture; CTC > 120, there is a synergistic effect between the individual doses of the mixture).

[0176] Table 5 Results of the indoor activity test of nicofluprole and dimpropyridaz against aphids

[0177]

[0178]

[0179] The results of the indoor biological activity assay test showed (Table 5) that the combination of nicofluprole and dimpropyridaz showed good biological activity against aphids. The mass ratio of nicofluprole to dimpropyridaz was 1:20-25:1, and the co-toxicity coefficient was greater than 120, showing a synergistic effect. Among them, the mass ratio of nicofluprole to dimpropyridaz was 3:1, the co-toxicity coefficient was the largest, and the synergistic effect was significant.

[0180] Table 6 Results of the indoor activity test of nicofluprole and fenmezoditiaz against aphids

[0181] Test drug Virulence regression equation <![CDATA[LC 50 (mg / L)]]> Co-toxicity coefficient nicofluprole y=4.2211+1.5375x 3.2106 / fenmezoditiaz y=5.3251+1.7854x 0.6575 / 1:30 y=5.4050+1.5375x 0.5452 123.773 1:25 y=5.4528+1.5233x 0.5044 134.466 1:15 y=5.5118+1.4801x 0.4510 153.412 1:8 y=5.5439+1.4640x 0.4251 169.660 1:4 y=5.5491+1.5189x 0.4350 179.735 4:1 y=5.0495+1.5683x 0.9299 194.338 8:1 y=4.8813+1.5336x 1.1950 187.691 10:1 y=4.8210+1.4845x 1.3201 179.755 15:1 y=4.7333+1.4587x 1.5235 169.582 20:1 y=4.6459+1.5651x 1.6836 160.940 30:1 y=4.5517+1.3957x 2.0953 136.172

[0182] The results of the indoor biological activity assay test showed (Table 6) that the combination of nicofluprole and fenmezoditiaz showed good biological activity against aphids. The mass ratio of nicofluprole to fenmezoditiaz was 1:30-30:1, and the co-toxicity coefficient was greater than 120, showing a synergistic effect. Among them, the mass ratio of nicofluprole to fenmezoditiaz was 4:1, the co-toxicity coefficient was the largest, and the synergistic effect was significant.

[0183] Table 7 Results of the indoor activity test of nicofluprole and indazapyroxamet against aphids

[0184]

[0185]

[0186] The results of the indoor biological activity assay test showed (Table 7) that the combination of nicofluprole and indazapyroxamet showed good biological activity against aphids. The mass ratio of nicofluprole to indazapyroxamet was 1:20-22:1, and the co-toxicity coefficient was greater than 120, showing a synergistic effect. Among them, the mass ratio of nicofluprole to indazapyroxamet was 1:2, the co-toxicity coefficient was the largest, and the synergistic effect was significant.

[0187] Table 8 Results of the indoor activity test on aphids by combining nicofluprole and flometoquin

[0188] Test drug Virulence regression equation <![CDATA[LC 50 (mg / L)]]> Co-toxicity coefficient nicofluprole y=4.2211+1.5375x 3.2106 / flometoquin y=4.4296+1.8021x 2.0727 / 1:25 y=4.6477+1.5024x 1.7159 122.463 1:20 y=4.6904+1.5286x 1.5943 132.239 1:14 y=4.7335+1.5192x 1.4976 141.751 1:7 y=4.7764+1.5760x 1.3865 156.421 1:3 y=4.8027+1.5785x 1.3335 170.544 1:1 y=4.7708+1.6222x 1.3845 181.951 3:1 y=4.7014+1.5131x 1.5753 179.212 7:1 y=4.6256+1.5180x 1.7646 170.261 10:1 y=4.5519+1.4944x 1.9946 153.313 15:1 y=4.5129+1.4365x 2.1832 142.181 20:1 y=4.4924+1.3749x 2.3397 133.727

[0189] The results of the indoor biological activity assay test showed (Table 8) that the combination of nicofluprole and flometoquin showed good biological activity against aphids. The mass ratio of nicofluprole to flometoquin was 1:25-20:1, and the co-toxicity coefficient was greater than 120, showing a synergistic effect. Among them, the mass ratio of nicofluprole to flometoquin was 1:1, the co-toxicity coefficient was the largest, and the synergistic effect was significant.

[0190] Example 3: Indoor bioactivity test of nicofluprole and flometoquin against Citrus rust tick

[0191] Test basis: The test refers to NY / T 1154.13-2008 "Guidelines for Indoor Bioassay Tests of Pesticides Insecticides Part 13: Leaf Disk Spray Method" and NY / T 1154.7-2008 "Guidelines for Indoor Bioassay Tests of Pesticides Insecticides Part 7: Determination of Combined Action of Mixtures".

[0192] Test target: Citrus rust mites (Phyllocoptruta oleivora), select adult mites with the same physiological state.

[0193] Test drugs: nicofluprole technical, flometoquin technical.

[0194] Instruments and equipment: microscope, hole puncher, sponge block, culture dish, Potter spray tower, constant temperature incubator.

[0195] Preparation of test materials: Select citrus leaves with uniform growth and without any pesticides, use a hole puncher to punch the citrus leaves into suitable leaf discs, place a sponge block in the culture dish to keep it moist, put filter paper on it, and place the citrus leaf disc on the filter paper, and inoculate the adult mites raised indoors onto the leaves, 30 heads per dish.

[0196] Preparation of medicine: Dissolve the original medicine in solvents separately, and then dilute with 0.1% Tween 80 aqueous solution. Prepare the mother liquor of single doses separately, and design a reasonable ratio according to the purpose of mixing and the activity of the medicine. Each single dose and each group of mixed doses are prepared according to the method of equal ratio to the required series of mass concentrations.

[0197] Chemical treatment: The spray pressure of the Potter spray tower is 1.47×10 5 To determine the stable state of Pa, the cleaned spray head was first cleaned twice with acetone and then cleaned twice with distilled water.

[0198] The culture dish was placed on the bottom plate of the Potter spray tower for spraying. The spray volume was 1 mL. The liquid was taken out after settling for 1 min and transferred to the breeding conditions for breeding.

[0199] Each treatment was repeated 4 times, and a treatment without drug was set as blank control.

[0200] Feeding and observation: The test insects of each treatment were placed under appropriate conditions for feeding and observation.

[0201] Data statistics and analysis: 48 hours after treatment, the mortality of test insects was checked and the total number of insects and the number of dead insects were recorded respectively.

[0202] According to the survey data, the adjusted mortality rate of each treatment was calculated according to formulas (1) and (2):

[0203]

[0204] Where:

[0205] P——mortality rate, in percentage (%);

[0206] K——indicates the number of dead insects, in units of heads;

[0207] N——represents the total number of insects treated, in heads.

[0208]

[0209] Where:

[0210] P1——adjusted mortality rate, in percentage (%);

[0211] P t ——Treatment mortality rate, expressed as percentage (%);

[0212] P0——blank control mortality rate, in percentage (%).

[0213] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to formula (2); if the control mortality rate is greater than 20%, the test needs to be repeated.

[0214] The DPS statistical analysis system was used to obtain the toxicity regression equation and LC 50 Value, to evaluate the activity of the test agent on the biological test material.

[0215] The co-toxicity coefficient (CTC value) of the mixture is calculated according to formula (3), formula (4) and formula (5):

[0216]

[0217] Where:

[0218] ATI——Actual Toxicity Index of Mixture;

[0219] S——LC of standard acaricide 50, the unit is milligrams per liter (mg / L);

[0220] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0221] TTI=TI A * P A +TI B * P B ·······(4)

[0222] Where:

[0223] TTI – Theoretical Toxicity Index of Mixtures;

[0224] TI A ——A: toxicity index of the agent;

[0225] P A ——The percentage of agent A in the mixture, in percentage (%);

[0226] TI B ——Toxicity index of agent B;

[0227] P B ——The percentage of agent B in the mixture, in percentage (%).

[0228]

[0229] Where:

[0230] CTC – Co-toxicity coefficient;

[0231] ATI——Actual Toxicity Index of Mixture;

[0232] TTI - Theoretical Toxicity Index of Mixture.

[0233] Table 9 Results of indoor activity test on nicofluprole and flometoquin combination against Citrus rust tick

[0234] Test drug Virulence regression equation <![CDATA[LC 50 (mg / L)]]> Co-toxicity coefficient nicofluprole y=4.1640+1.4567x 3.7490 / flometoquin y=4.7007+1.7355x 1.4875 / 1:30 y=4.8697+1.4949x 1.2222 124.122 1:20 y=4.9218+1.4758x 1.1298 135.554 1:12 y=4.9569+1.5064x 1.0681 146.043 1:6 y=4.9892+1.5069x 1.0166 160.119 1:3 y=5.0160+1.6337x 0.9777 179.162 1:1 y=4.8724+1.5362x 1.2107 175.924 4:1 y=4.6176+1.6430x 1.7090 168.218 8:1 y=4.5016+1.5592x 2.0876 153.632 15:1 y=4.4538+1.5126x 2.2967 149.069 20:1 y=4.3715+1.5163x 2.5972 134.603 30:1 y=4.2991+1.5373x 2.8569 125.091

[0235] The results of the indoor biological activity assay test showed (Table 9) that the combination of nicofluprole and flometoquin showed good biological activity against citrus rust mite. The mass ratio of nicofluprole to flometoquin was 1:30 to 30:1, and the co-toxicity coefficient was greater than 120, showing a synergistic effect. Among them, the mass ratio of nicofluprole to flometoquin was 1:3, the co-toxicity coefficient was the largest, and the synergistic effect was significant.

[0236] Field efficacy test

[0237] Example 4: Field efficacy test of compound preparation against Plutella xylostella

[0238] Test basis: The test refers to GB / T 17980.13-2000 "Guidelines for field efficacy tests of pesticides (I) Insecticides for controlling lepidopteran larvae of cruciferous vegetables".

[0239] Test target: Diamondback moth.

[0240] Test location: A field efficacy test was conducted in the cabbage fields of Liheng Village, Gaoguanzhai Town, Zhangqiu City, Shandong Province in April 2019.

[0241] Test method: When the pesticide was applied, the cabbage was in the vigorous growth period. The test set up 10 treatments (see Table 10), using random block arrangement, and the area of ​​the plot was 15m 2 Each treatment was repeated 4 times. The insect population was investigated before the application, and once at 3 and 14 days after the application, for a total of 3 times.

[0242] During the investigation, 5 points were randomly marked in each plot, and 2 plants were investigated at each point, for a total of 10 plants. The number of pests on the leaves of each cabbage plant was investigated, the number of live insects was counted, and the insect population reduction rate and control effect were calculated.

[0243]

[0244]

[0245] During the experiment, it was observed that the cabbage in each treatment plot grew well, and no phytotoxicity was observed in any treatment.

[0246] Table 10 Field efficacy test results of compound preparations against diamondback moth

[0247]

[0248] It can be seen from the field efficacy tests in the above table that the pesticide composition of the present invention exhibits good biological activity against Plutella xylostella and has a significant preventive effect, and can effectively control the damage and spread of Plutella xylostella.

[0249] Example 5: Field efficacy test of compound preparation against aphids

[0250] Test basis: The test refers to GB / T 17980.15-2000 "Guidelines for field efficacy tests of pesticides (I) Insecticides for the control of aphids in potato and other crops".

[0251] Test crops: Potatoes.

[0252] Test subjects: Mixed populations of green peach aphid and potato aphid.

[0253] The experiment was conducted at the Houjia Village Vegetable Planting Base in Tengzhou City, Zaozhuang City, Shandong Province. The experimental site is flat, with medium soil fertility and good irrigation conditions. The cultivation conditions of all experimental plots are uniform and consistent with local agricultural cultivation practices (GAP).

[0254] Experimental method: The experimental drug, control drug and blank control were randomly arranged in blocks, and protection rows were set between adjacent blocks. Each block had an area of ​​20m 2 , each repeated 4 times. The test was conducted on August 25, 2021, using the conventional spray method, with 45L of liquid applied per mu, and a total of one application.

[0255] Survey method: Investigate the base number of insect population before applying the medicine, and investigate the number of remaining insects 3 days and 7 days after applying the medicine, for a total of 3 surveys.

[0256] Five random sampling points were selected in each plot, and three plants were fixedly investigated at each point. Aphids on two leaves of each plant were marked, and the number of live insects was recorded.

[0257] Calculation method of drug efficacy: The drug efficacy is calculated according to the following formula:

[0258]

[0259]

[0260] During the experiment, potatoes in each treatment plot grew well and no phytotoxicity was observed in any treatment.

[0261] The test results are shown in the table below.

[0262] Table 11 Field efficacy test results of compound preparations against aphids

[0263]

[0264] From the field efficacy test in the table above, it can be seen that each compound preparation showed good biological activity against aphids. The control efficacy of the treatment groups of 25% nicofluprole·dimpropyridaz wettable powder (3:2), 26% nicofluprole·fenmezoditiaz emulsifiable concentrate (4:1), 18% nicofluprole·indazapyroxamet emulsion (1:2), and 20% nicofluprole·flometoquin suspension (1:1) was in the range of 85.35% to 91.14% 7 days after application, showing good control effect.

[0265] Example 6: Field efficacy test of the compound preparation against citrus rust tick

[0266] Test basis: The test refers to GB / T 17980.59-2004 "Guidelines for Field Efficacy Tests of Pesticides (II) Part 59: Acaricides for Control of Citrus Rust Mites".

[0267] Test location: Citrus plantation in Yinhe Village, Guansheng Town, Rongchang County, Chongqing. The test site has a deep soil layer and sandy loam. The citrus orchard has a flat terrain, good light conditions, and consistent fertilizer and water management levels.

[0268] Experimental crops: Navel orange (Newhall), 3-year-old trees.

[0269] Test target: Citrus rust tick (Phyllocoptruta oleivora Ashmead).

[0270] Experimental design: Two fruit trees were selected in each experimental plot, and each treatment was repeated 4 times. A 10x handheld magnifying glass was used to randomly sample leaves for investigation. When there were 3 to 5 live mites in each field of view on average, the pesticide was applied once. A Linong HD-400 backpack sprayer was used for uniform spraying. The base number of mites was investigated before application, and the number was investigated once 3d and 10d after application, and the number of live mites was recorded.

[0271] Survey method: In each plot, two trees were surveyed, and two damaged leaves of the same tip stage were selected from the east, west, south, north and center of the tree respectively. A total of 20 leaves were surveyed, and two fields of view were surveyed for each leaf to count the number of live mites.

[0272] Calculation method of protection effect:

[0273]

[0274]

[0275] The test results are shown in the following table:

[0276] Table 12 Field efficacy test results of compound preparations against citrus rust mites

[0277]

[0278] From the results of the field efficacy test in Table 12, it can be seen that the reasonable combination of nicofluprole and flometoquin can effectively control the damage of citrus rust mites. Three days after the application, the control effect of the combined preparation on citrus rust mites reached more than 81.64%, showing good rapid effectiveness; 10 days after the application, the control effect of 16% nicofluprole·flometoquin emulsifiable concentrate (1:3) on citrus rust mites was as high as 90.34%, with significant control effect.

[0279] It can be seen from the results of indoor bioassay tests and field control tests that the pesticide composition of the present invention exhibits good biological activity against Lepidoptera, Hemiptera, Acarina pests and mites, and is safe for crops.

[0280] The above describes the implementation modes of the present invention; however, the present invention is not limited to the above-mentioned implementation modes, and any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A pesticide composition, characterized in that: The pesticide composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is nicofluprole, the active ingredient B is dimpropyridaz, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 25:

1.

2. The pesticide composition according to claim 1, characterized in that The mass ratio of the active ingredient A to the active ingredient B is 1:25-20:

1.

3. The pesticide composition according to claim 1, characterized in that The total weight of the pesticide composition is 100wt%, and the total weight of the active ingredient A and the active ingredient B accounts for 1wt% to 70wt% of the total weight of the pesticide composition.

4. The pesticide composition according to claim 1, characterized in that The pesticide composition can be prepared into a formulation form permitted in agriculture, and the formulation form is a solid formulation or a liquid formulation.

5. The pesticide composition according to claim 4, characterized in that The solid preparation is a wettable powder or a water-dispersible granule, and the liquid preparation is any one of a suspension, an emulsifiable concentrate, a dispersible oil suspension, an aqueous emulsion or a microemulsion.

6. The pesticide composition according to claim 1, characterized in that In addition to the active ingredients, the pesticide composition also includes auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists or carriers.

7. Use of the pesticide composition according to any one of claims 1 to 6 for preventing and controlling Plutella xylostella and aphids.

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