A valve switching micro-fluidic chip

Abstract

The application is suitable for the field of in-vitro diagnosis and microfluidic detection technology, and provides a valve switching microfluidic chip, which comprises a valve, a microfluidic chip cover plate, a microfluidic chip flow channel layer and a microfluidic chip bottom plate; the valve comprises two groups of valve flow channel gating grooves; the valve is rotatably installed on one side of the microfluidic chip cover plate; the microfluidic chip flow channel layer comprises a reaction cavity, a reaction cavity flow channel, a special reagent reaction cavity flow channel and a plurality of reagent storage cavities; the lower end of each reagent storage cavity is provided with a storage cavity flow channel; one group of valve flow channel gating grooves are used for connecting the special reagent reaction cavity flow channel and the special reagent storage cavity flow channel; and the other group of valve flow channel gating grooves are used for connecting the reaction cavity flow channel and the general reagent storage cavity flow channel; therefore, the application has two independent channels which will not cross-infect, and the valve is integrated into the microfluidic chip to realize the functions of reagent storage, reagent mixing, reagent transfer, reagent reaction and reagent waste discharge.

Description

Technical Field

[0001] This invention belongs to the fields of in vitro diagnostics and microfluidic detection technology, and particularly relates to a rotary valve type microfluidic chip. Background Technology

[0002] Microfluidic chips can automatically detect results using only a small amount of time and sample. They are simple to operate, can be performed anytime and anywhere, and can automatically generate reports. Microfluidic chips fully meet the requirements of point-of-care testing (POCT), enabling more timely diagnosis and treatment for patients. A key development direction for POCT diagnostics is the miniaturization and integration of sampling, testing, calibration, and reporting into a single miniaturized, integrated laboratory-on-a-chip technology.

[0003] Microfluidic technology has the following advantages when applied to the field of in vitro diagnostics:

[0004] 1. Integration of miniaturization and automation

[0005] Microfluidics technology can integrate multiple steps of sample detection onto a small chip. By combining the size and curvature of the flow channels, microvalve and cavity design, these operations are integrated together, ultimately making the entire detection process miniaturized and automated.

[0006] 2. High throughput

[0007] Because microfluidics can be designed with multiple channels, a microchannel network can simultaneously distribute the sample to multiple reaction units. These reaction units are isolated from each other, preventing interference between reactions, and adjustments can be made as needed. Multiple tests can be performed on the same sample in parallel. Compared to traditional item-by-item testing, this significantly shortens testing time, improves efficiency, and offers high throughput.

[0008] 3. Low consumption of testing reagents

[0009] Due to the miniaturization of integrated detection, the reaction unit cavities on microfluidic chips are extremely small. Although the concentration of reagent formulations can be increased proportionally, the amount of reagent used is far lower than that of conventional reagents, significantly reducing the amount of reagent consumed.

[0010] 4. Small sample size requirement

[0011] Because the testing is performed entirely on a small chip, the sample size required is extremely small, often only microliters or even nanometers. Furthermore, whole blood can be used directly for testing, making it more convenient for infants, the elderly, people with disabilities, and other groups with low blood volume or difficult venous collection; or for very rare samples, making it possible to test multiple indicators.

[0012] 5. Less pollution

[0013] Due to the integrated functionality of microfluidic chips, all operations that previously required manual completion in the laboratory are now integrated into the chip and performed automatically, minimizing environmental contamination of samples during manual operations. For example, in molecular nucleic acid detection, both the sample itself and the nucleic acid prepared for testing can contaminate the laboratory, and the diffusion of aerosols can easily lead to false positives in subsequent sample testing.

[0014] However, existing rotary valve microfluidic chips generally only have one switching channel, which cannot separate the delivery of special solutions and general solutions during solution delivery, making it easy for cross-contamination to occur during the reaction process.

[0015] In conclusion, the existing technology obviously has inconveniences and defects in practical use, so it is necessary to improve it. Summary of the Invention

[0016] To address the aforementioned shortcomings, the present invention aims to provide a rotary valve-type microfluidic chip with two independent channels that prevent cross-contamination. Integrating this rotary valve into the microfluidic chip enables functions such as reagent storage, reagent mixing, reagent transfer, reagent reaction, and reagent waste disposal.

[0017] To achieve the above objectives, the present invention provides a rotary valve microfluidic chip, comprising a rotary valve, a rotary valve sealing gasket, a microfluidic chip cover plate, a microfluidic chip channel layer, a microfluidic chip base plate, a silicone pad, and a pressure plate; wherein the microfluidic chip cover plate, the microfluidic chip channel layer, and the microfluidic chip base plate are bonded and fixed together; the rotary valve includes two sets of rotary valve channel selection slots; the rotary valve is rotatably mounted on one side of the microfluidic chip cover plate; the microfluidic chip cover plate includes several sets of storage / reaction chamber cover plate connection holes; the microfluidic chip channel layer includes a reaction chamber and a reaction... The system includes a reaction chamber flow channel and a special reagent reaction chamber flow channel connected by a cavity, and several reagent storage cavities located at the upper end of the reaction chamber. Each reagent storage cavity has a flow channel layer reagent storage cavity vent at the upper end and a storage cavity flow channel at the lower end. The special reagent reaction chamber flow channel corresponds to the special reagent storage cavity flow channel, and the reaction chamber flow channel corresponds to the general reagent storage cavity flow channel. One set of the rotary valve flow channel selection slots is used to connect the special reagent reaction chamber flow channel and the special reagent storage cavity flow channel, while another set of the rotary valve flow channel selection slots is used to connect the reaction chamber flow channel and the general reagent storage cavity flow channel.

[0018] According to the rotary valve microfluidic chip of the present invention, a rotary valve sealing gasket is provided between the rotary valve and the microfluidic chip cover plate. The structure of the rotary valve sealing gasket is consistent with the bottom surface structure of the rotary valve and is installed accordingly, including a rotary valve sealing gasket indicator angle and a rotary valve sealing gasket selection groove.

[0019] According to the rotary valve microfluidic chip of the present invention, the microfluidic chip cover plate further includes a reagent storage chamber cover plate vent, a reagent storage chamber cover plate number, and a reagent storage chamber cover plate indicator area; wherein, the reagent storage chamber cover plate vent connects the chip reagent storage chamber to the atmosphere; the reagent storage chamber cover plate number marks the serial number of each reagent storage chamber, and the rotary valve indicator angle points to different reagent storage chamber cover plate indicator areas, thereby selecting different reagent storage chambers to connect to the reaction chamber, and different flow channels are switched through the storage / reaction chamber connection hole and the rotary valve flow channel selection groove; the upper end of the reaction chamber is connected to the flow channel layer reaction chamber vent.

[0020] According to the rotary valve microfluidic chip of the present invention, the microfluidic chip channel layer further includes a waste liquid chamber disposed at the lower end of the reaction chamber and communicating therewith; the waste liquid chamber is connected to the reaction chamber through a waste liquid chamber channel; the waste liquid chamber is connected to a waste liquid chamber vent in a channel layer.

[0021] According to the rotary valve microfluidic chip of the present invention, the silicone pad and the pressure plate have the same structure and are both provided with corresponding air hole structures.

[0022] According to the rotary valve microfluidic chip of the present invention, the silicone pad and the pressure plate have the same structure and are both provided with corresponding air hole structures.

[0023] According to the rotary valve microfluidic chip of the present invention, rotating the rotary valve and the rotary valve sealing gasket simultaneously allows the flow channel selection groove to select different reagent storage chambers and reaction chambers.

[0024] According to the rotary valve microfluidic chip of the present invention, the rotary valve is mounted on the microfluidic chip cover plate by a bolt structure; the rotary valve, the microfluidic chip cover plate, the microfluidic chip channel layer, the microfluidic chip base plate, the silicone pad and the pressure plate are all provided with through hole structures for bolt structure installation, including rotary valve through hole, chip cover plate through hole, channel layer through hole, base plate through hole, silicone pad through hole and pressure plate through hole.

[0025] This invention provides a rotary valve-type microfluidic chip, comprising a rotary valve, a rotary valve sealing gasket, a microfluidic chip cover plate, a microfluidic chip channel layer, a microfluidic chip base plate, a silicone pad, and a pressure plate; wherein the microfluidic chip cover plate, the microfluidic chip channel layer, and the microfluidic chip base plate are bonded and fixed together; the rotary valve includes two sets of rotary valve channel selection slots; the rotary valve is rotatably mounted on one side of the microfluidic chip cover plate; the microfluidic chip cover plate includes several sets of storage / reaction chamber cover plate connection holes; the microfluidic chip channel layer includes a reaction chamber and a spacer communicating with the reaction chamber. The invention includes a reaction chamber flow channel, a special reagent reaction chamber flow channel, and several reagent storage chambers located at the upper end of the reaction chamber. Each reagent storage chamber has a flow channel layer with reagent storage chamber vents at the upper end and a storage chamber flow channel at the lower end. The special reagent reaction chamber flow channel corresponds to the special reagent storage chamber flow channel, and the reaction chamber flow channel corresponds to the general reagent storage chamber flow channel. One set of rotary valve flow channel selection slots connects the special reagent reaction chamber flow channel and the special reagent storage chamber flow channel; while another set of rotary valve flow channel selection slots connects the reaction chamber flow channel and the general reagent storage chamber flow channel. This invention has two independent channels that prevent cross-contamination. Integrating this rotary valve into a microfluidic chip enables functions such as reagent storage, reagent mixing, reagent transfer, reagent reaction, and reagent waste disposal. Attached Figure Description

[0026] Figure 1 This is a schematic diagram of the overall chip structure;

[0027] Figure 2 This is an overview diagram of the overall chip structure;

[0028] Figure 3 This is a front view of the rotary valve;

[0029] Figure 4 This is a rear view of the rotary valve;

[0030] Figure 5 This is a structural diagram of the rotary valve sealing gasket;

[0031] Figure 6 This is a schematic diagram of the cover plate structure for a microfluidic chip.

[0032] Figure 7 This is a schematic diagram of the flow channel layer structure of a microfluidic chip;

[0033] Figure 8 This is a schematic diagram of the microfluidic chip substrate structure;

[0034] Figure 9 This is a schematic diagram of the silicone pad structure;

[0035] Figure 10 This is a front view of the pressure plate;

[0036] Figure 11This is a view of the back of the pressure plate;

[0037] Figure 12 This is a schematic diagram of the first working mode of the rotary valve;

[0038] Figure 13 This is a schematic diagram of the second working mode of the rotary valve;

[0039] Figure 14 This is a schematic diagram of the third working mode of the rotary valve;

[0040] Figure 15 This is a schematic diagram of the fourth working mode of the rotary valve;

[0041] Figure 16 This is a schematic diagram of the fifth working mode of the rotary valve;

[0042] Figure 17 This is a schematic diagram of the sixth working mode of the rotary valve;

[0043] In the diagram, 1 represents the rotary valve, 1-2 represents the rotary valve indicator angle, 1-3 represents the rotary valve through-hole, 1-4 represents the rotary valve flow channel selection groove, 2 represents the rotary valve gasket, 2-1 represents the rotary valve gasket indicator angle, 2-2 represents the rotary valve gasket selection groove, 3 represents the microfluidic chip cover plate, 3-1 represents the reagent storage chamber cover plate vent, 3-2 represents the reagent storage chamber cover plate number, 3-3 represents the reagent storage chamber cover plate indicator area, 3-4 represents the storage / reaction chamber cover plate connection hole, 3-5 represents the chip cover plate through-hole, 4 represents the microfluidic chip flow channel layer, 4-1 represents the flow channel layer reagent storage chamber vent, 4-2 represents the reagent storage chamber, 4-3 represents the special reagent storage chamber flow channel, and 4-4 represents the special reagent reaction chamber flow channel. 4-5 Through holes in the flow channel layer; 4-6 Flow channel in the general reagent storage chamber; 4-7 Flow channel in the reaction chamber; 4-8 Air vent in the waste liquid chamber of the flow channel layer; 4-9 Air vent in the reaction chamber of the flow channel layer; 4-10 Flow channel in the waste liquid chamber; 4-11 Reaction chamber; 4-12 Waste liquid chamber; 5 Microfluidic chip base plate; 5-1 Air vent in the reaction chamber of the base plate; 5-2 Through hole in the base plate; 5-3 Air vent in the waste liquid chamber of the base plate; 6 Silicone pad; 6-1 Air vent in the waste liquid chamber of the silicone pad; 6-2 Air vent in the reaction chamber of the silicone pad; 6-3 Through hole in the silicone pad; 7 Pressure plate; 7-1 Air vent in the waste liquid chamber of the pressure plate; 7-2 Air vent in the reaction chamber of the pressure plate; 7-3 Through hole in the pressure plate; 7-4 Hexagonal nut groove. Detailed Implementation

[0044] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0045] This is a schematic diagram of a rotary valve provided for some embodiments. The rotary valve has at least reagent storage and channel communication functions, and is used to connect at least one reagent storage chamber to another. This rotary valve is applicable at least to the clinical testing and in vitro diagnostic fields. This rotary valve can be used at least during experiments to connect a reagent reaction chamber to a reagent storage chamber. The rotary valve integrates two fluid switching channels that are not interconnected or cross-connected to avoid reagent contamination. The first fluid switching channel connects chambers 1 to 9 to the reaction chamber, and the second fluid switching channel connects chambers 10 and 11 to the reaction chamber.

[0046] See Figure 1 , Figures 12-17 To achieve reagent storage and flow channel switching, this invention designs as follows: Figure 2 The structure shown includes a rotary valve 1, a rotary valve sealing gasket 2, a microfluidic chip cover plate 3, a microfluidic chip channel layer 4, a microfluidic chip base plate 5, a silicone pad 6, and a pressure plate 7.

[0047] The microfluidic chip cover plate includes several sets of storage / reaction chamber cover plate connection holes;

[0048] See Figure 3 , Figure 4 and Figure 5 The rotary valve 1 includes a rotary valve indicator angle 1-2, a rotary valve through-hole 1-3, and a rotary valve flow channel selection groove 1-4. The rotary valve indicator angle 1-2 indicates the currently connected channel. The indicator number is located in the reagent storage chamber cover plate indicator area 3-3 on the microfluidic chip cover plate 3. Numbers 1 to 11 indicate that cavities 1 to 11 are connected to reaction chambers 4-11, and number 0 indicates no connection. The rotary valve is mounted on the microfluidic chip cover plate via bolts. The rotary valve through-hole 1-3, chip cover plate through-hole 3-5, flow channel layer through-hole 4-5, and base plate through-hole 5-2 (…) Figure 8 As shown), the silicone pad through-hole 6-3 and the pressure plate through-hole 7-3 are connected by screws that are pre-tightened to achieve the rotation and fixation of the rotary valve. A specified pre-tightening force can be applied using a torque wrench. The rotary valve flow channel selection groove 1-4 is the fluid switching channel when the flow channel is switched. The channel is connected and switched by connecting the storage / reaction chamber cover plate connection holes 3-4 of different groups.

[0049] The rotary valve gasket 2 includes a rotary valve gasket indicator angle 2-1 and a rotary valve gasket selector groove 2-2. The rotary valve gasket 2 functions to enable communication, rotation, and sealing between the rotary valve 1 and the microfluidic chip cover plate 3. The rotary valve gasket selector groove 2-2 and the rotary valve flow channel selector groove 1-4 combine to form a fluid switching channel.

[0050] See Figure 6The microfluidic chip cover plate 3 has an engraved reagent storage chamber cover plate number 3-2 and a reagent storage chamber cover plate indicator area 3-3. The reagent storage chamber cover plate number 3-2 identifies the reagent storage chamber 4-2. The reagent storage chamber cover plate indicator area 3-3 indicates the reagent storage chamber 4-2 currently connected to the reaction chamber 4-11.

[0051] See Figure 7 A set of storage / reaction chamber cover connection holes 3-4 connects one channel. The outer hole of the storage / reaction chamber cover connection hole 3-4 connects to the reagent storage chamber 4-2 through the general reagent storage chamber flow channel 4-6 or the special reagent storage chamber flow channel 4-3. The outer hole of the storage / reaction chamber cover connection hole 3-4 connects to the reaction chamber flow channel 4-7 and the special reagent reaction chamber flow channel 4-4 and the reaction chamber 4-11. The inner and outer holes of the storage / reaction chamber cover connection hole 3-4 are connected or closed through the rotary valve sealing gasket and the rotary valve.

[0052] The reaction chamber 4-11 is connected to the external drive pump through the flow channel layer reaction chamber vent 4-9, the bottom plate reaction chamber vent 5-1, the silicone pad reaction chamber vent 6-2, the pressure plate reaction chamber vent 7-2, and the external drive pump of the reaction chamber.

[0053] The waste liquid chamber is connected to the external drive pump through the waste liquid chamber vents 4-8 in the flow channel layer, the waste liquid chamber vents 5-3 in the bottom plate, the waste liquid chamber vents 6-1 in the silicone pad, the waste liquid chamber vents 7-1 in the pressure plate, and the waste liquid chamber itself.

[0054] The reagent storage chamber 4-2 is connected to the atmosphere through the reagent storage chamber vent 4-1 in the flow channel layer and the vent 3-1 in the reagent storage chamber cover plate.

[0055] Furthermore, by using a rotary valve to connect the reaction chamber 4-11 and the reagent storage chamber 4-2, the external drive pump of the waste liquid chamber is sealed. The external drive pump of the reaction chamber provides negative pressure to the reaction chamber. Under the action of pressure, the solution in the reagent storage chamber 4-2 is transferred to the reaction chamber through the channel. Under the action of the side wall of the chamber and gravity, the solution slides to the bottom of the reaction chamber.

[0056] Furthermore, the external driving pump of the reaction chamber applies negative and positive pressures of appropriate frequency and amplitude to drive the solution to reciprocate in the reagent storage chamber 4-2 to achieve solution mixing.

[0057] Furthermore, an external pump drives the sealed reaction chamber. The reaction chamber 4-11 is connected to the reagent storage chamber (where the solution has been pumped out) and the atmosphere via a rotary valve. An external pump drives the waste liquid chamber, applying negative pressure. Under this pressure, the solution in reaction chamber 4-11 is transferred to the waste liquid chamber through the waste liquid chamber channel 4-10.

[0058] Furthermore, the external drive pump of the waste liquid chamber applies negative and positive pressures of appropriate frequency and amplitude to drive the solution to reciprocate in the reaction chamber 4-11 to achieve solution mixing.

[0059] See Figures 9-11 The silicone pad 6 connects the pressure plate 7 and the microfluidic chip substrate 5. The silicone pad 6 and the pressure plate 7 can evenly distribute the force of the rotary valve pre-tightening screw to the microfluidic chip substrate 5 to achieve a better sealing effect. The hexagonal nut groove 7-4 machined on the pressure plate 7 can be used to fix the nut.

[0060] During the use of the microfluidic chip, the chip is placed in the designated position. The waste liquid chamber vent 7-1 of the pressure plate is connected to the external drive pump of the waste liquid chamber, ensuring communication between the waste liquid chamber and the external drive pump. The reaction chamber vent 7-2 of the pressure plate is also connected to the external drive pump of the reaction chamber, ensuring communication between the reaction chamber and the external drive pump. The rotary valve is connected to the rotary valve drive device, which can drive the rotary valve to rotate a specified number of steps, ensuring that the rotary valve rotates to the designated channel.

[0061] The rotary valve is initially in position 0 on the left, at which point there is no channel connecting it to the reaction chamber. Under the action of the drive device, the rotary valve rotates 15° clockwise, and the indicator angle 1-2 indicates position 1. At this point, reagent storage chamber 4-2 is connected to reaction chamber 4-11 via the general reagent storage chamber flow channel 4-6 and the reaction chamber flow channel 4-7. Only reagent storage chamber 1 is connected to the reaction chamber at this time; the other reaction chambers are not connected. The external drive pump for the waste liquid chamber is sealed. The external drive pump for the reaction chamber applies appropriate frequency and amplitude of negative and positive pressure to propel the solution in reagent storage chamber 4-2 in reciprocating motion, waiting for mixing to complete. When the external drive pump for the reaction chamber applies negative pressure, the liquid in reagent storage chamber 4-2 flows through reagent storage chamber 4-2, reaction chamber flow channel 4-7, rotary valve sealing gasket selection groove 2-2, rotary valve flow channel selection groove 1-4, and reaction chamber flow channel 4-7 into reaction chamber 4-11. The liquid in reagent storage chamber 1 is completely transferred to reaction chamber 4-11.

[0062] Furthermore, under the action of the rotary valve drive device, the rotary valve rotates 15° clockwise, and the rotary valve indicator angle 1-2 indicates position 2. At this time, only reagent storage chamber 4-2 (No. 2) is connected to the reaction chamber, while the other reaction chambers are not connected. The external drive pump of the waste liquid chamber is sealed, and the external drive pump of the reaction chamber applies negative pressure. Under the action of negative pressure, the liquid in reagent storage chamber 4-2 is completely transferred to reaction chamber 4-11.

[0063] Furthermore, the external drive pump of the reaction chamber is sealed, the reaction chamber 4-11 remains connected to the reagent storage chamber 2, and the reagent storage chamber 2 4-2 remains connected to the atmosphere. The external drive pump of the waste liquid chamber applies negative and positive pressures of appropriate frequency and amplitude to drive the solutions in the reagent storage chambers 1 and 2 to reciprocate in the flow channel 4-10 of the reaction chamber 4-11 to complete the mixing.

[0064] Furthermore, the external drive pump of the reaction chamber is sealed, and the external drive pump of the waste liquid chamber applies negative pressure. Under the action of negative pressure, the liquid in the reaction chamber 4-11 is completely transferred to the waste liquid chamber 4-12.

[0065] Furthermore, the rotary valve drive device can select reagent storage chambers 3 to 9 4-2 to complete the mixing and transfer of liquids in reagent storage chambers 3 to 9 4-2.

[0066] Furthermore, reagent storage chambers 10 (4-2) and 11 (4-2) are special reagent storage chambers. They are connected to reaction chamber 4-11 via special reagent storage chamber flow channel 4-3, independent rotary valve sealing gasket selection groove 2-2, independent rotary valve flow channel selection groove 1-4, and special reagent reaction chamber flow channel 4-4. Their connection channels are completely different from those of reaction chambers 1 to 9, avoiding cross-contamination. Their mixing and transfer principles are the same as those of reaction chambers 1 to 9.

[0067] Furthermore, after all reagent storage chambers 4-2 from 1 to 11 have been transferred, the rotary valve is rotated to the right-hand 0 position via the rotary valve drive device. At this time, reaction chamber 4-11 is disconnected from all reagent storage chambers 4-2.

[0068] This microfluidic chip can also perform reagent storage. Reagents are added to reagent storage chambers 1 through 11 (4-2), then the valve is set to the initial 0 position and pre-tightened to ensure the seal of the connection between reagent storage chamber 4-2 and the reaction chamber. Sealing tape is then applied to the vent holes 3-1 on all reagent storage chamber cover plates to ensure the seal of the connection between reagent storage chamber 4-2 and the atmosphere. At this point, reagent storage chamber 4-2 cuts off all channels connecting to the outside world, achieving airtight reagent storage.

[0069] In summary, this invention provides a rotary valve-type microfluidic chip, comprising a rotary valve, a rotary valve sealing gasket, a microfluidic chip cover plate, a microfluidic chip channel layer, a microfluidic chip base plate, a silicone pad, and a pressure plate; wherein the microfluidic chip cover plate, the microfluidic chip channel layer, and the microfluidic chip base plate are bonded together; the rotary valve includes two sets of rotary valve channel selection slots; the rotary valve is rotatably mounted on one side of the microfluidic chip cover plate; the microfluidic chip cover plate includes several sets of storage / reaction chamber cover plate connection holes; the microfluidic chip channel layer includes a reaction chamber and a reaction chamber... The invention comprises a connected reaction chamber flow channel and a special reagent reaction chamber flow channel, and several reagent storage chambers located at the upper end of the reaction chamber. Each reagent storage chamber has a flow channel layer reagent storage chamber vent at the upper end and a storage chamber flow channel at the lower end. The special reagent reaction chamber flow channel corresponds to the special reagent storage chamber flow channel; the reaction chamber flow channel corresponds to the general reagent storage chamber flow channel. One set of rotary valve flow channel selection slots connects the special reagent reaction chamber flow channel and the special reagent storage chamber flow channel; while another set of rotary valve flow channel selection slots connects the reaction chamber flow channel and the general reagent storage chamber flow channel. This invention has two independent channels that prevent cross-contamination. Integrating this rotary valve into a microfluidic chip enables functions such as reagent storage, reagent mixing, reagent transfer, reagent reaction, and reagent waste disposal.

[0070] Of course, the present invention may have other various embodiments. Without departing from the spirit and essence of the present invention, those skilled in the art can make various corresponding changes and modifications according to the present invention, but these corresponding changes and modifications should all fall within the protection scope of the appended claims.

Claims

1. A rotary valve type microfluidic chip, characterized in that, It includes a rotary valve, a rotary valve sealing gasket, a microfluidic chip cover plate, a microfluidic chip channel layer, a microfluidic chip base plate, a silicone pad, and a pressure plate; wherein the microfluidic chip cover plate, the microfluidic chip channel layer, and the microfluidic chip base plate are bonded and fixed together; The rotary valve includes two sets of rotary valve flow channel selection slots; the rotary valve is rotatably mounted on one side of the microfluidic chip cover plate; The microfluidic chip cover plate includes several sets of storage / reaction chamber cover plate connection holes; The microfluidic chip channel layer includes a reaction chamber, a reaction chamber channel communicating with the reaction chamber, a special reagent reaction chamber channel, and a plurality of reagent storage chambers located at the upper end of the reaction chamber; each reagent storage chamber has a channel layer reagent storage chamber vent at the upper end and a storage chamber channel at the lower end, wherein the special reagent reaction chamber channel corresponds to the special reagent storage chamber channel; the reaction chamber channel corresponds to the general reagent storage chamber channel; One set of the rotary valve flow channel selection slots is used to connect the special reagent reaction chamber flow channel and the special reagent storage chamber flow channel; while the other set of rotary valve flow channel selection slots is used to connect the reaction chamber flow channel and the general reagent storage chamber flow channel; The microfluidic chip cover plate also includes a reagent storage chamber cover plate vent, a reagent storage chamber cover plate number, and a reagent storage chamber cover plate indicator area; wherein, the reagent storage chamber cover plate vent connects the chip reagent storage chamber to the atmosphere; the reagent storage chamber cover plate number marks the serial number of each reagent storage chamber, and the indicator angle of the rotary valve points to different reagent storage chamber cover plate indicator areas, thereby selecting different reagent storage chambers to connect to the reaction chamber, and the switching of different flow channels is completed through the cooperation of the storage / reaction chamber connection hole and the flow channel selection groove of the rotary valve; The upper end of the reaction chamber is connected to the air vent of the flow channel layer reaction chamber.

2. The rotary valve microfluidic chip according to claim 1, characterized in that, A rotary valve sealing gasket is provided between the rotary valve and the microfluidic chip cover plate. The structure of the rotary valve sealing gasket is consistent with the bottom surface structure of the rotary valve and is installed accordingly. It includes a rotary valve sealing gasket indicator angle and a rotary valve sealing gasket selector groove.

3. The rotary valve microfluidic chip according to claim 1, characterized in that, The microfluidic chip channel layer also includes a waste liquid chamber located at the lower end of the reaction chamber and connected thereto; the waste liquid chamber is connected to the reaction chamber through a waste liquid chamber channel; the waste liquid chamber is connected to a waste liquid chamber vent in a channel layer.

4. The rotary valve microfluidic chip according to claim 3, characterized in that, The microfluidic chip substrate includes substrate reaction chamber pores that communicate with the pores of the flow channel layer reaction chamber and substrate waste liquid chamber pores that communicate with the pores of the flow channel layer waste liquid chamber.

5. The rotary valve microfluidic chip according to claim 1, characterized in that, The silicone pad and the pressure plate have the same structure, both with corresponding air pore structures.

6. The rotary valve microfluidic chip according to claim 2, characterized in that, By rotating the rotary valve and the rotary valve sealing gasket simultaneously, the flow channel selection groove can select between different reagent storage chambers and reaction chambers.

7. The rotary valve microfluidic chip according to claim 6, characterized in that, The rotary valve is mounted on the microfluidic chip cover plate by bolts. The rotary valve, microfluidic chip cover plate, microfluidic chip channel layer, microfluidic chip base plate, silicone pad and pressure plate are all provided with through holes for bolt mounting, including through holes for the rotary valve, chip cover plate, channel layer, base plate, silicone pad and pressure plate.

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