Composition for enhancing mitochondrial energy and application thereof

Abstract

The present invention provides a composition for enhancing mitochondrial energy and its use. The composition comprises adenosine, sodium mannose phosphate, and mannose. The components of the composition are rationally formulated and exhibit synergistic effects. The composition not only provides substances and energy for biochemical processes but also enhances the skin's antioxidant capacity, maintains cellular homeostasis, promotes collagen synthesis, and significantly improves skin aging.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to a composition for enhancing mitochondrial energy and an application thereof. Background Art

[0002] Aging is an inevitable natural process in life, generally believed to be the result of multiple factors, including exogenous environmental stimuli and endogenous genetic decay. The primary manifestation of endogenous genetic decay is the progressive accumulation of AGEs, which cause collagen to form molecular crosslinks. This not only reduces skin elasticity and permeability, diminishing the diffusion of nutrients and waste, but also reduces collagen solubility, making it difficult for collagenase to hydrolyze it. This leads to decreased skin elasticity, persistent wrinkles that deepen and become more difficult to smooth, thus accelerating the aging process. Consequently, skincare products that leverage the metabolism of skin cells are gaining increasing popularity.

[0003] CN108245450A discloses a compound containing pomegranate peel extract and its application. This compound includes pomegranate peel extract and glacier water, with the ratio of pomegranate peel extract to glacier water being 2 to 100 g / L. The disclosed compound has the ability to scavenge free radicals and enhance cellular autophagy, thereby enhancing the skin's antioxidant capacity, removing metabolic waste from the skin, thereby delaying aging and whitening the skin. It can be used as a functional additive in cosmetics.

[0004] CN113413398A discloses a fish egg extract and its application. The provided freshwater and marine fish egg extracts, both in their original form and in their various components, exhibit DPPH free radical scavenging activity, demonstrating a certain degree of in vitro antioxidant activity. The fish egg extract can inhibit the proliferation of MCF7 breast cancer cells and induce apoptosis, as evidenced by morphological changes and Annexin V / PI expression. Treatment of the cells with the fish egg extract for 24 hours revealed the production of reactive oxygen species and superoxide, as well as mitochondrial depolarization, in MCF7 cells.

[0005] However, these products do not consider the barrier repair and immune enhancement of aging and fragile skin, and cannot achieve an ideal health state. Based on this, it is necessary to develop a skin care product with multiple functions to meet the needs of modern people. Summary of the Invention

[0006] In view of the shortcomings of the prior art, the present invention aims to provide a composition for enhancing mitochondrial energy and its application.

[0007] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a composition for enhancing mitochondrial energy, wherein the composition for enhancing mitochondrial energy comprises adenosine, sodium mannose phosphate and mannose.

[0009] The adenosine in the composition provided by the present invention is an important intermediate for synthesizing adenosine triphosphate (ATP), and ATP can improve the body's metabolic capacity and slow down aging; the sodium mannose phosphate is the key to initiating lysosomal autophagy, and can be used to promote the differentiation of stem cells derived from human fat; the combination of mannose, adenosine, and sodium mannose phosphate can promote the penetration of effective ingredients through the skin barrier into deep skin tissues, increase the entry of effective ingredients into mitochondria, thereby enhancing mitochondrial energy, improving cell autophagy capacity, and promoting the synthesis of collagen and elastin. The three work together to have a synergistic effect, which can not only provide substances and energy for biochemical processes, but also improve the skin's own antioxidant capacity, maintain cell homeostasis, promote collagen synthesis, and significantly improve skin aging.

[0010] Preferably, the mass ratio of adenosine, sodium mannose phosphate and mannose is (3-5):(1-3):(1-2).

[0011] Preferably, the composition for enhancing mitochondrial energy further comprises creatine and / or yeast extract.

[0012] Preferably, the composition for enhancing mitochondrial energy comprises adenosine, sodium mannose phosphate, mannose, creatine and yeast extract.

[0013] As a preferred technical solution of the present invention, the creatine can store and transfer energy for skin cells and is an important source of skin energy. It can stimulate the biological vitality of skin cells through the transformation of DNA syntheses and DNA repair in skin cells, thereby delaying skin aging. The yeast extract contains a variety of small molecule amino acids and polysaccharides, which have the advantages of rich nutrition, small molecular weight, easy absorption, and comprehensive efficacy. It can specifically support the skin's own protection and has multiple functions such as delaying skin aging, moisturizing, improving skin texture, firming and removing wrinkles. The composition that enhances mitochondrial energy can further improve its effect of enhancing cell energy and delaying skin aging by adding creatine and yeast extract.

[0014] Preferably, the mass ratio of adenosine, sodium mannose phosphate, mannose, creatine and yeast extract is (3-5):(1-3):(1-2):(3-5):(0.1-1).

[0015] The first "3-5" can be 3.2, 3.4, 3.5, 3.7, 3.9, 4, 4.2, 4.5, 4.7 or 4.9, etc.;

[0016] "1-3" can be 1.2, 1.4, 1.5, 1.6, 1.8, 2, 2.2, 2.4, 2.5, 2.6 or 2.8, etc.;

[0017] "1-2" can be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9, etc.;

[0018] The second "3-5" can be 3.2, 3.4, 3.5, 3.7, 3.9, 4, 4.2, 4.5, 4.7 or 4.9, etc.;

[0019] “0.1-1” can be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8 or 0.9, etc.

[0020] Specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0021] In a second aspect, the present invention provides a use of the composition for enhancing mitochondrial energy as described in the first aspect in cosmetics.

[0022] Preferably, the cosmetics include essence cream, lotion, emulsion, facial cream or facial mask.

[0023] Preferably, the mass percentage of the composition for enhancing mitochondrial energy in the cosmetic is 1-15%, for example, it can be 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13% or 14%, etc. Specific values ​​within the above numerical ranges can be selected and will not be detailed here.

[0024] In a third aspect, the present invention provides a Ningyan anti-wrinkle essence cream, which comprises the composition for enhancing mitochondrial energy as described in the first aspect, a moisturizer, a thickener, a skin conditioner, an antioxidant, an emulsifier, a skin feel regulator and deionized water.

[0025] The Ningyan anti-wrinkle essence cream of the present invention can provide more nutrition and energy to skin cells through the combination of the composition that enhances mitochondrial energy with components such as moisturizers, antioxidants, and skin conditioners, thereby having a better effect of delaying skin aging, and can also better resist glycation, deeply moisturize the skin, and tighten the skin.

[0026] Preferably, the Ningyan anti-wrinkle essence cream comprises, by weight, 1-15 parts of the composition for enhancing mitochondrial energy as described in the first aspect, 1-20 parts of a moisturizer, 0.1-1 parts of a thickener, 5-10 parts of a skin conditioner, 0.1-1 parts of an antioxidant, 1-10 parts of an emulsifier, 1-10 parts of a skin feel regulator, and 1-90 parts of deionized water.

[0027] The amount of the composition for enhancing mitochondrial energy in the Ningyan anti-wrinkle cream of the present invention can be 2 parts, 4 parts, 5 parts, 6 parts, 8 parts, 10 parts, 12 parts or 14 parts, etc.;

[0028] The amount of the moisturizer added can be 1 part, 3 parts, 5 parts, 7 parts, 9 parts, 10 parts, 12 parts, 15 parts, 17 parts or 19 parts, etc.;

[0029] The amount of the thickener added can be 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts or 0.9 parts, etc.;

[0030] The amount of the skin conditioner added can be 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts or 9.5 parts, etc.;

[0031] The amount of the antioxidant added can be 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts or 0.9 parts, etc.;

[0032] The amount of the emulsifier added can be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts or 9 parts, etc.;

[0033] The amount of the skin feel regulator added can be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts or 9 parts, etc.;

[0034] The amount of deionized water added can be 5 parts, 10 parts, 20 parts, 30 parts, 40 parts, 50 parts, 60 parts, 70 parts, 80 parts or 90 parts, etc.

[0035] Preferably, the moisturizing agent includes any one or a combination of at least two of glycerin, butylene glycol, panthenol, hyaluronic acid, sodium hyaluronate, acetylated sodium hyaluronate, dipropylene glycol or Tremella polysaccharide.

[0036] Preferably, the moisturizing agent is a combination of butylene glycol, dipropylene glycol, hyaluronic acid, acetylated sodium hyaluronate and tremella polysaccharide.

[0037] In the present invention, when the moisturizing agent is a combination of butylene glycol, dipropylene glycol, hyaluronic acid, acetylated sodium hyaluronate and tremella polysaccharide, the moisturizing effect of the essence cream can be further improved, and butylene glycol, dipropylene glycol, hyaluronic acid, acetylated sodium hyaluronate and tremella polysaccharide also have a certain synergistic effect on the above-mentioned effects.

[0038] Preferably, the thickener comprises any one or a combination of at least two of acrylic acid (esters) / C10-30 alkyl acrylate crosspolymer, cetyl alcohol or stearyl alcohol.

[0039] Preferably, the antioxidant includes any one or a combination of at least two of retinyl palmitate, p-hydroxyacetophenone or tocopheryl acetate.

[0040] Preferably, the emulsifier includes any one or a combination of at least two of hydrogenated lecithin, phytosterols, C14-22 alcohol / C12-20 alkyl glucoside or ammonium acryloyldimethyltaurate / VP copolymer.

[0041] Preferably, the skin feel regulator includes any one or a combination of at least two of sodium polyacryloyldimethyl taurate, polyisobutene, PEG-7 trimethylolpropyl coco ether, cetyl ethylhexanoate, diisostearyl malate, polydimethylsiloxane, rice (ORYZASATIVA) germ oil, aluminum starch octenylsuccinate, squalane or jojoba seed oil.

[0042] Preferably, the skin conditioning agent includes any one of soluble collagen, lactobacillus / rice fermentation product, dipalmitoyl hydroxyproline, dipotassium glycyrrhizate, niacinamide, β-glucan, hydroxypropyl tetrahydropyrantriol, palmitoyl tripeptide-5, yeast lysate, citrus (CITRUS RETICULATA) peel extract, chlorella (CHLORELLA VULGARIS) / white lupin (LUPINUS ALBUS) protein fermentation product, hydrolyzed royal jelly protein, tetradecylaminobutyryl valinamidobutyric acid urea trifluoroacetate, soluble collagen, saffron extract or sweet orange extract, or a combination of at least two thereof.

[0043] Preferably, the skin conditioning agent is a combination of niacinamide, lactobacillus / rice fermentation product, dipalmitoylhydroxyproline, hydroxypropyl tetrahydropyrantriol, palmitoyl tripeptide-5, soluble collagen and tetradecylaminobutyryl valinamidobutyric acid urea trifluoroacetate.

[0044] In the present invention, when the skin conditioner is a combination of niacinamide, lactobacillus / rice fermentation product, dipalmitoyl hydroxyproline, hydroxypropyl tetrahydropyrantriol, palmitoyl tripeptide-5, soluble collagen and tetradecylaminobutyryl valinamidobutyric acid urea trifluoroacetate, the antioxidant and anti-glycation effects of the essence cream can be further enhanced.

[0045] Preferably, the anti-wrinkle essence cream further comprises any one of a pH regulator, a preservative or a fragrance, or a combination of at least two of them.

[0046] In a fourth aspect, the present invention provides a method for preparing the Ningyan anti-wrinkle essence cream according to the third aspect, the preparation method comprising:

[0047] (1) Add deionized water, moisturizer, and thickener to the water phase pot, stir and heat until warm; add emulsifier, skin feel regulator, and antioxidant to the oil phase pot, stir and heat until warm;

[0048] (2) After the heat preservation is completed, the water and oil pots are pumped into the emulsifying pot, homogenized, stirred and cooled under vacuum, and the composition for enhancing mitochondrial energy and the skin conditioner as described in the first aspect are added and mixed evenly to obtain the product.

[0049] Preferably, the insulation temperature in step (1) is independently 80-85°C (for example, 81°C, 82°C, 83°C, 84°C or 85°C, etc.), and the insulation time is independently 10-20 min (11 min, 13 min, 15 min, 17 min or 19 min, etc.).

[0050] Preferably, the homogenization temperature in step (2) is 60-70°C (for example, 61°C, 62°C, 63°C, 64°C, 65°C, 66°C, 67°C, 68°C or 69°C, etc.), the homogenization time is 5-10 min (for example, 6 min, 7 min, 8 min, 9 min or 10 min, etc.), and the homogenization speed is 500-1000 rpm (for example, 600 rpm, 700 rpm, 800 rpm, 900 rpm or 1000 rpm, etc.).

[0051] Preferably, the temperature in step (2) is lowered to 30-40°C (for example, 31°C, 32°C, 33°C, 34°C, 35°C, 36°C, 37°C, 38°C or 39°C, etc.).

[0052] Preferably, step (2) further comprises adding any one or a combination of at least two of a pH regulator, a fragrance or a preservative.

[0053] Specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0054] Compared with the prior art, the present invention has the following beneficial effects:

[0055] (1) The components of the composition for enhancing mitochondrial energy of the present invention are reasonably matched and have a synergistic effect. They can not only provide substances and energy for biochemical processes, but also improve the skin's own antioxidant capacity, maintain cell homeostasis, promote collagen synthesis, and significantly improve skin aging.

[0056] (2) The Ningyan anti-wrinkle essence cream of the present invention can provide more nutrition and energy to skin cells through the combination of the composition that enhances mitochondrial energy with components such as moisturizers, antioxidants, and skin conditioners, thereby having a better effect of delaying skin aging, and can also better resist glycation, deeply moisturize the skin, and tighten the skin. DETAILED DESCRIPTION

[0057] In order to further illustrate the technical means and effects adopted by the present invention, the technical solutions of the present invention are further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0058] The adenosine described in the examples of the present invention was purchased from Nanjing Spike Biotechnology Co., Ltd.

[0059] The sodium mannose phosphate was purchased from Beijing Yanzhishan Technology Co., Ltd.

[0060] The mannose was purchased from Beijing Yanzhishan Technology Co., Ltd.

[0061] The creatine was purchased from AlzChem Trostberg GmbH;

[0062] The yeast extract was purchased from Angel Yeast Co., Ltd.

[0063] The rest of the raw materials can be used as long as they are purchased from regular dealers.

[0064] The weight percentages of adenosine, sodium mannose phosphate, mannose, creatine, and yeast extract mentioned below are all calculated based on the actual content of the active ingredients contained in the commercially available raw materials. The commercially available raw materials may also optionally contain any one or a combination of at least two of solvents, fillers, diluents, stabilizers, pH adjusters, antibacterial agents, antioxidants, or impurities within the permitted range.

[0065] Example 1

[0066] This embodiment provides a composition for enhancing mitochondrial energy, comprising 2 parts of adenosine, 1 part of sodium mannose phosphate, 0.75 parts of mannose, 2 parts of creatine, and 0.25 parts of yeast extract.

[0067] Example 2

[0068] This embodiment provides a composition for enhancing mitochondrial energy, which comprises 2.5 parts of adenosine, 0.5 parts of sodium mannose phosphate, 1 part of mannose, 1.5 parts of creatine and 0.5 parts of yeast extract.

[0069] Example 3

[0070] This embodiment provides a composition for enhancing mitochondrial energy, comprising 1.5 parts of adenosine, 1.5 parts of sodium mannose phosphate, 0.5 parts of mannose, 2.5 parts of creatine, and 0.2 parts of yeast extract.

[0071] Example 4

[0072] This example provides a composition for enhancing mitochondrial energy. The composition for enhancing mitochondrial energy differs from Example 1 only in that creatine is not included, and its reduced amount is added to the yeast extract, while the other components and addition amounts remain unchanged.

[0073] Example 5

[0074] This example provides a composition for enhancing mitochondrial energy. The composition for enhancing mitochondrial energy differs from Example 1 only in that yeast extract is not included, and its reduced amount is added to creatine, while the other components and addition amounts remain unchanged.

[0075] Example 6

[0076] This embodiment provides a composition for enhancing mitochondrial energy, which comprises 3 parts of adenosine, 0.3 parts of sodium mannose phosphate, 1.1 parts of mannose, 1 part of creatine, and 0.6 parts of yeast extract.

[0077] Example 7

[0078] This embodiment provides a composition for enhancing mitochondrial energy, comprising 1 part of adenosine, 1.6 parts of sodium mannose phosphate, 0.4 part of mannose, 3 parts of creatine, and 0.04 part of yeast extract.

[0079] Comparative Example 1

[0080] This comparative example provides a composition for enhancing mitochondrial energy. The composition for enhancing mitochondrial energy differs from Example 1 only in that adenosine is not included, and the reduced amount thereof is distributed to sodium mannose phosphate and mannose in proportion to the number of parts, while the other components and the addition amounts remain unchanged.

[0081] Comparative Example 2

[0082] This comparative example provides a composition for enhancing mitochondrial energy. The composition for enhancing mitochondrial energy differs from Example 1 only in that sodium mannose phosphate is not included, and its reduced amount is distributed to adenosine and mannose in proportion to the number of parts, while the other components and addition amounts remain unchanged.

[0083] Comparative Example 3

[0084] This comparative example provides a composition for enhancing mitochondrial energy. The composition for enhancing mitochondrial energy differs from Example 1 only in that mannose is not included, and the reduced amount thereof is distributed to adenosine and sodium mannose phosphate in proportion to the number of parts, while the other components and the addition amounts remain unchanged.

[0085] Application Example 1

[0086] This application example prepares a condensed face anti-wrinkle cream, the formula of which is as follows:

[0087]

[0088] Its preparation method is:

[0089] (1) Add deionized water, moisturizer, skin conditioner, and thickener to the water phase pot, stir and heat to 85°C and keep warm for 45 minutes; add emulsifier, skin feel regulator, and antioxidant to the oil phase pot, stir and heat to 80°C and keep warm for 45 minutes;

[0090] (2) After the heat preservation is completed, the water and oil pots are pumped into the emulsification pot, homogenized at 65°C and 800 rpm for 20 minutes, cooled to 35°C, and the composition for enhancing mitochondrial energy as described in Example 1 is added and mixed evenly to obtain the product.

[0091] Application Example 2-7

[0092] This application example provides six types of anti-wrinkle essence creams. The only difference between their formulas and those of Application Example 1 is that the mitochondrial energy enhancing composition of Example 1 is replaced by the mitochondrial energy enhancing compositions of Examples 2-7 with the same addition amount. Other conditions remain unchanged. The preparation method refers to Application Example 1.

[0093] Application Example 8

[0094] This application example provides a rejuvenating essence cream. The formula differs from that of Application Example 1 only in that the moisturizer does not contain butylene glycol. The reduced amount of butylene glycol is distributed proportionally among dipropylene glycol, hyaluronic acid, sodium acetylated hyaluronate, and Tremella polysaccharide. The other ingredients and their contents remain unchanged. The preparation method is similar to that of Application Example 1.

[0095] Application Example 9

[0096] This application example provides a rejuvenating essence cream. The formula differs from that of Application Example 1 only in that the moisturizer does not contain dipropylene glycol. Its reduced amount is distributed proportionally among butylene glycol, hyaluronic acid, sodium acetylated hyaluronate, and Tremella polysaccharide. The other ingredients and their contents remain unchanged. The preparation method is similar to that of Application Example 1.

[0097] Application Example 10

[0098] This application example provides a rejuvenating essence cream. Its formula differs from that of Application Example 1 only in that the moisturizer does not include hyaluronic acid. Its reduced amount is distributed proportionally among butylene glycol, dipropylene glycol, sodium acetylated hyaluronate, and Tremella polysaccharide. The other ingredients and their contents remain unchanged. The preparation method is similar to that of Application Example 1.

[0099] Application Example 11

[0100] This application example provides a rejuvenating essence cream. The formula differs from that of Application Example 1 only in that the moisturizer does not include sodium acetylated hyaluronate. Its reduced amount is distributed proportionally among butylene glycol, dipropylene glycol, hyaluronic acid, and Tremella polysaccharide. The other ingredients and their contents remain unchanged. The preparation method is similar to that of Application Example 1.

[0101] Application Example 12

[0102] This application example provides a rejuvenating essence cream. The formula differs from that of Application Example 1 only in that the moisturizer does not contain Tremella fuciformis polysaccharide. Its reduced amount is distributed proportionally among butylene glycol, dipropylene glycol, hyaluronic acid, and sodium acetylated hyaluronate. The other ingredients and their contents remain unchanged. The preparation method is similar to that of Application Example 1.

[0103] Application Example 13

[0104] This application example provides a rejuvenating anti-wrinkle cream. Its formula differs from that of Application Example 1 only in that the skin conditioning agent does not include niacinamide. Its reduced amount is distributed proportionally among the lactobacillus / rice fermentation product, dipalmitoylhydroxyproline, hydroxypropyl tetrahydropyrantriol, palmitoyl tripeptide-5, soluble collagen, and tetradecylaminobutyryl valinamidobutyric acid urea trifluoroacetate. The other ingredients and their contents remain unchanged. The preparation method is similar to that of Application Example 1.

[0105] Application Example 14

[0106] This application example provides a rejuvenating anti-wrinkle cream. The formula differs from that of Application Example 1 only in that the skin conditioning agent does not contain Lactobacillus / rice fermentation product. Its reduced amount is distributed proportionally among niacinamide, dipalmitoylhydroxyproline, hydroxypropyl tetrahydropyrantriol, palmitoyl tripeptide-5, soluble collagen, and tetradecylaminobutyrylvalinamidobutyric acid urea trifluoroacetate. The other ingredients and their contents remain unchanged. The preparation method is similar to that of Application Example 1.

[0107] Application Example 15

[0108] This application example provides a rejuvenating anti-wrinkle cream. The formula differs from that of Application Example 1 only in that the skin conditioning agent does not contain dipalmitoylhydroxyproline. Its reduced amount is distributed proportionally among niacinamide, Lactobacillus / rice fermentation product, hydroxypropyl tetrahydropyrantriol, palmitoyl tripeptide-5, soluble collagen, and tetradecylaminobutyryl valinamidobutyric acid urea trifluoroacetate. The other ingredients and their contents remain unchanged. The preparation method is similar to that of Application Example 1.

[0109] Application Example 16

[0110] This application example provides a rejuvenating anti-wrinkle cream. The formula differs from that of Application Example 1 only in that the skin conditioning agent does not contain hydroxypropyl tetrahydropyrantriol. Its reduced amount is distributed proportionally among niacinamide, Lactobacillus / rice fermentation product, dipalmitoyl hydroxyproline, palmitoyl tripeptide-5, soluble collagen, and tetradecylaminobutyryl valinamidobutyric acid urea trifluoroacetate. The other ingredients and their contents remain unchanged. The preparation method is similar to that of Application Example 1.

[0111] Application Example 17

[0112] This application example provides a rejuvenating anti-wrinkle cream. The formula differs from that of Application Example 1 only in that the skin conditioning agent does not include palmitoyl tripeptide-5. Its reduced amount is distributed proportionally among niacinamide, lactobacillus / rice fermentation product, dipalmitoyl hydroxyproline, hydroxypropyl tetrahydropyrantriol, soluble collagen, and tetradecylaminobutyryl valinamidobutyric acid urea trifluoroacetate. The other ingredients and their contents remain unchanged. The preparation method is similar to that of Application Example 1.

[0113] Application Example 18

[0114] This application example provides a rejuvenating anti-wrinkle cream. The formula differs from that of Application Example 1 only in that the skin conditioning agent does not include soluble collagen. Its reduced amount is distributed proportionally among niacinamide, Lactobacillus / rice fermentation product, dipalmitoylhydroxyproline, hydroxypropyl tetrahydropyrantriol, palmitoyl tripeptide-5, and tetradecylaminobutyryl valinamidobutyric acid urea trifluoroacetate. The other ingredients and their contents remain unchanged. The preparation method is similar to that of Application Example 1.

[0115] Application Example 19

[0116] This application example provides a rejuvenating anti-wrinkle cream. The formula differs from that of Application Example 1 only in that the skin conditioning agent, tetradecylaminobutyrylvalinamidobutyryl urea trifluoroacetate, is omitted. Its reduced amount is distributed proportionally among niacinamide, lactobacillus / rice fermentation product, dipalmitoylhydroxyproline, hydroxypropyl tetrahydropyrantriol, palmitoyl tripeptide-5, and soluble collagen. The other ingredients and their contents remain unchanged. The preparation method is similar to that of Application Example 1.

[0117] Comparative Application Examples 1-3

[0118] Comparative Application Examples 1-3 provide three types of anti-wrinkle essence creams. The formulas of these creams differ from those of Application Example 1 only in that the mitochondrial energy-enhancing composition of Example 1 is replaced with the mitochondrial energy-enhancing compositions of Comparative Examples 1-3, added in the same amounts, respectively. All other ingredients and amounts remain unchanged. The preparation methods are similar to those of Application Example 1.

[0119] Comparative Application Example 4

[0120] This comparative application example provides an essence cream, the formula of which differs from that of Application Example 1 only in that it does not contain the composition for enhancing mitochondrial energy of Example 1, while the other ingredients and their contents remain unchanged. The preparation method is similar to that of Application Example 1.

[0121] Test Example 1

[0122] Experiment on promoting elastin production

[0123] The experiment of promoting elastin production was conducted using the Ningyan anti-wrinkle essence cream provided in Example 1-7 and the essence cream provided in Comparative Example 1-4. The method is as follows:

[0124] (1) Human fibroblasts were seeded on a 96-well plate at a density of 20,000 cells per well, culture medium was added, and the cells were cultured in a 37°C incubator for 24 hours. After the cells adhered to the wall, new culture medium was replaced, and each test sample was added (the final concentration of the control sample was 50 mg / mL). A 20 mmol / L PBS solution was used as a blank control, and the cells were cultured in a 37°C incubator for 2 days.

[0125] (2) After the culture is completed, the cells are washed with cell lysis buffer and ultrasonically disrupted. The content of elastin is detected using the Biocolor elastin detection kit: the sample is treated with an elastin precipitant to precipitate the elastin, centrifuged, and the supernatant is removed. The dye and reaction solution are added to the precipitate to react to form an elastin-dye complex, centrifuged and washed three times with PBS solution to remove unbound dye, and a dye releaser is added to release the dye from the elastin-dye complex. The absorbance of the sample at 513 nm is measured using a spectrophotometer, and the expression level of elastin is calculated based on the absorbance-dye concentration standard curve (the expression level of the blank group is set as 1);

[0126] The specific test results are shown in Table 1 below:

[0127] Table 1

[0128] Test samples Elastin expression Test samples Elastin expression Application Example 1 5.9 Application Example 7 5.0 Application Example 2 5.6 Comparative Application Example 1 3.5 Application Example 3 5.8 Comparative Application Example 2 2.7 Application Example 4 4.5 Comparative Application Example 3 3.8 Application Example 5 4.3 Comparative Application Example 4 2.0 Application Example 6 5.2

[0129] As can be seen from the test data in Table 1, the elastin expression levels of the anti-wrinkle creams provided in Application Examples 1-3 of the present invention are above 5.5, while the elastin expression level of the cream in Comparative Application Example 4 is only 2.0. This indicates that the addition of the mitochondrial energy-enhancing composition to the cream of the present invention can stimulate cell growth, increase skin elasticity, and thus improve skin condition. The elastin expression levels of the creams described in Comparative Application Examples 1-3 are only 2.7-3.8, which fully demonstrates that adenosine, sodium mannose phosphate, and mannose in the mitochondrial energy-enhancing composition cooperate with each other to achieve a synergistic effect in promoting elastin expression. As can be seen from Application Examples 4 and 5, the lack of either creatine or yeast extract in the mitochondrial energy-enhancing composition can also have a negative impact on the cream's ability to promote elastin expression.

[0130] Test Example 2 Antioxidant and Anti-glycation Test

[0131] (1) Take 1 g of the essence cream of Application Example 1-19 and Comparative Application Example 1-4, add 2 mL of anhydrous ethanol, and after complete dissolution, add 2 mL of 60 μmol / L DPPH solution and mix well. Let it stand for 30 minutes, adjust to zero with anhydrous ethanol, and measure the absorbance at 517 nm as Ai; in the same way, take 2 mL of anhydrous ethanol solvent + 2 mL of DPPH solution and mix well to measure the absorbance as Ac; take another 1 g of the composition matrix and dissolve it, add 2 mL of anhydrous ethanol, and then add 2 mL of anhydrous ethanol solvent and mix well to measure the absorbance as Aj.

[0132] The free radical scavenging rate was calculated according to the following formula.

[0133] Clearance (%) = [1-(Ai-Aj) / Ac] × 100%

[0134] Among them, Aj is the contribution of the reaction sample itself to the absorbance; Ai is the absorbance value of the sample after the action of DPPH; Ac is the absorption of DPPH itself at the measurement wavelength.

[0135] The DPPH free radical scavenging rate reflects its antioxidant capacity to a certain extent. A higher scavenging rate indicates a better antioxidant capacity and a better anti-aging effect. Therefore, the anti-aging effect of a composition can be determined by studying its DPPH free radical scavenging ability. The results are shown in Table 2 (each sample group was tested in parallel 5 times, and the data are presented as the average value).

[0136] (2) Anti-glycation Effect Test Based on the Fluorescence of AGEs: The inhibitory effect of the essence creams of Application Examples 1-13 and Comparative Examples 1-4 on AGE formation was evaluated using a glucose-β-Lg simulation system. Based on the fluorescence of AGEs, the fluorescence intensity of fluorescent AGEs in the blank control group and the test group with the composition added after 7 days of reaction was measured using an F-4600 fluorescence spectrometer at an excitation wavelength of 325 nm and an emission wavelength of 440 nm. The inhibition rate of fluorescent AGEs was calculated (each test sample was diluted to 5.0 wt% with phosphate buffer) as follows:

[0137] Inhibition rate (%) = [(F0-F1) / F0] × 100%;

[0138] Wherein, F0 is the fluorescence intensity of the blank control group; F1 is the fluorescence intensity of the experimental group;

[0139] The specific test results are shown in Table 2:

[0140] Table 2

[0141]

[0142]

[0143] As shown in Table 2, the anti-wrinkle cream prepared in Application Examples 1-3 has a good scavenging effect on DPPH, with a scavenging rate of over 86%, and an inhibition rate of over 91% on fluorescent AGEs, indicating that the various ingredients in the cream of the present invention cooperate with each other, synergistically enhance their effectiveness, have a good antioxidant effect, can help reverse cell glycation, and reduce glycation reactions. As shown in Application Examples 13-19, the combination of skin conditioners in the cream of the present invention affects the antioxidant and anti-glycation effects of the cream. As shown in Comparative Example Application Examples 1-3, adenosine, sodium mannose phosphate, and mannose in the composition for enhancing mitochondrial energy cooperate with each other to significantly enhance the antioxidant and anti-glycation effects of the cream.

[0144] Test Example 3

[0145] Anti-wrinkle effect experiment

[0146] The anti-wrinkle effect experiment was conducted using the Ningyan anti-wrinkle essence cream provided in Example 1-7 and the essence cream provided in Comparative Example 1-4. The specific test method is as follows:

[0147] (1) Subjects: 110 female volunteers aged 35-55 years with healthy skin were randomly divided into 11 groups, with half male and half female.

[0148] (2) Experimental instrument: Skin wrinkle tester VL650 from German CK company;

[0149] (3) Test method: A skin wrinkle tester VL650 from German CK company was used and a silicone sleeve was used to measure the wrinkle depth on the forehead of the subject. The forehead wrinkles of the subjects were tested separately and the average value of the test data was taken three times; the specific method of using the essence cream was to cleanse the face at 8 o'clock in the morning and apply 0.5±0.05g of each test sample essence cream on the face after cleansing; and to cleanse the face at 8 o'clock in the evening and apply 0.5±0.05g of each test sample essence cream on the face after cleansing. The experimental period lasted for 60 days; finally, the percentage of wrinkle depth reduction after 30 days and 60 days of treatment was calculated;

[0150] The specific test results are shown in Table 3 below:

[0151] Table 3

[0152]

[0153]

[0154] As shown in the test data in Table 3, the wrinkle depth reduction percentage of the essence cream provided by Application Examples 1-3 of the present invention after 60 days of use reached over 23%. This fully demonstrates that the various ingredients in the essence cream work together synergistically to effectively combat skin wrinkles, slow the aging process, reduce the formation of static lines, and improve dynamic lines by promoting the expression of skin elastin and cell proliferation. Comparative Application Examples 1-3 show that the absence of any of adenosine, sodium mannose phosphate, and mannose in the mitochondrial energy-enhancing composition reduces the wrinkle-reducing effect of the essence cream.

[0155] Test Example 4

[0156] Moisturizing performance test:

[0157] The Ningyan anti-wrinkle essence cream prepared in Application Examples 1-3 and 8-12 was tested to compare the changes in skin moisture content of the test subjects before and after use (the usage operation was kept consistent). A total of 80 volunteers were tested, including 56 females and 24 males, aged 20-40 years old with an average age of 28 years old. They were randomly divided into 8 groups, with 7 females and 3 males in each group.

[0158] The subjects washed their forearms and sat quietly for 30 minutes in a set humidity environment. A 3×3 cm 2 The left arm was used as the test area for the test sample, and the symmetrical area on the right arm was used as the blank control. The moisture content of each experimental part was detected by Corneometer CM825. The test was repeated 3 times and the average value was recorded.

[0159] The formula for calculating skin hydration rate is:

[0160] The average skin hydration rate of the subjects after using the samples for 2 hours is shown in Table 4:

[0161] Table 4

[0162] Group Skin hydration rate Application Example 1 44.5％ Application Example 2 43.7％ Application Example 3 42.9％ Application Example 8 35.6％ Application Example 9 32.4％ Application Example 10 36.6％ Application Example 11 38.3％ Application Example 12 36.5％

[0163] It can be seen from the data in Table 4 that the Ningyan anti-wrinkle essence cream involved in the present invention has a stronger moisturizing effect; the five ingredients in the moisturizer, namely butylene glycol, dipropylene glycol, hyaluronic acid, acetylated sodium hyaluronate and tremella polysaccharide, have a certain synergistic effect on the above-mentioned effects, and none of the five ingredients can be missing.

[0164] Test Example 5: Red blood cell hemolysis test

[0165] The red blood cell hemolysis test (RBC) is an alternative to the rabbit eye irritation test. Its basic principle is to evaluate the damage of chemicals to ocular tissue cells by measuring the amount of cellular hemoglobin dissolved and the degree of denaturation. The RBC test can be used to evaluate the eye irritation of cosmetics.

[0166] The red blood cell hemolysis test was conducted on the Ningyan anti-wrinkle essence cream prepared in Example 1-19. The test adopted the RBC test method and judgment criteria of the European Center for Validation of Alternative Methods. The specific judgment criteria are shown in Table 5, where HD 50 is the sample concentration when 50% of red blood cells are hemolyzed, DI is the protein denaturation index, and L / D is HD 50 Ratio to DI.

[0167] Table 5

[0168]

[0169]

[0170] The irritation results of the tested samples prepared in Application Examples 1-19 are shown in Table 6.

[0171] Table 6

[0172]

[0173]

[0174] From the sample irritation results in Table 6, it can be seen that the irritation classification of the Ningyan anti-wrinkle essence cream prepared in Application Examples 1-19 of the present invention is non-irritating, indicating that all components in the essence cream of the present invention are mild and non-irritating.

[0175] The applicant states that while the present invention uses the aforementioned embodiments to illustrate the composition for enhancing mitochondrial energy and its applications, the present invention is not limited to these embodiments, nor does it necessarily rely on these embodiments for implementation. Those skilled in the art will appreciate that any improvements to the present invention, equivalent substitutions for various raw materials in the present invention, addition of auxiliary ingredients, and selection of specific methods, etc., fall within the scope of protection and disclosure of the present invention.

[0176] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the scope of protection of the present invention.

[0177] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any appropriate manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A condensing anti-wrinkle cream, characterized in that: The Ningyan anti-wrinkle essence cream comprises, by weight, 1-15 parts of a composition for enhancing mitochondrial energy, 1-20 parts of a moisturizer, 0.1-1 parts of a thickener, 5-10 parts of a skin conditioner, 0.1-1 parts of an antioxidant, 1-10 parts of an emulsifier, 1-10 parts of a skin feel regulator, and 1-90 parts of deionized water; The composition for enhancing mitochondrial energy comprises adenosine, sodium mannose phosphate, mannose, creatine and yeast extract; The mass ratio of adenosine, sodium mannose phosphate, mannose, creatine and yeast extract is (3-5):(1-3):(1-2):(3-5):(0.1-1); The moisturizing agent is a combination of butylene glycol, dipropylene glycol, hyaluronic acid, acetylated sodium hyaluronate and tremella polysaccharide; The skin conditioning agent is a combination of niacinamide, lactobacillus / rice fermentation product, dipalmitoyl hydroxyproline, hydroxypropyl tetrahydropyrantriol, palmitoyl tripeptide-5, soluble collagen, and tetradecylaminobutyryl valinamidobutyric acid urea trifluoroacetate.

2. The anti-wrinkle cream according to claim 1, wherein: The thickener includes any one or a combination of at least two of acrylic acid (esters) / C10-30 alkyl acrylate cross-polymer, cetyl alcohol or stearyl alcohol.

3. The anti-wrinkle cream according to claim 1, wherein: The antioxidant includes any one of retinyl palmitate, p-hydroxyacetophenone or tocopheryl acetate, or a combination of at least two thereof.

4. The anti-wrinkle cream according to claim 1, wherein: The emulsifier includes any one or a combination of at least two of hydrogenated lecithin, phytosterols, C14-22 alcohol / C12-20 alkyl glucoside or ammonium acryloyldimethyltaurate / VP copolymer.

5. The anti-wrinkle cream according to claim 1, wherein: The skin feel regulator includes any one or a combination of at least two of sodium polyacryloyldimethyl taurate, polyisobutene, PEG-7 trimethylolpropyl coco ether, cetyl ethylhexanoate, diisostearyl malate, polydimethylsiloxane, rice germ oil, aluminum starch octenylsuccinate, squalane or jojoba seed oil.

6. The anti-wrinkle cream according to claim 1, wherein: The anti-wrinkle essence cream further comprises any one of a pH regulator, a preservative or a fragrance, or a combination of at least two of them.

7. The method for preparing the anti-wrinkle cream according to any one of claims 1 to 6, characterized in that: The preparation method comprises: (1) Add deionized water, moisturizer, and thickener to the water phase pot, stir and heat until warm; add emulsifier, skin feel regulator, and antioxidant to the oil phase pot, stir and heat until warm; (2) After the heat preservation is completed, the water and oil pots are pumped into the emulsifying pot, homogenized, stirred and cooled under vacuum, and the mitochondrial energy enhancing composition as claimed in claim 1 and the skin conditioner are added and mixed evenly to obtain the product.

8. The method for preparing the anti-wrinkle cream according to claim 7, wherein: The insulation temperature in step (1) is independently 80-85° C., and the insulation time is independently 10-20 minutes.

9. The method for preparing the anti-wrinkle cream according to claim 7, wherein: The homogenization temperature in step (2) is 78-85° C., the homogenization time is 5-10 min, the homogenization speed is 3000-5000 rpm, and the stirring speed is 20-30 rpm.

10. The method for preparing the anti-wrinkle cream according to claim 7, wherein: Step (2) is cooling to 30-40°C.

11. The method for preparing the anti-wrinkle cream according to claim 7, wherein: Step (2) further comprises adding any one or a combination of at least two of a pH regulator, a fragrance or a preservative.

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