Traditional Chinese medicine toothpaste with anti-inflammatory and hemostatic effects

Abstract

The application belongs to the technical field of traditional Chinese medicines, and specifically discloses a traditional Chinese medicine toothpaste with the functions of relieving inflammation and stopping bleeding. The traditional Chinese medicine toothpaste contains 0.1-1% of a traditional Chinese medicine polysaccharide composition and 65-85% of toothpaste raw materials and auxiliaries, and the rest is solvent; the traditional Chinese medicine polysaccharide composition contains ginger-prepared moslae polysaccharide; the traditional Chinese medicine polysaccharide composition can significantly reduce the bleeding time of mice and the swelling degree of the feet of rats, and achieve the functions of relieving inflammation and stopping bleeding. If the ginger-prepared moslae polysaccharide and the coralline polysaccharide are matched at a mass ratio of 1:0.5-1, the functions of relieving inflammation and stopping bleeding are better.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine toothpaste with the functions of relieving inflammation and stopping bleeding. BACKGROUND

[0002] At present, there are two kinds of toothpaste on the market, namely ordinary toothpaste and functional toothpaste, wherein the functional toothpaste is divided into chemical toothpaste and natural herbal toothpaste. According to relevant research, long-term use of chemical toothpaste can cause oral problems, such as long-term use of toothpaste with peroxide auxiliary whitening components can easily cause damage to tooth enamel and lead to tooth sensitivity. At present, the active components of Chinese herbal medicine added in Chinese herbal toothpaste on the domestic market mainly include flavonoids, alkaloids, saponins, volatile oils, coumarins, tannins, anthraquinones and organic acids, and there is less research on oral care products with polysaccharides as the main component. At present, there is no relevant literature report on the compatibility of ginger-prepared elsholtzia polycoside and polysaccharide of Sarcandra glabra for preparing traditional Chinese medicine toothpaste. SUMMARY

[0003] The technical problem to be solved by the present application is that, in view of the defects and deficiencies in the prior art that ginger-prepared elsholtzia polycoside and polysaccharide of Sarcandra glabra are not compatible for preparing traditional Chinese medicine toothpaste, a traditional Chinese medicine toothpaste with the functions of relieving inflammation and stopping bleeding is provided. The traditional Chinese medicine toothpaste contains 0.1-1% of a traditional Chinese medicine polysaccharide composition and 65-85% of toothpaste raw materials, and the balance is a solvent; the traditional Chinese medicine polysaccharide composition mainly contains ginger-prepared elsholtzia polycoside, and if the traditional Chinese medicine polysaccharide composition is compatible with ginger-prepared elsholtzia polycoside and polysaccharide of Sarcandra glabra at a mass ratio of 1:0.5-1.0, the prepared traditional Chinese medicine toothpaste has more significant functions of relieving inflammation and stopping bleeding.

[0004] The present application adopts the following technical scheme to achieve the purpose of the application.

[0005] A traditional Chinese medicine toothpaste with the functions of relieving inflammation and stopping bleeding contains 0.1-1% of a traditional Chinese medicine polysaccharide composition and 65-85% of toothpaste raw materials, and the balance is a solvent; the traditional Chinese medicine polysaccharide composition contains ginger-prepared elsholtzia polycoside; and the solvent is preferably pure water. It is proved by in-vitro cell tests that ginger-prepared elsholtzia polycoside can significantly reduce the expression of NO, IL-6 and IL-12 and increase the anti-inflammatory factor level of IL-4 and IL-10; and it is also proved by animal tests that ginger-prepared elsholtzia polycoside can significantly reduce the bleeding time of mice and the swelling degree of rat feet.

[0006] Polysaccharides are widely studied by scholars at home and abroad due to its green nature, small toxic and side effects, and less drug resistance. Jiangxi Xiangru is a native medicinal material in Jiangxi, which contains various nutrients and active ingredients such as volatile oil, flavonoids, organic acids, polysaccharides and mineral elements, and has many pharmacological effects such as antipyretic, sedative, analgesic, anti-inflammatory, antioxidant and immune enhancement. Among them, Jiangxi Xiangru polysaccharide is the main active ingredient in Jiangxi Xiangru. As a whole grass medicine, the processing of Jiangxi Xiangru is mainly cleaning and cutting, but the book Medical Primer first mentioned the processing of Jiangxi Xiangru with auxiliary materials - "stir-frying with ginger juice". Ginger, as a common medicinal processing auxiliary material, has the characteristics of relieving superficies and dispelling cold, warming middle and stopping vomiting, and can change the nature of different traditional Chinese medicines during processing to reduce toxicity and increase efficacy. The present application first proposes the processing of Jiangxi Xiangru polysaccharide with ginger and verifies its anti-inflammatory and hemostatic effects.

[0007] Further, the traditional Chinese medicine polysaccharide composition further comprises Sarcandra polysaccharide; the ratio of the ginger-processed Jiangxi Xiangru polysaccharide and the Sarcandra polysaccharide is 1:0.5-1 by mass fraction. The traditional Chinese medicine polysaccharide composition containing ginger-processed Jiangxi Xiangru polysaccharide and Sarcandra polysaccharide has stronger effect on reducing the bleeding time of mice and the foot swelling degree of rats, and more significant anti-inflammatory and hemostatic effect.

[0008] Sarcandra glabra can be used as medicine, which contains coumarins, flavonoids, phenolic acids, terpenes, polysaccharides and other active ingredients, and is often used for clearing heat and cooling blood, promoting blood circulation and removing spots, and dispelling wind and dredging collaterals. According to relevant reports, Sarcandra polysaccharide is a water-soluble component in Sarcandra glabra, and the Sarcandra glabra separation part Sarcandra polysaccharide is an effective part for anti-immune thrombocytopenic purpura, and some acidic polysaccharide structures in Sarcandra glabra also have good anti-inflammatory and antioxidant activities.

[0009] Further, the toothpaste raw material comprises but is not limited to abrasive, thickening agent, humectant, foaming agent.

[0010] Further, the toothpaste raw material further comprises one or more of preservative, flavoring agent, essence, colorant.

[0011] Preferably, the abrasive is preferably calcium carbonate and / or silicon dioxide; the thickening agent is preferably sodium carboxymethyl cellulose (CMC-Na) and / or xanthan gum; the humectant is preferably sorbitol and / or glycerol; the foaming agent is preferably sodium dodecyl sulfate (SDS); the preservative is preferably nipagin; the flavoring agent is preferably sodium saccharin; and the essence is preferably plant essential oil, such as peppermint oil, spearmint essential oil, etc.

[0012] The ginger preparation of the invention, the extraction method of elsholtzia polystachya polysaccharide, comprising the following steps: S1 preparation of ginger juice; S2 preparation of elsholtzia polystachya with ginger juice; S3 extraction of ginger preparation of elsholtzia polystachya polysaccharide: after the preparation of elsholtzia polystachya with ginger juice, the pretreatment, hot water extraction, vacuum concentration, Sevag method to remove free protein, ethanol precipitation, drying and grinding steps, get ginger preparation of elsholtzia polystachya polysaccharide.

[0013] Further, the preparation of ginger juice in step S1 is: take fresh ginger, wash the mud and sand outside the ginger with clean water, wash and cut into slices, dry, after the surface of ginger slices is dry, add distilled water and heat decoct 2-4 times, combine the filtrate and concentrate under reduced pressure, concentrate to the ginger extract containing 0.8-1.2g / mL of crude drug, get ginger juice.

[0014] Preferably, the mass volume ratio of ginger to distilled water is 1:3-5.

[0015] Further, the preparation of elsholtzia polystachya with ginger juice in step S2 is: take elsholtzia polystachya decoction pieces, put them into airtight container, put in equal volume of ginger juice, put in cool place, after moistening, fry, get elsholtzia polystachya prepared with ginger juice.

[0016] Preferably, the moistening time is 4-8h, and the frying time is 6-10min.

[0017] Further, the pretreatment in step S3 is: take elsholtzia polystachya prepared with ginger juice, add distilled water, and pretreat according to ultrasonic extraction conditions.

[0018] Preferably, the mass volume ratio of ginger preparation of elsholtzia polystachya to distilled water is 1:15-25, the ultrasonic time is 8-12min, and the ultrasonic power is 80w.

[0019] Further, the hot water extraction and vacuum concentration in step S3 are: after the pretreatment of ginger preparation of elsholtzia polystachya, hot water extraction, and vacuum concentration of the obtained filtrate to stop when the ginger preparation of elsholtzia polystachya contains 0.15-0.25g / mL of crude drug.

[0020] Preferably, the hot water extraction temperature is 85-95℃, the hot water extraction time is 1-2h; the vacuum concentration temperature is 52-60℃, and the vacuum concentration rotation speed is 40-60rpm / min.

[0021] Further, the Sevag method for removing free proteins in step S3 is as follows: first, Sevag reagent is added to the concentrated solution in a volume ratio of 4-6, and then the solution is shaken and centrifuged, and the supernatant is collected after the solution is layered and centrifuged; second, Sevag reagent is added to the supernatant in a volume ratio of 4-6, and then the solution is shaken and centrifuged to obtain the purified supernatant; and the supernatant is treated repeatedly 0-2 times according to the above conditions.

[0022] Preferably, the Sevag reagent is prepared from chloroform and n-butanol in a ratio of 4:1; and the centrifugal speed is 2500-3500 rpm / min, and the centrifugal time is 4-8 min.

[0023] Further, the ethanol precipitation in step S3 is as follows: 92%-99% ethanol is added to the supernatant, and the solution is placed in a refrigerator at 4°C for alcohol precipitation overnight.

[0024] Preferably, the volume ratio of the supernatant to 92%-99% ethanol is 1:2.5-3.5.

[0025] Further, the drying and grinding in step S3 is as follows: after alcohol precipitation overnight, the supernatant is removed, the bottom precipitate is collected by centrifugation, and then the precipitate is dried and ground into powder to obtain the ginger-prepared mosla herb polysaccharide.

[0026] Preferably, the centrifugation is performed at 4500-5000 rpm / min for 8-12 min to collect the bottom precipitate, and the drying is freeze-drying.

[0027] The extraction method of the polysaccharide of Sarcandra glabra according to the present application comprises the following steps:

[0028] S10: taking Sarcandra glabra whole herb decoction pieces, crushing them appropriately, adding distilled water, refluxing and extracting multiple times, and then filtering and collecting the extract to obtain a concentrated solution;

[0029] S20: adding ethanol to the concentrated solution, and placing the solution in a refrigerator at 4°C for alcohol precipitation overnight;

[0030] S30: discarding the supernatant, centrifuging the lower layer precipitate to collect the bottom precipitate, freeze-drying the precipitate, and then grinding the dried precipitate into powder to obtain the polysaccharide of Sarcandra glabra.

[0031] Further, in step S10, the solid-liquid ratio of the Sarcandra glabra whole herb decoction pieces to distilled water is 1:15-25, the reflux extraction is performed 2-4 times, and each time is 3-5 h; the concentration temperature is 52-60°C, the concentration speed is 40-60 rpm / min, and the concentration is stopped when the concentration of Sarcandra glabra crude drug in the concentrated solution is 0.08-0.12 g / mL.

[0032] Further, the concentrated liquid in step S20 is added with ethanol in a volume ratio of 1 volume of the concentrated liquid to 2-4 volumes of 92%-99% ethanol.

[0033] Further, the centrifugation in step S30 is carried out at 2500-3500 rpm / min for 8-12 min.

[0034] The traditional Chinese medicine toothpaste obtained by the present application has significant anti-inflammatory and hemostatic effects. The present application experimentally explores and researches and develops the anti-inflammatory and hemostatic effects of polysaccharides before and after processing and the compatibility of Sarcandra glabra polysaccharides, thereby providing a theoretical basis for the development and utilization of traditional Chinese medicine toothpaste.

[0035] Beneficial effects:

[0036] (1) The present application uses ginger-processed Elsholtzia ciliata polysaccharide as the main component, and the traditional Chinese medicine toothpaste obtained thereby has significant anti-inflammatory and hemostatic effects; if appropriately combined with Sarcandra glabra polysaccharide, the anti-inflammatory and hemostatic effects are better. Through animal experiments, the anti-inflammatory and hemostatic effects are ranked as follows: Example 5 (containing ginger-processed Elsholtzia ciliata polysaccharide and Sarcandra glabra polysaccharide) > Example 4 (containing ginger-processed Elsholtzia ciliata polysaccharide) > Comparative Example 1 (containing Elsholtzia ciliata composition) > Comparative Example 2 (Yunnan Baiyao toothpaste) ≈ Comparative Example 3 (Sarcandra glabra toothpaste) > Comparative Example 4 (Yunnan Tianqi traditional Chinese medicine toothpaste). Example 4 in the specification of the present application also proves that: the traditional Chinese medicine toothpaste prepared in Example 5 (containing 0.15% ginger-processed Elsholtzia ciliata polysaccharide and 0.1% Sarcandra glabra polysaccharide) can significantly increase the platelet aggregation rate and the content of platelet secreted TXB2 in fresh blood of rabbits under the same dosage concentration of toothpaste samples compared with Comparative Examples 1-4, and is also higher than that of the traditional Chinese medicine toothpaste prepared in Example 4 (containing 0.21% ginger-processed Elsholtzia ciliata polysaccharide), which indicates that ginger-processed Elsholtzia ciliata polysaccharide combined with appropriate Sarcandra glabra polysaccharide has a synergistic effect, and the anti-inflammatory and hemostatic effects are better; through a rat gingivitis model experiment, it is also proved that the traditional Chinese medicine toothpaste prepared in Example 5 of the present application can significantly reduce the levels of GI (gingival index), PI (plaque index) and BOP (bleeding index) compared with Comparative Examples 1-4 under the same conditions, and has a better effect on improving anti-inflammatory factors IL-4 and IL-10 and reducing inflammatory factors IL-1β, TNF-α and IL-6, and the effect is also better than that of the traditional Chinese medicine toothpaste prepared in Example 4, which indicates that ginger-processed Elsholtzia ciliata polysaccharide combined with appropriate Sarcandra glabra polysaccharide has a synergistic effect, and the anti-inflammatory and hemostatic effects are better.

[0037] (2), The application proves that the bleeding time of mice has no significant difference by the mouse tail bleeding test, and the bleeding time of mice can be significantly reduced by the elsholtziae polysaccharide and the polysaccharide of corallium rubrum, and the bleeding time of mice in the middle dose group is significantly reduced, and the treatment effect is close to that of Yunnan white medicine, which shows that the polysaccharide of corallium rubrum has good hemostatic effect. When the polysaccharide of corallium rubrum and the elsholtziae polysaccharide, or the polysaccharide of corallium rubrum and the elsholtziae polysaccharide are matched in a mass ratio of 0.7:1, compared with the blank control group, the polysaccharide of corallium rubrum+elsholtziae polysaccharide group can significantly reduce the bleeding time of mice, which shows that the elsholtziae polysaccharide matched with the appropriate polysaccharide of corallium rubrum has a synergistic effect, and the anti-inflammatory and hemostatic effect is better.

[0038] (3), The application proves that the elsholtziae polysaccharide, the elsholtziae polysaccharide and the polysaccharide of corallium rubrum can significantly reduce the rat foot swelling, and the elsholtziae polysaccharide in the middle dose group has significant difference, which shows that the elsholtziae polysaccharide can reduce the rat foot swelling.

[0039] (4), The application proves that the elsholtziae polysaccharide in the high dose group can significantly reduce the expression of pro-inflammatory factors IL-6, IL-12 and NO, and increase the expression of anti-inflammatory factors IL-4 and IL-10; and the elsholtziae polysaccharide in the middle dose group can significantly reduce the expression of IL-6, IL-12 and NO, and increase the expression of IL-4 and IL-10 in the high dose group, which shows that the elsholtziae polysaccharide processed by ginger juice has stronger anti-inflammatory effect.

[0040] (5), The preparation method of the elsholtziae polysaccharide and the polysaccharide of corallium rubrum has the advantages of unique method, simple process, convenient operation, low cost, high polysaccharide yield and the like. BRIEF DESCRIPTION OF DRAWINGS

[0041] Figure 1 : Determination of mouse bleeding time;

[0042] Figure 2 : Determination of rat foot swelling;

[0043] Figure 3 : Effect of elsholtziae polysaccharide on RAW264.7 cell activity;

[0044] Figure 4 : Effect of elsholtziae polysaccharide on RAW264.7 cell activity;

[0045] Figure 5 : Effect of different concentrations of LPS on RAW264.7 cell activity;

[0046] Figure 6Effect of different concentrations of LPS on NO release of RAW264.7 cells

[0047] Figure 7 Effect of polysaccharide on expression of proinflammatory factor NO of RAW264.7 cells induced by LPS

[0048] Figure 8 Effect of polysaccharide on expression of proinflammatory factor IL-6 of RAW264.7 cells induced by LPS

[0049] Figure 9 Effect of polysaccharide on expression of proinflammatory factor IL-12 of RAW264.7 cells induced by LPS

[0050] Figure 10 Effect of polysaccharide on expression of anti-inflammatory factors IL-4 and IL-10 of RAW264.7 cells induced by LPS

[0051] Figure 11 Effect of different concentrations of each toothpaste sample on platelet aggregation rate in rabbit platelet biological model

[0052] Figure 12 Effect of different concentrations of each toothpaste sample on content of TXB2 secreted by platelets in rabbit platelet biological model

[0053] Figure 13 Analysis of proliferation and toxicity of HGECs cells treated by each toothpaste sample

[0054] Figure 14 Determination of IL-4 and IL-10 secreted by HGECs cells treated by each toothpaste sample and induced by LPS

[0055] Figure 15 Determination of NO and IL-1β secreted by HGECs cells treated by each toothpaste sample and induced by LPS

[0056] Figure 16 Determination of IL-6 and TNF-α secreted by HGECs cells treated by each toothpaste sample and induced by LPS

[0057] Figure 17 Effect of each toothpaste sample on GI (gingival index), PI (plaque index) and BOP (bleeding index) of rats

[0058] Figure 18 Effect of each toothpaste sample on content of anti-inflammatory factors (IL-4 and IL-10)

[0059] Figure 19 Effect of each toothpaste sample on content of proinflammatory factor IL-1β

[0060] Figure 20 : Effect of each toothpaste sample on the content of inflammatory factor IL-6;

[0061] Figure 21 : Effect of each toothpaste sample on the content of inflammatory factor TNF-α. DETAILED DESCRIPTION

[0062] Example 1: Preparation of Elsholtzia ciliate polysaccharide

[0063] Take 40 g of Elsholtzia ciliate decoction pieces, first ultrasonic-assisted extraction, according to the solid-liquid ratio 1:20, add 800 mL of distilled water, ultrasonic power 80 w, ultrasonic time 10 min, then hot water extraction, extraction temperature 90 ℃, extraction time 1 h, filter to get the filtrate, using vacuum concentration method, set the concentration temperature 55 ℃, concentrated to 1 / 4 of the total volume to stop, at this time the concentrated liquid contains Elsholtzia ciliate crude drug mass concentration of 0.22 g / mL, concentrated liquid 180 mL, add Sevag reagent 36 mL with volume of 1 / 5 of the concentrated liquid, shake, stand for layering and then centrifuge, centrifugal speed 3000 rpm / min, 5 min, take the supernatant, repeat three times; add 3 times volume of 95% ethanol 450 mL to the supernatant 150 mL or so, put it in the 4 ℃ refrigerator to precipitate overnight; discard the supernatant, the lower precipitate is centrifuged at 4800 rpm / min for 10 min to collect the bottom precipitate, put the precipitate into the freeze dryer to dry, grind into powder after drying, get Elsholtzia ciliate polysaccharide 1.38 g. The yield is 3.45%.

[0064] Example 2: Preparation of Elsholtzia ciliate polysaccharide prepared by ginger

[0065] The fresh ginger is washed with clean water to remove the dirt on the surface, and then sliced. After the surface of the ginger slices is dried, distilled water is added at a material-to-liquid ratio of 1:4. The mixture is heated and boiled for 3 times, each for 30 min. The filtrates are combined and concentrated under reduced pressure until the ginger extract contains 1 g / mL of ginger crude drug. The prepared Jiangxiangruo is obtained by adding 40 g of Jiangxiangruo decoction pieces into a No. 6 self-sealing bag, adding 40 mL of the ginger extract, and then placing the mixture in a cool place for 6 hours of soaking and 8 minutes of frying. The Jiangxiangruo prepared by the ginger extract is obtained by ultrasonic-assisted extraction. Distilled water 800 mL is added at a material-to-liquid ratio of 1:20. The ultrasonic power is 80 W, and the ultrasonic time is 10 min. The ginger-prepared Jiangxiangruo is pretreated. The ginger-prepared Jiangxiangruo treated by ultrasonic is extracted by hot water immersion method at an extraction temperature of 90℃ and an extraction time of 1 h. The obtained extract is filtered and concentrated. The concentration temperature is set to 55℃, and the concentration speed is set to 50 rpm / min. The concentration is stopped when the total volume is 1 / 4. At this time, the concentration liquid contains 0.22 g / mL of Jiangxiangruo crude drug. 180 mL of the concentrated liquid is added to 36 mL of Sevag reagent with a volume of 1 / 5 of the concentrated liquid. After shaking and standing for stratification, centrifugation is performed at a speed of 3000 rpm / min for 5 min. The supernatant is collected, and the operation is repeated three times. About 150 mL of the supernatant is added to 3 times the volume of 95% ethanol 450 mL, and then placed in a 4℃ refrigerator for alcohol precipitation overnight. The supernatant is discarded, and the lower precipitate is collected by centrifugation at 4800 rpm / min for 10 min. The precipitate is dried in a freeze dryer, ground into powder after drying, and 1.514 g of ginger-prepared Jiangxiangruo polysaccharide is obtained, with an extraction rate of 3.78%.

[0066] Example 3: Preparation of Sarcandra polysaccharide

[0067] 40 g of Sarcandra whole herb decoction pieces are taken and appropriately crushed. Distilled water 800 mL is added at a material-to-liquid ratio of 1:20. The mixture is refluxed and extracted for 3 times, each for 4 h. The combined extract is filtered and concentrated under reduced pressure. The concentration temperature is set to 55℃, and the concentration speed is set to 50 rpm / min. The concentration is stopped when the total volume is 1 / 4 to 1 / 5. 500 mL of the concentrated liquid is obtained. At this time, the concentrated liquid contains 0.08 g / mL of Sarcandra crude drug. 500 mL of the concentrated liquid is added to 3 times the volume of 95% ethanol 1500 mL, and then placed in a 4℃ refrigerator for alcohol precipitation overnight. The supernatant is discarded, and the lower precipitate is collected by centrifugation at 3000 rpm / min for 10 min. The precipitate is dried in a freeze dryer, ground into powder after drying, and 2.523 g of Sarcandra polysaccharide is obtained, with a yield of 6.31%.

[0068] Example 4: Preparation of toothpaste small sample containing ginger-prepared Jiangxiangruo polysaccharide

[0069] Ratio: in mass percentage: anti-inflammatory and hemostatic agent ginger prepared mosla herb polysaccharide 0.21% (equivalent to crude drug amount 0.21% / 3.78%=0.05g crude drug / g), foaming agent SDS (sodium dodecyl sulfate) 1%, abrasive agent calcium carbonate 40% and silicon dioxide 3%, thickening agent CMC-Na (sodium carboxymethyl cellulose) 0.8% and xanthan gum 0.4%, flavoring agent sodium saccharin 0.1%, humectant glycerol 3% and sorbitol 21%, preservative nipagin 0.2%, essence 1%, pure water 29.3%.

[0070] Preparation method: according to the above formula ratio, first, SDS, silicon dioxide, calcium carbonate, CMC-Na and other raw materials are weighed and ground; then, sodium saccharin, glycerol, sorbitol, xanthan gum, nipagin, essence and other raw materials are dissolved in a certain amount of deionized water, and after stirring for 50 minutes, no particles are basically present, ginger prepared mosla herb polysaccharide is added according to mass, and stirring is continued for 30 minutes to complete the process of paste preparation, and after vacuum defoaming and molding, the tube is filled.

[0071] Example 5: Preparation of toothpaste sample containing ginger prepared mosla herb polysaccharide and spongilla polysaccharide

[0072] Preparation method: according to the above formula ratio, first, SDS, silicon dioxide, calcium carbonate, CMC-Na and other raw materials are weighed and ground; then, sodium saccharin, glycerol, sorbitol, xanthan gum, nipagin, essence and other raw materials are dissolved in a certain amount of deionized water, and after stirring for 50 minutes, no particles are basically present, ginger prepared mosla herb polysaccharide and spongilla polysaccharide are added according to mass, and stirring is continued for 30 minutes to complete the process of paste preparation, and after vacuum defoaming and molding, the tube is filled.

[0073] Comparative Example 1: Preparation of mosla herb toothpaste

[0074] Preparation of Elsholtzia stauntonii composition: 500g of Elsholtzia stauntonii, 400g of Astragalus membranaceus, 300g of Chrysanthemum morifolium, and 150g of Lonicera japonica are first ground into powder. Then the ground materials are mixed and soaked in water for 1 hour. The soaked materials are decocted in water for 3 times, with 1 hour, 0.5 hour, and 0.5 hour for each time. The decoction filtrate is obtained by filtration. The decoction filtrate is then combined and concentrated under vacuum at <-0.08 MPa until the concentration of the crude drug in the filtrate reaches 10g / mL, and the concentrated filtrate is used as the additive of the Chinese herbal toothpaste.

[0075] Toothpaste ratio: by mass percentage, 0.5% of Elsholtzia stauntonii composition (0.1 mL / g of composition concentration, 0.5% equivalent to 0.05g of crude drug per gram), 1% of foaming agent SDS (sodium dodecyl sulfate), 40% of abrasive calcium carbonate and 3% of silicon dioxide, 0.8% of thickening agent CMC-Na (sodium carboxymethyl cellulose) and 0.4% of xanthan gum, 0.1% of flavoring agent sodium saccharin, 3% of humectant glycerol and 21% of sorbitol, 0.2% of preservative nipagin, 1% of essence, and 29% of pure water.

[0076] Preparation method: according to the above formula ratio, first grind the raw materials such as SDS, silicon dioxide, calcium carbonate, and CMC-Na; then dissolve the raw materials such as sodium saccharin, glycerol, sorbitol, xanthan gum, nipagin, and essence in a certain amount of deionized water, stir for 50 minutes, and then add the Elsholtzia stauntonii composition, continue to stir for 30 minutes to complete the toothpaste preparation process, and then shape by vacuum defoaming and fill the tube.

[0077] Comparative Example 2: Yunnan Baiyao toothpaste

[0078] Commercially available. Yunnan Baiyao Group Co., Ltd., 185g, 20201222.

[0079] Comparative Example 3: grass coral toothpaste

[0080] Commercially available. Nanchang Chengzhi Co., Ltd., 228g, 20201125.

[0081] Comparative Example 4: Yunnan Tianqi Chinese medicine toothpaste

[0082] Commercially available. Foshan Huangqi Jiachun Biological Engineering Co., Ltd., 180g, 20201015.

[0083] Experimental Example 1: determination of hemostatic time in mice

[0084] One hundred and thirty healthy male Kunming mice, weighing 20±20g, were raised in a SPF level barrier system with an environmental temperature of 20-23℃ and humidity of 45%-55%. The mice were randomly divided into 13 groups (see Table 1). Figure 1The mice were divided into 10 groups (10 mice in each group) as shown in the table, namely, normal control group (NS group), positive group (Yunnan white medicine group 50 mg / kg, YNBY group), low, medium and high dose groups of Elsholtzia kachiniana polysaccharide (35, 70, 140 mg / kg, namely JXR-L, JXR-M, JXR-H three groups), low, medium and high dose groups of ginger processed Elsholtzia kachiniana polysaccharide (35, 70, 140 mg / kg, namely JZJXR-L, JZJXR-M, JZJXR-H three groups), low, medium and high dose groups of Sarcandra glabra (35, 70, 140 mg / kg, namely CSH-L, CSH-M, CSH-H three groups), Sarcandra glabra polysaccharide + Elsholtzia kachiniana polysaccharide compatibility group (25 mg / kg + 35 mg / kg, CSH+JXR group) and Sarcandra glabra polysaccharide + ginger processed Elsholtzia kachiniana polysaccharide compatibility group (25 mg / kg + 35 mg / kg, CSH+JZJXR group). The normal control group was given the same volume of normal saline by gavage, and the administration groups were given different concentrations of polysaccharide solution by gavage once a day for 6 consecutive days. After 30 min of the 6th administration, the mice were cut at a distance of 0.3 cm from the tail tip, and the bleeding time was recorded from the start of blood overflow. The bleeding time of each group of mice was determined Figure 1 In the figure: * represents a significant difference (P<0.05) compared with the normal control (NS) group, ** represents a significant difference (P<0.01) compared with the normal control (NS) group, and the same below.

[0085] To Figure 1 Analysis shows that after gavage administration of different concentrations of Elsholtzia kachiniana polysaccharide and ginger processed Elsholtzia kachiniana polysaccharide, the bleeding time of mice decreases to different degrees with the increase of the concentration of the administered drug. Among them, compared with the normal control group, the high dose group of Elsholtzia kachiniana polysaccharide and the medium and high dose groups of ginger processed Elsholtzia kachiniana polysaccharide have significant differences (P<0.05 or P<0.01), which indicates that the hemostatic effect of ginger processed Elsholtzia kachiniana polysaccharide is better than that of Elsholtzia kachiniana polysaccharide.

[0086] When different concentrations of Sarcandra glabra polysaccharide were administered by gavage, the bleeding time of mice was significantly reduced. Among them, compared with the normal control group, the medium dose group of Sarcandra glabra polysaccharide had a significant difference (P<0.05), and the bleeding time was significantly reduced in the high dose group (P<0.01). Compared with the positive group, although the high dose group of Sarcandra glabra had no statistical significance, the bleeding time of the mice was lower than that of the positive group. Compared with the normal control group, the polysaccharide in the compatibility group had no statistical significance in Sarcandra glabra + Elsholtzia kachiniana polysaccharide, but had a significant difference (P<0.01) in Sarcandra glabra + ginger processed Elsholtzia kachiniana polysaccharide. This indicates that the compatibility of low-dose Sarcandra glabra polysaccharide and ginger processed Elsholtzia kachiniana polysaccharide has a significant hemostatic effect.

[0087] Experimental Example 2: Determination of rat paw swelling

[0088] Healthy male SD rats 112, weighing 200 ± 20 g, were raised in SPF barrier system, the ambient temperature was 20-23℃, humidity 45%-55%. The rats were randomly divided into 14 groups (grouping see Figure 2 The rats were randomly divided into 14 groups (grouping see Figure 2 The rats were randomly divided into 14 groups (grouping see

[0089] The rats were randomly divided into 14 groups (grouping see Figure 2 Analysis can be seen: after the rats were injected with carrageenan, the toe volume was measured at 0, 1, 2, and 4h. Among them, at 0, 1 and 2h, the toe volume of rats in the drug group increased to varying degrees, but at 4h, compared with the model group, the positive group had significant difference (P<0.01); Jiangxiangru polysaccharide and Sarcandra polysaccharide had statistical significance in the high dose group (P<0.05), while Jiangxianru polysaccharide had significant difference in the middle dose group (P<0.05), which indicated that Jiangxianru polysaccharide could reduce the swelling of rat feet.

[0090] When low-dose Sarcandra polysaccharide was used in combination with low-dose Jiangxianru polysaccharide and Jiangxianru polysaccharide, compared with the model group, CSH+JZJXR polysaccharide had significant difference (P<0.05) at 4h after injection of carrageenan, while CSH+JXR polysaccharide had no such difference. This shows that low-dose Sarcandra polysaccharide and Jiangxianru polysaccharide can reduce the swelling of rat feet.

[0091] Example 3

[0092] (1) Effect of Jiangxianru polysaccharide and Jiangxianru polysaccharide on the activity of RAW264.7 cells:

[0093] RAW264.7 cells were cultured in high-sugar medium containing 10% fetal bovine serum, and when the cells grew to the logarithmic phase, the cell concentration was adjusted to 2*10 5 After 24 hours of culture in a 37-degree, 5% CO2 incubator, the medium was replaced with different concentrations of LPS, with a final concentration of 0.1, 0.2, 0.5, 1, 2 μg / mL, and six replicate wells were set up at each concentration as the drug group. At the same time, the medium without drugs was set up as the control group, and the medium without cells was set up as the blank group. After 24 hours of culture, the culture solution was discarded, 10% MTT-containing medium was added to each well, and the culture was continued for 4 hours. Then the culture was terminated, the medium in the wells was carefully aspirated, 150 μl of DMSO was added to each well, and the mixture was gently shaken on a shaking bed for 10 minutes. The absorbance at 490 nm of each well was detected on an enzyme marker instrument. Figure 3 The effect of elsholtzia polycarpos polysaccharide on RAW264.7 cell activity; Figure 4 The effect of elsholtzia polycarpos polysaccharide on RAW264.7 cell activity;

[0094] The effect of elsholtzia polycarpos polysaccharide on RAW264.7 cell activity; Figure 3 The effect of elsholtzia polycarpos polysaccharide on RAW264.7 cell activity; Figure 4 Analysis shows that different concentrations of elsholtzia polycarpos polysaccharide and ginger elsholtzia polycarpos polysaccharide were used to intervene in RAW264.7 cells to screen the intervention concentration, and MTT method was used to detect the effect of different concentrations of elsholtzia polycarpos polysaccharide and ginger elsholtzia polycarpos polysaccharide on cell proliferation rate. The results show that compared with the control group, when the two polysaccharides are below 10 μg / mL, there is no toxic effect on the cells, and further increasing the concentration of polysaccharide can significantly inhibit cell proliferation (P<0.05), and it is dose-dependent.

[0095] (2) MTT detection of the effect of LPS (lipopolysaccharide) on RAW264.7 cell activity:

[0096] The cell concentration was adjusted to 2*10 5 After 24 hours of culture in a 37-degree, 5% CO2 incubator, the medium was replaced with different concentrations of LPS, with a final concentration of 0.1, 0.2, 0.5, 1, 2 μg / mL, and six replicate wells were set up at each concentration as the drug group. At the same time, the medium without drugs was set up as the control group, and the medium without cells was set up as the blank group. After 24 hours of culture, the culture solution was discarded, 10% MTT-containing medium was added to each well, and the culture was continued for 4 hours. Then the culture was terminated, the medium in the wells was carefully aspirated, 150 μl of DMSO was added to each well, and the mixture was gently shaken on a shaking bed for 10 minutes. The absorbance at 490 nm of each well was detected on an enzyme marker instrument. Figure 5The effect of different concentrations of LPS on the activity of RAW264.7 cells.

[0097] The effect of different concentrations of LPS on the activity of RAW264.7 cells. Figure 5 Analysis shows that after treating cells with different concentrations of lipopolysaccharide, the MTT method is used to detect the cell activity of LPS on RAW264.7 cells. Compared with the normal control group, there is a significant difference (P<0.05) when the concentration is 2 μg / mL, which has an effect on the activity of the cells. The results show that when the concentration of LPS is 0-1 μg / mL, it has no effect on the activity of the cells, which can provide a reference for the selection of LPS concentration.

[0098] (3) The effect of LPS (lipopolysaccharide) on the NO content of RAW264.7 cells:

[0099] According to the above method, the cells are cultured, and after 24 hours of drug incubation, the cell supernatant is collected and the NO content is measured according to the NO kit operation. The standard is diluted into concentrations of 0, 1, 2, 5, 10, 20, 40, 60, and 100 μM, respectively, to make a standard curve. 50 μl of sample is added to each well, followed by the addition of Gr iess1 and Gr iess2, respectively. The absorbance at 450 nm is detected on the enzyme marker and the NO content is calculated. Figure 6 The effect of different concentrations of LPS on the NO release of RAW264.7 cells.

[0100] The effect of different concentrations of LPS on the NO release of RAW264.7 cells. Figure 6 Analysis shows that the NO kit is used to detect the effect of different concentrations of LPS on the NO release of RAW264.7 macrophages. With the increase of LPS concentration, the content of NO is positively correlated with the concentration of LPS. Compared with the normal group, LPS with a concentration of 0.1-2 μg / mL has a significant difference (P<0.01). This indicates that LPS can induce inflammation when the concentration is 0.1-2 μg / mL. Combined with the cell activity test, the concentration of LPS is selected as 1 μg / mL, which can be used for subsequent cell inflammation modeling.

[0101] (4) Detection of LPS-induced RAW264.7 cell inflammatory factors by elsholtzia polycarpos polysaccharide and ginger elsholtzia polycarpos polysaccharide:

[0102] According to the above method, the cells are cultured and inoculated in a 96-well plate. After 24 hours of incubation, the culture medium is carefully aspirated and washed twice with PBS. Different concentrations of polysaccharide solution (2 μg / mL, 5 μg / mL, 10 μg / mL) are added, and 1 μg / mL of LPS is added after 1 hour. At the same time, LPS groups and normal control groups are set up, with 3 replicate wells in each group. After 24 hours of incubation, the cell supernatant is collected and the inflammatory factors NO, IL-6, IL-12, IL-4, and IL-10 are detected by ELISA kit. Figure 7Effect of polysaccharide on expression of proinflammatory factor NO in LPS-induced RAW264.7 cells; Figure 8 Effect of polysaccharide on expression of proinflammatory factor IL-6 in LPS-induced RAW264.7 cells; Figure 9 Effect of polysaccharide on expression of proinflammatory factor IL-12 in LPS-induced RAW264.7 cells; Figure 10 Effect of polysaccharide on expression of anti-inflammatory factors IL-4 and IL-10 in LPS-induced RAW264.7 cells.

[0103] Effect of polysaccharide on expression of proinflammatory factor NO in LPS-induced RAW264.7 cells; Figure 7 , Figure 8 , Figure 9 , Figure 10 Analysis shows that compared with the blank control group, the contents of NO, IL-6 and IL-12 in the model control group are significantly increased, and the contents of IL-4 and IL-10 are significantly decreased (P<0.05 or P<0.01). Compared with the model control group, the contents of NO, IL-6 and IL-12 in the Jiangxiangru polysaccharide and ginger processed Jiangxiangru polysaccharide groups at medium and high doses are reduced to different degrees, and the contents of IL-4 and IL-10 are significantly increased (P<0.05 or P<0.01). Among them, although there is no significant difference between the ginger processed Jiangxiangru polysaccharide group and the Jiangxiangru polysaccharide group, the contents of proinflammatory factors NO, IL-6 and IL-12 in the ginger processed Jiangxiangru polysaccharide group are lower than those in the Jiangxiangru polysaccharide group at high dose, and the anti-inflammatory factors are opposite, which indicates that the anti-inflammatory effect of Jiangxiangru polysaccharide processed by ginger is better.

[0104] Example 4: Comparison of anti-inflammatory and hemostatic effects of six toothpastes

[0105] The toothpaste samples of Example 4 (containing ginger processed Jiangxiangru polysaccharide), Example 5 (containing ginger processed Jiangxiangru polysaccharide and Sanguo polysaccharide), Comparative Example 1 (containing Jiangxiangru composition), Comparative Example 2 (Yunnan Baiyao toothpaste), Comparative Example 3 (Sanguo toothpaste), and Comparative Example 4 (Yunnan Tianqi toothpaste) were extracted in a water bath, the filtrate was centrifuged to obtain the supernatant, and then rotary evaporation (40℃, 75rpm / min) was performed under reduced pressure to the original volume of the toothpaste. The samples were dried in a vacuum drying oven for 6h, and then freeze-dried to obtain the test samples of the toothpastes. The samples were reconstituted with 0.9% physiological saline or DMEM medium to 0.8%, 0.4%, 0.2%, 0.1%, 0.05%, and 0.22μm, respectively, and then filtered with a 0.22μm filter membrane. The samples were stored at 4℃ for standby use. In vivo animal experiments and in vitro experiments were carried out under the same conditions, and the anti-inflammatory and hemostatic effects were compared.

[0106] Design of experimental scheme and analysis of results:

[0107] (1) The effects of toothpaste on gingival hemostasis were evaluated by studying the platelet aggregation rate and the content of thromboxane B2 (TXB2):

[0108] Fresh rabbit blood, after centrifugation, the platelet density is adjusted to 1x10 12 / L, first detect the absorbance OD0 of the blank, add different toothpaste samples (5uL / well), and shake slightly. Then, place the 96-well plate in a constant temperature incubator at 37°C for 5 minutes to fully react. Subsequently, add 5uL of adenosine diphosphate ADP solution and shake slightly to induce platelet aggregation. After the platelets appear to aggregate, the turbidity in the system will change. The change in the OD value detected by the enzyme marker method is used to calculate the platelet aggregation rate.

[0109] The platelet aggregation rate is the earliest response of hemostatic function, and when the platelet aggregation rate increases, the blood coagulation state is presented, Figure 11 The influence of the concentration of each toothpaste sample on the platelet aggregation rate in the rabbit platelet biological model is shown in Table 1. Figure 11 As the concentration of toothpaste increases, the platelet aggregation rate increases, and Example 5 has a significant difference (P<0.05, *) compared with other groups at each concentration.

[0110] The content of TXB2 secreted by platelets can reflect the activity of platelets, and the higher the content of TXB2, the more active the physiological activity of platelets, Figure 12 The influence of the concentration of each toothpaste sample on the content of TXB2 secreted by platelets in the rabbit platelet biological model is shown in Table 2. Figure 12 It can be seen that Example 5 has a significant difference (P<0.05, *) compared with other groups at a concentration of 0.1%-0.4%, and Example 5 and Example 4 have a significant difference (P<0.05, *) compared with other groups at a concentration of 0.8%.

[0111] (2) CCK-8 method is used to detect the proliferation and toxicity of toothpaste samples on HGECs, and the optimal toothpaste sample concentration value for HGECs is selected according to the cell survival rate after drug addition:

[0112] Figure 13 The proliferation and toxicity of each toothpaste sample on HGECs are analyzed, and the optimal toothpaste sample concentration value for HGECs is selected. Figure 13 It can be seen that after adding toothpaste samples with concentration gradients (containing 10ug / mL LPS) and fully incubating, CCK-8 reagent is added, and the absorbance of each well at 550nm is measured by an enzyme marker. The toothpaste sample concentration of 80%-100% cell survival rate is the safest and most suitable treatment concentration for HGECs. The toothpaste sample with this concentration is selected, and the lipopolysaccharide (LPS) induced gingival epithelial cell HEGCs inflammation model (LPS induced model group) and normal gingival epithelial cells HEGCs (normal control group) are used to detect the contents of cytokines NO, IL-4, IL-10, IL-6, IL-1β, and TNF-α, and evaluate the anti-inflammatory effect of toothpaste.

[0113] The CCK-8 method was used to detect the proliferation and toxicity of the toothpaste samples on HGECs, and the results showed that the sample concentration of 0.6 ug / mL was the most suitable for evaluating the anti-inflammatory effect of each group of toothpaste samples.

[0114] After LPS induction, the cytokines NO, IL-4, IL-10, IL-6, IL-1β, TNF-α secreted by HGECs after incubation with each toothpaste sample, Figure 14 The determination of cytokines IL-4 and IL-10 secreted by HGECs after LPS induction incubation with each toothpaste sample; Figure 15 The determination of cytokines NO and IL-1β secreted by HGECs after LPS induction incubation with each toothpaste sample; Figure 16 The determination of cytokines IL-6 and TNF-α secreted by HGECs after LPS induction incubation with each toothpaste sample. Figure 14 、 Figure 15 、 Figure 16 It can be seen from Table 5, Table 6, Table 7 and Table 8 that each toothpaste sample can increase the content of anti-inflammatory factors (P<0.05 or P<0.01) and decrease the content of pro-inflammatory factors (P<0.05 or P<0.01) compared with the LPS-induced model group, but Example 5 has a better effect on increasing anti-inflammatory factor IL-4 and reducing IL-1β, TNF-α and NO (P<0.05). Figure 14 、 Figure 15 、 Figure 16 In Table 5, Table 6, Table 7 and Table 8, * indicates a significant difference (P<0.05) compared with the LPS-induced model group, ** indicates a significant difference (P<0.01) compared with the LPS-induced model group, and # indicates a significant difference (P<0.05) compared with the control group.

[0115] (3) Through the rat gingivitis model, the probe was used to detect the bleeding index (BOP), gingival index (GI), plaque index (PI), and the contents of cytokines NO, IL-4, IL-10, IL-6, IL-1β and TNF-α in rat plasma, and the in vivo experimental effect of toothpaste was evaluated.

[0116] SPF level SD rats, 56 in total, female, weight 200±20g, active, no caries, no periodontal disease. After intraperitoneal injection of sodium pentobarbital anesthesia, the second maxillary molar of the rats was ligated with medical silk thread at the neck of the tooth. The silk thread was put into the gingival sulcus as much as possible. After ligation, 10% sugar water was used instead of drinking water. The prepared bacterial liquid 0.5mL was inoculated every other day. The normal control group was the sham operation group, which was not ligated with silk thread and not fed with 10% sugar water after anesthesia. After the modeling was successful, the model rats were randomly divided into 7 groups. 6 groups were given the test sample for 30 days according to the experimental scheme. Another model group and normal control group (sham operation group) were only given normal saline. After intraperitoneal injection of sodium pentobarbital anesthesia, the rats were fixed, and the probe bleeding index (BOP), gingival index (GI), plaque index (PI), and the content of cytokines NO, IL-4, IL-10, IL-6, IL-1β, TNF-α in rat plasma were detected to evaluate the in vivo experimental effect of the toothpaste.

[0117] The gingival index GI was used to evaluate the health of the oral cavity by checking the color and quality changes of the gingiva. The plaque index PI was used to evaluate the prevention and treatment effect of periodontal disease in the oral cavity according to the thickness of the dental plaque. The gingival bleeding index BOP was used to evaluate the pathological condition of the oral gingiva by stimulating part of the gingiva with a probe. (The control group was the normal rats with sham operation, and the model rats were the rats with gingivitis.)

[0118] The results showed that compared with the rat gingivitis model, each toothpaste significantly reduced the rat oral GI, PI, and BOP (P<0.05). Among them, example 5 significantly reduced the GI, PI, and BOP levels compared with the comparative example (P<0.05), Figure 17 The effects of each toothpaste sample on the rat oral GI (gingival index), PI (plaque index), and BOP (bleeding index) were evaluated. The changes in inflammatory factors in serum showed that each toothpaste sample could increase the content of anti-inflammatory factors (P<0.05) and decrease the content of pro-inflammatory factors (P<0.05 or P<0.01) compared with the model group. However, example 5 had better effects on increasing anti-inflammatory factors IL-4 and IL-10 and decreasing IL-1β, TNF-α, and IL-6 (P<0.05), as shown in Figure 18 The effects of each toothpaste sample on the content of anti-inflammatory factors (IL-4, IL-10) were evaluated. Figure 19 The effects of each toothpaste sample on the content of pro-inflammatory factor IL-1β were evaluated. Figure 20 The effects of each toothpaste sample on the content of pro-inflammatory factor IL-6 were evaluated. Figure 21 The effects of each toothpaste sample on the content of pro-inflammatory factor TNF-α were evaluated. Figure 18 、 Figure 19 、 Figure 20 、 Figure 21In the table, * means significant difference (P<0.05) compared with the model group, ** means significant difference (P<0.01) compared with the model group, and # means significant difference (P<0.05) compared with the control group.

[0119] The above experimental results show that, through the above animal experiments, the anti-inflammatory and hemostatic effects of the six toothpastes can be ranked as follows: Example 5 (containing ginger-prepared Elsholtzia polystachya polysaccharide and Sarcandra polysaccharide) > Example 4 (containing ginger-prepared Elsholtzia polystachya polysaccharide) > Comparative Example 1 (containing Elsholtzia composition) > Comparative Example 2 (Yunnan Baiyao toothpaste) ≈ Comparative Example 3 (Sarcandra toothpaste) > Comparative Example 4 (Yunnan Tianqi toothpaste).

[0120] Among them, the toothpaste prepared in Example 5 (containing ginger-prepared Elsholtzia polystachya polysaccharide and Sarcandra polysaccharide) can significantly increase the platelet aggregation rate and the content of platelet-secreted TXB2 in the fresh blood of the white rabbits under the same dosage and concentration of the toothpaste samples, and is also higher than that of the toothpaste prepared in Example 4 (containing ginger-prepared Elsholtzia polystachya polysaccharide), indicating that the ginger-prepared Elsholtzia polystachya polysaccharide and the appropriate amount of Sarcandra polysaccharide have a synergistic effect, and the anti-inflammatory and hemostatic effect is better.

[0121] The rat gingivitis model experiment also proves that, compared with Comparative Examples 1-4 under the same conditions, the toothpaste prepared in Example 5 of the present application can significantly reduce the levels of GI (gingival index), PI (plaque index), and BOP (bleeding index), and has a better effect on improving the anti-inflammatory factors IL-4 and IL-10 and reducing the inflammatory factors IL-1β, TNF-α, and IL-6, and the effect is also better than that of the toothpaste prepared in Example 4, indicating that the ginger-prepared Elsholtzia polystachya polysaccharide and the appropriate amount of Sarcandra polysaccharide have a synergistic effect, and the anti-inflammatory and hemostatic effect is better.

[0122] The specific embodiments of the present application are described in detail above, but they are only examples, and the present application is not limited to the above described specific embodiments. Any equivalent modifications and substitutions made by those skilled in the art to the present application are also within the scope of the present application. Therefore, equivalent transformations and modifications made without departing from the spirit and scope of the present application are also included in the scope of the present application.

Claims

1. A traditional Chinese medicine toothpaste with anti-inflammatory and hemostatic effects, characterized in that: The toothpaste comprises 0.1-1% of the traditional Chinese medicine polysaccharide composition and 65-85% of the toothpaste raw material, and the rest is solvent; the traditional Chinese medicine polysaccharide composition comprises elsholtzia polystachya polysaccharide and corallium polysaccharide; the ratio of the elsholtzia polystachya polysaccharide and the corallium polysaccharide is 1:0.5-1 by mass fraction. ​ 2. The toothpaste with traditional Chinese medicine having anti-inflammatory and hemostatic efficacy according to claim 1, characterized in that: The toothpaste raw material comprises but is not limited to abrasive, thickening agent, humectant, foaming agent.

3. The toothpaste with traditional Chinese medicine having anti-inflammatory and hemostatic efficacy according to claim 1, characterized in that: The extraction method of the elsholtzia polystachya polysaccharide comprises the following steps: S1 preparation of ginger juice; S2 processing elsholtzia polystachya with ginger juice; S3 extraction of elsholtzia polystachya polysaccharide: the elsholtzia polystachya processed with ginger juice is pretreated, hot water extracted, reduced pressure concentrated, free protein removed by Sevag method, precipitated with ethanol, dried and ground to obtain elsholtzia polystachya polysaccharide.

4. The toothpaste with traditional Chinese medicine having anti-inflammatory and hemostatic efficacy according to claim 1, characterized in that: The extraction method of the corallium polysaccharide comprises the following steps: S10 taking corallium whole herb decoction pieces, crushing, adding distilled water, refluxing and extracting multiple times, and filtering; the combined extract is reduced pressure concentrated to obtain concentrated solution; S20 adding ethanol to the concentrated solution, and placing in a 4°C refrigerator for alcohol precipitation overnight; S30 discarding the supernatant, centrifuging the lower precipitate, collecting the bottom precipitate, freeze-drying the precipitate, and grinding the dried precipitate into powder to obtain corallium polysaccharide.

5. The toothpaste with traditional Chinese medicine having anti-inflammatory and hemostatic efficacy according to claim 3, characterized in that, In step S1, the preparation of ginger juice is as follows: fresh ginger is washed with clean water to remove the dirt on the outside of the ginger, and then sliced and dried; after the surface of the ginger slices is dried, distilled water is added and heated to decoct 2-4 times; the combined filtrate is reduced pressure concentrated to a crude drug mass concentration of 0.9-1.1 g / mL to obtain ginger juice; the mass-volume ratio of ginger to distilled water is 1:3-5.

6. The toothpaste with traditional Chinese medicine having anti-inflammatory and hemostatic efficacy according to claim 3, characterized in that, In step S2, the processing of elsholtzia polystachya with ginger juice is as follows: elsholtzia polystachya decoction pieces are placed in a sealed container, and an equal volume of ginger juice is added to soak in a cool place; after soaking, the elsholtzia polystachya is fried to obtain elsholtzia polystachya processed with ginger juice; the soaking time is 4-8 h; the frying time is 6-10 min.

7. The toothpaste with traditional Chinese medicine having anti-inflammatory and hemostatic efficacy according to claim 3, characterized in that, In step S3, the pretreatment is as follows: the elsholtzia polystachya processed with ginger juice is added with distilled water, and pretreated under ultrasonic extraction conditions; the mass-volume ratio of elsholtzia polystachya to distilled water is 1:15-25; the ultrasonic extraction time is 8-12 min, and the ultrasonic power is 80 w; in step S3, the hot water extraction and reduced pressure concentration are as follows: the pretreated elsholtzia polystachya is hot water extracted, and the obtained filtrate is reduced pressure concentrated to a crude drug mass concentration of 0.15-0.25 g / mL; the hot water extraction temperature is 85-95°C, and the hot water extraction time is 1-2 h; the reduced pressure concentration temperature is 52-60°C, and the reduced pressure concentration rotation speed is 40-60 rpm / min.

8. The toothpaste with traditional Chinese medicine having anti-inflammatory and hemostatic efficacy according to claim 3, characterized in that, The Sevag method for removing free proteins in step S3 is as follows: first, according to the volume ratio of Sevag reagent to concentrated solution of 1:4-6, the Sevag reagent is added to the concentrated solution, and after shaking and centrifugation, the supernatant is collected after the solution is stratified and centrifuged; second, according to the volume ratio of Sevag reagent to supernatant of 1:4-6, the Sevag reagent is added to the supernatant, and after shaking and centrifugation, the purified supernatant is obtained; the supernatant is treated repeatedly for 0-2 times; the Sevag reagent is prepared by mixing chloroform and n-butanol at a ratio of 4:1; the centrifugal speed is 2500-3500 rpm / min, and the centrifugal time is 4-8 min.

9. The toothpaste with traditional Chinese medicine having anti-inflammatory and hemostatic efficacy according to claim 3, characterized in that, The ethanol precipitation in step S3 is as follows: 92%-99% ethanol is added to the supernatant, and the solution is placed in a 4°C refrigerator for alcohol precipitation overnight; the volume ratio of the supernatant to 92%-99% ethanol is 1:2.5-3.5; the drying and grinding in step S3 is as follows: after alcohol precipitation overnight, the supernatant is removed, the bottom precipitate is collected by centrifugation, and then dried and ground into powder, thereby obtaining the ginger prepared moslae polysaccharide; the centrifugation is performed at 4500-5000 rpm / min for 8-12 min to collect the bottom precipitate; and the drying is freeze-drying.

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