Bactericidal composition and its application

Abstract

The present invention belongs to the field of agricultural fungicides, specifically a fungicide composition and its use. The composition comprises two active components, A and B, with the weight ratio of active component A to active component B being 1:99-99:1. Active component A is one or more pyrimidine-containing substituted pyrazole compounds or their salts, and active component B is selected from a fungicide. The fungicide composition of the present invention is particularly suitable for controlling a variety of plant pathogenic fungal and bacterial diseases.

Description

[0001] This application is a further divisional application of the divisional application 202210553242.8 of the Chinese invention patent 202011314120.0; the application date of the Chinese invention patent 202011314120.0 is November 20, 2020, the name of the invention is: bactericidal composition and its application, publication number: CN112825859A, the publication number of the Chinese invention patent 202210553242.8 is: CN114903046A; because the application of the Chinese invention patent 202210553242.8 has the unity problem pointed out by the examiner, the applicant again filed a divisional application for the Chinese invention patent 202210553242.8. Technical Field

[0002] The present invention belongs to the field of agricultural fungicides, and particularly relates to a fungicide composition of a pyrimidine-containing substituted pyrazole compound and a fungicide, and application thereof. Background Art

[0003] Patent WO2016184378 discloses a pyrimidine-containing substituted pyrazole compound and its use, wherein it is reported that the compounds represented by the following general formulas IA and IB have good activity against various diseases.

[0004]

[0005] Respiratory inhibitors, nucleic acid metabolism inhibitors, cell wall synthesis inhibitors, signal transduction inhibitors, cytoskeleton and motor protein inhibitors, sterol biosynthesis inhibitors, phospholipid synthesis inhibitors, cell wall synthesis inhibitors, and methionine biosynthesis inhibitors are widely used in agricultural production and have outstanding control effects. However, due to their single site of action, the field efficacy of some varieties has significantly decreased after long-term repeated use. Multi-site inhibitor fungicides are a broad-spectrum protective fungicide, but they have poor therapeutic activity and require large field dosages.

[0006] In the practical application of fungicides, it is often found that a given agent exhibits excellent disease control effects initially, but as use increases and dosages are gradually increased, efficacy improves minimally, or sometimes even completely loses its effectiveness. This is primarily due to changes in the pathogen population: some pathogens acquire drug-resistant plasmids through mutation, becoming resistant and gradually forming dominant populations through natural selection, leading to reduced fungicide efficacy. Furthermore, it is also recognized that after pesticide application, some particles are dispersed onto the soil, dissipated into the air, or carried into rivers and lakes with rainwater and farmland drainage, causing environmental pollution. Therefore, there is an urgent need for pesticides that offer high efficacy, require low dosages, minimize environmental pollution, and can slow the development of pathogen resistance.

[0007] The combined use of pesticides is an important means of delaying the development of resistance in pathogens and an effective method for reducing pesticide dosage. The scientific validity of combined fungicide applications depends not only on the target and mechanism of action, but also on the nature of the combined action of the mixed agents. Therefore, the development of highly effective fungicide combinations with complementary and synergistic effects is subject to numerous constraints, resulting in a very low success rate. Summary of the Invention

[0008] The present invention aims to provide a synergistic fungicidal composition of a substituted pyrazole compound containing pyrimidine and a fungicide and its use.

[0009] To achieve the above purpose, the present invention adopts the following technical solutions:

[0010] A bactericidal composition comprising two active components, A and B, wherein the weight ratio of active component A to active component B is 1:99-99:1;

[0011] The active component A is one or more pyrimidine-containing substituted pyrazole compounds or salts thereof; and the active component B is selected from a fungicide.

[0012] The active component A is a compound represented by the general formula IA or IB or a salt thereof:

[0013]

[0014] Where:

[0015] R1 is selected from hydrogen, halogen, cyano, nitro, amino, carboxyl, C1-C4 alkyl, halogenated C1-C4 alkyl, C3-C4 cycloalkyl, C1-C4 alkoxy, halogenated C1-C4 alkoxy, C1-C4 alkylthio, halogenated C1-C4 alkylthio, C1-C4 alkylsulfinyl, C1-C4 alkylsulfonyl, C2-C4 alkenyl, halogenated C2-C4 alkenyl, C2-C4 alkynyl, halogenated C2-C4 alkynyl , C3-C4 alkenyloxy, halogenated C3-C4 alkenyloxy, C3-C4 alkynyloxy, halogenated C3-C4 alkynyloxy, C1-C4 alkylamino, di(C1-C4 alkyl)amino, C1-C4 alkylaminocarbonyl, halogenated C1-C4 alkylaminocarbonyl, C1-C4 alkoxycarbonyl, halogenated C1-C4 alkoxycarbonyl, C1-C4 alkoxyC1-C4 alkyl or C1-C4 alkylthioC1-C4 alkyl;

[0016] R2 is selected from hydrogen, halogen, cyano, nitro, amino, carboxyl, formyl, C1-C4 alkyl, halo-C1-C4 alkyl, C1-C4 alkoxy or halo-C1-C4 alkoxy;

[0017] R3 is selected from hydrogen, hydroxy, formyl, C1-C4 alkyl, halogenated C1-C4 alkyl, C1-C4 alkoxy, halogenated C1-C4 alkoxy, C3-C4 cycloalkyl, C1-C4 alkylthio, C2-C4 alkenylthio, C2-C4 alkenyl, C2-C4 alkynyl, halogenated C2-C4 alkenyl, halogenated C2-C4 alkynyl, C1-C4 alkoxy C1-C4 alkyl, halogenated C1-C4 alkoxy C1-C4 C1-C4 alkyl, C1-C4 alkylthio C1-C4 alkyl, halogenated C1-C4 alkylthio C1-C4 alkyl, C1-C4 alkylsulfinyl, halogenated C1-C4 alkylsulfinyl, C1-C4 alkylsulfonyl, halogenated C1-C4 alkylsulfonyl, C1-C4 alkylaminosulfonyl, di(C1-C4 alkyl)aminosulfonyl, C1-C4 alkylsulfonylaminocarbonyl, C1-C4 alkylcarbonylaminosulfonyl C3-C4 cycloalkyloxycarbonyl, C1-C4 alkylcarbonyl, halogenated C1-C4 alkylcarbonyl, C1-C4 alkoxycarbonyl, halogenated C1-C4 alkoxycarbonyl, C1-C4 alkylcarbonyl C1-C4 alkyl, C1-C4 alkoxycarbonyl C1-C4 alkyl, C1-C4 alkylaminocarbonyl, di(C1-C4 alkyl)aminocarbonyl, C2-C4 alkenyloxycarbonyl, C2-C4 alkynyloxycarbonyl, C1-C4 alkoxy C1-C4 alkoxycarbonyl, C1-C4 alkylaminothio, di(C1-C4 alkyl)aminothio, arylcarbonyl C1-C4 alkyl, arylcarbonyl, aryloxycarbonyl, aryl C1-C4 alkyloxycarbonyl, aryl C1-C4 alkyl, heteroarylcarbonyl C1-C4 alkyl, heteroarylcarbonyl, heteroaryloxycarbonyl, heteroaryl C1-C4 alkyloxycarbonyl or heteroaryl C1-C4 alkyl;

[0018] R4 and R5 may be the same or different and are selected from hydrogen, halogen, C1-C4 alkyl, halogenated C1-C4 alkyl, C1-C4 alkoxy or halogenated C1-C4 alkoxy; wherein R4, R5 and the C to which they are connected may also form a C3-C4 ring;

[0019] R6 and R7 may be the same or different and are selected from hydrogen, halogen, C1-C4 alkyl, halogenated C1-C4 alkyl, C1-C4 alkoxy or halogenated C1-C4 alkoxy; wherein R6, R7 and the C to which they are connected may also form a C3-C4 ring;

[0020] R8 and R9 may be the same or different and are selected from hydrogen, cyano, halogen, C1-C4 alkyl, halogenated C1-C4 alkyl, C1-C4 alkoxycarbonyl, halogenated C1-C4 alkoxycarbonyl, unsubstituted or substituted with 1-5 R 11 substituted aryl, arylmethyl, arylcarbonyl, arylmethylcarbonyl, aryloxycarbonyl, heteroaryl, heteroarylmethyl, heteroarylcarbonyl, heteroarylmethylcarbonyl, or heteroaryloxycarbonyl;

[0021] R11 is selected from the group consisting of halogen, hydroxy, amino, cyano, nitro, C1-C4 alkyl, halogenated C1-C4 alkyl, C1-C4 alkoxy, halogenated C1-C4 alkoxy, C3-C4 cycloalkyl, C1-C4 alkylamino, halogenated C1-C4 alkylamino, di(C1-C4 alkyl)amino, halogenated di(C1-C4 alkyl)amino, C1-C4 alkylthio, halogenated C1-C4 alkylthio, C2-C4 alkenyl, C2-C4 alkynyl, C2-C4 alkenyloxy, halogenated C2-C4 alkenyloxy, C2-C4 alkynyloxy, halogenated C2-C4 alkynyloxy, C1-C4 alkylsulfonyl, halogenated C1-C4 alkylsulfonyl, C1-C4 alkylcarbonyl, halogenated C1-C4 alkylcarbonyl, C1-C4 alkoxycarbonyl, halogenated C1-C4 alkoxycarbonyl, C1-C4 alkoxyC1-C4 -C4 alkyl, halogenated C1-C4 alkoxy C1-C4 alkyl, C1-C4 alkylthio C1-C4 alkyl, halogenated C1-C4 alkylthio C1-C4 alkyl, C1-C4 alkoxycarbonyl C1-C4 alkyl, halogenated C1-C4 alkoxycarbonyl C1-C4 alkyl, C1-C4 alkylthiocarbonyl C1-C4 alkyl, halogenated C1-C4 alkylthiocarbonyl C1-C4 alkyl, C1-C4 alkylcarbonyloxy, halogenated C1-C4 alkylcarbonyloxy, C1-C4 alkoxycarbonyloxy, halogenated C1-C4 alkoxycarbonyloxy, C1-C4 alkylsulfonyloxy, halogenated C1-C4 alkylsulfonyloxy, C1-C4 alkoxy C1-C4 alkoxy or halogenated C1-C4 alkoxy C1-C4 alkoxy; n is an integer selected from 0 to 5, when n is greater than 1, R 11 Can be the same or different;

[0022] R 14 、R 15 、R 16 or R 17 may be the same or different and are independently selected from hydrogen, halogen, hydroxy, amino, cyano, nitro, C1-C4 alkyl, halo-C1-C4 alkyl, C1-C4 alkoxy, halo-C1-C4 alkoxy or C3-C4 cycloalkyl;

[0023] W is selected from hydrogen, halogen, C1-C4 alkyl, halogenated C1-C4 alkyl, C3-C4 cycloalkyl, C1-C4 alkoxy, C1-C4 alkylthio or C1-C4 alkylsulfonyl;

[0024] The salt of the pyrimidine-containing substituted pyrazole compound is a salt formed by the compound represented by the general formula IA or IB and hydrochloric acid, sulfuric acid, phosphoric acid, formic acid, acetic acid, trifluoroacetic acid, oxalic acid, methanesulfonic acid, p-toluenesulfonic acid, benzoic acid, phthalic acid, maleic acid, fumaric acid, sorbic acid, malic acid or citric acid;

[0025] The active component B is selected from one or more of respiratory inhibitor fungicides, nucleic acid metabolism inhibitor fungicides, cell wall synthesis inhibitor fungicides, signal transduction inhibitor fungicides, cytoskeleton and motor protein inhibitor fungicides, sterol biosynthesis inhibitor fungicides, phospholipid synthesis inhibitor fungicides, methionine biosynthesis inhibitor fungicides, multi-site inhibitor fungicides, and fungicides with unknown mechanisms of action.

[0026] When the active component A is a compound of formula IA or a salt thereof, preferably, the composition comprises the active component A and the active component B in a weight ratio of 1:50 to 50:1;

[0027] Wherein, the active component A is selected from one or more of the compounds of formula IA or their salts;

[0028] wherein: R3 is selected from hydrogen, hydroxy, formyl, C1-C4 alkyl, halo-C1-C4 alkyl, C1-C4 alkoxy, halo-C1-C4 alkoxy, C3-C4 cycloalkyl, C1-C4 alkylthio, C2-C4 alkenylthio, C2-C4 alkenyl, C2-C4 alkynyl, halo-C2-C4 alkenyl or halo-C2-C4 alkynyl;

[0029] R4 and R5 may be the same or different and are independently selected from hydrogen, halogen, C1-C4 alkyl, halo-C1-C4 alkyl, C1-C4 alkoxy or halo-C1-C4 alkoxy; R6 and R7 may be the same or different and are independently selected from hydrogen, halogen, C1-C4 alkyl, halo-C1-C4 alkyl, C1-C4 alkoxy or halo-C1-C4 alkoxy; R8 and R9 may be the same or different and are independently selected from hydrogen, cyano, halogen, C1-C4 alkyl, halo-C1-C4 alkyl, C1-C4 alkoxycarbonyl or halo-C1-C4 alkoxycarbonyl; R 11 is selected from halogen, hydroxy, amino, cyano, nitro, C1-C4 alkyl, halogenated C1-C4 alkyl, C1-C4 alkoxy, halogenated C1-C4 alkoxy, C3-C4 cycloalkyl, C1-C4 alkylamino, halogenated C1-C4 alkylamino, di(C1-C4 alkyl)amino, halogenated di(C1-C4 alkyl)amino or C1-C4 alkylthio; n is an integer selected from 0 to 5, when n is 0, there is no substituent on the phenyl ring; when n is greater than 1, R 11 Can be the same or different; R 14 、R 15 、R 16 or R 17 may be the same or different and are independently selected from hydrogen, halogen, hydroxy, amino, cyano, nitro, C1-C4 alkyl, halo-C1-C4 alkyl or C1-C4 alkoxy; W is selected from hydrogen, halogen, C1-C4 alkyl, halo-C1-C4 alkyl, C3-C4 cycloalkyl, C1-C4 alkoxy or C1-C4 alkylthio;

[0030] The salt of the compound represented by the general formula IA is a salt formed by the compound of the general formula with hydrochloric acid, sulfuric acid, phosphoric acid, formic acid, acetic acid, trifluoroacetic acid, oxalic acid, methanesulfonic acid, p-toluenesulfonic acid, benzoic acid, phthalic acid or maleic acid;

[0031] The active component B is selected from flumorph B41, dimethomorph B42, mandipropamid B43, zoxamide B44, metalaxyl B45, metalaxyl-M B46, iprovalicarb B47, cymoxanil B48, benalaxyl-M B49, flusulfamide B50, tiadinil B51, ethaboxam B52, cyflufenamid B53, fenhexamid B54, diclocymet B55, bupirimate B56, dimethirimol B57, ethirimol B58, hymexazol B59, octhilinone B60, ofurace B61, chinomethionat B62, metidaxyl B63, furaxyl B64, fludioxonil B65, fenpicloni B66, iprodione B67, procymidone B68, meclozolin B69, chlozolinate B70, dimethachlon B71, vinclozolin B72, diethofencarb B73, metrafenone B74, pyriofenoneB75, carbendazim B76, thiophanate-methyl B77, phenamacril B78, benomyl B79, fuberidazole B80, thiabendazole B81, thiophanate B82, pencycuron B83, etaconazole B84, ethanone B85, fenarimol B86, fenbuconazole B87, fenpropidine B88, fenpropimorph B89, fenpyrazamine B90, mefeconazole ntrifluconazole B91, nuarimol B92, oxpoconazole B93, pefurazoate B94, prochloraz B95, spiroxamine B96, pyrisoxazole B97, tridemorph B98, triforine B99, dodemorph B100, bromuconazole B101, triapenthenol B102, naftifine B103, simeconazole B104, triflumizole B105, ipfentrifluconazole B106, Tetrachloronitrobenzene (tecnazene) B107, Chloroneb B108, Dicloran B109, Biphenyl B110, Edifenphos B111, Etridiazole B112, Iodocarb B113, Iprobenfos B114, Isoprothiolane B115, Oxathiapiprolin B116, FluoxapiprolineB117, propamocarb B118, prothiocarb B119, pyrazophos B120, quintozene B121, tolclofos-methyl B122, benthiavalicarb-isopropyl B123, polyoxin B124, valifenalate B125, pyrimorph B12 6. Blasticidin-s B127, cyprodinil B128, kasugamycin B129, mepanipyrim B130, pyrimethanil B131, streptomycin B132, oxytetracycline B133, zineb B134, mancozeb B135, metiram B136, propineb propineb B137, maneb B138, amobam B139, nabam B140, etem B141, milneb B142, mancopper B143, cufraneb B144, sulfur B145, anilazine B146, captan B147, chinomethionat B148, chlorothalonil othalonil B149, dichlofluanid B150, tolylfluanid B151, dithianon B152, fluoroimide B153, methasulfocarb B154, captafol B155, folpet B156, iminoctadine B157, guazatine B158, zinc thiazole B159, oxine-copper B160, thiodiazole-copper B161, cuppric nonyl phenolsulfonate B162, picarbutrazox B163, aminopyrifenOne or more of: B164, metyltetraprole B165, dodine B166, pyridachlometyl B167, diclomezine B168, validamycin B169, ferimzone B170, tebufloquin B171, triazoxide B172, quinofumelin B173, ipflufenoquin B174, dipymetitrone B175, and teclofthalam B176.

[0032] Further preferably, the composition comprises active component A and active component B in a weight ratio of 1:20-20:1;

[0033] Wherein, the active component A is one or more of the compounds represented by the general formula IA or their salts;

[0034] wherein R3 is selected from hydrogen, hydroxy, formyl, C1-C4 alkyl, halo-C1-C4 alkyl, C1-C4 alkoxy, halo-C1-C4 alkoxy, C3-C4 cycloalkyl, C1-C4 alkylthio, C2-C4 alkenylthio, C2-C4 alkenyl, C2-C4 alkynyl, halo-C2-C4 alkenyl or halo-C2-C4 alkynyl;

[0035] R4 and R5 may be the same or different and are independently selected from hydrogen, halogen, C1-C4 alkyl, halo-C1-C4 alkyl, C1-C4 alkoxy or halo-C1-C4 alkoxy; R6 and R7 may be the same or different and are independently selected from hydrogen, halogen, C1-C4 alkyl, halo-C1-C4 alkyl, C1-C4 alkoxy or halo-C1-C4 alkoxy; R8 and R9 may be the same or different and are independently selected from hydrogen, cyano, halogen, C1-C4 alkyl, halo-C1-C4 alkyl, C1-C4 alkoxycarbonyl or halo-C1-C4 alkoxycarbonyl; R 11 is selected from halogen, hydroxy, amino, cyano, nitro, C1-C4 alkyl, halogenated C1-C4 alkyl, C1-C4 alkoxy, halogenated C1-C4 alkoxy, C3-C4 cycloalkyl, C1-C4 alkylamino or halogenated C1-C4 alkylamino; n is an integer selected from 0 to 5, when n is 0, there is no substituent on the phenyl ring; when n is greater than 1, R 11 Can be the same or different; R 14 、R 15 、R 16 or R 17may be the same or different and are independently selected from hydrogen, halogen, hydroxy, amino, cyano, nitro, C1-C4 alkyl, halo-C1-C4 alkyl, C1-C4 alkoxy or halo-C1-C4 alkoxy; W is selected from hydrogen, halogen, C1-C4 alkyl, halo-C1-C4 alkyl, C3-C4 cycloalkyl, C1-C4 alkoxy or C1-C4 alkylthio;

[0036] The salt of the compound represented by the general formula IA is a salt formed by the compound of the general formula with hydrochloric acid, sulfuric acid, phosphoric acid, formic acid, acetic acid, trifluoroacetic acid, oxalic acid or methanesulfonic acid;

[0037] The active component B is selected from flumorph B41, dimethomorph B42, mandipropamid B43, zoxamide B44, metalaxyl-M B46, iprovalicarb B47, cymoxanil B48, benalaxyl-M B49, tiadinil B51, ethaboxam B52, bupirimate B56, dimethirimol B57, ethi rimol B58, hymexazol B59, octhilinone B60, ofurace B61, chinomethionat B62, fludioxonil B65, fenpicloni B66, iprodione B67, procymidone B68, dimethachlon B71, vinclozolin B72, diethofencarb B73, metrafenone B74, pyriofenone B75, carbendazim, B76, thiophanate-methyl, B77, phenamacril, B78, benomyl, B79, ethanone, B85, fenarimol, B86, fenbuconazole, B87, fenpropidine, B88, fenpyrazamine, B90, mefentrifluconazole, B91, nuarimol, B92, oxpoconazole, B93, pefurazoate, B94, prochloraz, B95, spiroxamine, B96, pyrisoxazole, B97, simeconazole, B104, ipfentrifluconazoleB106, edifenphos B111, iprobenfos B114, isoprothiolane B115, oxathiapiprolin B116, fluoxapiproline B117, propamocarb B118, prothiocarb B119, pyrazophos B120, quintozene B121, tolclofos-methyl B122, benthiavalicarb-isopropyl B123, polyoxin B124, valifenalate B125, pyrimorph B126, cyprodinil B128, kasugamycin B129, mepanipyrim B130, pyrimethanil B131, streptomycin B132, Zineb B134, mancozeb B135, metiram B136, propineb B137, maneb B138, amobam B139, milneb B142, mancopper B143, sulfur B145, anilazine B146, captan B147, chlorothalonil B149, dichlofluanid B150, tolylfluanid B151, methasulfocarb B154, captafol B155, folpet B156, zinc thiazole thiazole B159, oxine-copper B160, thiodiazole-copper B161, cuppric nonylphenolsulfonate B162, picarbutrazox B163, aminopyrifen B164, metyltetraprole B165, pyridachlometyl B167, validamycin B169, ferimzone B170, tebufloquinOne or more of B171, quinofumelin B173, ipflufenoquin B174, dipymetitrone B175, and teclofthalam B176.

[0038] Still further preferably, the composition comprises active component A and active component B in a weight ratio of 1:10-10:1;

[0039] Wherein, the active component A is selected from one or more of the compounds of formula IA or their salts;

[0040] wherein R3 is selected from hydrogen, hydroxy, formyl or C1-C4 alkyl; R4 and R5 may be the same or different and are selected from hydrogen, halogen or C1-C4 alkyl; R6 and R7 may be the same or different and are selected from hydrogen, halogen or C1-C4 alkyl; R8 and R9 may be the same or different and are selected from hydrogen, cyano, halogen or C1-C4 alkyl; R 11 is selected from halogen, hydroxyl, amino, cyano, nitro or C1-C4 alkyl; n is selected from an integer from 0 to 5, when n is 0, there is no substituent on the benzene ring; when n is greater than 1, R 11 Can be the same or different; R 14 、R 15 、R 16 or R 17 may be the same or different and are independently selected from hydrogen, halogen, hydroxy, amino, cyano, nitro or C1-C4 alkyl; W is selected from hydrogen, halogen or C1-C4 alkyl;

[0041] The salt of the compound represented by the general formula IA is a salt formed by the compound of the general formula with hydrochloric acid, sulfuric acid, phosphoric acid, formic acid or acetic acid;

[0042] Active ingredient B is selected from flumorph B41, dimethomorph B42, mandipropamid B43, zoxamide B44, metalaxyl-M B46, iprovalicarb B47, cymoxanil B48, tiadinil B51, ethaboxam B52, bupirimid B53, thiazolinone B54, thiazolinone B55, thiazolinone B56, thiazolinone B57, thiazolinone B58, thiazolinone B59, thiazolinone B60, thiazolinone B61, thiazolinone B62, thiazolinone B63, thiazolinone B64, thiazolinone B65, thiazolinone B66, thiazolinone B67, thiazolinone B68, thiazolinone B69, thiazolinone B70, thiazolinone B71, thiazolinone B72, thiazolinone B73, thiazolinone B74, thiazolinone B75, thiazolinone B76, thiazolinone B77, thiazolinone B78, thiazolinone B79, thiazolinone B80, thiazolinone B81, thiazolinone B82, thiazolinone B83, thiazolinone B84, thiazolinone B85, thiazolinone B86, thiazolinone B87, thiazolinone B88, thiazolinone B89, thiazolinone B90, thiazolinone B91, mate B56, ethirimol B58, hymexazol B59, octhilinone B60, chinomethionat B62, fludioxonil B65, iprodione B67, procymidone B68, diethofencarb B73, metrafenone B74, pyriofenone B75, carbendazim, B76, thiophanate-methyl, B77, phenamacril, B78, fenbuconazole, B87, mefentrifluconazole, B91, nuarimol, B92, prochloraz, B95, spiroxamine, B96, pyrisoxazole, B97, simeconazole, B104, ipfentrifluconazoleB106, iprobenfos B114, isoprothiolane B115, oxathiapiprolin B116, fluoxapiproline B117, propamocarb B118, quintozene B121, tolclofos-methyl B122, benthiavalicarb-isopropyl B123, polyoxin B124, valifenalate B125, pyrimorph B126, cyprodinil B128, kasugamycin B129, Pyrimethanil B131, streptomycin B132, zineb B134, mancozeb B135, metiram B136, propineb B137, sulphur B145, captan B147, chlorothalonil B149, dichlofluanid B150, captafol B155, folpet B156, zinc thiazole B159, oxine-copper B160, thiodiazole-copper B161, cupric one or more of: oxadiazine B164, dapoxetine B165, dapoxetine B166, dapoxetine B167, dapoxetine B168, dapoxetine B169, dapoxetine B170, dapoxetine B171, dapoxetine B172, dapoxetine B173, dapoxetine B174, dapoxetine B175, dapoxetine B176, dapoxetine B177, dapoxetine B178, dapoxetine B179, dapoxetine B180, dapoxetine B181, dapoxetine B182, dapoxetine B183, dapoxetine B184, dapoxetine B185, dapoxetine B18

[0043] When the active component A is a compound of formula IB or a salt thereof, preferably, the composition comprises the active component A and the active component B in a weight ratio of 1:50 to 50:1;

[0044] Wherein, the active component A is selected from one or more of the compounds of formula IB or their salts;

[0045] wherein R1 is selected from hydrogen, halogen, cyano, nitro, amino, carboxyl, C1-C4 alkyl, halogenated C1-C4 alkyl, C3-C4 cycloalkyl, C1-C4 alkoxy, halogenated C1-C4 alkoxy, C1-C4 alkylthio, halogenated C1-C4 alkylthio, C1-C4 alkylsulfinyl, C1-C4 alkylsulfonyl, C2-C4 alkenyl, halogenated C2-C4 alkenyl, C2-C4 alkynyl or halogenated C2-C4 alkynyl; R2 is selected from hydrogen, halogen, cyano, nitro, amino, carboxyl, formyl, C1-C4 alkyl or halogenated C1-C4 alkyl; R3 is selected from hydrogen, hydroxy, formyl, C1-C4 alkyl, halogenated C1-C4 alkyl, C1-C4 alkoxy, halogenated C1-C4 alkoxy, C3-C4 alkylsulfonyl, C2-C4 alkenyl, halogenated C2-C4 alkenyl, C2-C4 alkynyl or halogenated C2-C4 alkynyl. -C4 cycloalkyl, C1-C4 alkylthio, C2-C4 alkenylthio, C2-C4 alkenyl, C2-C4 alkynyl, halogenated C2-C4 alkenyl or halogenated C2-C4 alkynyl; R4 and R5 may be the same or different and are independently selected from hydrogen, halogen, C1-C4 alkyl, halogenated C1-C4 alkyl, C1-C4 alkoxy or halogenated C1-C4 alkoxy; R6 and R7 may be the same or different and are independently selected from hydrogen, halogen, C1-C4 alkyl, halogenated C1-C4 alkyl, C1-C4 alkoxy or halogenated C1-C4 alkoxy; R8 and R9 may be the same or different and are independently selected from hydrogen, cyano, halogen, C1-C4 alkyl, halogenated C1-C4 alkyl, C1-C4 alkoxycarbonyl or halogenated C1-C4 alkoxycarbonyl; R 11 is selected from halogen, hydroxy, amino, cyano, nitro, C1-C4 alkyl, halogenated C1-C4 alkyl, C1-C4 alkoxy, halogenated C1-C4 alkoxy, C3-C4 cycloalkyl, C1-C4 alkylamino, halogenated C1-C4 alkylamino, di(C1-C4 alkyl)amino, halogenated di(C1-C4 alkyl)amino or C1-C4 alkylthio; n is an integer selected from 0 to 5, when n is 0, there is no substituent on the phenyl ring; when n is greater than 1, R 11 May be the same or different; W is selected from hydrogen, halogen, C1-C4 alkyl, halo C1-C4 alkyl, C3-C4 cycloalkyl, C1-C4 alkoxy or C1-C4 alkylthio;

[0046] The salt of the compound represented by the general formula IB is a salt formed by the compound of the general formula with hydrochloric acid, sulfuric acid, phosphoric acid, formic acid, acetic acid, trifluoroacetic acid, oxalic acid, methanesulfonic acid, p-toluenesulfonic acid, benzoic acid, phthalic acid or maleic acid;

[0047] Active ingredient B is selected from dinocap B40, flumorph B41, dimethomorph B42, mandipropamid B43, zoxamide B44, metalaxyl B45, metalaxyl-M B46, iprovalicarb B47, cymoxanil ) B48, benalaxyl-M B49, flusulfamide B50, tiadinil B51, ethaboxam B52, cyflufenamid B53, fenhexamid B54, diclocymet B55, bupirimate B56, dimethicone thirimol B57, ethirimol B58, hymexazol B59, octhilinone B60, ofurace B61, chinomethionat B62, metidaxyl B63, furaxyl B64, fludioxonil B65, fenpicloni B66, iprodione B67, procymidone B68, meclozolin B69, chlozolinate B70, dimethachlon B71, vinclozolin B72, diethofencarb B73, metrafenone B74, pyriofenoneB75, carbendazim B76, thiophanate-methyl B77, phenamacril B78, benomyl B79, fuberidazole B80, thiabendazole B81, thiophanate B82, pencycuron B83, etaconazole B84, ethanone B85, fenarimol B86, fenbuconazole B87, fenpropidine B88, fenpropimorph B89, fenpyrazamine B90, mefeconazole ntrifluconazole B91, nuarimol B92, oxpoconazole B93, pefurazoate B94, prochloraz B95, spiroxamine B96, pyrisoxazole B97, tridemorph B98, triforine B99, dodemorph B100, bromuconazole B101, triapenthenol B102, naftifine B103, simeconazole B104, triflumizole B105, ipfentrifluconazole B106, Tetrachloronitrobenzene (tecnazene) B107, Chloroneb B108, Dicloran B109, Biphenyl B110, Edifenphos B111, Etridiazole B112, Iodocarb B113, Iprobenfos B114, Isoprothiolane B115, Oxathiapiprolin B116, FluoxapiprolineB117, propamocarb B118, prothiocarb B119, pyrazophos B120, quintozene B121, tolclofos-methyl B122, benthiavalicarb-isopropyl B123, polyoxin B124, valifenalate B125, pyrimorph B12 6. Blasticidin-s B127, cyprodinil B128, kasugamycin B129, mepanipyrim B130, pyrimethanil B131, streptomycin B132, oxytetracycline B133, zineb B134, mancozeb B135, metiram B136, propineb propineb B137, maneb B138, amobam B139, nabam B140, etem B141, milneb B142, mancopper B143, cufraneb B144, sulfur B145, anilazine B146, captan B147, chinomethionat B148, chlorothalonil othalonil B149, dichlofluanid B150, tolylfluanid B151, dithianon B152, fluoroimide B153, methasulfocarb B154, captafol B155, folpet B156, iminoctadine B157, guazatine B158, zinc thiazole B159, oxine-copper B160, thiodiazole-copper B161, cuppric nonyl phenolsulfonate B162, picarbutrazox B163, aminopyrifenOne or more of: B164, metyltetraprole B165, dodine B166, pyridachlometyl B167, diclomezine B168, validamycin B169, ferimzone B170, tebufloquin B171, triazoxide B172, quinofumelin B173, ipflufenoquin B174, dipymetitrone B175, and teclofthalam B176.

[0048] Further preferably, the composition comprises active component A and active component B in a weight ratio of 1:20-20:1;

[0049] Wherein, the active component A is selected from one or more of the compounds of formula IB or their salts;

[0050] wherein R1 is selected from hydrogen, halogen, cyano, nitro, amino, carboxyl, C1-C4 alkyl or halogenated C1-C4 alkyl; R2 is selected from hydrogen, halogen, cyano, nitro, amino, carboxyl, formyl, C1-C4 alkyl or halogenated C1-C4 alkyl; R3 is selected from hydrogen, hydroxyl, formyl, C1-C4 alkyl, halogenated C1-C4 alkyl, C1-C4 alkoxy, halogenated C1-C4 alkoxy, C3-C4 cycloalkyl, C1-C4 alkylthio, C2-C4 alkenylthio, C2-C4 alkenyl, C2-C4 alkynyl, halogenated C2-C4 alkenyl or halogenated C2-C4 alkynyl; R4 and R5 may be the same or different and are independently selected from hydrogen, halogen, C1-C4 alkyl, halo-C1-C4 alkyl, C1-C4 alkoxy or halo-C1-C4 alkoxy; R6 and R7 may be the same or different and are independently selected from hydrogen, halogen, C1-C4 alkyl, halo-C1-C4 alkyl, C1-C4 alkoxy or halo-C1-C4 alkoxy; R8 and R9 may be the same or different and are independently selected from hydrogen, cyano, halogen, C1-C4 alkyl, halo-C1-C4 alkyl, C1-C4 alkoxycarbonyl or halo-C1-C4 alkoxycarbonyl; R 11 is selected from halogen, hydroxy, amino, cyano, nitro, C1-C4 alkyl, halogenated C1-C4 alkyl, C1-C4 alkoxy, halogenated C1-C4 alkoxy, C3-C4 cycloalkyl, C1-C4 alkylamino or halogenated C1-C4 alkylamino; n is an integer selected from 0 to 5, when n is 0, there is no substituent on the phenyl ring; when n is greater than 1, R 11 May be the same or different; W is selected from hydrogen, halogen, C1-C4 alkyl, halo C1-C4 alkyl, C3-C4 cycloalkyl, C1-C4 alkoxy or C1-C4 alkylthio;

[0051] The salt of the compound represented by the general formula IB is a salt formed by the compound of the general formula with hydrochloric acid, sulfuric acid, phosphoric acid, formic acid, acetic acid, trifluoroacetic acid, oxalic acid or methanesulfonic acid;

[0052] The active component B is selected from benzovindiflupyr B1, penflufen B2, isopyrazam B3, fluxapyroxad B4, fluopyram B5, flubeneteram B6, sedaxane B7, penthiopyrad B8, boscalid B9, oxadiazol B10, oxadiazol B11, oxadiazol B12, oxadiazol B13, oxadiazol B14, oxadiazol B15, oxadiazol B16, oxadiazol B17, oxadiazol B18, oxadiazol B19, oxadiazol B20, oxadiazol B21, oxadiazol B22, oxadiazol B23, oxadiazol B24, oxadiazol B25, oxadiazol B26, oxadiazol B27, oxadiazol B28, oxadiazol B29, oxadiazol B30, oxadiazol B31, oxadiazol B32, oxadiazol B33, oxadiazol B34, oxadiazol B35, oxadiazol B36, oxadiazol B37, oxadiazol B38, oxadiazol B39, oxadiazol B40, oxadiazol B41, oxadiazol B42, oxadiazol B43, oxadiazol B44, oxadiazol B45, oxadiazol B46, oxadiazol B47, oxadiazol B48, oxadiazol B49, oxadiazol B50, oxadiazol B51 B9, bixafen B10, flutolanil B11, furametpyr B12, thifluzamide B13, pydiflumetofen B14, fluindapyr B15, fluopimomide B16, isofetamido B17, inpyrfluxam B18, isoflurane B19, pyraziflumid B20, pyrapropoyne B21, benodanil B26, fluopicolide B27, fenamidone B28, famoxadone B29, cyazofamid B30, ametoctradin B31, fluazinam B32, fenpicoxamid B33, florylpicoxamidB34, amisulbrom B35, silthiopham B36, pyribencarb B37, flumorph B41, dimethomorph B42, mandipropamid B43, zoxamide B44, metalaxyl-M B46, iprovalicarb B47, cymoxanil B48, benalaxyl-M B49, tiadin il) B51, ethaboxam B52, bupirimate B56, dimethirimol B57, ethirimol B58, hymexazol B59, octhilinone B60, ofuramide B61, chinomethionat B62, fludioxonil B65, fenpicloni B66, iprodione B67, procymidone B68, Dimethachlon B71, vinclozolin B72, diethofencarb B73, metrafenone B74, pyriofenone B75, carbendazim B76, thiophanate-methyl B77, phenamacril B78, benomyl B79, ethanone B85, fenarimol B86, fenbuconazole B87, fenpropidine B88, fenpyrazamine B90, mefentrifluconazole B91, nuarimol B92, oxpoconazole B93, pefurazoate B94, prochloraz B95, spiroxamine B96, pyrisoxazole B97, simeconazole B104, ipfentrifluconazoleB106, edifenphos B111, iprobenfos B114, isoprothiolane B115, oxathiapiprolin B116, fluoxapiproline B117, propamocarb B118, prothiocarb B119, pyrazophos B120, quintozene tozene B121, tolclofos-methyl B122, benthiavalicarb-isopropyl B123, polyoxin B124, valifenalate B125, pyrimorph B126, cyprodinil B128, kasugamycin B129, mepani pyrim B130, pyrimethanil B131, streptomycin B132, zineb B134, mancozeb B135, metiram B136, propineb B137, maneb B138, amobam B139, milneb B142, mancopper B143 , sulfur (sulphur) B145, anilazine (anilazine) B146, captan (captan) B147, chlorothalonil (chlorothalonil) B149, dichlofluanid (dichlofluanid) B150, tolylfluanid (tolylfluanid) B151, methasulfocarb (methasulfocarb) B154, captafol (captafol) B155, folpet (folpet) B156, zinc thiazole (zinc thiazole B159, oxine-copper B160, thiodiazole-copper B161, cuppric nonyl phenolsulfonate B162, picarbutrazox B163, aminopyrifen B164, metyltetraprole B165, pyridachlometyl B167, validamycin B169, ferimzone B170, tebufloquinOne or more of B171, quinofumelin B173, ipflufenoquin B174, dipymetitrone B175, and teclofthalam B176.

[0053] Still further preferably, the composition comprises active component A and active component B in a weight ratio of 1:10-10:1;

[0054] Wherein, the active component A is selected from one or more of the compounds of formula IB or their salts;

[0055] wherein R1 is selected from hydrogen, halogen, cyano, nitro, amino, carboxyl or C1-C4 alkyl; R2 is selected from hydrogen, halogen, cyano, nitro, amino, carboxyl, formyl or C1-C4 alkyl; R3 is selected from hydrogen, hydroxyl, formyl or C1-C4 alkyl; R4 and R5 may be the same or different and are selected from hydrogen, halogen or C1-C4 alkyl; R6 and R7 may be the same or different and are selected from hydrogen, halogen or C1-C4 alkyl; R8 and R9 may be the same or different and are selected from hydrogen, cyano, halogen or C1-C4 alkyl; R 11 is selected from halogen, hydroxyl, amino, cyano, nitro or C1-C4 alkyl; n is selected from an integer from 0 to 5, when n is 0, there is no substituent on the benzene ring; when n is greater than 1, R 11 May be the same or different; W is selected from hydrogen, halogen or C1-C4 alkyl;

[0056] The salt of the compound represented by the general formula IB is a salt formed by the compound of the general formula with hydrochloric acid, sulfuric acid, phosphoric acid, formic acid or acetic acid;

[0057] The active ingredient B is selected from benzovindiflupyr B1, penflufen B2, isopyrazam B3, fluxapyroxad B4, fluopyram B5, flubeneteram B6, sedaxane B7, penthiopyrad B8, boscalid B9, bixafen B10, thifluzamide B13, pydiflumetofen B14, fluindapyr B15, fluopimomide B16, inpyrfluxam B18, isoflurane B19, pyraziflumid B20, pyrapropoyne B21, benodanil B26, fluopicolide B27, fenamidone B28, famoxadone B29, cyazofamid B30, ametoctradin B31, fluazinam B32, fenpicoxamid B33, florylpicoxamid B34, silthiopham B36, flumorph B41, dimethomorph B42, mandipropamid B43, zoxamide B44, metalaxyl-M B46, iprovalicarb B47, cymoxanil B48, tiadinil B51, ethaboxam B52, pyrimidine sulfonate Bupirimate B56, ethirimol B58, hymexazol B59, octhilinone B60, chinomethionat B62, fludioxonil B65, iprodione B67, procymidone B68, diethofencarb B73, metrafenone B74, pyriofenoneB75, carbendazim, B76, thiophanate-methyl, B77, phenamacril, B78, fenbuconazole, B87, mefentrifluconazole, B91, nuarimol, B92, prochloraz, B95, spiroxamine, B96, pyrisoxazole, B97, simeconazole, B104, ipfentrifluconazole B106, iprobenfos B114, isoprothiolane B115, oxathiapiprolin B116, fluoxapiproline B117, propamocarb B118, quintozene B121, tolclofos-methyl B122, benthiavalicarb-isopropyl B123, polyoxin B124, valifenalate B125, pyrimorph B126, cyprodinil B128, kasugamycin B129, pyrimorph Pyrimethanil B131, Streptomycin B132, Zineb B134, Mancozeb B135, Metiram B136, Propineb B137, Sulphur B145, Captan B147, Chlorothalonil B149, Dichlofluanid B150, Captafol B155, Folpet B156, Zinc Thiazole B159, Oxine-Copper B160, Thiodiazole-Copper B161, Cupric nonyl phenolsulfonate)B162, picarbutrazox B163, aminopyrifen B164, metyltetraprole B165, pyridachlometylOne or more of B167, validamycin B169, tebufloquin B171, quinofumelin B173, ipflufenoquin B174, dipymetitrone B175, and teclofthalam B176.

[0058] Application of a bactericidal composition for preparing a medicine for preventing and treating plant pathogenic fungi and bacterial diseases

[0059] The plant pathogenic fungi and bacterial diseases are plant powdery mildew, rust, black spot, leaf mold, sheath blight, damping-off, black mole, smut, powdery mildew, downy mildew, late blight, blight, downy mildew, black shank, cotton rot, damping-off, cotton blight, white rust, black spot, leaf spot, early blight, anthrax, brown spot, vine blight, gray mold, sclerotinia, rot, root rot, head mold, soft rot, white leaf blight, leaf blight, angular spot, round spot, white leaf blight, bacterial wilt, canker or yellow greening disease.

[0060] A bactericidal preparation, the active ingredient of the preparation is the bactericidal composition, and the weight percentage of the bactericidal composition is 0.1-95%.

[0061] Depending on the extent of crop diseases, the composition of the present invention is used at a concentration of 5-500 mg / L (active ingredient content, the same below), preferably 50-200 mg / L, in crop planting areas.

[0062] The active component A and at least one active component B in the composition of the present invention are prepared in advance according to the appropriate ratio provided by the present invention, or prepared on site, or the two components are used separately and sequentially.

[0063] The composition of the present invention is suitable for use in preventing and controlling fungal and bacterial diseases of trees (apple, rubber, pear, citrus, hawthorn, chestnut, pepper, wolfberry, mango, papaya, litchi, banana, peach, etc.), vines (grapes, etc.), melons and vegetables (tomato, eggplant, pepper, cucumber, melon, wax gourd, watermelon, pumpkin, bitter melon, loofah, chayote, gourd, zucchini, lettuce, potato, carrot), beans (pea, kidney bean, cowpea), cereals (wheat, rice, corn, sorghum, etc.), oil crops (rape, soybean, peanut, sesame, etc.), onions and garlic (scallion, garlic, onion), economic crops (tobacco, etc.), flowers, plants and lawns, as well as seed treatment, fruit preservation and other applications.

[0064] The fungicidal composition of the present invention is particularly suitable for preventing and controlling the following plant diseases: powdery mildew of apple trees, powdery mildew of rubber trees, powdery mildew of citrus, powdery mildew of hawthorn, powdery mildew of chestnut, powdery mildew of pepper, powdery mildew of wolfberry, powdery mildew of mango, powdery mildew of papaya, powdery mildew of corn, powdery mildew of grapes, powdery mildew of tomatoes, powdery mildew of eggplants, powdery mildew of peppers, powdery mildew of cucumbers, powdery mildew of melons, powdery mildew of wax gourds, powdery mildew of watermelons, powdery mildew of pumpkins, powdery mildew of bitter melons, powdery mildew of loofahs, powdery mildew of chayote, powdery mildew of cucurbits, powdery mildew of zucchini, powdery mildew of lettuce, and powdery mildew of peas. , powdery mildew of beans, powdery mildew of wheat, powdery mildew of barley, powdery mildew of roses, powdery mildew of roses, powdery mildew of chrysanthemums, powdery mildew of turf grass, powdery mildew of carrots, rust of apple trees, rust of pear trees, rust of soybeans, rust of peas, rust of beans, rust of cowpeas, rust of wheat, rust of barley, rust of corn, rust of onions, rust of garlic, rust of onions, rust of turf grass, anthracnose of apple trees, anthracnose of rubber trees, anthracnose of pear trees, anthracnose of citrus trees, anthracnose of hawthorn trees, anthracnose of chestnuts, anthracnose of peppers, anthracnose of wolfberries, anthracnose of mangoes, anthracnose of papayas, anthracnose of hazelnuts Anthracnose, grape anthracnose, tomato anthracnose, eggplant anthracnose, pepper anthracnose, melon anthracnose, winter melon anthracnose, watermelon anthracnose, pumpkin anthracnose, bitter melon anthracnose, loofah anthracnose, chayote anthracnose, gourd anthracnose, zucchini anthracnose, lettuce anthracnose, cucumber downy mildew, grape downy mildew, melon downy mildew, bitter melon downy mildew, loofah downy mildew, tomato late blight, potato late blight, pepper blight, cucumber damping-off, eggplant damping-off, pepper damping-off, pear brown spot, cucumber brown spot, pear black spot, rose black spot, rose Black spot disease, chrysanthemum black spot disease, soybean brown spot disease, cowpea red spot disease, apple leaf spot disease, bean ring spot disease, eggplant coryneform leaf spot disease, potato black mole disease, wheat eyelash blight, rice eyelash blight, rice seedling blight, soybean damping-off disease, pea damping-off disease, kidney bean damping-off disease, onion damping-off disease, garlic damping-off disease, onion damping-off disease, turfgrass damping-off disease, citrus canker, citrus Huanglongbing disease, rice white leaf blight, rice bacterial streak disease, tomato bacterial wilt disease, cucumber bacterial angular leaf spot disease, and crop gray mold and sclerotinia disease caused by pathogens of the genera Botrytis cinerea and Sclerotinia.

[0065] The present invention has the advantages:

[0066] The present invention combines active component A and fungicide active component B in varying proportions, resulting in a significant synergistic effect with observed efficacy exceeding calculated efficacy. This reduces the dosage of the pesticide and reduces environmental pollution. The present invention also uses a blend of fungicides with different mechanisms of action, helping to slow the development of drug resistance in pathogens. This provides an effective solution to the fungicide resistance problem faced by agricultural production and the pesticide industry, extending the lifespan of the pesticide. DETAILED DESCRIPTION

[0067] The synergistic effect of the composition of the present invention on harmful fungal and bacterial diseases can be further illustrated by the following examples, but the present invention is by no means limited thereto. The active ingredients described therein are compound A and fungicide B in the fungicidal composition of the present invention;

[0068] The preparation of compound A refers to the description in WO2016184378, and the fungicide B is selected from one or more of respiratory inhibitor fungicides, nucleic acid metabolism inhibitor fungicides, cell wall synthesis inhibitor fungicides, signal transduction inhibitor fungicides, cytoskeleton and motor protein inhibitor fungicides, sterol biosynthesis inhibitor fungicides, phospholipid synthesis inhibitor fungicides, cell wall synthesis inhibitor fungicides, methionine biosynthesis inhibitor fungicides, multi-site inhibitor fungicides, and fungicides with unknown mechanisms of action.

[0069] The test methods and evaluation methods are as follows:

[0070] The active samples to be tested are active component A, active component B, and a combination of active component A and active component B.

[0071] The active component A is one or more of active component A1, active component A2, active component A3 and active component A4;

[0072] Wherein, the active component A1 is compound A1 represented by the general formula IA;

[0073] Active component A2 is a salt formed by compound A1 and sulfuric acid;

[0074] Compound A1 is

[0075]

[0076] In the formula, R3 is selected from hydrogen, R4 and R5 are selected from hydrogen, R6 and R7 are selected from hydrogen, R8 and R9 are selected from hydrogen, R 11 Selected from 4-chloro, n=1, R 14 、R 15 、R 16 or R 17 are respectively selected from hydrogen, and W is selected from hydrogen, which is compound A1;

[0077] The active component A3 is compound A3 represented by the general formula IB;

[0078] Active ingredient A4 is a salt formed by compound A3 and sulfuric acid:

[0079] Compound A3 is

[0080]

[0081] Where:

[0082] R3 is selected from hydrogen, R4 and R5 are selected from hydrogen, R6 and R7 are selected from hydrogen, R8 and R9 are selected from hydrogen, R 11 Selected from 4-chloro, n=1, R 14 、R 15 、R 16 or R 17 are respectively selected from hydrogen, and W is selected from hydrogen, which is compound A3.

[0083] Active ingredient B is benzovinflupyr B1, fluopyram B4, fluopyram B5, boscalid B9, thiophanate B13, fluopyram B14, fluopyram B27, pyraclostrobin B31, fluazinam B32, fenpicoxamid B33, flumorph B41, dimethomorph B42, dimethomorph B43, zoxamid B44, metalaxyl B46, pyrimidine sulfonate B56, pyrimidine B58, fludioxonil B65, diethofencarb B73, mefenoxam B74, cyproconazole B78, clofosconazole B91, prochloraz B95, fluthiazolinone B116, fluoxapiproline B117, polyoxin B124, kasugamycin B129, mancozeb B134, mancozeb B135, methotrexate B136, propineb B137, captan B147, chlorothalonil B149, zinc thiazole B159, quinoline copper B160, picarbutrazox B163, aminopyrifen B164, metyltetraprole B165.

[0084] The composition to be tested is active component A1, each active component B, active component A1 and each active component B; wherein, active component B is benzovinflupyr B1, fluopyram B4, fluopyram B5, boscalid B9, thiophanate B13, fluopyram B14, fluopyram B27, pyraclostrobin B31, fluazinam B32, fenpicoxamid B33, flumorph B41, dimethomorph B42, dimethomorph B43, zoxamid B44, metalaxyl B46, pyrimidine sulfonate B56, pyrimidine B58, fludioxonil B65, diethofencarb B73, metrafenone B74, cyproconazole B78, clofosconazole B91, prochloraz B95, fluthiazolinone B116, fluoxapiproline A composition consisting of B117, polyoxin B124, kasugamycin B129, mancozeb B134, mancozeb B135, methotrexate B136, propineb B137, captan B147, chlorothalonil B149, zinc thiazole B159, quinoline copper B160, picarbutrazox B163, aminopyrifen B164, and metyltetraprole B165.

[0085] The composition to be tested is the active component A2 and / or ... A composition consisting of B117, polyoxin B124, kasugamycin B129, mancozeb B134, mancozeb B135, methotrexate B136, propineb B137, captan B147, chlorothalonil B149, zinc thiazole B159, quinoline copper B160, picarbutrazox B163, aminopyrifen B164, and metyltetraprole B165.

[0086] The composition to be tested is the active component A3 and / or benzovinflupyr B1, fluopyram B4, fluopyram B5, boscalid B9, thiophanate B13, fluopyram B14, fluopyram B27, pyraclostrobin B31, fluazinam B32, fenpicoxamid B33, flumorph B41, dimethomorph B42, dimethomorph B43, zoxamid B44, metalaxyl B46, pyrimidine sulfonate B56, pyrimidine B58, fludioxonil B65, diethofencarb B73, metrafenone B74, cyproconazole B78, clofosconazole B91, prochloraz B95, fluthiazolinone B116, fluoxapiproline A composition consisting of B117, polyoxin B124, kasugamycin B129, mancozeb B134, mancozeb B135, methotrexate B136, propineb B137, captan B147, chlorothalonil B149, zinc thiazole B159, quinoline copper B160, picarbutrazox B163, aminopyrifen B164, and metyltetraprole B165.

[0087] The composition to be tested is the active component A4 and / or ... A composition consisting of B117, polyoxin B124, kasugamycin B129, mancozeb B134, mancozeb B135, methotrexate B136, propineb B137, captan B147, chlorothalonil B149, zinc thiazole B159, quinoline copper B160, picarbutrazox B163, aminopyrifen B164, and metyltetraprole B165.

[0088] Specific method:

[0089] Dissolve each of the above active ingredients or compositions in acetone (the volume ratio of acetone to spray volume is equal to or less than 0.05), dilute with water containing 0.1% Tween 80, and prepare a test solution of the desired concentration. Separately, prepare a test solution of the composition according to the set ratio. Using a crop sprayer, spray the test solution onto uniformly arranged host plants cultured in a greenhouse (uninoculated and not treated with other drugs prior to application). Inoculate the disease 24 hours later. Depending on the characteristics of the disease, inoculate diseased plants requiring temperature-controlled, moisturizing cultivation in a climate chamber. After infection is complete, transfer to the greenhouse. Inoculate diseased plants not requiring moisturizing cultivation and directly transfer to the greenhouse. Once the control plants are fully diseased, conduct a disease survey and record the total number of leaves surveyed, the number of diseased leaves, and the disease severity.

[0090] Disease classification method:

[0091] Level 0: The whole plant is disease-free;

[0092] Level 1: The lesion area is less than 5% of the entire leaf area;

[0093] Level 3: The lesion area accounts for 6-10% of the entire leaf area;

[0094] Level 5: The lesion area accounts for 11-20% of the entire leaf area;

[0095] Level 7: The lesion area accounts for 21-50% of the entire leaf area;

[0096] Level 9: The lesion area accounts for more than 51% of the entire leaf area.

[0097] Observed efficacy of active ingredient or composition (C obs ) is calculated using the commonly used formula for pesticide efficacy evaluation (corrected control efficacy calculation formula):

[0098]

[0099] An efficacy of "0" means that the level of infection in the treated crop is the same as the level of infection in the untreated control crop; an efficacy of "100" means that the treated crop is not infected.

[0100] The expected efficacy of the composition (C exp ) was determined using the Abbott method (see Liu Xuemin et al., Synergistic Effect of Mixed Fungicides, Pesticide Science and Management, 2002, 23(5), 12-15).

[0101] C exp =X+Y–XY / 100

[0102] Where:

[0103] X: the efficacy of active ingredient A at concentration a;

[0104] Y: Efficacy of active ingredient B at concentration b.

[0105] The synergistic effect of the combination was observed by the efficacy (C obs ) and expected effectiveness (C exp When the ratio (synergistic ratio) is greater than 1, the composition exhibits a synergistic effect; when the ratio (synergistic ratio) = 1, the composition exhibits an additive effect; and when the ratio (synergistic ratio) is less than 1, the composition exhibits an antagonistic effect.

[0106] Example 1: Experiment 1 on preventing and controlling cucumber powdery mildew

[0107] Potted two-leaf cucumber seedlings of the variety "Jinyan-4" were sprayed with aqueous solutions of the active ingredients or compositions (active ingredient concentrations as described in the table below). 24 hours later, spores of yellow powdery mildew were sprayed onto the leaves using a dispenser. The leaves were cultured in a greenhouse and disease investigations were conducted 10 days later after the control group had fully developed disease.

[0108] The activity data and synergistic effects of each individual active ingredient and the composition of the present invention in controlling cucumber powdery mildew are shown in Tables 1 and 2.

[0109] The results in Table 2 below show that the observed efficacy of the composition (C obs ) and expected effectiveness (C exp ) ratios are all >1, indicating that the composition exhibits a synergistic effect on cucumber powdery mildew within the test ratio range.

[0110] Table 1 Activity of individual active ingredients

[0111]

[0112] Table 2 Activity and synergistic effect of the composition of the present invention

[0113]

[0114]

[0115] Example 2: Test 2 for preventing and controlling cucumber powdery mildew

[0116] Potted two-leaf cucumber seedlings of the variety "Xintai Mici" were sprayed with aqueous solutions of the active ingredients or compositions (active ingredient concentrations are described in the table below). 24 hours later, spores of yellow powdery mildew were sprayed onto the leaves using a dispenser. The leaves were cultured in a greenhouse and disease investigations were conducted 10 days later after the control group had fully developed disease.

[0117] The activity data and synergistic effects of each individual active ingredient and the composition of the present invention in controlling cucumber powdery mildew are shown in Tables 3 and 4.

[0118] The results in Table 4 below show that the observed efficacy of the composition (C obs) and expected effectiveness (C exp ) ratios are all >1, indicating that the composition exhibits a synergistic effect on cucumber powdery mildew within the test ratio range.

[0119] Table 3 Activity of individual active ingredients

[0120]

[0121]

[0122] Table 4 Activity and synergistic effect of the composition of the present invention

[0123]

[0124]

[0125] Example 3: Test on Controlling Powdery Mildew of Muskmelon

[0126] Potted four-leaf muskmelon seedlings of the "Mixian Melon" variety (1-2 leaves were removed before the test, leaving only 3-4 leaves) were sprayed with aqueous solutions of the active ingredients or compositions (at the concentrations described in the table below). 24 hours later, a spore suspension of powdery mildew fungus was inoculated on the leaves and cultured in a greenhouse. Fifteen days later, after the control group had fully developed disease, a disease investigation was conducted.

[0127] The activity data of each individual active ingredient of the present invention in controlling melon powdery mildew are shown in Table 5.

[0128] The activity data and synergistic effect of the composition of the present invention in controlling melon powdery mildew are shown in Table 6.

[0129] The results in Table 6 below show that the observed efficacy of the composition (C obs ) and expected effectiveness (C exp ) ratios are all >1, indicating that the composition has a synergistic effect on melon powdery mildew within the test ratio range.

[0130] Table 5 Activity of individual active ingredients

[0131]

[0132]

[0133] Table 6 Activity and synergistic effect of the composition of the present invention

[0134]

[0135] Example 4 Test on Controlling Powdery Mildew of Pepper

[0136] Potted six-leaf pepper seedlings of the "Green Bell Pepper" variety were sprayed with aqueous solutions of the active ingredients or compositions (active ingredient concentrations are described in the table below). 24 hours later, spores of pepper powdery mildew were inoculated on the undersides of the leaves and cultured in a climate chamber. After the disease infection was complete, the leaves were moved to a greenhouse for culture. After 20-25 days, the disease was investigated after the controls had fully developed the disease.

[0137] The activity data of each individual active ingredient of the present invention in controlling powdery mildew of pepper are shown in Tables 7 and 8.

[0138] The activity data and synergistic effect of the composition of the present invention in controlling powdery mildew of pepper are shown in Tables 9 and 10.

[0139] The results in Tables 9 and 10 below show that the observed efficacy of the composition (C obs ) and expected effectiveness (C exp ) ratios are all >1, indicating that the composition has a synergistic effect on pepper powdery mildew within the test ratio range.

[0140] Table 7 Activity of individual active ingredients

[0141]

[0142] Table 8 Activity of individual active ingredients

[0143]

[0144] Table 9 Activity and synergistic effect of the composition of the present invention

[0145]

[0146] Table 10 Activity and synergistic effect of the composition of the present invention

[0147]

[0148] Example 5 Test on Control of Kidney Bean Powdery Mildew

[0149] Potted two-leaf bean seedlings of the variety "Fengshou No. 1" were sprayed with aqueous solutions of the active ingredients or compositions (the concentrations of the active ingredients are as shown in the table below). After spraying, they were naturally dried. After 24 hours, an aqueous suspension of spores of the bean powdery mildew pathogen (5×10 6 The bacteria were inoculated on bean leaves with 100 μg / ml, dried naturally, and then moved into the greenhouse for cultivation under the following culture conditions (temperature: 23-28°C during the day and 18-20°C at night). The disease was investigated after the control group became fully diseased.

[0150] The activity data of each individual active ingredient of the present invention in controlling bean powdery mildew are shown in Table 11.

[0151] The activity data and synergistic effect of the composition of the present invention in controlling bean powdery mildew are shown in Table 12.

[0152] The results in Table 12 below show that the observed efficacy of the composition (C obs ) and expected effectiveness (C exp ) ratios are all >1, indicating that the composition has a synergistic effect on bean powdery mildew within the test ratio range.

[0153] Table 11 Activity of individual active ingredients

[0154]

[0155] Table 12 Activity and synergistic effect of the composition of the present invention

[0156]

[0157] Example 6 Test on Control of Tobacco Powdery Mildew

[0158] Potted tobacco seedlings of the variety "CN89" at the six-leaf stage were sprayed with aqueous solutions of the active ingredients or compositions (at the concentrations described in the table below). 24 hours later, a spore suspension of tobacco powdery mildew was inoculated onto the leaves of the tobacco seedlings. The seedlings were directly transferred to a greenhouse for cultivation. After 20 days, the disease was investigated after the control group had fully developed the disease.

[0159] The activity data of each individual active ingredient of the present invention in controlling tobacco powdery mildew are shown in Table 13.

[0160] The activity data and synergistic effect of the composition of the present invention in controlling tobacco powdery mildew are shown in Table 14.

[0161] The results in Table 14 below show that the observed efficacy of the composition (C obs ) and expected effectiveness (C exp ) ratios are all >1, indicating that the composition exhibits a synergistic effect on tobacco powdery mildew within the test ratio range.

[0162] Table 13 Activity of individual active ingredients

[0163]

[0164]

[0165] Table 14 Activity and synergistic effect of the composition of the present invention

[0166]

[0167] Example 7 Corn rust control test

[0168] Potted two-leaf-stage corn seedlings of the "Golden Glutinous" variety were sprayed with an aqueous solution of each active ingredient or composition (active ingredient concentrations are described in the table below). 24 hours later, a spore suspension of corn rust was inoculated on the corn leaves and cultured in a climate chamber. After the disease infection was complete, the leaves were moved to a greenhouse for culture. After 7 days, the disease was investigated after the control had fully developed the disease.

[0169] The activity data of each individual active ingredient of the present invention for controlling corn rust are shown in Table 15.

[0170] The activity data and synergistic effect of the composition of the present invention in controlling corn rust are shown in Table 16.

[0171] The results in Table 16 below show that the observed efficacy of the composition (C obs ) and expected effectiveness (C exp ) ratios are all >1, indicating that the composition has a synergistic effect on corn rust within the test ratio range.

[0172] Table 15 Activity of individual active ingredients

[0173]

[0174] Table 16 Activity and synergistic effect of the composition of the present invention

[0175]

[0176]

[0177] Example 8: Test 1 for preventing and controlling cucumber downy mildew

[0178] Potted two-leaf stage cucumber seedlings of the variety "Xintai Mici" were sprayed with an aqueous solution of each active ingredient or composition (the concentration of the active ingredient is described in the table below). After 24 hours, a sporangium suspension of the cucumber downy mildew pathogen was inoculated on the cucumber leaves and cultured in a climate chamber. After the disease infection was complete, the leaves were moved to a greenhouse for culture. After 7 days, the disease was investigated after the control group was fully diseased.

[0179] The activity data of each individual active ingredient of the present invention for controlling cucumber downy mildew are shown in Table 17.

[0180] The activity data and synergistic effect of the composition of the present invention in controlling cucumber downy mildew are shown in Table 18.

[0181] The results in Table 18 below show that the ratios of the observed efficacy (Cobs) and expected efficacy (Cexp) of the composition are both greater than 1, indicating that the composition exhibits a synergistic effect on cucumber downy mildew within the test ratio range.

[0182] Table 17 Activity of individual active ingredients

[0183]

[0184]

[0185] Table 18 Activity and synergistic effect of the composition of the present invention

[0186]

[0187]

[0188]

[0189] Example 9: Test 2 for preventing and controlling cucumber downy mildew

[0190] Potted two-leaf stage cucumber seedlings of the variety "Xintai Mici" were sprayed with an aqueous solution of each active ingredient or composition (the concentration of the active ingredient is described in the table below). After 24 hours, a sporangium suspension of the cucumber downy mildew pathogen was inoculated on the cucumber leaves and cultured in a climate chamber. After the disease infection was complete, the leaves were moved to a greenhouse for culture. After 7 days, the disease was investigated after the control group was fully diseased.

[0191] The activity data of each individual active ingredient of the present invention for controlling cucumber downy mildew are shown in Table 19.

[0192] The activity data and synergistic effect of the composition of the present invention in controlling cucumber downy mildew are shown in Table 20.

[0193] The results in Table 20 below show that the ratios of the observed efficacy (Cobs) and expected efficacy (Cexp) of the composition are both greater than 1, indicating that the composition exhibits a synergistic effect on cucumber downy mildew within the test ratio range.

[0194] Table 19 Activity of individual active ingredients

[0195]

[0196] Table 20 Activity and synergistic effect of the composition of the present invention

[0197]

[0198]

[0199]

[0200] Example 10: Experiment 3 on the prevention and treatment of cucumber downy mildew

[0201] The test was conducted in accordance with the national standard GB / T 17980.26-2000: Guidelines for Field Efficacy Tests of Pesticides (I) - Control of Cucumber Downy Mildew with Fungicides. The test site was located in a protected area in Bayi Town, Sujiatun District, Shenyang City. The cucumber variety was Xintai Michai, with a moderate level of management. At the time of the test, the cucumbers were in the fruiting stage and had a mild incidence of downy mildew. The plot area was approximately 25 m2. 2 Cucumbers were sprayed with the pesticide at the designated dosage. The pesticide was applied twice, with a 7-day interval between applications. Ten days after the second application, the incidence of downy mildew in cucumbers was investigated in each plot. Samples were collected from four locations in each plot, with eight plants sampled at each location. The disease was examined from top to bottom on the entire leaf of each plant.

[0202] The activity data of each individual active ingredient of the present invention for controlling cucumber downy mildew are shown in Table 21.

[0203] The activity data and synergistic effect of the composition of the present invention in controlling cucumber downy mildew are shown in Table 22.

[0204] The results in Table 22 below show that the ratios of the observed efficacy (Cobs) and expected efficacy (Cexp) of the composition are both greater than 1, indicating that the composition exhibits a synergistic effect on cucumber downy mildew within the test ratio range.

[0205] Table 21 Activity of individual active ingredients

[0206]

[0207] Table 22 Activity and synergistic effect of the composition of the present invention

[0208]

[0209] Example 11: Test 4 for preventing and controlling cucumber downy mildew

[0210] Potted two-leaf stage cucumber seedlings of the variety "Xintai Mici" were sprayed with an aqueous solution of each active ingredient or composition (the concentration of the active ingredient is described in the table below). After 24 hours, a sporangium suspension of the cucumber downy mildew pathogen was inoculated on the cucumber leaves and cultured in a climate chamber. After the disease infection was complete, the leaves were moved to a greenhouse for culture. After 7 days, the disease was investigated after the control group was fully diseased.

[0211] The activity data of each individual active ingredient of the present invention in controlling cucumber downy mildew are shown in Table 23.

[0212] The activity data and synergistic effect of the composition of the present invention in controlling cucumber downy mildew are shown in Table 24.

[0213] The results in Table 24 below show that the ratios of the observed efficacy (Cobs) and expected efficacy (Cexp) of the composition are both greater than 1, indicating that the composition exhibits a synergistic effect on cucumber downy mildew within the test ratio range.

[0214] Table 23 Activity of individual active ingredients

[0215]

[0216] Table 24 Activity and synergistic effect of the composition of the present invention

[0217]

[0218]

[0219] Example 12: Experiment 1 on the prevention and treatment of grape downy mildew

[0220] Potted five-leaf grape seedlings of the "Seedless White Chicken Heart" variety were sprayed with aqueous solutions of the active ingredients or compositions (active ingredient concentrations are described in the table below). 24 hours later, a sporangium suspension of grape downy mildew pathogen was inoculated onto the grape leaves. The grape seedlings were then cultured in a climate chamber. After infection was complete, they were moved to a greenhouse for culture. Seven days later, after the control group had fully developed the disease, a disease investigation was conducted.

[0221] The activity data of each individual active ingredient of the present invention in controlling grape downy mildew are shown in Table 25.

[0222] The activity data and synergistic effect of the composition of the present invention in controlling grape downy mildew are shown in Table 26.

[0223] The results in Table 26 below show that the ratios of the observed efficacy (Cobs) and expected efficacy (Cexp) of the composition are both greater than 1, indicating that the composition exhibits a synergistic effect on grape downy mildew within the test ratio range.

[0224] Table 25 Activity of individual active ingredients

[0225]

[0226]

[0227] Table 26 Activity and synergistic effect of the composition of the present invention

[0228]

[0229]

[0230] Example 13: Test 2 for controlling grape downy mildew

[0231] Potted five-leaf grape seedlings of the "Seedless White Chicken Heart" variety were sprayed with aqueous solutions of the active ingredients or compositions (active ingredient concentrations are described in the table below). 24 hours later, a sporangium suspension of grape downy mildew pathogen was inoculated onto the grape leaves. The grape seedlings were then cultured in a climate chamber. After infection was complete, they were moved to a greenhouse for culture. Seven days later, after the control group had fully developed the disease, a disease investigation was conducted.

[0232] The activity data of each individual active ingredient of the present invention in controlling grape downy mildew are shown in Table 27.

[0233] The activity data and synergistic effect of the composition of the present invention in controlling grape downy mildew are shown in Table 28.

[0234] The results in Table 28 below show that the ratios of the observed efficacy (Cobs) and expected efficacy (Cexp) of the composition are both greater than 1, indicating that the composition exhibits a synergistic effect on grape downy mildew within the test ratio range.

[0235] Table 27 Activity of individual active ingredients

[0236]

[0237] Table 28 Activity and synergistic effect of the composition of the present invention

[0238]

[0239]

[0240] Example 14: Experiment 3 on the prevention and treatment of grape downy mildew

[0241] Potted five-leaf grape seedlings of the "Seedless White Chicken Heart" variety were sprayed with aqueous solutions of the active ingredients or compositions (active ingredient concentrations are described in the table below). 24 hours later, a sporangium suspension of grape downy mildew pathogen was inoculated onto the grape leaves. The grape seedlings were then cultured in a climate chamber. After infection was complete, they were moved to a greenhouse for culture. Seven days later, after the control group had fully developed the disease, a disease investigation was conducted.

[0242] The activity data of each individual active ingredient of the present invention for controlling grape downy mildew are shown in Table 29.

[0243] The activity data and synergistic effect of the composition of the present invention in controlling grape downy mildew are shown in Table 30.

[0244] The results in Table 30 below show that the ratios of the observed efficacy (Cobs) and expected efficacy (Cexp) of the composition are both greater than 1, indicating that the composition exhibits a synergistic effect on grape downy mildew within the test ratio range.

[0245] Table 29 Activity of individual active ingredients

[0246]

[0247] Table 30 Activity and synergistic effect of the composition of the present invention

[0248]

[0249]

[0250] Example 15: Test 4 for controlling grape downy mildew

[0251] Potted five-leaf grape seedlings of the "Seedless White Chicken Heart" variety were sprayed with aqueous solutions of the active ingredients or compositions (active ingredient concentrations are described in the table below). 24 hours later, a sporangium suspension of grape downy mildew pathogen was inoculated onto the grape leaves. The grape seedlings were then cultured in a climate chamber. After infection was complete, they were moved to a greenhouse for culture. Seven days later, after the control group had fully developed the disease, a disease investigation was conducted.

[0252] The activity data of each individual active ingredient of the present invention in controlling grape downy mildew are shown in Table 31.

[0253] The activity data and synergistic effect of the composition of the present invention in controlling grape downy mildew are shown in Table 32.

[0254] The results in Table 32 below show that the ratios of the observed efficacy (Cobs) and expected efficacy (Cexp) of the composition are both greater than 1, indicating that the composition exhibits a synergistic effect on grape downy mildew within the test ratio range.

[0255] Table 31 Activity of individual active ingredients

[0256]

[0257]

[0258] Table 32 Activity and synergistic effect of the composition of the present invention

[0259]

[0260]

[0261] Example 16: Test on Control of Downy Mildew of Cabbage

[0262] Potted five-leaf cabbage seedlings of the "Si Ji Xiao Bai Cai" variety were sprayed with aqueous solutions of the active ingredients or compositions (active ingredient concentrations as described in the table below). 24 hours later, sporangia of P. cabbage downy mildew were inoculated on the undersides of the leaves. The leaves were cultured in a climate chamber. After the disease infection was complete, the leaves were moved to a greenhouse for culture. After 5-7 days, the disease was investigated after the control plants had fully developed disease.

[0263] The activity data of each individual active ingredient of the present invention for controlling downy mildew of cabbage are shown in Table 33.

[0264] The activity data and synergistic effect of the composition of the present invention in controlling cabbage downy mildew are shown in Table 34.

[0265] The results in Table 34 below show that the observed efficacy of the composition (C obs ) and expected effectiveness (C exp ) ratios are all >1, indicating that the composition has a synergistic effect on cabbage downy mildew within the test ratio range.

[0266] Table 33 Activity of individual active ingredients

[0267]

[0268]

[0269] Table 34 Activity and synergistic effect of the composition of the present invention

[0270]

[0271] Example 17 Test on Control of Sunflower Downy Mildew

[0272] Potted six-leaf sunflower seedlings of the "Sandaomei" variety (1-4 leaves were trimmed before the test, leaving only 5-6 leaves) were sprayed with aqueous solutions of the active ingredients or compositions (active ingredient concentrations are described in the table below). 24 hours later, a sporangium suspension of sunflower downy mildew pathogen was inoculated on the leaves and cultured in a climate chamber. After the disease infection was complete, the seedlings were moved to a greenhouse for culture. Twelve days later, after the control group had fully developed the disease, a disease investigation was conducted.

[0273] The activity data of each individual active ingredient of the present invention for controlling sunflower downy mildew are shown in Table 35.

[0274] The activity data and synergistic effect of the composition of the present invention in controlling sunflower downy mildew are shown in Table 36.

[0275] The results in Table 36 below show that the observed efficacy of the composition (C obs ) and expected effectiveness (C exp ) ratios are all >1, indicating that the composition exhibits a synergistic effect on sunflower downy mildew within the test ratio range.

[0276] Table 35 Activity of individual active ingredients

[0277]

[0278] Table 36 Activity and synergistic effect of the composition of the present invention

[0279]

[0280]

[0281] Example 18: Test 1 for controlling tomato late blight

[0282] Potted five-leaf tomato seedlings of the variety "Hontaro" were sprayed with aqueous solutions of the active ingredients or compositions (active ingredient concentrations as described in the table below). 24 hours later, a sporangium suspension of the tomato late blight pathogen was inoculated on the tomato leaves. The tomato seedlings were then cultured in a climate chamber. After the disease infection was complete, they were moved to a greenhouse for culture. After 7 days, the disease was investigated after the controls had fully developed disease.

[0283] The activity data of each individual active ingredient of the present invention in controlling tomato late blight are shown in Table 37.

[0284] The activity data and synergistic effect of the composition of the present invention in controlling tomato late blight are shown in Table 38.

[0285] The results in Table 38 below show that the ratios of the observed efficacy (Cobs) and expected efficacy (Cexp) of the composition are both greater than 1, indicating that the composition exhibits a synergistic effect on tomato late blight within the test ratio range.

[0286] Table 37 Activity of individual active ingredients

[0287]

[0288] Table 38 Activity and synergistic effect of the composition of the present invention

[0289]

[0290] Example 19: Test 2 for controlling tomato late blight

[0291] Potted five-leaf tomato seedlings of the variety "Hontaro" were sprayed with aqueous solutions of the active ingredients or compositions (active ingredient concentrations as described in the table below). 24 hours later, a sporangium suspension of the tomato late blight pathogen was inoculated on the tomato leaves. The tomato seedlings were then cultured in a climate chamber. After the disease infection was complete, they were moved to a greenhouse for culture. After 7 days, the disease was investigated after the controls had fully developed disease.

[0292] The activity data of each individual active ingredient of the present invention in controlling tomato late blight are shown in Table 39.

[0293] The activity data and synergistic effect of the composition of the present invention in controlling tomato late blight are shown in Table 40.

[0294] The results in Table 40 below show that the ratios of the observed efficacy (Cobs) and expected efficacy (Cexp) of the composition are both greater than 1, indicating that the composition exhibits a synergistic effect on tomato late blight within the test ratio range.

[0295] Table 39 Activity of individual active ingredients

[0296]

[0297] Table 40 Activity and synergistic effect of the composition of the present invention

[0298]

[0299]

[0300] Example 20: Experiment 1 on the prevention and treatment of cucumber anthracnose

[0301] Potted two-leaf stage cucumber seedlings of the variety "Xintai Mici" were sprayed with aqueous solutions of the active ingredients or compositions, and allowed to dry naturally after spraying. After 24 hours, an aqueous suspension of spores of the cucumber anthracnose pathogen was inoculated on the cucumber leaves and cultured in a climate chamber. After the disease infection was complete, the leaves were moved to a greenhouse for culture. After 7 days, the disease was investigated, and the leaves were graded according to the degree of development of the pathogen infection on the leaves. The total number of leaves investigated, the number of diseased leaves and the disease level were recorded, and the efficacy of the agent was calculated according to the formula.

[0302] The activity data of each individual active ingredient of the present invention in controlling cucumber anthracnose are shown in Table 41.

[0303] The activity data and synergistic effect of the composition of the present invention in controlling cucumber anthracnose are shown in Table 42.

[0304] The results in Table 42 below show that the ratios of the observed efficacy (Cobs) and expected efficacy (Cexp) of the composition are both greater than 1, indicating that the composition exhibits a synergistic effect on cucumber anthracnose within the test ratio range.

[0305] Table 41 Activity of individual active ingredients

[0306]

[0307] Table 42 Activity and synergistic effect of the composition of the present invention

[0308]

[0309] Example 21: Experiment 2 on the prevention and treatment of cucumber anthracnose

[0310] Potted two-leaf stage cucumber seedlings of the variety "Xintai Mici" were sprayed with aqueous solutions of the active ingredients or compositions, and allowed to dry naturally after spraying. After 24 hours, an aqueous suspension of spores of the cucumber anthracnose pathogen was inoculated on the cucumber leaves and cultured in a climate chamber. After the disease infection was complete, the leaves were moved to a greenhouse for culture. After 7 days, the disease was investigated, and the leaves were graded according to the degree of development of the pathogen infection on the leaves. The total number of leaves investigated, the number of diseased leaves and the disease level were recorded, and the efficacy of the agent was calculated according to the formula.

[0311] The activity data of each individual active ingredient of the present invention in controlling cucumber anthracnose are shown in Table 43.

[0312] The activity data and synergistic effect of the composition of the present invention in controlling cucumber anthracnose are shown in Table 44.

[0313] The results in Table 44 below show that the ratios of the observed efficacy (Cobs) and expected efficacy (Cexp) of the composition are both greater than 1, indicating that the composition exhibits a synergistic effect against cucumber anthracnose within the test ratio range.

[0314] Table 43 Activity of individual active ingredients

[0315]

[0316] Table 44 Activity and synergistic effect of the composition of the present invention

[0317]

[0318] Example 22 Experiment on the prevention and treatment of pepper anthracnose

[0319] Potted six-leaf pepper seedlings of the "Green Bell Pepper" variety were sprayed with an aqueous solution of each active ingredient or composition (active ingredient concentrations are described in the table below). 24 hours later, a spore suspension of pepper anthracnose fungus was inoculated on the underside of the leaves and cultured in a climate chamber. After the disease infection was complete, the leaves were moved to a greenhouse for culture. After 5-7 days, the disease was investigated after the control group had fully developed the disease.

[0320] The activity data of each individual active ingredient of the present invention in controlling pepper anthracnose are shown in Table 45.

[0321] The activity data and synergistic effect of the composition of the present invention in controlling pepper anthracnose are shown in Table 46.

[0322] The results in Table 46 below show that the observed efficacy of the composition (C obs ) and expected effectiveness (C exp ) ratios are all >1, indicating that the composition exhibits a synergistic effect on pepper anthracnose within the test ratio range.

[0323] Table 45 Activity of individual active ingredients

[0324]

[0325] Table 46 Activity and synergistic effect of the composition of the present invention

[0326]

[0327]

[0328] Example 23: Test on Controlling Eggplant Brown Spot Disease

[0329] Potted eggplant seedlings of the variety "Liaoqie No. 1" at the five-leaf stage were sprayed with aqueous solutions of the active ingredients or compositions (at the concentrations described in the table below). 24 hours later, a spore suspension of the eggplant brown spot pathogen was inoculated on the eggplant leaves and cultured in a climate chamber. After the disease infection was complete, the seedlings were moved to a greenhouse for culture. Ten days later, after the control group had fully developed the disease, a disease investigation was conducted.

[0330] The activity data of each individual active ingredient of the present invention in controlling eggplant brown spot disease are shown in Table 47.

[0331] The activity data and synergistic effect of the composition of the present invention in preventing and controlling eggplant brown spot disease are shown in Table 48.

[0332] The results in Table 48 below show that the observed efficacy of the composition (C obs ) and expected effectiveness (C exp ) ratios are all >1, indicating that the composition has a synergistic effect on eggplant brown spot disease within the test ratio range.

[0333] Table 47 Activity of individual active ingredients

[0334]

[0335]

[0336] Table 48 Activity and synergistic effect of the composition of the present invention

[0337]

[0338]

[0339] Example 24 Test on Controlling Eggplant Corynespora Leaf Spot

[0340] Potted eggplant seedlings of the variety "Liaoqie No. 1" at the five-leaf stage were sprayed with aqueous solutions of the active ingredients or compositions (at the concentrations described in the table below). 24 hours later, a spore suspension of the eggplant leaf spot pathogen was inoculated on the eggplant leaves and cultured in a climate chamber. After the disease infection was complete, the seedlings were moved to a greenhouse for culture. After 8 days, the disease was investigated after the control group had fully developed the disease.

[0341] The activity data of each individual active ingredient of the present invention in controlling eggplant corynespora leaf spot are shown in Table 49.

[0342] The activity data and synergistic effect of the composition of the present invention in controlling eggplant corynespora leaf spot are shown in Table 50.

[0343] The results in Table 50 below show that the observed efficacy of the composition (C obs ) and expected effectiveness (C exp) ratios are all >1, indicating that the composition has a synergistic effect on eggplant Corynespora leaf spot within the test ratio range.

[0344] Table 49 Activity of individual active ingredients

[0345]

[0346] Table 50 Activity and synergistic effect of the composition of the present invention

[0347]

[0348]

[0349]

[0350] Example 25: Test on Control of Pear Black Spot

[0351] Young leaves from potted "Xiangli" seedlings were surface disinfected, rinsed with sterile water, and dried in the shade for later use. The leaves were then immersed in aqueous solutions of the active ingredients or compositions (at the concentrations listed in the table below). Twenty-four hours later, a spore suspension of the pathogenic fungus Black Spot was inoculated onto the leaves. The leaves were then incubated in a Petri dish (containing 2% water agar) at a constant temperature and under light. After 14 days, the leaves were observed for signs of disease, once the control group had fully developed disease.

[0352] The activity data of each individual active ingredient of the present invention in controlling pear black spot are shown in Table 51.

[0353] The activity data and synergistic effect of the composition of the present invention in preventing and controlling pear black spot are shown in Table 52.

[0354] The results in Table 52 below show that the observed efficacy of the composition (C obs ) and expected effectiveness (C exp ) ratios are all >1, indicating that the composition has a synergistic effect on pear black spot within the test ratio range.

[0355] Table 51 Activity of individual active ingredients

[0356]

[0357]

[0358] Table 52 Activity and synergistic effect of the composition of the present invention

[0359]

[0360] Example 26 Peanut Leaf Spot Control Test 1

[0361] Two pairs of potted peanut seedlings of the variety "Baisha" in the flat-leaf stage were sprayed with an aqueous solution of each active component or composition (concentrations as described in the table below). 24 hours later, a spore suspension of peanut brown pathogen was inoculated on the leaves and cultured in a climate chamber. After the disease infection was complete, the leaves were moved to a greenhouse for culture. After 10-12 days, the disease was investigated after the control group was fully diseased.

[0362] The activity data of each individual active ingredient of the present invention in controlling peanut leaf spot disease are shown in Table 53.

[0363] The activity data and synergistic effect of the composition of the present invention in controlling peanut leaf spot are shown in Table 54.

[0364] The results in Table 54 below show that the observed efficacy of the composition (C obs ) and expected effectiveness (C exp ) ratios are all >1, indicating that the composition has a synergistic effect on peanut leaf spot within the test ratio range.

[0365] Table 53 Activity of individual active ingredients

[0366]

[0367]

[0368] Table 54 Activity and synergistic effect of the composition of the present invention

[0369]

[0370] Example 27 Peanut Leaf Spot Control Test 2

[0371] Two pairs of potted peanut seedlings of the variety "Baisha" in the flat-leaf stage were sprayed with an aqueous solution of each active component or composition (concentrations as described in the table below). 24 hours later, a spore suspension of peanut brown pathogen was inoculated on the leaves and cultured in a climate chamber. After the disease infection was complete, the leaves were moved to a greenhouse for culture. After 10-12 days, the disease was investigated after the control group was fully diseased.

[0372] The activity data of each individual active ingredient of the present invention in controlling peanut leaf spot disease are shown in Table 55.

[0373] The activity data and synergistic effect of the composition of the present invention in controlling peanut leaf spot are shown in Table 56.

[0374] The results in Table 56 below show that the observed efficacy of the composition (C obs ) and expected effectiveness (C exp ) ratios are all >1, indicating that the composition has a synergistic effect on peanut leaf spot within the test ratio range.

[0375] Table 55 Activity of individual active ingredients

[0376]

[0377] Table 56 Activity and synergistic effect of the composition of the present invention

[0378]

[0379] Example 28: Test for Controlling Wheat Scab

[0380] Potted wheat seedlings of the variety "Liaochun 18" at the two-leaf stage were sprayed with aqueous solutions of the active ingredients or compositions (at the concentrations described in the table below). 24 hours later, a spore suspension of Gibberella fusca was inoculated on the leaves and cultured in a greenhouse. Five days later, after the control group had fully developed the disease, a disease investigation was conducted.

[0381] The activity data of each individual active ingredient of the present invention in controlling wheat scab are shown in Table 57.

[0382] The activity data and synergistic effect of the composition of the present invention in controlling wheat scab are shown in Table 58.

[0383] The results in Table 58 below show that the observed efficacy of the composition (C obs ) and expected effectiveness (C exp ) ratios are all >1, indicating that the composition has a synergistic effect on wheat fusarium scab within the test ratio range.

[0384] Table 57 Activity of individual active ingredients

[0385]

[0386] Table 58 Activity and synergistic effect of the composition of the present invention

[0387]

[0388]

[0389] Example 29: Test 1 on Controlling Rice Sheath Blight

[0390] Potted two-leaf stage rice seedlings of the variety "Longdao 18" were sprayed with aqueous solutions of the active ingredients or compositions (at the concentrations described in the table below). 24 hours later, a mycelial suspension of Rhizoctonia solani was inoculated onto the rice leaves and cultured in a greenhouse. Five days later, disease activity was investigated after the controls had fully developed disease.

[0391] The activity data of each individual active ingredient of the present invention in controlling rice sheath blight are shown in Table 59.

[0392] The activity data and synergistic effect of the composition of the present invention in controlling rice sheath blight are shown in Table 60.

[0393] The results in Table 60 below show that the observed efficacy of the composition (C obs ) and expected effectiveness (C exp ) ratios are all >1, indicating that the composition exhibits a synergistic effect on rice sheath blight within the test ratio range.

[0394] Table 59 Activity of individual active ingredients

[0395]

[0396] Table 60 Activity and synergistic effect of the composition of the present invention

[0397]

[0398]

[0399] Example 30: Control of Rice Sheath Blight Test 2

[0400] Potted two-leaf stage rice seedlings of the variety "Longdao 18" were sprayed with aqueous solutions of the active ingredients or compositions (at the concentrations described in the table below). 24 hours later, a mycelial suspension of Rhizoctonia solani was inoculated onto the rice leaves and cultured in a greenhouse. Five days later, disease activity was investigated after the controls had fully developed disease.

[0401] The activity data of each individual active ingredient of the present invention in controlling rice sheath blight are shown in Table 61.

[0402] The activity data and synergistic effect of the composition of the present invention in controlling rice sheath blight are shown in Table 62.

[0403] The results in Table 62 below show that the observed efficacy of the composition (C obs ) and expected effectiveness (C exp ) ratios are all >1, indicating that the composition exhibits a synergistic effect on rice sheath blight within the test ratio range.

[0404] Table 61 Activity of individual active ingredients

[0405]

[0406]

[0407] Table 62 Activity and synergistic effect of the composition of the present invention

[0408]

[0409] Example 31 Test on Controlling Rice Bacterial Blight

[0410] Rice (Koshihikari) was planted in identical pots in a greenhouse, 8 plants per pot. When grown to the four-leaf stage, the plants were sprayed with aqueous solutions of the active ingredients or compositions. Twenty-four hours later, the activated bacterial blight pathogen was evenly sprayed on the rice leaves. The rice leaves were then moisturized in artificial air for 24 hours before being transferred to the greenhouse for further cultivation. After the clear water control had fully developed disease, the length of lesions on the leaves of each treatment was measured, and the disease inhibition rate (i.e., observation efficacy) was calculated using the following formula.

[0411]

[0412] The activity data of each individual active ingredient of the present invention in controlling rice bacterial blight are shown in Table 63.

[0413] The activity data and synergistic effect of the composition of the present invention in controlling rice bacterial blight are shown in Table 64.

[0414] The results in Table 64 below show that the observed efficacy of the composition (C obs ) and expected effectiveness (C exp ) ratios are all >1, indicating that the composition exhibits a synergistic effect on rice bacterial blight within the test ratio range.

[0415] Table 63 Activity of individual active ingredients

[0416]

[0417] Table 64 Activity and synergistic effect of the composition of the present invention

[0418]

[0419]

[0420] Example 32: Treatment Test on Pepper Anthracnose

[0421] Potted six-leaf pepper seedlings of the variety "Green Bell Pepper" were inoculated with a spore suspension of pepper anthracnose and cultured in a climate chamber. After 24 hours, they were sprayed with an aqueous solution of each active ingredient or composition (the active ingredient concentrations are described in the table below) and then moved to a greenhouse for culture. After 5-7 days, the disease was investigated after the control group had fully developed the disease.

[0422] The therapeutic activity data of the individual active components and compositions of the present invention against pepper anthracnose are shown in Tables 65 and 66.

[0423] The results in Tables 65 and 66 below show that the observed efficacy of the composition (C obs ) and expected effectiveness (C exp ) ratios are all >1, indicating that the composition exhibits a synergistic effect on pepper anthracnose within the test ratio range.

[0424] Table 65 The therapeutic effects of the active ingredients and the synergistic effects of the composition

[0425]

[0426] Table 66 The therapeutic effects of the active ingredients and the synergistic effects of the composition

[0427]

[0428]

[0429] Example 33 Test on the effectiveness of preventing and controlling pepper powdery mildew

[0430] Potted six-leaf pepper seedlings of the "Green Bell Pepper" variety were sprayed with aqueous solutions of the active ingredients or compositions (active ingredient concentrations are described in the table below). After 1, 3, 5, 7, and 10 days, spores of pepper powdery mildew were sprayed on the undersides of the leaves using an inoculator. The leaves were cultured in a greenhouse, and disease investigations were conducted 20-25 days after the control plants became fully diseased.

[0431] The activity data of each individual active ingredient and the composition of the present invention for controlling pepper powdery mildew are shown in Table 67.

[0432] The results in Table 67 below show that the observed efficacy of the individual components and the combination of the present invention against pepper powdery mildew gradually decreases with increasing time after inoculation. At the same concentration, 5 days after inoculation, the observed efficacy of the individual components is 50% lower, while the observed efficacy of the combination is greater than 60%. 10 days after inoculation, the observed efficacy of the individual components is not significant, while the observed efficacy of the combination is around 30%, demonstrating significant control efficacy. This indicates that the individual components of the present invention, when used in combination, enhance the sustained activity of the agent against pepper powdery mildew.

[0433] Table 67: Sustained activity and synergistic effects of active ingredients

[0434]

[0435]

[0436] Example 34 Field Test on Controlling Cucumber Powdery Mildew

[0437] In a greenhouse, cucumbers (cultivar "Fruit Cucumber") were sprayed with aqueous solutions of the active ingredients or compositions (at the concentrations described in the table below) at the middle stage of powdery mildew disease. Fifteen days later, the disease was investigated.

[0438] The activity data of each individual active ingredient of the present invention in controlling cucumber powdery mildew are shown in Table 68.

[0439] The activity data and synergistic effect of the composition of the present invention in controlling cucumber powdery mildew are shown in Table 69.

[0440] The results in Table 69 below show that the observed efficacy of the composition (C obs ) and expected effectiveness (C exp ) ratios are all >1, indicating that the composition has a synergistic effect on cucumber powdery mildew in the field within the test ratio range.

[0441] Table 68 Activity of individual active ingredients

[0442]

[0443] Table 69 Activity and synergistic effect of the composition of the present invention

[0444] .

Claims

1. A bactericidal composition, characterized in that: The composition comprises two active components, A and B, wherein the weight ratio between active component A and active component B is 1:50-50:1; Wherein, the active component A is a compound represented by the general formula IA or its sulfate: Where: R3 is selected from hydrogen; R4 and R5 are each selected from hydrogen; R6 and R7 are each selected from hydrogen; R8 and R9 are each selected from hydrogen; R 11 Selected from 4-chloro; R 14 、R 15 、R 16 or R 17 are respectively selected from hydrogen; W is selected from hydrogen; n is 1; The active component B is selected from one or more of zineb B134, mancozeb B135, metiram B136, propineb B137, captan B147, chlorothalonil B149, picarbutrazox B163, aminopyrifen B164, and metyltetraprole B165.

2. The bactericidal composition according to claim 1, wherein: The composition comprises active component A and active component B in a weight ratio of 1:20-20:1; Wherein, the active component A is a compound represented by the general formula IA or its sulfate; Wherein, R3 is selected from hydrogen; R4 and R5 are each selected from hydrogen; R6 and R7 are each selected from hydrogen; R8 and R9 are each selected from hydrogen; R 11 Selected from 4-chloro; n is 1; R 14 、R 15 、R 16 or R 17 are respectively selected from hydrogen; W is selected from hydrogen; The active component B is selected from one or more of zineb B134, mancozeb B135, metiram B136, propineb B137, captan B147, chlorothalonil B149, picarbutrazox B163, aminopyrifen B164, and metyltetraprole B165.

3. The bactericidal composition according to claim 2, characterized in that: The composition comprises active component A and active component B in a weight ratio of 1:10-10:1; Wherein, the active component A is selected from the compound of formula IA or its sulfate; Wherein, R3 is selected from hydrogen; R4 and R5 are each selected from hydrogen; R8 and R9 are each selected from hydrogen; R 11 Selected from 4-chloro; n is 1; R 14 、R 15 、R 16 or R 17 are respectively selected from hydrogen; W is selected from hydrogen; The active component B is selected from one or more of zineb B134, mancozeb B135, metiram B136, propineb B137, captan B147, chlorothalonil B149, picarbutrazox B163, aminopyrifen B164, and metyltetraprole B165.

4. Use of the bactericidal composition according to claim 1, characterized in that: The bactericidal composition is used for preparing medicines for preventing and treating cucumber powdery mildew, melon powdery mildew, cucumber downy mildew, grape downy mildew, sunflower downy mildew, tomato late blight, pear black spot, peanut leaf spot and rice bacterial blight.

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