A hydrogel with antibacterial and promoting granulation tissue growth and a preparation method thereof

Abstract

The present application provides a kind of hydrogel with bacteriostatic and promote granulation tissue growth, the hydrogel includes the following weight parts raw materials: saussurea extract 8-16 parts, safflower extract 20-40 parts, cnidium 8-15 parts, sophora flavescens extract 6-13 parts, angelica 5-10 parts, kangi extract 6-12 parts, aloe extract 20-30 parts, polydopamine 45-65 parts, carbomer 18-36 parts, chitosan 30-40 parts, sodium alginate 16-24 parts, collagen 20-32 parts, ginsenoside 10-19 parts, lactoferrin 18-24 parts, silk fibroin 15-20 parts, polyethylene glycol 10-16 parts.In the present application, through the mutual synergistic effect of saussurea extract, safflower extract, cnidium, sophora flavescens extract, angelica, kangi extract, aloe extract and other components, the effect of efficient and stable bacteriostatic, antipruritic, detumescence, analgesic, promoting granulation tissue growth is achieved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of hydrogel, in particular to a hydrogel with bacteriostatic and granulation tissue growth promoting functions and a preparation method thereof. BACKGROUND

[0002] Gynecological inflammation is easily caused by trichomonas, bacterial, viral infection and physical, chemical stimulation damage and other factors. Vaginal itching, vaginitis, cervicitis and uterine erosion are common gynecological diseases, which are prone to recurrence and cause great distress to women. At present, the treatment of gynecological diseases is mainly by using antibacterial and anti-inflammatory treatment lotions, suppositories, gels and the like, which can have certain bacteriostatic, antipruritic and healing promoting effects during use, but the effect is not ideal, and the gynecological diseases are prone to recurrence. In addition, some gel drugs have low adsorption, uneven drug effect and poor stability during use, so that the hydrogel drug has problems of short drug retention time, slow effect and low cure rate. SUMMARY

[0003] In view of this, the present application provides a hydrogel with bacteriostatic and granulation tissue growth promoting functions and a preparation method thereof.

[0004] The technical scheme of the present application is as follows:

[0005] A hydrogel with bacteriostatic and granulation tissue growth promoting functions comprises the following raw materials in parts by weight: 8-16 parts of saussurea involucrata extract, 20-40 parts of safflower extract, 8-15 parts of cnidium monnieri, 6-13 parts of sophora flavescens extract, 5-10 parts of angelica sinensis, 6-12 parts of polygonum hydropiper extract, 20-30 parts of aloe extract, 45-65 parts of polydopamine, 18-36 parts of carbomer, 30-40 parts of chitosan, 16-24 parts of sodium alginate, 20-32 parts of collagen, 10-19 parts of ginsenoside, 18-24 parts of lactoferrin, 15-20 parts of silk fibroin and 10-16 parts of polyethylene glycol.

[0006] Further, the preparation method of the saussurea involucrata extract is as follows: saussurea involucrata fine powder and ethanol solution with a mass concentration of 50-60% are taken in a mass ratio of 1:12-18, ultrasonic extraction is carried out at an ultrasonic power of 300-600 W for 20-30 min, the filtrate is filtered and concentrated to extract by a rotary evaporator, and then dried and crushed to obtain the saussurea involucrata extract.

[0007] Further, the preparation method of the safflower extract is as follows: the safflower flowers, safflower seeds and ethanol solution are crushed in a crusher, then ultrasonic extraction is carried out, the filter residue is added with the same concentration of ethanol solution for repeated extraction for 2-3 times, the filtrate is combined, then ε-polylysine and alginic acid propylene glycol ester are added and stirred to mix uniformly, and then concentrated to obtain the safflower extract.

[0008] Further, the safflower flower, safflower seed, ethanol solution, ε-polylysine, alginic acid propylene glycol ester mass ratio is 2-4:1-2:10-14:6-8:3-5; the mass concentration of the ethanol solution is 20-30%.

[0009] Further, the preparation method of the sophora flavescens extract is as follows: sophora flavescens powder is taken, 4-6 times water by weight is added, and the mixture is soaked at 30-40 DEG C for 3-4 h, and then decocted for 0.5-1.0 h, filtered, the residue is added with 3-5 times water, and the mixture is soaked and decocted for 1-2 h, the operation is repeated for 2-3 times, all the filtrates are combined, and concentrated to extract by rotary evaporation, dried, and crushed to obtain the sophora flavescens extract.

[0010] Further, the preparation method of the sophora flavescens extract is as follows: sophora flavescens powder is taken, 4-6 times water by weight is added, and the mixture is soaked at 30-40 DEG C for 3-4 h, and then decocted for 0.5-1.0 h, filtered, the residue is added with 3-5 times water, and the mixture is soaked and decocted for 1-2 h, the operation is repeated for 2-3 times, all the filtrates are combined, and concentrated to extract by rotary evaporation, dried, and crushed to obtain the sophora flavescens extract.

[0011] Further, the preparation method of the sophora flavescens extract is as follows: sophora flavescens powder is taken, 4-6 times water by weight is added, and the mixture is soaked at 30-40 DEG C for 3-4 h, and then decocted for 0.5-1.0 h, filtered, the residue is added with 3-5 times water, and the mixture is soaked and decocted for 1-2 h, the operation is repeated for 2-3 times, all the filtrates are combined, and concentrated to extract by rotary evaporation, dried, and crushed to obtain the sophora flavescens extract.

[0012] The preparation method of the hydrogel with antibacterial and promoting granulation tissue growth comprises the following steps:

[0013] (I): cnidium monnieri and angelica sinensis are mixed and crushed, then ethanol solution is added for ultrasonic extraction, the filtrate is concentrated to extract by rotary evaporation, and a mixture is obtained;

[0014] (II): chitosan is added to deionized water to prepare a chitosan solution; polydopamine is added to an alkaline aqueous solution with pH 8-10 and stirred to mix uniformly, then lactoferrin, silk fibroin, and the chitosan solution are added and stirred to mix uniformly, filtered, and washed with deionized water to obtain a composite gel material;

[0015] (III): the mixture prepared in the above (I) is added into 6-8 times of deionized water of the mass of the mixture, and then the aloe extract, carbomer, sodium alginate, collagen, ginsenoside, stirring and mixing are uniformly added, and then the saussurea extract, safflower extract, sophora flavescens extract, kangling extract, polyethylene glycol are stirred and mixed uniformly, and finally the composite gel material prepared in the above step (II) is added, and stirring and mixing are uniformly carried out, so that the hydrogel is obtained.

[0016] Further, in the above step (I), the mass of the ethanol solution is 8-12 times of the mass of the crushed material; the mass concentration of the ethanol solution is 60-80%; the ultrasonic extraction power is 600-800W, the temperature is 45-55℃, and the time is 60-80min.

[0017] Further, in the above step (II), the concentration of the chitosan solution is 15-20% of the mass concentration; in the above step (II), the stirring speed is 700-1500r / min, the temperature is 28-50℃, and the time is 10-20min; in the above step (III), the stirring speed is 500-1200r / min, the temperature is 28-50℃, and the time is 8-16min.

[0018] Compared with the prior art, the present application has the following beneficial effects:

[0019] 1. In the present application, the mutual synergistic effect of saussurea extract, safflower extract, corydalis, sophora flavescens extract, angelica, kangling extract, aloe extract and other components plays a role in efficient and stable antibacterial, antipruritic, detumescence, analgesic and promotion of granulation tissue growth. The synergistic effect of saussurea extract, sophora flavescens extract, angelica, aloe extract, lactoferrin and collagen can improve the broad-spectrum antibacterial property of the hydrogel product of the present application, and the antibacterial effect is good and is not easy to recur; corydalis and kangling extract have a stronger insecticidal and antipruritic effect; angelica, collagen, lactoferrin and chitosan can promote wound healing, tissue regeneration and thus promote granulation tissue growth.

[0020] 2. In the preparation process of the hydrogel of the present application, the polydopamine is compounded with lactoferrin, silk fibroin and chitosan to prepare a composite gel material, which can effectively improve the flexibility, stability and adsorption force of the hydrogel of the present application, so that the active ingredients in the hydrogel can quickly act on the target position, the effect is fast, the action time is long, and the healing effect is effectively improved; the aloe extract, carbomer and other gel components are stirred with collagen, ginsenoside and other components, and then compounded with saussurea extract, safflower extract and other components, which is beneficial to improve the uniform distribution and stability of the components in the hydrogel of the present application, improve the antibacterial property and promote the growth of granulation tissue.

[0021] 3、In the process of preparing safflower extract, the present application takes safflower flowers and safflower seeds, which can enhance the anti-inflammatory and analgesic effects of the prepared safflower extract and promote the healing of granulation tissue. In the preparation process, the addition of ε-polylysine and alginic acid propylene glycol ester, which has hydrophilic and lipophilic properties, can effectively adsorb the active substances of safflower flowers and safflower seeds, thereby improving the extraction effect and the stability of safflower extract. In addition, it can also make the safflower extract more quickly penetrate into the target position after being compounded with other raw materials during the preparation of hydrogel in the later stage, thereby promoting antibacterial and healing and improving the effect. DETAILED DESCRIPTION

[0022] In order to better understand the technical content of the present application, specific examples are provided below to further illustrate the present application.

[0023] The experimental methods used in the embodiments of the present application are conventional methods unless otherwise specified.

[0024] The materials, reagents, etc. used in the embodiments of the present application can be obtained from commercial channels unless otherwise specified.

[0025] Example 1

[0026] The hydrogel with antibacterial and granulation tissue growth promoting effect of the present embodiment comprises the following raw materials in parts by weight: safflower extract 8 parts, safflower extract 20 parts, corydalis 8 parts, sophora flavescens extract 6 parts, angelica 5 parts, kressa extract 6 parts, aloe extract 20 parts, polydopamine 45 parts, carbomer 18 parts, chitosan 30 parts, sodium alginate 16 parts, collagen 20 parts, ginsenoside 10 parts, lactoferrin 18 parts, silk fibroin 15 parts, and polyethylene glycol 10 parts.

[0027] The preparation method of the safflower extract is as follows: safflower flowers and safflower seeds are taken according to a mass ratio of 2:1, and then ultrasonic extraction is performed for 20 minutes under an ultrasonic power of 300 W. The filtrate is obtained by filtration, and then rotary evaporation is performed to concentrate the filtrate to a extract. The extract is dried and pulverized to obtain the safflower extract.

[0028] The preparation method of the safflower extract is as follows: safflower flowers and safflower seeds are taken according to a mass ratio of 2:1, and then ultrasonic extraction is performed for 20 minutes under an ultrasonic power of 300 W. The filtrate is obtained by filtration, and then rotary evaporation is performed to concentrate the filtrate to a extract. The extract is dried and pulverized to obtain the safflower extract.

[0029] The preparation method of the Sophora flavescens extract is as follows: Sophora flavescens powder is added with water in a weight ratio of 4:1, and then soaked at 30 DEG C for 3 hours and decocted for 0.5 hours; the filtrate is obtained by filtration, and the residue is added with water in a weight ratio of 3:1, and then soaked and decocted for 1 hour; the above operation is repeated twice, and all the filtrates are combined and concentrated to extract by a rotary evaporator; and the extract is dried and crushed to obtain the Sophora flavescens extract.

[0030] The preparation method of the Jialiao extract is as follows: fresh aloe is crushed to obtain aloe crushed material; the aloe crushed material and an ethanol solution with a mass concentration of 40% are taken in a mass ratio of 1:15, and then ultrasonic extraction is carried out at an ultrasonic power of 500 W for 25 min; the filtrate is obtained by filtration, and concentrated to extract by a rotary evaporator; and the extract is dried and crushed to obtain the Jialiao extract.

[0031] The preparation method of the Sophora flavescens extract is as follows: Sophora flavescens powder is added with water in a weight ratio of 4:1, and then soaked at 30 DEG C for 3 hours and decocted for 0.5 hours; the filtrate is obtained by filtration, and the residue is added with water in a weight ratio of 3:1, and then soaked and decocted for 1 hour; the above operation is repeated twice, and all the filtrates are combined and concentrated to extract by a rotary evaporator; and the extract is dried and crushed to obtain the Sophora flavescens extract.

[0032] The preparation method of the hydrogel with antibacterial and granulation tissue growth promoting functions comprises the following steps:

[0033] (I): Sophora flavescens and Angelica sinensis are mixed and crushed to obtain crushed material; the crushed material and an ethanol solution are taken in a mass ratio of 1:8, and then an ethanol solution with a mass concentration of 60% is added and ultrasonic extraction is carried out at a power of 60 W and a temperature of 45 DEG C for 60 min; the filtrate is obtained by filtration and concentrated to extract by a rotary evaporator to obtain a mixture;

[0034] (II): chitosan is added to deionized water to prepare a chitosan solution with a mass concentration of 15%; polydopamine is added to an alkaline aqueous solution with a pH of 8-10, and stirred and mixed uniformly at a speed of 1000 r / min and a temperature of 30 DEG C for 10 min; then lactoferrin and silk fibroin are added to the chitosan solution, and stirred and mixed uniformly at a speed of 700 r / min and a temperature of 40 DEG C for 10 min; the mixture is filtered and washed with deionized water to obtain a composite gel material;

[0035] (III): the mixture prepared in the above (I) is added to deionized water in a mass ratio of 6:1, and then stirred and mixed uniformly; aloe extract, carbomer, sodium alginate, collagen, ginsenoside, saussurea involucrata extract, safflower extract, Sophora flavescens extract, and Jialiao extract are added and stirred and mixed uniformly at a speed of 900 r / min and a temperature of 35 DEG C for 10 min; finally, the composite gel material prepared in the above step (II) is added and stirred and mixed uniformly at a speed of 500 r / min and a temperature of 28 DEG C for 8 min to obtain the hydrogel.

[0036] Example 2

[0037] The hydrogel with antibacterial and promoting granulation tissue growth of the present embodiment comprises the following raw materials in parts by weight: saussurea extract 16 parts, safflower extract 40 parts, cnidium 15 parts, sophora flavescens extract 13 parts, angelica 10 parts, koleria extract 12 parts, aloe extract 30 parts, polydopamine 65 parts, carbomer 36 parts, chitosan 40 parts, sodium alginate 24 parts, collagen 32 parts, ginsenoside 19 parts, lactoferrin 24 parts, silk fibroin 20 parts, polyethylene glycol 16 parts;

[0038] The preparation method of the saussurea extract is as follows: take saussurea fine powder and ethanol solution with a mass concentration of 60% according to a mass ratio of 1:18, ultrasonic extraction is carried out at an ultrasonic power of 600 W for 30 min, the filtrate is filtered and concentrated to extract by a rotary evaporator, and then dried and crushed to obtain the saussurea extract;

[0039] The preparation method of the safflower extract is as follows: take safflower flowers, safflower seeds, ethanol solution, ε-polylysine and alginic acid propylene glycol according to a mass ratio of 4:2:14:8:5, wherein the mass concentration of the ethanol solution is 30%; the safflower flowers, safflower seeds and ethanol solution are crushed in a crusher, then ultrasonic extraction is carried out, the residue is filtered, the same concentration of ethanol solution is added to the residue for repeated extraction for 3 times, then ε-polylysine and alginic acid propylene glycol ester are added and stirred to obtain the safflower extract;

[0040] The preparation method of the sophora flavescens extract is as follows: take sophora flavescens powder, add 6 times the weight of water, soak at 40℃ for 4h, and decoct for 1.0h, filter, add 5 times the weight of water to the residue, soak and decoct for 2h, repeat the operation for 3 times, combine all the filtrates, concentrate to extract by a rotary evaporator, dry and crush to obtain the sophora flavescens extract;

[0041] The preparation method of the koleria extract is as follows: take koleria powder, add 6 times the weight of water, soak at 40℃ for 4h, and decoct for 1.0h, filter, add 5 times the weight of water to the residue, soak and decoct for 2h, repeat the operation for 3 times, combine all the filtrates, concentrate to extract by a rotary evaporator, dry and crush to obtain the koleria extract;

[0042] The preparation method of the aloe extract is as follows: crush fresh aloe to obtain aloe crushed material, take the aloe crushed material and ethanol solution with a mass concentration of 60% according to a mass ratio of 1:20, ultrasonic extraction is carried out at an ultrasonic power of 700 W for 30 min, the filtrate is filtered and concentrated to extract by a rotary evaporator, and then dried and crushed to obtain the aloe extract;

[0043] The preparation method of the hydrogel with antibacterial and promoting granulation tissue growth comprises the following steps:

[0044] (I): Cnidium Monnieri and Angelica sinensis were mixed and crushed to obtain crushed materials, and then 80% ethanol solution was added to the crushed materials at a mass ratio of 1:12, and ultrasonic extraction was carried out at a power of 800 W and a temperature of 55℃ for 80 min. The filtrate was concentrated by rotary evaporation to obtain a mixture;

[0045] (II): Chitosan was added to deionized water to prepare a chitosan solution with a mass concentration of 20%; polydopamine was added to an alkaline aqueous solution with a pH of 10, and stirred at a speed of 1500 r / min and a temperature of 50℃ for 20 min, then lactoferrin, silk fibroin and chitosan solution were added and stirred at a speed of 800 r / min and a temperature of 30℃ for 15 min, and then filtered and washed with deionized water to obtain a composite gel material;

[0046] (III): The mixture prepared in the above (I) was added to 8 times its mass of deionized water and mixed uniformly, then aloe extract, carbomer, sodium alginate, collagen, ginsenoside, and polyethylene glycol were added and stirred at a speed of 1200 r / min and a temperature of 50℃ for 16 min, and finally the composite gel material prepared in the above step (II) was added and stirred at a speed of 800 r / min and a temperature of 40℃ for 14 min to obtain a hydrogel.

[0047] Example 3

[0048] The hydrogel with antibacterial and granulation tissue growth promoting properties of the present embodiment comprises the following raw materials in parts by weight: saussurea involucrata extract 12 parts, safflower extract 25 parts, cnidium monnieri 10 parts, sophora flavescens extract 8 parts, angelica sinensis 8 parts, kangling extract 10 parts, aloe extract 25 parts, polydopamine 60 parts, carbomer 22 parts, chitosan 38 parts, sodium alginate 20 parts, collagen 25 parts, ginsenoside 12 parts, lactoferrin 20 parts, silk fibroin 28 parts, and polyethylene glycol 12 parts.

[0049] The preparation method of saussurea involucrata extract is as follows: saussurea involucrata fine powder and 52% ethanol solution were taken at a mass ratio of 1:14, and ultrasonic extraction was carried out at an ultrasonic power of 400 W for 22 min. The filtrate was concentrated by rotary evaporation to obtain a extract, which was dried and crushed to obtain saussurea involucrata extract.

[0050] The preparation method of the safflower extract is as follows: safflower flowers, safflower seeds, ethanol solution, epsilon-polylysine and alginic acid propylene glycol are weighed according to the mass ratio of 2:2:11:7:5, wherein the mass concentration of the ethanol solution is 27%; the safflower flowers, safflower seeds and ethanol solution are crushed in a crusher, then ultrasonic extraction is performed, filtration is performed, the filter residue is added with the same concentration of ethanol solution for repeated extraction for 2 times, the filtrate is combined, then epsilon-polylysine and alginic acid propylene glycol ester are added and stirred to be uniformly mixed, and then concentrated, so that the safflower extract is obtained;

[0051] The preparation method of the sophora flavescens extract is as follows: sophora flavescens powder is taken, 5 times the weight of water is added, and the mixture is soaked at 32 DEG C for 3.5 h, and then decocted for 1.0 h; filtration is performed, the filter residue is added with 4 times the weight of water for soaking and decocting for 1.5 h, and the operation is repeated for 3 times; all the filtrates are combined, and rotary evaporation is performed to concentrate the filtrate to a extract, which is dried and crushed to obtain the sophora flavescens extract;

[0052] The preparation method of the knotweed extract is as follows: knotweed powder is taken, 5 times the weight of water is added, and the mixture is soaked at 32 DEG C for 3.5 h, and then decocted for 0.5 h; filtration is performed, the filter residue is added with 5 times the weight of water for soaking and decocting for 2 h, and the operation is repeated for 3 times; all the filtrates are combined, and rotary evaporation is performed to concentrate the filtrate to a extract, which is dried and crushed to obtain the knotweed extract;

[0053] The preparation method of the aloe extract is as follows: fresh aloe is crushed to obtain aloe crushed material; the aloe crushed material and ethanol solution with a mass concentration of 50% are taken according to a mass ratio of 1:18, ultrasonic extraction is performed at an ultrasonic power of 600 W for 26 min, filtration is performed, the filtrate is concentrated to a extract by rotary evaporation, and the extract is dried and crushed to obtain the aloe extract;

[0054] The preparation method of the hydrogel with antibacterial and granulation tissue growth promoting functions comprises the following steps:

[0055] (I): snakegall, angelica are mixed and crushed to obtain crushed material, the mass ratio of the crushed material to ethanol solution is 1:9, then 65% ethanol solution is added, ultrasonic extraction is performed at a power of 650 W and a temperature of 50 DEG C for 65 min, the filtrate is concentrated to a extract by rotary evaporation, and the mixture is obtained;

[0056] (II): chitosan is added to deionized water to prepare a chitosan solution with a mass concentration of 18%; polydopamine is added to an alkaline aqueous solution with a pH of 9, stirred and mixed uniformly at a speed of 1400 r / min and a temperature of 30 DEG C for 10 min, then lactoferrin, silk fibroin and the chitosan solution are added, stirred and mixed uniformly at a speed of 700 r / min and a temperature of 50 DEG C for 12 min, filtered, washed with deionized water, and a composite gel material is obtained;

[0057] (III): the mixture prepared in the above (I) is added to 7 times its mass of deionized water and mixed uniformly, then the aloe extract, carbomer, sodium alginate, collagen, ginsenoside, stirring and mixing uniformly, then the saussurea extract, safflower extract, sophora flavescens extract, kangi extract, polyethylene glycol are added and stirred and mixed uniformly at a speed of 1000 r / min and a temperature of 40°C for 9 min, finally the composite gel material prepared in the above step (II) is added and stirred and mixed uniformly at a speed of 700 r / min and a temperature of 30°C for 12 min, to obtain the hydrogel.

[0058] Example 4

[0059] The hydrogel with antibacterial and granulation tissue growth promoting function of the present embodiment comprises the following raw materials in parts by weight: saussurea extract 14 parts, safflower extract 30 parts, cnidium 13 parts, sophora flavescens extract 10 parts, angelica 6 parts, kangi extract 7 parts, aloe extract 27 parts, polydopamine 50 parts, carbomer 30 parts, chitosan 35 parts, sodium alginate 18 parts, collagen 30 parts, ginsenoside 15 parts, lactoferrin 22 parts, silk fibroin 16 parts, and polyethylene glycol 15 parts.

[0060] The preparation method of the saussurea extract is as follows: saussurea powder and an ethanol solution with a mass concentration of 55% are taken in a mass ratio of 1:16, ultrasonic extraction is performed at an ultrasonic power of 500 W for 28 min, the filtrate is obtained by filtration, rotary evaporation is performed on the filtrate to obtain an extract, and the extract is dried and crushed to obtain the saussurea extract.

[0061] The preparation method of the safflower extract is as follows: safflower flowers, safflower seeds, an ethanol solution, ε-polylysine, and alginin propylene glycol are weighed in a mass ratio of 3:1:13:8:4, the safflower flowers, safflower seeds, and ethanol solution are crushed in a crusher, ultrasonic extraction is performed, the residue is added to the same concentration of ethanol solution for repeated extraction for 3 times, the filtrate is combined, ε-polylysine and alginin propylene glycol ester are added and stirred and mixed uniformly, and then concentrated to obtain the safflower extract.

[0062] The preparation method of the sophora flavescens extract is as follows: sophora flavescens powder is added to 6 times its weight of water, soaked at 36°C for 3.8 h, and decocted for 0.5 h, the residue is filtered, the residue is added to 3 times its weight of water, soaked and decocted for 1 h, the operation is repeated 2 times, all the filtrates are combined, rotary evaporation is performed on the filtrate to obtain an extract, and the extract is dried and crushed to obtain the sophora flavescens extract.

[0063] The preparation method of the extract of polygonum hydropiper is as follows: polygonum hydropiper powder is added into water with a weight of 4 times that of the polygonum hydropiper powder, and then soaked at 35℃ for 4 hours and decocted for 1.0 hour; the filtrate is obtained by filtration; the residue is added into water with a weight of 4 times that of the residue, and then soaked and decocted for 1 hour; the operation is repeated twice; all the filtrates are combined and concentrated into extract by a rotary evaporator; and the extract is dried and crushed to obtain the extract of polygonum hydropiper;

[0064] The preparation method of the aloe extract is as follows: fresh aloe is crushed to obtain aloe crushed material; the aloe crushed material and an ethanol solution with a mass concentration of 55% are taken according to a mass ratio of 1:16; the ultrasonic extraction is performed at an ultrasonic power of 550 W for 28 minutes; the filtrate is obtained by filtration; and the filtrate is concentrated into extract by a rotary evaporator; and the extract is dried and crushed to obtain the aloe extract.

[0065] The preparation method of the hydrogel with antibacterial and granulation tissue growth promoting functions comprises the following steps:

[0066] (1) The Cnidium monnieri and Angelica sinensis are mixed and crushed to obtain crushed materials; the crushed materials and an ethanol solution are taken according to a mass ratio of 1:10; then the 70% ethanol solution is added; and the ultrasonic extraction is performed at a power of 700 W and a temperature of 52℃ for 70 minutes; the filtrate is concentrated into extract by a rotary evaporator to obtain a mixture;

[0067] (2) Chitosan is added into deionized water to prepare a chitosan solution with a mass concentration of 16%; polydopamine is added into an alkaline aqueous solution with a pH of 10; and the stirring and mixing are uniformly performed at a rotating speed of 1200 r / min and a temperature of 40℃ for 15 minutes; then the lactoferrin and silk fibroin are added; the stirring and mixing are uniformly performed at a rotating speed of 900 r / min and a temperature of 45℃ for 18 minutes; the filtration is performed; and the composite gel material is obtained by washing with deionized water.

[0068] (3) The mixture prepared in the above (1) is added into deionized water with a mass of 6 times that of the mixture; then the aloe extract, carbomer, sodium alginate, collagen, ginsenoside, and polyethylene glycol are added and stirred and mixed uniformly at a rotating speed of 1100 r / min and a temperature of 45℃ for 10 minutes; finally, the composite gel material prepared in the above step (2) is added; and the stirring and mixing are uniformly performed at a rotating speed of 600 r / min and a temperature of 35℃ for 10 minutes to obtain the hydrogel.

[0069] Comparative Example 1

[0070] The difference between the present comparative example and Example 4 is that the present comparative example does not contain the components of polydopamine and silk fibroin, and the remaining components are the same as those of Example 4.

[0071] The hydrogel with bacteriostatic and granulation tissue growth promoting function of the embodiment comprises the following raw materials in parts by weight: saussurea extract 14 parts, safflower extract 30 parts, cnidium 13 parts, sophora flavescens extract 10 parts, angelica 6 parts, kressera extract 7 parts, aloe extract 27 parts, carbomer 30 parts, chitosan 35 parts, sodium alginate 18 parts, collagen 30 parts, ginsenoside 15 parts, lactoferrin 22 parts, and polyethylene glycol 15 parts.

[0072] The saussurea extract is prepared as follows: saussurea powder and an ethanol solution with a mass concentration of 55% are taken in a mass ratio of 1:16, ultrasonic extraction is performed at an ultrasonic power of 500 W for 28 min, the filtrate is obtained by filtration, and rotary evaporation is performed on the filtrate to concentrate the extract to a dry extract, which is dried and crushed to obtain the saussurea extract.

[0073] The preparation method of the safflower extract is as follows: safflower flowers, safflower seeds, an ethanol solution, ε-polylysine, and alginin propylene glycol are weighed in a mass ratio of 3:1:13:8:4, the ethanol solution has a mass concentration of 24%; the safflower flowers, safflower seeds, and ethanol solution are crushed in a crusher, ultrasonic extraction is performed, the filtrate is obtained by filtration, the filtrate is combined after the residue is repeatedly extracted with the same concentration of ethanol solution for three times, ε-polylysine and alginin propylene glycol ester are added and stirred to obtain the safflower extract.

[0074] The preparation method of the sophora flavescens extract is as follows: sophora flavescens powder is added with water in a weight ratio of 6:1, the mixture is soaked at 36°C for 3.8 h, and decocted for 0.5 h, the filtrate is obtained by filtration, the residue is added with water in a weight ratio of 3:1, the mixture is soaked and decocted for 1 h, the operation is repeated twice, all the filtrates are combined, rotary evaporation is performed to concentrate the extract to a dry extract, which is dried and crushed to obtain the sophora flavescens extract.

[0075] The preparation method of the kressera extract is as follows: kressera powder is added with water in a weight ratio of 4:1, the mixture is soaked at 35°C for 4 h, and decocted for 1.0 h, the filtrate is obtained by filtration, the residue is added with water in a weight ratio of 4:1, the mixture is soaked and decocted for 1 h, the operation is repeated twice, all the filtrates are combined, rotary evaporation is performed to concentrate the extract to a dry extract, which is dried and crushed to obtain the kressera extract.

[0076] The preparation method of the aloe extract is as follows: fresh aloe is crushed to obtain aloe crushed material, the aloe crushed material and an ethanol solution with a mass concentration of 55% are taken in a mass ratio of 1:16, ultrasonic extraction is performed at an ultrasonic power of 550 W for 28 min, the filtrate is obtained by filtration, and rotary evaporation is performed on the filtrate to concentrate the extract to a dry extract, which is dried and crushed to obtain the aloe extract.

[0077] The preparation method of the hydrogel with bacteriostatic and granulation tissue growth promoting function comprises the following steps:

[0078] (1): Cnidium, Angelica mixed and crushed to obtain crushed materials, then 70% ethanol solution was added to the crushed materials at a mass ratio of 1:10, and ultrasonic extraction was carried out at a power of 700W and a temperature of 52℃ for 70min. The filtrate was concentrated by rotary evaporation to obtain a mixture;

[0079] (2): Chitosan was added to deionized water to prepare a chitosan solution with a mass concentration of 16%; then lactoferrin and the chitosan solution were mixed uniformly at a stirring speed of 900r / min and a temperature of 45℃ for 18min, filtered, and washed with deionized water to obtain a composite gel material;

[0080] (3): The mixture prepared in the above (1) was added to 6 times its mass of deionized water and mixed uniformly, then aloe extract, carbomer, sodium alginate, collagen, ginsenoside, and stirring and mixing were carried out uniformly at a stirring speed of 1100r / min and a temperature of 45℃ for 10min, finally the composite gel material prepared in the above step (2) was added, and stirring and mixing were carried out uniformly at a stirring speed of 600r / min and a temperature of 35℃ for 10min, to obtain a hydrogel.

[0081] Comparative Example 2

[0082] The difference between this comparative example and Example 4 is that it does not contain saussurea extract, safflower extract, koleria extract, and aloe extract, and the remaining components are the same as those of Example 4.

[0083] The hydrogel with antibacterial and granulation tissue growth promoting properties of the present example comprises the following raw materials in parts by weight: Cnidium 13 parts, Sophora flavescens extract 10 parts, Angelica 6 parts, polydopamine 50 parts, carbomer 30 parts, chitosan 35 parts, sodium alginate 18 parts, collagen 30 parts, ginsenoside 15 parts, lactoferrin 22 parts, silk fibroin 16 parts, and polyethylene glycol 15 parts.

[0084] The preparation method of Sophora flavescens extract is as follows: Sophora flavescens powder is added to 6 times its weight of water, soaked at 36℃ for 3.8h, and decocted for 0.5h, the filtrate is filtered, the filter residue is added to 3 times its weight of water, soaked and decocted for 1h, and the operation is repeated twice, all the filtrates are combined and concentrated by rotary evaporation to obtain an extract, which is dried and crushed to obtain Sophora flavescens extract.

[0085] The preparation method of the hydrogel with antibacterial and granulation tissue growth promoting properties comprises the following steps:

[0086] (1): Cnidium monnieri and Angelica sinensis were mixed and crushed to obtain crushed materials, and then 70% ethanol solution was added to the crushed materials at a mass ratio of 1:10, and ultrasonic extraction was carried out at a power of 700 W and a temperature of 52℃ for 70 min. The filtrate was concentrated by rotary evaporation to obtain a mixture;

[0087] (2): Chitosan was added to deionized water to prepare a chitosan solution with a mass concentration of 16%; polydopamine was added to an alkaline aqueous solution with a pH of 10, and stirred at a speed of 1200 r / min and a temperature of 40℃ for 15 min, then lactoferrin, silk fibroin and chitosan solution were added and stirred at a speed of 900 r / min and a temperature of 45℃ for 18 min, and then filtered and washed with deionized water to obtain a composite gel material;

[0088] (3): The mixture prepared in the above (1) was added to 6 times its mass of deionized water and stirred evenly, then carbomer, sodium alginate, collagen, ginsenoside, bitter apricot kernel extract and polyethylene glycol were added and stirred evenly at a speed of 1100 r / min and a temperature of 45℃ for 10 min, and finally the composite gel material prepared in the above step (2) was added and stirred evenly at a speed of 600 r / min and a temperature of 35℃ for 10 min to obtain a hydrogel.

[0089] Comparative Example 3

[0090] The difference between this comparative example and Example 4 is that the preparation method of safflower extract is different, and the rest is the same.

[0091] The hydrogel with antibacterial and granulation tissue growth promoting properties of the present example comprises the following raw materials by weight: safflower extract 30 parts, cnidium monnieri 13 parts, bitter apricot kernel extract 10 parts, Angelica sinensis 6 parts, kress plant extract 7 parts, aloe extract 27 parts, polydopamine 50 parts, carbomer 30 parts, chitosan 35 parts, sodium alginate 18 parts, collagen 30 parts, ginsenoside 15 parts, lactoferrin 22 parts, silk fibroin 16 parts, and polyethylene glycol 15 parts.

[0092] The preparation method of safflower extract is as follows: safflower powder and 55% ethanol solution were taken at a mass ratio of 1:16, and ultrasonic extraction was carried out at an ultrasonic power of 500 W for 28 min. The filtrate was concentrated by rotary evaporation to obtain a mixture, which was dried and crushed to obtain safflower extract.

[0093] The preparation method of the safflower extract is as follows: safflower flowers and an ethanol solution are taken in a mass ratio of 1:10, wherein the mass concentration of the ethanol solution is 24%; the safflower flowers are crushed in a crusher, and then the ethanol solution is added for ultrasonic extraction; the filtrate is obtained by filtration; the filtrate is combined with the filtrate obtained by repeating the extraction of the filtrate residue with the same concentration of ethanol solution for three times; and the combined filtrate is concentrated to obtain the safflower extract.

[0094] The preparation method of the sophora flavescens extract is as follows: sophora flavescens powder is taken and soaked in water at a weight ratio of 1:6 at 36 DEG C for 3.8 h; the sophora flavescens powder is decocted for 0.5 h; the filtrate is obtained by filtration; the filtrate residue is soaked in water at a weight ratio of 1:3 for 1 h; the operation is repeated twice; all the filtrates are combined; the combined filtrate is concentrated to a decoction by a rotary evaporator; and the sophora flavescens extract is obtained by drying and crushing.

[0095] The preparation method of the klamath weed extract is as follows: klamath weed powder is taken and soaked in water at a weight ratio of 1:4 at 35 DEG C for 4 h; the klamath weed powder is decocted for 1.0 h; the filtrate is obtained by filtration; the filtrate residue is soaked in water at a weight ratio of 1:4 for 1 h; the operation is repeated twice; all the filtrates are combined; the combined filtrate is concentrated to a decoction by a rotary evaporator; and the klamath weed extract is obtained by drying and crushing.

[0096] The preparation method of the aloe extract is as follows: fresh aloe is crushed to obtain aloe crushed material; the aloe crushed material and an ethanol solution with a mass concentration of 55% are taken in a mass ratio of 1:16; the aloe crushed material is ultrasonically extracted for 28 min at an ultrasonic power of 550 W; the filtrate is obtained by filtration; the filtrate is concentrated to a decoction by a rotary evaporator; and the aloe extract is obtained by drying and crushing.

[0097] The preparation method of the hydrogel with antibacterial and granulation tissue growth promoting functions comprises the following steps:

[0098] (I): snakegall and angelica are mixed and crushed to obtain crushed material; the crushed material and an ethanol solution are taken in a mass ratio of 1:10; then the crushed material is ultrasonically extracted in an ethanol solution with a mass concentration of 70% at a power of 700 W and a temperature of 52 DEG C for 70 min; the filtrate is concentrated to a decoction by a rotary evaporator to obtain a mixture;

[0099] (II): chitosan is added to deionized water to prepare a chitosan solution with a mass concentration of 16%; polydopamine is added to an alkaline aqueous solution with a pH of 10; the mixture is stirred at a speed of 1200 r / min and a temperature of 40 DEG C for 15 min; then lactoferrin and silk fibroin are added to the chitosan solution; the mixture is stirred at a speed of 900 r / min and a temperature of 45 DEG C for 18 min; the mixture is filtered and washed with deionized water to obtain a composite gel material;

[0100] (III): the mixture prepared in (I) above is added to 6 times its mass of deionized water and mixed uniformly, then the aloe extract, carbomer, sodium alginate, collagen, ginsenoside, stirring and mixing uniformly, then the saussurea extract, safflower extract, sophora flavescens extract, kangi-persic extract, polyethylene glycol are added and stirred and mixed uniformly at a rotation speed of 1100 r / min and a temperature of 45°C for 10 min, and finally the composite gel material prepared in step (II) above is added and stirred and mixed uniformly at a rotation speed of 600 r / min and a temperature of 35°C for 10 min, to obtain the hydrogel.

[0101] Experimental process:

[0102] The hydrogels prepared in Examples 1-4 and Comparative Examples 1-3 of the present application are subjected to experiments. The specific experimental conditions are shown below.

[0103] (1) Gel adhesion test: two pieces of fresh rabbit vaginal tissue of the same area S are taken, one end of each piece is fixed to a balance, the other end is fixed, the mucosa faces outward, and is kept at 38°C for 15 min. Then the same 0.1 g of the hydrogel prepared in each of the examples and each of the comparative examples is evenly applied to the rabbit vaginal tissue, so that the two pieces of vaginal tissue are tightly adhered, and then a weight is added to the other end of the balance to separate the two pieces of adhered vaginal tissue, and the weight of the weight is recorded as M. The adhesion of the gel is the degree of peeling per unit area, i.e. the peeling force = 0.98M / S (dyne / cm 2 ), each experiment of Examples 1-4 and Comparative Examples 1-3 is repeated 3 times, and the average value is taken.

[0104] (2) Bacteriostatic test

[0105] The bacteriostatic diameter is measured by the Oxford cup double-layer plate method, with a dose of 50 μL / cup. The fungi are cultured on experimental plates for 48 h, and the bacteria are cultured on experimental plates for 24 h. The test sample is the hydrogel product prepared in Examples 1-4 and Comparative Examples 1-3 of the present application, and the control group is treated with the same amount of normal saline. The bacteriostatic group is Examples 1-4 and Comparative Examples 1-3 of the present application, and 8 Oxford cups are used to calculate the bacteriostatic rate for each test group, and the experimental results are averaged. The calculation formula is:

[0106] Bacteriostatic rate (%) = Bacteriostatic group bacterial circle diameter (mm) / Control group bacterial circle diameter (mm) x 100%

[0107] (3) Granulation tissue growth test

[0108] Take 20±2g of healthy female Kunming mice 80, after being anesthetized with anesthetic, use a sterilized knife to draw a 1cm×1cm bleeding wound on the same side of the mouse's labia outside the vagina, and after stopping bleeding, naturally feed, do not bandage treatment to make it infected, inflamed and pustulated. The 80 mice infected with pustules are randomly divided into 10 groups, 10 in each group, corresponding to the control group, examples 1-4 and comparative examples 1-3. On the second day of infection, each group is given the same amount of hydrogel prepared by examples 1-4 and comparative examples 1-3 for smearing treatment, and the control group is treated with normal saline. Smear 2 times in the morning and afternoon every day, and observe the granulation tissue of the wound after 5 days of smearing, and record the number of mice in the corresponding group. The wound site has scab growth, no purulent secretion, and the central granulation of the wound is uniform and bright red.

[0109] Table 1 Hydrogel test conditions

[0110]

[0111] The above results show that examples 1-4 and comparative examples 1-3 have good antibacterial and granulation tissue growth promoting effects. And the adhesion of the composite hydrogel prepared without polydopamine and silk protein components is significantly reduced, indicating that the mutual synergistic effect of polydopamine and silk protein can enhance the adhesion of the hydrogel and promote the antibacterial effect. In addition, the liquid prepared by mixing the flowers and seeds of safflower can significantly enhance the antibacterial effect and promote the growth of granulation tissue. The components of snow lotus extract, safflower extract, kressa extract and aloe extract have some influence on the adhesion, antibacterial effect and growth of granulation tissue of the hydrogel, but the influence is less than that of polydopamine, silk protein and safflower extract.

[0112] The above only describes the preferred embodiments of the present application and does not limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A hydrogel having antibacterial and promoting granulation tissue growth, characterized in that, The patent application discloses a preparation method of the extract of saussurea involucrata, which comprises the following steps: taking saussurea involucrata powder and an ethanol solution with a mass concentration of 50-60% according to a mass ratio of 1:12-18, and performing ultrasonic extraction under an ultrasonic power of 300-600 W for 20-30 min; filtering to obtain a filtrate; and rotating and concentrating the filtrate to extract residue by using a rotary evaporator, drying, and crushing to obtain the extract of saussurea involucrata.

2. The hydrogel with antibacterial and granulation tissue growth promoting properties according to claim 1, characterized in that, The preparation method of the safflower extract is as follows: safflower flowers, safflower seeds and an ethanol solution are crushed in a crusher, then ultrasonic extraction is performed, the filter residue is added into the same concentration of ethanol solution for repeated extraction for 2-3 times, the filtrates are combined, then ε-polylysine and alginic acid propylene glycol ester are added and uniformly stirred and mixed, and then concentrated to obtain the safflower extract.

3. The hydrogel with antibacterial and granulation tissue growth promoting properties according to claim 1, wherein, The mass ratio of the safflower flowers, the safflower seeds, the ethanol solution, the ε-polylysine, the alginic acid propylene glycol ester is 2-4:1-2:10-14:6-8:3-5; and the mass concentration of the ethanol solution is 20-30%.

4. The hydrogel with antibacterial and granulation tissue growth promoting properties according to claim 3, characterized in that, The preparation method of the extract of radix sophorae flavescentis is as follows: radix sophorae flavescentis powder is added into water with a weight of 4-6 times of the radix sophorae flavescentis powder, and then soaked at 30-40 DEG C for 3-4 h, and then decocted for 0.5-1.0 h; the filter residue is added into water with a weight of 3-5 times of the water, and then soaked and decocted for 1-2 h; the operation is repeated for 2-3 times; all the filtrates are combined, and then concentrated to extract residue by using a rotary evaporator; and then dried and crushed to obtain the extract of radix sophorae flavescentis.

5. The hydrogel with antibacterial and granulation tissue growth promoting properties according to claim 1, wherein, The preparation method of the extract of radix sophorae flavescentis is as follows: radix sophorae flavescentis powder is added into water with a weight of 4-6 times of the radix sophorae flavescentis powder, and then soaked at 30-40 DEG C for 3-4 h, and then decocted for 0.5-1.0 h; the filter residue is added into water with a weight of 3-5 times of the water, and then soaked and decocted for 1-2 h; the operation is repeated for 2-3 times; all the filtrates are combined, and then concentrated to extract residue by using a rotary evaporator; and then dried and crushed to obtain the extract of radix sophorae flavescentis.

6. The hydrogel with antibacterial and granulation tissue growth promoting properties according to claim 1, wherein, The preparation method of the aloe extract is as follows: fresh aloe is crushed to obtain aloe crushed material; the aloe crushed material and an ethanol solution with a mass concentration of 40-60% are taken according to a mass ratio of 1:15-20, and then ultrasonic extraction is performed under an ultrasonic power of 500-700 W for 25-30 min; the filtrate is obtained by filtering; and the filtrate is concentrated to extract residue by using a rotary evaporator, dried, and crushed to obtain the aloe extract.

7. The hydrogel with antibacterial and granulation tissue growth promoting properties according to claim 1, wherein, The preparation method of the extract of radix sophorae flavescentis is as follows: radix sophorae flavescentis powder is added into water with a weight of 4-6 times of the radix sophorae flavescentis powder, and then soaked at 30-40 DEG C for 3-4 h, and then decocted for 0.5-1.0 h; the filter residue is added into water with a weight of 3-5 times of the water, and then soaked and decocted for 1-2 h; the operation is repeated for 2-3 times; all the filtrates are combined, and then concentrated to extract residue by using a rotary evaporator; and then dried and crushed to obtain the extract of radix sophorae flavescentis.

8. The method for preparing a hydrogel with antibacterial and granulation tissue-promoting effects according to any one of claims 1 to 7, characterized in that, The preparation method of the extract of radix sophorae flavescentis is as follows: radix sophorae flavescentis powder is added into water with a weight of 4-6 times of the radix sophorae flavescentis powder, and then soaked at 30-40 DEG C for 3-4 h, and then decocted for 0.5-1.0 h; the filter residue is added into water with a weight of 3-5 times of the water, and then soaked and decocted for 1-2 h; the operation is repeated for 2-3 times; all the filtrates are combined, and then concentrated to extract residue by using a rotary evaporator; and then dried and crushed to obtain the extract of radix sophorae flavescentis. The preparation method of the aloe extract is as follows: fresh aloe is crushed to obtain aloe crushed material; the aloe crushed material and an ethanol solution with a mass concentration of 40-60% are taken according to a mass ratio of 1:15-20, and then ultrasonic extraction is performed under an ultrasonic power of 500-700 W for 25-30 min; the filtrate is obtained by filtering; and the filtrate is concentrated to extract residue by using a rotary evaporator, dried, and crushed to obtain the aloe extract. The preparation method of the extract of radix sophorae flavescentis is as follows: radix sophorae flavescentis powder is added into water with a weight of 4-6 times of the radix sophorae flavescentis powder, and then soaked at 30-40 DEG C for 3-4 h, and then decocted for 0.5-1.0 h; the filter residue is added into water with a weight of 3-5 times of the water, and then soaked and decocted for 1-2 h; the operation is repeated for 2-3 times; all the filtrates are combined, and then concentrated to extract residue by using a rotary evaporator; and then dried and crushed to obtain the extract of radix sophorae flavescentis. The preparation method of the aloe extract is as follows: fresh aloe is crushed to obtain aloe crushed material; the aloe crushed material and an ethanol solution with a mass concentration of 40-60% are taken according to a mass ratio of 1:15-20, and then ultrasonic extraction is performed under an ultrasonic power of 500-700 W for 25-30 min; the filtrate is obtained by filtering; and the filtrate is concentrated to extract residue by using a rotary evaporator, dried, and crushed to obtain the aloe extract. The preparation method of the extract of radix sophorae flavescentis is as follows: radix sophorae flavescentis powder is added into water with a weight of 4-6 times of the radix sophorae flavescentis powder, and then soaked at 30-40 DEG C for 3-4 h, and then decocted for 0.5-1.0 h; the filter residue is added into water with a weight of 3-5 times of the water, and then soaked and decocted for 1-2 h; the operation is repeated for 2-3 times; all the filtrates are combined, and then concentrated to extract residue by using a rotary evaporator; and then dried and crushed to obtain the extract of radix sophorae flavescentis. The preparation method of the aloe extract is as follows: fresh aloe is crushed to obtain aloe crushed material; the aloe crushed material and an ethanol solution with a mass concentration of 40-60% are taken according to a mass ratio of 1:15-20, and then ultrasonic extraction is performed under an ultrasonic power of 500-700 W for 25-30 min; the filtrate is obtained by filtering; and the filtrate is concentrated to extract residue by using a rotary evaporator, dried, and crushed to obtain the aloe extract. The preparation method of the extract of radix sophorae flavescentis is as follows: radix sophorae flavescentis powder is added into water with a weight of 4-6 times of the radix sophorae flavescentis powder, and then soaked at 30-40 DEG C for 3-4 h, and then decocted for 0.5-1.0 h; the filter residue is added into water with a weight of 3-5 times of the water, and then soaked and decocted for 1-2 h; the operation is repeated for 2-3 times; all the filtrates are combined, and then concentrated to extract residue by using a rotary evaporator; and then dried and crushed to obtain the extract of radix sophorae flavescentis. The preparation method of the aloe extract is as follows: fresh aloe is crushed to obtain aloe crushed material; the aloe crushed material and an ethanol solution with a mass concentration of 40-60% are taken according to a mass ratio of 1:15-20, and then ultrasonic extraction is performed under an ultrasonic power of 500 (ii) adding chitosan into deionized water to prepare a chitosan solution; adding polydopamine into an alkaline aqueous solution with pH 8-10 and stirring to mix uniformly, then adding lactoferrin, silk fibroin and the chitosan solution and stirring to mix uniformly, filtering, and washing with deionized water to obtain a composite gel material; (iii) adding the mixture prepared in (i) above into deionized water with a mass of 6-8 times that of the mixture, then adding aloe extract, carbomer, sodium alginate, collagen, ginsenoside, and stirring to mix uniformly, then adding saussurea extract, safflower extract, sophora flavescens extract, and polyethylene glycol, and stirring to mix uniformly, and finally adding the composite gel material prepared in (ii) above, and stirring to mix uniformly to obtain a hydrogel.

9. The method for preparing the hydrogel with antibacterial and granulation tissue-promoting effects according to claim 7, characterized in that, In (i) above, the mass of the ethanol solution is 8-12 times that of the crushed material; the mass concentration of the ethanol solution is 60-80%; the ultrasonic extraction power is 600-800 W, the temperature is 45-55℃, and the time is 60-80 min.

10. The method for preparing a hydrogel with antibacterial and granulation tissue-promoting properties according to claim 7, characterized in that, In (ii) above, the concentration of the chitosan solution is 15-20% by mass; in (ii) above, the stirring speed is 700-1500 r / min, the temperature is 28-50℃, and the time is 10-20 min; in (iii) above, the stirring speed is 500-1200 r / min, the temperature is 28-50℃, and the time is 8-16 min.

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