A water conditioning composition combined with bacteria and enzymes and its preparation method

Abstract

The present invention belongs to the field of water treatment technology, and specifically relates to a water quality conditioning composition combined with bacteria and enzymes and a preparation method thereof. A preparation method of a water quality conditioning composition combined with bacteria and enzymes comprises the following steps: preparing a composite bacterial agent, preparing a core material, preparing a wall material, and preparing a water quality conditioning composition combined with bacteria and enzymes. The present invention uses different photosynthetic bacteria, nitrifying bacteria, composite fungi, and composite enzymes in combination, and prepares a water quality conditioning composition combined with bacteria and enzymes that can be slowly released through emulsification and spray drying. The composition can be applied to different water bodies and effectively reduce the chemical oxygen demand (COD) and permanganate index (COD Mn ) and total phosphorus index, and can inhibit the growth of pathogens and harmful algae in water bodies. The metabolites produced by microorganisms have a good adsorption and flocculation effect on heavy metals and colloidal substances in water bodies, which can promote the balance of bacteria and algae in water bodies and is suitable for the ecological restoration of natural water bodies.

Description

Technical Field

[0001] The invention belongs to the technical field of water treatment, and particularly relates to a bacteria-enzyme combined water conditioning composition and a preparation method thereof. Background Art

[0002] Biological methods can restore polluted water environments to their original state, are low-cost, mild in effect, and free of secondary pollution, and have broad application prospects. Chinese patent CN 107082497 B discloses the use of Bacillus laterosporus, photosynthetic bacteria, Lactobacillus acidophilus, yeast, a complex enzyme solution, an adsorbent, etc. to prepare a slow-release enzyme-bacteria mixture, which has phosphorus removal, nitrogen removal, and sterilization effects on polluted water bodies; Chinese patent CN 103710284B discloses the use of Bacillus subtilis and Rhodobacter sphaeroides together to reduce ammonia nitrogen in water bodies. It can be seen that in the prior art, when using biological methods, water pollution is mainly controlled by ammonia nitrogen, total phosphorus, and chemical oxygen demand in water bodies. Among the water pollution indicators, there is also the permanganate index, which is related to reducing inorganic substances such as nitrite, ferrous salt, and sulfide in water. At the same time, there is also the problem of heavy metal pollution in water bodies. Therefore, it is necessary to further improve the existing methods to achieve the treatment and ecological restoration of polluted water bodies. Summary of the Invention

[0003] One of the purposes of the present invention is to provide a water conditioning composition combined with bacteria and enzymes, which can adapt to various water environments, remove pollutants in water, reduce chemical oxygen demand (COD), permanganate index (COD Mn ) and total phosphorus content, repairing the ecological environment of water bodies, and has great promotion and application value.

[0004] The second object of the present invention is to provide a method for preparing a water conditioning composition combined with bacteria and enzymes, which is simple and has low production cost.

[0005] In order to solve the above technical problems of the present invention, the present invention provides the following technical solutions:

[0006] The first object of the present invention is to provide a method for preparing a water conditioning composition combined with bacteria and enzymes, comprising the following steps:

[0007] S1. Preparation of a composite bacterial agent: respectively culture Rhodopseudomonas sphaeroides, Rhodobacter sphaeroides, purple non-sulfur photosynthetic bacteria, Rhodobacter capsulatus, Bacillus subtilis, iron-oxidizing bacteria, Nitrosomonas, and Nitrobacter on separate culture media to obtain corresponding bacterial liquids, centrifuge at 10,000-12,000 rpm to obtain bacterial slurry, add a protective agent, and freeze-dry to obtain bacterial powder, and mix the bacterial powders in a ratio of 1:1:1:1:1-3:1-3:0.5-1:0.5-1 to obtain a composite bacterial agent;

[0008] S2. Preparation of core material: Dissolve 80-100 parts of the composite bacterial agent obtained in S1, 20-50 parts of the composite fungal agent, and 1-5 parts of the composite enzyme in 400-500 parts of corn oil, then add 30-50 parts of modified bentonite and 1-3 parts of cetyl polyethylene glycol, and emulsify and disperse at 8000-10000 rpm at room temperature for 20-30 minutes to obtain the core material;

[0009] S3. Prepare wall material: dissolve chitosan in deionized water to obtain a chitosan solution with a mass volume ratio of 1% to 3%, which is the wall material;

[0010] S4. Prepare a bacterial-enzyme combined water conditioning composition: mix the core material obtained in S2 and the wall material obtained in S3 in a mass ratio of 4-6:1, disperse at 8000-10000 rpm for 30-40 minutes, and spray dry to obtain a bacterial-enzyme combined water conditioning composition.

[0011] Rhodobacter sphaeroides, Rhodobacter sphaeroides, purple non-sulfur photosynthetic bacteria, and Rhodobacter capsulatus are all photosynthetic bacteria that can use light as energy and carbon dioxide or small molecular organic matter in water as carbon sources to carry out complete autotrophy or photoheterotrophy. The combination of different photosynthetic bacteria and the use of various metabolic pathways in their bodies can effectively reduce the concentration of pollutants such as ammonia nitrogen, sulfide, and nitrite in water bodies, and increase the dissolved oxygen content in water bodies. At the same time, it has a good removal effect on heavy metals. At the same time, after adding these four different photosynthetic bacteria to the water body, algae such as diatoms and chlorella in the water body can become dominant algae, inhibiting the reproduction of harmful algae such as cyanobacteria. By decomposing harmful organic matter and replenishing the bacterial community, a balance between bacteria and algae is achieved, the water quality is not easy to change, the water quality can be stabilized, and the balance of the water ecosystem can be maintained.

[0012] Bacillus subtilis is an aerobic bacterium with rapid growth and relatively low nutritional requirements. It efficiently secretes a variety of proteins and metabolites without producing toxins, making it a safe, non-pathogenic microorganism. During its growth, it produces active substances such as subtilisin, polymyxin, nystatin, and gramicidin, which have a significant inhibitory effect on pathogenic bacteria in water.

[0013] Iron-oxidizing bacteria use CO2 as a carbon source and oxidize Fe 2+ to Fe 3+ The autotrophic microorganisms that obtain energy to grow can produce iron hydroxide precipitates with a flocculation effect, which can effectively absorb colloidal particles in the water, making the water clearer.

[0014] Nitrosomonas converts ammonia nitrogen into nitrite, and Nitrobacter converts nitrite into nitrate, effectively removing ammonia nitrogen from water bodies.

[0015] Furthermore, the protective agent is a mixture of 1-3% by mass glycerol, 2-4% by mass glucose solution and 0.7-0.9% by mass sodium chloride solution in a mass ratio of 1-3:1:0.5-1.

[0016] Furthermore, in S1, the amount of the protective agent used is 2-4 times the mass of the bacterial sludge.

[0017] Furthermore, in S2, the composite fungal agent is a mixture of Aspergillus niger spore powder, Trichoderma T22, and brewer's yeast powder; wherein the mass ratio of Aspergillus niger spore powder, Trichoderma T22, and brewer's yeast powder is 0.5-1:1-2:1.

[0018] Aspergillus niger spore powder is the reproductive cell of Aspergillus niger, which has strong environmental adaptability and can produce microbial flocculants such as extracellular polysaccharides. Its flocculation effect is better than traditional chemical flocculants such as polyacrylamide and polyaluminum chloride. It can effectively adsorb heavy metal pollutants in water bodies. At the same time, it can produce amylase, acid protease, cellulase, pectinase, glucose oxidase, citric acid, gluconic acid and gallic acid, and further hydrolyze various proteins, cellulose, pectin and other organic substances in the water through the catalytic action of enzymes.

[0019] Trichoderma T22 can produce small-molecule antibiotics and large-molecule antimicrobial proteins or cell wall-degrading enzymes to inhibit the growth, reproduction, and infection of pathogens. In its antibiotic and mycoparasitic activities, Trichoderma can produce chitinase, glucanase, cellulase, and protease to decompose the cell walls of plant pathogens, or secrete extracellular enzymes such as glucosidase to degrade antibiotic toxins produced by pathogens.

[0020] Brewer's yeast is a heterotrophic facultative anaerobic microorganism that can inhibit the reproduction of pathogenic bacteria and produce growth-promoting factors to promote the growth and reproduction of beneficial bacteria.

[0021] Furthermore, in S2, the complex enzyme is a mixture of dispase, humase, and saccharifying enzyme; wherein the mass ratio of dispase, humase, and saccharifying enzyme is 2-3:1:1.

[0022] Dispase can decompose organic substances such as starch, protein and fat in water bodies; humic acid is a type of organic substance accumulated after the remains of animals and plants are decomposed and transformed by microorganisms. The total amount in water bodies is large. Humic acid can be decomposed into smaller organic molecules by humase, which can fully promote nutrient circulation and activity; saccharifying enzyme can hydrolyze starch and dextrin to release glucose.

[0023] Furthermore, the preparation method of the modified bentonite is:

[0024] S11, after mixing bentonite with 5-10% acetic acid solution by mass, ultrasonically treat for 30-40 minutes, centrifuge at 5000-6000 rpm for 8-10 minutes, filter, wash the precipitate with deionized water 3-4 times, and dry at 100-120° C. for 1-2 hours to obtain pretreated bentonite;

[0025] S12. Mix the pretreated bentonite obtained in S11, anionic polyacrylamide, aluminate coupling agent, and deionized water in a mass ratio of 20-40:5-10:0.5-1:100-150, perform ultrasonic treatment for 15-20 minutes, let stand for 4-6 hours, filter, and dry to obtain modified bentonite.

[0026] After being modified, the specific surface area of ​​the bentonite can be increased, thereby enhancing its adsorption capacity and having better suspension performance, so that various microorganisms and complex enzymes in the bacterial-enzyme combined water conditioning composition are evenly dispersed and adsorbed in the pores of the modified bentonite.

[0027] Furthermore, in S11, the mass volume ratio (g:mL) of the bentonite to the acetic acid solution is 8-10:100-120.

[0028] Furthermore, in S11, the frequency of the ultrasonic treatment is 40-60 kHz.

[0029] The second object of the present invention is to provide a water conditioning composition of bacteria and enzymes prepared by the above preparation method. The amount of the water conditioning composition of bacteria and enzymes added to the water body is 1-10 g / m 3 .

[0030] Beneficial effects of the present invention:

[0031] 1. The present invention uses different photosynthetic bacteria, nitrifying bacteria, composite fungi, and composite enzymes in combination, and prepares a water quality conditioning composition that can be slowly released by emulsification and spray drying. The composition can be applied to different water bodies and effectively reduce the chemical oxygen demand (COD), permanganate index (COD Mn ) and total phosphorus index, and can inhibit the growth of pathogens and harmful algae in water bodies. The metabolites produced by microorganisms have a good adsorption and flocculation effect on heavy metals and colloidal substances in water bodies, which can promote the balance of bacteria and algae in water bodies and is suitable for the ecological restoration of natural water bodies.

[0032] 2. The present invention uses different photosynthetic bacteria in combination and utilizes various metabolic pathways in the body to effectively reduce the concentration of pollutants such as ammonia nitrogen, sulfide, and nitrite in the water body, increase the dissolved oxygen content of the water body, and have a good removal effect on heavy metals. At the same time, after adding these four different photosynthetic bacteria to the water body, algae such as diatoms and chlorella in the water body can be made dominant algae, inhibiting the reproduction of harmful algae such as cyanobacteria. By decomposing harmful organic matter and replenishing the bacterial community, a balance between bacteria and algae is achieved, the water quality is not easily changed, the water quality can be stabilized, and the balance of the water ecosystem can be maintained.

[0033] Bacillus subtilis is an aerobic bacterium that grows fast, has low nutritional requirements, can efficiently secrete a variety of proteins and metabolites, and does not produce toxins. It is a non-pathogenic and safe microorganism. The active substances such as subtilisin, polymyxin, nystatin, and gramicidin produced during the growth of the bacteria have a significant inhibitory effect on pathogenic bacteria in water bodies. Iron-oxidizing bacteria use CO2 as a carbon source and oxidize Fe 2+ to Fe 3+ The autotrophic microorganisms that obtain energy to grow can produce iron hydroxide precipitates with a flocculation effect, which can effectively adsorb colloidal particulate matter in the water, making the water clearer; Nitrosomonas converts ammonia nitrogen into nitrite, and Nitrobacter converts nitrite into nitrate, effectively removing ammonia nitrogen from the water.

[0034] 3. The present invention uses Aspergillus niger spore powder, Trichoderma T22, and brewer's yeast in combination to obtain a composite fungus, wherein the Aspergillus niger spore powder is the reproductive cell of Aspergillus niger, has strong environmental adaptability, can produce microbial flocculants such as extracellular polysaccharides, and its flocculation effect is better than that of traditional chemical flocculants such as polyacrylamide and polyaluminum chloride. It can effectively adsorb heavy metal pollutants in water bodies, and can also produce amylase, acid protease, cellulase, pectinase, glucose oxidase, citric acid, gluconic acid and gallic acid, and further hydrolyze various organic substances such as proteins, cellulose, pectin, etc. in the water body through the catalytic action of the enzyme; Trichoderma T22 can produce small-molecule antibiotics and large-molecule antibacterial proteins or cell wall-degrading enzymes to inhibit the growth, reproduction and infection of pathogens. In antibiotics and fungal parasitism, Trichoderma can produce chitinase, glucanase, cellulase and protease to decompose the cell walls of plant pathogenic fungi or secrete extracellular enzymes such as glucosidase to degrade antibiotic toxins produced by pathogens; brewer's yeast is a heterotrophic facultative anaerobic microorganism that can inhibit the reproduction of pathogens and at the same time produce growth-promoting factors to promote the growth and reproduction of beneficial bacteria.

[0035] 4. The present invention also adds complex enzymes based on microorganisms, among which dispase can decompose organic substances such as starch, protein and fat in water. Humic acid is a type of organic substance accumulated after the remains of animals and plants are decomposed and transformed by microorganisms. The total amount in water is large. Humic acid is decomposed into smaller organic molecules by humic acid enzymes, which can fully promote nutrient circulation and activity. Saccharifying enzymes can hydrolyze starch and dextrin to release glucose. By utilizing the catalytic decomposition effect of complex enzymes, the organic matter content in water can be effectively reduced, thereby reducing the chemical oxygen demand (COD) and permanganate index (COD) in water. Mn ) and total phosphorus index purposes.

[0036] 5. After being modified, the specific surface area of ​​bentonite can be increased, so that its adsorption capacity is enhanced, and it has good suspension performance, so that various microorganisms and complex enzymes in the combined water quality conditioning composition of bacteria and enzymes are evenly dispersed and adsorbed in the pores of the modified bentonite, and then coated with chitosan to form a microcapsule structure. When the combined water quality conditioning composition of bacteria and enzymes is put into water, the modified bentonite can absorb water and expand in volume, so that various microorganisms and complex enzymes coated in the chitosan are slowly released. Chitosan and modified bentonite themselves have excellent adsorption capacity, which can further adsorb pollutants in the water body, and work together with microorganisms and enzymes to improve the water environment.

[0037] 6. The preparation method of the bacterial enzyme combined water conditioning composition of the present invention is simple, the production cost is low, and it can be used for the treatment of polluted water bodies. The dosage is 1-10g / m 3 , which has great promotion value. DETAILED DESCRIPTION

[0038] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below in conjunction with the specific implementation methods of the specification.

[0039] The experimental methods in the following examples are conventional methods unless otherwise specified. The preparations involved in the following examples are common commercial products and can be purchased from the market unless otherwise specified.

[0040] The features and performance of the present invention are further described in detail below with reference to the embodiments.

[0041] Example 1: Preparation of modified bentonite

[0042] S11, mixing bentonite with 5% acetic acid solution at a mass volume ratio (g:mL) of 10:120, ultrasonically treating the mixture at 50 kHz for 30 minutes, centrifuging the mixture at 6000 rpm for 10 minutes, filtering the mixture, washing the precipitate three times with 3 volumes of deionized water, and drying the mixture at 120°C for 1.5 hours to obtain pretreated bentonite;

[0043] S12. The pretreated bentonite obtained in S11, anionic polyacrylamide, aluminate coupling agent, and deionized water were mixed in a mass ratio of 30:8:0.8:120, and then ultrasonically treated at 60 kHz for 15 minutes. The mixture was allowed to stand for 5 hours, filtered, and dried to obtain modified bentonite.

[0044] Example 2: Preparation of modified bentonite

[0045] S11, mixing bentonite with 8% acetic acid solution at a mass volume ratio (g:mL) of 8:120, ultrasonically treating the mixture at 40 kHz for 30 minutes, centrifuging the mixture at 5000 rpm for 10 minutes, filtering the mixture, washing the precipitate three times with 3 volumes of deionized water, and drying the mixture at 100°C for 2 hours to obtain pretreated bentonite;

[0046] S12. The pretreated bentonite obtained in S11, anionic polyacrylamide, aluminate coupling agent, and deionized water were mixed in a mass ratio of 20:5:1:100, subjected to 50 kHz ultrasonic treatment for 20 minutes, allowed to stand for 4 hours, filtered, and dried to obtain modified bentonite.

[0047] Example 3: Preparation of modified bentonite

[0048] S11, mixing bentonite with 5-10% acetic acid solution at a mass volume ratio (g:mL) of 9:100, ultrasonically treating the mixture at 60 kHz for 30 minutes, centrifuging the mixture at 6000 rpm for 8 minutes, filtering the mixture, washing the precipitate three times with 3 volumes of deionized water, and drying the mixture at 110°C for 1 hour to obtain pretreated bentonite;

[0049] S12. The pretreated bentonite obtained in S11, anionic polyacrylamide, aluminate coupling agent, and deionized water were mixed in a mass ratio of 40:10:0.5:150, and then ultrasonically treated at 40 kHz for 15 minutes. The mixture was allowed to stand for 6 hours, filtered, and dried to obtain modified bentonite.

[0050] Example 4: Preparation of a water conditioning composition combining bacteria and enzymes

[0051] S1. Preparation of a composite bacterial agent: Rhodopseudomonas sphaeroides, Rhodobacter sphaeroides, purple non-sulfur photosynthetic bacteria, Rhodobacter capsulatus, Bacillus subtilis, iron-oxidizing bacteria, Nitrosomonas, and Nitrobacter were respectively cultured on separate culture media to obtain the corresponding bacterial liquids, which were then centrifuged at 10,000 rpm to obtain bacterial slurry, a protective agent 4 times the mass of the bacterial slurry was added, and the bacterial powders were freeze-dried to obtain bacterial powders, which were mixed in a ratio of 1:1:1:1:3:2:0.5:1 to obtain a composite bacterial agent;

[0052] The protective agent is a mixture of 3% glycerol, 3% glucose solution and 0.9% sodium chloride solution in a mass ratio of 2:1:0.8;

[0053] S2. Preparation of core material: 80 parts of the composite bacterial agent obtained in S1, 10 parts of Aspergillus niger spore powder, 20 parts of Trichoderma T22, 10 parts of brewer's yeast powder, 3 parts of dispase, 1 part of humase, and 1 part of saccharifying enzyme were dissolved in 500 parts of corn oil, and then 40 parts of the modified bentonite obtained in Example 1 and 2 parts of cetyl polyethylene glycol were added. The mixture was emulsified and dispersed at 10,000 rpm at room temperature for 20 minutes to obtain a core material;

[0054] S3. Preparation of wall material: dissolving chitosan in deionized water to obtain a chitosan solution with a mass volume ratio of 2%, which is the wall material;

[0055] S4. Prepare a bacterial-enzyme combined water conditioning composition: mix the core material obtained in S2 and the wall material obtained in S3 in a mass ratio of 5:1, disperse at 10,000 rpm for 30 minutes, and spray dry to obtain a bacterial-enzyme combined water conditioning composition.

[0056] Example 5: Preparation of a water conditioning composition combining bacteria and enzymes

[0057] S1. Preparation of a composite bacterial agent: Rhodopseudomonas sphaeroides, Rhodobacter sphaeroides, purple non-sulfur photosynthetic bacteria, Rhodobacter capsulatus, Bacillus subtilis, iron-oxidizing bacteria, Nitrosomonas, and Nitrobacter were respectively cultured on separate culture media to obtain the corresponding bacterial liquids, which were then centrifuged at 10,000 rpm to obtain bacterial slurry, a protective agent twice the mass of the bacterial slurry was added, and the mixture was freeze-dried to obtain bacterial powder, which was then mixed in a ratio of 1:1:1:1:2:3:0.5:0.5 to obtain a composite bacterial agent;

[0058] The protective agent is a mixture of 2% glycerol, 4% glucose solution and 0.9% sodium chloride solution in a mass ratio of 3:1:1;

[0059] S2. Preparation of core material: 90 parts of the composite bacterial agent obtained in S1, 10 parts of Aspergillus niger spore powder, 25 parts of Trichoderma T22, 15 parts of brewer's yeast powder, 2 parts of dispase, 1 part of humase, and 1 part of saccharifying enzyme were dissolved in 450 parts of corn oil, and then 45 parts of the modified bentonite obtained in Example 2 and 3 parts of cetyl polyethylene glycol were added. The mixture was emulsified and dispersed at 8000 rpm at room temperature for 25 minutes to obtain the core material;

[0060] S3. Preparation of wall material: dissolving chitosan in deionized water to obtain a chitosan solution with a mass volume ratio of 3%, which is the wall material;

[0061] S4. Prepare a bacterial-enzyme combined water conditioning composition: mix the core material obtained in S2 and the wall material obtained in S3 in a mass ratio of 4:1, disperse at 8000 rpm for 40 minutes, and spray dry to obtain a bacterial-enzyme combined water conditioning composition.

[0062] Example 6: Preparation of a water conditioning composition combining bacteria and enzymes

[0063] S1. Preparation of a composite bacterial agent: Rhodopseudomonas sphaeroides, Rhodobacter sphaeroides, purple non-sulfur photosynthetic bacteria, Rhodobacter capsulatus, Bacillus subtilis, iron-oxidizing bacteria, Nitrosomonas, and Nitrobacter were respectively cultured on separate culture media to obtain the corresponding bacterial liquids, which were then centrifuged at 12,000 rpm to obtain bacterial slurry, a protective agent 3 times the mass of the bacterial slurry was added, and the bacterial powders were freeze-dried to obtain bacterial powders, which were mixed in a ratio of 1:1:1:1:1.5:2:1:0.8 to obtain a composite bacterial agent;

[0064] The protective agent is a mixture of 1% glycerol, 2% glucose solution and 0.7% sodium chloride solution in a mass ratio of 1:1:0.5;

[0065] S2. Preparation of core material: 100 parts of the composite bacterial agent obtained in S1, 10 parts of Aspergillus niger spore powder, 22 parts of Trichoderma T22, 15 parts of brewer's yeast powder, 2.5 parts of dispase, 1 part of humase, and 1 part of saccharifying enzyme were dissolved in 400 parts of corn oil, and then 50 parts of the modified bentonite obtained in Example 3 and 2 parts of cetyl polyethylene glycol were added. The mixture was emulsified and dispersed at 8000 rpm at room temperature for 20 minutes to obtain the core material;

[0066] S3. Preparation of wall material: dissolving chitosan in deionized water to obtain a chitosan solution with a mass volume ratio of 3%, which is the wall material;

[0067] S4. Prepare a bacterial-enzyme combined water conditioning composition: mix the core material obtained in S2 and the wall material obtained in S3 in a mass ratio of 6:1, disperse at 8000 rpm for 30 minutes, and spray dry to obtain a bacterial-enzyme combined water conditioning composition.

[0068] Comparative Example 1:

[0069] On the basis of Example 4, no composite bacterial agent was added, and other aspects were the same as Example 4.

[0070] Comparative Example 2:

[0071] On the basis of Example 4, no Aspergillus niger spore powder, Trichoderma T22 and brewer's yeast powder were added, and the rest was the same as Example 4.

[0072] Comparative Example 3:

[0073] On the basis of Example 4, no dispase, humase and saccharifying enzyme were added, and the rest was the same as Example 4.

[0074] Comparative Example 4:

[0075] On the basis of Example 4, no modified bentonite was added, and the rest was the same as Example 4.

[0076] Comparative Example 5:

[0077] S1. Preparation of a composite bacterial agent: Rhodopseudomonas sphaeroides, Rhodobacter sphaeroides, purple non-sulfur photosynthetic bacteria, Rhodobacter capsulatus, Bacillus subtilis, iron-oxidizing bacteria, Nitrosomonas, and Nitrobacter were respectively cultured on separate culture media to obtain the corresponding bacterial liquids, which were then centrifuged at 10,000 rpm to obtain bacterial slurry, a protective agent 4 times the mass of the bacterial slurry was added, and the bacterial powders were freeze-dried to obtain bacterial powders, which were mixed in a ratio of 1:1:1:1:3:2:0.5:1 to obtain a composite bacterial agent;

[0078] The protective agent is a mixture of 3% glycerol, 3% glucose solution and 0.9% sodium chloride solution in a mass ratio of 2:1:0.8;

[0079] S2. Preparation of core material: 80 parts of the composite bacterial agent obtained in S1, 3 parts of Aspergillus niger spore powder, 25 parts of Trichoderma T22, 12 parts of brewer's yeast powder, 3 parts of dispase, 1 part of humase, and 1 part of saccharifying enzyme were dissolved in 500 parts of corn oil, and then 40 parts of the modified bentonite obtained in Example 1 and 2 parts of cetyl polyethylene glycol were added. The mixture was emulsified and dispersed at 10,000 rpm at room temperature for 20 minutes to obtain a core material;

[0080] S3. Preparation of wall material: dissolving chitosan in deionized water to obtain a chitosan solution with a mass volume ratio of 2%, which is the wall material;

[0081] S4. Prepare a bacterial-enzyme combined water conditioning composition: mix the core material obtained in S2 and the wall material obtained in S3 in a mass ratio of 5:1, disperse at 10,000 rpm for 30 minutes, and spray dry to obtain a bacterial-enzyme combined water conditioning composition.

[0082] Comparative Example 6:

[0083] S1. Preparation of a composite bacterial agent: Rhodopseudomonas sphaeroides, Rhodobacter sphaeroides, purple non-sulfur photosynthetic bacteria, Rhodobacter capsulatus, Bacillus subtilis, iron-oxidizing bacteria, Nitrosomonas, and Nitrobacter were respectively cultured on separate culture media to obtain the corresponding bacterial liquids, which were then centrifuged at 10,000 rpm to obtain bacterial slurry, a protective agent 4 times the mass of the bacterial slurry was added, and the bacterial powders were freeze-dried to obtain bacterial powders, which were mixed in a ratio of 1:1:1:1:3:2:0.5:1 to obtain a composite bacterial agent;

[0084] The protective agent is a mixture of 3% glycerol, 3% glucose solution and 0.9% sodium chloride solution in a mass ratio of 2:1:0.8;

[0085] S2. Preparation of core material: 80 parts of the composite bacterial agent obtained in S1, 30 parts of Aspergillus niger spore powder, 5 parts of Trichoderma T22, 5 parts of brewer's yeast powder, 3 parts of dispase, 1 part of humase, and 1 part of saccharifying enzyme were dissolved in 500 parts of corn oil, and then 40 parts of the modified bentonite obtained in Example 1 and 2 parts of cetyl polyethylene glycol were added. The mixture was emulsified and dispersed at 10,000 rpm at room temperature for 20 minutes to obtain a core material;

[0086] S3. Preparation of wall material: dissolving chitosan in deionized water to obtain a chitosan solution with a mass volume ratio of 2%, which is the wall material;

[0087] S4. Prepare a bacterial-enzyme combined water conditioning composition: mix the core material obtained in S2 and the wall material obtained in S3 in a mass ratio of 5:1, disperse at 10,000 rpm for 30 minutes, and spray dry to obtain a bacterial-enzyme combined water conditioning composition.

[0088] A 200-300m long ditch was selected in the urban-rural junction of Pingxiang, Jiangxi Province, and divided into 9 sections. 3 The water quality conditioning compositions prepared by the bacterial enzyme combination of Examples 4-6 and Comparative Examples 1-6 were added in the amounts of 100 mg / L and 100 mg / L, respectively. The COD, COD Mn And total phosphorus content is tested, according to the COD, COD Mn and total phosphorus content, and calculate COD, COD Mn The removal rates of the total phosphorus content are shown in Table 1.

[0089] The COD detection method refers to HJ 282-2017 "Water Quality Determination of Chemical Oxygen Demand - Dichromate Method", COD MnThe detection method for total phosphorus content refers to GB / T 11892-1989 "Water quality - Determination of permanganate index and total phosphorus content", and the detection method for total phosphorus content refers to GB / T 11893-1989 "Water quality - Determination of total phosphorus - Ammonium molybdate spectrophotometric method".

[0090] Table 1 Results of water pollutant removal rate

[0091]

[0092] As can be seen from Table 1, after the water quality conditioning composition of the present invention was added in Examples 4-6, the pollutant removal rate showed an upward trend. In the first 7 days, COD, COD Mn The removal rates of COD, COD Mn The removal rate of total phosphorus exceeded 67.15%. On the 20th day, the removal rate of COD was between 97.51% and 98.75%. Mn The removal rate of COD and COD is between 95.42% and 96.78%, and the removal rate of total phosphorus is between 92.38% and 93.27%. Mn , and total phosphorus have better removal effects, and are significantly better than comparative examples 1-6.

[0093] Finally, it should be noted that the above examples are merely specific embodiments of the present invention. Obviously, the present invention is not limited to the above examples and is subject to numerous variations. All variations that can be directly derived or conceived by a person of ordinary skill in the art from the disclosure of the present invention are considered to be within the scope of protection of the present invention.

Claims

1. A method for preparing a water conditioning composition combined with bacteria and enzymes, characterized in that: The steps include: S1. Preparation of a composite bacterial agent: respectively culture Rhodopseudomonas sphaeroides, Rhodobacter sphaeroides, purple non-sulfur photosynthetic bacteria, Rhodobacter capsulatus, Bacillus subtilis, iron-oxidizing bacteria, Nitrosomonas, and Nitrobacter on separate culture media to obtain corresponding bacterial liquids, centrifuge at 10,000-12,000 rpm to obtain bacterial slurry, add a protective agent, and freeze-dry to obtain bacterial powder, and mix the bacterial powders in a ratio of 1:1:1:1:1-3:1-3:0.5-1:0.5-1 to obtain a composite bacterial agent; S2. Preparation of core material: Dissolve 80-100 parts of the composite bacterial agent obtained in S1, 20-50 parts of the composite fungal agent, and 1-5 parts of the composite enzyme in 400-500 parts of corn oil, then add 30-50 parts of modified bentonite and 1-3 parts of cetyl polyethylene glycol, and emulsify and disperse at 8000-10000 rpm at room temperature for 20-30 minutes to obtain the core material; S3. Prepare wall material: dissolve chitosan in deionized water to obtain a chitosan solution with a mass volume ratio of 1% to 3%, which is the wall material; S4. Prepare a bacterial-enzyme combined water conditioning composition: mix the core material obtained in S2 with the wall material obtained in S3 at a mass ratio of 4-6:1, disperse at 8000-10000 rpm for 30-40 minutes, and spray dry to obtain a bacterial-enzyme combined water conditioning composition; The preparation method of the modified bentonite is: S11, after mixing bentonite with 5-10% acetic acid solution by mass, ultrasonically treat for 30-40 minutes, centrifuge at 5000-6000 rpm for 8-10 minutes, filter, wash the precipitate with deionized water 3-4 times, and dry at 100-120° C. for 1-2 hours to obtain pretreated bentonite; S12. Mix the pretreated bentonite obtained in S11, anionic polyacrylamide, aluminate coupling agent, and deionized water in a mass ratio of 20-40:5-10:0.5-1:100-150, perform ultrasonic treatment for 15-20 minutes, let stand for 4-6 hours, filter, and dry to obtain modified bentonite.

2. The method for preparing a water conditioning composition combined with bacteria and enzymes according to claim 1, characterized in that: In S1, the protective agent is a mixture of 1-3% by mass glycerol, 2-4% by mass glucose solution and 0.7-0.9% by mass sodium chloride solution in a mass ratio of 1-3:1:0.5-1.

3. The method for preparing a water conditioning composition combined with bacteria and enzymes according to claim 1, characterized in that: In S1, the amount of the protective agent used is 2-4 times the mass of the bacterial sludge.

4. The method for preparing a water conditioning composition combined with bacteria and enzymes according to claim 1, characterized in that: In S2, the composite fungal agent is a mixture of Aspergillus niger spore powder, Trichoderma T22, and brewer's yeast powder; wherein the mass ratio of Aspergillus niger spore powder, Trichoderma T22, and brewer's yeast powder is 0.5-1:1-2:

1.

5. The method for preparing a water conditioning composition combined with bacteria and enzymes according to claim 1, characterized in that: In S2, the complex enzyme is a mixture of dispase, humase, and saccharifying enzyme; wherein the mass ratio of dispase, humase, and saccharifying enzyme is 2-3:1:

1.

6. The method for preparing a bacterial-enzyme combined water conditioning composition according to claim 1, characterized in that: In S11, the mass volume ratio (g:mL) of the bentonite to the acetic acid solution is 8-10:100-120.

7. The method for preparing a water conditioning composition combined with bacteria and enzymes according to claim 1, characterized in that: In S11, the frequency of the ultrasonic treatment is 40-60 kHz.

8. A water conditioning composition obtained by the bacterial-enzyme combination method according to any one of claims 1 to 7.

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