A method for extracting olefinic substances from oil camphor tree

Abstract

The application discloses a method for extracting olefin substances from Cinnamomum oleiferum. The extraction method comprises the steps of beating, enzymolysis, distillation, static stratification, silica gel column separation and the like. The mixed salt is added in the slurry to adjust the osmotic pressure of the fabric cell, which is beneficial to the damage of the cell wall and the exudation of the effective components, and the mixed salt can promote the activity of the mixed enzyme, so that the mixed enzyme can have better enzymolysis capacity. The mixed enzyme is used for enzymolysis of the plant cell wall, so that the effective components in the plant cell wall are released, and the yield of the Cinnamomum oleiferum oil is improved. The silica gel column is used for separating the Cinnamomum oleiferum essential oil. The olefin substances can be effectively separated from the essential oil due to the difference in polarity between the olefins and the alcohols, esters and the like, so that the olefin substances composed of the olefins are obtained.

Description

Technical Field

[0001] This invention belongs to the field of component extraction technology, specifically relating to a method for extracting olefinic substances from camphor trees. Background Technology

[0002] Cinnamomum camphora is an evergreen tree belonging to the genus Cinnamomum in the family Lauraceae. It is endemic to China, mainly produced in Sichuan Province, and naturally distributed in evergreen broad-leaved forests at altitudes of 600-2000m. Cinnamomum camphora oil is a volatile plant oil extracted from the branches and leaves of the tree, with leaf extraction being the primary method of production. Cinnamomum camphora oil contains over 100 components, with 1,8-cineole accounting for approximately 60%. This substance has important antibacterial and antiviral effects, especially showing strong preventative effects against coronaviruses. In addition, Cinnamomum camphora oil contains a significant amount of alkenes, which have important applications in fragrances and pharmaceuticals. However, there are few reports in current technology of obtaining alkenes from Cinnamomum camphora. Summary of the Invention

[0003] In view of the above-mentioned prior art, the present invention provides a method for extracting olefins from camphor trees.

[0004] To achieve the above objectives, the technical solution adopted by the present invention is to provide a method for extracting alkene substances from camphor trees, comprising the following steps:

[0005] S1: Stir and crush fresh camphor leaves into a pulp, and mix the pulp with water at a volume ratio of 1:1 to 3 to obtain a slurry;

[0006] S2: Add mixed enzymes and mixed salts to the slurry, mix well, and then enzymatically hydrolyze at 20-30℃ for 24-48 hours to obtain the enzymatic hydrolysate; the mixed enzymes include cellulase, hemicellulase and amylase, and the mixed salts include calcium salts and zinc salts.

[0007] S3: Distill the enzymatically hydrolyzed material to obtain camphor oil;

[0008] S4: Mix camphor oil and propylene glycol solution at a volume ratio of 1:1 to 3, shake vigorously for 3 to 5 minutes, then let stand for 0.5 to 1 hour, collect the upper oil layer to obtain camphor essential oil;

[0009] S5: Mix camphor essential oil with anhydrous ethanol at a mass ratio of 1:2 to 5, then mix the mixture with silica gel with a particle size of 37 to 54 μm at a mass ratio of 1:1 to 3 to obtain a separated sample; pack the silica gel with a particle size of 37 to 54 μm into a chromatography column with an inner diameter of 50 mm and a length of 500 mm to obtain a separation column;

[0010] S6: Place the sample to be separated at the top of the separation column, with the mass ratio of the sample to the silica gel in the column being 1:15-20; then elute with a mixed eluent obtained by mixing diethyl ether and n-hexane at a volume ratio of 5-10:90-95. Start timing when the eluent flows out from the bottom of the separation column, and start collecting the eluent after two minutes. Stop collecting after 40-45 minutes.

[0011] S7: Concentrate the eluent collected in S6 to obtain the final product.

[0012] Based on the above technical solution, the present invention can be further improved as follows.

[0013] Furthermore, the mass ratio of the mixed enzyme, mixed salt and slurry is 0.1-0.5:0.01-0.2:90-100.

[0014] Furthermore, the mass ratio of the mixed enzyme, mixed salt, and slurry is 0.2:0.1:100.

[0015] Furthermore, the mixed enzyme is composed of cellulase, hemicellulase and amylase mixed in a mass ratio of 5-10:3-5:1-3.

[0016] Furthermore, the mixed salt is made by mixing calcium salt and zinc salt in a mass ratio of 3 to 5: 1 to 3.

[0017] Furthermore, the calcium salt is calcium chloride, calcium nitrate, or calcium sulfate; the zinc salt is zinc chloride, zinc sulfate, or zinc nitrate.

[0018] Furthermore, the propylene glycol solution is prepared by mixing propylene glycol and water in a volume ratio of 1:3 to 5.

[0019] Furthermore, in S5, camphor oil and anhydrous ethanol are mixed at a mass ratio of 1:3, and the resulting mixture is then mixed with silica gel at a mass ratio of 1:2.

[0020] Furthermore, the volume ratio of diethyl ether to n-hexane in the mixed eluent is 7:93.

[0021] The beneficial effects of this invention are:

[0022] 1. This application involves mechanically agitating fresh camphor leaves to form a slurry. During this process, the camphor sap cells are mechanically broken down to a certain extent, which facilitates the release of active ingredients. Subsequently, a mixed enzyme and a mixed salt are added. The addition of the mixed salt regulates the osmotic pressure of the plant cells, promoting cell wall disruption and the release of active ingredients. Furthermore, the mixed salt enhances the activity of the mixed enzyme, allowing it to exert better enzymatic hydrolysis capabilities. The mixed enzyme hydrolyzes the plant cell walls, releasing the active ingredients and thus increasing the yield of camphor oil.

[0023] 2. After obtaining camphor oil, mix it with propylene glycol solution. The alcohols and esters in camphor oil are soluble in propylene glycol and water. By shaking and letting it stand, some of the alcohols and esters in camphor oil can be removed, which is beneficial for the separation and purification of alkenes.

[0024] 3. This application uses a silica gel column to separate camphor essential oil. By taking advantage of the difference in polarity between alkenes and alcohols, esters, etc., alkenes in the essential oil can be effectively separated, thereby obtaining alkenes that are basically composed entirely of alkenes. Detailed Implementation

[0025] The specific embodiments of the present invention will be described in detail below with reference to examples.

[0026] Example 1

[0027] A method for extracting alkenes from camphor trees includes the following steps:

[0028] S1: Use a pulping machine to stir and crush the washed fresh camphor leaves into pulp, and mix the pulp with water at a volume ratio of 1:2 to obtain slurry;

[0029] S2: Add mixed enzymes and mixed salts to the slurry, mix well, and then enzymatically hydrolyze at 25℃ for 36 hours to obtain the enzymatic hydrolysate; wherein, the mixed enzymes are composed of cellulase, hemicellulase and amylase in a mass ratio of 8:4:2, and the mixed salts are composed of calcium chloride and zinc chloride in a mass ratio of 4:2; the mass ratio of mixed enzymes, mixed salts to slurry is 0.2:0.1:100;

[0030] S3: Distill the enzymatic hydrolysate using an existing steam distillation apparatus to obtain camphor oil;

[0031] S4: Mix camphor oil and propylene glycol solution at a volume ratio of 1:2, shake vigorously for 4 minutes, then let stand for 45 minutes, collect the upper oil layer to obtain camphor essential oil; wherein, the propylene glycol solution is prepared by mixing propylene glycol and water at a volume ratio of 1:4.

[0032] S5: Mix camphor essential oil and anhydrous ethanol at a mass ratio of 1:4, then mix the mixture with silica gel with a particle size of 37-54 μm (300-400 mesh) at a mass ratio of 1:2 to obtain a separated sample; pack the silica gel with a particle size of 37-54 μm into a chromatography column with an inner diameter of 50 mm and a length of 500 mm to obtain a separation column;

[0033] S6: Place the sample to be separated at the top of the separation column, with the mass ratio of the sample to the silica gel in the column being 1:15; then elute with a mixed eluent obtained by mixing diethyl ether and n-hexane at a volume ratio of 7:93. Start timing when the eluent flows out from the bottom of the separation column, and start collecting the eluent after two minutes and stop collecting after 45 minutes.

[0034] S7: Concentrate the eluent collected in S6 to obtain the final product.

[0035] Example 2

[0036] A method for extracting alkenes from camphor trees includes the following steps:

[0037] S1: Use a pulping machine to stir and crush the washed fresh camphor leaves into pulp, and mix the pulp with water at a volume ratio of 1:2 to obtain slurry;

[0038] S2: Add mixed enzymes and mixed salts to the slurry, mix well, and then enzymatically hydrolyze at 25℃ for 36 hours to obtain the enzymatic hydrolysate; wherein, the mixed enzymes are composed of cellulase, hemicellulase and amylase in a mass ratio of 5:3:1, and the mixed salts are composed of calcium nitrate and zinc nitrate in a mass ratio of 3:1; the mass ratio of mixed enzymes, mixed salts to slurry is 0.1:0.1:100;

[0039] S3: Distill the enzymatic hydrolysate using an existing steam distillation apparatus to obtain camphor oil;

[0040] S4: Mix camphor oil and propylene glycol solution at a volume ratio of 1:2, shake vigorously for 4 minutes, then let stand for 45 minutes, collect the upper oil layer to obtain camphor essential oil; wherein, the propylene glycol solution is prepared by mixing propylene glycol and water at a volume ratio of 1:4.

[0041] S5: Mix camphor essential oil and anhydrous ethanol at a mass ratio of 1:4, then mix the mixture with silica gel with a particle size of 37-54 μm (300-400 mesh) at a mass ratio of 1:2 to obtain a separated sample; pack the silica gel with a particle size of 37-54 μm into a chromatography column with an inner diameter of 50 mm and a length of 500 mm to obtain a separation column;

[0042] S6: Place the sample to be separated at the top of the separation column, with the mass ratio of the sample to the silica gel in the column being 1:15; then elute with a mixed eluent obtained by mixing diethyl ether and n-hexane at a volume ratio of 7:93. Start timing when the eluent flows out from the bottom of the separation column, and start collecting the eluent after two minutes and stop collecting after 45 minutes.

[0043] S7: Concentrate the eluent collected in S6 to obtain the final product.

[0044] Example 3

[0045] A method for extracting alkenes from camphor trees includes the following steps:

[0046] S1: Use a pulping machine to stir and crush the washed fresh camphor leaves into pulp, and mix the pulp with water at a volume ratio of 1:2 to obtain slurry;

[0047] S2: Add mixed enzymes and mixed salts to the slurry, mix well, and then enzymatically hydrolyze at 25℃ for 36 hours to obtain the enzymatic hydrolysate; wherein, the mixed enzyme is composed of cellulase, hemicellulase and amylase in a mass ratio of 10:5:3, and the mixed salt is composed of calcium sulfate and zinc sulfate in a mass ratio of 5:3; the mass ratio of mixed enzymes, mixed salts to slurry is 0.5:0.01:90;

[0048] S3: Distill the enzymatic hydrolysate using an existing steam distillation apparatus to obtain camphor oil;

[0049] S4: Mix camphor oil and propylene glycol solution at a volume ratio of 1:2, shake vigorously for 4 minutes, then let stand for 45 minutes, collect the upper oil layer to obtain camphor essential oil; wherein, the propylene glycol solution is prepared by mixing propylene glycol and water at a volume ratio of 1:4.

[0050] S5: Mix camphor essential oil and anhydrous ethanol at a mass ratio of 1:4, then mix the mixture with silica gel with a particle size of 37-54 μm (300-400 mesh) at a mass ratio of 1:2 to obtain a separated sample; pack the silica gel with a particle size of 37-54 μm into a chromatography column with an inner diameter of 50 mm and a length of 500 mm to obtain a separation column;

[0051] S6: Place the sample to be separated at the top of the separation column, with the mass ratio of the sample to the silica gel in the column being 1:15; then elute with a mixed eluent obtained by mixing diethyl ether and n-hexane at a volume ratio of 7:93. Start timing when the eluent flows out from the bottom of the separation column, and start collecting the eluent after two minutes and stop collecting after 45 minutes.

[0052] S7: Concentrate the eluent collected in S6 to obtain the final product.

[0053] Example 4

[0054] A method for extracting alkenes from camphor trees includes the following steps:

[0055] S1: Use a pulping machine to stir and crush the washed fresh camphor leaves into pulp, and mix the pulp with water at a volume ratio of 1:1 to obtain slurry;

[0056] S2: Add mixed enzymes and mixed salts to the slurry, mix well, and then enzymatically hydrolyze at 30℃ for 24 hours to obtain the enzymatic hydrolysate; wherein, the mixed enzymes are composed of cellulase, hemicellulase and amylase in a mass ratio of 8:4:2, and the mixed salts are composed of calcium chloride and zinc chloride in a mass ratio of 4:2; the mass ratio of mixed enzymes, mixed salts to slurry is 0.1:0.01:90;

[0057] S3: Distill the enzymatic hydrolysate using an existing steam distillation apparatus to obtain camphor oil;

[0058] S4: Mix camphor oil and propylene glycol solution at a volume ratio of 1:1, shake vigorously for 5 minutes, then let stand for 60 minutes, collect the upper oil layer to obtain camphor essential oil; wherein, the propylene glycol solution is prepared by mixing propylene glycol and water at a volume ratio of 1:3.

[0059] S5: Mix camphor essential oil and anhydrous ethanol at a mass ratio of 1:2, then mix the mixture with silica gel with a particle size of 37-54 μm (300-400 mesh) at a mass ratio of 1:1 to obtain a separated sample; pack the silica gel with a particle size of 37-54 μm into a chromatography column with an inner diameter of 50 mm and a length of 500 mm to obtain a separation column;

[0060] S6: Place the sample to be separated at the top of the separation column, with the mass ratio of the sample to the silica gel in the column being 1:20; then elute with a mixed eluent obtained by mixing diethyl ether and n-hexane at a volume ratio of 5:95. The timing starts when the eluent flows out from the bottom of the separation column. Start collecting the eluent after two minutes and stop collecting after 40 minutes.

[0061] S7: Concentrate the eluent collected in S6 to obtain the final product.

[0062] Example 5

[0063] A method for extracting alkenes from camphor trees includes the following steps:

[0064] S1: Use a pulping machine to stir and crush the washed fresh camphor leaves into pulp, and mix the pulp with water at a volume ratio of 1:3 to obtain slurry;

[0065] S2: Add mixed enzymes and mixed salts to the slurry, mix well, and then enzymatically hydrolyze at 20℃ for 48 hours to obtain the enzymatic hydrolysate; wherein, the mixed enzymes are composed of cellulase, hemicellulase and amylase in a mass ratio of 8:4:2, and the mixed salts are composed of calcium chloride and zinc chloride in a mass ratio of 4:2; the mass ratio of mixed enzymes, mixed salts to slurry is 0.2:0.1:100;

[0066] S3: Distill the enzymatic hydrolysate using an existing steam distillation apparatus to obtain camphor oil;

[0067] S4: Mix camphor oil and propylene glycol solution at a volume ratio of 1:3, shake vigorously for 3 minutes, then let stand for 30 minutes, collect the upper oil layer to obtain camphor essential oil; wherein, the propylene glycol solution is prepared by mixing propylene glycol and water at a volume ratio of 1:5.

[0068] S5: Mix camphor essential oil and anhydrous ethanol at a mass ratio of 1:5, then mix the mixture with silica gel with a particle size of 37-54 μm (300-400 mesh) at a mass ratio of 1:3 to obtain a separated sample; pack the silica gel with a particle size of 37-54 μm into a chromatography column with an inner diameter of 50 mm and a length of 500 mm to obtain a separation column;

[0069] S6: Place the sample to be separated at the top of the separation column, with the mass ratio of the sample to the silica gel in the column being 1:15; then elute with a mixed eluent obtained by mixing diethyl ether and n-hexane at a volume ratio of 10:90. The timing starts when the eluent flows out from the bottom of the separation column. Start collecting the eluent after two minutes and stop collecting after 45 minutes.

[0070] S7: Concentrate the eluent collected in S6 to obtain the final product.

[0071] Comparative Example 1

[0072] A method for extracting alkenes from camphor trees includes the following steps:

[0073] S1: Use a pulping machine to stir and crush the washed fresh camphor leaves into pulp, and mix the pulp with water at a volume ratio of 1:2 to obtain slurry;

[0074] S2: Add mixed enzymes to the slurry, mix well, and then enzymatically hydrolyze at 25℃ for 36 hours to obtain enzymatic hydrolysate; wherein, the mixed enzymes are composed of cellulase, hemicellulase and amylase in a mass ratio of 8:4:2; the mass ratio of mixed enzymes to slurry is 0.2:100;

[0075] S3: Distill the enzymatic hydrolysate using an existing steam distillation apparatus to obtain camphor oil;

[0076] S4: Mix camphor oil and propylene glycol solution at a volume ratio of 1:2, shake vigorously for 4 minutes, then let stand for 45 minutes, collect the upper oil layer to obtain camphor essential oil; wherein, the propylene glycol solution is prepared by mixing propylene glycol and water at a volume ratio of 1:4.

[0077] S5: Mix camphor essential oil and anhydrous ethanol at a mass ratio of 1:4, then mix the mixture with silica gel with a particle size of 37-54 μm (300-400 mesh) at a mass ratio of 1:2 to obtain a separated sample; pack the silica gel with a particle size of 37-54 μm into a chromatography column with an inner diameter of 50 mm and a length of 500 mm to obtain a separation column;

[0078] S6: Place the sample to be separated at the top of the separation column, with the mass ratio of the sample to the silica gel in the column being 1:15; then elute with a mixed eluent obtained by mixing diethyl ether and n-hexane at a volume ratio of 7:93. Start timing when the eluent flows out from the bottom of the separation column, and start collecting the eluent after two minutes and stop collecting after 45 minutes.

[0079] S7: Concentrate the eluent collected in S6 to obtain the final product.

[0080] Comparative Example 2

[0081] A method for extracting alkenes from camphor trees includes the following steps:

[0082] S1: Use a pulping machine to stir and crush the washed fresh camphor leaves into pulp, and mix the pulp with water at a volume ratio of 1:2 to obtain slurry;

[0083] S2: Add mixed enzymes and calcium chloride to the slurry, mix well, and then enzymatically hydrolyze at 25°C for 36 hours to obtain enzymatic hydrolysate; wherein, the mixed enzyme is composed of cellulase, hemicellulase and amylase mixed in a mass ratio of 8:4:2; the mass ratio of mixed enzymes, calcium chloride and slurry is 0.2:0.1:100;

[0084] S3: Distill the enzymatic hydrolysate using an existing steam distillation apparatus to obtain camphor oil;

[0085] S4: Mix camphor oil and propylene glycol solution at a volume ratio of 1:2, shake vigorously for 4 minutes, then let stand for 45 minutes, collect the upper oil layer to obtain camphor essential oil; wherein, the propylene glycol solution is prepared by mixing propylene glycol and water at a volume ratio of 1:4.

[0086] S5: Mix camphor essential oil and anhydrous ethanol at a mass ratio of 1:4, then mix the mixture with silica gel with a particle size of 37-54 μm (300-400 mesh) at a mass ratio of 1:2 to obtain a separated sample; pack the silica gel with a particle size of 37-54 μm into a chromatography column with an inner diameter of 50 mm and a length of 500 mm to obtain a separation column;

[0087] S6: Place the sample to be separated at the top of the separation column, with the mass ratio of the sample to the silica gel in the column being 1:15; then elute with a mixed eluent obtained by mixing diethyl ether and n-hexane at a volume ratio of 7:93. Start timing when the eluent flows out from the bottom of the separation column, and start collecting the eluent after two minutes and stop collecting after 45 minutes.

[0088] S7: Concentrate the eluent collected in S6 to obtain the final product.

[0089] Comparative Example 3

[0090] A method for extracting alkenes from camphor trees includes the following steps:

[0091] S1: Use a pulping machine to stir and crush the washed fresh camphor leaves into pulp, and mix the pulp with water at a volume ratio of 1:2 to obtain slurry;

[0092] S2: Distill the slurry using existing steam distillation equipment to obtain camphor oil;

[0093] S3: Mix camphor oil and propylene glycol solution at a volume ratio of 1:2, shake vigorously for 4 minutes, then let stand for 45 minutes, collect the upper oil layer to obtain camphor essential oil; wherein, the propylene glycol solution is prepared by mixing propylene glycol and water at a volume ratio of 1:4.

[0094] S4: Mix camphor essential oil and anhydrous ethanol at a mass ratio of 1:4, then mix the mixture with silica gel with a particle size of 37-54 μm (300-400 mesh) at a mass ratio of 1:2 to obtain a separated sample; pack the silica gel with a particle size of 37-54 μm into a chromatography column with an inner diameter of 50 mm and a length of 500 mm to obtain a separation column;

[0095] S5: Place the sample to be separated at the top of the separation column, with the mass ratio of the sample to the silica gel in the column being 1:15; then elute with a mixed eluent obtained by mixing diethyl ether and n-hexane at a volume ratio of 7:93. Start timing when the eluent flows out from the bottom of the separation column, and start collecting the eluent after two minutes and stop collecting after 45 minutes.

[0096] S6: Concentrate the eluent collected in S5 to obtain the final product.

[0097] Results Analysis

[0098] The yields of camphor oil and alkenes in each example and comparative example were calculated using the following formulas:

[0099]

[0100]

[0101] Wherein, m(camphor essential oil), m(alkene), and m(camphor leaf) represent the mass of camphor essential oil, alkene, and camphor leaf, respectively. The results are shown in Table 1.

[0102] Table 1. Yields of camphor oil and alkenes

[0103]

[0104]

[0105] As can be seen from Table 1, the extraction process described in this application yields a high yield of camphor oil, which in turn results in a high yield of olefins.

[0106] Compared with Example 1, Comparative Example 1 did not add mixed salt during the enzymatic hydrolysis of the slurry. This could not regulate the osmotic pressure of the leaf cells in the slurry, nor could it promote the activity of the complex enzyme, resulting in poor enzymatic hydrolysis. The effective components in the leaves could not be effectively exuded, which in turn affected the yield of camphor oil and alkenes.

[0107] Compared with Example 1, Comparative Example 2 only added calcium chloride and mixed enzymes during slurry enzymatic hydrolysis, without adding zinc salt. Although the osmotic pressure could be adjusted, the promotion of enzyme activity was limited, resulting in poor enzymatic hydrolysis and consequently affecting the yield of camphor oil and alkenes.

[0108] Compared with Example 1, Comparative Example 3 did not undergo enzymatic hydrolysis. Simple steam distillation alone could not effectively distill out the effective components in the slurry, which seriously affected the yield of camphor oil and alkenes.

[0109] The alkenes obtained were analyzed according to the method described in GB / T 11538-2006. The alkenes were found to mainly include β-phellandrene, limonene, γ-terpinene, 4-carene, (+)-limonene oxide, α-pinene, galangene, aristolochine, β-boronene, β-elemene, (+)-ausnerene, longifolene, (Z)-caryophyllene, β-caryophyllene, eucalyptolene, (-)-α-piperazine, α-caryophyllene, eucalyptolene, β-almondene, hydrated juniperene, β-serlingene, α-guaiacolene, juniperene, and caryophyllene oxide, etc. The total content of alkenes accounted for more than 98% of the alkenes.

[0110] Although specific embodiments of the present invention have been described in detail with reference to examples, they should not be construed as limiting the scope of protection of this patent. Various modifications and variations that can be made by those skilled in the art without inventive effort within the scope described in the claims are still within the scope of protection of this patent.

Claims

1. A method for extracting alkene compounds from camphor trees, characterized in that, Includes the following steps: S1: Stir and crush fresh camphor leaves into a pulp, and mix the pulp with water at a volume ratio of 1:1~3 to obtain a slurry; S2: Add a mixed enzyme and a mixed salt to the slurry, mix well, and then enzymatically hydrolyze at 20-30℃ for 24-48 hours to obtain the hydrolysate; the mixed enzyme includes cellulase, hemicellulase, and amylase, and the mixed salt includes calcium salt and zinc salt, wherein the calcium salt is calcium chloride, calcium nitrate, or calcium sulfate, and the zinc salt is zinc chloride, zinc sulfate, or zinc nitrate; the mass ratio of the mixed enzyme, mixed salt, and slurry is 0.1-0.5:0.01-0.2:90-100; the mixed enzyme is composed of cellulase, hemicellulase, and amylase mixed in a mass ratio of 5-10:3-5:1-3; the mixed salt is composed of calcium salt and zinc salt mixed in a mass ratio of 3-5:1-3. S3: Distill the enzymatically hydrolyzed material to obtain camphor oil; S4: Mix camphor oil and propylene glycol solution at a volume ratio of 1:1~3, shake vigorously for 3~5 minutes, then let stand for 0.5~1 hours, collect the upper oil layer to obtain camphor essential oil; S5: Mix camphor essential oil with anhydrous ethanol at a mass ratio of 1:2~5, then mix the mixture with silica gel with a particle size of 37~54μm at a mass ratio of 1:1~3 to obtain a separated sample; pack the silica gel with a particle size of 37~54μm into a chromatography column with an inner diameter of 50mm and a length of 500mm to obtain a separation column; S6: Place the sample to be separated at the top of the separation column, with the mass ratio of the sample to the silica gel in the column being 1:15~20; then elute with a mixed eluent obtained by mixing diethyl ether and n-hexane at a volume ratio of 5~10:90~95. Start timing when the eluent flows out from the bottom of the separation column, and start collecting the eluent after two minutes. Stop collecting after 40~45 minutes. S7: Concentrate the eluent collected in S6 to obtain the final product.

2. The method for extracting alkenes from camphor trees according to claim 1, characterized in that: The mass ratio of the mixed enzyme, mixed salt and slurry is 0.2:0.1:

100.

3. The method for extracting alkenes from camphor trees according to claim 1, characterized in that: The propylene glycol solution is prepared by mixing propylene glycol and water in a volume ratio of 1:3 to 5.

4. The method for extracting alkenes from camphor trees according to claim 1, characterized in that: S5 camphor essential oil and anhydrous ethanol are mixed at a mass ratio of 1:3, and the resulting mixture is then mixed with silica gel at a mass ratio of 1:

2.

5. The method for extracting alkenes from camphor trees according to claim 1, characterized in that: The volume ratio of diethyl ether to n-hexane in the mixed eluent is 7:93.

Images