Composition with anti-aging effect and application thereof

Abstract

The present invention relates to a composition with anti-aging efficacy and its use. The composition with anti-aging efficacy comprises a Bidens pilosa extract and a Pansy tricolor extract. The present invention combines the Bidens pilosa extract and the Pansy tricolor extract to exert anti-aging efficacy through different mechanistic pathways. The two extracts exhibit significant synergistic effects in terms of anti-aging efficacy, specifically demonstrating significant skin elasticity enhancement, anti-oxidation, and anti-wrinkle effects. The composition is highly suitable for use in skin care products, is mild and non-irritating, and exhibits excellent stability.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and relates to a composition with anti-aging effect and an application thereof, and particularly to a composition with anti-aging effect and an application thereof in preparing cosmetics with anti-aging effect. Background Art

[0002] Skin aging is the result of long-term lack of skin care or the decline of multipotent cells in the body with aging. Dead skin cells cling to the skin's surface and don't shed, causing a series of problems that seriously hinder beauty. Skin aging is a natural process that not only affects facial appearance but can also lead to psychological issues such as depression and low self-esteem, and is even associated with numerous diseases. While we cannot prevent the natural aging of the skin, we can modify its aging process through scientific and effective measures. Therefore, it is important to choose high-quality skin care products that replenish the skin with nutrients, slow water loss, promote cell metabolism, protect the skin barrier function, enhance skin elasticity, scavenge free radicals, and inhibit melanin production to slow the aging process.

[0003] Plant extracts are popular among consumers for their natural, green, and healthy appeal. Many plant extracts can smooth the skin, remove aging wrinkles, and improve skin elasticity, thereby delaying skin aging. For example, CN108524371A discloses a composition and its use in the preparation of cosmetics with anti-aging properties. This invention combines honeysuckle extract, camellia extract, and snow lotus extract with glycerin and other components to form an essence that scavenges free radicals and promotes fibroblast proliferation, thus exhibiting anti-aging properties. For example, CN107823094A discloses an anti-aging skin care composition containing bird's nest extract and its preparation method. The composition contains the following components by weight: 0.15-1.5% bird's nest extract, 2-8% Lysimachia chinensis extract, 3-10% wolfberry leaf extract, 1-12% kudzu root extract, 3-9% dandelion extract, 2-6% moisturizer, 5-12% emulsifier, 0.25-1.5% thickener, 1-15% hydrogenated coconut oil, and the balance deionized water. The composition can inhibit the generation of free radicals, increase skin elasticity, reduce skin fine lines, and reduce skin moisture loss; and has good stability.

[0004] However, most of the anti-aging compositions containing plant extracts disclosed in the prior art have complex formulas, many compound ingredients, and high preparation processes and costs. Therefore, it is very meaningful to develop a product containing plant extracts with a simple formula and significant anti-aging effect. Summary of the Invention

[0005] In view of the deficiencies of the prior art, the object of the present invention is to provide a composition with anti-aging efficacy and its application, specifically to provide a composition with anti-aging efficacy and its application in the preparation of cosmetics with anti-aging efficacy.

[0006] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides a composition with anti-aging efficacy, wherein the composition with anti-aging efficacy comprises Bidens pilosa L. extract and Pansy tricolor extract.

[0008] Among them, Bidens pilosa (Binnula tricolor), the whole herb of Bidens pilosa (Binnula tricolor), a plant of the Asteraceae family, has the effects of detoxifying and reducing swelling, clearing heat and relieving pain, promoting blood circulation and dispersing blood stasis, and regulating qi and eliminating stagnation. It is commonly used for gastroenteritis, abdominal pain caused by heatstroke, bacterial dysentery, fever caused by colds, acute laryngitis, stranguria, leucorrhea, hemorrhoids, rectal prolapse, bloody stools, diabetes, snake bites, periarthritis of the shoulder, traumatic injuries, and arthritis. Pansy, also known as butterfly flower, is a biennial or perennial herb of the genus Viola in the Violaceae family. Its flowers typically come in purple, white, and yellow, hence the name. The whole herb contains quercetin, luteolin, salicylic acid, ferulic acid, and other phenolic alcohols and polysaccharide compounds.

[0009] The present invention creatively combines Bidens pilosa L. extract and Viola tricolor extract to exert anti-aging effects through different mechanism pathways. The two have significant synergistic effects in anti-aging effects, specifically showing significant effects of increasing skin elasticity, anti-oxidation and anti-wrinkle. The composition is very suitable for use in skin care products, is mild and non-irritating, and has good stability.

[0010] Preferably, the composition with anti-aging efficacy comprises 1-8 parts of Bidens pilosa extract (for example, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, etc.) and 0.5-6 parts of Pansy tricolor extract (for example, 0.5 part, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, etc.) by weight.

[0011] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0012] When the above two extracts are combined in a specific mass ratio, they have more significant anti-aging effects.

[0013] Preferably, the Bidens pilosa extract or the Pansy extract are both prepared by a preparation method comprising the following steps:

[0014] The whole herb of Bidens pilosa or Pansy is dried and crushed, the extract powder is mixed with an alcohol-water solution, and subjected to microwave-ultrasonic co-extraction treatment. The treated liquid is centrifuged, and the above extraction process is repeated after precipitation. The supernatant is collected and combined, concentrated to remove alcohol, and freeze-dried to obtain the product.

[0015] If the Bidens pilosa L. extract and the Viola tricolor extract in the composition of the present invention are prepared by the above-mentioned specific extraction process, the obtained extract combination has more significant effects in increasing skin elasticity, anti-oxidation and anti-wrinkle.

[0016] Preferably, the volume fraction of alcohol in the alcohol aqueous solution is 40-90%, for example, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, etc., preferably 60-80%.

[0017] When the volume fraction of alcohol in the alcohol aqueous solution used as the extraction solvent is selected to be 60-80%, it has a better extraction effect than other volume fractions, so that the obtained extract components have better anti-aging effects.

[0018] Preferably, the alcohol is selected from any one of ethanol, 1,3-butanediol, 1,3-propylene glycol, n-butanol, n-propanol or isopropanol, or a combination of at least two thereof.

[0019] Preferably, the alcohol is a combination of ethanol, 1,3-butanediol and 1,3-propylene glycol.

[0020] The present invention also creatively discovered that when the extraction solvent is a combined aqueous solution of ethanol, 1,3-butanediol and 1,3-propylene glycol, it has a better extraction effect than a single type of alcohol aqueous solution, so that the obtained extract components have better anti-aging effects.

[0021] Preferably, the ratio of the extract powder to the alcohol aqueous solution is 1g:(30-80)mL, for example, 1g:30mL, 1g:40mL, 1g:50mL, 1g:60mL, 1g:70mL, 1g:80mL, etc.

[0022] Preferably, the drying refers to hot air drying at 40-60°C (eg, 40°C, 45°C, 50°C, 55°C, 60°C, etc.) for 10-12h (eg, 10h, 11h, 12h, etc.).

[0023] Preferably, the crushing is to 40-80 mesh, such as 40 mesh, 50 mesh, 60 mesh, 70 mesh, 80 mesh, etc.

[0024] Preferably, the power of the microwave in the co-extraction process is 200-800 W, for example, 200 W, 300 W, 400 W, 500 W, 600 W, 700 W, 800 W, etc., and the microwave time is 10-60 s, for example, 10 s, 20 s, 30 s, 40 s, 50 s, 60 s, etc.; the power of the ultrasonic wave is 200-400 W, for example, 200 W, 250 W, 300 W, 350 W, 400 W, etc., and the ultrasonic time is 5-30 min, for example, 5 min, 7 min, 10 min, 12 min, 15 min, 20 min, 22 min, 25 min, 30 min, etc.

[0025] Unlike the more commonly used organic solvent thermal extraction method, the present invention adopts a microwave and ultrasonic co-extraction method at a lower temperature, which is more effective than the extract obtained by single microwave treatment or ultrasonic treatment. Moreover, when microwave treatment and ultrasonic treatment are used within the above-mentioned specific process parameter range, the two have a better coordinated effect.

[0026] Preferably, the co-extraction process is carried out at a constant temperature of 40-80°C (eg, 40°C, 50°C, 60°C, 70°C, 80°C, etc.).

[0027] Preferably, the centrifugation is carried out at a speed of 1500-5000 rpm (e.g., 1500 rpm, 2000 rpm, 2500 rpm, 3000 rpm, 3500 rpm, 4000 rpm, 4500 rpm, 5000 rpm, etc.) for 10-30 min (e.g., 10 min, 15 min, 20 min, 25 min, 30 min, etc.).

[0028] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0029] Preferably, the precipitation is repeated for 0-3 times, for example, 0 times, 1 time, 2 times, 3 times, wherein 0 times means that the precipitation is not performed and the extraction process is no longer repeated, but the precipitation is directly discarded.

[0030] Further preferably, the composition with anti-aging efficacy further comprises glycolipids.

[0031] Further adding glycolipids to the above composition can help further improve the anti-aging effects of Bidens pilosa extract and Pansy tricolor extract.

[0032] Preferably, the composition with anti-aging efficacy comprises, by weight, 1-8 parts of Bidens pilosa extract (e.g., 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, etc.), 0.5-6 parts of Pansy tricolor extract (e.g., 0.5 parts, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, etc.), and 0.03-0.4 parts of glycolipid (e.g., 0.03 parts, 0.1 parts, 0.15 parts, 0.2 parts, 0.25 parts, 0.3 parts, 0.35 parts, 0.4 parts, etc.).

[0033] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0034] The glycolipids mentioned above are calculated based on the actual content of effective ingredients contained in the commercially available raw materials. The commercially available raw materials may also selectively contain any one or a combination of at least two of solvents, fillers, diluents, stabilizers, pH regulators, antibacterial agents, antioxidants or impurities within the permitted range.

[0035] In a second aspect, the present invention provides a method for preparing the composition with anti-aging efficacy described in the first aspect, the preparation method comprising:

[0036] The Bidens pilosa L. extract and the Pansy tricolor extract are prepared separately, and then the prepared Bidens pilosa L. extract and the Pansy tricolor extract are mixed according to a proportion;

[0037] Alternatively, the whole herb of Bidens pilosa and the whole herb of Viola tricolor are weighed in proportion and mixed, dried and crushed together, the extract powder is mixed with an alcohol-water solution, and subjected to microwave-ultrasonic co-extraction treatment, the treated liquid is centrifuged, the precipitation is repeated with the above extraction process, the supernatant is collected and combined, and the alcohol is concentrated to obtain the product.

[0038] In a third aspect, the present invention provides a cosmetic with anti-aging efficacy, comprising the composition with anti-aging efficacy described in the first aspect.

[0039] Preferably, the mass percentage of the composition with anti-aging efficacy in the cosmetic is 0.001-5% in terms of solid content, for example, 0.001%, 0.005%, 0.008%, 0.01%, 0.03%, 0.05%, 0.1%, 0.3%, 0.5%, 1%, 2%, 3%, 4%, 5%, etc. Other specific point values ​​within this numerical range can be selected and will not be repeated here.

[0040] Compared with the prior art, the present invention has the following beneficial effects:

[0041] The present invention creatively combines Bidens pilosa L. extract and Viola tricolor extract to exert anti-aging effects through different mechanism pathways. The two have significant synergistic effects in anti-aging effects, specifically showing significant effects of increasing skin elasticity, anti-oxidation and anti-wrinkle. The composition is very suitable for use in skin care products, is mild and non-irritating, and has good stability. DETAILED DESCRIPTION

[0042] In order to further illustrate the technical means and effects adopted by the present invention, the technical solutions of the present invention are further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0043] The whole herb of Bidens pilosa L. involved in the following embodiments, comparative examples, application examples, and comparative application examples was purchased from Guangdong Fudonghai Pharmaceutical Co., Ltd.; the whole herb of Pansy tricolor was purchased from Guangzhou Bisheng Trading Co., Ltd.; and the glycolipid was a product model CERAMELA (olive oil fermentation ceramide) purchased from Shanghai Yankai Biotechnology Co., Ltd.

[0044] Example 1

[0045] This embodiment provides a composition with anti-aging efficacy, which is obtained by uniformly mixing 10 parts of Bidens pilosa extract and 5 parts of Pansy tricolor extract, in parts by weight, wherein the Bidens pilosa extract and Pansy tricolor extract are prepared by the following method:

[0046] (1) The whole herb of Bidens pilosa and the whole herb of Pansy were dried by hot air at 50°C for 12 hours, and then crushed to 60 mesh to obtain Bidens pilosa powder and Pansy powder;

[0047] (2) Bidens pilosa powder and Pansy tricolor powder were mixed with 75% ethanol aqueous solution at a material-liquid ratio of 1 g:50 mL, and then subjected to microwave-ultrasonic co-extraction at a constant temperature of 50°C, with a microwave power of 400 W and a microwave time of 30 s; the ultrasonic power was 300 W and the ultrasonic time was 25 min;

[0048] (3) The treated liquid was centrifuged at 3500 rpm for 25 min to obtain a supernatant and a precipitate. The supernatant was collected and the precipitate was subjected to the extraction process of step (2) and the centrifugation process of step (3) twice. The supernatants were collected and combined, and finally concentrated to remove alcohol and freeze-dried to obtain Bidens pilosa extract and Pansy extract.

[0049] Example 2

[0050] This embodiment provides a composition with anti-aging efficacy, which is obtained by uniformly mixing 5 parts of Bidens pilosa extract and 3 parts of Pansy extract, in parts by weight, wherein the Bidens pilosa extract and Pansy extract are prepared by the following method:

[0051] (1) The whole herb of Bidens pilosa and the whole herb of Pansy were dried by hot air at 50°C for 12 hours, and then crushed to 80 mesh to obtain Bidens pilosa powder and Pansy powder;

[0052] (2) Bidens pilosa powder and Pansy tricolor powder were mixed with 60% ethanol aqueous solution at a material-liquid ratio of 1 g:80 mL, and then subjected to microwave-ultrasonic co-extraction at a constant temperature of 65°C, with a microwave power of 700 W and a microwave time of 40 s; the ultrasonic power was 200 W and the ultrasonic time was 30 min;

[0053] (3) The treated liquid was centrifuged at 4500 rpm for 15 min to obtain a supernatant and a precipitate. The supernatant was collected and the precipitate was subjected to the extraction process of step (2) and the centrifugation process of step (3) twice. The supernatants were collected and combined, and finally concentrated to remove alcohol and freeze-dried to obtain Bidens pilosa extract and Pansy extract.

[0054] Example 3

[0055] This embodiment provides a composition with anti-aging efficacy, which is obtained by uniformly mixing 4 parts of Bidens pilosa extract and 3 parts of Pansy extract, in parts by weight, wherein the Bidens pilosa extract and Pansy extract are prepared by the following method:

[0056] (1) The whole herb of Bidens pilosa and the whole herb of Pansy were dried by hot air at 50°C for 12 hours, and then crushed into 50 mesh to obtain Bidens pilosa powder and Pansy powder;

[0057] (2) Bidens pilosa powder and Pansy tricolor powder were mixed with 80% ethanol aqueous solution at a material-liquid ratio of 1 g:30 mL, and then subjected to microwave-ultrasonic co-extraction at a constant temperature of 45°C, with a microwave power of 300 W and a microwave time of 60 s; the ultrasonic power was 400 W and the ultrasonic time was 10 min;

[0058] (3) The treated liquid was centrifuged at 4500 rpm for 15 min to obtain a supernatant and a precipitate. The supernatant was collected and the precipitate was subjected to the extraction process of step (2) and the centrifugation process of step (3) twice. The supernatants were collected and combined, and finally concentrated to remove alcohol and freeze-dried to obtain Bidens pilosa extract and Pansy extract.

[0059] Example 4

[0060] This embodiment provides a composition with anti-aging efficacy, which is obtained by uniformly mixing 10 parts of Bidens pilosa extract and 5 parts of Pansy extract, in parts by weight. The preparation methods of the Bidens pilosa extract and Pansy extract are different from those in Example 1 only in that: (2) Bidens pilosa powder and Pansy powder are respectively mixed with 75% ethanol aqueous solution at a material-liquid ratio of 1 g:50 mL, and then subjected to a single microwave treatment at a constant temperature of 50° C., a microwave power of 400 W, and a microwave time of 40 s. The other steps remain unchanged.

[0061] Example 5

[0062] This embodiment provides a composition with anti-aging efficacy, which is obtained by uniformly mixing 10 parts of Bidens pilosa extract and 5 parts of Pansy extract, in parts by weight. The preparation methods of the Bidens pilosa extract and Pansy extract are different from those in Example 1 only in that: (2) Bidens pilosa powder and Pansy powder are respectively mixed with 75% ethanol aqueous solution at a material-liquid ratio of 1 g:50 mL, and then subjected to a single ultrasonic treatment at a constant temperature of 50° C., the ultrasonic power is 300 W, and the ultrasonic time is 40 minutes. The other steps remain unchanged.

[0063] Example 6

[0064] This embodiment provides a composition with anti-aging efficacy, which is obtained by uniformly mixing 10 parts of Bidens pilosa extract and 5 parts of Pansy extract by weight, wherein the preparation method of Bidens pilosa extract and Pansy extract is different from that of Example 1 only in that: (2) Bidens pilosa powder and Pansy powder are respectively mixed with 75% ethanol aqueous solution at a material-liquid ratio of 1 g:50 mL, and then subjected to microwave-ultrasound co-extraction treatment at a constant temperature of 50°C, with a microwave power of 900 W and a microwave time of 70 s; and the ultrasonic power of 300 W and an ultrasonic time of 25 min. The other steps remain unchanged.

[0065] Example 7

[0066] This embodiment provides a composition with anti-aging efficacy, which is obtained by uniformly mixing 10 parts of Bidens pilosa extract and 5 parts of Pansy extract by weight, wherein the preparation method of Bidens pilosa extract and Pansy extract is different from that of Example 1 only in that: (2) Bidens pilosa powder and Pansy powder are respectively mixed with 75% ethanol aqueous solution at a material-liquid ratio of 1 g:50 mL, and then subjected to microwave-ultrasound co-extraction treatment at a constant temperature of 50°C, with a microwave power of 400 W and a microwave time of 30 s; and the ultrasonic power of 500 W and an ultrasonic time of 40 min. The other steps remain unchanged.

[0067] Example 8

[0068] This embodiment provides a composition with anti-aging efficacy, which is obtained by uniformly mixing 10 parts of Bidens pilosa extract and 5 parts of Pansy tricolor extract, in parts by weight, wherein the Bidens pilosa extract and Pansy tricolor extract are prepared by the following method:

[0069] (1) The whole herb of Bidens pilosa and the whole herb of Pansy were dried by hot air at 50°C for 12 hours, and then crushed to 60 mesh to obtain Bidens pilosa powder and Pansy powder;

[0070] (2) Bidens pilosa powder and Pansy tricolor powder were mixed with 75% ethanol aqueous solution at a solid-liquid ratio of 1 g:50 mL, and then heated under reflux for 25 min;

[0071] (3) The extracts were centrifuged at 3500 rpm for 25 min to obtain a supernatant and a precipitate. The supernatant was collected and the precipitate was subjected to the extraction process of step (2) and the centrifugation process of step (3) twice. The supernatants were collected and combined, and finally concentrated to remove alcohol and freeze-dried to obtain Bidens pilosa extract and Pansy extract.

[0072] Example 9

[0073] This embodiment provides a composition with anti-aging efficacy, which is obtained by uniformly mixing 10 parts of Bidens pilosa extract, 5 parts of Pansy extract and 3 parts of glycolipid, in parts by weight. The preparation methods of Bidens pilosa extract and Pansy extract are the same as those in Example 1.

[0074] Comparative Example 1

[0075] This comparative example provides a product with anti-aging efficacy, which is a single Bidens pilosa L. extract, and its preparation method is the same as that of Example 1.

[0076] Comparative Example 2

[0077] This comparative example provides a product with anti-aging efficacy, which is a single pansy extract, and its preparation method is the same as that of Example 1.

[0078] Test Example 1

[0079] Chicken embryo chorioallantoic membrane vascular test:

[0080] (1) Experimental conditions

[0081] Chicken embryos: SPF-grade Bailaihang chickens were purchased from Beijing Merial Vital Laboratory Animal Technology Co., Ltd.

[0082] Culture conditions: incubator temperature 37.5±0.5℃, relative humidity 55%-70%.

[0083] Reagents and controls: Positive control (PC1): 1.0% SDS solution;

[0084] Positive control (PC2): 0.1 mol / L NaOH solution;

[0085] Negative control (NC): 0.9% NaCl solution;

[0086] Test sample (TA): products prepared in Examples 1-9 and Comparative Examples 1-2.

[0087] (2) Experimental methods

[0088] CAM preparation: Purchase 0-day-old chicken embryos, 50-60g. During the incubation process, turn the embryos over once a day. When the embryos are 5 days old, check their developmental status. On the 6th day, peel off the eggshell to expose the white egg membrane; carefully remove the inner membrane with tweezers to ensure that the vascular membrane is not damaged.

[0089] Formal test: For each group of six chick embryos, the test substance was ground into a powder. After gentle squeezing into a 0.3 mL volume in a graduated container (e.g., a microcentrifuge tube), the powder was applied directly to the CAM, ensuring that at least 50% of the CAM surface was covered by the test substance. After 3 minutes of application, the test substance on the CAM membrane was gently rinsed with normal saline. This rinse may quickly mask any slight bleeding changes on the CAM membrane. Therefore, the results should be observed after 30 seconds of rinsing. The extent of each toxic effect, including bleeding, coagulation, and vascular lysis, was observed to the nearest second, and the extent of the reaction was recorded. The same procedure was used for the positive and negative controls.

[0090] Data Analysis: The severity of each reaction was recorded, graded, and scored. Bleeding was categorized as no bleeding (0), mild bleeding (1), moderate bleeding (2), and severe bleeding (3) based on severity. Coagulation was categorized as no coagulation (0), mild coagulation (1), moderate coagulation (2), and severe coagulation (3). Vasolysis was categorized as no vascular lysis (0), mild vascular lysis (1), moderate vascular lysis (2), and severe vascular lysis (3). The endpoint score (ES) was calculated according to the following formula, with the result rounded to two decimal places: score per embryo = sum of the observed bleeding, coagulation, and vascular lysis levels for each embryo; ES = mathematical sum of the scores for all six embryos. The eye irritation of the test substances was classified according to the ES values ​​as shown in Table 1.

[0091] Table 1

[0092]

[0093]

[0094] The endpoint scores ES values ​​of the tested samples are shown in Table 2.

[0095] Table 2

[0096] Group End point score Group End point score Positive control 1 18 Positive control 2 17 Negative control 0 Example 1 0 Example 2 1 Example 3 2 Example 4 4 Example 5 3 Example 6 5 Example 7 4 Example 8 5 Example 9 5 Comparative Example 1 8 Comparative Example 2 9

[0097] As can be seen from the data in Table 2, the composition with anti-aging efficacy of the present invention is classified as non-irritating. Compared with Comparative Examples 1-2, Examples 1-9 can effectively reduce its irritation.

[0098] Test Example 2

[0099] Skin elasticity evaluation test:

[0100] (1) Test principle: Based on the suction-stretching principle, a negative pressure is generated on the surface of the skin being tested to suck the skin into a specific test probe. The depth to which the skin is sucked into the test probe is measured by a non-contact optical test system. The test probe includes a light transmitter and a receiver. The ratio of the emitted light to the received light is proportional to the depth of the skin being sucked into the skin. The elasticity of the skin is determined by measuring the size of the skin retraction after negative pressure suction (represented by the parameter R). The closer the R value is to 1, the better the skin elasticity. This can be used to analyze the elastic properties of the skin. The skin elasticity value R = U1 / U2, where U2 represents the maximum stretching of the skin and U1 represents the amount of recovery of the skin after the negative pressure is removed.

[0101] (2) Subjects: A total of 120 volunteers (24 males and 96 females) aged 18-50 years, who were not hypersensitive, were randomly divided into 12 groups, with 2 males and 8 females in each group.

[0102] Materials: dry facial tissue, latex gloves, pipette.

[0103] Instrument: German Courage+Khazaka skin elasticity tester MPA-580.

[0104] Test parameters: constant negative pressure mode; 3 cycles, 2 seconds each, constant negative pressure in the first second, and elimination of negative pressure in the second.

[0105] Preparation before the test: The subject should mark the measurement area on the inner side of the forearms of both hands. The experimental area should be at least 3cm×3cm, and the interval between each test area should be at least 1cm.

[0106] Preparation of test samples: 93 parts of deionized water were heated to 60°C and mixed with 5 parts of the products prepared in Examples 1-9 and Comparative Examples 1-2, and stirred until transparent. The temperature was then lowered to 25°C, mixed with 2 parts of pentanediol, stirred, and passed through a 500-mesh gauze.

[0107] (3) Test process: 11 groups of test samples were placed at 2 ml / cm2 Apply a single application of the desired dosage (pure water was used as the blank control). Using latex gloves, apply the sample evenly to the test area and gently pat until absorbed. Use once daily for two weeks before measuring the results. Touch the probe vertically to the test area to begin the test, and remove the probe after the test cycle is complete. Measure three points on each test area. The results are shown in Table 3.

[0108] Table 3

[0109]

[0110]

[0111] As can be seen from the data results in Table 3, the composition of the present invention has a good effect of restoring skin elasticity, wherein the Bidens pilosa extract and the Pansy tricolor extract promote each other in terms of skin elasticity and work together to achieve the optimal effect.

[0112] Test Example 3

[0113] Anti-wrinkle effect evaluation test:

[0114] (1) Experimental principle: The anti-wrinkle test uses the VISIA-CR facial skin image analyzer. The VISIA skin image analyzer uses multi-spectral imaging technology. Through a 12-megapixel camera, it can shoot from three angles: the front, left side, and right side. It can also be imaged three times under three light sources: standard white light, cross-sectional polarized light, and ultraviolet light. Therefore, the VISIA facial image analysis system can systematically analyze the pigmentation spots, texture, pores, wrinkles, ultraviolet light spots, stable pigment spots, red areas, and rhododendrons on the subject's facial skin, providing an accurate and quantitative analysis basis for the skin. By recording the pigmentation spots, wrinkles, and texture on the subject's face before and after the test, the absolute score represents the area and intensity of the skin feature detection value, which is used to observe the changes in skin properties. Therefore, the data obtained by the absolute score (the average of the test results of the front, left side, and right side) is selected as the analysis standard and data statistical basis. All the data of each subject are input into the computer, the measurement results are compared, and the subject's skin characteristics and various index data are analyzed through statistical processing.

[0115] (2) Subjects: 120 volunteers (24 males and 96 females) aged 18-50 years old and not hypersensitive were randomly divided into 12 groups, with 2 males and 8 females in each group.

[0116] Instrument: VISIA facial image analysis system (CANFIELD Technology, USA).

[0117] Preparation of test samples: 93 parts of deionized water were heated to 60°C and mixed with 5 parts of the products prepared in Examples 1-9 and Comparative Examples 1-2, and stirred until transparent. The temperature was then lowered to 25°C, mixed with 2 parts of pentanediol, stirred, and passed through a 500-mesh gauze.

[0118] (3) Test process: Each group of volunteers used 11 groups of the above test samples and were required to use them continuously, applying the products twice a day, morning and evening, for 28 consecutive days, and required a follow-up visit on the 28th day of the test (D28). The test of the same subject was completed by the same measurement personnel. No products (cosmetics, external medicines, or internal health care products) should be used on the test area for 15 days before the test. After thoroughly cleaning the face with a uniform cleansing product, gently wipe it dry with a paper towel, and wait for 15 minutes in a room with a room temperature of 22℃±2℃ and a relative humidity of 40±2%, take a photo of the test subject's face with a VISIA facial image analyzer. The analysis area should try to avoid interference from reflective areas and hair. The subject's face (including the front, left side, and right side) was photographed, and the absolute scores obtained were statistically analyzed to record the detection data of the subject's facial wrinkles. The test results are shown in Table 4.

[0119] Table 4

[0120]

[0121]

[0122] The data in Table 4 demonstrate that the composition of the present invention exhibits excellent anti-wrinkle efficacy, with the Bidens pilosa extract and the Pansy tricolor extract mutually enhancing their anti-wrinkle efficacy, resulting in a synergistic effect. Furthermore, the preparation processes of the Bidens pilosa extract and the Pansy tricolor extract also influence the anti-wrinkle efficacy of the composition.

[0123] Test Example 4

[0124] Antioxidant effect evaluation test:

[0125] (1) Preparation of test samples: First, the products prepared in Examples 1-9 and Comparative Examples 1-2 were prepared into test samples with a concentration of 1%.

[0126] (2) Test procedure: Add 1.0 mL of 40 mg / L DPPH test solution and 1.0 mL of 1% test sample to a test tube, add anhydrous ethanol to the total volume to 3 mL, shake well, react in the dark for 30 minutes, and measure the absorbance at 517 nm as Ai; add 1.0 mL of 40 mg / L DPPH test solution and 2 mL of anhydrous ethanol to the test tube, shake well, react in the dark for 30 minutes, and measure the absorbance as Ac; add 2 mL of anhydrous ethanol and 1.0 mL of 1% test sample to the test tube, shake well, react in the dark for 30 minutes, and measure the absorbance as Aj.

[0127] The free radical scavenging rate was calculated according to the following formula.

[0128] Clearance (%) = [1-(Ai-Aj) / Ac] × 100%

[0129] Among them, Aj is the contribution of the reaction sample itself to the absorbance; Ai is the absorbance value of the sample after the action of DPPH; Ac is the absorption of DPPH itself at the measurement wavelength.

[0130] The greater the DPPH scavenging rate, the stronger the free radical scavenging antioxidant capacity. The results are shown in Table 5 (each group of samples was measured in parallel 5 times, and the data are presented as average values).

[0131] Table 5

[0132] Group Free radical scavenging rate (%) Example 1 92.6 Example 2 91.3 Example 3 91.6 Example 4 87.6 Example 5 88.2 Example 6 90.5 Example 7 90.8 Example 8 89.9 Example 9 94.8 Comparative Example 1 50.4 Comparative Example 2 53.8

[0133] The data in Table 5 demonstrate that the composition of the present invention exhibits excellent free radical scavenging and antioxidant effects, with the Bidens pilosa extract and Pansy extract mutually enhancing their antioxidant efficacy, resulting in a synergistic effect. Furthermore, the preparation processes of the Bidens pilosa extract and Pansy extract also influence the antioxidant efficacy of the composition.

[0134] The applicant states that while the present invention uses the aforementioned embodiments to illustrate a composition with anti-aging efficacy and its application, the present invention is not limited to these embodiments, nor does it necessarily rely on these embodiments for implementation. Those skilled in the art should understand that any improvements to the present invention, equivalent substitutions for various raw materials in the present invention, addition of auxiliary ingredients, and selection of specific methods, etc., fall within the scope of protection and disclosure of the present invention.

[0135] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the scope of protection of the present invention.

[0136] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any appropriate manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A composition with anti-aging effect, characterized in that: The anti-aging composition comprises, by weight, 1-8 parts of Bidens pilosa extract and 0.5-6 parts of Pansy tricolor extract; The Bidens pilosa L. extract or the Pansy tricolor extract is prepared by a preparation method comprising the following steps: drying and crushing the whole herb of Bidens pilosa L. or the whole herb of Pansy tricolor, mixing the extract powder with an alcohol-water solution, subjecting the extracted powder to microwave-ultrasound co-extraction, centrifuging the treated liquid, precipitating, repeating the above extraction process, collecting and combining the supernatants, concentrating to remove alcohol, and freeze-drying to obtain the extract; The power of the microwave in the co-extraction process is 200-800 W, and the microwave time is 10-60 s; the power of the ultrasonic wave is 200-400 W, and the ultrasonic time is 5-30 min; the co-extraction process is carried out at a constant temperature of 40-80°C.

2. The composition with anti-aging efficacy according to claim 1, characterized in that The volume fraction of alcohol in the alcohol aqueous solution is 40-90%.

3. The composition with anti-aging efficacy according to claim 2, characterized in that The volume fraction of alcohol in the alcohol aqueous solution is 60-80%.

4. The composition with anti-aging efficacy according to claim 2, characterized in that The alcohol is selected from any one of ethanol, 1,3-butanediol, 1,3-propylene glycol, n-butanol, n-propanol or isopropanol, or a combination of at least two thereof.

5. The composition with anti-aging efficacy according to claim 4, characterized in that: The alcohol is a combination of ethanol, 1,3-butanediol and 1,3-propylene glycol.

6. The composition with anti-aging efficacy according to claim 1, characterized in that The ratio of the extract powder to the alcohol aqueous solution is 1 g: (30-80) mL.

7. The composition with anti-aging efficacy according to claim 1, characterized in that The drying refers to hot air drying at 40-60° C. for 10-12 hours.

8. The composition with anti-aging efficacy according to claim 1, characterized in that: The crushing is to 40-80 mesh.

9. The composition with anti-aging efficacy according to claim 1, characterized in that: The centrifugation is carried out at a rotation speed of 1500-5000 rpm for 10-30 min.

10. The composition with anti-aging efficacy according to claim 1, characterized in that: The precipitation is repeated for 0-3 times.

11. The composition with anti-aging efficacy according to claim 1, characterized in that: The composition with anti-aging efficacy also includes glycolipids.

12. The composition with anti-aging efficacy according to claim 11, characterized in that: The composition with anti-aging efficacy comprises, by weight, 1-8 parts of Bidens pilosa extract, 0.5-6 parts of Pansy extract and 0.03-0.4 parts of glycolipid.

13. A cosmetic with anti-aging effect, characterized in that: The cosmetic comprises the composition with anti-aging efficacy according to any one of claims 1 to 12.

14. The cosmetic according to claim 13, characterized in that The mass percentage of the composition with anti-aging effect in the cosmetic is 0.001-5% based on solid content.

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