Cosmetic composition for skin elasticity enhancement, skin wrinkle alleviation, and skin whitening, comprising fermented extract of perilla frutescens var. acuta kudo as active ingredient

Abstract

The present invention relates to a cosmetic composition for skin elasticity enhancement, skin wrinkle alleviation, and skin whitening, comprising a fermented extract of Perilla frutescens var. acuta Kudo as an active ingredient. The cosmetic composition comprising a fermented extract of Perilla frutescens var. acuta Kudo as an active ingredient, according to one embodiment of the present invention, comprises components derived from natural products and thus is harmless to the human body and exhibits remarkable efficacy in increasing collagen production and ability to inhibit melanin production, thereby being useful for skin elasticity enhancement, skin wrinkle alleviation, and skin whitening.

Description

Cosmetic composition for improving skin elasticity, improving skin wrinkles, and whitening, containing fermented extract of chajigi as an active ingredient

[0001] The present invention relates to a cosmetic composition for improving skin elasticity, improving skin wrinkles, and whitening skin, which contains a fermented extract of Chajigi as an effective ingredient.

[0002] This research was conducted with the support of the Gyeonggi-do Regional Cooperation Research Center (Project No. GRRCAjou2023-B01).

[0003] With the recent rise in living standards and increased life expectancy, the desire to maintain healthy, youthful skin is on the rise, leading to a continued surge in interest in cosmetics with anti-aging and whitening properties. Wrinkles, a hallmark of skin aging, are primarily caused by UV exposure and the natural aging process. They result from decreased synthesis and accelerated breakdown of collagen, a key protein in the dermal layer, leading to a decline in skin elasticity. Skin fibroblasts are the primary cells responsible for producing collagen, and stimulating their collagen synthesis is a key strategy for improving wrinkles. Furthermore, skin whitening aims to alleviate pigmentation, such as freckles and dark spots, to achieve a clear and even skin tone. These pigmentations are caused by excessive melanin production in melanocytes, triggered by external stimuli such as UV rays. Therefore, inhibiting specific steps in the melanin production process can provide effective whitening effects. To achieve these wrinkle-improving and whitening effects, various functional ingredients such as retinol, ascorbic acid, arbutin, and kojic acid have been used in cosmetics. However, some of these ingredients have low stability, easily deteriorating under light or heat, or can cause skin irritation. Consequently, there is a growing demand for new, naturally derived functional ingredients that are safe for the human body while simultaneously exhibiting excellent wrinkle-improving and whitening effects.

[0004] Perilla frutescensvar. acutakudo is a plant belonging to the Lamiaceae family and has been traditionally used for food and medicinal purposes. Meanwhile, fermentation technology using specific microorganisms is known as a method to increase the absorption rate of the active ingredients of raw materials in the body, alleviate the inherent toxicity of the raw material, and produce new physiologically active substances to enhance efficacy. Representatively, Korean Patent No. 10-2156917 confirmed the excellent effect of a fermented extract of Perilla frutescens on relieving stress-induced depression, anxiety, or tension, thereby producing a pharmaceutical composition that has the effect of preventing, treating, or improving diseases such as stress-induced tension, stress-induced anxiety, and stress-induced depression. However, there is a lack of research on the skin improvement effect of this fermented extract of Perilla frutescens.

[0005] Under the circumstances described above, the inventors of the present invention confirmed that a fermented extract of perilla leaves, prepared by extracting perilla leaves in hot water and then fermenting it with Bacillus subtilis, concentration-dependently promotes collagen synthesis in skin fibroblasts. Simultaneously, the inventors confirmed that it effectively inhibits melanin production without cytotoxicity toward melanoma-derived cells (B16F10) and exhibits a strong whitening effect similar to that of the positive control, arbutin, at a concentration of 30 μg / mL or higher, thereby completing the present invention.

[0006] Accordingly, the purpose of the present invention is to provide a cosmetic composition for improving skin elasticity, improving skin wrinkles, and whitening skin, which contains a fermented extract of chazugi (Perilla frutescensvar. acutakudo) as an effective ingredient.

[0007] The terminology used herein is for the purpose of description only and should not be construed as limiting. The singular expression includes the plural expression unless the context clearly indicates otherwise. In this specification, the terms "comprises" or "has" and the like are intended to specify the presence of a feature, number, step, operation, component, part, or combination thereof described in the specification, but should be understood to not preclude the presence or addition of one or more other features, numbers, steps, operations, components, parts, or combinations thereof.

[0008] Additionally, unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by a person of ordinary skill in the art to which the embodiments pertain. Terms defined in commonly used dictionaries should be interpreted as having a meaning consistent with their meaning in the context of the relevant technology, and shall not be interpreted in an idealized or overly formal sense unless explicitly defined herein.

[0009] In the present invention, duplicate content or terms have been omitted to avoid excessive complexity of this specification.

[0010] Hereinafter, the present invention will be described in detail.

[0011]

[0012] In order to achieve the above object, according to one aspect of the present invention, a cosmetic composition for improving skin elasticity, improving skin wrinkles, and whitening skin is provided, which contains a fermented extract of chazugi (Perilla frutescensvar. acutakudo) as an effective ingredient.

[0013] According to one embodiment of the present invention, the fermented extract may be extracted from one or more parts selected from the group consisting of leaves, stems, flowers, fruits, and whole plants of the tea plant.

[0014] According to one embodiment of the present invention, the fermented extract may be extracted using water, an organic solvent, or a mixture thereof as a solvent and then fermented using a fermentation strain.

[0015] According to one embodiment of the present invention, the fermentation strain may be Bacillus subtilis.

[0016] According to one embodiment of the present invention, the Bacillus subtilis is at least one Bacillus selected from the group consisting of Bacillus subtilis, Bacillus thuringiensis, Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus stearothermophilus, Bacillus coagulans, Bacillus longum, Bacillus pumilus, Bacillus brevis, Bacillus circulans, and Bacillus polymyxa, and preferably Bacillus subtilis.

[0017] According to one embodiment of the present invention, the fermentation may be performed at 5°C to 80°C for 30 minutes to 10 days.

[0018] According to one embodiment of the present invention, the tea extract fermentation product may be included in an amount of 0.001 to 10 wt%, preferably 0.001 to 5 wt%, and most preferably 0.001 to 3 wt%, based on the total composition weight.

[0019] In addition to the above-described effective ingredients, the cosmetic composition of the present invention may further include conventional auxiliary ingredients and carriers, such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and fragrances, which are commonly used in cosmetic compositions. For example, the cosmetic composition may further include auxiliary ingredients such as glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate, and allantoin.

[0020] Since the cosmetic composition of the present invention is basically applied to the skin, it can be manufactured into any formulation that is commonly manufactured with reference to cosmetic compositions in the art. For example, it can be formulated into a suspension, solution, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream, and spray, but is not limited thereto. More specifically, it can be manufactured into a formulation of a flexible toner, a nourishing toner, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, cleansing water, a mask pack, a spray, or a powder.

[0021] When the formulation of the present invention is a paste, cream or gel, the carrier component may include animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc.

[0022] When the formulation of the present invention is a powder or spray, the carrier component may include lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc., and in particular, when it is a spray, it may additionally include a propellant such as chlorofluorohydrocarbon, propane / butane, dimethyl ether, etc.

[0023] When the formulation of the present invention is a solution or emulsion, the carrier component may include a solvent, a solubilizer, an emulsifier, etc., and specifically, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, fatty acid ester of sorbitan, etc.

[0024] When the formulation of the present invention is a suspension, the carrier component may include a liquid diluent such as water, ethanol, or propylene glycol; a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, or polyoxyethylene sorbitan ester; microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tragacanth.

[0025] When the formulation of the present invention is a surfactant-containing cleansing agent, the carrier component may include fatty alcohol sulfate, fatty alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, ethoxylated glycerol fatty acid ester, etc.

[0026] A cosmetic composition comprising a fermented extract of chajigi according to one embodiment of the present invention as an active ingredient is a natural ingredient that is harmless to the human body and exhibits a remarkable effect of increasing collagen production and inhibiting melanin production, and thus can be usefully used for purposes such as improving skin elasticity, improving skin wrinkles, and whitening skin.

[0027] Figure 1 is a diagram showing the results of evaluating cell viability according to concentration of a fermented tea extract according to one embodiment of the present invention.

[0028] FIG. 2 is a diagram showing the results of evaluating the collagen synthesis ability of a fermented extract of chazuki in Detroit 551 cells, which are human-derived fibroblasts, according to one embodiment of the present invention.

[0029] FIG. 3 is a diagram showing the results of evaluating the melanin production inhibition ability of a fermented extract of chazzi in B16F10 cells, which are melanoma-derived cells, according to one embodiment of the present invention.

[0030] Hereinafter, the present invention will be described in more detail through examples. The following examples are intended solely to illustrate the present invention more specifically, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples, in accordance with the gist of the present invention.

[0031]

[0032] Example 1. Preparation of hot water extract of Chajigi and fermented extract of Chajigi

[0033] Perilla frutescensvar. acutakudo was extracted with hot water at 80°C and then sterilized at 121°C for 20 minutes to prepare a perilla hot water extract. Afterwards, Bacillus subtilis was inoculated as a subtilis into the sterilized perilla hot water extract, and the mixture was fermented for 5 days while shaking at 140 rpm in an environment of 25°C and 80% humidity to prepare a fermented perilla extract.

[0034]

[0035] Example 2. Cell viability evaluation

[0036] In order to confirm the cytotoxicity of the fermented extract of the tea tree according to the present invention, cell viability was evaluated through an MTT assay.

[0037] Specifically, B16F10 cells, which are melanoma-derived cells, were seeded at 1 × 10 in a 96-well plate. 4

[0038] After dispensing cells / well at 100 μL / well, the cells were cultured in a 37°C, 5% CO2 incubator for one day. The next day, the cells were washed with PBS and replaced with serum-free medium. Afterwards, the sample (1 to 300 μg / mL of fermented extract of Chazuki) was treated and cultured in a 37°C, 5% CO2 incubator for 24 hours. After the incubation was complete, 100 μL of fresh medium containing MTT reagent (final concentration 0.5 mg / mL) was added to each well and incubated in a 37°C, 5% CO2 incubator for 3 hours. After the reaction, the medium was removed, and 100 μL of DMSO was added to each well and incubated at room temperature for 10 minutes to sufficiently dissolve the purple formazan crystals. Afterwards, the absorbance (OD) value was measured at 570 nm using a multifunctional plate reader, and the cell viability was calculated. The results of evaluating the cell viability according to the concentration of the fermented extract of Chazgi using this method are shown in Figure 1.

[0039] As shown in Fig. 1, at concentrations of 1 to 300 μg / mL, the cell viability was over 90% without a significant decrease compared to the control group (CTL), confirming that the sample did not induce cytotoxicity.

[0040] Therefore, it was confirmed that the fermented extract of Chazuki can be used within a safe concentration range that excludes the effect of cytotoxicity in the collagen synthesis ability evaluation and melanin production inhibition ability evaluation described later.

[0041]

[0042] Example 3. Evaluation of collagen synthesis ability

[0043] In order to evaluate the collagen synthesis ability of the fermented extract of Chazuki according to the present invention, Detroit 551 cells, which are human-derived fibroblasts, were seeded at 1 × 10 in a 96-well plate. 4 After dispensing cells / well at 100 μL / well, the cells were cultured in a 37°C, 5% CO2 incubator for one day. The next day, the cells were washed with PBS and replaced with serum-free medium. Afterwards, the cells were treated with samples (1 to 100 μg / mL of fermented extract of Chazuki or 1 mM Ascorbic acid) and cultured in a 37°C, 5% CO2 incubator for 24 hours. After the incubation was complete, 100 μL of the Antibody-PoD conjugate solution was added to the well, followed by 20 μL each of the culture medium and standard solution, and incubated for an additional 3 hours in a 37°C, 5% CO2 incubator. After the incubation was complete, the culture medium was removed from the well, washed four times with 400 μL of PBS, 100 μL of the chromogenic reagent was added, and incubated at room temperature for 15 minutes. Then, 100 μL of 1 N sulfuric acid was added and reacted. Afterwards, the absorbance (OD) value was measured at 450 nm using a multifunctional plate reader, and the collagen synthesis ability was calculated based on this. The results of evaluating the collagen synthesis ability of the fermented extract of Chajigi using this method are shown in Fig. 2.

[0044] As shown in Fig. 2, the fermented extract of Chazuki increased collagen production in a concentration-dependent manner, and in particular, it was confirmed that the concentrations of 30 and 100 μg / mL showed a collagen production induction effect comparable to that of the positive control, Ascorbic acid.

[0045]

[0046] Example 4. Evaluation of melanin production inhibition ability

[0047] In order to evaluate the melanin production inhibition ability of the fermented extract of Chajigi according to the present invention, 1 × 10 B16F10 cells, which are melanoma-derived cells, were seeded in a 6-well plate. 4 After dispensing 2 mL / well of cells / well, the cells were cultured in a 37°C, 5% CO2 incubator for one day. The next day, the cells were washed with PBS, and fresh medium was supplemented with α-MSH (final concentration 200 nM) and the sample (1–100 μg / mL of fermented extract of Chazuki or 100 μM of Arbutin) and treated together. The cells were then cultured for 72 hours at 37°C, 5% CO2 conditions.

[0048] After incubation, the cells were washed with PBS, harvested with trypsin, and centrifuged at 12,000 rpm for 5 minutes to obtain a cell pellet. 100 μL of a 1 N NaOH + 10% DMSO solution was added to the cell pellet and heated at 80°C for 1 hour. After heating, the pellet was cooled to room temperature and centrifuged at 12,000 rpm for 10 minutes to obtain the supernatant. 100 μL of the supernatant was transferred to a 96-well plate, and the absorbance was measured at 405 nm using a multifunctional plate reader, thereby calculating the melanin production inhibition activity. The results of evaluating the melanin production inhibition activity of the fermented extract of Chajigi in this manner are shown in Figure 3.

[0049] As shown in Fig. 3, α-MSH treatment (VEH) significantly increased melanin production compared to the control group, but the fermented extract of Chajiki inhibited melanin production in a concentration-dependent manner, and it was confirmed that a statistically significant melanin production inhibition effect was observed starting from a concentration of 30 μg / mL or higher. In particular, at concentrations of 30 and 100 μg / mL, the melanin production inhibition rate exceeded 100%, showing an effect comparable to that of 100 μM Arbutin, the positive control group.

[0050]

[0051] Through the above results, it was confirmed that the fermented extract of the present invention not only exhibits effects such as improving skin elasticity and alleviating wrinkles through increasing collagen production, but also exhibits a strong whitening effect through inhibiting melanin production, and thus can be used as a composition for improving and whitening skin wrinkles.

[0052] A cosmetic composition comprising a fermented extract of Chajigi according to one embodiment of the present invention as an active ingredient is a natural ingredient that is harmless to the human body and exhibits a remarkable effect of increasing collagen production and inhibiting melanin production, and thus can be usefully used for purposes such as improving skin elasticity, improving skin wrinkles, and whitening skin, and thus has industrial applicability.

Claims

1. A cosmetic composition for improving skin elasticity, improving skin wrinkles, and whitening skin, containing a fermented extract of Perilla frutescensvar. acutakudo as an active ingredient.

2. In paragraph 1, A cosmetic composition, characterized in that the above fermented extract is extracted from at least one part selected from the group consisting of leaves, stems, flowers, fruits, and whole plants of Chazuki.

3. In paragraph 1, A cosmetic composition characterized in that the above fermented extract is extracted using water, an organic solvent, or a mixture thereof as a solvent and then fermented using a fermentation strain.

4. In paragraph 3, A cosmetic composition, characterized in that the fermentation strain is Bacillus subtilis.

5. In paragraph 3, A cosmetic composition, characterized in that the above fermentation is performed at 5°C to 80°C for 30 minutes to 10 days.

6. In paragraph 1, A cosmetic composition characterized in that the composition exhibits collagen production increasing efficacy and melanin production inhibition efficacy.

7. In paragraph 1, A cosmetic composition characterized in that the composition is in any one formulation selected from among a suspension, a solution, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleansing, an oil, a powder foundation, an emulsion foundation, a wax foundation, a pack, a massage cream, and a spray.

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