Control system for degrading vomitoxin using bacillus subtilis

Abstract

The application discloses a kind of to adopt bacillus subtilis degradation vomit toxin management and control system, belong to wisdom industry technical field, the management and control system is recorded in historical data by degradation reaction in different stages corresponding degradation condition and degradation result is analyzed, and according to the degradation reaction condition being carried out currently, the degradation result of future period is predicted, to facilitate the planned degradation work, in addition, for a degradation reaction tank, the actual degradation efficiency in a period of time is compared with the predicted degradation efficiency by the application, the abnormal degradation process can be found in time, and the staff is reminded to carry out timely targeted inspection, avoid inefficient degradation in a long period of time in degradation reaction tank or sensor damage and other negative effects on the monitoring and degradation process.

Description

Technical Field

[0001] This invention belongs to the field of smart industrial technology, specifically, it relates to a control system that uses Bacillus subtilis to degrade vomitoxin. Background Technology

[0002] Vomitoxin, named for its ability to induce vomiting in pigs, has caused severe harm to traditional animal husbandry and human production and daily life. Therefore, since its discovery in the last century, scientists have continuously researched methods for preventing, detoxifying, and eliminating this toxin. Currently known methods for removing vomitoxin from feed ingredients include physical, chemical, and biological methods. However, physical and chemical detoxification methods are not practically applicable to the current feed and livestock industries. These methods are difficult to implement, cannot handle large quantities of feed and raw materials, and often reduce the nutritional quality and palatability of feed after chemical detoxification. Currently, there is no truly effective method to prevent mycotoxin contamination in feed. Microorganisms or biological enzymes have become a focus of attention due to their accuracy, effectiveness, and environmental friendliness.

[0003] Bacillus subtilis is effective in decomposing vomitoxin and is a commonly used biological method for removing vomitoxin. However, during the decomposition of vomitoxin in feed by Bacillus subtilis, different conditions can affect the final degradation efficiency. When abnormalities occur during the degradation process, workers may not be able to detect them in time, which prolongs the entire degradation process and is not conducive to improving the degradation efficiency. In order to solve the above problems, the present invention provides the following technical solution. Summary of the Invention

[0004] The purpose of this invention is to provide a control system for degrading vomitoxin using Bacillus subtilis, which solves the problem in the prior art that workers cannot detect abnormal degradation in time during the degradation of vomitoxin in feed, which may lead to a prolonged degradation process and is not conducive to improving degradation efficiency.

[0005] The objective of this invention can be achieved through the following technical solutions:

[0006] A control system employing Bacillus subtilis to degrade vomitoxin includes:

[0007] Degradation reaction vessel, used to provide a place for degrading vomitoxin in the raw materials of the reaction;

[0008] The temperature detection unit is used to detect the reaction temperature in the degradation reaction vessel and input the results into the data storage unit for storage.

[0009] The pH detection unit is used to measure the pH value in the degradation reaction tank and input the results into the data storage unit for storage.

[0010] The biological detection unit is used to detect the concentration of vomitoxin in the reaction raw materials and input the results into the data storage unit for storage;

[0011] The controller is used to predict the degradation efficiency of the corresponding degradation reaction tank in the future based on the real-time calibration data set corresponding to the degradation reaction tank and the historical calibration data set stored in the data storage unit, and to identify abnormal degradation processes based on the predicted degradation efficiency and the actual degradation efficiency.

[0012] The alarm unit is used to issue alarm information to alert staff that there is an abnormality in the degradation process within the corresponding degradation reaction tank.

[0013] As a further aspect of the present invention, the method by which the controller predicts the degradation efficiency within the degradation reaction tank includes the following steps:

[0014] S1. Add the reaction raw materials and the Bacillus subtilis agent in a preset ratio to the degradation reaction tank, and then adjust the temperature and pH value in the degradation reaction tank to the preset range.

[0015] During the feeding process, the average concentration of vomitoxin in the reaction raw materials is collected by a biological detection unit;

[0016] During the reaction, the reaction temperature is monitored by a temperature detection unit, and the pH of the reaction in the degradation reaction tank is monitored by a pH detection unit.

[0017] S2. For a degradation reaction tank, the concentration of vomitoxin in the material inside the degradation reaction tank is collected by the biological detection unit every preset time t1. The obtained vomitoxin concentrations are sequentially marked as r1, r2, ..., rn, where n is the number of vomitoxin concentration data collected, and r1 is the vomitoxin concentration in the material inside the degradation reaction tank at the start of degradation.

[0018] The time interval t1 between the two collections of vomitoxin concentration was marked as the degradation sub-period;

[0019] The controller calculates the degradation efficiency Xi of vomitoxin in the degradation reaction tank based on the concentration ri of vomitoxin in the material at the beginning of a degradation sub-period and the concentration r(i+1) of vomitoxin in the material at the end of the degradation sub-period. Specifically, Xi = [r(i+1) - ri] / t1, where 1 ≤ i ≤ n-1.

[0020] Obtain the average pH value A and average reaction temperature T corresponding to each degradation sub-period;

[0021] The initial vomitoxin concentration rc, degradation efficiency Xi, initial pH value A, and initial reaction temperature T corresponding to a degradation period are used as a set of calibration data.

[0022] Obtain the calibration data set for each degradation sub-period corresponding to each degradation reaction vessel;

[0023] S4. While a degradation reaction is in progress in a degradation reaction tank, the concentration of vomitoxin in the material inside the degradation reaction tank is obtained by collecting samples once every preset time t1.

[0024] At the beginning of a degradation sub-period, the initial actual concentration of vomitoxin rs in the corresponding degradation reaction tank is obtained, as well as the real-time pH value As and real-time temperature Ts of the corresponding degradation reaction tank are obtained, and the degradation sub-period is marked as the sensing sub-period.

[0025] Obtain all calibration data sets where the initial vomitoxin concentration rc is equal to rs or satisfies |rc-rs| / rs≤θ1. Then, select from these calibration data sets where the initial pH value A is equal to the real-time pH value As or satisfies |A-As| / As≤θ1 and the initial reaction temperature T is equal to the real-time temperature Ts or satisfies |T-Ts| / Ts≤θ1. Mark these selected calibration data sets as control data sets.

[0026] Where θ1 is a preset parameter value;

[0027] The reference degradation efficiency Xc of the corresponding degradation reaction vessel during the sensing sub-period was calculated based on the vomitoxin concentration of each control data group.

[0028] As a further aspect of the present invention, the method for the biological detection unit to collect the average concentration of vomitoxin in the reaction raw materials includes the following steps:

[0029] During the feeding process, samples are collected sequentially at preset time intervals (ty) and the concentration of vomitoxin is detected. After deleting abnormal data, the average value of the remaining data is calculated as the average concentration of vomitoxin in the corresponding degradation reaction tank.

[0030] As a further aspect of the present invention, the method for calculating the reference degradation efficiency Xc of the degradation reaction vessel during the sensing sub-period is as follows:

[0031] Obtain the degradation efficiency corresponding to each control data group corresponding to the degradation reaction vessel, and label these degradation efficiencies as Xaj, where 1≤j≤m, and m is the number of control data groups corresponding to the corresponding degradation reaction vessel.

[0032] According to the formula The corresponding dispersion value D is calculated. When D≤D1, the reference degradation efficiency Xc of the corresponding degradation reaction vessel in the sensing sub-period is considered to be Xap.

[0033] When D > D1 holds true, the reference degradation efficiency Xc of the corresponding degradation reaction vessel in the sensing sub-period is considered to be the median of the corresponding m degradation efficiencies.

[0034] in D1 is the default value.

[0035] As a further aspect of the present invention, the method by which the controller identifies abnormal degradation processes is as follows: the predicted vomitoxin concentration ry of the corresponding degradation reaction tank at the end of the sensing sub-period is calculated based on the reference degradation efficiency Xc calculated at the beginning of the sensing sub-period and the initial actual vomitoxin concentration rs.

[0036] At the end of the sensing sub-period, the concentration of vomitoxin rs1 in the corresponding degradation reaction vessel is detected by the biodetection unit.

[0037] When θ3≥|rs1-ry| / ry≥θ2 holds true, it is considered that the degradation process of the corresponding degradation reaction vessel during the sensing sub-period is suspected of being abnormal.

[0038] If a degradation reactor shows β consecutive suspected abnormalities, the degradation process of the corresponding degradation reactor is considered to be abnormal; where θ2 and θ3 are preset values.

[0039] For a degradation reaction vessel, if |rs1-ry| / ry≥θ3 is satisfied, then the degradation process of the corresponding degradation reaction vessel is considered to be abnormal during the sensing sub-period.

[0040] When an abnormality is detected in the degradation process within a degradation reaction vessel, the alarm unit issues an alarm message, prompting the relevant personnel to inspect the corresponding degradation reaction vessel.

[0041] The beneficial effects of this invention are:

[0042] This invention analyzes the degradation conditions and results at different stages of the degradation reaction recorded in historical data, and predicts the degradation results for a future period based on the current degradation reaction conditions. This facilitates the planned implementation of degradation work. In addition, for a degradation reaction vessel, this invention compares the actual degradation efficiency with the predicted degradation efficiency over a period of time, enabling timely detection of abnormal degradation processes and alerting staff to conduct timely and targeted inspections. This avoids negative impacts on the monitoring and execution of the entire degradation process caused by inefficient degradation or sensor damage in the degradation reaction vessel over a long period. Attached Figure Description

[0043] The invention will now be further described with reference to the accompanying drawings.

[0044] Figure 1 This is a schematic diagram of the framework structure of the control system for degrading vomitoxin using Bacillus subtilis in this invention. Detailed Implementation

[0045] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0046] A control system using Bacillus subtilis to degrade vomitoxin, such as Figure 1 As shown, it includes:

[0047] Degradation reaction vessel, used to provide a place for degrading vomitoxin in the raw materials of the reaction;

[0048] The temperature detection unit is used to detect the reaction temperature in the degradation reaction vessel and input the results into the data storage unit for storage.

[0049] The pH detection unit is used to measure the pH value in the degradation reaction tank and input the results into the data storage unit for storage.

[0050] The biological detection unit is used to detect the concentration of vomitoxin in the reaction raw materials and input the results into the data storage unit for storage;

[0051] The reaction raw material is feed contaminated with vomitoxin;

[0052] The controller is used to predict the degradation efficiency of the corresponding degradation reaction tank in the future based on the real-time calibration data set corresponding to the degradation reaction tank and the historical calibration data set stored in the data storage unit, and to identify abnormal degradation processes based on the predicted degradation efficiency and the actual degradation efficiency.

[0053] The alarm unit is used to issue alarm information to alert staff that there is an abnormality in the degradation process inside the corresponding degradation reaction tank.

[0054] Includes the following steps:

[0055] S1. Add the reaction raw materials and the Bacillus subtilis agent in a preset ratio to the degradation reaction tank, and then adjust the temperature and pH value in the degradation reaction tank to the preset range.

[0056] During the feeding process, the average concentration of vomitoxin in the reaction raw materials is collected by a biological detection unit;

[0057] During the reaction, the reaction temperature is detected by the temperature detection unit and the reaction temperature data is transmitted to the data storage unit for storage. The reaction pH in the degradation reaction tank is detected by the pH detection unit and the reaction pH data is transmitted to the data storage unit for storage.

[0058] In one embodiment of the present invention, the method for collecting the average concentration of vomitoxin in the reaction raw materials by the biodetection unit includes the following steps:

[0059] During the feeding process, samples are collected sequentially at preset time intervals (ty) and the concentration of vomitoxin is detected. After deleting abnormally large or small data, the average value of the remaining data is calculated as the average concentration of vomitoxin in the corresponding degradation reaction tank.

[0060] Due to differences in feed types and varying vomitoxin concentrations at different locations within the same batch of feed, if only one sample is taken when continuously adding reaction materials to the degradation reaction tank via conveyor belt, the actual vomitoxin concentration in the degradation reaction tank will have a large error compared to the detected vomitoxin concentration.

[0061] S2. For a degradation reaction tank, the concentration of vomitoxin in the material inside the degradation reaction tank is collected by the biological detection unit every preset time t1. The obtained vomitoxin concentrations are sequentially marked as r1, r2, ..., rn, where n is the number of vomitoxin concentration data collected, and r1 is the vomitoxin concentration in the material inside the degradation reaction tank at the start of degradation.

[0062] The time interval t1 between the two collections of vomitoxin concentration was marked as the degradation sub-period;

[0063] The degradation efficiency Xi of vomitoxin in the degradation reaction tank is calculated based on the concentration ri of vomitoxin in the material at the beginning of a degradation sub-period and the concentration r(i+1) of vomitoxin in the material at the end of the degradation sub-period. Specifically, Xi = [r(i+1) - ri] / t1, where 1 ≤ i ≤ n-1.

[0064] Obtain the average pH value A and average reaction temperature T corresponding to each degradation sub-period;

[0065] The initial vomitoxin concentration rc (i.e., the concentration of vomitoxin in the material in the degradation reaction tank at the beginning of the degradation sub-period), degradation efficiency Xi, initial pH value A, and initial reaction temperature T corresponding to a degradation period are used as a set of calibration data.

[0066] Obtain the calibration data set for each degradation sub-period corresponding to each degradation reaction vessel;

[0067] The initial pH value A refers to the pH value inside the corresponding degradation reaction tank at the beginning of the corresponding degradation period.

[0068] The initial reaction temperature T refers to the temperature value inside the corresponding degradation reaction tank at the beginning of the corresponding degradation period.

[0069] S4. While a degradation reaction is in progress in a degradation reaction tank, the concentration of vomitoxin in the material inside the degradation reaction tank is obtained by collecting samples once every preset time t1.

[0070] At the beginning of a degradation sub-period, the initial actual concentration of vomitoxin rs in the corresponding degradation reaction tank is obtained, as well as the real-time pH value As and real-time temperature Ts of the corresponding degradation reaction tank are obtained, and the degradation sub-period is marked as the sensing sub-period.

[0071] Obtain all calibration data sets where the initial vomitoxin concentration rc is equal to rs or satisfies |rc-rs| / rs≤θ1. Then, select from these calibration data sets where the initial pH value A is equal to the real-time pH value As or satisfies |A-As| / As≤θ1 and the initial reaction temperature T is equal to the real-time temperature Ts or satisfies |T-Ts| / Ts≤θ1. Mark these selected calibration data sets as control data sets.

[0072] Where θ1 is a preset parameter value;

[0073] Obtain the reference degradation efficiency Xc of the corresponding degradation reaction vessel during the sensing sub-period;

[0074] The method for calculating the reference degradation efficiency Xc of the degradation reaction vessel during the sensing sub-period is as follows:

[0075] Obtain the degradation efficiency corresponding to each control data group corresponding to the degradation reaction vessel, and label these degradation efficiencies as Xaj, where 1≤j≤m, and m is the number of control data groups corresponding to the corresponding degradation reaction vessel.

[0076] According to the formula The corresponding dispersion value D is calculated. When D≤D1, the reference degradation efficiency Xc of the corresponding degradation reaction vessel in the sensing sub-period is considered to be Xap.

[0077] When D > D1 holds true, the reference degradation efficiency Xc of the corresponding degradation reaction vessel in the sensing sub-period is considered to be the median of the corresponding m degradation efficiencies.

[0078] in D1 is the default value;

[0079] S5. Based on the reference degradation efficiency Xc calculated at the beginning of the sensing sub-period and the initial actual vomitoxin concentration rs, the predicted vomitoxin concentration ry of the corresponding degradation reaction tank at the end of the sensing sub-period is calculated.

[0080] At the end of the sensing sub-period, the concentration of vomitoxin rs1 in the corresponding degradation reaction vessel is detected by the biodetection unit.

[0081] When θ3≥|rs1-ry| / ry≥θ2 holds true, it is considered that the degradation process of the corresponding degradation reaction vessel during the sensing sub-period is suspected of being abnormal.

[0082] If a degradation reactor shows β consecutive suspected abnormalities, then the degradation process of the corresponding degradation reactor is considered to be abnormal.

[0083] θ2 and θ3 are both preset values;

[0084] For a degradation reaction vessel, if |rs1-ry| / ry≥θ3 is satisfied, then the degradation process of the corresponding degradation reaction vessel is considered to be abnormal during the sensing sub-period.

[0085] S6. When an abnormality is detected in the degradation process within a degradation reaction tank, the alarm unit issues an alarm message, prompting the relevant personnel to inspect the corresponding degradation reaction tank and promptly identify and eliminate the degradation abnormality.

[0086] This invention analyzes the degradation conditions and results at different stages of the degradation reaction recorded in historical data, and predicts the degradation results for a future period based on the current degradation reaction conditions. This facilitates the planned implementation of degradation work. In addition, for a degradation reaction vessel, this invention compares the actual degradation efficiency with the predicted degradation efficiency over a period of time, enabling timely detection of abnormal degradation processes and alerting staff to conduct timely and targeted inspections. This avoids negative impacts on the monitoring and execution of the entire degradation process caused by inefficient degradation or sensor damage in the degradation reaction vessel over a long period.

[0087] In the description of this specification, the references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0088] The above description is merely an example and illustration of the present invention. Those skilled in the art can make various modifications or additions to the specific embodiments described, or use similar methods to replace them, as long as they do not deviate from the invention or exceed the scope defined in the claims, all of which should fall within the protection scope of the present invention.

Claims

1. A control system employing Bacillus subtilis to degrade vomitoxin, characterized in that, include: Degradation reaction vessel, used to provide a place for degrading vomitoxin in the raw materials of the reaction; The temperature detection unit is used to detect the reaction temperature in the degradation reaction vessel and input the results into the data storage unit for storage. The pH detection unit is used to measure the pH value in the degradation reaction tank and input the results into the data storage unit for storage. The biological detection unit is used to detect the concentration of vomitoxin in the reaction raw materials and input the results into the data storage unit for storage; The controller is used to predict the degradation efficiency of the corresponding degradation reaction tank in the future based on the real-time calibration data set corresponding to the degradation reaction tank and the historical calibration data set stored in the data storage unit, and to identify abnormal degradation processes based on the predicted degradation efficiency and the actual degradation efficiency. The alarm unit is used to issue alarm information to alert staff that there is an abnormality in the degradation process inside the corresponding degradation reaction tank. The method for calculating the reference degradation efficiency Xc of the degradation reaction vessel during the sensing sub-period is as follows: Obtain the degradation efficiency corresponding to each control data group corresponding to the degradation reaction vessel, and label these degradation efficiencies as Xaj, where 1≤j≤m, and m is the number of control data groups corresponding to the corresponding degradation reaction vessel. According to the formula The corresponding dispersion value D is calculated. When D≤D1, the reference degradation efficiency Xc of the corresponding degradation reaction vessel in the sensing sub-period is considered to be Xap. When D > D1 holds true, the reference degradation efficiency Xc of the corresponding degradation reaction vessel in the sensing sub-period is considered to be the median of the corresponding m degradation efficiencies. in D1 is the default value; The controller identifies abnormal degradation processes by calculating the predicted vomitoxin concentration ry of the corresponding degradation reaction tank at the end of the sensing sub-period based on the reference degradation efficiency Xc calculated at the beginning of the sensing sub-period and the initial actual vomitoxin concentration rs. At the end of the sensing sub-period, the concentration of vomitoxin rs1 in the corresponding degradation reaction vessel is detected by the biodetection unit. When θ3≥|rs1-ry| / ry≥θ2 holds true, it is considered that the degradation process of the corresponding degradation reaction vessel during the sensing sub-period is suspected of being abnormal. If a degradation reactor shows β consecutive suspected abnormalities, the degradation process of the corresponding degradation reactor is considered to be abnormal; where θ2 and θ3 are preset values. For a degradation reaction vessel, if |rs1-ry| / ry≥θ3 is satisfied, then the degradation process of the corresponding degradation reaction vessel is considered to be abnormal during the sensing sub-period. When an abnormality is detected in the degradation process within a degradation reaction vessel, the alarm unit issues an alarm message, prompting the relevant personnel to inspect the corresponding degradation reaction vessel.

2. The control system for degrading vomitoxin using Bacillus subtilis according to claim 1, characterized in that, The method for the controller to predict the degradation efficiency within the degradation reaction tank includes the following steps: S1. Add the reaction raw materials and the Bacillus subtilis agent in a preset ratio to the degradation reaction tank, and then adjust the temperature and pH value in the degradation reaction tank to the preset range. During the feeding process, the average concentration of vomitoxin in the reaction raw materials is collected by a biological detection unit; During the reaction, the reaction temperature is monitored by a temperature detection unit, and the pH of the reaction in the degradation reaction tank is monitored by a pH detection unit. S2. For a degradation reaction tank, the concentration of vomitoxin in the material inside the degradation reaction tank is collected by the biological detection unit every preset time t1. The obtained vomitoxin concentrations are sequentially marked as r1, r2, ..., rn, where n is the number of vomitoxin concentration data collected, and r1 is the vomitoxin concentration in the material inside the degradation reaction tank at the start of degradation. The time interval t1 between the two collections of vomitoxin concentration was marked as the degradation sub-period; The controller calculates the degradation efficiency Xi of vomitoxin in the degradation reaction tank based on the concentration ri of vomitoxin in the material at the beginning of a degradation sub-period and the concentration r(i+1) of vomitoxin in the material at the end of the degradation sub-period. Specifically, Xi = [r(i+1) - ri] / t1, where 1 ≤ i ≤ n-1. Obtain the average pH value A and average reaction temperature T corresponding to each degradation sub-period; The initial vomitoxin concentration rc, degradation efficiency Xi, initial pH value A, and initial reaction temperature T corresponding to a degradation period are used as a set of calibration data. Obtain the calibration data set for each degradation sub-period corresponding to each degradation reaction vessel; S4. While a degradation reaction is in progress in a degradation reaction tank, the concentration of vomitoxin in the material inside the degradation reaction tank is obtained by collecting samples once every preset time t1. At the beginning of a degradation sub-period, the initial actual concentration of vomitoxin rs in the corresponding degradation reaction tank is obtained, as well as the real-time pH value As and real-time temperature Ts of the corresponding degradation reaction tank are obtained, and the degradation sub-period is marked as the sensing sub-period. Obtain all calibration data sets where the initial vomitoxin concentration rc is equal to rs or satisfies |rc-rs| / rs≤θ1. Then, select from these calibration data sets where the initial pH value A is equal to the real-time pH value As or satisfies |A-As| / As≤θ1 and the initial reaction temperature T is equal to the real-time temperature Ts or satisfies |T-Ts| / Ts≤θ1. Mark these selected calibration data sets as control data sets. Where θ1 is a preset parameter value; The reference degradation efficiency Xc of the corresponding degradation reaction vessel during the sensing sub-period was calculated based on the vomitoxin concentration of each control data group.

3. The control system for degrading vomitoxin using Bacillus subtilis according to claim 2, characterized in that, The method for collecting the average vomitoxin concentration of the reaction raw materials by the biodetection unit includes the following steps: During the feeding process, samples are collected sequentially at preset time intervals (ty) and the concentration of vomitoxin is detected. After deleting abnormal data, the average value of the remaining data is calculated as the average concentration of vomitoxin in the corresponding degradation reaction tank.

Images