Sealed material removal device

Abstract

The application discloses a sealed material taking device and relates to the technical field of material taking. The device comprises a sealed tank for a sealed material taking environment; a plurality of storage cylinders arranged in the sealed tank; a support plate fixedly arranged in the sealed tank, wherein the plurality of storage cylinders are fixedly arranged on the support plate; a weighing table rotatably arranged on the inner bottom side of the sealed tank; and a material taking cup arranged on the weighing table and capable of receiving raw materials in the storage cylinders and weighing the raw materials through the weighing table. The sealed tank is used to assist in weighing the raw materials, so that the raw materials can be weighed quickly and accurately, and the direct contact with the outside is reduced in the sealed environment of the sealed tank.

Description

Technical Field

[0001] This application relates to the field of material handling technology, and more specifically, to a sealed material handling device. Background Technology

[0002] When preparing solid culture medium, it is necessary to calculate and weigh the various components according to the formula, dissolve the weighed components in water, add a coagulant and adjust the pH, and finally sterilize and pour the mixture into plates to obtain solid culture medium, which is widely used in the preparation of solid culture medium.

[0003] Although the existing technical solutions mentioned above can achieve the effect of preparing solid culture medium by dissolving each component in a coagulant, they still have the following drawbacks: when weighing each component, it is generally required to act quickly, because some components may be prone to absorbing moisture, increasing weighing errors and adversely affecting the cell culture effect of the solid culture medium in the later stage.

[0004] In view of this, we propose a sealed material handling device. Summary of the Invention

[0005] 1. Technical problems to be solved

[0006] The purpose of this application is to provide a sealed material handling device, which solves the technical problem of increased component content error during the weighing process and achieves the technical effect of rapid weighing of powder raw materials.

[0007] 2. Technical Solution

[0008] The present application relates to an improved cell culture medium, comprising a solid culture medium, wherein the solid culture medium is composed of several different types of substrate layers, the substrate layers being stored inside a substrate mold, and the substrate layers including a sugar substrate layer, an inorganic salt substrate layer, and a trace element substrate layer.

[0009] By adopting the above technical solution, a solid culture medium is formed by several substrate layers. Each substrate layer is less affected by air. When weighing, only the cutting size needs to be paid attention to. The content of the required components can be obtained according to the component content per square centimeter of the substrate layer. In addition, each substrate layer is easy to preserve and convenient for subsequent cell culture.

[0010] As an optional solution to the technical solution of this application, the sugar base layer includes a sugar solution with a mass content of 90%-95% and a coagulant of 5%-10%. The sugar solution and the coagulant are mixed and then solidified in the base mold. Specifically, the mass ratio of the sugar solution to the coagulant is 9:1.

[0011] The inorganic salt base layer comprises 50%-60% distilled water, 20%-30% inorganic salt, and 5%-10% coagulant by mass. The distilled water, inorganic salt, and coagulant are mixed and then solidified in the base mold. The inorganic salt is a type of salt that is easily soluble in water. Specifically, the mass ratio of inorganic salt to distilled water is 1:2, and the mass ratio of inorganic salt to coagulant is 3:1. The coagulant is agar.

[0012] The trace element base layer comprises a trace element solution with a mass content of 90%-95% and a coagulant of 5%-10%. The trace element solution and the coagulant are solidified in the base mold. Specifically, the mass ratio of the trace element solution to the coagulant is 9:1.

[0013] The coagulant is agar.

[0014] By adopting the above technical solution, sugars are mixed in a coagulant, which solidifies the sugars to form a standard model in the base material mold, facilitating later cutting and reducing the impact of air on the quality and grade of the sugars. Inorganic salts are first dissolved in distilled water, and then the coagulant is mixed with distilled water and heated. After the coagulant and distilled water are mixed, the water is distilled to separate most of the water, and a small amount of distilled water is mixed with inorganic salts and then mixed with the coagulant. After the coagulant solidifies, the inorganic salts are sealed inside, which can be used later to prepare solid culture media. Trace element solutions are mixed with the coagulant, and after the coagulant solidifies, the trace element solutions are sealed inside. When cutting the coagulant, the corresponding trace elements can be directly obtained, which is convenient for the use of trace elements. The sugar solution and trace element solution have better dispersibility with the coagulant, which is conducive to the uniform mixing of the two.

[0015] This application also provides the above-mentioned sealing and material handling device, including the following steps:

[0016] S1. Preparation of solid culture medium: First, quickly weigh any mass of sugar solution and place it in a container. Then, weigh a certain amount of agar according to the mass of the sugar solution. The mass ratio of sugar solution to agar is 9:1. After heating, raise the temperature to 98.5-99.5℃ and stir continuously to prevent the agar from settling to the bottom during the solid-liquid conversion process. Then, pour the mixed solution into the base material mold, sterilize it, and then cool and solidify it to obtain the sugar base material layer.

[0017] S2. Quickly weigh any mass of inorganic salt, which should be easily soluble in water, and place it in a container. Weigh a certain amount of distilled water according to the mass of the inorganic salt, with a mass ratio of inorganic salt to distilled water of 1:2. Weigh a certain amount of agar according to the mass of the inorganic salt, with a mass ratio of inorganic salt to agar of 3:1. After heating, raise the temperature to 98.5-99.5℃ and stir continuously. When the agar is completely converted into a liquid state, raise the temperature to 100-102℃ and distill the distilled water again to separate it. Then pour the mixed solution into another base material mold, sterilize it, and then cool and solidify it to obtain the inorganic salt base material layer.

[0018] S3. Finally, quickly weigh any mass of trace element solution. The solution can be selected according to the requirements of the culture medium. Weigh a certain amount of agar according to the mass of the trace element solution. The mass ratio of trace element solution to agar is 9:1. After heating, raise the temperature to 98.5-99.5℃ and stir continuously. Then pour the mixed solution into the base material mold, sterilize it, and then cool and solidify it to obtain the trace element base material layer.

[0019] S4. Using a 1cm*1cm die cutter, cut out the sugar base layer, inorganic salt base layer and trace element base layer from the inside of the base material mold, and test the sugar content, inorganic salt content and trace element content. After testing, affix a label to the outside of the base material mold, and finally store each base material mold in a sterile environment at 0-3℃ for later use.

[0020] S5. When culturing solid culture medium, first cut different sized substrate blocks according to the relative contents of the sugar substrate layer, inorganic salt substrate layer, and trace element substrate layer. Place the various substrate blocks in a beaker and liquefy each substrate block by heating the beaker at a temperature between 98-100℃, while stirring constantly to prevent settling and to ensure that all components are mixed evenly. Stop heating when all the agar has been converted into a liquid state, and place the beaker in a sterile environment to cool naturally. When the temperature drops to 65-75℃, add other components inside.

[0021] S6. Remove the solid culture medium from the beaker, cut it and place it in a petri dish, then add other solid components according to the cell culture conditions.

[0022] By adopting the above technical solution, the pre-prepared sugar substrate layer, inorganic salt substrate layer and trace element substrate layer are easy to preserve, which brings convenience to subsequent cell culture. In addition, the requirements for weighing raw materials during the preparation of each substrate layer are low, and the content values ​​measured afterward are less affected by external interference during subsequent use, thus reducing weighing errors.

[0023] To prevent moisture absorption during the preparation of this improved cell culture medium, a sealed feeding device is used, which includes a sealed container for sealing the feeding environment.

[0024] Storage cylinders are disposed in the sealed container, and multiple storage cylinders are provided;

[0025] A support plate is fixedly installed in the sealed container, and multiple storage cylinders are also fixedly installed on the support plate;

[0026] A weighing platform is rotatably mounted on the bottom inside the sealed container;

[0027] The material receiving cup, set on the supporting platform, is capable of receiving the raw materials in the storage cylinder and weighing the raw materials through the weighing platform.

[0028] As an optional solution to the technical solution of this application, the storage cylinder includes several powder cylinders for storing powder raw materials and water cylinders for storing distilled water. A support plate is fixedly installed on the top of the support plate, and a drive gear is rotatably installed on the top of the support plate. The drive gear drives the powder cylinders to feed under the drive of the drive assembly through the transmission assembly A. A disc is fixedly installed at the bottom of the sealed tank, and the weighing platform is rotatably installed on the outside of the disc through a slip ring. The drive assembly drives the weighing platform to rotate intermittently through the transmission assembly B.

[0029] By adopting the above technical solution, when weighing raw materials, the feeding cup is placed on top of the weighing platform. The weighing platform is driven to rotate along the slip ring, causing the feeding cup to pass under the powder cylinder in sequence. After the weighing platform rotates to a certain angle, the transmission component B is disconnected from the drive component. Then, the drive component continues to drive the corresponding powder cylinder to feed through the transmission component A. When the feeding cup is at the origin, the receiving of all raw materials is completed. During the receiving process, the weight of the feeding cup is detected by the weighing platform. The change in weight each time is equal to the required amount of the corresponding raw material, making the weighing of raw materials faster and more accurate. At the same time, the sealed environment of the sealed container reduces direct contact with the outside world.

[0030] As an optional solution to the technical solution of this application, a base is fixedly provided at the bottom of the sealed tank, a pick-up and put-out port for picking up the material cup is provided on the outside of the sealed tank, a sealing door for sealing the sealed tank is rotatably provided on the inside of the pick-up and put-out port, a display controller for controlling the operation of the device is fixedly provided on one side of the base, a sealing cover A is fixedly provided on the top of the sealed tank, and a bearing plate for assembling the drive assembly is fixedly provided on the inside of the sealed tank.

[0031] By adopting the above technical solution, after the power is turned on, the required amount of each raw material is set sequentially through the display and controller. When the mass detected by the weighing platform reaches the required amount, the display and controller controls the device to weigh the next raw material, and so on, so that the device can automatically complete the material picking work. When it is necessary to replenish raw materials to the powder cylinder and water cylinder, the sealing cover A on the top of the sealed tank can be removed.

[0032] As an optional solution to the technical solution of this application, a discharge pipe is fixedly provided at the bottom of the powder cylinder, a rotating shaft is rotatably provided on the inner side of the powder cylinder via a bearing bracket, a screw feeder is fixedly provided on the outer side of the rotating shaft located inside the discharge pipe, a stirring rod is fixedly provided on the outer side of the rotating shaft located inside the powder cylinder, a sealing cover B is fixedly provided at the top of the powder cylinder, a sealing cap is fixedly provided on the top of the sealing cover B, and a worm gear is fixedly provided on the top of the rotating shaft located outside the sealing cover B. The drive assembly drives the worm gear to rotate through the transmission assembly A.

[0033] By adopting the above technical solution, when the drive component drives the worm gear to rotate, the worm gear drives the rotating shaft to rotate inside the powder cylinder, which in turn drives the screw feeder to rotate inside the discharge pipe. The screw feeder evenly outputs the raw material inside the powder cylinder. At the same time, the rotating shaft drives the stirring rod to rotate inside the powder cylinder, preventing the powder raw material inside the powder cylinder from accumulating and blocking, and ensuring the smoothness of the feeding. The sealing cap located on the top of the sealing cover B is used to directly open and add material into the powder cylinder when replenishing raw materials.

[0034] As an optional solution to the technical solution of this application, the driving component includes a motor, which is fixedly mounted on the top of the support plate. Both output ends of the motor are fixedly mounted with drive shafts. The top of the drive shaft is fixedly mounted with a drive gear, and the bottom end of the drive shaft drives the weighing platform to slide through the transmission component B.

[0035] By adopting the above technical solution, the drive shaft at the output end of the motor is rotated, which in turn drives the active gear to rotate on the top of the support plate. The active gear drives the powder cylinder to feed the powder through the transmission component A. At the same time, the drive shaft drives the weighing platform to slide intermittently through the transmission component B, which is used to weigh each raw material in sequence.

[0036] As an optional solution to the technical solution of this application, the support plate is fixedly mounted on the top of the support plate by a fixing plate. Support arms are evenly fixedly mounted on the outer side of the support plate. The transmission component A includes a driven gear, which is rotatably mounted on the top of the support arm. A bevel gear A is rotatably mounted on the bottom of the support arm and coaxially with the driven gear. A bevel gear B is meshed on the outer side of the bevel gear A. The bevel gear B is rotatably mounted on the outer side of the U-shaped seat via a connecting shaft. A worm gear is rotatably mounted on the inner side of the U-shaped seat via a connecting shaft. The worm gear meshes with the worm wheel in the powder cylinder. A connecting plate is fixedly mounted on the top of the U-shaped seat. The other end of the connecting plate is rotatably mounted on the support arm. An electric push rod A is rotatably mounted on the outer side of the connecting plate. The other end of the electric push rod A is rotatably mounted on the bottom of the support arm.

[0037] By adopting the above technical solution, when the driving gear rotates, it drives the driven gear in the transmission assembly A to rotate, which in turn drives the bevel gear A to rotate. When the bevel gear A rotates, it drives the worm gear on the inner side of the U-shaped seat to rotate through the bevel gear B meshing on the outer side. This causes the worm gear to drive the worm wheel in the powder cylinder, thus driving the powder cylinder. When the powder cylinder reaches the preset amount, the electric push rod A on the outer side of the connecting plate pulls the U-shaped seat away from the worm wheel, causing the worm gear to disengage from the worm wheel and disengage the transmission. At this time, the powder cylinder stops feeding. When the weighing platform is below the next powder cylinder, the electric push rod A pushes the U-shaped seat in the next transmission assembly A towards the worm wheel, so that the driving gear can cooperate with multiple powder cylinders to perform feeding work during continuous rotation.

[0038] As an optional solution to the technical solution of this application, the transmission component B includes a slide rail, which is fixedly disposed on the outside of the weighing platform. Toothed plates are symmetrically slidably disposed on the outside of the slide rail. An electric push rod B is fixedly disposed between the toothed plates. Engaging gears are meshed between the symmetrically disposed toothed plates. The engaging gears rotate under the drive of the drive component.

[0039] By adopting the above technical solution, when the drive component drives the engagement gear to rotate, the engagement gear drives the weighing platform to rotate through the outer engagement tooth plate, causing the weighing platform to slide along the slip ring. When the material cup is directly below the powder cylinder, the electric push rod B pushes the tooth plates away from each other, causing the tooth plates to disengage from the engagement gear. At this time, the engagement gear continues to rotate under the drive of the drive component, and the material is ready to be weighed directly below the powder cylinder. After weighing, the tooth plate is engaged with the outer side of the engagement gear again through the electric push rod B. This process is repeated, so that the drive component drives the weighing platform to slide intermittently through the transmission component B.

[0040] As an optional solution to the technical solution of this application, a water valve is fixedly installed at the bottom of the water cylinder, a push rod for driving the water valve is slidably installed inside the water cylinder, an inclined pressure block A is fixedly installed at the top of the push rod, an inclined pressure block B is slidably installed on the inclined surface of the inclined pressure block A, a connecting arm is fixedly installed at the top of the inclined pressure block B, an electric push rod A is rotatably installed on the outside of the connecting arm, and the electric push rod A is rotatably installed with one of the support arms on the outside of the support plate.

[0041] By adopting the above technical solution, when weighing distilled water, the electric push rod A at the bottom of one of the support arms pushes the connecting arm to rotate, causing the connecting arm to drive the inclined pressure block B at the bottom to slide towards the top of the inclined pressure block A. Under the mutual compression of the inclined pressure block B and the inclined pressure block A, the inclined pressure block A pushes the push rod to slide inside the water cylinder, causing the push rod to drive the water valve to open and close, so that a certain amount of distilled water can be released and then closed in time.

[0042] 3. Beneficial effects

[0043] One or more technical solutions provided in the embodiments of this application have at least the following technical effects or advantages:

[0044] 1. This application uses a sealed container to assist in weighing each raw material, which makes the weighing of raw materials faster and more accurate, while reducing direct contact with the outside world in the sealed environment of the container.

[0045] 2. This application provides a powder cylinder for storing various powdered raw materials and a water cylinder for storing distilled water inside the sealed container. This allows the powder cylinder to be easily fed with powdered raw materials under the drive of the drive component. At the same time, the drive component can be selectively linked with several powder cylinders through the transmission component A, so that the powder cylinders can discharge raw materials sequentially.

[0046] 3. This application provides a material receiving cup located directly below the powder cylinder. The material receiving cup passes sequentially below the powder cylinder under the drive of the weighing platform. Under the action of the transmission component B, the weighing platform and the drive component maintain intermittent linkage, so that the drive component can complete the feeding and sliding of the weighing platform respectively when running continuously. Attached Figure Description

[0047] Figure 1 This is a schematic diagram of the structure of the substrate layer in the improved cell culture medium disclosed in a preferred embodiment of this application;

[0048] Figure 2 This is an isometric structural schematic diagram of a sealing material handling device disclosed in a preferred embodiment of this application;

[0049] Figure 3 This is a schematic diagram of the internal structure of the sealing material handling device disclosed in a preferred embodiment of this application;

[0050] Figure 4 This is a schematic diagram of the bottom structure inside the sealed material handling device disclosed in a preferred embodiment of this application;

[0051] Figure 5 for Figure 3 Schematic diagram of the structure at point A;

[0052] Figure 6 This is an exploded structural diagram of a sealing material handling device disclosed in a preferred embodiment of this application;

[0053] Figure 7 This is a schematic diagram of the support plate structure in a preferred embodiment of the sealing material handling device disclosed in this application;

[0054] Figure 8 This is a schematic diagram of the drive assembly in a preferred embodiment of the sealing material handling device disclosed in this application;

[0055] Figure 9This is a schematic diagram of the structure of the powder cylinder in the sealed material handling device disclosed in a preferred embodiment of this application;

[0056] Explanation of the labels in the diagram: 1. Base material layer; 2. Base material mold; 3. Sealed tank; 31. Support plate; 32. Bearing plate; 33. Base; 34. Loading / unloading port; 35. Sealed door; 36. Display and control unit; 37. Sealed cover A; 4. Powder cylinder; 41. Water cylinder; 411. Push rod; 412. Inclined pressure block A; 413. Inclined pressure block B; 414. Connecting arm; 415. Water valve; 42. Discharge pipe; 43. Sealed cover B; 44. Sealing cap; 45. Bearing bracket; 46. Rotating shaft; 47. Screw feeder; 48. Stirring rod; 49. 5. Worm gear; 51. Support plate; 52. Fixed plate; 53. Support arm; 54. Electric push rod A; 6. Drive gear; 7. Drive assembly; 71. Motor; 72. Drive shaft; 8. Transmission assembly A; 81. Driven gear; 82. Bevel gear A; 83. Bevel gear B; 84. Connecting shaft; 85. U-shaped seat; 86. Worm; 87. Connecting plate; 9. Weighing platform; 91. Picking cup; 92. Disc; 93. Slip ring; 94. Transmission assembly B; 941. Slide rail; 942. Gear plate; 943. Electric push rod B; 944. Engaging gear. Detailed Implementation

[0057] The present application will be further described in detail below with reference to the accompanying drawings.

[0058] Reference Figure 1 This application discloses an improved cell culture medium, including a solid culture medium. The solid culture medium is composed of several different types of substrate layers 1, which are respectively stored inside a substrate mold 2. The substrate layers 1 include a sugar substrate layer, an inorganic salt substrate layer, and a trace element substrate layer. The relative contents of the sugar substrate layer, the inorganic salt substrate layer, and the trace element substrate layer can be configured according to requirements.

[0059] The solid culture medium is composed of several substrate layers. Each substrate layer is less affected by air, and only the cutting size needs to be considered when weighing. The required component content can be obtained based on the component content per square centimeter of the substrate layer. Furthermore, each substrate layer is easy to store and convenient for subsequent cell culture.

[0060] Reference Figure 1 The sugar base layer comprises a sugar solution with a mass content of 90%-95% and a coagulant of 5%-10%. The sugar solution and coagulant are mixed and then solidified in the base mold 2. The sugar solution is preferably a 10% glucose solution, and the coagulant is preferably agar.

[0061] Sugars are mixed with a coagulant, which solidifies the sugars to form a standard model in the base mold 2, facilitating later cutting and reducing the impact of air on the quality and grade of the sugars.

[0062] Reference Figure 1 The inorganic salt base layer includes 50%-60% distilled water, 20%-30% inorganic salt and 5%-10% coagulant by mass. The distilled water, inorganic salt and coagulant are mixed and solidified in the base mold 2. The inorganic salt is preferably a salt that is easily soluble in water, and the coagulant is preferably agar.

[0063] The inorganic salts are first dissolved in distilled water, then the coagulant is mixed with distilled water and heated. After the coagulant and distilled water are mixed, the water is distilled to separate most of the water, and a small amount of distilled water is mixed with the inorganic salts and the coagulant. After the coagulant solidifies, the inorganic salts are sealed inside, so that they can be used later to prepare solid culture media.

[0064] Reference Figure 1 The trace element base layer includes a trace element solution with a mass content of 90%-95% and a coagulant of 5%-10%. The trace element solution and the coagulant are solidified in the base mold 2, wherein the coagulant is preferably agar.

[0065] The trace element solution is mixed with a coagulant. After the coagulant solidifies, the trace element solution is sealed inside. When the coagulant is cut, the corresponding trace element can be directly obtained, which facilitates the use of trace elements.

[0066] Reference Figure 1 A sealed material handling device includes the following steps:

[0067] S1. Preparation of solid culture medium: First, quickly weigh any mass of sugar solution and place it in a container. Then, weigh a certain amount of agar according to the mass of the sugar solution. The mass ratio of sugar solution to agar is 9:1. After heating, raise the temperature to 98.5-99.5℃ and stir continuously to prevent the agar from settling to the bottom during the solid-liquid conversion process. Then, pour the mixed solution into the base material mold 2, sterilize it, and then cool and solidify it to obtain the sugar base material layer.

[0068] S2. Quickly weigh any mass of inorganic salt, which should be easily soluble in water, and place it in a container. Weigh a certain amount of distilled water according to the mass of the inorganic salt, with a mass ratio of inorganic salt to distilled water of 1:2. Weigh a certain amount of agar according to the mass of the inorganic salt, with a mass ratio of inorganic salt to agar of 3:1. After heating, raise the temperature to 98.5-99.5℃ and stir continuously. When the agar is completely converted into a liquid state, raise the temperature to 100-102℃ and distill the distilled water again to separate it. Then pour the mixed solution into another base material mold 2, sterilize it, and then cool and solidify it to obtain the inorganic salt base material layer.

[0069] S3. Finally, quickly weigh any mass of trace element solution. The solution can be selected according to the requirements of the culture medium. Weigh a certain amount of agar according to the mass of the trace element solution. The mass ratio of trace element solution to agar is 9:1. After heating, raise the temperature to 98.5-99.5℃ and stir continuously. Then pour the mixed solution into the base material mold 2, sterilize it, and then cool and solidify it to obtain the trace element base material layer.

[0070] S4. Using a 1cm*1cm die cutter, cut out the sugar base layer, inorganic salt base layer and trace element base layer from the inside of the base material mold 2, and test the sugar content, inorganic salt content and trace element content. After testing, affix a label to the outside of the base material mold 2. Finally, store each base material mold 2 in a sterile environment at 0-3℃ for later use.

[0071] S5. When culturing solid culture medium, first cut different sized substrate blocks according to the relative contents of the sugar substrate layer, inorganic salt substrate layer, and trace element substrate layer. Place the various substrate blocks in a beaker and liquefy each substrate block by heating the beaker at a temperature between 98-100℃, while stirring constantly to prevent settling and to ensure that all components are mixed evenly. Stop heating when all the agar has been converted into a liquid state, and place the beaker in a sterile environment to cool naturally. When the temperature drops to 65-75℃, add other components inside.

[0072] S6. Remove the solid culture medium from the beaker, cut it and place it in a petri dish. Then add other solid components according to the cell culture conditions and carry out cell culture under the specified culture conditions.

[0073] The pre-prepared sugar substrate layer, inorganic salt substrate layer, and trace element substrate layer are easy to preserve, which facilitates subsequent cell culture. Furthermore, the requirements for weighing raw materials during the preparation of each substrate layer are relatively low, and the content values ​​measured after preparation are less affected by external interference during subsequent use, thus reducing weighing errors.

[0074] Reference Figure 2-6The raw material weighing in S1, S2 and S3 is accomplished by a material handling device, which includes a sealed container 3 for sealing the material handling environment. A support plate 31 is fixedly installed inside the sealed container 3. A storage cylinder is fixedly installed at the end of the support plate 31. The storage cylinder includes several powder cylinders 4 for storing powder raw materials and a water cylinder 41 for storing distilled water. A support plate 5 is fixedly installed on the top of the support plate 31. A drive gear 6 is rotatably installed on the top of the support plate 5. The drive gear 6 drives the powder cylinders 4 to feed material through the transmission component A8 under the drive component 7. A disc 92 is fixedly installed at the bottom of the sealed container 3. A weighing platform 9 for weighing is rotatably installed on the outside of the disc 92 through a slip ring 93. A material handling cup 91 for holding raw materials is installed on the top of the weighing platform 9. The drive component 7 drives the weighing platform 9 to rotate intermittently through the transmission component B94.

[0075] Sugar, inorganic salt, trace element, and agar raw material powders are separately placed in several powder cylinders 4, and distilled water is stored in a water cylinder 41. When weighing raw materials, the picking cup 91 is placed on top of the weighing platform 9. The weighing platform 9 is driven to rotate along the slip ring 93, so that the weighing platform 9 drives the picking cup 91 to pass directly below the powder cylinders 4 in sequence. After the weighing platform 9 has rotated a certain angle, the transmission component B94 disconnects the transmission from the drive component 7. Then, the drive component 7 continues to drive the corresponding powder cylinder 4 to discharge material through the transmission component A8. When the picking cup 91 is at the origin, all raw materials are received. During the receiving process, the weight of the picking cup 91 is detected by the weighing platform 9. The change in weight each time is equal to the required amount of the corresponding raw material, making the weighing of raw materials faster and more accurate. At the same time, the sealed environment of the sealed container 3 reduces direct contact with the outside world.

[0076] Reference Figure 6 The bottom of the sealed container 3 is fixedly provided with a base 33. The outer side of the sealed container 3 is provided with a pick-up and put-out port 34 for picking up the pick-up cup 91. The inner side of the pick-up and put-out port 34 is provided with a sealing door 35 for sealing the sealed container 3. A display controller 36 for controlling the operation of the device is fixedly provided on one side of the base 33. A sealing cover A37 is fixedly provided on the top of the sealed container 3. A bearing plate 32 for assembling the drive assembly 7 is fixedly provided on the inner side of the sealed container 3.

[0077] After the power is turned on, the required amount of each raw material is set sequentially through the display controller 36. When the mass detected by the weighing platform 9 reaches the required amount, the display controller 36 controls the device to weigh the next raw material, and so on, so that the device can automatically complete the material picking work. When it is necessary to replenish the raw materials to the powder cylinder 4 and the water cylinder 41, the sealing cover A37 on the top of the sealed tank 3 can be removed.

[0078] Reference Figure 9A discharge pipe 42 is fixedly installed at the bottom of the powder cylinder 4. A rotating shaft 46 is rotatably installed inside the powder cylinder 4 via a bearing bracket 45. A screw feeder 47 is fixedly installed on the outside of the rotating shaft 46 inside the discharge pipe 42. A stirring rod 48 is fixedly installed on the outside of the rotating shaft 46 inside the powder cylinder 4. A sealing cover B43 is fixedly installed at the top of the powder cylinder 4. A sealing cap 44 is fixedly installed on the top of the sealing cover B43. A worm gear 49 is fixedly installed on the top of the rotating shaft 46 outside the sealing cover B43. The drive assembly 7 drives the worm gear 49 to rotate through the transmission assembly A8.

[0079] When the drive assembly 7 drives the worm gear 49 to rotate, the worm gear 49 drives the rotating shaft 46 to rotate inside the powder cylinder 4, causing the rotating shaft 46 to drive the screw feeder 47 to rotate inside the discharge pipe 42. The screw feeder 47 evenly outputs the raw material inside the powder cylinder 4. At the same time, the rotating shaft 46 drives the stirring rod 48 to rotate inside the powder cylinder 4 to prevent the powder raw material inside the powder cylinder 4 from accumulating and blocking, ensuring the smoothness of the feeding. The sealing cap 44 located on the top of the sealing cover B43 is used to directly open and add material into the powder cylinder 4 when replenishing raw materials.

[0080] Reference Figure 8 The drive assembly 7 includes a motor 71, which is fixedly mounted on the top of the support plate 31. Both output ends of the motor 71 are fixedly mounted with drive shafts 72. The top of the drive shafts 72 is fixedly mounted with the drive gear 6. The bottom end of the drive shafts 72 drives the weighing platform 9 to slide through the transmission assembly B94.

[0081] The drive shaft 72 at the output end is driven by the motor 71 to rotate, which in turn drives the drive gear 6 to rotate on the top of the support plate 5. The drive gear 6 drives the powder cylinder 4 to feed the powder through the transmission assembly A8. At the same time, the drive shaft 72 drives the weighing platform 9 to slide intermittently through the transmission assembly B94, which is used to weigh each raw material in sequence.

[0082] Reference Figure 3 , Figure 5 and Figure 7The support plate 5 is fixedly mounted on the top of the support plate 31 by the fixing plate 51. Support arms 52 are evenly fixedly mounted on the outer side of the support plate 5. The transmission component A8 includes a driven gear 81, which is rotatably mounted on the top of the support arm 52. A bevel gear A82 is rotatably mounted on the bottom of the support arm 52 and coaxially with the driven gear 81. A bevel gear B83 is meshed on the outer side of the bevel gear A82. The bevel gear B83 is rotatably mounted on the outer side of the U-shaped seat 85 through the connecting shaft 84. A worm gear 86 is rotatably mounted on the inner side of the U-shaped seat 85 through the connecting shaft 84. The worm gear 86 meshes with the worm wheel 49 in the powder cylinder 4 for transmission. A connecting plate 87 is fixedly mounted on the top of the U-shaped seat 85. The other end of the connecting plate 87 is rotatably mounted on the support arm 52. An electric push rod A53 is rotatably mounted on the outer side of the connecting plate 87. The other end of the electric push rod A53 is rotatably mounted on the bottom of the support arm 52.

[0083] When the driving gear 6 rotates, it drives the driven gear 81 in the transmission assembly A8 to rotate, which in turn drives the bevel gear A82 to rotate. When the bevel gear A82 rotates, it drives the worm 86 on the inner side of the U-shaped seat 85 to rotate through the bevel gear B83 that is meshed on the outer side. This causes the worm 86 to drive the worm wheel 49 in the powder cylinder 4 to rotate, thus driving the powder cylinder 4. When the powder cylinder 4 reaches the preset amount of material, the electric push rod A53 on the outer side of the connecting plate 87 pulls the U-shaped seat 85 away from the worm wheel 49, causing the worm 86 to disengage from the worm wheel 49 and disengage the transmission. At this time, the powder cylinder 4 stops discharging. When the weighing platform 9 is below the next powder cylinder 4, the electric push rod A53 pushes the U-shaped seat 85 in the next transmission assembly A8 toward the worm wheel 49, so that the driving gear 6 can cooperate with multiple powder cylinders 4 to perform material discharging during continuous rotation.

[0084] Reference Figure 4 The transmission component B94 includes a slide rail 941, which is fixedly disposed on the outside of the weighing platform 9. Toothed plates 942 are symmetrically slidably disposed on the outside of the slide rail 941. An electric push rod B943 is fixedly disposed between the toothed plates 942. Engaging gears 944 are meshed between the symmetrically disposed toothed plates 942. The engaging gears 944 rotate under the drive of the drive component 7.

[0085] When the drive assembly 7 drives the engagement gear 944 to rotate, the engagement gear 944 drives the weighing platform 9 to rotate through the outer engagement toothed plate 942, causing the weighing platform 9 to slide along the slip ring 93. When the material cup 91 is directly below the powder cylinder 4, the electric push rod B943 pushes the toothed plate 942 away from each other, causing the toothed plate 942 to disengage from the engagement gear 944. At this time, the engagement gear 944 continues to rotate under the drive of the drive assembly 7, while 910 is located directly below the powder cylinder 4 to prepare to weigh the raw material. After weighing, the electric push rod B943 engages the toothed plate 942 with the outer engagement gear 944. This process is repeated, causing the drive assembly 7 to drive the weighing platform 9 to slide intermittently through the transmission assembly B94.

[0086] Reference Figure 3 and Figure 7 A water valve 415 is fixedly installed at the bottom of the water cylinder 41. A push rod 411 for driving the water valve 415 is slidably installed inside the water cylinder 41. An inclined pressure block A412 is fixedly installed at the top of the push rod 411. An inclined pressure block B413 is slidably installed on the inclined surface of the inclined pressure block A412. A connecting arm 414 is fixedly installed at the top of the inclined pressure block B413. An electric push rod A53 is rotatably installed on the outside of the connecting arm 414. The electric push rod A53 is rotatably installed with one of the support arms 52 on the outside of the support plate 5.

[0087] When weighing distilled water, the electric push rod A53 at the bottom of one of the support arms 52 pushes the connecting arm 414 to rotate, causing the connecting arm 414 to drive the inclined pressure block B413 at the bottom to slide towards the top of the inclined pressure block A412. Under the mutual compression of the inclined pressure block B413 and the inclined pressure block A412, the inclined pressure block A412 pushes the push rod 411 to slide inside the water cylinder 41, causing the push rod 411 to drive the water valve 415 to open and close, so that a certain amount of distilled water can be released and then closed in time.

[0088] Working principle: First, sugar, inorganic salt, trace element and agar raw material powder are placed in several powder cylinders 4 respectively, and distilled water is stored in water cylinder 41. After the power is turned on, the required amount of each raw material is set in sequence by display and controller 36. Display and controller 36 controls the drive component 7 inside the sealed tank 3 according to the preset value, so that the drive shaft 72 in drive component 7 drives the weighing platform 9 to slide intermittently through transmission component B94.

[0089] Specifically, the drive shaft 72 drives the engagement gear 944 in the transmission assembly B94 to rotate. The engagement gear 944 drives the weighing platform 9 to rotate through the outer engagement toothed plate 942, causing the weighing platform 9 to slide along the slip ring 93. When the material cup 91 is directly below the powder cylinder 4, the electric push rod B943 pushes the toothed plates 942 away from each other, causing the toothed plates 942 to disengage from the engagement gear 944. At this time, the engagement gear 944 continues to rotate under the drive of the drive assembly 7, and the drive assembly 7 drives the drive gear 6 to continue rotating. Meanwhile, 910 is located directly below the powder cylinder 4, ready to weigh the raw material. When the drive gear 6 rotates, it drives the driven gear 81 in the transmission assembly A8 to rotate, causing the driven gear 81 to drive the bevel gear A82 to rotate. When the bevel gear A82 rotates, it drives the worm 86 on the inner side of the U-shaped seat 85 to rotate through the outer meshing bevel gear B83, causing the worm 86 to drive the worm wheel 49 in the powder cylinder 4 to rotate, thus driving the powder cylinder 4.

[0090] When the powder cylinder 4 reaches the preset amount of material, the electric push rod A53 on the outside of the connecting plate 87 pulls the U-shaped seat 85 away from the worm wheel 49, causing the worm 86 to disengage from the worm wheel 49 and disengage the transmission. At this time, the powder cylinder 4 stops discharging material. When the weighing platform 9 is below the next powder cylinder 4, the electric push rod A53 pushes the U-shaped seat 85 in the next transmission assembly A8 toward the worm wheel 49, so that the drive gear 6 can cooperate with multiple powder cylinders 4 to perform material discharging work during continuous rotation.

[0091] When weighing distilled water, the electric push rod A53 at the bottom of one of the support arms 52 pushes the connecting arm 414 to rotate, causing the connecting arm 414 to drive the inclined pressure block B413 at the bottom to slide towards the top of the inclined pressure block A412. Under the mutual compression of the inclined pressure block B413 and the inclined pressure block A412, the inclined pressure block A412 pushes the push rod 411 to slide inside the water cylinder 41, causing the push rod 411 to drive the water valve 415 to open and close, so that a certain amount of distilled water can be released and then closed in time, thereby achieving the effect of quickly weighing raw materials.

[0092] After the materials are collected, the culture medium is prepared. Specifically, the mass ratio of sugar solution to agar is 9:1. The weighed raw materials are heated to 98.5-99.5℃ and stirred continuously to prevent the agar from settling to the bottom during the solid-liquid conversion process. The mixed solution is then poured into base mold 2, sterilized, and then cooled and solidified to obtain the sugar base layer. The mass ratio of inorganic salt to distilled water is 1:2. A certain amount of agar is weighed according to the mass of inorganic salt, with a mass ratio of inorganic salt to agar of 3:1. After weighing, the agar is heated to 98.5-99.5℃ and stirred continuously. When the agar is completely converted to liquid, the temperature is raised to 100-102℃, and the distilled water is distilled again to separate it. The mixed solution is then poured into another base mold 2, sterilized, and then cooled and solidified to obtain the inorganic salt base layer.

[0093] Finally, the mass ratio of trace element solution to agar is 9:1. After heating, the temperature is raised to 98.5-99.5℃ and stirred continuously. Then, the mixed solution is poured into the base material mold 2, sterilized, and then cooled and solidified to obtain the trace element base material layer.

[0094] Using a 1cm*1cm die cutter, sugar base material layer, inorganic salt base material layer and trace element base material layer are cut out from the inside of base material mold 2, and the sugar content, inorganic salt content and trace element content are tested. After testing, a label is affixed to the outside of base material mold 2. Finally, each base material mold 2 is stored in a sterile environment at 0-3℃ for later use.

[0095] When culturing solid culture medium, first cut different sized blocks of substrate according to the relative contents of the sugar substrate layer, inorganic salt substrate layer, and trace element substrate layer. Place each substrate block in a beaker and liquefy them by heating the beaker at a temperature between 98-100℃, stirring constantly to prevent settling and ensuring uniform mixing of all components. Stop heating when all the agar has been converted to liquid state, and place the beaker in a sterile environment to cool naturally. When the temperature drops to 65-75℃, add other components. Remove the solid culture medium from the beaker, cut it, and place it in a petri dish. Then add other solid components according to the cell culture conditions.

Claims

1. A sealed material handling device, characterized in that: include: Sealed containers for use in sealed material handling environments; Storage cylinders are disposed within the sealed container, and multiple storage cylinders are provided. A support plate is fixedly disposed in the sealed container, and multiple storage cylinders are also fixedly disposed on the support plate; A weighing platform is rotatably mounted on the bottom inside the sealed container; A material receiving cup, set on the supporting platform, is capable of receiving the raw materials in the storage cylinder and weighing the raw materials through the weighing platform; The storage tank includes several powder cylinders for storing powdered raw materials and water cylinders for storing distilled water. A support plate is fixedly installed on the top of the support plate, and a drive gear is rotatably installed on the top of the support plate. The drive gear drives the powder cylinders to feed the raw materials through the transmission component A under the drive component. A disc is fixedly installed at the bottom of the sealed tank, and a weighing platform is rotatably installed on the outside of the disc through a slip ring. The drive component drives the weighing platform to rotate intermittently through the transmission component B. The bottom of the sealed container is fixedly provided with a base, and the outer side of the sealed container is provided with a pick-up and put-out port for picking up the material cup. The inner side of the pick-up and put-out port is rotatably provided with a sealing door for sealing the sealed container. A display controller for controlling the operation of the device is fixedly provided on one side of the base. A sealing cover A is fixedly provided on the top of the sealed container. A bearing plate for assembling the drive assembly is fixedly provided on the inner side of the sealed container. A discharge pipe is fixedly installed at the bottom of the powder cylinder. A rotating shaft is rotatably installed on the inner side of the powder cylinder via a bearing bracket. A screw feeder is fixedly installed on the outer side of the rotating shaft inside the discharge pipe. A stirring rod is fixedly installed on the outer side of the rotating shaft inside the powder cylinder. A sealing cover B is fixedly installed at the top of the powder cylinder. A sealing cap is fixedly installed on the top of the sealing cover B. A worm gear is fixedly installed on the top of the rotating shaft outside the sealing cover B. The drive assembly drives the worm gear to rotate through the transmission assembly A. The drive assembly includes a motor, which is fixedly mounted on the top of the support plate. Both output ends of the motor are fixedly mounted with drive shafts. The top of the drive shaft is fixedly mounted with a drive gear, and the bottom end of the drive shaft drives the weighing platform to slide through the transmission assembly B. The support plate is fixedly mounted on the top of the support plate by a fixing plate. Support arms are evenly fixedly mounted on the outer side of the support plate. The transmission component A includes a driven gear, which is rotatably mounted on the top of the support arm. A bevel gear A is rotatably mounted on the bottom of the support arm and coaxially with the driven gear. A bevel gear B is meshed on the outer side of the bevel gear A. The bevel gear B is rotatably mounted on the outer side of the U-shaped seat via a connecting shaft. A worm gear is rotatably mounted on the inner side of the U-shaped seat via a connecting shaft. The worm gear meshes with the worm wheel in the powder cylinder. A connecting plate is fixedly mounted on the top of the U-shaped seat. The other end of the connecting plate is rotatably mounted on the support arm. An electric push rod A is rotatably mounted on the outer side of the connecting plate. The other end of the electric push rod A is rotatably mounted on the bottom of the support arm. The transmission component B includes a slide rail, which is fixedly mounted on the outside of the weighing platform. Toothed plates are symmetrically slidably mounted on the outside of the slide rail. An electric push rod B is fixedly mounted between the toothed plates. Engaging gears are meshed between the symmetrically mounted toothed plates. The engaging gears rotate under the drive of the drive component.

2. The sealing material handling device according to claim 1, characterized in that: A water valve is fixedly installed at the bottom of the water cylinder. A push rod for driving the water valve is slidably installed inside the water cylinder. An inclined pressure block A is fixedly installed at the top of the push rod. An inclined pressure block B is slidably installed on the inclined surface of the inclined pressure block A. A connecting arm is fixedly installed at the top of the inclined pressure block B. An electric push rod A is rotatably installed on the outside of the connecting arm. The electric push rod A is rotatably installed with one of the support arms on the outside of the support plate.

Images