Lotus embryo extract, preparation method and application in anti-aging and skin-repairing cosmetics

Abstract

The present invention discloses a lotus embryo extract, a preparation method thereof, and an application in anti-aging and skin-repairing cosmetics. The preparation method of the lotus embryo extract includes: selecting fresh water lily flowers, removing the petals, and taking out the receptacle; cutting the receptacle to extract the embryo content; mixing the obtained embryo content with water, and then stirring at 40-80 °C and 500-200 rpm for 1-3 hours to obtain a mixture; centrifuging the mixture at 2000-4000 rpm for 5-15 minutes, and collecting the supernatant after centrifugation; concentrating the supernatant to control its relative density at 1.02-1.12. By specifically extracting the effective components of the lotus embryo, the present invention effectively utilizes its pharmacological activity and successfully avoids the cross-confusion between the effective components. In addition, it also creates a brand-new anti-aging and skin-repairing cosmetic, which is expected to effectively resist aging and repair the skin, and fully exert the pharmacological effect of the lotus embryo.

Description

Technical Field

[0001] The present invention relates to a plant extract, and in particular to a lotus embryo extract, a preparation method thereof, and an application in anti-aging and anti-aging cosmetics. Background Art

[0002] Lotus is a vital aquatic plant with a long life cycle and can thrive in various environmental conditions. Its rhizome coils like a vine, plump and containing abundant air channels inside. There are black scale leaves growing above each section, and adventitious roots like whiskers growing below. The lotus leaf presents a shield-shaped outline with a diameter of up to 90 cm, slightly wavy at the edge, the leaf surface is smooth and covered with white powder, and the veins radiate outwards from the center, forming a radial pattern. The diameter of the lotus flower is between 10 and 20 cm, the petal shapes are diverse, the lotus embryo (i.e., the lotus ovary) has a diameter between 2 and 10 cm, the hard nut is oval or ovoid, and the lotus seeds are also these two shapes.

[0003] Lotus has occupied an important position in the history of culture, and its symbolic meaning of purity is thought-provoking. Its lotus root has a strong ability to purify water quality, can effectively absorb harmful heavy metal ions, and makes a great contribution to the ecological environment. Lotus can be used to make tea, its petiole and flower stalk are both edible, and the whole plant can be used for medicinal purposes and the creation of wetland natural landscapes. As a traditional medicine, lotus is also widely used to treat diseases such as indigestion and hemorrhoids. Modern pharmaceutical research has found that lotus petals are rich in various flavonoids, flavonoid glycosides, anthocyanins, and phenolic acids such as gallic acid, and have good antioxidant, anti-inflammatory, and anti-tumor and other biological activities.

[0004] However, past research and development often treated the lotus as a complete whole without considering the differences in the characteristics of its various parts. Although this method is simple, it actually cannot maximize the utilization of all the active ingredients of the lotus. Because each part has its unique chemical composition and pharmacological activity, if extracted without distinction, it may lead to the mixing of active ingredients, thereby affecting the concentration and effect of each active ingredient.

[0005] In the pharmaceutical or cosmetics industry, this problem is particularly serious. Because the active ingredients of the lotus are not accurately extracted, it may affect the effect of the product, reduce the potency of specific ingredients, and even cause adverse reactions. For example, if the active ingredients of the lotus are not accurately extracted, the desired antioxidant and anti-inflammatory effects may not be achieved.

[0006] Current research mainly focuses on aspects such as the collection of lotus germplasm resources, the cultivation of new varieties, the composition of lotus essential oil, the mechanism of flower color formation, the analysis of nutrients in petals and stems, and chemical composition analysis. However, there is currently little research on lotus embryos. Lotus embryos are important organs for plant reproduction, and their extracts contain unique chemical components. If the effective components of lotus embryos can be specifically extracted and utilized, it will undoubtedly greatly improve their application value in the fields of medicine and cosmetics. Summary of the Invention

[0007] The main idea of the inventor is to create an extraction technology specifically for the unique effective components of lotus embryos. The inventor believes that by specifically extracting the effective components of lotus embryos independently, not only can the unique active components be utilized to the maximum extent, increasing the concentration of these components in cosmetics, thereby improving the efficacy of cosmetics. More importantly, this method can avoid cross - confusion of active components and ensure the purity of lotus embryo components in cosmetics, which means that the properties and effects of cosmetics can be more precisely controlled.

[0008] Another core idea is to apply the beneficial components of lotus embryos to the production of anti - aging and repair cosmetics. Considering the pharmacological activity of lotus embryos, the inventor expects to develop a new type of anti - aging and repair cosmetic by specifically extracting and utilizing the effective components of lotus embryos.

[0009] Based on this, the inventor has completed the following invention content:

[0010] In the first aspect of the present invention, a method for preparing a lotus embryo extract is provided, including the following steps:

[0011] Cut the receptacle of the lotus flower and take out the embryo content;

[0012] Mix and stir the embryo content with water;

[0013] Centrifuge to obtain the supernatant;

[0014] Concentrate.

[0015] Preferably, a method for preparing a lotus embryo extract includes the following steps:

[0016] Cut the receptacle of the lotus flower and take out the embryo content;

[0017] Mix and stir the embryo content with water, and the mass ratio of the embryo content to water is (20 - 60):100;

[0018] Centrifuge to obtain the supernatant;

[0019] Concentrate the supernatant to a relative density of 1.02 - 1.12.

[0020] Preferably, a method for preparing a lotus embryo extract comprises the following steps:

[0021] Select fresh water lily flowers during the full-bloom period, remove the petal parts outside the flowers, and then take out the receptacle;

[0022] Cut the taken-out receptacle, and extract the embryo content by extrusion;

[0023] Mix the obtained embryo content with water, and then stir at 40 - 80 °C and 500 - 200 rpm for 1 - 3 hours to obtain a mixture; the mass ratio of the embryo content to water is (20 - 60):100;

[0024] Centrifuge the mixture at a rotation speed of 2000 - 4000 rpm for 5 - 15 minutes, and collect the supernatant after centrifugation;

[0025] Concentrate the supernatant, and control its relative density to be 1.02 - 1.12 to obtain the water lily lotus embryo extract.

[0026] Preferably, a method for preparing a lotus embryo extract comprises the following steps:

[0027] Cut the receptacle of the lotus flower, and take out the embryo content;

[0028] Mix and stir the embryo content with an enzyme-containing aqueous solution;

[0029] Centrifuge to obtain the supernatant;

[0030] Concentrate;

[0031] The enzyme-containing aqueous solution contains zinc chloride, an enzyme, and a nonionic surfactant.

[0032] Preferably, the enzyme is at least one of polyphenol oxidase and glycosidase.

[0033] Preferably, the enzyme-containing aqueous solution is composed of the following raw materials in mass percentages:

[0034] 0.04 - 0.1% zinc chloride;

[0035] 0.3 - 0.7% enzyme;

[0036] 0.1 - 0.5% nonionic surfactant;

[0037] The balance is water.

[0038] More preferably, a method for preparing a lotus embryo extract comprises the following steps:

[0039] Select fresh water lily flowers during the full-bloom period, remove the petal parts outside the flowers, and then take out the receptacle;

[0040] Cut the taken receptacle and extract the embryo content by extrusion;

[0041] Mix the obtained embryo content with an enzyme-containing aqueous solution, and then stir at 40 - 80 °C and 500 - 200 rpm for 1 - 3 hours to obtain a mixture; the mass ratio of the embryo content to water is (20 - 60):100;

[0042] Centrifuge the mixture at 2000 - 4000 rpm for 5 - 15 minutes, and collect the supernatant after centrifugation;

[0043] Concentrate the supernatant and control its relative density to be 1.02 - 1.12 to obtain the water lily embryo extract;

[0044] The enzyme-containing aqueous solution is composed of the following raw materials by mass percentage: 0.04 - 0.1% zinc chloride, 0.3 - 0.7% enzyme, 0.1 - 0.5% non-ionic surfactant, and the balance is water.

[0045] In the second aspect of the present invention, there is provided a water lily embryo extract prepared by the above method.

[0046] In the third aspect of the present invention, there is provided an application of the water lily embryo extract in the preparation of a repair and anti-aging cosmetic.

[0047] In the fourth aspect of the present invention, there is provided a repair and anti-aging cosmetic containing the above water lily embryo extract.

[0048] Preferably, the repair and anti-aging cosmetic contains 1 - 10 wt% of the water lily embryo extract.

[0049] Preferably, the cosmetic is a facial mask, foundation, primer, lotion, essence, moisturizing oil, nutrient cream, powder cake, facial cleanser, shampoo, emulsion or ointment.

[0050] The beneficial effects of the present invention are shown in the following aspects:

[0051] Special extraction: The present invention has developed a special extraction method for the active ingredients of water lily embryos. This strategy is beneficial to maximizing the utilization of the unique active ingredients contained in water lily embryos and can effectively prevent the interaction and interference between various active ingredients.

[0052] Anti-aging and repair efficacy: By using the active ingredients of water lily embryos in the production of cosmetics, the present invention creates a new type of anti-aging and repair cosmetic. Considering the pharmacological effects of water lily embryos themselves, it is expected that the cosmetics of the present invention will be able to effectively resist aging and repair the skin, thus exerting significant effects.

[0053] In summary, by specifically extracting the active ingredients of lotus embryos, the present invention effectively utilizes their pharmacological activities and successfully avoids cross - confusion between active ingredients. In addition, it has created a brand - new anti - aging and repair cosmetic, which is expected to effectively resist aging and repair the skin, giving full play to the pharmacological effects of lotus embryos. Embodiment

[0054] A method for preparing a lotus embryo extract, comprising the following steps:

[0055] Select fresh water lily flowers during the full - bloom period, remove the petal parts outside the flowers, and then take out the receptacle; this step is to obtain the best raw materials. During the full - bloom period, the pharmacological active ingredients of lotus embryos are the most abundant, so it is most ideal to collect at this time. The outer petal parts of the flowers are irrelevant to the present invention and should be removed. The embryo contents contained in the receptacle are important raw materials for the present invention.

[0056] Cut the taken - out receptacle, and extract the embryo contents (i.e., the lotus ovary) by extrusion; through cutting and extrusion, the embryo contents can be obtained to the maximum extent, which is the part rich in active ingredients.

[0057] Mix the obtained embryo contents with an enzyme - containing aqueous solution, and then stir at 40 - 80 °C and 500 - 200 rpm for 1 - 3 hours to obtain a mixture; the mass ratio of the embryo contents to water is (20 - 60):100; this step is to better dissolve the active ingredients in the embryo contents to form a uniform mixture. The selection of temperature and stirring speed, as well as the duration of stirring, are all to ensure the maximum dissolution of active ingredients. The enzyme in the enzyme - containing aqueous solution can help decompose the complex components in the lotus embryo, making the active ingredients more easily extracted.

[0058] Centrifuge the mixture at 2000 - 4000 rpm for 5 - 15 minutes, and collect the supernatant after centrifugation; centrifugation is for further purification, separating the solid impurities and active ingredients in the mixture, and only retaining the supernatant, which contains the active ingredients we need.

[0059] Concentrate the supernatant, control its relative density to be 1.02 - 1.12, to obtain the water lily lotus embryo extract; the purpose of concentration is to increase the concentration of active ingredients, so that the final extract contains a higher concentration of active ingredients. By controlling the relative density, the quality and activity of the extract can be ensured.

[0060] The enzyme-containing aqueous solution is composed of the following raw materials by mass percentage: 0.04 - 0.1% zinc chloride, 0.3 - 0.7% enzyme, 0.1 - 0.5% non-ionic surfactant, and the balance is water; this step describes the method for preparing the enzyme-containing aqueous solution. The ratios of zinc chloride, enzyme, and non-ionic surfactant all contribute to improving the dissolution and extraction of the active ingredients. Zinc chloride is used to provide zinc ions in this process to help improve the activity and stability of the enzyme used. However, it must be used with care because excessive metal ions will inhibit the enzyme activity. The main functions of the surfactant are: increasing the contact surface between the embryo content and the enzyme, enabling the enzyme to act more effectively on the embryo content; reducing the surface tension between the cell wall and the embryo content, making it easier for the enzyme to penetrate the cell wall, thereby improving the efficiency of the enzyme's action.

[0061] The enzyme is at least one of polyphenol oxidase and glycosidase; preferably, the enzyme is a mixed enzyme of polyphenol oxidase and glycosidase with a mass ratio of (1 - 2):(1 - 2). The selection of the enzyme is based on its role in the extraction process. Polyphenol oxidase can oxidize polyphenolic substances, while glycosidase can cleave glycosidic bonds. The two act together to make the active ingredients in the embryo content more easily extracted.

[0062] The non-ionic surfactant is alkylamide, alkyl glucoside, fatty alcohol polyoxyethylene ether, or fatty alcohol polyglycerol ester.

[0063] In each example:

[0064] The water lily is a tropical water lily provided by Zhuhai Green Future Biotechnology Co., Ltd.

[0065] AEO-9, a fatty alcohol polyoxyethylene ether from POLYCHEM, Indonesia.

[0066] Polyphenol oxidase, with an enzyme activity of 25,000 u / g, purchased from Huazhong Haiwei (Beijing) Gene Technology Co., Ltd.

[0067] The glycosidase used in the examples is β-glucosidase, with an enzyme activity of 2000 U / g, purchased from Jiangsu Aofu Biotechnology Co., Ltd. Example 1

[0068] A method for preparing a lotus embryo extract, comprising the following steps:

[0069] First, select fresh water lily flowers during the full bloom period, remove the petal parts outside the flowers, and then take out the receptacle;

[0070] Next, cut the taken-out receptacle and extract the embryo content by extrusion;

[0071] Mix the obtained 300 g of embryo content with 1000 g of water, and then stir at a speed of 100 rpm under the condition of 50 °C for 2 hours;

[0072] After stirring, centrifuge the mixture at a speed of 3000 rpm for 10 minutes. Then collect the supernatant after centrifugation;

[0073] Finally, concentrate the collected supernatant until its relative density reaches 1.06 to obtain the water lily embryo extract. Example 2

[0074] A method for preparing a lotus embryo extract, comprising the following steps:

[0075] First, select fresh water lily flowers during the full bloom period, remove the petal parts outside the flowers, and then take out the receptacle;

[0076] Next, cut the taken-out receptacle and extract the embryo content by extrusion;

[0077] Mix the obtained 300 g of embryo content with 1000 g of enzyme-containing aqueous solution, and then stir at a speed of 100 rpm under the condition of 50 °C for 2 hours;

[0078] After stirring, centrifuge the mixture at a speed of 3000 rpm for 10 minutes. Then collect the supernatant after centrifugation;

[0079] Finally, concentrate the collected supernatant until its relative density reaches 1.06 to obtain the water lily embryo extract.

[0080] The enzyme-containing aqueous solution is composed of the following raw materials by mass percentage: 0.07% zinc chloride, 0.5% enzyme, 0.3% non-ionic surfactant AEO-9, and the balance is water.

[0081] The enzyme is polyphenol oxidase. Example 3

[0082] The difference from Example 2 is that: the enzyme is glycosidase. Example 4

[0083] The difference from Example 2 is that: the enzyme is a mixed enzyme of polyphenol oxidase and glycosidase with a mass ratio of 2:1. Example 5

[0084] The difference from Example 2 is that: the enzyme is a mixed enzyme of polyphenol oxidase and glycosidase with a mass ratio of 1:1. Example 6

[0085] It is different from Example 2 in that the enzyme is a mixed enzyme of polyphenol oxidase and glycosidase with a mass ratio of 1:2. Example 7

[0086] It is different from Example 2 in that the enzyme-containing aqueous solution is composed of the following raw materials by mass percentage: 0.07% zinc sulfate, 0.5% polyphenol oxidase, 0.3% non-ionic surfactant AEO-9, and the balance is water. Example 8

[0087] It is different from Example 2 in that the enzyme-containing aqueous solution is composed of the following raw materials by mass percentage: 0.5% polyphenol oxidase, 0.3% non-ionic surfactant AEO-9, and the balance is water. Example 9

[0088] It is different from Example 2 in that the enzyme-containing aqueous solution is composed of the following raw materials by mass percentage: 0.07% zinc chloride, 0.5% polyphenol oxidase, and the balance is water. Example 10

[0089] It is different from Example 2 in that the enzyme-containing aqueous solution is composed of the following raw materials by mass percentage: 0.12% zinc chloride, 0.5% polyphenol oxidase, 0.3% non-ionic surfactant AEO-9, and the balance is water.

[0090] Test Example 1

[0091] I. Test Principle and Basis

[0092] The anatomy and histology of human skin indicate that the sebaceous gland is a vesicular gland, which mostly opens at the upper part of the hair follicle. Its secretion also increases after the development of secondary sexual characteristics and is significantly affected by male hormones. The sebaceous gland is an important gland attached to the skin and is an important site for producing sebum. It can secrete sebum, and the sebum enters the hair follicle and is discharged to the skin surface through the pores. The sebaceous gland is a target organ of androgens. Testosterone enlarges the volume of the sebaceous gland and increases its secretion. Especially when the 5α-reductase, a catalyst for sebum synthesis in the human body, is overly active, it causes an increase in the secretion of dihydrotestosterone (DHT, a male hormone), which prompts the sebaceous gland to malfunction and enlarge. 5α-reductase is the enzyme in the human body that converts free testosterone into DHT. Without 5α-reductase, DHT cannot be formed. Therefore, by inhibiting the activity of 5α-reductase to reduce the DHT level, it can effectively relieve the excessive secretion of sebum by the sebaceous gland, thereby achieving the effect of oil control.

[0093] II. Test Purpose

[0094] This experiment aims to detect the efficacy of the lotus leaf extract of this product as an oil-control cosmetic in slowing down sebum secretion and deposition at the application site, or making the oiliness at the application site less obvious; the oil-control efficacy of the substance is mainly evaluated by detecting the inhibition rate of the product on 5α-reductase.

[0095] III. Tested substances

[0096] Test sample: The test concentration is 80 mg / mL;

[0097] Positive control: The test concentration of finasteride solution is 2.5 mmol / L.

[0098] IV. Test procedures

[0099] 1) Weigh an appropriate amount of the sample for pretreatment;

[0100] 2) Add reagents to the pretreated sample solution step by step to make the reaction complete;

[0101] 3) At the same time, take an appropriate amount of the positive control and add reagents to make the reaction complete;

[0102] 4) Transfer an appropriate amount of the completely reacted reagent and measure its absorbance on the machine;

[0103] 5) Record the data and calculate the inhibition rate of 5α-reductase and the statistical difference value.

[0104] V. Result calculation

[0105] 5α-reductase is a membrane protease dependent on nicotinamide adenine dinucleotide phosphate (NADPH), which can irreversibly convert testosterone (T) into dihydrotestosterone (DHT). Calculate the ability of the test sample to inhibit 5α-reductase and calculate the statistical difference P value to determine whether the sample has oil-control efficacy.

[0106] Where: A 样品 : Represents the absorbance of the sample tube;

[0107] B 样品底物 : Represents the absorbance of the sample background tube;

[0108] C: Represents the absorbance of the solvent tube;

[0109] D: Represents the absorbance of the solvent background tube.

[0110] The results of this experiment were characterized by the inhibition rate of the sample against 5α-reductase. When compared with a control substance with oil-control efficacy, if the inhibition rate of the positive control was higher than that of the blank control group and the statistical difference value (P < 0.05), the test results were valid. If the inhibition rate of the test sample was higher than that of the blank control group and the statistical difference value (P < 0.05), it indicated that the test sample had oil-control efficacy. The experiment was conducted in three parallel tests, and the average value of the three parallel tests was used as the test result, as shown in Table 1.

[0111] The P value in each case was < 0.05.

[0112] Table 1: Table of Inhibition Rate Measurement Results

[0113] 5α - Reductase inhibition rate, % Blank control group 0.65 Positive control group 73.98 Example 1 31.28 Example 2 42.55 Example 3 40.11 Example 4 44.27 Example 5 46.30 Example 6 43.84 Example 7 40.92 Example 8 36.14 Example 9 40.47 Example 10 38.22

[0114] The main difference between Example 1 and Examples 2 - 9 lies in the enzymatic hydrolysis step. In Example 1, the enzymatic hydrolysis step was not carried out, while in Examples 2 - 9, different enzymes and enzyme ratios were added during the extraction process. We can see that the 5α-reductase inhibition rates of Examples 2 - 9 with the enzymatic hydrolysis step were generally higher than those of Example 1 without the enzymatic hydrolysis step. This shows that the enzymatic hydrolysis step plays an important role in increasing the 5α-reductase inhibition rate.

[0115] By comparing Examples 4 - 6 (using a complex enzyme of polyphenol oxidase and glycosidase) with Examples 2 - 3 (using a single enzyme), it can be seen that in terms of the ability to inhibit 5α - reductase, the effect of the complex enzyme is significantly better than that of the single enzyme. First, in Example 2, a single polyphenol oxidase was used, and the inhibition rate was 42.55%, while in Example 3, a single glycosidase was used, and the inhibition rate was 40.11%. When using the complex enzyme, such as in Example 4 (a mixed enzyme of polyphenol oxidase and glycosidase with a mass ratio of 2:1), Example 5 (a mixed enzyme of polyphenol oxidase and glycosidase with a mass ratio of 1:1), and Example 6 (a mixed enzyme of polyphenol oxidase and glycosidase with a mass ratio of 1:2), the inhibition rates were 44.27%, 46.30%, and 43.84% respectively, and these values are all better than the inhibition rates of using a single enzyme. The possible reason for this result is that the complex enzyme plays a synergistic role during the enzymatic hydrolysis process, improving the extraction efficiency, and thus enhancing the 5α - reductase inhibition ability of the final product. Specifically, polyphenol oxidase and glycosidase may act on different targets or pathways during the enzymatic hydrolysis process, enabling the more comprehensive release of active ingredients in the extract. For example, polyphenol oxidase may promote the oxidation of polyphenols, while glycosidase may help with the depolymerization of glycosides, and the synergistic effect of the two can achieve a more efficient extraction effect. Additionally, from the results of Examples 4 - 6, the ratio of polyphenol oxidase and glycosidase also has a certain impact on the final inhibition rate, and the mixed enzyme with a mass ratio of 1:1 shows the best performance in terms of the inhibition rate. This may indicate that an appropriate enzyme ratio can better exert the synergistic effect of the enzymes, thereby improving the extraction effect. Generally speaking, these results show that when extracting active substances from lotus embryos, using a complex enzyme and adjusting the appropriate enzyme ratio can significantly improve the extraction efficiency and the ability to inhibit 5α - reductase, further enhancing its anti - wrinkle and firming effects.

[0116] The main difference between Example 2 and Example 7 lies in the different zinc ion compounds used. In Example 2, zinc chloride was used, while in Example 7, zinc sulfate was used. The main role of the zinc ion compound in this extraction process is to act as a co - factor for the enzyme, helping to maintain the active structure of the enzyme, thereby enhancing its role during the enzymatic hydrolysis process. From the test results of Example 2 and Example 7, the 5α - reductase inhibition rate of Example 2 was 42.55%, while that of Example 7 was 40.92%. This shows that using zinc chloride as the co - factor for the enzyme is better than using zinc sulfate, with a relatively large difference.

[0117] The main difference between Example 2 and Example 8 is that no zinc ion compound, namely zinc chloride, is added to the aqueous enzyme solution. As mentioned above, zinc chloride mainly acts as a cofactor for the enzyme in this process, helping to maintain the active structure of the enzyme and further enhancing the action of the enzyme. Therefore, removing zinc chloride will affect the enzyme activity and extraction effect. From the test data, the 5α-reductase inhibition rate of Example 2 is 42.55%, while that of Example 8 is 36.14%. This shows that the inhibition rate of Example 8 without zinc chloride is lower.

[0118] The main difference between Example 2 and Example 9 is that no non-ionic surfactant is added to the aqueous enzyme solution. This surfactant is generally used to enhance the contact surface between the enzyme and substances in the solution and improve the enzymatic hydrolysis efficiency. As we can see from the table, the 5α-reductase inhibition rate of Example 2 is 42.55%, while that of Example 9 is 40.47%. This indicates that the inhibition rate of Example 9 without non-ionic surfactant is slightly lower than that of Example 2. The main function of the non-ionic surfactant is to reduce the surface tension between water and other substances and increase the contact area between the enzyme and the substrate to be enzymatically hydrolyzed, thereby improving the enzymatic hydrolysis efficiency. Therefore, in Example 9, due to the absence of non-ionic surfactant, the enzymatic hydrolysis efficiency may be relatively reduced, and thus its 5α-reductase inhibition rate is slightly lower than that of Example 2. This result once again confirms the important role of non-ionic surfactant in the enzymatic hydrolysis process, which can improve the enzymatic hydrolysis efficiency and thus the extraction effect.

[0119] The main difference between Example 2 and Example 10 is the addition amount of zinc chloride. The mass percentage of zinc chloride in Example 2 is 0.07%, while that in Example 10 is 0.12%. From the inhibition rate measurement results table, we can see that the 5α-reductase inhibition rate of Example 2 is 42.55%, while that of Example 10 is 38.22%. The inhibition rate of Example 10 is significantly lower than that of Example 2. Zinc chloride mainly acts as a cofactor for the enzyme in this process to help maintain the enzyme activity, but adding too much zinc chloride may inhibit the enzyme activity and cause its activity to decrease. This may be the main reason why the inhibition rate of Example 10 is lower than that of Example 2. This result shows that during the use of enzyme cofactors, an appropriate addition amount needs to be controlled. Excessive addition may inhibit the enzyme activity and instead reduce the enzymatic hydrolysis efficiency.

[0120] The above embodiments are only the preferred embodiments of the present invention and cannot be used to limit the scope of protection of the present invention. Any non-substantial changes and substitutions made by those skilled in the art based on the present invention fall within the scope of protection required by the present invention.

Claims

1. A method for preparing a lotus embryo extract, comprising the following steps: Cut the receptacle of the lotus flower and take out the embryo content; Mix and stir the embryo content with an enzyme-containing aqueous solution; Centrifuge to obtain the supernatant; Concentrate; The enzyme-containing aqueous solution contains zinc chloride, an enzyme and a nonionic surfactant; The enzyme is a mixed enzyme of polyphenol oxidase and β-glucosidase with a mass ratio of (1-2):(1-2); The enzyme-containing aqueous solution is composed of the following raw materials in mass percentage: 0.04-0.1% zinc chloride; 0.3-0.7% enzyme; 0.1-0.5% nonionic surfactant; The balance is water; The nonionic surfactant is fatty alcohol polyoxyethylene ether.

2. A lotus embryo extract, characterized in that, Prepared by the method according to claim 1.

3. Use of the lotus embryo extract according to claim 2 in the preparation of a skin oil-control cosmetic.

4. An oil-control cosmetic, characterized in that, Contains the lotus embryo extract according to claim 2.

5. The oil-control efficacy cosmetic according to claim 4, characterized in that, The cosmetic is a facial mask, foundation, primer, lotion, essence, moisturizing oil, nourishing cream, powder cake, facial cleanser, shampoo, emulsion or ointment.

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