Application of Trichostatin in reducing the activities of plant lignin peroxidase and polygalacturonase and / or increasing the activities of cellulase and hemicellulase

Abstract

The present invention provides the application of trichostatin in reducing the activities of plant lignin peroxidase and polygalacturonase, and / or increasing the activities of cellulase and hemicellulase. Taking pepper as an example, spraying the preparation of the present invention can improve the lodging resistance of pepper and enhance the resistance of pepper seedlings, and has good application prospects in the field of pepper planting technology. In addition, the preparation method of the preparation of the present invention is simple and the application is convenient.

Description

[0001] This application is a divisional application of the application with the application number 2022102294292, the application date of January 31, 2021, and the invention title of: Application of Trichostatin in Preventing Plant Lodging and Lodging Prevention Reagent. Technical Field

[0002] The present invention relates to crop regulators, and specifically relates to the application of trichostatin in reducing the activities of plant lignin peroxidase and polygalacturonase and / or increasing the activities of cellulase and hemicellulase. Background Art

[0003] Capsicum annuum L. is an annual or perennial herbaceous plant of the genus Capsicum in the Solanaceae family and is the largest vegetable crop in China. During the growth period of Capsicum annuum L., due to the influence of internal and external factors, lodging of the plants will occur. The manifestations are: the stems of the plants are bent. The lodging plants cannot grow upright normally, so that the whole plant remains in a prostrate state during the growth period, resulting in weakened resistance of Capsicum annuum L., easy occurrence of pests and diseases, and lodging seriously affects the yield and quality of Capsicum annuum L.

[0004] In the production of Capsicum annuum L., lodging resistance is not only related to high yield, stable yield, high quality and production cost of Capsicum annuum L., but also related to mechanical harvesting, utilization of stem residues and environmental protection. Lodging resistance is an important agronomic trait of Capsicum annuum L. In production, changing cultivation measures and physical and chemical conditions are often used to control lodging resistance, such as fixing with a support frame, fixing with a hanging seedling, artificial soil cultivation, etc. These methods are easy to increase the labor intensity and production cost. Taking effective measures to prevent Capsicum annuum L. from lodging can enhance the resistance of Capsicum annuum L. plants and effectively reduce the occurrence of pests and diseases. Summary of the Invention

[0005] The trichostatin used in the present invention has the molecular formula C 17 H 22 N2O3 (abbreviation TSA). Applying trichostatin can significantly improve the lodging resistance of plants at the seedling stage and enhance the resistance of plants.

[0006] The plants include Capsicum annuum L.

[0007] The application specifically is to prevent plant lodging, especially to prevent plant lodging at the seedling stage.

[0008] Further, the trichostatin is applied to the plants or the culture medium, substrate or soil for cultivating plants.

[0009] Furthermore, the trichostatin is formulated into a solution for application.

[0010] The solvents include: water and / or DMSO, alcohols or ethers.

[0011] For the above application, the concentration range applied is 12 μmol / L - 288 μmol / L; preferably 48 μmol / L - 96 μmol / L.

[0012] The main object of the present invention is to provide the application of trichostatin in reducing the activities of plant lignin peroxidase and polygalacturonase, and increasing the activities of cellulase and hemicellulase.

[0013] The reagent of trichostatin used in the present invention has a solute including trichostatin, and the solvent is water and / or DMSO, alcohol or ether substances.

[0014] The plants described above include peppers.

[0015] Furthermore, the concentration range is 12 μmol / L - 288 μmol / L; preferably 48 μmol / L - 96 μmol / L.

[0016] When preparing the above solvent of the present invention, it is preferred to dissolve 5 mg of TSA in 5.513 mL of DMSO to prepare a 3 mmol / L mother liquor; dilute it with deionized water to 48 μmol / L and 96 μmol / L working solutions.

[0017] Preparation method of the 48 μmol / L working solution: Pipette 16 μL of the 3 mmol / L mother liquor into 1 L of deionized water, mix well, and store at -20 °C for standby.

[0018] Preparation method of the 96 μmol / L working solution: Pipette 32 μL of the 3 mmol / L mother liquor into 1 L of deionized water, mix well, and store at -20 °C for standby.

[0019] Furthermore, it is preferred to evenly spray the above-prepared working solution on the pepper leaves and the stem parts of the peppers.

[0020] Even further, spray the above reagent on peppers with a seedling age of 6 - 7 days, cultivate for 8 hours under dark conditions after spraying, then cultivate under light for 16 hours, and continuously and evenly spray the working solution for 20 days.

[0021] Advantages of the present invention:

[0022] The present invention provides the application of trichostatin in reducing the activities of plant lignin peroxidase and polygalacturonase, and / or increasing the activities of cellulase and hemicellulase. Taking peppers as an example, spraying the preparation of the present invention can improve the lodging resistance of peppers, enhance the resistance of pepper seedlings, and has good application prospects in the field of pepper planting technology. In addition, the preparation method of the preparation of the present invention is simple and the application is convenient. Description of the Drawings

[0023] Figure 1Pictures of the 20-day treatment with DMSO aqueous solutions for Comparative Example 1 and Comparative Example 2;

[0024] Figure 2 The changes in the upright and prostrate plants after 20 days of treatment in Example 2;

[0025] Figure 3 Cross-sectional pictures of the stem segments after 20 days of treatment in Comparative Example 1, Comparative Example 2, Example 1, and Example 2.

[0026] From Figure 1 and Figure 2 It can be seen that there is no obvious change in the prostrate type (PF) of Comparative Example 1, while the pepper seedlings sprayed with 96 μmol / L TSA of the present invention in the prostrate type (PF) of Example 2 have an obvious anti-lodging effect.

[0027] From Figure 3 It can be seen that the xylem area in the vascular bundle of the prostrate type (PF) in Example 1 is slightly increased compared with that of Comparative Example 1; compared with Comparative Example 1 and Example 1, the number of vascular bundles and the xylem area in Example 2 are both significantly increased, and the pith cavity volume is significantly reduced. It shows that spraying 96 μmol / L TSA of the present invention can significantly increase the number of vascular bundles and the xylem area to enhance the mechanical support of pepper seedlings, thereby improving the anti-lodging ability. Detailed implementation mode

[0028] The following examples are intended to further illustrate the present invention rather than limit the present invention.

[0029] It can be seen from Table 1 that the upright type of Comparative Example 2 did not lodge, and 92.86% of the prostrate type of Comparative Example 1 lodged; in Example 1, the upright type did not lodge, and 64.29% of the prostrate plants lodged; in Example 2, the upright type did not lodge, and only 15% of the prostrate plants lodged. It shows that spraying TSA in Example 1 and Example 2 can effectively reduce the lodging of prostrate plants, and the effect of Example 2 is better.

[0030] Table 1 Statistical table of lodging rates after 20 days of implementation in examples and comparative examples

[0031]

[0032] Example 1

[0033] Dissolve 5 mg of TSA in 5.513 mL of DMSO to prepare a 3 mmol / L stock solution; dilute it with deionized water to a 48 μmol / L working solution.

[0034] Preparation of 48 μmol / L working solution: Pipette 16 μL of the 3 mmol / L stock solution into 1 L of deionized water, mix well, and store at -20 °C for later use.

[0035] Using the 48 μmol / L TSA working solution prepared above, spray the 7-day-old prostrate and erect pepper seedlings. Continuously and evenly spray the TSA solution with a small sprayer in the dark, culture in the dark for 8 hours and in the light for 16 hours, continuously spray for 20 days, and the spraying amount is until liquid drips from both the pepper leaves and stems.

[0036] Example 2

[0037] Dissolve 5 mg of TSA in 5.513 mL of DMSO to prepare a 3 mmol / L stock solution; dilute it with deionized water to a 96 μmol / L working solution.

[0038] Preparation of 96 μmol / L working solution: Pipette 32 μL of the 3 mmol / L stock solution into 1 L of deionized water, mix well, and store at -20 °C for later use.

[0039] Using the 96 μmol / L TSA working solution prepared above, spray the 7-day-old prostrate and erect pepper seedlings. Continuously and evenly spray the TSA solution with a small sprayer in the dark, culture in the dark for 8 hours and in the light for 16 hours, continuously spray for 20 days, and the spraying amount is until liquid drips from both the pepper leaves and stems.

[0040] Comparative Example 1

[0041] Negative control. Compared with Example 1 and Example 2, it lacks TSA (the amounts of DMSO and deionized water are the same as in Example 1). The spraying object is 7-day-old prostrate pepper seedlings.

[0042] The spraying method is to continuously and evenly spray the same concentration of DMSO solution on 7-day-old prostrate pepper seedlings with a small sprayer in the dark for 20 days, and the spraying amount is until liquid drips from both the pepper leaves and stems.

[0043] Comparative Example 2

[0044] Positive control. Compared with Example 1 and Example 2, it lacks TSA (the amounts of DMSO and deionized water are the same as in Example 1). The spraying object is 7-day-old erect pepper seedlings.

[0045] The spraying method is to continuously and evenly spray the same concentration of DMSO solution on 7-day-old erect pepper seedlings with a small sprayer in the dark for 20 days, and the spraying amount is until liquid drips from both the pepper leaves and stems.

[0046] As can be seen from the results in Table 2, compared with Comparative Example 1, the total soluble protein and sucrose synthase (SS-I) in the stems of the prostrate plants in Example 2 decreased slightly; the sucrose synthase activity (SS-II) increased slightly, but there was no significant effect; similarly, compared with Comparative Example 2, the total soluble protein, sucrose synthase (SS-I) and sucrose synthase activity (SS-II) in the stems of the prostrate plants in Example 2 increased slightly, but there was no significant effect. This shows that after spraying TSA in Example 2, the normal physiological activities of the prostrate pepper seedlings can also be maintained.

[0047] Table 2 Comparison of soluble protein content and sucrose synthase activity

[0048]

[0049] As can be seen from Table 3, the activities of lignin peroxidase and polygalacturonase in the stems of the prostrate pepper plants in Example 2 were significantly lower than those in the prostrate pepper stems treated in Comparative Example 1, decreasing by 21.33% and 20% respectively. Compared with the upright plants in the positive control Comparative Example 2, the activity of lignin peroxidase in the prostrate plants decreased slightly, while the activity of polygalacturonase remained basically unchanged. It shows that the activities of lignin peroxidase and polygalacturonase in the prostrate plants are higher than those in the upright plants, resulting in lignin degradation and thus inducing plant lodging; however, Example 2 can reduce the activities of lignin peroxidase and polygalacturonase in the prostrate plants, reduce lignin degradation, and thus keep the prostrate plants in an upright phenotype.

[0050] Table 3 Enzyme activities related to lignin degradation

[0051]

[0052] As can be seen from Table 4, compared with Comparative Example 1, the decrease in the cellulase activity of the prostrate seedlings in Example 2 was only 0.98%, and this difference was not significant; compared with Comparative Example 2, the cellulase activity in Example 2 was still 10.90% higher. The xylanase activity in Example 2 increased by 2.14 times compared with Comparative Example 1, and compared with Comparative Example 1, the cellulase activity in Comparative Example 2 increased by 11%. This may be because under the stable "source-sink" relationship, the energy and elements required for lignin synthesis may come from the degradation of cellulose and hemicellulose. This shows that Example 2 can promote the increase in the activities of cellulase and xylanase, thereby providing more energy and elements for lignin synthesis and promoting the uprightness of the stems of the prostrate seedlings.

[0053] Table 4 Enzyme activities related to cellulose and hemicellulose degradation

[0054]

Claims

1. Application of trichostatin in reducing the activity of lignin peroxidase or polygalacturonase in pepper or increasing the activity of hemicellulase in pepper.

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