A dual-color visualization analysis and detection method for safflower toxins
By combining the rhodamine 6G-TMB system with the MNP@Ag-Apt-cDNA-Au/Pt signal probe, the problem of low sensitivity of TMB colorimetric development catalyzed by nanozymes was solved, and high-sensitivity detection of low concentrations of safflower toxin was achieved.
CN116908169BActive Publication Date: 2026-03-10HAINAN UNIV +1
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-06-20
- Publication Date
- 2026-03-10
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Figure CN116908169B_ABST
Abstract
This invention relates to the field of salicornin detection technology, and particularly to a dual-color visualization analysis and detection method for salicornin. The method includes: taking a shellfish sample to be tested, adding hydrochloric acid solution, boiling and stirring, and centrifuging at low temperature; collecting the supernatant and adjusting the pH of the supernatant to <4 with hydrochloric acid solution; adding PBS solution and mixing well; adding the test solution to a suspension of MNP@Ag-Apt-cDNA-Au / Pt signal probe and incubating at room temperature; magnetic separation, taking the supernatant and adding it to 4-7 times its volume of dual-color development solution, and incubating for 10-20 min; the dual-color development solution includes 1 mM rhodamine 6G, 0.6 mM TMB, and 1 mM hydrogen peroxide in a volume ratio of 0.3-1:10:10; taking images with a camera, extracting the RGB histogram data, analyzing the data, and determining the concentration of salicornin. The advantages are low detection limit and high sensitivity.
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Citation Information
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