A culture medium filtration device

Abstract

This invention discloses a culture medium filtration device, relating to the field of culture medium filtration technology, and solves the technical problem of culture medium being easily contaminated due to contact with the external environment during filtration. The device includes a filter box, a shell, and a container for preparing the culture medium. The shell is fitted onto the top of the filter box, and the container is inserted into the top of the shell and extends downwards. The container consists of a cap, a cylindrical portion, and a frustum-shaped portion from top to bottom. A first conical hole is provided at the top of the shell for the frustum-shaped portion to pass through. Two symmetrical inclined plates are rotatably mounted inside the shell, and a counterweight assembly is installed between the inclined plates and the shell. A partition is installed inside the frustum-shaped portion, and a second and third conical hole are respectively provided at the bottom of the partition and the frustum-shaped portion. This invention facilitates the isolation of the culture medium from the external environment during filtration, preventing contamination and ensuring the quality of the culture medium.

Description

Technical Field

[0001] This invention relates to the field of culture medium filtration technology, and more specifically to a culture medium filtration device. Background Technology

[0002] Culture medium refers to a specific substance used in the laboratory to cultivate and reproduce microorganisms. It is usually composed of carbon sources, nitrogen sources, inorganic salts, growth factors and water. Different types of microorganisms require different culture media to provide the necessary nutrients and environmental conditions.

[0003] Culture media may contain impurities such as solid particles, microbial residues, and bacteria. These impurities can interfere with cell growth and affect the purity of the culture. Filtration removes these impurities, ensuring the purity of the culture medium. Filtered media has higher transparency, allowing for better observation and assessment of microbial growth and improving culture results. Furthermore, filtration makes the composition of the culture medium more homogeneous, which is beneficial for microbial nutrient absorption.

[0004] A Chinese patent with authorization number CN217988497U discloses a liver cell culture medium filtration device. The device involves placing the culture medium to be filtered inside a filter cylinder. An electric telescopic rod retracts, causing the mounting box to move downwards and allowing a pressure block to enter the filter cylinder. The drive motor is activated; the left drive motor drives a corresponding incomplete gear to rotate clockwise, while the right drive motor drives a corresponding incomplete gear to rotate counterclockwise. When the incomplete gear meshes with the rack, it causes the rack and stop to move downwards, compressing a first spring. Simultaneously, the stop, via a sliding rod, pushes the pressure block downwards, squeezing the culture medium inside the filter cylinder. When the incomplete gear disengages from the rack, the compressed first spring extends, pushing the rack and stop upwards. Simultaneously, the stop, via the sliding rod, pushes the pressure block back to its original position. Thus, when the drive motor operates, it causes the pressure block to move up and down repeatedly, repeatedly squeezing the culture medium inside the filter cylinder, saving manpower and accelerating the filtration efficiency. However, this technical solution still has the following drawbacks:

[0005] While the up-and-down reciprocating movement of the pressure block can speed up the filtration process, placing the culture medium directly inside the filter cartridge during filtration causes the culture medium to come into contact with the external environment, posing a risk of contamination. Summary of the Invention

[0006] The purpose of this invention is to provide a culture medium filtration device that solves the problem that culture medium is easily contaminated due to contact with the external environment during the filtration process.

[0007] The objective of this invention can be achieved through the following technical solutions:

[0008] A culture medium filtration device includes a filter box, a shell, and a container for preparing culture medium. The shell is fitted onto the top of the filter box, and the container is inserted into the top of the shell and extends downward. The container is composed of a cap, a cylindrical part, and a frustum-shaped part from top to bottom. The top of the shell has a first conical hole through which the frustum-shaped part passes. Two symmetrical inclined plates are rotatably installed inside the shell. A counterweight assembly is installed between the inclined plates and the shell. A partition is installed inside the frustum-shaped part. The bottom of the partition and the frustum-shaped part have a second conical hole and a third conical hole, respectively. A matching first block and a second block are respectively provided above the second and third conical holes. A fixing rod connects the first and second block, and an elastic assembly is installed between the second block and the partition. A T-shaped rod for lifting the second block is installed inside the shell. A stepped groove is provided along the edge of the top of the filter box, and a matching filter frame is provided in the stepped groove. A matching filter screen is installed in the filter frame.

[0009] As a further aspect of the present invention: the counterweight assembly includes fixed pulleys symmetrically installed on the top of the housing, guide wheels are installed on the top of the housing near both sides, a suspension rope is connected to the inclined plate, a rope hole is opened on the top of the housing for the suspension rope to pass through, and the end of the suspension rope away from the inclined plate passes around the fixed pulley and the guide wheel in sequence and is connected to a counterweight block, and the counterweight block is slidably connected to the outer side wall of the housing.

[0010] As a further aspect of the present invention: the elastic component includes a cylinder symmetrically installed at the bottom of the partition, a piston slidably installed inside the cylinder, a slide rod connected to the bottom of the piston and connected to the second block, and a spring installed on the upper surface inside the cylinder, the bottom end of the spring being connected to the top of the piston.

[0011] As a further aspect of the present invention: a rectangular hole corresponding to the filter box is provided at the bottom of the housing, and guide blocks are installed at the four corners of the filter box near the top. Guide holes are provided through the guide blocks, and bolts passing through the guide holes are installed at the bottom of the housing, with matching locking nuts threaded onto the bolts.

[0012] As a further aspect of the present invention: a matching sealing ring is installed between the suspension rope and the rope hole.

[0013] As a further aspect of the present invention: a pressure frame is provided above the filter frame, an isolation frame is fixedly connected to the top of the pressure frame, and a sealing pressure pad that fits against the edge of the top of the filter frame is connected to the bottom of the pressure frame. The isolation frame is fixedly installed on the inner wall of the housing near the bottom end, and the two ends of the bottom of the T-shaped rod are fixedly connected to the two opposing inner walls of the isolation frame.

[0014] As a further aspect of the present invention: the inner diameter of the top end of the first conical hole, the second conical hole and the third conical hole are all larger than the inner diameter of the bottom end, and rubber pads are respectively installed in the first conical hole, the second conical hole and the third conical hole, and a discharge pipe is connected to the side wall of the filter box near the bottom.

[0015] As a further aspect of the present invention: the outer wall near the top of the cylindrical part is provided with external threads, the cover is threadedly connected to the cylindrical part, and two symmetrical inverted L-shaped rods are rotatably installed on the top of the housing. The horizontal part of the inverted L-shaped rods is provided with threaded rods, and the bottom of the threaded rods is provided with pressure blocks for applying pressure to the cover.

[0016] The beneficial effects of this invention are:

[0017] 1. In this invention, the culture medium is directly prepared by using a container. The container is inserted into the first conical hole at the top of the shell, which facilitates the formation of a closed state inside the shell. During the insertion of the container, the two inclined plates are pressed and automatically deflected to open. The second block is lifted by the T-shaped rod, which facilitates the synchronous rise of the second block and the first block, so that the culture medium in the container flows out automatically and is filtered through the filter screen. The entire filtration process is carried out in a closed space, which makes it less prone to contamination.

[0018] 2. In this invention, the counterweight component facilitates the automatic deflection and closure of the two inclined plates after the container is separated from the shell, thus preventing the external environment from contaminating the shell and the inside of the filter box after the container is separated.

[0019] 3. In this invention, bolts and locking nuts facilitate the connection between the shell and the filter box. The pressure frame facilitates the application of pressure to the filter box, ensuring the stability of the filter box installation position. The isolation frame and sealing gasket ensure that the culture medium will not flow out through the connection between the shell and the filter box when it falls through the container, nor will it enter the stepped groove, so that the culture medium can fall after being filtered by the filter screen. Attached Figure Description

[0020] The invention will now be further described with reference to the accompanying drawings.

[0021] Figure 1 This is a schematic diagram of the structure of the present invention;

[0022] Figure 2 This is a schematic diagram of the structure of the connection between the shell and the filter box in this invention;

[0023] Figure 3 This is a perspective view of the container in this invention;

[0024] Figure 4 This is a perspective view of the connection between the pressure frame and the isolation frame in this invention;

[0025] Figure 5 yes Figure 1 Enlarged view of section A;

[0026] Figure 6 yes Figure 2 Enlarged view of section B.

[0027] In the diagram: 1. Filter box; 2. Shell; 3. Container; 301. Cover; 302. Cylindrical part; 303. Frustum; 4. Inclined plate; 5. Partition; 6. First blocking block; 7. Second blocking block; 8. T-shaped rod; 9. Filter frame; 10. Filter screen; 11. Fixed pulley; 12. Guide wheel; 13. Lifting rope; 14. Counterweight; 15. Cylinder; 16. Piston; 17. Sliding rod; 18. Spring; 19. Guide block; 20. Locking nut; 21. Pressure frame; 22. Isolation frame; 23. Sealing gasket; 24. Discharge pipe; 25. Inverted L-shaped rod; 26. Threaded rod. Detailed Implementation

[0028] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0029] Please see Figure 1-3 As shown, the present invention is a culture medium filtration device, including a filter box 1, a shell 2, and a container 3 for preparing culture medium. The shell 2 is fitted onto the top of the filter box 1. A discharge pipe 24 is connected to the side wall of the filter box 1 near the bottom to facilitate the discharge of the filtered culture medium. The container 3 is inserted into the top of the shell 2 and extends downward. The container 3 is composed of a cap 301, a cylindrical part 302, and a frustum part 303 from top to bottom. The top of the shell 2 has a first conical hole through which the frustum part 303 passes. The outer side wall of the cylindrical part 302 near the top has an external thread. The cap 301 is threadedly fitted onto the cylindrical part 302. Two symmetrical inverted L-shaped rods 25 are rotatably installed on the top of the shell 2. A threaded rod 26 is provided through the horizontal part of the inverted L-shaped rods 25. A pressure block for applying pressure to the cap 301 is installed at the bottom of the threaded rod 26 to improve the stability of the container 3 installation.

[0030] See Figure 1 and Figure 5As shown, a partition 5 is installed inside the frustum portion 303. The bottom of the partition 5 and the frustum portion 303 are respectively provided with a second conical hole and a third conical hole. A matching first block 6 and a second block 7 are respectively provided above the second conical hole and the third conical hole. The inner diameter of the top end of the first conical hole, the second conical hole and the third conical hole are all larger than the inner diameter of the bottom end. Rubber gaskets are installed in the first conical hole, the second conical hole and the third conical hole to ensure the sealing when the frustum portion 303, the first block 6 and the second block 7 are connected to the first conical hole, the second conical hole and the third conical hole respectively. A fixing rod is connected between the first block 6 and the second block 7, and an elastic component is installed between the second block 7 and the partition 5.

[0031] The elastic component includes a cylinder 15 symmetrically installed at the bottom of the partition 5. A piston 16 is slidably installed inside the cylinder 15. The bottom of the piston 16 is connected to a slide rod 17 connected to the second block 7. A spring 18 is installed on the upper surface inside the cylinder 15. The bottom end of the spring 18 is connected to the top of the piston 16. The tension of the spring 18 is used to press the first block 6 and the second block 7 into the second conical hole and the third conical hole respectively, ensuring that the container 3 does not leak. A T-shaped rod 8 is installed inside the shell 2 to lift the second block 7, so that the culture medium in the container 3 can flow out.

[0032] See Figure 1-2 As shown, two symmetrical inclined plates 4 are rotatably installed inside the housing 2. A counterweight assembly is installed between the inclined plates 4 and the housing 2. The counterweight assembly includes fixed pulleys 11 symmetrically installed on the top of the housing 2, and guide wheels 12 installed on the top of the housing 2 near both sides. A suspension rope 13 is connected to the inclined plates 4. A rope hole is opened on the top of the housing 2 for the suspension rope 13 to pass through. A matching sealing ring is installed between the suspension rope 13 and the rope hole to ensure sealing. The end of the suspension rope 13 away from the inclined plates 4 passes around the fixed pulley 11 and the guide wheel 12 in sequence and is connected to a counterweight block 14. The counterweight block 14 generates gravity, which causes the suspension rope 13 to exert an upward pulling force on the inclined plates, so that the ends of the two inclined plates 4 fit together to isolate a closed space inside the housing 2. The counterweight block 14 is slidably connected to the outer wall of the housing 2.

[0033] See Figure 2 , Figure 4 and Figure 6 As shown, a stepped groove is provided at the top edge of the filter box 1, and a matching filter frame 9 is provided in the stepped groove. A matching filter screen 10 is installed in the filter frame 9 to facilitate the filtration of the culture medium. A rectangular hole corresponding to the filter box 1 is provided at the bottom of the shell 2. Guide blocks 19 are installed at the four corners near the top of the filter box 1. Guide holes are provided through the guide blocks 19. Bolts passing through the guide holes are installed at the bottom of the shell 2, and matching locking nuts 20 are threaded on the bolts to facilitate the disassembly and assembly between the shell 2 and the filter box 1.

[0034] A pressure frame 21 is provided above the filter frame 9. An isolation frame 22 is fixedly connected to the top of the pressure frame 21, and a sealing pressure pad 23 that fits against the edge of the top of the filter frame 9 is connected to the bottom of the pressure frame 21. This not only facilitates the pressing and positioning of the filter frame 9, but also prevents the culture medium from entering the stepped groove. The isolation frame 22 is fixedly installed on the inner wall of the shell 2 near the bottom. The two ends of the bottom of the T-shaped rod 8 are fixedly connected to the two sides of the inner wall facing each other of the isolation frame 22.

[0035] The working principle of this invention is as follows: A culture medium is prepared in container 3. When the culture medium needs to be filtered, the frustum 303 of container 3 is inserted into the first conical hole at the top of shell 2. The inverted L-shaped rod 25 is rotated to position the pressure block above the cap 301, and the threaded rod 26 is rotated to apply downward pressure to container 3. During the insertion of the frustum 303 into shell 2, pressure is applied to the two inclined plates 4, causing them to deflect and separate. This, combined with the fixed pulley 11 and guide wheel 12, causes the suspension rope 13 to pull the counterweight 14 upwards. When the bottom of the second block 7 contacts the top of the T-shaped rod 8, as container 3 descends, the T-shaped rod 8 lifts the second block 7, causing the first block 6 to rise synchronously. This causes the sliding rod 17 to drive the piston 1. 6. Slide up and compress spring 18. At this time, the culture medium in container 3 will flow out through the second and third conical holes. The flowing culture medium falls into the filter box 1 and is filtered by filter screen 10. The entire filtration process is isolated from the external environment, thus avoiding contamination of the culture medium. After filtration, container 3 is separated from shell 2. At this time, counterweight 14 descends and resets under the action of gravity, causing the two inclined plates 4 to deflect and close, so that the external environment will not affect the inside of shell 2, which is convenient for subsequent culture medium to continue to be filtered. When filter screen 10 needs to be cleaned, loosen locking nut 20 to separate shell 2 from filter box 1, so that filter frame 9 can be taken out from the stepped groove and the filter screen 10 can be cleaned.

[0036] The foregoing has provided a detailed description of one embodiment of the present invention, but this description is merely a preferred embodiment and should not be construed as limiting the scope of the invention. All equivalent variations and modifications made within the scope of the claims of this invention should still fall within the patent coverage of this invention.

Claims

1. A culture medium filtration device, comprising a filter box (1), a housing (2), and a container (3) for preparing a culture medium, wherein the housing (2) is fitted over the top of the filter box (1), and the container (3) is inserted into the top of the housing (2) and extends downward, characterized in that, The container (3) is composed of a cap (301), a cylindrical part (302), and a frustum (303) from top to bottom. The top of the shell (2) has a first conical hole through which the frustum (303) passes. Two symmetrical inclined plates (4) are rotatably mounted inside the shell (2). A counterweight assembly is installed between the inclined plates (4) and the shell (2). A partition (5) is installed inside the frustum (303). A second conical hole and a third conical hole are respectively opened at the bottom of the partition (5) and the frustum (303). A first block (6) and a second block (7) are respectively provided above the second conical hole and the third conical hole. A fixed rod is connected between the first block (6) and the second block (7). An elastic component is installed between the second block (7) and the partition (5). A T-shaped rod (8) for lifting the second block (7) is installed inside the housing (2). A stepped groove is opened at the top edge of the filter box (1), and a matching filter frame (9) is provided in the stepped groove. A matching filter screen (10) is installed in the filter frame (9). The counterweight assembly includes fixed pulleys (11) symmetrically installed on the top of the housing (2), guide wheels (12) installed on the top of the housing (2) near both sides, a hoisting rope (13) connected to the inclined plate (4), a rope hole for the hoisting rope (13) to pass through the top of the housing (2), and the end of the hoisting rope (13) away from the inclined plate (4) passing around the fixed pulley (11) and the guide wheel (12) in sequence and being connected to a counterweight block (14). The counterweight block (14) is slidably connected to the outer wall of the housing (2). A pressure frame (21) is provided above the filter frame (9). An isolation frame (22) is fixedly connected to the top of the pressure frame (21), and a sealing pressure pad (23) that fits against the edge of the top of the filter frame (9) is connected to the bottom of the pressure frame (21). The isolation frame (22) is fixedly installed on the inner wall of the housing (2) near the bottom. The two ends of the bottom of the T-shaped rod (8) are fixedly connected to the inner walls of the two sides of the isolation frame (22).

2. The culture medium filtration device according to claim 1, characterized in that, The elastic component includes a cylinder (15) symmetrically installed at the bottom of the partition (5), a piston (16) is slidably installed inside the cylinder (15), a slide rod (17) connected to the bottom of the piston (16) and connected to the second block (7), and a spring (18) is installed on the upper surface inside the cylinder (15), the bottom end of the spring (18) is connected to the top of the piston (16).

3. The culture medium filtration device according to claim 1, characterized in that, The bottom of the housing (2) has a rectangular hole corresponding to the filter box (1). The filter box (1) has guide blocks (19) installed at the four corners near the top. The guide blocks (19) have guide holes. The bottom of the housing (2) has bolts that pass through the guide holes, and the bolts are threaded with matching locking nuts (20).

4. A culture medium filtration device according to claim 1, characterized in that, A matching sealing ring is installed between the hoisting rope (13) and the rope hole.

5. A culture medium filtration device according to claim 1, characterized in that, The inner diameter of the top end of the first conical hole, the second conical hole and the third conical hole is larger than the inner diameter of the bottom end, and rubber pads are installed in the first conical hole, the second conical hole and the third conical hole respectively. The filter box (1) is connected to the discharge pipe (24) near the bottom side wall.

6. A culture medium filtration device according to claim 1, characterized in that, The cylindrical part (302) has an external thread on the outer side wall near the top. The cover (301) is threadedly connected to the cylindrical part (302). Two symmetrical inverted L-shaped rods (25) are rotatably installed on the top of the housing (2). A threaded rod (26) is provided through the horizontal part of the inverted L-shaped rod (25). A pressure block for applying pressure to the cover (301) is installed at the bottom of the threaded rod (26).

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