Piercing assembly, detector and detection system

Abstract

The piercing assembly provided in the application comprises a piercing device with a piercing mechanism, and further comprises at least one of a rotating valve, a pushing apart piece and a communication nozzle, wherein the rotating valve is arranged on the piercing mechanism and connected with a rotor of a switching valve of a microfluidic chip during movement of the piercing mechanism to drive the rotor to rotate and realize switching of a valve position of the switching valve; the pushing apart piece is arranged on the piercing mechanism and separates two temperature control modules of a temperature control device during movement of the piercing mechanism, so that a part to be temperature controlled of the microfluidic chip enters and exits between the two temperature control modules; and the communication nozzle is arranged on the piercing mechanism and connected with a communication port of the microfluidic chip during movement of the piercing mechanism to communicate the communication port with a driving pump. Based on this, the performance of the detection instrument can be improved.

Description

Technical Field

[0001] The present application relates to the field of microfluidic detection technology, and in particular to a puncture component, a detector and a detection system. Background Art

[0002] A microfluidic chip is a microfluidic chip that integrates multiple experimental steps. It is generally equipped with tiny flow channels and chambers arranged in a certain pattern. Different reagents are released in a certain order and flow into designated chambers through different flow channels to complete designated biochemical reactions, thereby achieving purposes such as sample preparation and detection.

[0003] Detectors are used to automatically manipulate microfluidic chips to control the preparation and detection of targets such as nucleic acids. The performance of related detectors still needs to be improved. Summary of the Invention

[0004] A technical problem to be solved by this application is to improve the performance of the detector.

[0005] In order to solve the above technical problems, the first aspect of the present application provides a piercing assembly, which includes:

[0006] The puncture device includes a puncture support, a puncture mechanism, and a puncture drive mechanism. The puncture mechanism is movably mounted on the puncture support. The puncture drive mechanism is drivably connected to the puncture mechanism to drive the puncture mechanism to move relative to the puncture support. During the movement of the puncture mechanism relative to the puncture support, the puncture needle is driven to move relative to the storage cavity of the microfluidic chip mounted on the holding device, thereby puncturing the sealing membrane on the storage cavity.

[0007] Furthermore, the piercing assembly further comprises at least one of the following:

[0008] The rotary valve member is provided on the piercing mechanism and is connected to the rotor of the switching valve of the microfluidic chip during the movement of the piercing mechanism to drive the rotor to rotate and realize the switching of the valve position of the switching valve;

[0009] The pushing member is provided on the piercing mechanism and separates the two temperature control modules of the temperature control device as the piercing mechanism moves, so that the part to be temperature-controlled of the microfluidic chip moves in and out between the two temperature control modules;

[0010] The communication nozzle is arranged on the piercing mechanism and is connected to the communication port of the microfluidic chip during the movement of the piercing mechanism to connect the communication port with the driving pump.

[0011] In some embodiments, the rotary valve member includes a rotary valve shaft, which is used to be sleeved outside the valve stem of the rotor and realize the switching of the valve position of the switching valve by driving the valve stem to rotate.

[0012] In some embodiments, the inner hole of the valve shaft for cooperating with the valve rod is a polygonal hole.

[0013] In some embodiments, the piercing mechanism comprises a piercing member which, during relative movement of the piercing support, acts on the microfluidic chip so that the piercing needle moves relative to the storage cavity, and the piercing member is sleeved outside the valve member.

[0014] In some embodiments, the end of the valve member close to the holding device protrudes from the end of the piercing member close to the holding device.

[0015] In some embodiments, the distance between the end of the valve member close to the holding device and the end of the piercing member close to the holding device is less than the distance between the top end of the rotor and the plane where the connecting rib of the microfluidic chip is located.

[0016] In some embodiments, the piercing assembly comprises a valve driving mechanism which is drivingly connected with the valve member to drive the valve member to rotate the rotor.

[0017] In some embodiments, the valve driving mechanism is arranged on the piercing mechanism.

[0018] In some embodiments, the poking member is rotatably arranged on the piercing mechanism.

[0019] In some embodiments, the piercing assembly comprises a constraint member which supports the communicating mouth and a resilient member which is sleeved outside the communicating mouth and abuts against the constraint member to apply a resilient force to the communicating mouth towards the communicating port.

[0020] In some embodiments, the piercing mechanism causes the piercing needle to pierce the sealing film on the storage cavity by pressing the connecting rib of the microfluidic chip connecting the piercing needle to break.

[0021] The second aspect of the present application provides a detection instrument which comprises a holding device for holding a microfluidic chip, and further comprises the piercing assembly of the embodiments of the present application, and the piercing mechanism of the piercing assembly is movably arranged relative to the holding device.

[0022] In some embodiments, the holding device is horizontally movably arranged, and the piercing mechanism is vertically movably arranged.

[0023] In some embodiments, the detection instrument comprises a temperature control device which comprises two temperature control modules which are opposite to each other and are relatively movable, and the two temperature control modules control the temperature of the part to be controlled when the part to be controlled is inserted between the two temperature control modules, and the poking member separates the two temperature control modules.

[0024] In some embodiments, the temperature control device comprises two openers, the two openers are connected with the two temperature control modules one by one, and the opening mechanism pushes the two openers away from each other to separate the two temperature control modules.

[0025] In some embodiments, the opener comprises a convex part, and the opening mechanism pushes the two openers away from each other by acting on the convex part.

[0026] In some embodiments, the surface of the opener for contacting with the opening mechanism is arc-shaped.

[0027] In some embodiments, the temperature control device comprises a reset elastic member, the reset elastic member abuts against the opener and applies an elastic force on the opener to make the two openers close to each other.

[0028] The third aspect of the present application provides a detection system, which comprises a microfluidic chip and further comprises the detection instrument of the embodiments of the present application.

[0029] In some embodiments, along the direction of the rotating valve shaft of the detection instrument approaching the holding device, the cross-sectional size of the inner hole of the rotating valve shaft is unchanged, and the cross-sectional size of the valve rod of the microfluidic chip is increased; or, the cross-sectional size of the valve rod of the microfluidic chip is unchanged, and the cross-sectional size of the inner hole of the rotating valve shaft is decreased, so that the connection tightness between the rotating valve shaft and the valve rod is increased along the direction of the rotating valve shaft approaching the holding device.

[0030] By arranging at least one of the rotating valve member, the opening member and the communication nozzle on the piercing mechanism 32, the structure of the detection instrument is simplified, the volume of the detection instrument is reduced, the working efficiency and the use safety of the detection instrument are improved, and the performance of the detection instrument is further improved.

[0031] Other features and advantages of the present application will become apparent from the following detailed description of exemplary embodiments thereof, taken in conjunction with the accompanying drawings. BRIEF DESCRIPTION OF DRAWINGS

[0032] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed to be used in the embodiments or the prior art description will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without creative labor.

[0033] Figure 1 It is a perspective view of the detection system in the embodiments of the present application.

[0034] Figure 2 It is a perspective view of the microfluidic chip in the embodiments of the present application.

[0035] Figure 3An exploded view of a microfluidic chip in an embodiment of the present application.

[0036] Figure 4 A combined view of the body and rotor of a microfluidic chip in an embodiment of the present application.

[0037] Figure 5 A perspective view of a detector in an embodiment of the present application.

[0038] Figure 6 A first perspective view of a piercing assembly in an embodiment of the present application.

[0039] Figure 7 A second perspective view of a piercing assembly in an embodiment of the present application.

[0040] Figure 8 A third perspective view of a piercing assembly in an embodiment of the present application.

[0041] Figure 9 A structure of a piercing device in an embodiment of the present application is shown.

[0042] Figure 10 An installation view of a transfer valve mechanism on a piercing mechanism in an embodiment of the present application is shown.

[0043] Figure 11 A structure of a communication device in an embodiment of the present application is shown.

[0044] Figure 12 An installation view of a communication device on a piercing mechanism in an embodiment of the present application is shown.

[0045] Figure 13 A structure of a temperature control device in an embodiment of the present application is shown.

[0046] Figure 14 A structure of a temperature control unit in an embodiment of the present application is shown.

[0047] Figure 15 A state view of two temperature control modules being separated by a separating mechanism in an embodiment of the present application is shown.

[0048] Figure 16 A state view of two temperature control modules not being separated by a separating mechanism in an embodiment of the present application is shown.

[0049] BRIEF DESCRIPTION OF THE DRAWINGS

[0050] 100, a detection system;

[0051] 10, a detector; 101, a piercing assembly;

[0052] 1, a base;

[0053] 2, a holding device;

[0054] 3, piercing device; 31, piercing support; 311, first vertical plate; 312, second vertical plate; 313, horizontal plate; 32, piercing mechanism; 321, support plate; 322, piercing member; 323, piercing slider; 324, piercing guide rail; 325, avoiding opening; 326, first groove part; 33, piercing driving mechanism; 331, piercing motor; 332, piercing gear; 333, piercing rack; 34, piercing detection device; 341, first piercing detection member; 342, second piercing detection member; 343, piercing trigger member; 345, opening detection mechanism; 35, pushing away mechanism; 351, pushing away member; 352, rotating bearing; 353, connecting shaft;

[0055] 4, rotating valve device; 41, rotating valve member; 411, rotating valve shaft; 42, rotating valve support; 43, rotating valve driving mechanism; 431, rotating valve motor; 432, rotating valve transmission mechanism; 433, belt transmission mechanism; 434, pulley; 436, belt; 437, tensioning member; 44, angle detection device; 441, angle detection member; 442, Hall sensor; 443, mounting seat; 446, magnet seat;

[0056] 5, pump assembly; 51, driving pump; 52, communication nozzle; 521, limiting step; 53, clamp; 532, second groove part; 54, constraint member; 55, elastic member; 551, spring; 56, guide bearing; 5a, communication device;

[0057] 6, temperature control device; 61, temperature control module; 611, heat exchange device; 611a, semiconductor refrigeration sheet; 611b, heat transfer sheet; 611c, first positioning member; 611d, second positioning member; 612, sliding assembly; 612a, temperature control elastic member; 612b, support shaft; 612c, sliding guide mechanism; 613, heat dissipation device; 613a, heat dissipation block; 613b, heat dissipation fan; 613c, heat dissipation block fixing member; 613d, connecting block; 62, opening mechanism; 621, opening member; 622, convex part; 623, reset elastic member; 624, shaft body; 63, supporting plate; 64, pulling plate; 65, supporting plate; 66, temperature control unit;

[0058] 7, ultrasonic device;

[0059] 8, magnetic attraction device;

[0060] 91, detection device; 911, excitation optical fiber; 912, emission optical fiber;

[0061] 20, microfluidic chip; 201, body; 201a, storage cavity; 201b, mounting groove; 202, base; 202a, reaction cavity; 202b, positioning groove; 203, switching valve; 203a, rotor; 203b, valve stem; 203c, valve seat; 203d, seat body; 203e, gasket; 203f, valve inner flow channel; 203g, valve cover; 204, top cover; 204a, sample inlet; 204b, communication port; 204c, piercing needle; 204d, connecting rib; 204e, stopper; 204f, rib; 204g, cover body; 204h, annular member; 204j, airway; 205, amplification member; 205a, amplification cavity; 206, cover sheet; 206a, through hole; 207, temperature control part. DETAILED DESCRIPTION

[0062] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments of the present application. The following description of at least one exemplary embodiment is merely illustrative in nature and in no way limits the present application and its applications or uses. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the scope of the present application.

[0063] The technologies, methods and devices known to those of ordinary skill in the relevant art can not be discussed in detail, but should be considered as part of the specification when appropriate.

[0064] In the description of the present application, it should be understood that the use of the words "first", "second" and the like to describe components is merely intended to facilitate the differentiation between the corresponding components, and the above words do not have special meanings unless otherwise stated, and therefore should not be understood as limiting the scope of the present application.

[0065] In addition, the technical features involved in different embodiments of the present application described below can be combined with each other as long as there is no conflict.

[0066] Figures 1-16 The detection system 100 of the present application and its microfluidic chip 20 and detection instrument 10 are exemplarily shown.

[0067] Reference Figures 1-16 In the present application, the detection system 100 includes a microfluidic chip 20 and a detection instrument 10. The detection instrument 10 is used to clamp and fix the microfluidic chip 20 and control it, so as to realize automatic control of the microfluidic detection process.

[0068] For the convenience of understanding, first, the microfluidic chip 20 will be described in combination with Figures 2-4The structure of the microfluidic chip 20 is introduced. Moreover, although the microfluidic chip 20 of the present application can be used to implement various detection processes similar to nucleic acid detection, the following mainly takes the case of nucleic acid detection as an example for description to simplify the description. Among them, the nucleic acid detection process generally includes nucleic acid extraction, nucleic acid amplification and nucleic acid detection, etc. Specifically, the nucleic acid is extracted from the sample (such as whole blood or serum) containing cells through lysis and purification steps, and then the extracted nucleic acid is amplified, and the target nucleic acid is detected during the amplification process.

[0069] Referring to Figures 2-4 In some embodiments, the microfluidic chip 20 includes a body 201, a top cover 204, a cover sheet 206, a base 202, an amplification piece 205 and a switching valve 203.

[0070] Among them, the body 201 is used to store various fluids required for nucleic acid detection experiments such as samples and lysis solutions. Referring to Figure 4 The body 201 is provided with a storage cavity 201a for storing the required fluid. Specifically, as Figure 4 shown, in some embodiments, the body 201 is provided with a plurality of storage cavities 201a, which are arranged side by side for storing different fluids, for example, a part of the storage cavities 201a are used to store samples such as whole blood, which can be referred to as sample cavities, and another part of the storage cavities 201a are used to store reagents (such as various reaction reagents required for preparing nucleic acid from samples and auxiliary reagents such as magnetic beads, washing solution and eluent), which can be referred to as reagent cavities. More specifically, as Figure 4 shown, in some embodiments, the body 201 is cylindrical, and all the storage cavities 201a are arranged at the same circumference of the body 201 with a spacing between each other.

[0071] The end of the storage cavity 201a is provided with a sealing film (not shown in the figure) to close the end opening of the storage cavity 201a, so as to realize the sealing of the fluid in the storage cavity 201a, effectively prevent the fluid from leaking or spilling accidentally, so as to facilitate the transportation of the microfluidic chip 20, and facilitate the control of making the fluid flow out only when needed, improve the controllability and safety. Specifically, as Figure 4 shown, in some embodiments, the storage cavity 201a is provided with a sealing film at one end only, and the other end is directly closed by the end wall of the body 201, at this time, the storage cavity 201a is a groove with a groove bottom.

[0072] When the sealing film is pierced, the storage cavity 201a is in communication with the outside atmosphere, and the fluid in the storage cavity 201a can flow out to the outside of the storage cavity 201a.

[0073] The piercing of the sealing film is implemented by a piercing needle 204c. In some embodiments, the piercing needle 204c is arranged on the top cover 204.

[0074] The top cover 204 is used to provide a mounting base for the piercing needle 204c. As shown in Figure 2 and Figure 3 The top cover 204 covers the end of the body 201, and a piercing needle 204c is arranged on the wall surface thereof facing the body 201. The piercing needle 204c corresponds to the storage cavity 201a one-to-one, and is connected to the top cover 204 through a connecting rib 204d. In the initial state, there is a gap between the piercing needle 204c and the sealing film, and the two are not in contact. When the connecting rib 204d is broken, the piercing needle 204c falls off from the top cover 204 and moves towards the storage cavity 201a, and the sealing film in the storage cavity 201a is pierced, so that the storage cavity 201a is in communication with the atmosphere.

[0075] Specifically, as shown in Figures 2-4 , in some embodiments, the top cover 204 includes a cover body 204g and a ring-shaped member 204h.

[0076] The cover body 204g covers the top of the body 201 and is clamped with the body 201 to achieve the connection and fixation of the top cover 204 and the body 201. The cover body 204g is provided with a communication port 204b. The communication port 204b is used to communicate with the driving pump 51, so that the driving pump 51 drives the fluid to flow between different chambers of the microfluidic chip 20, realizing the transfer of the fluid.

[0077] The outer edge of the ring-shaped member 204h is connected to the top wall of the cover body 204 through a plurality of connecting ribs 204d, and the inner edge of the ring-shaped member 204h is connected to a stopper 204e through a plurality of ribs 204f. The stopper 204e is generally ring-shaped and extends from the ring-shaped member 204h towards the body 201. The ring-shaped member 204h is provided with a sample addition port 204a, which communicates with one of the plurality of storage cavities 201a (i.e. a sample cavity) to add a sample to be detected into the corresponding storage cavity 201a.

[0078] The surface of the ring-shaped member 204h facing the body 201 is provided with a piercing needle 204c. The piercing needle 204c is located between the outer edge of the ring-shaped member 204h and the stopper 204e in the radial direction, and includes a first needle segment (not marked in the figure) and a second needle segment (not shown in the figure). The radial dimension of the second needle segment is greater than that of the first needle segment. The first needle segment is connected to the ring-shaped member 204h through the second needle segment, and the bottom end thereof is configured to be sharp to pierce the sealing film. The piercing needle 204c is provided with a ventilation air passage 204j extending through the first needle segment and into the second needle segment, and the side wall of the second needle segment is provided with a ventilation hole (not shown in the figure) in communication with the ventilation air passage 204j, so that the piercing needle 204c can communicate the storage cavity 201a with the external atmosphere after piercing the sealing film.

[0079] The connecting rib 204d and the rib 204f can be broken to achieve the separation between the ring 204h and the cover 204, and between the stopper 204e and the ring 204, to facilitate the piercing needle 204c to pierce the sealing film before the extraction starts and to cooperate with the cover sheet 6 to reseal the storage cavity 201a after the extraction ends.

[0080] The cover sheet 206 is arranged between the body 201 and the top wall of the top cover 204, and has a through hole 206a allowing the piercing needle 204c to pass through. The diameter of the through hole 206a is larger than the outer diameter of the first needle segment and slightly smaller than the outer diameter of the second needle segment. When the second needle segment is inserted into the through hole 206a under the action of an external force, the second needle segment is in interference fit with the through hole 206a, and in this case, the vent hole on the second needle segment is blocked by the cover sheet 206, so that the storage cavity 201a can be resealed to prevent leakage of waste liquid and pollution.

[0081] In use of the microfluidic chip 20, pressure is applied to the ring 204h on the top cover 204 to break the connecting rib 204d, and the ring 204h drives each piercing needle 204c to separate from the cover 204g and press against the sealing film on the storage cavity 201a, so that the piercing needle 204c pierces the sealing film. After the piercing needle 204c pierces the sealing film, the stopper 204e abuts against the body 201 to prevent the piercing needle 204c from moving downward excessively, and the gas in the storage cavity 201a is communicated with the atmosphere through the piercing needle 204c. After the extraction step is completed, the pressure continues to be applied to the ring 204h, and since the stopper 204e is prevented from moving downward further by the body 201, when the pressure reaches a certain level, the pressure will break the rib 204f connected between the ring 204h and the stopper 204e, so that the ring 204h separates from the stopper 204e. At this time, the stopper 204e no longer prevents the piercing needle 204c from moving downward, and therefore the ring 204h and each piercing needle 204c can further press against the sealing film under the action of an external force, until the second needle segment is in interference fit with the through hole on the cover sheet 206, and the cover sheet 206 blocks the vent hole on the second needle segment to reseal the storage cavity 201a.

[0082] In summary, the piercing needle 204c on the top cover 204 is used to pierce the sealing film on the storage cavity 201a to communicate the storage cavity 201a with the atmosphere to provide conditions for the fluid flow in the storage cavity 201a. The stopper 204e on the top cover 204 is used to cooperate with the body 201 to keep the piercing needle 204c in the piercing position before the extraction step ends to communicate the storage cavity 201a with the atmosphere without excessive downward movement, and to cooperate with the piercing mechanism 32 to break the rib 204f after the extraction ends. The cover sheet 206 is used to cooperate with the piercing needle 204c to seal the storage cavity 201a after the extraction step ends to prevent leakage of waste liquid.

[0083] The base 202 is disposed on a side of the body 201 away from the top cover 204 and is used to receive the fluid flowing out of the storage chamber 201a to complete the extraction of target substances such as nucleic acids. Figure 3 As shown, the base 202 is provided with a reaction chamber 202a. Reaction chamber 202a serves as the location for extracting and preparing the target substance. Samples and reagents are sequentially introduced into reaction chamber 202, where reactions occur to produce the target substance. Reaction chamber 202a is located at the bottom of base 202 and protrudes downward, forming a spherical crown. This arrangement allows reaction chamber 202a to couple with the ultrasonic device 7 and magnetic device 8 of the detector 10, described below.

[0084] In addition, if Figure 3 As shown, a positioning groove 202 b is provided on the base 202 . The positioning groove 202 b is used to engage with the holding device 2 of the detector 10 to achieve installation of the microfluidic chip 20 on the detector 10 .

[0085] The amplification element 205 is connected to one side of the body 201 and extends outward to provide a place for amplification of the target object to meet the detection requirements of the target object such as nucleic acid that needs to be amplified before detection. Figure 2 and Figure 3 As shown, in some embodiments, the amplification element 205 is removably connected to the side of the body 201 and has an amplification chamber 205a disposed therein. The amplification chamber 205a is where the amplification reaction occurs. Targets, such as nucleic acids, prepared in the reaction chamber 202a are delivered to the amplification chamber 205a and amplified there for detection.

[0086] like Figure 2 and Figure 3 As shown, in some embodiments, the amplification component 205 is in the shape of a thin sheet as a whole, so that the amplification component 205 can fully contact and exchange heat with the temperature control device 6 of the detector 10 mentioned below, thereby achieving a rapid temperature increase and decrease process.

[0087] The switching valve 203 is arranged on the main body 201 and is used to control the on-off relationship between the chambers of the microfluidic chip 20 (such as the storage chamber 201a, the reaction chamber 202a and the amplification chamber 205a), so as to control the fluid in the microfluidic chip 20 to flow in the required order, thereby successfully completing the extraction and amplification steps.

[0088] like Figures 2-4 As shown, in some embodiments, a mounting groove 201 b is provided at the center of the body 201 , and the switching valve 203 is disposed in the mounting groove 201 b and includes a rotor 203 a and a valve cover 203 g.

[0089] The valve cover 203g is connected to the circumferential sidewall of the mounting groove 201b. The rotor 203a is rotatably mounted within the mounting groove 201b and comprises a seat 203d, a valve stem 203b, and a gasket 203e. The gasket 203e and the seat 203d have the same circular bottom shape. The seat 203d and gasket 203e are fixedly mounted therebetween. The combined structure of the seat 203d and gasket 203e defines an internal valve flow channel 203f. The radial dimension of the valve stem 203b is smaller than the radial dimension of the seat body 203d, and one end of the valve stem 203b is fixedly connected to the seat body 203d, and the other end passes through the valve cover 203g, the cover plate 206 and the top cover 204, and is used to be connected to the rotary valve device 4 of the detector 10 mentioned below, so that it can rotate under the drive of the rotary valve device 4, so that the flow channel 203f in the valve is connected to different chambers of the microfluidic chip 20, thereby realizing the switching of the valve position of the switching valve 203.

[0090] Rotating valve assembly 4 rotates valve stem 203b, driving seat 203d and gasket 203e to rotate, causing valve channel 203f to change position, switching between connecting reaction chamber 202a and different chambers, thereby completing the flow transfer during the detection process. For example, when valve channel 203f connects reaction chamber 202a to different storage chambers 201a, samples and reagents in storage chamber 201a can flow sequentially into reaction chamber 202a under the action of external force, completing the extraction process. For another example, when valve channel 20f connects reaction chamber 202a to amplification chamber 205a, the target prepared in reaction chamber 202a can flow into amplification chamber 205a under the action of external force for amplification.

[0091] Among them, Figure 3 and Figure 4 It can be seen that in some embodiments, the outer surface of the valve stem 203b is a polygonal surface (for example, a hexagonal surface). This makes it easier to achieve a non-rotatable connection between the valve stem 203b and the rotary valve member 41 of the rotary valve device 4, thereby facilitating the rotary valve device 4 to reliably drive the valve stem 203b to rotate.

[0092] It is understood that the structure of the microfluidic chip 20 is not limited to Figures 2-4 For example, in some other embodiments, the microfluidic chip 20 may not be Figures 2-4 Similarly, the main body part (the part except the amplification part 205) is cylindrical, but the main body part is also flat. As an example, the flat microfluidic chip 20 can refer to the applicant's prior patent application CN111135892A.

[0093] Based on the above introduction to the structure of the microfluidic chip 20 , the structure of the detector 10 will be introduced next.

[0094] Figures 5-16 The structure of the detector 10 is shown as an example.

[0095] Referring to Figures 5-16 , in order to realize the automatic operation of the whole process of the microfluidic detection process based on the microfluidic chip 20, in some embodiments, the detector 10 comprises a holding device 2, a piercing device 3, a rotary valve device 4, a pump assembly 5, a temperature control device 6, a dislodging piece 351, an ultrasonic device 7, a magnetic attraction device 8 and a detection device 91.

[0096] The holding device 2 is used to hold the microfluidic chip 20 to realize the installation and fixation of the microfluidic chip 20 on the detector 10, so as to facilitate the operation of the microfluidic chip 20 by other modules of the detector 10.

[0097] The piercing device 3 is used to control the piercing of the sealing film on the storage bin 201a of the microfluidic chip 20 to provide conditions for the flow of fluid in the microfluidic chip 20.

[0098] The rotary valve device 4 is used to drive the rotor 203a of the switching valve 203 of the microfluidic chip 20 to rotate to realize the switching of the valve position of the switching valve 203, so that the fluid in the microfluidic chip 20 can flow in sequence. As Figures 6-10 shown, in some embodiments, the rotary valve device 4 comprises a rotary valve piece 41 and a rotary valve driving mechanism 43, the rotary valve piece 41 is detachably connected with the rotor 203a of the switching valve 203, and the rotary valve driving mechanism 43 is drivingly connected with the rotary valve piece 41 to drive the rotor 203a to rotate when the rotary valve piece 41 is connected with the rotor 203a, thereby realizing the switching of the valve position of the switching valve 203.

[0099] The pump assembly 5 is used to communicate with the communication port 204b of the microfluidic chip 20 and drive the fluid flow in the microfluidic chip 20. As Figures 5-12 shown, in some embodiments, the pump assembly 5 comprises a communication nozzle 52 and a driving pump 51. The communication nozzle 52 is detachably connected with the communication port 204b of the microfluidic chip 20. The driving pump 51 communicates with the communication nozzle 52 to drive the fluid flow in the microfluidic chip 20 when the communication nozzle 52 is connected with the communication port 204b.

[0100] The temperature control device 6 is used to control the temperature of the temperature control part 207 of the microfluidic chip 20 to meet the temperature requirement in the microfluidic detection process. As Figures 13-16As shown, in some embodiments, the temperature control device 6 includes two temperature control modules 61. The two temperature control modules 61 are opposite to each other and are movable relative to each other. The two temperature control modules 61 control the temperature of the to-be-temperature-controlled part 207 of the microfluidic chip 20 when the to-be-temperature-controlled part 207 is inserted between the two temperature control modules 61. Generally, the to-be-temperature-controlled part 207 includes the amplification cavity 205a. During the operation of the detection instrument 10 on the microfluidic chip 20, the amplification part 205 of the microfluidic chip 20 is inserted between the two temperature control modules 61, so that the amplification cavity 205a is in contact with the two temperature control modules 61, so as to provide suitable temperature conditions for the amplification process by using the two temperature control modules 61 to heat or cool the amplification cavity 205a when amplification is needed.

[0101] The disengaging member 351 is used to separate the two temperature control modules 61 of the temperature control device 6, so as to insert and pull out the to-be-temperature-controlled part 207 of the microfluidic chip 20.

[0102] The ultrasonic device 7 is used to apply ultrasonic excitation to the reaction cavity 202a of the microfluidic chip 20 to assist the extraction process.

[0103] The magnetic attraction device 8 is used to attract magnetic substances (such as magnetic beads) in the reaction cavity 202a of the microfluidic chip 20 to assist the extraction process.

[0104] The detection device 91 is used to detect the target obtained by amplification in the amplification cavity 205a of the microfluidic chip 20. In some embodiments, the detection device 91 is a fluorescence detection device, which emits excitation light to the amplification cavity 205a through the excitation optical fiber 911, excites fluorescence, and recovers the excited fluorescence through the emission optical fiber 912, so as to determine the presence and quantity of the target according to the intensity of the fluorescence excited in the amplification cavity 205a.

[0105] As can be seen, based on the cooperation of the holding device 2, the piercing device 3, the rotary valve device 4, the pump assembly 5, the temperature control device 6, the ultrasonic device 7, the magnetic attraction device 8 and the detection device 91, the detection instrument 10 can complete the extraction, amplification and detection of the target such as nucleic acid, and realize the full-automatic operation of the entire microfluidic detection process.

[0106] In the related art, the detection instrument 10 simultaneously has the piercing device 3, the rotary valve device 4, the pump assembly 5 and the disengaging member 351, and the piercing device 3, the rotary valve device 4, the pump assembly 5 and the disengaging mechanism 35 are separately arranged from each other, and the communication nozzle 52 of the rotary valve device 4, the pump assembly 5 and the disengaging member 351 are not integrated on the piercing device 3. In this case, not only the structure of the detection instrument 10 is relatively complex and the volume is large, but also the working efficiency and the use safety of the detection instrument 10 are affected.

[0107] As can be seen, the performance of the detection instrument 10 in the related art still needs to be improved.

[0108] In order to improve the performance of the detection instrument 10, the present application provides a piercing assembly 101.

[0109] Figures 6-12 The structure of the piercing assembly 101 in the present application is exemplarily shown.

[0110] Referring to Figures 6-12 In the embodiment of the present application, the piercing assembly 101 comprises the piercing device 3, and further comprises at least one of the rotating valve 41, the pusher 351 and the communication nozzle 52.

[0111] The piercing device 3 comprises a piercing support 31, a piercing mechanism 32 and a piercing driving mechanism 33. The piercing mechanism 32 is movably arranged on the piercing support 31. The piercing driving mechanism 33 is drivingly connected with the piercing mechanism 32 to drive the piercing mechanism 32 to move relative to the piercing support 31. During the movement of the piercing mechanism 32 relative to the piercing support 31, the piercing mechanism 32 drives the piercing needle 204c to move relative to the storage cavity 201a of the microfluidic chip 20 installed on the holding device 2, and pierces the sealing film on the storage cavity 201a. As an example, in the case that the piercing needle 204c is arranged in the microfluidic chip 20 and is fixed by the connecting rib 204d, the piercing mechanism 32 breaks the connecting rib 204d of the microfluidic chip 20 connecting the piercing needle 204c to make the piercing needle 204c move relative to the storage cavity 201a, and realizes the piercing of the sealing film on the storage cavity 201a. And in the case that the microfluidic chip 20 has a cover sheet 206 as shown, after the piercing mechanism 32 pierces the sealing film, the piercing mechanism 32 further drives the piercing needle 204c to move towards the holding device 2, so that the piercing needle 204c and the cover sheet 206 of the microfluidic chip 20 together seal the storage cavity 201a, and further realize the resealing of the storage cavity 201a to prevent the leakage of the waste liquid in the storage cavity 201a. Figures 2-4

[0112] The rotating valve 41 is arranged on the piercing mechanism 32, and during the movement of the piercing mechanism 32, the rotating valve 41 connects with the rotor 203a of the switching valve 203 of the microfluidic chip 20 to drive the rotor 203a to rotate and realize the switching of the valve position of the switching valve 203.

[0113] The pusher 351 is arranged on the piercing mechanism 32, and during the movement of the piercing mechanism 32, the pusher 351 separates the two temperature control modules 61 of the temperature control device 6, so that the temperature control part 207 of the microfluidic chip 20 enters and exits between the two temperature control modules 61.

[0114] ​The communication nozzle 52 is arranged on the puncture mechanism 32 and is connected with the communication port 204b of the microfluidic chip 20 during movement of the puncture mechanism 32, so as to communicate the communication port 204b with the driving pump.

[0115] By arranging at least one of the rotary valve 41, the disengaging piece 351 and the communication nozzle 52 on the puncture mechanism 32, the structure of the detection instrument 10 is simplified, the volume of the detection instrument 10 is reduced, the working efficiency and use safety of the detection instrument 10 are improved, and the performance of the detection instrument 10 is further improved.

[0116] Next, the rotary valve 41, the disengaging piece 351 and the communication nozzle 52 are described respectively.

[0117] First, the disengaging piece 351 is described.

[0118] In the related art, the disengaging piece 351 is not integrated on the puncture device 3, but is arranged independently of the puncture device 3. For example, in some related art, the disengaging piece 351 is arranged on the holding device 2 and is not movable relative to the holding device 2. During work, the disengaging piece 351 is driven by the holding device 2 to approach the temperature control device 6 together with the microfluidic chip 20, and is inserted between the two temperature control modules 61 before the temperature control part 207 of the microfluidic chip 20, so as to open the two temperature control modules 61, so that the disengaging piece 351 can open the two temperature control modules 61 before the temperature control part 207 is inserted between the two temperature control modules 61, reduce the insertion resistance of the temperature control part 207, and enable the temperature control part 207 to be smoothly inserted between the two temperature control modules 61.

[0119] In the above related art, although the disengaging piece 351 can open the two temperature control modules 61 before the temperature control part 207 is inserted between the two temperature control modules 61, facilitating the insertion of the temperature control part 207, research has found that since the disengaging piece 351 is arranged immovably on the holding device 2, the disengaging piece 351 inserted between the two temperature control modules 61 before the temperature control part 207 can only separate the two temperature control modules 61 after the temperature control part 207 moves outward by a distance, that is, during the initial stage of the extraction process of the temperature control part 207, the disengaging piece 351 has not yet separated the two temperature control modules 61. Since the two temperature control modules 61 that have not been separated will rub against the outwardly moving temperature control part 207, not only does this increase the difficulty of extraction, but it also easily damages the temperature control part 207, causing the fluid in the temperature control part 207 to flow out unexpectedly, resulting in pollution and affecting the normal use of the detection instrument 10.

[0120] It can be seen that the setting mode of the dislodging piece 351 in the related technology is convenient for the insertion of the to-be-temperature-controlled part 207, but is not convenient for the extraction of the to-be-temperature-controlled part 207, and is likely to cause the to-be-temperature-controlled part 207 to be abraded and leaked during the extraction process, so that the detector 10 is difficult to safely and reliably control the microfluidic chip 20, and the performance of the detector 10 is affected.

[0121] Unlike the related technology, in the embodiment of the present application, the dislodging piece 351 is no longer movably arranged on the holding device 2, but is arranged on the piercing mechanism 32 of the piercing device 3 and moves together with the piercing mechanism 32, so that the two temperature control modules 61 can be conveniently opened before the to-be-temperature-controlled part 207 moves outward, reducing the abrasion of the to-be-temperature-controlled part 207 during the extraction process and reducing the risk of leakage of the to-be-temperature-controlled part 207 during the extraction process.

[0122] Specifically, after the detection is completed, the piercing mechanism 32 will act before the holding device 2 to release the constraint of the piercing mechanism 32 on the microfluidic chip 20 on the holding device 2, and then facilitate the microfluidic chip 20 to leave the detector 10 (i.e. out of the warehouse) under the action of the holding device 2. Therefore, arranging the dislodging piece 351 on the piercing mechanism 32 can conveniently use the feature that the piercing mechanism 32 moves before the microfluidic chip 20 leaves the warehouse to realize the separation of the dislodging piece 351 from the two temperature control modules 61, so that the dislodging piece 351 can separate the two temperature control modules 61 before the to-be-temperature-controlled part 207 starts to move outward from between the two temperature control modules 61, facilitating the extraction of the to-be-temperature-controlled part 207 and reducing the abrasion of the to-be-temperature-controlled part 207 during the extraction process.

[0123] It can be seen that by arranging the dislodging piece 351 on the piercing mechanism 32 of the piercing device 3, the pre-opening of the two temperature control modules 61 before the to-be-temperature-controlled part 207 moves out can be conveniently realized, effectively reducing the risk of abrasion and leakage of the to-be-temperature-controlled part 207 during the extraction process, and improving the use safety of the detector 10.

[0124] In addition, arranging the dislodging piece 351 on the piercing mechanism 32 of the piercing device 3 also facilitates the opening of the two temperature control modules 61 before the to-be-temperature-controlled part 207 is inserted, reducing the abrasion of the to-be-temperature-controlled part 207 during the insertion process. Specifically, only by controlling the piercing mechanism 32 to drive the dislodging piece 351 to move to the opening position (i.e. the position at which the dislodging piece 351 separates the two temperature control modules 61) before the to-be-temperature-controlled part 207 is inserted, the pre-opening of the two temperature control modules 61 before the to-be-temperature-controlled part 207 is inserted can be realized.

[0125] It can be seen that by arranging the prying member 351 on the piercing mechanism 32, the extraction and insertion of the to-be-temperature-controlled part 207 are facilitated, the wear of the to-be-temperature-controlled part 207 can be more reliably reduced, and the use safety of the detector 10 is improved.

[0126] In addition, by arranging the prying member 351 on the piercing mechanism 32, the prying member 351 moves together with the piercing mechanism 32 under the driving of the piercing driving mechanism 33, and the prying member 351 does not need to be separately provided with a driving mechanism to move relative to the holding device 2, so that the structure is relatively simple and the control is relatively convenient. At the same time, since the prying action can be completed during the movement of the piercing mechanism 32, no additional time is occupied, so that the operation time of the detector 10 on the microfluidic chip 20 is shortened, and the working efficiency of the detector 10 is improved.

[0127] It can be seen that by arranging the prying member 351 on the piercing mechanism 32 and coupling the prying function and the piercing function together, the pre-opening of the two temperature control modules 61 before the to-be-temperature-controlled part 207 is actuated can be efficiently controlled based on a relatively simple structure and a relatively simple control process, and the risk of wear and leakage of the to-be-temperature-controlled part 207 is effectively reduced.

[0128] From the above analysis, it can be seen that by integrating the prying member 351 on the piercing device 3 and arranging the prying member 351 on the piercing mechanism 32, the performance of the detector 10 can be effectively improved.

[0129] In order to facilitate the prying member 351 to open the two temperature control modules 61, refer to Figure 8 In some embodiments, the prying member 351 is rotatably arranged.

[0130] Since the prying member 351 can rotate, the friction is small when it acts on the temperature control device 6, which is beneficial to reduce the friction between the prying mechanism 35 and the temperature control device 6 during the movement of the prying mechanism 35 relative to the temperature control device 6, so that the prying mechanism 35 can more smoothly reach the opening position to separate the two temperature control modules 61. In addition, the small friction force is also beneficial to reduce the wear during the interaction between the prying member 351 and the temperature control device 6, and prolong the service life of the prying member 351 and the temperature control device 6.

[0131] As an example of the rotatably arranged prying member 351, refer to Figure 8 The prying member 351 includes a rotating bearing 352. Since the rotating bearing 352 can rotate smoothly, the friction is smaller and the effect is better.

[0132] In addition, refer to Figure 7In some embodiments, the detector 10 comprises an opening detection mechanism 345, which detects whether the disengaging member 351 reaches the opening position. When the disengaging member 351 reaches the opening position, the disengaging member 351 separates the two temperature control modules 61.

[0133] Based on the opening detection mechanism 345, it can be more timely and accurate to determine whether the disengaging member 351 has reached the opening position, and thus it can be more timely and accurate to determine whether the two temperature control modules 61 have been separated, which facilitates the control of the action of the holding device 2. For example, after the detection is completed, the microfluidic chip 20 can be controlled to move outward from between the two temperature control modules 61 after the opening detection mechanism 345 detects that the disengaging member 351 has reached the opening position, so as to reduce the wear of the temperature control part 207 during the extraction process.

[0134] Meanwhile, in the case where the disengaging member 351 is arranged on the piercing mechanism 32, the detection signal of the opening detection mechanism 345 can also be used as a basis for controlling the action of the piercing mechanism 32. For example, the distance from the opening position to the position where the connecting rib 204d is pressed off can be calculated when the opening detection mechanism 345 detects that the disengaging member 351 reaches the opening position, and the required number of steps of the piercing motor 331 in the piercing driving mechanism 33 can be determined based on the calculated distance, so as to facilitate the control of the piercing mechanism 32 to accurately reach the position where the connecting rib 204d is pressed off by controlling the piercing motor 331 to rotate for the corresponding number of steps. In this way, the implementation of the piercing function can be facilitated and accurately controlled, and since there is no need to additionally arrange a special detection component to detect whether the piercing mechanism 32 reaches the position where the connecting rib 204d is pressed off, the structure can also be simplified and the cost can be reduced.

[0135] In addition, referring to Figures 13-16 In order to facilitate cooperation with the disengaging member 351 to separate the two temperature control modules 61, in some embodiments, the temperature control device 6 comprises two opening members 621, which are connected to the two temperature control modules 61 one by one, and the disengaging member 351 separates the two temperature control modules 61 by pushing the two opening members 621 away from each other.

[0136] Since the disengaging member 351 separates the two temperature control modules 61 by pushing the two opening members 621 away from each other, in this case, the disengaging member 351 does not need to act directly on the two temperature control modules 61, for example, the disengaging member 351 does not need to be directly inserted between the two temperature control modules 61 which have almost no gap (fit or only have a small gap), so that the difficulty of separating the two temperature control modules 61 can be effectively reduced, and the wear of the temperature control elements (such as the heat transfer sheet 611b to be mentioned later) in the temperature control module 61 can be avoided, which is conducive to more safely and smoothly achieving the opening function.

[0137] Further, in order to facilitate the cooperation between the opening member 621 and the expelling member 351 to realize the opening function, see Figures 13-16 In some embodiments, the opening member 621 includes a protrusion 622 , and the expelling member 351 acts on the protrusion 622 to push the two opening mechanisms 62 away from each other.

[0138] The provided protrusion 622 makes it easier for the opening member 621 to contact the expelling member 351 , thereby achieving the opening function smoothly.

[0139] Among them, such as Figure 14 As shown, in some embodiments, the surface of the protrusion 622 for contacting the prying member 351 is arc-shaped. In this way, the surface of the opening member 621 for contacting the prying member 351 is arc-shaped, which is beneficial to reducing the friction between the opening member 621 and the prying member 351, and more convenient for the smooth realization of the opening function. In particular, when the prying member 351 is a rotating body and can rotate, the arc-shaped surface of the opening member 621 can better cooperate with the prying member 351 to realize the opening function.

[0140] Also, see Figures 13-16 In some embodiments, the temperature control device 6 includes a reset elastic member 623 , which abuts against the opening member 621 and applies an elastic force to the opening member 621 to move the two opening members 621 closer to each other.

[0141] Based on the reset elastic member 623, when the prying member 351 enters between the two opening members 621 and applies a thrust to the two opening members 621, the reset elastic member 623 can contract so that the two opening members 621 move away from each other, thereby smoothly driving the two temperature control modules 61 to separate from each other. When the prying member 351 no longer applies a thrust to the two opening members 621, the reset elastic member 623 can release the stored elastic force, so that the opening members 621 are reset, and drive the two temperature control modules 61 to reset.

[0142] It can be seen that the reset elastic member 623 can facilitate the automatic reset of the two opening members 621 and the two temperature control modules 61 without affecting the opening function. After the two temperature control modules 61 are reset, they can be in close contact with the temperature-controlled portion 207 inserted between them to achieve a good temperature control effect, or they can wait for the temperature-controlled portion 207 of the next microfluidic chip 20 to be inserted.

[0143] Next, the rotary valve member 41 will be described.

[0144] In the related art, the valve device 4 is arranged independently of the piercing device 3, in which case the valve element 41 occupies a relatively large amount of space, resulting in a relatively large volume of the detection instrument 10, and in this case, the action of the valve element 41 moving close to or away from the holding device 2 and connecting or separating from the rotor 203a of the microfluidic chip 20 needs to be driven by a separate driving mechanism, resulting in a relatively complex structure and a relatively high cost of the detection instrument 10.

[0145] Unlike the above related art, in the embodiments of the present application, the valve element 41 is no longer independent of the piercing device 3, but is arranged on the piercing mechanism 32 and connects or separates from the rotor 203a during movement of the piercing mechanism 32, so that the valve element 41 can be integrated on the piercing device 3, not only making the structure of the detection instrument 10 more compact, but also allowing the valve element 41 to move together with the piercing mechanism 32 under the driving of the piercing driving mechanism 33, so that a separate driving mechanism does not need to be provided for the valve element 41 to drive the valve element 41 to move close to or away from the holding device 2 and connect or separate from the rotor 203a of the microfluidic chip 20 on the holding device 2, thus making the structure simpler and the control more convenient. At the same time, since the valve element 41 can connect or separate from the rotor 203a during movement of the piercing mechanism 32, without the need for additional time, it is also beneficial to shorten the operation time of the detection instrument 10 on the microfluidic chip 20 and improve the working efficiency of the detection instrument 10.

[0146] It can be seen that arranging the valve element 41 on the piercing mechanism 32 can effectively simplify the structure and control process of the detection instrument 10 and improve the working efficiency of the detection instrument 10.

[0147] Therefore, integrating the valve element 41 on the piercing device 3 and arranging it on the piercing mechanism 32 of the piercing device 3 can effectively improve the performance of the detection instrument 10.

[0148] Among them, for the microfluidic chip 20 (see Figures 2-4 ), as shown in Figures 6-10 , the valve element 41 can include a valve shaft 411, which is used to be sleeved outside the valve rod 203b of the rotor 203a and rotate the valve rod 203b to switch the valve position of the valve 203.

[0149] And, continuing to refer to Figures 6-10 , in the case that the valve rod 203b has a polygonal outer surface as shown in Figures 2-4 , the inner hole of the valve shaft 411 for cooperating with the valve rod 203b can be a polygonal hole, which can effectively prevent relative rotation between the valve shaft 411 and the valve rod 203b, and facilitate the valve shaft 411 to rotate the valve rod 203b for valve position switching.

[0150] In addition, referring to Figure 6 and Figure 7 In some embodiments, the piercing mechanism 32 comprises a piercing member 322, which, during the relative movement of the piercing support 31, acts on the microfluidic chip 20 so that the piercing needle 204c moves relative to the storage cavity 201a. At this time, the piercing member 322 is sleeved outside the rotary valve member 41.

[0151] Since the piercing member 322 is sleeved outside the rotary valve member 41, the rotary valve member 41 does not need to occupy much additional space, so that the overall structure of the detection instrument 10 can be made more compact. At the same time, in the case that the piercing member 322 is sleeved outside the rotary valve member 41, the rotary valve member 41 and the piercing member 322 do not interfere with each other, and in particular, can adapt to the characteristics shown in Figures 2-4 that the valve rod 203b is surrounded by the annular member 204h and the connecting rib 204d, so that the piercing member 322 and the rotary valve member 41 can act on the annular member 204h and the valve rod 203b, respectively.

[0152] In addition, referring to Figure 6 and Figure 7 In some embodiments, the end of the rotary valve member 41 close to the holding device 2 protrudes from the end of the piercing member 322 sleeved outside the rotary valve member 41 close to the holding device 2. This relative position corresponds to the characteristics shown in Figures 2-4 that the top end of the valve rod 203b is lower than the upper surface of the annular member 204h, so that the piercing member 322 and the rotary valve member 41 can act on the annular member 204h and the valve rod 203b, respectively.

[0153] When the end of the rotary valve member 41 close to the holding device 2 protrudes from the end of the piercing member 322 sleeved outside the rotary valve member 41 close to the holding device 2, the distance between the end of the rotary valve member 41 close to the holding device 2 and the end of the piercing member 322 close to the holding device 2 can be less than the distance between the top end of the rotor 203a and the plane on which the connecting rib 204d lies, i.e. the upper surface of the annular member 204h in Figures 2-4 , so that the rotary valve member 41 starts to combine with the rotor 203a only after the piercing member 322 breaks the connecting rib 204d. This is conducive to preventing the force between the rotary valve member 41 and the rotor 203a from affecting the smooth implementation of the piercing function, and makes the connection between the rotary valve member 41 and the rotor 203a occur before the second-stage pressing of the piercing mechanism 32 on the microfluidic chip 20, and after the piercing of the sealing film, so that the piercing step, the rotary valve step and the second-stage pressing step can be performed in sequence more conveniently.

[0154] In addition, referring to Figures 6-10In some embodiments, not only the valve element 41 of the valve device 4 is arranged on the piercing mechanism 32, but also the valve driving mechanism 43 of the valve device 4 is arranged on the piercing mechanism 32, so that the layout of the detection instrument 10 is more compact, and the valve driving mechanism 43 is more convenient to be drivingly connected with the valve element 41 to drive the valve element 41 to rotate.

[0155] Next, the communication nozzle 52 will be described.

[0156] In the related art, the communication nozzle 52 is not integrated on the piercing device 3, but is arranged independently of the piercing device 3, and the action of the communication nozzle 52 approaching or moving away from the holding device 2 to connect or separate from the communication port 204b of the microfluidic chip 20 is driven by a special driving mechanism, so that the structure is relatively complex, the cost is relatively high, and the volume is relatively large.

[0157] In the embodiments of the present application, the communication nozzle 52 is no longer arranged independently of the piercing device 3, but is integrated on the piercing mechanism 32, so that the structure of the detection instrument 10 is more compact, and since the communication nozzle 52 can move together with the piercing mechanism 32 under the driving of the piercing driving mechanism 33, it is not necessary to separately drive the communication nozzle 52 to move, nor is it necessary to separately provide the communication nozzle 52 with a driving mechanism, so that the structure is simple, the control is convenient, the volume is small, and the efficiency is high.

[0158] It can be seen that arranging the communication nozzle 52 on the piercing mechanism 32 can effectively improve the performance of the detection instrument 10.

[0159] In the case where the communication nozzle 52 is arranged on the piercing mechanism 32, referring to Figures 6-7 and Figures 11-12 In some embodiments, the pump assembly 5 includes a constraint element 54 and an elastic element 55, the constraint element 54 supports the communication nozzle 52, and the elastic element 55 is sleeved outside the communication nozzle 52 and abuts against the constraint element 54 to apply an elastic force to the communication nozzle 52 towards the communication port 204b. Based on this, the communication nozzle 52 has a certain activity space, which is beneficial to prevent damage to the communication nozzle 52 during connection with the communication port 204b due to misoperation or abnormal driving mechanism, etc.

[0160] Next, the detection instrument 10 shown in Figures 5-16 will be further introduced.

[0161] First of all, it needs to be pointed out that the orientation words appearing in the following text, such as "front, back, up, down, left, right", "transverse, vertical, perpendicular, horizontal", and "top, bottom" and the like, all indicate the orientation or positional relationship based on Figure 1 and Figure 5 shown in the orientation or positional relationship, wherein: the relative arrangement direction of the holding device 2 and the temperature control device 6 is the front-back direction, and Figure 1 andFigure 5 In the coordinate system X, which can also be referred to as the first direction X, the orientation in which the holding device 2 is located relative to the temperature control device 6 is front, and the orientation in which the temperature control device 6 is located relative to the holding device 2 is back; at the same time, the relative arrangement direction of the base 1 and the holding device 2 is the up-down direction, and in the up-down direction, Figure 1 In the coordinate system Z, which can also be referred to as the second direction Z, the orientation in which the base 1 is located relative to the holding device 2 is down, and the orientation in which the holding device 2 is located relative to the base 1 is up; and in the left-right direction perpendicular to the first direction X and the second direction Z, Figure 1 In the coordinate system Y, which can also be referred to as the third direction Y, the left-right direction is defined when facing the front. The orientation relationship thus defined is consistent with the orientation relationship when the detector 10 is normally placed.

[0162] The detector 10 of this embodiment is used to manipulate the microfluidic chip 20 shown in Figures 2-4 to realize automatic control of each step of nucleic acid detection based on the microfluidic chip 20, so as to improve the microfluidic detection efficiency and improve the application prospect of microfluidic technology.

[0163] As shown in Figures 5-16 In this embodiment, the detector 10 includes a base 1, a holding device 2, a piercing device 3, a pushing mechanism 35, a rotary valve device 4, a pump assembly 5, a temperature control device 6, an ultrasonic device 7, a magnetic attraction device 8, and a detection device 91.

[0164] The base 1 is used to provide a mounting basis for the holding device 2 and other modules to realize mounting and fixation of the other modules. The holding device 2, the piercing device 3, the pushing mechanism 35, the rotary valve device 4, the pump assembly 5, the temperature control device 6, the ultrasonic device 7, the magnetic attraction device 8, and the detection device 91 are all arranged on the base 1, and the communication device 5a of the pushing mechanism 35, the rotary valve device 4, and the pump assembly 5 are all integrated on the piercing device 3 to form a piercing assembly 101.

[0165] Since the specific structures of the base 1, the driving pump 51 of the pump assembly 5, the ultrasonic device 7, the magnetic attraction device 8, and the detection device 91 are not the focus of improvement of the present application, they will not be described here.

[0166] Next, the holding device 2, the piercing assembly 101, and the temperature control device 6 will be mainly introduced.

[0167] First, the holding device 2 is introduced.

[0168] The holding device 2 is used to hold and transport the microfluidic chip 20 to a target position for cooperation with other modules, so that the microfluidic chip 20 is manipulated by the piercing device 3, the valve rotating device 4 and the like, and the holding device 2 is also used to transport the microfluidic chip 20 away from the target position after detection, so that the microfluidic chip 20 is transported outside the detection instrument 10. In this embodiment, the target position is the position where the amplification cavity 205a of the microfluidic chip 20 is inserted between the two temperature control modules 61 of the temperature control device 6.

[0169] The process of transporting the microfluidic chip 20 into and out of the detection instrument 10 by the holding device 2 is respectively referred to as the warehousing process and the warehousing-out process.

[0170] In this embodiment, the holding device 2 is horizontally movable, specifically, the holding device 2 is movable forward and backward, so that the holding device 2 can not only hold the microfluidic chip 20, but also realize the transportation of the microfluidic chip 20, so that the microfluidic chip 20 reaches or leaves the target position. Moreover, the holding device 2 moves in the horizontal direction, but not in the vertical direction, which is beneficial to reducing the volume of the detection instrument 10, and facilitating cooperation with the piercing mechanism 32, the pushing mechanism 35, the valve rotating device 4 and the connecting nozzle 52 of the piercing assembly 101 which move in the vertical direction, to realize the required functions.

[0171] Next, the piercing assembly 101 is introduced.

[0172] Figures 6-12 The structure of the piercing assembly 101 in this embodiment is shown.

[0173] As shown in Figures 6-12 , in this embodiment, the piercing assembly 101 includes the piercing device 3, the pushing mechanism 35, the valve rotating device 4 and the connecting device 5a of the pump assembly 5, so that the piercing assembly 101 integrates the functions of piercing the sealing film, opening the temperature control device, controlling the valve angle and connecting the pump assembly with the microfluidic chip.

[0174] Here, the piercing device 3, the pushing mechanism 35, the valve rotating device 4 and the connecting device 5a are introduced respectively.

[0175] First, the piercing device 3 is introduced.

[0176] The piercing device 3 is used to realize the function of piercing the sealing film, and provides a mounting basis for the pushing mechanism 35, the valve rotating device 4 and the connecting device 5a, so that the pushing mechanism 35, the valve rotating device 4 and the connecting device 5a are integrated on the piercing device 3.

[0177] As shown in Figures 6-9 , in this embodiment, the piercing device 3 includes a piercing support 31, a piercing mechanism 32, a piercing driving mechanism 33 and a piercing detection device 34.

[0178] The piercing support 31 is arranged on the base 1 and supports the piercing mechanism 32, the piercing driving mechanism 33 and the piercing detection device 34. Specifically, as shown in the figure, in this embodiment, the piercing support 31 comprises a first vertical plate 311, a second vertical plate 312 and a horizontal plate 313. The first vertical plate 311 and the second vertical plate 312 are arranged in a spaced manner along the left-right direction, and the bottom ends of the two are fixed on the first side plate 12 and the second side plate 13 of the base 1 respectively, so as to realize the mounting and fixing of the piercing device 3 on the base 1. The horizontal plate 313 is connected to the top ends of the first vertical plate 311 and the second vertical plate 312, and is used to support the piercing motor 331 of the piercing driving mechanism 33. Figures 6-9

[0179] The piercing mechanism 32 is arranged on the piercing support 31 in a movable manner along the up-down direction, and comprises a support plate 321 and a piercing piece 322. The left and right ends of the support plate 321 are slidably connected to the first vertical plate 311 and the second vertical plate 312 respectively, and the piercing piece 322 is arranged on the support plate 321, so that when the support plate 321 moves along the up-down direction, the piercing piece 322 can move up and down together. Specifically, as shown in the figure, the left and right ends of the support plate 321 are connected to piercing sliding blocks 323, and the piercing sliding blocks 323 are slidably arranged on piercing guide rails 324, and the piercing guide rails 324 are fixed on the vertical plate (i.e. the first vertical plate 311 or the second vertical plate 312), so that the support plate 321 is slidably connected to the vertical plate through the guide rail sliding block mechanism. The piercing piece 322 is fixed on the lower surface of the support plate 321 and has a hollow cylindrical shape, so that the piercing piece 322 is pressed on the top cover 204 of the microfluidic chip 20 to press and break the connecting ribs 204d on the top cover 204 for connecting the annular piece 204h and the cover body 204g. Once all the connecting ribs 204d are broken, the annular piece 204h can be separated from the cover body 204g, and the piercing needle 204c falls down to pierce the sealing film at the top end of the storage cavity 201a of the microfluidic chip 20, so as to realize the piercing function. Figure 13

[0180] It can be seen that based on the movable support plate 321 and the piercing piece 322 arranged on the support plate 321, the piercing mechanism 32 has the sealing film piercing function, which facilitates the flow of fluid in the microfluidic chip 20.

[0181] In addition to being able to realize the sealing film piercing function, the piercing mechanism 32 can also realize the resealing function of the storage cavity 201a.

[0182] ​​Before the piercing needle 204c pierces the sealing film to the end of the extraction step, the piercing needle 204c is kept in the position of piercing the sealing film and connecting the storage cavity 201a with the atmosphere under the abutting action of the stopper 204e and the body 201. In this process, the piercing member 322 does not break the connecting ring 204h and the rib 204f of the stopper 204e, but waits until the fluid in the reaction cavity 202a has been transferred into the amplification member 205a after the end of the extraction. Then, the piercing member 322 is driven by the support plate 321 to continue to press the connecting ring 204h, breaks the connecting ring 204h and the rib 204f of the stopper 204e completely, drives the piercing needle 204c to move further towards the holding device 2 by the connecting ring 204h, until the vent hole of the piercing needle 204c is blocked by the cover sheet 206 of the microfluidic chip 20, and the storage cavity 201a is resealed to avoid accidental leakage of the liquid in the storage cavity 201a and affect the normal operation of the detection instrument 10.

[0183] It can be seen that the piercing mechanism 32 can realize two-stage pressing of the microfluidic chip 20, respectively meeting the piercing requirement of the sealing film before the extraction starts and the resealing requirement of the storage cavity 201a after the extraction ends.

[0184] The piercing driving mechanism 33 is arranged on the piercing support 31 and is used to drive the piercing mechanism 32 to move relative to the piercing support 31. As shown in Figures 8-9 the embodiment, the piercing driving mechanism 33 includes a piercing motor 331, a piercing gear 332 and a piercing rack 333. The piercing motor 331 is connected with the horizontal plate 313 and the second vertical plate 312 to realize installation on the piercing support 31. The piercing motor 331 is drivingly connected with the piercing gear 332. The piercing gear 332 is engaged with the piercing rack 333. The piercing rack 333 extends along the up-down direction and is connected with the support plate 321 of the piercing mechanism 32. In this way, when the piercing motor 331 rotates, the support plate 321 can be driven to move up and down by the piercing gear 332 and the piercing rack 333, and then drive the piercing member 322 to move up and down, so as to realize the up-down movement of the piercing mechanism 32 relative to the piercing support 31, and make the piercing mechanism 32 away from or close to the holding device 2, and press the microfluidic chip 20 in two stages during the process of closing to the holding device 2.

[0185] The piercing detection device 34 is used to detect the position of the piercing mechanism 32. As shown in Figures 8-9As shown, in this embodiment, the piercing detection device 34 comprises a first piercing detection member 341, a second piercing detection member 342 and a piercing trigger member 343. The first piercing detection member 341 and the second piercing detection member 342 are both arranged on the first vertical plate 311, and are arranged in sequence along the direction from top to bottom (i.e. the direction in which the piercing mechanism 32 approaches the holding device 2), that is, the first piercing detection member 341 is located above the second piercing detection member 342. The piercing trigger member 343 is arranged on the piercing mechanism 32, specifically, the piercing trigger member 343 is arranged on the support plate 321 of the piercing mechanism 32, so that the piercing trigger member 343 can move together with the piercing mechanism 32. Exemplarily, the first piercing detection member 341 and the second piercing detection member 342 are both photoelectric switches, and correspondingly, the piercing trigger member 343 is a baffle.

[0186] In operation, the first piercing detection member 341 cooperates with the piercing trigger member 343 to detect whether the piercing mechanism 32 is located at the initial position. The second piercing detection member 342 cooperates with the piercing trigger member 343 to constitute an opening detection mechanism 345, which detects whether the piercing mechanism 32 drives the poking mechanism 35 to reach the opening position (i.e. the position at which the poking mechanism 35 separates the two temperature control modules 61 of the temperature control device 6), so as to determine whether the two temperature control modules 61 have been opened by the poking mechanism 35.

[0187] As can be seen, under the cooperation of the first piercing detection member 341, the second piercing detection member 342 and the piercing trigger member 343, the piercing detection device 34 can detect whether the piercing mechanism 32 reaches the initial position and the opening position.

[0188] Among them, the piercing detection device 34 is used to detect whether the piercing mechanism 32 is at the initial position, on the one hand, it is convenient to judge whether the piercing mechanism 32 is accurately reset after detection, so as to avoid affecting the smooth progress of next operation due to the inaccurate reset of the piercing mechanism 32, on the other hand, it is also convenient to take the initial position as a reference, and combine the setting of the working parameters of the piercing motor 331, to accurately control the piercing function and the connection between the rotor 203a (specifically, the valve rod 203b) of the microfluidic chip 20 and the valve device 4.

[0189] Specifically, before the detection process starts, the piercing mechanism 32 is determined whether it is in the initial position by the piercing detection device 34, and the distance between the piercing member 322 and the microfluidic chip 20 is determined, and the number of rotations of the piercing motor 331 required from the initial position to the position of pressing the connecting rib 204d (i.e. the position of the piercing mechanism 32) is obtained, and then after the detection flow is started, the piercing motor 331 is controlled to rotate the number of rotations set in advance, so as to achieve the piercing of the sealing film; then, according to the initial position and the distance between the valve rotating device 4 and the microfluidic chip 20, the number of rotations of the piercing motor 331 required to connect the valve rotating device 41 (specifically the valve rotating shaft 411) and the rotor 203a of the microfluidic chip 20 is obtained, and after the sealing film is pierced, the piercing motor 331 is controlled to continue to work to reach the corresponding number of rotations, so as to complete the connection of the valve rotating device 41 and the rotor 203a, so that the valve rotating device 41 rotates the rotor 203a to realize the valve position switching of the microfluidic chip 20.

[0190] It can be seen that based on the piercing detection device 34 and the piercing motor 331, the embodiment can accurately control the piercing and the connection of the valve rotating device 4 and the microfluidic chip 20 according to the position information provided by the piercing detection device 34 and the parameter setting of the piercing motor 331. Since no additional detection components are required to detect the position of pressing the connecting rib 204d and the connection position of the valve rotating device 4 and the microfluidic chip 20, the structure is relatively simple and the cost is relatively low.

[0191] The piercing detection device 34 is used to detect whether the piercing mechanism 32 reaches the open position, which is beneficial to determine whether the microfluidic chip 20 can be controlled by the holding device 2 to reach the target position or leave the target position.

[0192] In the initial stage of the entire detection process, the microfluidic chip 20 is controlled by the holding device 2 to reach the target position after the piercing mechanism 32 moves downward to the open position and the two temperature control modules 61 are separated by the separating mechanism 35, that is, the piercing mechanism 32 drives the separating mechanism 35 to reach the separated position before the amplification cavity 205a reaches the target position, in other words, after the piercing detection device 34 detects that the piercing mechanism 32 has reached the open position, the microfluidic chip 20 is controlled to reach the target position with the holding device 2, so that the amplification cavity 205a of the microfluidic chip 20 is inserted between the two temperature control modules 61. In this way, the amplification cavity 205a is inserted after the two temperature control modules 61 are separated from each other, which can reduce the wear during the insertion process.

[0193] In addition, in this embodiment, after the whole detection process is completed, the piercing mechanism 32 is first controlled to move upward to the open position, the dislodging mechanism 35 is controlled to separate the two temperature control modules 61 again, and then the holding device 2 is controlled to drive the microfluidic chip 20 to start moving away from the target position. In other words, the piercing mechanism 32 drives the dislodging mechanism 35 to reach the open position before the amplification cavity 205a starts moving away from the target position. In other words, after the piercing detection device 34 detects that the piercing mechanism 32 has reached the open position, the holding device 2 is controlled to start driving the microfluidic chip 20 to move outward and move out from between the two temperature control modules 61. In this way, the amplification cavity 205a moves outward only when the two temperature control modules 61 are separated from each other, which can reduce the wear during the moving-out process and prevent the leakage of residual liquid in the amplification cavity 205a, which may cause pollution.

[0194] As can be seen, by arranging the dislodging mechanism 35 on the piercing mechanism 32 and providing the piercing detection device 34 to detect whether the piercing mechanism 32 reaches the open position, the dislodging mechanism 35 can be controlled to open the two temperature control modules 61 before the amplification cavity 205a is inserted and starts to move out, so as to reduce the wear of the microfluidic chip 20 during the insertion and moving-out process.

[0195] Next, the dislodging mechanism 35 will be introduced.

[0196] The dislodging mechanism 35 is used to separate the two temperature control modules 61 of the temperature control device 6, so as to insert and pull out the amplification cavity 205a of the microfluidic chip 20.

[0197] As shown in Figure 8 , in this embodiment, the dislodging mechanism 35 is arranged on the piercing mechanism 32 and includes a rotating bearing 352 and a connecting shaft 353. The connecting shaft 353 is connected to the support plate 321 of the piercing mechanism 32. The rotating bearing 352 serves as a dislodging piece 351, which is rotatably sleeved on the connecting shaft 353 and located at the rear side of the support plate 321, so as to act on the temperature control device 6 located at the rear side of the support plate 321. Specifically, in this embodiment, the rotating bearing 352 is a deep groove ball bearing.

[0198] Based on the above arrangement, the rotating bearing 352 can move together with the piercing mechanism 32, and when it moves to the open position, as shown in Figure 15 , the rotating bearing 352 separates the two temperature control modules 61; and when it leaves the open position, as shown in Figure 16 , the rotating bearing 352 stops acting on the temperature control device 6, and the two temperature control modules 61 are recombined with each other or with the amplification cavity 205a inserted therebetween, so as to reset the two temperature control modules 61 or control the temperature of the amplification cavity 205a inserted therebetween.

[0199] As mentioned above, since the poking mechanism 35 is arranged on the piercing mechanism 32, the structure is more compact, and it is not necessary to separately control the movement of the poking mechanism 35, and it is not necessary to additionally arrange a driving mechanism to drive the movement of the poking mechanism 35, thus the structure is simpler, the control is more convenient, the cost is lower, and the efficiency is higher.

[0200] Next, the rotary valve device 4 is introduced.

[0201] The rotary valve device 4 is used to rotate the switching valve 203 of the microfluidic chip 20, so as to sequentially control the switching communication of the reaction cavity 202a with each storage cavity 201a and the amplification cavity 205a, to provide conditions for realizing the liquid flow control of the microfluidic chip 20.

[0202] As shown in the drawings, Figures 6-10 in this embodiment, the rotary valve device 4 includes a rotary valve piece 41, a rotary valve support 42, a rotary valve driving mechanism 43, and an angle detection device 44.

[0203] The rotary valve piece 41 is used to be connected with the rotor 203a of the switching valve 203 of the microfluidic chip 20, so as to drive the rotation of the rotor 203a and realize the valve position switching.

[0204] It can be known from Figure 7 , Figure 8 and Figure 10 that in this embodiment, the rotary valve piece 41 is a rotary valve shaft 411, which is rotatably penetrated through the support plate 321 of the piercing mechanism 32, and the upper end is constrained by the rotary valve support 42 fixed on the support plate 321, and the lower end is penetrated through the piercing piece 322 of the piercing mechanism 32, so that the rotary valve shaft 411 is rotatably arranged on the piercing mechanism 32. Bearings (not shown in the drawings) are arranged between the rotary valve shaft 411 and the rotary valve support 42 and between the rotary valve shaft 411 and the support plate 321, so as to facilitate the rotation of the rotary valve shaft 411.

[0205] In this embodiment, the rotary valve shaft 411 has an inner hole (not shown in the drawings), so that the rotary valve shaft 411 can be sleeved on the valve rod 203b of the microfluidic chip 20. Moreover, the inner hole of the rotary valve shaft 411 is hexagonal, which is matched with the outer surface shape of the valve rod 203b, so that after being sleeved on the valve rod 203b, the rotary valve shaft 411 and the valve rod 203b will not relatively rotate, so that the rotation of the rotary valve shaft 411 can reliably drive the rotation of the valve rod 203b, realize the valve position switching, and make the liquid in the microfluidic chip 20 flow in sequence.

[0206] Moreover, in this embodiment, along the direction in which the rotary valve shaft 411 approaches the holding device 2, the cross-sectional dimensions of one of the inner hole of the rotary valve shaft 411 and the valve stem 203b remain unchanged, while the other changes, so that the connection tightness between the rotary valve shaft 411 and the valve stem 203b increases along the direction in which the rotary valve shaft 411 approaches the holding device 2. For example, the cross-sectional dimension of the inner hole of the rotary valve shaft 411 remains unchanged in the direction in which the rotary valve shaft 411 approaches the holding device 2, while the cross-sectional dimension of the valve stem 203b increases along the direction in which the rotary valve shaft 411 approaches the holding device 2 (i.e., from top to bottom); or, the cross-sectional dimension of the valve stem 203b remains unchanged in the direction in which the rotary valve shaft 411 approaches the holding device 2, while the cross-sectional dimension of the inner hole of the rotary valve shaft 411 decreases along the direction in which the rotary valve shaft 411 approaches the holding device 2 (i.e., from top to bottom). In this way, when the rotary valve shaft 411 is initially sleeved on the valve stem 203b, the inner wall of the rotary valve shaft 411 is not tightly fitted with the outer surface of the valve stem 203b, but there is a gap. Afterwards, as the sleeve depth increases, the connection tightness between the rotary valve shaft 411 and the valve stem 203b gradually increases. When the rotary valve shaft 411 is sleeved on the valve stem 203b, the connection tightness between the rotary valve shaft 411 and the valve stem 203b is the largest, and the two are relatively fixed. That is to say, as the piercing mechanism 32 drives the rotary valve shaft 411 close to the microfluidic chip 20, the rotary valve shaft 411 and the valve stem 203b are relatively fixed. The tightness of the connection between them gradually increases. In this way, on the one hand, it is convenient to control the rotary valve component 41 to be connected to the rotor 203a only after the puncture function is realized, so as to prevent the rotary valve device 4 from affecting the smooth realization of the puncture function. On the other hand, it is also beneficial to correct the microfluidic chip 20 to a certain extent when the microfluidic chip 20 is deflected. Even in extremely special circumstances, the microfluidic chip 20 is deflected before the rotary valve component 41 is connected to the microfluidic chip 20, the cooperation between the rotary valve component 41 and the valve stem 203b can be used to gradually straighten the microfluidic chip 20.

[0207] In addition, if Figure 7 and Figure 8 As shown, in this embodiment, the lower end of the rotary valve shaft 411 is lower than the lower end of the piercing member 322. Figures 2-4 As shown, the upper surface of the valve stem 203b of the microfluidic chip 20 is lower than the upper surface of the top cover 204 (i.e., the plane where the connecting rib 204d is located), which is adapted to the characteristic that the valve shaft 411 is conveniently sleeved on the valve stem 203b to achieve connection with the valve stem 203b.

[0208] And, in this embodiment, the lower end of the rotary valve shaft 411 is lower than the lower end of the piercing member 322, and the height of the upper surface of the valve stem 203b lower than the upper surface of the top cover 204, which makes the rotary valve shaft 411 not yet sleeved on the valve stem 203b when the piercing member 322 is lowered to the pressure breaking position and the upper surface of the top cover 204 is pressed to break the connecting rib 204d. On the one hand, this facilitates the rotary valve shaft 411 to be sleeved on the valve stem 203b after the piercing function is realized, so as to complete the connection between the rotary valve device 4 and the rotor 203a, thereby preventing the rotary valve member 41 from being connected with the rotor 203a before the piercing function is realized, and further preventing the piercing function from being affected by the force applied to the microfluidic chip 20 due to the connection between the rotary valve member 41 and the microfluidic chip 20, etc. On the other hand, the height of the lower end of the rotary valve shaft 411 exceeding the lower end of the piercing member 322 is appropriate, and will not be too much or too little, which facilitates the rotary valve shaft 411 to be sleeved on the valve stem 203b, and will not affect the realization of the secondary pressure function of the piercing mechanism 32 on the microfluidic chip 20 due to the excessive length of the rotary valve shaft 411.

[0209] The rotary valve driving mechanism 43 is used to drive the rotary valve member 41 to rotate, so as to drive the rotor 203a to rotate and realize the valve position switching.

[0210] As shown in Figure 10 the embodiment, the rotary valve driving mechanism 43 includes a rotary valve motor 431 and a rotary valve transmission mechanism 432. The rotary valve motor 431 is drivingly connected with the rotary valve member 41 (i.e. the rotary valve shaft 411) through the rotary valve transmission mechanism 432, so as to drive the rotary valve member 41 to rotate. Specifically, in this embodiment, the rotary valve motor 431 and the rotary valve transmission mechanism 432 are both arranged on the support plate 321 of the piercing mechanism 32. Moreover, the rotary valve transmission mechanism 432 is a belt transmission mechanism 433. The belt transmission mechanism 433 includes a belt 436 and two pulleys 434. One of the two pulleys 434 is drivingly connected with the rotary valve motor 431, and the other is drivingly connected with the rotary valve member 41. The belt 436 is connected between the two pulleys 434, so that the belt transmission mechanism 433 can realize the driving connection between the rotary valve motor 431 and the rotary valve member 41, thereby enabling the rotary valve member 41 to be driven to rotate by the belt transmission mechanism 433 when the rotary valve motor 431 rotates.

[0211] It can be understood that, in addition to the belt transmission mechanism 433, the rotary valve transmission mechanism 432 can also be a chain transmission mechanism or other transmission mechanisms.

[0212] Continuing to refer to 10, in this embodiment, the rotary valve driving mechanism 43 further comprises a tensioning member 437, which is arranged on the piercing mechanism 32 and tensions the belt 436 of the belt driving mechanism 433 to prevent the belt 436 from loosening and affecting the smooth implementation of the rotary valve function. Specifically, in this embodiment, the tensioning member 437 comprises a tensioning pin, which is arranged on the support plate 321. Of course, when the rotary valve driving mechanism 432 is a chain driving mechanism, the tensioning member 437 can also be arranged and used to tension the chain of the chain driving mechanism.

[0213] The angle detecting device 44 is used to detect the rotation angle of the rotary valve member 41 to determine the rotary valve angle and achieve accurate feedback of the rotary valve angle, forming a closed loop control, so as to more accurately control the fluid to flow in the desired order.

[0214] As shown in Figure 10 In this embodiment, the angle detecting device 44 comprises an angle detecting member 441, a mounting seat 443 and a magnet seat 446. The angle detecting member 441 comprises a Hall magnet (not shown in the figure) and a Hall sensor 442. The Hall magnet is embedded on the magnet seat 446, which is connected with the rotary valve member 41, so that the Hall magnet can rotate with the rotary valve member 41. The Hall sensor 442 is arranged on the mounting seat 443, which is fixed on the rotary valve support 42, so that the Hall sensor 442 does not rotate with the rotary valve member 41. The Hall sensor 442 is vertically opposite to the Hall magnet. In this way, when the rotary valve member 41 rotates, the Hall sensor 442 can detect the rotation angle of the Hall magnet to detect the rotation angle of the rotary valve member 41, so as to determine the rotary valve angle.

[0215] Since the rotary valve member 41, the rotary valve support 42, the rotary valve driving mechanism 43 and the angle detecting device 44 are all arranged on the piercing device 3, the rotary valve device 4 is arranged on the piercing device 3 as a whole, which is more compact in structure and is conducive to reducing the overall volume of the detector 10 and saving the floor area of the detector 10.

[0216] Moreover, since the rotary valve member 41 is arranged on the piercing mechanism 32 and moves with the piercing mechanism 32 under the driving of the piercing driving mechanism 33, it is not necessary to separately control the movement of the rotary valve member 41 or to separately provide a driving mechanism for driving the movement of the rotary valve member 41, so that the structure is relatively simple, the control is relatively convenient, the cost is relatively low and the efficiency is relatively high.

[0217] The microfluidic chip 20 is fixed, the corresponding valve angle of each storage cavity 201a and the reaction cavity 202a in communication and the reaction cavity 202a and the amplification cavity 205a in communication is fixed, therefore, after the sealing film is pierced by the piercing mechanism 32, the rotation angle of the valve piece 41 is controlled, and then the liquid flow power is provided by the pump assembly 5, so that the different steps of nucleic acid extraction and amplification can be controlled.

[0218] Next, the communication device 5a is introduced.

[0219] The communication device 5a is a component of the pump assembly 5, which is used to communicate the driving pump 51 of the pump assembly 5 with the communication port 204b of the microfluidic chip 20, so that the driving pump 51 can drive the fluid in the microfluidic chip 20 to flow in the desired order by applying negative pressure or positive pressure to the microfluidic chip 20.

[0220] As shown in Figures 6-7 and Figures 11-12 In this embodiment, the communication device 5a includes a communication nozzle 52, a clamp 53, a constraint piece 54, an elastic piece 55 and a guide bearing 56.

[0221] The communication nozzle 52 is used to communicate the driving pump 51 and the communication port 204b, which is arranged on the support plate 321 of the piercing mechanism 32 and is constrained by the clamp 53 to realize the arrangement of the communication nozzle 52 on the support plate 321. Specifically, in this embodiment, the communication nozzle 52 is in the form of a rod, the upper end and the lower end of which pass through the clamp 53, and the upper end extends above the support plate 321 for connecting with the driving pump 51, while the lower end extends below the support plate 321 for connecting with the communication port 20b. The clamp 53 and the support plate 321 are connected together by bolts or the like to realize the installation of the communication nozzle 52 on the support plate 321. More specifically, as shown in Figure 12 In this embodiment, the communication nozzle 52 is arranged on the front side of the support plate 321, at this time, the relative position relationship between the communication nozzle 52 and the support plate 321 is consistent with the relative position relationship between the holding device 2 and the support plate 321, which is more convenient for the communication nozzle 52 to connect with the communication port 204b of the microfluidic chip 20 installed on the holding device 2.

[0222] Combined with Figure 10 and Figure 11It can be known that in the embodiment, the first groove portion 326 is arranged on the support plate 321, the second groove portion 532 is arranged on the clamp 53, the first groove portion 326 and the second groove portion 532 are in butt joint, the closed groove is formed, and the communication nozzle 52 is embedded in the closed groove. Based on this, on one hand, the installation effect of the communication nozzle 52 is more stable and is not easy to fall off; on the other hand, the positions of the communication nozzle 52 and the communication port 204b of the microfluidic chip 20 in the target position in the front-back direction are also more consistent, and it is more convenient for the communication nozzle 52 to be inserted into the communication port 204b; on the other hand, the communication nozzle 52 does not completely protrude from the support plate 321 in the horizontal direction, and the occupied space is smaller. Of course, as a variant, only the first groove portion 326 can be arranged on the support plate 321, or only the second groove portion 532 can be arranged on the clamp 53.

[0223] In addition, as shown in the embodiment, the avoiding port 325 is arranged on the piercing member 322, and the avoiding port 325 faces the communication nozzle 52 to avoid the communication nozzle 52. In this way, the interference between the communication nozzle 52 and the piercing member 322 can be prevented while the structure is relatively compact. Figure 9

[0224] Since the communication nozzle 52 is arranged on the piercing mechanism 32, the structure is more compact, and the communication nozzle 52 can be close to or away from the holding device 2 together with the piercing mechanism 32 under the driving of the piercing driving mechanism 33 to connect or separate from the microfluidic chip 20, without the need to separately control the movement of the communication nozzle 52 and without the need to separately provide the communication nozzle 52 with a driving mechanism for driving the movement of the communication nozzle 52. Therefore, the structure is relatively simple, the control is relatively convenient, the cost is relatively low, and the efficiency is relatively high.

[0225] The constraint member 54 and the elastic member 55 cooperate together to adjust the up-down position of the communication nozzle 52. Referring to Figures 11-12 , in combination with Figure 6 and Figure 7 ​In this embodiment, the constraint member 54 is in the shape of a plate, which is located below the support plate 321 and fixedly connected with the clamp 53. The communication nozzle 52 penetrates through the constraint member 54. The elastic member 55 (for example, a spring 551) is sleeved on the communication nozzle 52 and abuts between the constraint member 54 and the limiting step 521 of the communication nozzle 52, so as to exert an elastic force downward on the communication nozzle 52. The advantage of this arrangement is that, on one hand, after the communication nozzle 52 is connected with the communication port 204b, the elastic member 55 can exert a certain pressure on the communication nozzle 52, so as to increase the connection firmness of the communication nozzle 52 and the communication port 204b and prevent the communication nozzle 52 from being accidentally disconnected; on the other hand, the elastic member 55 allows the communication nozzle 52 to have a certain moving space, so that the communication nozzle 52 can move up and down within a certain range when subjected to an external force, so as to adjust the position of the communication nozzle 52. If the communication nozzle 52 and the communication port 204b are not vertically aligned during the connection process due to an unexpected situation, the communication nozzle 52 cannot be smoothly inserted into the communication port 204b. In this case, the communication nozzle 52 can move up and down within a certain range under the action of the elastic member 55, so as to prevent the communication nozzle 52 from being damaged due to hard contact with the microfluidic chip 20. When an abnormal situation occurs, the protection of the communication nozzle 52 can be realized by damaging the elastic member 55.

[0226] The guide bearing 56 is sleeved on the communication nozzle 52 and used to guide the up-and-down movement of the communication nozzle 52. Specifically, in this embodiment, the guide bearing 56 is a linear bearing, which is embedded in the second groove portion 532 and the corresponding groove of the valve holder 42. The guide bearing 56, together with the elastic member 55 and the like, can guide the communication nozzle 52 to move more accurately in the up-and-down direction relative to the piercing mechanism 32, so as to prevent the communication nozzle 52 from being deflected during the up-and-down movement relative to the piercing mechanism 32 and affect the smooth connection of the communication nozzle 52 and the communication port 204b.

[0227] As described above, by arranging the prying mechanism 35, the valve device 4 and the communication nozzle 52 on the piercing mechanism 32, the prying mechanism 35, the valve device 4 and the communication nozzle 52 can move together with the piercing mechanism 32, which can effectively simplify the structure and control process of the detection instrument 10, reduce the size of the detection instrument 10 and improve the efficiency of the detection instrument 10.

[0228] Next, the temperature control device 6 will be described.

[0229] The temperature control device 6 is fixed to the base 1 and located at the rear side of the holding device 2, and is used to control the temperature of the amplification cavity 205a of the microfluidic chip 20 on the holding device 2, so as to provide a suitable and stable temperature condition for the amplification reaction and enable the amplification reaction to be carried out smoothly.

[0230] Figures 13-14 The structure of the temperature control device 6 in this embodiment is shown.

[0231] As Figure 13 andFigure 14 As shown, in this embodiment, the temperature control device 6 includes a support plate 63, a pull plate 64, and two temperature control units 66. The two temperature control units 66 have the same structure and each includes a temperature control module 61, a sliding assembly 612, a support plate 65, and an opening mechanism 62, so that the temperature control device 6 includes two temperature control modules 61, two sliding assemblies 612, two support plates 65, and two opening mechanisms 62.

[0232] The supporting plate 63 is fixed on the first side plate 12 and the second side plate 13 of the base 1 to support the two temperature control units 66 .

[0233] The pull plate 64 is connected to two support plates 65 fixed on the supporting plate 63 to prevent the two temperature control modules 61 from being unable to fit completely together after the temperature control device 6 has been working for a long time.

[0234] The temperature control module 61 is used to control the temperature of the amplification chamber 205 a. The two temperature control modules 61 are disposed on the support plate 63 so as to be relatively movably opposed to each other, and each module includes a heat exchange device 611 and a heat dissipation device 613.

[0235] The heat exchange device 611 is used to perform heat exchange with the amplification chamber 205a, heating and cooling the amplification chamber 205a, so as to control the temperature of the amplification chamber 205a to cycle between the first temperature and the second temperature during the nucleic acid amplification process to meet the nucleic acid amplification requirements.

[0236] like Figure 13 and Figure 14 As shown, in this embodiment, the heat exchange device 611 includes a semiconductor refrigeration plate 611a, a heat transfer plate 611b, a first positioning member 611c, and a second positioning member 611d. The semiconductor refrigeration plate 611a and the heat transfer plate 611b are arranged sequentially along a direction approaching another heat exchange device 611 and are respectively embedded in the first positioning member 611c and the second positioning member 611d. These positioning members 611c and 611d secure the semiconductor refrigeration plate 611a and the heat transfer plate 611b to their respective positions. The first positioning member 611c and the second positioning member 611d are fixedly connected, allowing the semiconductor refrigeration plate 611a and the heat transfer plate 611b to move together. The semiconductor refrigeration plate 611a utilizes the Peltier effect to generate heat and cool, achieving both heating and cooling. The heat transfer plate 611b is configured to contact the amplification chamber 205a and is bonded to the semiconductor refrigeration plate 611a via high-temperature silicone rubber to facilitate heat transfer between the semiconductor refrigeration plate 611a and the amplification chamber 205a.

[0237] The heat sink 613 is used to achieve heat transfer between the heat exchange device 611 and the environment to improve the temperature control efficiency. Figure 13 and Figure 14As shown, in this embodiment, the heat dissipation device 613 is arranged on the side of the heat exchange device 611 away from the other temperature control module 61, and includes a heat dissipation block 613a, a heat dissipation fan 613b, a heat dissipation block fixing member 613c, and a connecting block 613d. The heat dissipation fan 613b is in contact with the heat exchange device 611 through the heat dissipation block 613a. The heat dissipation block 613a is mounted on the heat dissipation block fixing member 613c, and is connected with the sliding assembly 612 through the connecting block 613d, so as to realize the movable arrangement of the heat dissipation device 613 and the heat exchange device 611 on the supporting plate 63.

[0238] The sliding assembly 612 connects the corresponding support plate 65 and the temperature control module 61. As shown, Figure 13 and Figure 14 As shown, the two support plates 65 are fixed on the supporting plate 63, and are located on the outer side of the corresponding temperature control module 61 respectively. The support plate 65 and the temperature control module 61 are connected through the sliding assembly 612, so that the two temperature control modules 61 can move away from each other when an external force is applied, and can move close to each other after the external force disappears. The sliding assembly 612 includes a temperature control elastic member 612a, a support shaft 612b, and a sliding guide mechanism 612c. The support shaft 612b connects the support plate 65 and the connecting block 613d. The temperature control elastic member 612a (for example, a spring) is sleeved on the support shaft 612b, and abuts against the support plate 65 and the connecting block 613d, so as to exert an elastic force on the temperature control module 61 to make the two temperature control modules 61 close to each other. The sliding guide mechanism 612c (for example, a linear guide rail slider) is arranged on the supporting plate 63 and connected with the connecting block 613d, so as to guide the relative movement of the two temperature control modules 61. In order to improve the stability of the two temperature control modules 61, in this embodiment, the sliding assembly 612 includes two temperature control elastic members 612a and two support shafts 612b. The two support shafts 612b of the same sliding assembly 612 are connected between the temperature control module 61 and the support plate 65 in front and side by side, and one temperature control elastic member 612a is sleeved on each support shaft 612b.

[0239] Initially, under the action of the temperature control elastic member 612a, the two temperature control modules 61 are attached together, specifically, the two heat transfer sheets 611b are attached together; when the two temperature control modules 61 are subjected to an outward pushing force, the two temperature control modules 61 move away from each other and are separated from each other, in this process, the two temperature control elastic members 612a are compressed and store elastic force; and when the outward pushing force disappears, the two temperature control elastic members 612a release the elastic force, so that the two temperature control modules 61 move close to each other, at this time, if there is no object (for example, the amplification member 205) between the two heat transfer sheets 611b, the two temperature control modules 61 are reattached together, and if the amplification member 205 has been inserted between the two heat transfer sheets 611b, the two temperature control modules 61 are attached to the amplification cavity 205a to heat or cool the amplification cavity 205a.

[0240] Since the temperature control device 6 does not move in the front-rear direction, the insertion and extraction of the amplification member 205 between the two temperature control modules 61 are realized by the front-rear movement of the holding device 2.

[0241] Since the holding device 2 needs to move originally to realize the process of putting the microfluidic chip into the warehouse and taking it out of the warehouse, the temperature control device 6 is arranged not to move in the direction of entering and leaving the warehouse, but directly relies on the movement of the holding device 2 in the direction of entering and leaving the warehouse to realize the insertion and extraction of the microfluidic chip 20 between the two temperature control modules 61, which is simple and convenient, and can save the driving mechanism for driving the temperature control device 6 to move in the direction of entering and leaving the warehouse.

[0242] The opening mechanism 62 is used in cooperation with the aforementioned push-open mechanism 35 to realize the pre-separation of the two temperature control modules 61 before the insertion and extraction of the amplification member 205, so as to reduce the difficulty of inserting and extracting the amplification member 205, reduce the wear of the amplification member 205 in the process of insertion and extraction, and prevent liquid leakage.

[0243] As shown in Figure 13 and Figure 14 , in this embodiment, the two opening mechanisms 62 are connected with the two temperature control modules 61 one by one. And the two opening mechanisms 62 are opposite to each other and have the same structure, both including an opening member 621, a reset elastic member 623 and a shaft body 624.

[0244] The opening member 621 is used to contact the push-open mechanism 35 and drive the connected temperature control module 61 away from the other temperature control module 61 under the action of the push-open mechanism 35, so as to realize the separation of the two temperature control modules 61. As shown in Figure 13 and Figure 14 , in this embodiment, the opening member 621 is installed on the heat exchange device 611 of the temperature control module 61, and is fixed on the heat dissipation block fixing piece 613c of the heat exchange device 611, so that when the opening member 621 is pushed outward, it can drive the temperature control module 61 to move outward and realize the separation of the two temperature control modules 61. Wherein, the surface of the opening member 621 facing the other opening member 621 is provided with a convex part 622, which is arc-shaped and located between the upper and lower ends of the opening member 621. The convex part 622 protrudes from the part of the opening member 621 located on both sides of the convex part 622. In this way, the opening member 621 contacts the push-open mechanism 35 through the convex part 622, so that the push-open mechanism 35 can apply a pushing force to the opening member 621, so that the two opening members 621 drive the two temperature control modules 61 away from each other, and separate the two temperature control modules 61. When the push-open mechanism 35 has not reached the convex part 622 or has passed the convex part 622, the push-open mechanism 35 no longer applies a pushing force to the opening member 621, so that the two opening members 621 can be reset under the action of the reset elastic member 623, and drive the two temperature control modules 61 to approach each other, and re-adhere to each other, or adhere to the amplification cavity 205a.

[0245] The return spring 623 is used to apply an elastic force to the opening members 621, forcing the two opening members 621 toward each other. It is mounted on a shaft 624. The ends of the shaft 624 are connected to the support plate 65 and the opening members 621, respectively. A step is provided on the shaft 624 to block the return spring 623, allowing the return spring 623 to abut between the step of the shaft 624 and the opening members 621, exerting an elastic force on the opening members 621, forcing the two opening members 621 toward each other. As an example, the return spring 623 is a spring 551.

[0246] When the spreading mechanism 35 applies a thrust to the two opening members 621, the two reset elastic members 623 are compressed more as the two opening members 621 move away from each other, storing elastic energy. Afterwards, when the spreading mechanism 35 continues to move downward and the thrust disappears, the two reset elastic members 623 release the stored elastic energy, pushing the two opening members 621 closer to each other.

[0247] Because the opening member 621 has an arcuate surface for contact with the dislodging mechanism 35, it can better cooperate with the rotating bearing 352 of the dislodging mechanism 35 to achieve the corresponding opening function. In addition, the arcuate surfaces on both sides of the most protruding portion of the protrusion 622 of the opening member 621 can also guide the rotating bearing 352 as it moves with the piercing mechanism 32, facilitating the rotating bearing 352 to smoothly reach the most protruding portion of the protrusion 622.

[0248] When working, Figure 15 As shown, when the piercing mechanism 32 moves down to the position where the protrusion 622 of the opening member 621 is located, the rotating bearing 352 of the opening mechanism 35 provided on the piercing mechanism 32 will squeeze the two opening members 621 apart, so that the two opening members 621 drive the two temperature control modules 61 to separate, making it easier for the amplification chamber 205a of the microfluidic chip 20 to be inserted between the heat transfer sheets 611b of the two temperature control modules 61; and as shown in FIG. Figure 16 As shown, when the piercing mechanism 32 continues to move downward and the rotating bearing 352 of the displacing mechanism 35 reaches below the protrusion 622, the two temperature control modules 61 and the two opening members 621 move closer to each other under the action of the temperature control elastic member 612a and the return elastic member 623, until the heat transfer plates 611b of the two temperature control modules 61 are in contact with the amplification chamber 205a inserted therein. Under the action of the temperature control elastic member 612a and the return elastic member 623, the two temperature control modules 61 can be closely attached to the amplification chamber 205a, which is conducive to achieving better temperature control effect and improving temperature control efficiency.

[0249] It can be seen that the opening mechanism 62 provided makes it easier to realize the opening function.

[0250] In summary, the detector 10 of this embodiment integrates multiple functional modules such as the holding device 2, the piercing device 3, the rotary valve device 4, the pump assembly 5, the temperature control device 6, the ultrasonic device 7, the magnetic device 8, the detection device 91 and the monitoring device 92, so as to realize fully automated control of the entire nucleic acid detection flow. In particular, since the rotary valve device 4, the prying mechanism 35 and the connecting device 5a are integrated on the piercing device 3 to form the piercing assembly 101, and the relative position relationship and the mutual movement relationship of the piercing assembly 101, the holding device 2, the temperature control device 6, the ultrasonic device 7 and the magnetic device 8 are reasonably designed, the structure is simple, the layout is compact, the volume is small and the efficiency is high.

[0251] The detector 10 of this embodiment is easy to use and has low operator requirements. The operator only needs to perform the following three steps to perform a fully automatic full-process detection:

[0252] (1) Adding a sample to the microfluidic chip 20;

[0253] (2) Place the microfluidic chip 20 on the holding device 2 and touch the "Start" button on the display screen;

[0254] (3) After the detection is completed, the microfluidic chip 20 is sent out of the cavity by the holding device 2, and the operator can take out the microfluidic chip 20.

[0255] Specifically, the working process of the detector 10 of this embodiment mainly includes four steps: microfluidic chip loading, nucleic acid extraction, nucleic acid amplification and detection, and microfluidic chip removal.

[0256] Among them, the microfluidic chip loading steps mainly include: the operator touches the "out of the warehouse" button on the display screen to move the holding device 2 outward into position, the operator installs the microfluidic chip 20 on the holding device 2, and touches the "start" button on the display screen to move the holding device 2 in the opposite direction, driving the microfluidic chip 20 to move backward to reach the target position in the instrument to cooperate with other modules, thereby completing the loading of the microfluidic chip 20.

[0257] The nucleic acid extraction steps mainly include the following processes:

[0258] (1) When the microfluidic chip 20 moves toward the target position, the piercing motor 331 of the piercing assembly 101 works to drive the piercing mechanism 32 to move downward, and stops moving downward when the separating mechanism 35 on the piercing mechanism 32 separates the two temperature control modules 61 of the temperature control device 6 that were originally attached to each other, so as to facilitate the amplification chamber 205a of the microfluidic chip 20 to enter between the two temperature control modules 61. After the microfluidic chip 20 is in place, the two temperature control modules 61 approach each other under the action of elastic force and automatically attach to the amplification chamber 205a.

[0259] (2) After the microfluidic chip 20 is in place, the piercing mechanism 32 continues to move downward, and the piercing member 322 breaks the connecting top cover 204 of the microfluidic chip 20 and the connecting rib 204d of the piercing needle 204c, so that the piercing needle 204c falls and breaks the sealing film on the storage cavity 201a of the microfluidic chip 20, so that the storage cavity 201a containing the reagent is in communication with the atmosphere;

[0260] (3) After the connecting rib 204d is broken, the piercing mechanism 32 continues to move downward until the valve shaft 411 provided on the piercing mechanism 32 is sleeved on the valve rod 203b of the microfluidic chip 20, and the communication nozzle 52 provided on the piercing mechanism 32 is inserted into the communication port 204b of the microfluidic chip 20, and after the microfluidic chip 20 is in communication with the driving pump 51, the downward movement is paused, the valve motor 431 of the valve device 4 works, the valve rod 203b is rotated by the belt transmission mechanism 433 and the valve shaft 411 according to a specific time sequence and angle, so that different storage cavities 201a are switched to be in communication with the reaction cavity 202a, and when the storage cavity 201a is in communication with the reaction cavity 202a, the fluid in the storage cavity 201a is sucked into the reaction cavity 202a by the suction action of the driving pump 51 to perform reaction; during the connection of the valve shaft 411 and the valve rod 203b and the communication nozzle 52 and the communication port 204b, the piercing mechanism 32 presses on the annular member 204h, so that the rib 204f between the annular member 204h and the stop member 204e is deformed, but the rib 204f connecting the annular member 204h and the stop member 204e is not completely broken, so the stop member 204e still prevents the piercing needle 204c from continuing to move downward, so that the piercing needle 204c can remain in the position of piercing the sealing film and communicating the storage cavity 201a with the atmosphere, and at the same time, the stop member 204e does not cause the piercing mechanism 32 to completely move downward, but the deformation of the rib 204f meets the corresponding downward movement requirement of the piercing mechanism 32;

[0261] (4) During the reaction process, when the magnetic beads and the reagent in the reaction cavity 202a need to be mixed, the ultrasonic transducer (not shown in the figure) of the ultrasonic device 7 presses against the outer wall of the reaction cavity 202 to apply vibration, so that the magnetic beads and the reagent are mixed to assist nucleic acid extraction;

[0262] (5) During the reaction process, after the magnetic beads and the reagent are mixed each time, waste liquid needs to be discharged, so that the ultrasonic transducer of the ultrasonic device 7 is away from the reaction cavity 202a, and then the magnetic attraction device 8 works, so that the magnet (not shown in the figure) of the magnetic attraction device 8 contacts the reaction cavity 202a to adsorb the magnetic beads on the inner wall of the reaction cavity 202a, and then the waste liquid is discharged to the outside of the reaction cavity 202a under the extrusion action of the driving pump 51;

[0263] (6) After the nucleic acid extraction is completed under the cooperation of the piercing assembly 101, the ultrasonic device 7, the magnetic suction device 8, and the driving pump 51, first, the valve motor 431 works to drive the valve rod 203b to rotate, so as to connect the reaction cavity 202a and the amplification cavity 205a, and under the extrusion of the driving pump 51, the nucleic acid extraction liquid flows into the amplification cavity 205a, then the piercing mechanism 32 continues to move downward, the rib 204f of the microfluidic chip 20 is pressed to break, so that the piercing needle 204c of the microfluidic chip 20 and the cover plate 26 together reseal the storage bin 201a, and preparation is made for amplification and fluorescence detection.

[0264] In the process of the nucleic acid extraction liquid flowing into the amplification cavity 205a and the piercing needle 204c and the cover plate 26 together resealing the storage bin 201a, the rotary valve shaft 411 and the connecting nozzle 52 continue to move downward with the piercing mechanism 32, and in the corresponding downward movement process, the rotary valve shaft 411 and the connecting nozzle 52 will not be damaged, wherein the connecting nozzle 52 can be moderately moved up and down under the action of the elastic member 55 to avoid damage, and the rotary valve shaft 411 can be improved in strength by using a material with higher strength and the like to prevent damage. In addition, in the corresponding downward movement process, since the liquid transfer steps in the entire detection process have been completed, even if the rotary valve shaft 411 and the connecting nozzle 52 continue to move downward in this process and cause damage to the switching valve 203 and the connecting port 204b of the microfluidic chip 20, it will not affect the subsequent process, and the entire detection process can still be completed smoothly. In fact, in the corresponding downward movement process, the rotary valve shaft 411 and the connecting nozzle 52 cause damage to the switching valve 203 and the connecting port 204b, which is expected, because in this way, the microfluidic chip 20 cannot be reused without affecting the smooth completion of the detection, so as to avoid problems caused by the reuse of the microfluidic chip 20.

[0265] The nucleic acid amplification and detection steps mainly include the following processes:

[0266] (1) After the nucleic acid extraction liquid flows into the amplification cavity 205a, the semiconductor refrigerating sheet 611a of the temperature control device 6 starts to circulate and heat, and the heat is transmitted to the amplification cavity 205a through the heat transmission sheet 611b to provide suitable and stable temperature conditions for nucleic acid amplification, and in this process, the heat dissipation fan 613b performs heat dissipation;

[0267] (2) At the same time of amplification, the detection device 91 works to repeatedly switch the fluorescence detection channel to detect the excited fluorescence in the amplification cavity 205a in real time;

[0268] (3) After the amplification and detection are completed, a detection report is generated, and a detection record is stored.

[0269] The microfluidic chip 20 removal step mainly includes the following two processes:

[0270] (1) After the detection, the microfluidic chip is discharged from the detection instrument: first, the motors of the piercing assembly 101, the ultrasonic device 7 and the magnetic attraction device 8 are operated, the piercing member 322, the ultrasonic transducer of the ultrasonic device 7 and the magnet of the magnetic attraction device 8 are moved away from the microfluidic chip 20, the motor of the holding device 2 is operated to drive the microfluidic chip 20 to move outward and be discharged from the detection instrument. During the process, when the piercing mechanism 32 moves upward to the pushing mechanism 35 to separate the two temperature control modules 61, the upward movement is paused, the motor of the holding device 2 is started to drive the microfluidic chip 20 to be discharged from the detection instrument, and after the amplification cavity 205a is completely withdrawn from the two temperature control modules 61, the piercing mechanism 32 continues to rise to make the two temperature control modules 61 adhere to each other again.

[0271] (2) After the microfluidic chip 20 is discharged from the detection instrument and reaches the designated position, the operator takes out the microfluidic chip 20 and places it in a specific collection box for further processing. If the second sample needs to be detected, the above steps are repeated. If the detection is not continued, the operator touches the “inlet” button on the display screen, and the holding device 2 stops when it reaches the starting position. The instrument sends a signal that it can be powered off, and the detection instrument 10 is turned off after the operator powers it off.

[0272] In the above steps, the actions of each component of the detection instrument 10 can be completed under the control of the controller of the detection instrument 10.

[0273] It can be seen that the detection instrument 10 of the embodiment has the advantages of high integration, automation and wide application range, which is conducive to promoting the on-site rapid detection of nucleic acids.

[0274] The above description is only an exemplary embodiment of the present application and does not limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A piercing assembly (101), characterized in that: include: A piercing device (3) comprises a piercing support (31), a piercing mechanism (32) and a piercing drive mechanism (33), wherein the piercing mechanism (32) is movably arranged on the piercing support (31), and the piercing drive mechanism (33) is drivingly connected to the piercing mechanism (32) to drive the piercing mechanism (32) to move relative to the piercing support (31), and during the movement of the piercing mechanism (32) relative to the piercing support (31), the piercing needle (204c) is driven to move relative to the storage cavity (201a) of the microfluidic chip (20) mounted on the holding device (2), thereby piercing the sealing membrane on the storage cavity (201a); Furthermore, the piercing assembly (101) further comprises at least one of the following: A rotary valve member (41) is provided on the piercing mechanism (32) and is connected to a rotor (203a) of a switching valve (203) of the microfluidic chip (20) during movement of the piercing mechanism (32) to drive the rotor (203a) to rotate, thereby switching the valve position of the switching valve (203); The pushing member (351) is provided on the piercing mechanism (32) and separates the two temperature control modules (61) of the temperature control device (6) during the movement of the piercing mechanism (32), so that the temperature-controlled portion (207) of the microfluidic chip (20) moves in and out between the two temperature control modules (61); The communication nozzle (52) is provided on the piercing mechanism (32) and is connected to the communication port (204b) of the microfluidic chip (20) during the movement of the piercing mechanism (32) to connect the communication port (204b) with the driving pump.

2. The piercing assembly (101) according to claim 1, characterized in that The rotary valve component (41) includes a rotary valve shaft (411), which is used to be sleeved outside the valve stem (203b) of the rotor (203a) and realize the switching of the valve position of the switching valve (203) by driving the valve stem (203b) to rotate.

3. The piercing assembly (101) according to claim 2, characterized in that The inner hole of the rotary valve shaft (411) for cooperating with the valve stem (203b) is a polygonal hole.

4. The piercing assembly (101) according to claim 2, characterized in that The piercing mechanism (32) includes a piercing member (322). When the piercing member (322) moves relative to the piercing bracket (31), it acts on the microfluidic chip (20), causing the piercing needle (204c) and the storage chamber (201a) to move relative to each other. The piercing member (322) is sleeved on the outside of the rotary valve member (41).

5. The piercing assembly (101) according to claim 4, characterized in that One end of the rotary valve member (41) close to the holding device (2) protrudes from one end of the piercing member (322) close to the holding device (2).

6. The piercing assembly (101) according to claim 5, characterized in that The distance between one end of the rotary valve member (41) close to the holding device (2) and one end of the piercing member (322) close to the holding device (2) is smaller than the distance between the top end of the rotor (203a) and the plane where the connecting rib (204d) of the microfluidic chip (20) is located.

7. The piercing assembly (101) according to claim 1, characterized in that The piercing assembly (101) includes a rotary valve driving mechanism (43), which is drivingly connected to the rotary valve member (41) to drive the rotary valve member (41) to drive the rotor (203a) to rotate.

8. The piercing assembly (101) according to claim 7, characterized in that The rotary valve driving mechanism (43) is arranged on the piercing mechanism (32).

9. The piercing assembly (101) according to any one of claims 1 to 8, characterized in that: The pushing member (351) is rotatably arranged on the piercing mechanism (32).

10. The piercing assembly (101) according to any one of claims 1 to 8, characterized in that: The piercing assembly (101) comprises a restraining member (54) and an elastic member (55), wherein the restraining member (54) supports the communicating mouth (52), and the elastic member (55) is sleeved on the outside of the communicating mouth (52) and abuts against the restraining member (54), thereby applying an elastic force to the communicating mouth (52) toward the communicating port (204b).

11. The piercing assembly (101) according to any one of claims 1 to 8, characterized in that: The piercing mechanism (32) breaks the connecting rib (204d) of the connecting piercing needle (204c) of the microfluidic chip (20), thereby causing the piercing needle (204c) to pierce the sealing film on the storage cavity (201a).

12. A detector (10), comprising a holding device (2), wherein the holding device (2) is used to hold a microfluidic chip (20), characterized in that: It also comprises a piercing assembly (101) according to any one of claims 1 to 11, wherein the piercing mechanism (32) of the piercing assembly (101) is movably arranged relative to the holding device (2).

13. The detector (10) according to claim 12, characterized in that The holding device (2) is arranged to be horizontally movable, and the piercing mechanism (32) is arranged to be vertically movable.

14. The detector (10) according to claim 12, characterized in that The detector (10) includes a temperature control device (6), and the temperature control device (6) includes two temperature control modules (61). The two temperature control modules (61) are opposite to each other and can move relative to each other. When the temperature-controlled part (207) of the microfluidic chip (20) is inserted between the two temperature control modules (61), the two temperature control modules (61) perform temperature control on the temperature-controlled part (207). The separating member (351) separates the two temperature control modules (61).

15. The detector (10) according to claim 14, characterized in that The temperature control device (6) comprises two opening members (621), the two opening members (621) being connected to the two temperature control modules (61) in a one-to-one correspondence, and the opening member separates the two temperature control modules (61) by pushing the two opening members (621) away from each other.

16. The detector (10) according to claim 15, characterized in that The opening member (621) includes a convex portion (622), and the pushing member pushes the two opening members away from each other by acting on the convex portion (622).

17. The detector (10) according to claim 15, characterized in that The surface of the opening member (621) for contacting the spreading member is arc-shaped.

18. The detector (10) according to claim 15, characterized in that The temperature control device (6) comprises a reset elastic member (623), the reset elastic member (623) abuts against the opening member (621), and applies an elastic force to the opening member (621) so as to bring the two opening members (621) closer to each other.

19. A detection system (100), comprising a microfluidic chip (20), characterized in that: It comprises a detector (10) as described in any one of claims 12-18.

20. The detection system (100) according to claim 19, characterized in that Along the direction in which the rotary valve shaft (411) of the detector (10) approaches the holding device (2), the cross-sectional size of the inner hole of the rotary valve shaft (411) remains unchanged, and the cross-sectional size of the valve stem (203b) of the microfluidic chip (20) becomes larger; or, the cross-sectional size of the valve stem (203b) of the microfluidic chip (20) remains unchanged, and the cross-sectional size of the inner hole of the rotary valve shaft (411) becomes smaller, so that the connection tightness between the rotary valve shaft (411) and the valve stem (203b) becomes larger along the direction in which the rotary valve shaft (411) approaches the holding device (2).

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