Process cartridge

Abstract

The invention provides a treatment cartridge (1) comprising a main cartridge section (2) and an actuation cartridge section (3), the main cartridge section (2) comprising a liquid reservoir (4), a liquid container (5), a container piercing element (6) and a resilient container retaining element (9), wherein the actuation cartridge section (3) is configured to interface with the main cartridge section (2) and comprises an actuation member (10) configured to interact with the liquid container (5) during the interface; the liquid container, the piercing element (6) and the container retaining element (9) are arranged in the liquid reservoir (4), the liquid container is filled with a suitable treatment liquid and features a pierceable dispensing side (16), and the container piercing element faces the dispensing side; the liquid container (5) is retained in a first position by the container retaining element, wherein the dispensing side is separated from the container piercing element; and the actuation member (10) is configured to move the liquid container from the first position to a second position during the interface of the cartridge actuation section, and in the second position the dispensing side (16) is pierced by the container piercing element.

Description

Technical Field

[0001] This invention relates to the field of processing cartridges, and more specifically, to processing cartridges for sample analysis and components for providing samples and releasing processing liquids into the processing cartridges. Background Technology

[0002] Centrifugation has long been an integral part of chemical and biochemical processes as a means of accelerating the sedimentation of cells, particles, and precipitates, as well as separating liquids or cells of different densities. Furthermore, the use of centrifugation for chemical reactions and analyses in microfluidic loops arranged in processing cassettes or chips is a well-known technique; see, for example, “A review of biomedical centrifugal microfluidic platforms” published by Tang et al. in Micromachines on July 26, 2016.

[0003] In most analytical systems that use processing cartridges, the direction of the centrifugal force applied to the microfluidic circuit is constant, and centrifuges similar to optical disc (CD) players are often used, in which the microfluidic circuit is arranged in a CD-shaped chip or cartridge. Typical centrifuges for such analytical systems are disclosed, for example, in JP 2018-163169A and US2018 / 0003732A1.

[0004] Other analytical systems utilize biaxial centrifuges capable of providing variable centrifugal forces, as disclosed, for example, in US 4814282, WO2011 / 081531A1, and WO 2019 / 091650 A1. Biaxial centrifuges can provide a more flexible and faster means of processing in microfluidic loops.

[0005] A common operational feature of the processing kit is the ability to introduce samples into the microfluidic circuit in a repeatable and safe manner. Additionally, processing liquids are typically required to dissolve, dilute, clean, and / or transfer samples and / or various reactants within the microfluidic circuit of the processing kit.

[0006] EP 2881743 A1 discloses a processing box in which a sample is introduced into the box via an insertion-type sample box. The sample box is characterized by a member that moves a liquid container within the processing box during insertion. This movement releases liquid from the liquid container simultaneously with the introduction of the sample.

[0007] US2011 / 0117665 A1 discloses a processing cartridge characterized by a pivotally connected arm for holding a liquid container within the cartridge stationary before use. During use, the arm pivots away from the rest of the cartridge to provide an inlet for the sample collector and to release the liquid container. After sampling, the arm pivots back toward the cartridge, simultaneously pushing the liquid container toward the tip to release the liquid.

[0008] The object of this invention is to provide a treatment cartridge in which a treatment liquid can be stored and released in an improved manner. Another object of this invention is to provide a treatment cartridge for improved sampling and treatment during use. Summary of the Invention

[0009] This invention is defined by the appended claims and the following:

[0010] In a first aspect, the present invention provides a processing box comprising a main box section and an actuating box section, wherein the main box section includes a liquid storage chamber, a liquid container, a container piercing element, and a resilient container holding element, wherein...

[0011] - The actuating box section is configured to dock with the main box section and includes an actuating member configured to interact with the liquid container during docking;

[0012] - A liquid container, a puncture element, and a container holding element are arranged in a liquid storage chamber. The liquid container is filled with a suitable processing liquid and features a punctureable dispensing side, and the container puncture element faces the dispensing side.

[0013] - The liquid container is held in a first position by a container holding element, wherein the dispensing side is separated from the container piercing element; and

[0014] - The actuating member is configured to move the liquid container from a first position to a second position during docking of the box actuating section, and in the second position, the dispensing side is pierced by the container piercing element.

[0015] The dispensing side can include any suitable puncture-resistant and fluid-tight material, such as aluminum and / or plastic foil. The dispensing side may also be referred to as a puncture-resistant sealing cap or a puncture-resistant sealing foil.

[0016] In other words, the side of the component is positioned to pierce the container.

[0017] The actuating element can be configured to move the liquid container through direct or indirect interaction with it. The movement of the liquid container overcomes forces from the elastic container holding element. During the movement of the liquid container, the dispensing side is pushed against the container piercing element, allowing the processed fluid to be released into the liquid storage chamber.

[0018] In one embodiment of the processing box, the actuation box segment may include a first end portion pivotally connected to the main box segment, such that the actuation box segment can pivot between a non-dating position and a mating position, in which the liquid container is held in a first position by a container holding element, and in the mating position, the actuation box segment is mated to the main box segment and the liquid container is in a second position.

[0019] The container holding element may include any suitable resilient element capable of holding the liquid container in a first position and allowing an actuating member to move the liquid container to a second position. The container holding element may be configured to provide a resilient force acting on the liquid container and holding it in the first position. Movement of the liquid container from the first position must overcome the resilient force acting on the liquid container. The container holding element may be configured to deflect away from the liquid container during movement toward the second position.

[0020] Container holding elements may include at least one resilient arm that comes into contact with the liquid container. The resilient arm may also be referred to as a resilient rod.

[0021] A resilient arm or lever may be arranged to contact the edge of a liquid container and to deflect away from that edge when an actuating member moves the liquid container to a second position. A portion of the arm / lever that contacts the edge of the liquid container may be tilted relative to the direction in which the liquid container is moved by the actuating member. The tilted surface provides smooth and uniform movement of the liquid container. The resilient arm / lever may include a curved portion having opposing first and second sides, wherein the first side contacts the liquid container and is pushed towards the second side as the liquid container moves toward the second position.

[0022] In one embodiment of the processing box, the actuating member is configured to enter the liquid storage chamber during docking.

[0023] In an embodiment of the processing box, the main box section may include a box base and a top cover.

[0024] In one embodiment of the processing box, the liquid storage chamber includes sidewalls and a base surface. The sidewalls and base surface may be disposed within the box base. The liquid storage chamber may also be defined by a top surface. The top surface may be provided by a top cover.

[0025] In an embodiment of the processing cartridge, the liquid container may include a recess. An actuating member may be configured to interact with an edge of the recess when entering the liquid reservoir during docking. The recess may include: an edge through which the actuating member may push the liquid container toward or move the liquid container toward the container piercing element; and at least one side surface that may interact with the actuating member to restrict lateral movement of the liquid container. The restricted lateral movement may be relative to the plane through which the liquid container is pushed or moved, and / or relative to the base surface of the liquid reservoir. The side surfaces of the recess may be configured such that the actuating member can prevent lateral movement of the liquid container away from the base surface.

[0026] The actuating element can be wing-shaped, i.e., an actuating wing. The portion of the actuating element that interacts with the liquid container can have a circular periphery to achieve a smooth and uniform interaction with the edge of the recess.

[0027] The interaction between the actuating member and the recess in the liquid container provides stable movement of the liquid container toward the puncturing element and prevents lateral movement of the liquid container relative to the actuating member.

[0028] In one embodiment, the processing box may include a sample collector arranged to be accessible to collect samples when the actuation box section is separated from the main box section or in a non-dating position.

[0029] A sample collector can be any element suitable for obtaining solid, semi-solid, mucus, or liquid samples from a subject or object. A sample collector can be a capillary tube for collecting liquid samples, such as whole blood, or a swab for collecting semi-solid / mucus samples.

[0030] In one embodiment of the processing box, the sample collector can be arranged on the actuation box section such that the sample collector is contained in the sample receiving cavity in the main box section when the actuation box section is docked with or in the docking position.

[0031] The actuator box section can be a longitudinal section.

[0032] In one embodiment of the processing box, the actuation box section may include a second end at which the sample collector is disposed.

[0033] In an embodiment of the processing box, the main box segment may be characterized by at least one guide rib or recess, which is configured to interact with a corresponding at least one recess or guide rib in the actuation box segment during docking. The guide rib may be characterized by a tapered edge for insertion into the recess.

[0034] The guide rib / recess can be arranged on the peripheral section of the main box section, and the corresponding recess / guide rib can be arranged on the side of the actuation box section facing the main box section during docking.

[0035] In embodiments of the processing box, the main box section or the actuation box section may be characterized by a locking rib having a locking recess disposed on its opposite side, and the corresponding actuation box section or the main box section may be characterized by two opposing resilient hooks, each hook configured to interact with a corresponding locking recess on the locking rib, wherein the locking rib has a tapered edge configured to be inserted between the resilient hooks during docking.

[0036] Compared to using a single hook to provide the same reliable locking, a solution with two opposing hooks—with a locking rib inserted between them—allows for less hook deflection. Less deflection ensures a more uniform and smoother locking motion. However, in alternative implementations, the locking rib may be characterized by a single recess for interaction with the single hook, which may be, for example, located at the end of the locking rib.

[0037] In embodiments of the processing cartridge, the container piercing element may include a base portion, at least one side portion, and a sharp portion away from the base portion. The sharp portion, such as a pointed tip, may be arranged toward the dispensing side of the liquid container.

[0038] In embodiments of the processing box, the container piercing element may include at least one recess disposed in the side portion. The recess may extend between the base portion and the pointed portion.

[0039] In the implementation of the processing box, the container piercing element and the container retainer element constitute the parts of a single unit.

[0040] In one embodiment of the processing box, the liquid storage chamber includes sidewalls and a base surface characterized by a protrusion, and a single unit is secured within the liquid storage chamber by arranging a portion of the single unit between the protrusion and the sidewall.

[0041] In the embodiment of the processing box, the actuation box section can be connected to the main box section via a pivot connection, wherein...

[0042] - The actuating box section or main box section is characterized by including a first end of two opposing clamping arms and a resilient arm arranged between the clamping arms, each of the clamping arms being characterized by a pivoting protrusion, and the pivoting protrusions being arranged opposite to each other (i.e. facing each other); and

[0043] -The corresponding main box section or actuating box section is characterized by a connecting hub for insertion between two clamping arms, the connecting hub including a hole for receiving a pivoting protrusion and having an outer periphery for interacting with the elastic arm;

[0044] The first segment of the outer periphery has a shorter distance from the center line of the hole compared to the second segment of the outer periphery. The first and second segments of the outer periphery are connected by the third segment of the outer periphery, which has an increased distance from the center line of the hole. The third segment of the outer periphery is arranged such that the movement of the actuating box segment when the elastic arm interacts with the third segment will face limited resistance.

[0045] In other words, when the pivoting motion of the actuation box section causes the elastic arm to interact with the third section, the motion of the actuation box section will face a defined resistance. This defined resistance can be uniform, variable, or controllable.

[0046] In other words, the distance from the third segment of the outer periphery to the center line of the hole increases from the distance equal to that of the first segment to the distance of the second segment.

[0047] The aperture may have an inner circular periphery arranged to accommodate a pivoting protrusion. The pivoting protrusion has an outer periphery, at least two opposite segments of which mate with the inner circular periphery of the aperture. The two opposite segments may be radial or curved segments. When accommodated in the aperture, the common centerline of the two pivoting protrusions coincides with the centerline of the aperture. The opposite segments ensure that the common centerline of the two pivoting protrusions coincides with the centerline of the aperture.

[0048] In the embodiment of the processing box, the actuation box section can be connected to the main box section via a pivot connection, wherein...

[0049] - The actuating box section or main box section is characterized by including a first end of two opposing clamping arms, each of the clamping arms being characterized by a pivoting protrusion, and the pivoting protrusions being disposed opposite to each other; and

[0050] - The corresponding main box section or actuation box section is characterized by a connecting hub for insertion between two clamping arms, the connecting hub including a hole for receiving a pivoting protrusion, the hole being defined by a middle section having a circular inner wall and a first section and a second section arranged on opposite sides of the middle section, each of the first section and the second section including a recess extending between the hole and the outer periphery of the connecting hub.

[0051] - Each pivoting protrusion has an outer periphery comprising two opposing first segments and two opposing second segments configured to mate with a circular inner wall, wherein the distance between the second segments is shorter than the maximum distance between the first segments, and each recess has a width smaller than the maximum distance between the first segments and greater than the maximum distance between the second segments.

[0052] The pivoting protrusions and recesses are sized and configured such that the connecting hub can be inserted between the clamping arms with minimal deflection when each pivoting protrusion in the pivoting protrusion is oriented through a first end of the first segment facing the corresponding recess.

[0053] The first segment can be a radial or curved segment, and the second segment can be a parallel straight segment.

[0054] In an embodiment of the processing cartridge, the main cartridge section may include a microfluidic circuit that can be connected to the sample receiving chamber and the liquid storage chamber.

[0055] In the implementation of the processing box, when the actuation box section is in the docking position, the main box section and the actuation box section can define the disc-shaped body.

[0056] In a second aspect, the present invention provides a processing box, the processing box including a main box section and an actuation box section, the main box section including a liquid storage chamber, a liquid container and a container piercing element, wherein...

[0057] - The actuating box section is configured to dock with the main box section and includes an actuating member configured to interact with the liquid container during docking;

[0058] - A liquid container and a piercing element are arranged in a liquid storage chamber, the liquid container being filled with a suitable processing liquid and characterized by a pierceable dispensing side, and the container piercing element facing the dispensing side.

[0059] - The liquid container is in the first position before docking, wherein the dispensing side is separated from the container piercing element; and

[0060] - The actuating member is configured to move the liquid container from a first position to a second position during docking of the box actuating section, and in the second position, the dispensing side is pierced by the container piercing element.

[0061] The second aspect of the invention may include any of the features of the embodiments of the first aspect.

[0062] In a third aspect, the present invention provides a processing box, the processing box including a main box section and an actuation box section, the main box section including a liquid storage chamber, a liquid container, a container holding element, and a container piercing element, wherein...

[0063] - The actuating box section is configured to dock with the main box section and includes an actuating member configured to interact with the liquid container during docking;

[0064] - A liquid container and a piercing element are arranged in a liquid storage chamber, the liquid container being filled with a suitable processing liquid and characterized by a pierceable dispensing side, and the container piercing element facing the dispensing side.

[0065] - The liquid container is held in a first position by a container holding element, wherein the dispensing side is separated from the container piercing element; and

[0066] - The actuating member is configured to move the liquid container from a first position to a second position during docking of the box actuating section, and in the second position, the dispensing side is pierced by the container piercing element.

[0067] The third aspect of the invention may include any of the features of the embodiments of the first aspect.

[0068] The actuating components of the first, second, and third aspects can be configured to move the liquid container through direct or indirect interaction with it.

[0069] In a fourth aspect, the present invention provides a method for introducing a sample into a processing box, the processing box comprising a main box section, an actuation box section, and a sample collector, wherein the main box section includes a liquid storage chamber, a liquid container, a container piercing element, and a resilient container holding element, wherein...

[0070] The actuation box section is configured to dock with the main box section and includes a first end and an actuation member, the first end being pivotally connected to the main box section, and the actuation member being configured to interact with a liquid container during docking;

[0071] A liquid container and a piercing element are arranged in a liquid storage chamber. The liquid container is filled with a suitable processing liquid and features a pierceable dispensing side, and the container piercing element faces the dispensing side.

[0072] The actuator section can be arranged in a non-dock position, in which the liquid container is held in a first position by a container holding element, in which the dispensing side is separated from the container piercing element, and the sample collector can be used to collect samples; and the method includes the following steps:

[0073] - Keep the actuator box section in the non-dating position;

[0074] - Collect samples using a sample collector; and

[0075] - The actuation box section is pivoted to the docking position, in which the actuation member has moved the liquid container from the first position to the second position, in which the dispensing side is pierced by the container piercing element.

[0076] In an embodiment of the method, the main box section includes a sample cavity, and when the actuation box section pivots to the docking position, the sample collector is introduced into the sample cavity.

[0077] In one embodiment of the processing box, the internal volume of the main box section includes a microfluidic circuit for processing samples, such as for analyzing samples.

[0078] The term "processing kit" is intended to refer to a chip / kit that includes an internal fluid circuit suitable for analyzing, testing, and performing various types of chemical reactions, such as polymerase chain reaction (PCR) and synthetic chemical reactions, on suitable samples introduced into the kit. Attached Figure Description

[0079] Embodiments of the invention will now be described in more detail by way of example only and with reference to the following figures:

[0080] Figure 1 This is a perspective view of an exemplary processing box according to the present invention.

[0081] Figure 2 yes Figure 1 A perspective view of the processing box in which the top foil has been removed.

[0082] Figure 3 yes Figure 1 An exploded view of the processing box in the diagram.

[0083] Figure 4 yes Figure 1 A three-dimensional side view of the processing box, in which the sampling arm is in a non-docked position.

[0084] Figure 5 yes Figure 1 A three-dimensional side view of the processing box, in which the sampling arm is in the docking position.

[0085] Figures 6a to 6c yes Figure 1 A detailed side view of the processing box in the image.

[0086] Figure 7 yes Figure 1 Side view of the processing box in the middle.

[0087] Figure 8 is Figure 7 A cross-sectional view of the processing box in the image.

[0088] Figure 9 A perspective view is shown of a unit characterized by a pointed portion and an elastic arm for use in a processing box according to the invention.

[0089] Figure 10 A perspective view and an exploded view of a liquid container for use in a processing box according to the invention are shown.

[0090] Figure 11 This is a perspective view of a sampling arm used in a processing box according to the invention.

[0091] Figure 12 yes Figure 1 A stereoscopic side view of the processing box without the sampling arm.

[0092] Figure 13 Details of the pivoting connection for the processing box according to the invention are shown.

[0093] Figure 14 and Figure 15 It shows Figure 13 Details of the central connecting section.

[0094] Figures 16a to 1 6c shows Figure 13 Details of the central connecting section.

[0095] Figures 17a and 17b show details of the locking mechanism for the processing box according to the invention. Detailed Implementation

[0096] The object of the present invention is to provide a processing cartridge suitable for use in centrifuge-based analytical systems as described in the background section.

[0097] exist Figure 1 and Figure 2 An exemplary processing box according to the present invention is shown in the figure.

[0098] The processing box 1 includes a main box section 2 and a sampling arm 3 (i.e., an actuation box section).

[0099] The main box section 2 is characterized by an internal fluid or microfluidic circuit 7. For illustrative purposes, in Figures 1 to 3An exemplary fluidic circuit 7 is shown in the cartridge. The fluidic circuit of the processing cartridge can be designed for processing samples, such as performing various types of analytical tests on whole blood samples, or for processing various chemical reactions, including polymerase chain reaction (PCR) and synthetic chemical reactions. The processing cartridge may, for example, include microchannels and various cavities for manipulating, processing, and delivering various liquids in nL, L, and mL volumes. Furthermore, the cartridge can be optically transparent or translucent. This allows for the study of liquid delivery, color development, separation, etc., throughout the cartridge. Samples can be fluid, semi-solid, or solid, typically whole blood, plasma, serum, urine, mucus, tissue, or fecal samples. Semi-solid and solid samples are fluidized prior to processing in the fluidic circuit. Additionally, the cartridge may contain one or more reagents. An exemplary fluidic circuit that can be used in the processing cartridge according to the invention is disclosed, for example, in WO 2011 / 081530 A1, the contents of which are incorporated herein by reference. Furthermore, various methods that can be performed in such fluid circuits, as well as suitable fluid circuits, are disclosed in PCT / EP2015 / 063811, PCT / EP2015 / 063817 and PCT / EP2015 / 063824.

[0100] exist Figure 1 In the middle, the main box section 2 includes a box base 2a and a top cover 2b that closes the fluid circuit 7 in the box base 2a on one side. The top cover 2b can be, for example, a rigid plastic film layer. For clarity, in the remaining... Figures 4 to 1 Fluid circuit 7 and top cover 2b are omitted in 7.

[0101] exist Figure 3 An exploded view of processing box 1 is shown to illustrate the main components constituting the processing box. (Refer to...) Figures 4 to 1 7. Describe the function of the processing box and its various advantageous features.

[0102] As described above, the processing box consists of a main box section 2 and a sampling arm 3.

[0103] The main box section 2 is characterized by a liquid storage chamber 4, a liquid container 5, a pointed part 6 (i.e., a container piercing element) and an elastic arm 9 (i.e., an elastic container holding element).

[0104] The sampling arm 3 has a first end 13 pivotally connected to the main box section 2. The second end 14 of the sampling arm 3 is characterized by a capillary 15 (i.e., a sample collector) for collecting samples. The sampling arm 3 is configured to dock with the main box section 2. An actuating fin 10 (i.e., an actuating member) is arranged on the sampling arm 3, and the actuating fin 10 is configured to enter the liquid storage chamber 4 during docking of the sampling arm 3 with the main box section 2.

[0105] Liquid container 5, pointed portion 6, and flexible arm 9 are arranged in liquid storage chamber 4. See also Figure 10The liquid container 5 is filled with a suitable processing liquid and features a puncture-resistant dispensing side 16. Depending on the type of analysis to be performed and / or the type of sample to be analyzed, the processing liquid can be, for example, any suitable buffer and / or solvent. The dispensing side 16 is characterized by at least a portion comprising a puncture-resistant and liquid-impermeable foil 16a. The liquid-impermeable foil 16a can be made, for example, of aluminum, a suitable polymer material, or any combination thereof.

[0106] The pointed portion 6 is arranged to face the dispensing side of the liquid container 5. In this embodiment, see [reference needed]. Figure 9 The tip 6 includes a base portion 25, a side portion 26, and a pointed portion 27 away from the base portion. The side portion 26 may be characterized by a recess 28 extending between the base portion 25 and the pointed portion 27. When the dispensing side is pierced by the tip 6, the aluminum or polymer foil of the dispensing side may, in some cases, seal the tip, preventing the liquid from being released freely as desired. In such cases, the recess 28 will provide a more reproducible liquid release by breaking the potential seal of the foil on the side portion 26.

[0107] To achieve a simple method of securing the pointed portion 6 and the elastic arm 9 within the liquid storage chamber 4, the pointed portion 6 and the elastic arm 9 are interconnected via a connecting section 39, meaning that the pointed portion 6 and the elastic arm 9 form a single element or a single unit component. See [link to documentation]. Figure 9 The liquid storage chamber 4 is characterized by sidewalls 37 and a base surface 43 having protrusions 22, see, for example, [reference needed]. Figure 3 The pointed portion 6 and the elastic arm 9 are fixed in the liquid storage chamber 4 by arranging the connecting section 39 between the protrusion 22 and the side wall 37.

[0108] During use, the sampling arm is initially positioned in a non-docked location, such as... Figure 4 , Figure 15 As shown in Figure 16. In the non-docking position, the liquid container 5 is held in a first position by the elastic arm 9, such that the dispensing side 16 is separated from the tip 6. When the sampling arm is in the non-docking position, the capillary 15 can be used to collect a sample. This sample can be, for example, a whole blood sample from a patient. In this way, the sample can be collected while the liquid container is fixed and prevented from moving toward the tip. Due to the biasing force of the elastic arm 9, the liquid container remains in place even if the processing cartridge is subjected to violent movement during sample collection. The latter effect is very advantageous because even premature release of a small amount of liquid can affect subsequent sample analysis. Therefore, the secure positioning of the liquid container during sampling eliminates a possible cause of error in the analytical results.

[0109] To prevent accidental movement of the sampling arm relative to the main box section during sampling, the sampling arm is connected to the main box section via a pivoting connection that provides resistance to movement of the sampling arm from the non-abutting position. The pivoting connection is achieved by having two opposing clamping arms 30a, 30b at the first end 13 of the sampling arm 3 and a resilient arm 29 disposed between the clamping arms 30a, 30b. See [reference needed]. Figures 11 to 1 6. Each of the clamping arms is characterized by pivoting protrusions 31a, 31b, which are arranged opposite to each other. The main housing section 2 is characterized by a connecting hub 32 for insertion between the two clamping arms 30a, 30b into the sampling arm 3. The connecting hub 32 includes holes 33 for receiving the pivoting protrusions 31a, 31b and has an outer periphery for interacting with the resilient arm 29. A first section S1 of the outer periphery has a shorter distance R1 from the centerline C of the hole 33 than a second section S2 of the outer periphery. The first and second sections are interconnected by a third section S3 of the outer periphery, which has an increased distance C from the centerline of the hole, i.e., an increase from R1 to R2.

[0110] During the movement of the sampling arm from the non-docked position, the elastic arm 29 will exert a limited resistance to the movement as it interacts with the third segment of the outer periphery.

[0111] To increase the lateral stiffness of the pivoting connection, the bore 33 is defined by a central section 34a having a circular inner wall and a first section 34b and a second section 34c arranged on opposite sides of the central section. Each of the first and second sections includes a recess 35 extending between the bore 33 and the outer periphery of the connecting hub 32. Each pivoting protrusion 31a, 31b has an outer periphery comprising two opposite radial sections K1 and two opposite parallel sections K2 configured to mate with the circular inner wall, wherein the distance between the parallel sections K2 is shorter than the maximum distance between the radial sections K1, and each recess 35 has a width smaller than the maximum distance between the radial sections K1 and greater than the maximum distance between the parallel sections K2. The dimensions of the pivot protrusions 31a and 31b and the recess 35 are configured such that when each of the pivot protrusions 31a and 31b is oriented such that one of the radial segments K1 faces the corresponding recess 35, the connecting hub 32 can be inserted between the clamping arms 30a and 30b by minimal deflection of the clamping arms. Due to the minimal deflection of the clamping arms during connection to the connecting hub 32 via the recess 35, the stiffness of the clamping arms can be increased, and the lateral stiffness of the pivot connection is optimized.

[0112] In other embodiments, the main box section may be characterized by two opposing clamping arms as described above, while the connecting hub as described above is arranged on the first end of the sampling arm.

[0113] After the sample has been collected by capillary 15, the sampling arm pivots from the non-dock position to the docking position, see [reference]. Figure 5 and Figure 6c The capillary 15 is introduced into the sample chamber 17. The sample chamber 17 includes a sample inlet 38 connected to the fluid circuit 7 of the main box section.

[0114] When the sampling arm 3 is in the docking position, the liquid container is in the second position. In the second position, the dispensing side 16 of the liquid container has been punctured, and liquid can be released into the liquid storage chamber 4. The liquid storage chamber 4 includes a liquid outlet 36 through which the processed liquid can be transferred after being released from the liquid container.

[0115] See Figure 6b and Figure 7 During movement toward the docking position, the actuating fin 10 enters the liquid storage chamber 4 and contacts the liquid container 5. In the illustrated embodiment, the actuating fin 10 and the liquid container 5 are shown in the figure. Figure 10 The edges 11a of the recesses 11 arranged in the side of the liquid container 5 interact with each other. By interacting with the recesses 11, rather than with, for example, the top section of the liquid container, the actuating fins 10 help guide and stabilize the movement of the liquid container toward the tip 6. Thus, minor lateral movements of the liquid container 5 relative to its directional movement toward the tip 6 are avoided or limited. Otherwise, such lateral movements could hinder the smooth and uniform movement of the liquid container 5, potentially causing the liquid container to jam or resulting in insufficient or excessive piercing of the dispensing side. Another important advantage of limiting the lateral movement of the liquid container 5 is that it prevents the liquid container 5 from being pushed against the top cover 2b during its movement from the first position to the second position. The top cover 2b is laminated to the box base 2a, for example, by heating or by a suitable adhesive. The lamination between the top cover 2b and the box base 2a may have weak points at the top of the wall forming the fluid loop 7 in the box base 2a. Therefore, by preventing the liquid container 5 from being pushed against the top cover 2b, accidental detachment of the top cover 2b from the various parts of the box base 2a is avoided. Suitable alternative designs for the recesses are foreseeable and all include: an edge 11a through which the actuating fins 10 can push or move the liquid container 5 toward the tip 6; and at least one side surface 11b that interacts with the actuating fins 10 to limit lateral movement. The side surface 11b is configured such that the actuating fins can prevent lateral movement of the liquid container away from the base surface 43.

[0116] The resilient arm 9 is configured to deflect away from the edge of the liquid container as the liquid container 5 moves toward the pointed portion 6. This deflection may cause elastic and / or plastic deformation of the resilient arm, or even breakage. In an exemplary processing cassette, the resilient arm is characterized by a bent portion 40a and opposing first side portions 40b and second side portions 40c, wherein, when the liquid container 5 moves toward the second position, the first side portion 40b contacts the liquid container 5 and is pushed toward the second side portion 40c, see [reference needed]. Figure 9 The first side portion 40b has an inclined portion 40d that contacts the edge 41 of the dispensing side portion of the liquid container 5.

[0117] The processing cartridge 1 according to the invention may include features for limiting lateral movement of the sampling arm 3 during movement toward the docking position. This prevents movement of the sampling arm 3 in a direction perpendicular to the plane of pivoting movement toward the docking position. In this embodiment, the main cartridge section 2 includes a guide rib 18, and the sampling arm has a guide recess 19 into which the guide rib is introduced during pivoting movement of the sampling arm, see [reference needed]. Figure 7 And Figure 8. The guide rib 18 has a tapered edge 20 facing the guide recess 19, such that the lateral restraint provided by the interaction between the guide rib and the guide recess can be optimal, while ensuring smooth interaction during the movement of the sampling arm toward the docking position. By restricting or preventing lateral movement of the sampling arm, potential errors in the sampling process are reduced. Such errors can be caused by violent movement of the sampling arm, which may prematurely release some sample, for example, before the capillary enters the sample chamber 17. In other embodiments, the sampling arm 3 may include a guide rib, while the guide recess is arranged on the main housing section 2.

[0118] In the docking position, the sampling arm 3 is connected to the main housing section 2 via a locking mechanism. In an exemplary processing housing, the main housing section 2 is characterized by a locking rib 21 having locking recesses 23a, 23b arranged on opposite sides thereon, see Figures 17a and 17b. The locking rib 21 may be a continuation of the guide rib 18. The sampling arm 3 is characterized by two opposing resilient hooks 24a, 24b, each hook 24 configured to interact with a corresponding locking recess 23a, 23b on the locking rib. The locking rib 21 has a tapered edge 20' configured to insert between the resilient hooks 24 during docking of the sampling arm 3. The solution of having two opposing hooks—with the locking rib inserted between the two opposing hooks—allows for smaller deflection of the hooks 24a, 24b relative to a single hook located on one side of the locking rib, which is designed to provide equally secure locking. The smaller deflection ensures a more uniform and smoother movement of the sampling arm 3 during locking. The latter effect is advantageous because vigorous movement of the sampling arm is avoided or reduced. Vigorous movement could prematurely release some sample, for example, releasing the sample into the sample chamber 17 or from the top of the capillary 15 before the processing cartridge 1 is introduced into the centrifuge-based analysis system. In other embodiments, the sampling arm 3 may include locking ribs, while two opposing elastic hooks are arranged on the main cartridge segment 2.

[0119] In the exemplary processing cassette, the liquid container is held in a first position by a resilient arm 9. However, other suitable resilient members are conceivable and can take any form, as long as the member is capable of holding the liquid container in the first position preferably by an elastic force and allows the actuating fins to move the liquid container toward a second position against a biasing force. In other embodiments, the resilient arm 9 or any other suitable resilient member may be manufactured (e.g., molded) as an integral part of the liquid container 5 or the liquid storage chamber 4.

[0120] In the exemplary processing cartridge, the pointed portion 6 is molded separately from the cartridge base 2a. In other embodiments, the pointed portion 6 may be manufactured (e.g., molded) as an integral part of the liquid storage chamber 4.

[0121] The exemplary processing boxes illustrated and described herein include combinations of several highly advantageous features. However, in other embodiments of the invention, advantageous processing boxes may include any desired combination of the described features.

[0122] In an exemplary embodiment, the circumference of the processing box is generally circular. However, in other embodiments, the circumference of the processing box can have any suitable shape, such as rectangular, square, or elliptical.

Claims

1. A processing box (1), the processing box (1) comprising a main box section (2) and an actuation box section (3), the main box section (2) comprising a liquid storage chamber (4), a liquid container (5), a container piercing element (6), and a resilient container holding element (9), wherein - The actuation box section (3) is configured to dock with the main box section (2) and includes an actuation member (10) configured to interact with the liquid container (5) during docking. - The liquid container, the container piercing element (6) and the container holding element (9) are arranged in the liquid storage chamber (4), the liquid container is filled with a suitable processing liquid and is characterized by a piercing dispensing side (16), and the container piercing element faces the dispensing side; - The liquid container (5) is held in a first position by the container holding element, in which the dispensing side is separated from the container piercing element; and - The actuating member (10) is configured to move the liquid container from the first position to the second position during docking of the actuating box section, and in the second position, the dispensing side (16) is pierced by the container piercing element; The container holding element includes an elastic arm that contacts the edge of the liquid container (5), the elastic arm being arranged to deflect away from the edge when the actuating member moves the liquid container to a second position.

2. The processing box (1) according to claim 1, wherein, The actuation box section (3) includes a first end (13) which is pivotally connected to the main box section (2) such that the actuation box section (3) is pivotable between a non-dating position and a docking position, in which the liquid container is held in the first position by the container holding element, and in the docking position, the actuation box section (3) docks with the main box section and the liquid container is in the second position.

3. The processing box (1) according to claim 1 or 2, wherein, The container holding element is configured to provide an elastic force acting on the liquid container and holding the liquid container in the first position.

4. The processing box (1) according to claim 1 or 2, wherein, The actuating member is configured to enter the liquid storage chamber (4) during docking, and the liquid container (5) includes a recess (11), the actuating member (10) being configured to interact with the edge (11a) of the recess when entering the liquid storage chamber (4) during docking.

5. The processing box (1) according to claim 2, comprising a sample collector (15) arranged to be accessible for collecting samples when the actuation box section (3) is separated from the main box section or in the non-dating position.

6. The processing box (1) according to claim 5, wherein, The sample collector (15) is arranged on the actuation box section (3) such that when the actuation box section (3) is docked with the main box section (2) or is in the docking position, the sample collector is contained in the sample receiving cavity (17) in the main box section (2).

7. The processing box (1) according to claim 1 or 2, wherein, The main box section (2) is characterized by at least one guide rib (18) or recess, configured to interact with the corresponding recess (19) or guide rib in the actuation box section (3) during docking.

8. The processing box (1) according to claim 7, wherein, The at least one guide rib (18) of the main box section (2) is characterized by a tapered edge (20) in the corresponding recess (19) for insertion into the actuation box section (3).

9. The processing box (1) according to claim 1 or 2, wherein, The main box section (2) or the actuation box section (3) is characterized by a locking rib (21) having locking recesses (23a, 23b) arranged on opposite sides of the locking rib (21), and the corresponding actuation box section (3) or the main box section (2) is characterized by two opposing elastic hooks (24a, 24b), each elastic hook being configured to interact with the corresponding locking recesses (23a, 23b) on the locking rib, wherein the locking rib (21) has a tapered edge configured to be inserted between the elastic hooks during docking.

10. The processing box (1) according to claim 1 or 2, wherein, The container piercing element (6) includes a base portion (25), at least one side portion (26), a sharp portion (27) away from the base portion, and at least one recess (28) disposed in the side portion (26) and extending between the base portion (25) and the sharp portion (27).

11. The processing box (1) according to claim 1 or 2, wherein, The container piercing element and the container retaining element constitute the parts of a single unit (42).

12. The processing box (1) according to claim 11, wherein, The liquid storage chamber (4) includes a sidewall (37) and a base surface (43) characterized by a protrusion (22), and the individual unit is fixed in the liquid storage chamber (4) by arranging a portion of the individual unit between the protrusion and the sidewall.

13. The processing box (1) according to claim 1 or 2, wherein, The actuator box section (3) is connected to the main box section (2) via a pivot connection, wherein - The actuation box section (3) or the main box section (2) is characterized by comprising a first end (13) of two opposing clamping arms (30a, 30b) and an elastic arm (29) disposed between the clamping arms, each of the clamping arms being characterized by a pivoting protrusion (31a, 31b), and the pivoting protrusions being disposed opposite to each other; and - The corresponding main box section (2) or the actuation box section (3) is characterized by a connecting hub (32) for insertion between the two clamping arms (30a, 30b), the connecting hub (32) including a hole (33) for receiving the pivoting protrusion and having an outer periphery for interacting with the elastic arm (29); The first segment (S1) of the outer periphery has a shorter distance (R1) from the center line of the hole compared to the second segment (S2) of the outer periphery. The first segment and the second segment of the outer periphery are connected by a third segment (S3) of the outer periphery, which has an increased distance from the center line (C) of the hole. The third segment of the outer periphery is arranged such that the rotational movement of the actuating box segment relative to the main box segment when the elastic arm interacts with the third segment will face limited resistance.

14. The processing box (1) according to claim 1 or 2, wherein, The actuator box section (3) is connected to the main box section (2) via a pivot connection, wherein - The actuation box section (3) or the main box section (2) is characterized by a first end (13) comprising two opposing clamping arms (30a, 30b), each of the clamping arms being characterized by a pivoting protrusion (31a, 31b), and the pivoting protrusions being arranged opposite to each other; and - The corresponding main box section (2) or the actuating box section is characterized by a connecting hub (32) for insertion between the two clamping arms, the connecting hub (32) including a hole (33) for receiving the pivoting protrusion, the hole being defined by a middle section (34a) having a circular inner wall and a first section (34b) and a second section (34c) arranged on opposite sides of the middle section, each of the first section and the second section of the connecting hub (32) including a recess (35) extending between the hole (33) and the outer periphery of the connecting hub (32). - Each of the pivoting protrusions (31a, 31b) has an outer periphery of the protrusion, the outer periphery of the protrusion including two opposite first segments (K1) and two opposite second segments (K2) configured to mate with the circular inner wall, wherein the distance between each second segment (K2) of the outer periphery of the protrusion is shorter than the maximum distance between each first segment (K1) of the outer periphery of the protrusion, and the recess (35) of each of the first and second segments of the connecting hub (32) has a width smaller than the maximum distance between each first segment (K1) of the outer periphery of the protrusion and greater than the maximum distance between each second segment (K2) of the outer periphery of the protrusion.

15. A method for introducing a sample into a processing box, the processing box (1) comprising: - Main box section (2), and -Actuator box section (3). The main box section (2) includes: -Liquid storage chamber (4). -Liquid container (5) - Container piercing element (6), and - Flexible container holding element (9). The actuation box section (3) is configured to dock with the main box section (2) and includes an actuation member (10) configured to interact with the liquid container (5) during docking. The liquid container (5), the container piercing element (6) and the container holding element (9) are arranged in the liquid storage chamber (4), the liquid container being filled with a suitable processing liquid and characterized by a piercing dispensing side (16), and the container piercing element facing the dispensing side; The liquid container (5) is held in a first position by the container holding element (9), in which the dispensing side is separated from the container piercing element; The container holding element includes a resilient arm that contacts the edge of the liquid container (5), the resilient arm being arranged to deflect away from the edge when the actuating member moves the liquid container to a second position. The method includes the following steps: - Keep the actuator box section in the non-dock position; - Collect samples using the sample collector (15); and - The actuation box section (3) is pivoted to the docking position, in which the actuation member (10) has moved the liquid container from the first position to the second position during the docking of the actuation box section, wherein the elastic arm deflects away from the edge and the dispensing side (16) is pierced by the container piercing element.

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