Anti-wrinkle composition and preparation method thereof, skin care product and preparation method thereof

Abstract

The present invention relates to the technical field of daily skin care products, and in particular, to an anti-wrinkle composition and a preparation method thereof, and a skin care product and a preparation method thereof. The raw materials of the anti-wrinkle composition include a hydrolyzed red algae extract, a bifid yeast fermentation product lysate, and glycogen, wherein the mass ratio of the hydrolyzed red algae extract, the bifid yeast fermentation product lysate, and the glycogen is (5-15): (2.5-10): (1-5). The anti-wrinkle composition has excellent anti-wrinkle effects and is mild, is not prone to irritation to the skin, and does not burden the skin. At the same time, the anti-wrinkle composition is abundant in sources and has low cost.

Description

Technical Field

[0001] The present invention relates to the technical field of daily skin care products, in particular to an anti-wrinkle composition and a preparation method thereof, and a skin care product and a preparation method thereof. Background Art

[0002] With the increasing prevalence of an aging global population, anti-aging and anti-wrinkle treatments have become a hot topic. Wrinkles can be caused by many factors, but at a microscopic level, the dermis is most closely linked to wrinkle formation. The dermis is the skin's supporting tissue, and its cells, known as fibroblasts, are primarily responsible for the production of collagen and elastin fibers. Collagen fibers form a dense network to maintain skin organization and resistance, while finer elastin fibers provide suppleness and elasticity. The number of collagen and elastin fibers decreases with aging, leading to wrinkles. Therefore, in vitro evaluation of anti-wrinkle efficacy generally uses the amount of collagen production promoted as a criterion; in vivo evaluation of anti-wrinkle efficacy generally uses the degree of improvement in wrinkle depth and area as criteria.

[0003] Currently, most cosmetic anti-wrinkle ingredients on the market are derived from chemical synthesis, including retinol, phosphatidylcholine, collagen, and peptides. Asians have a low tolerance for retinol, which can cause skin irritation, including peeling, redness, and sometimes local stinging. phosphatidylcholine stimulates the production and construction of aminoglucans and proteoglycans, which build the skin's support network and plump it up. However, these effects are generally only apparent at high dosages. For example, L'Oréal's eye cream contains a high 20% phosphatidylcholine content. Long-term, excessive use can burden the skin and may cause allergies, redness, stinging, and itching. Collagen is generally derived from animal sources, which is controversial. Its high molecular weight makes it difficult to absorb through the skin and has low bioavailability. Therefore, there is a need for an anti-wrinkle substance that is less irritating, easily absorbed, widely available, and has excellent anti-wrinkle effects.

[0004] In view of this, the present invention is proposed. Summary of the Invention

[0005] The present invention aims to provide an anti-wrinkle composition and a preparation method thereof, and a skin care product and a preparation method thereof. The anti-wrinkle composition provided in the embodiments of the present invention has excellent anti-wrinkle effects, is less irritating to the skin, and is not prone to causing skin burden.

[0006] The present invention is achieved in that:

[0007] In a first aspect, the present invention provides an anti-wrinkle composition, the raw materials of which include hydrolyzed red algae extract, bifid yeast fermentation product lysate and glycogen, wherein the mass ratio of the hydrolyzed red algae extract, the bifid yeast fermentation product lysate and the glycogen is (5-15): (2.5-10): (1-5).

[0008] In an optional embodiment, the active ingredients are composed of the hydrolyzed red algae extract, the bifid yeast fermentation product lysate and the glycogen, wherein the mass ratio of the hydrolyzed red algae extract, the bifid yeast fermentation product lysate and the glycogen is (5-15): (2.5-10): (1-5).

[0009] In an optional embodiment, the mass ratio of the hydrolyzed red algae extract, the bifid yeast fermentation product lysate and the glycogen is (5-15): (2.5-5): (3-5).

[0010] In an optional embodiment, the mass ratio of the hydrolyzed red algae extract, the bifid yeast fermentation product lysate, and the glycogen is 15:2.5:5.

[0011] In a second aspect, the present invention provides a method for preparing the anti-wrinkle composition according to the aforementioned embodiment, comprising: mixing a red algae extract, a lysate of a bifid yeast fermentation product, and glycogen.

[0012] In a third aspect, the present invention provides a skin care product comprising the anti-wrinkle composition according to any one of the aforementioned embodiments, wherein the mass content of the anti-wrinkle composition in the skin care product is 1-10%.

[0013] In an optional embodiment, the anti-wrinkle composition is contained in the skin care product in an amount of 3-5% by weight.

[0014] In an optional embodiment, the skin care product includes any one of skin care lotion, essence, lotion, facial ointment, facial cream and facial mask.

[0015] In an optional embodiment, the skin care product further comprises a matrix;

[0016] Preferably, the matrix comprises at least one of a wetting agent, a penetration enhancer, a thickening stabilizer, a bactericidal preservative, an emulsifier and a surfactant.

[0017] In a fourth aspect, the present invention provides a method for preparing a skin care product, comprising: mixing the anti-wrinkle composition described in any one of the aforementioned embodiments.

[0018] The present invention has the following beneficial effects: By specifically selecting and mixing hydrolyzed red algae extract, bifid yeast fermentation product lysate, and glycogen in a specific ratio, the present invention provides an anti-wrinkle composition comprising these three ingredients, resulting in excellent anti-wrinkle effects. Furthermore, the resulting anti-wrinkle composition is mild, less likely to cause irritation, and less burdensome to the skin. Furthermore, the anti-wrinkle composition is readily available from abundant sources and is relatively low in cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for use in the embodiments. It should be understood that the following drawings only illustrate certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying any creative work.

[0020] Figure 1 The relative expression level of eln1 in zebrafish tested for the anti-wrinkle composition of Example 3 provided in Example 3;

[0021] Figure 2 The relative expression level of eln2 in zebrafish tested for the anti-wrinkle composition of Example 3 provided in Example 3;

[0022] Figure 3 The anti-wrinkle efficacy of the anti-wrinkle composition of Example 3 provided in Test Example 4 was measured on the zebrafish tail fin area. DETAILED DESCRIPTION

[0023] To make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention are described clearly and completely below. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer are used. Where the manufacturer of the reagents or instruments is not specified, all are conventional products that can be purchased commercially.

[0024] In a first aspect, the present invention provides an anti-wrinkle composition, the raw materials of which include hydrolyzed red algae extract, bifid yeast fermentation product lysate and glycogen, wherein the mass ratio of the hydrolyzed red algae extract, the bifid yeast fermentation product lysate and the glycogen is (5-15): (2.5-10): (1-5).

[0025] The embodiment of the present invention uses a hydrolyzed red algae extract as one of the active ingredients of the anti-wrinkle combination. Compared with the current anti-wrinkle compositions of similar plant origin, although the hydrolyzed red algae extract used in the anti-wrinkle composition is generally used as a skin conditioner and is not the main anti-wrinkle ingredient, the anti-wrinkle composition formed has excellent anti-wrinkle effects. Other plant anti-wrinkle compositions use a relatively high dosage, which leads to a greater risk of discoloration of the formula, and the high ionicity of the plant extract is not conducive to the application of the formula. The anti-wrinkle composition formed by the hydrolyzed red algae extract used in the embodiment of the present invention has a smaller dosage and a good anti-wrinkle effect. At the same time, compared with the plant ingredients on the market, the hydrolyzed red algae extract is an older algae species and reproduces quickly. Since it has different metabolic pathways and metabolites from terrestrial plants, the active components of algae are of many types and high activity, which is more conducive to improving the effect of the anti-wrinkle composition.

[0026] It should be noted that the hydrolyzed red algae extract, bifid yeast fermentation product lysate, and glycogen used in the embodiments of the present invention are all raw materials that can be purchased according to the prior art. For example, the hydrolyzed red algae extract was purchased from SEPPIC / BASF / Chemyunion / CLR Chemisches Laboratorium Dr.Kurt Richter GmbH; the bifid yeast fermentation product lysate was purchased from Natural Solution / CLR Chemisches Laboratorium Dr.Kurt RichterGmbH / Uniproma / Guangdong Perfect Life Science and Health Technology Research Institute Co., Ltd.; and the glycogen was purchased from Mibelle AGBiochemistry / Glico Nutrition.

[0027] Furthermore, the active ingredients forming the anti-wrinkle composition are composed of the hydrolyzed red algae extract, the bifid yeast fermentation product lysate and the glycogen, and the ratio of the three is (5-15): (2.5-10): (1-5).

[0028] In an embodiment of the present invention with better effects, the mass ratio of the hydrolyzed red algae extract, the bifid yeast fermentation product lysate, and the glycogen is (5-15):(2.5-5):(3-5). The optimal ratio is 15:2.5:5.

[0029] In a second aspect, the present invention provides a method for preparing the anti-wrinkle composition described in the aforementioned embodiment, comprising: directly mixing the above three to obtain the desired anti-wrinkle composition.

[0030] In a third aspect, an embodiment of the present invention provides a skin care product, comprising the anti-wrinkle composition according to any one of the preceding embodiments, wherein the mass content of the anti-wrinkle composition in the skin care product is 1-10%, for example, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9% and 10%, or any value between 1-10%, preferably 3-5%.

[0031] Furthermore, the dosage form of the above-mentioned skin care product can be selected from one of the skin care product dosage forms known in the prior art, such as skin care water, makeup remover water, makeup remover milk, makeup remover cream, essence, lotion, facial cream, facial cream and facial mask.

[0032] Furthermore, the skin care product also includes a matrix; the matrix can be an excipient, or other ingredients with skin care benefits that do not affect the anti-wrinkle effect of the anti-wrinkle composition, or both. The excipients used are known excipients, or appropriate excipients can be selected based on the actual dosage form of the skin care product. For example, the matrix includes at least one of a wetting agent, a penetration enhancer, a thickening stabilizer, a bactericidal preservative, an emulsifier, and a surfactant. Specifically, for example, wetting agents include but are not limited to isopropyl myristate, penetration enhancers include but are not limited to azone, thickening stabilizers include but are not limited to xanthan gum and xanthan gum, bactericidal preservatives include but are not limited to methylparaben and ethylparaben, and emulsifiers include but are not limited to glyceryl stearate. Surfactants include but are not limited to potassium cetyl phosphate and cetearyl glucoside.

[0033] The features and performance of the present invention are further described in detail below with reference to the embodiments.

[0034] Example 1

[0035] This embodiment of the present invention provides an anti-wrinkle composition, the active ingredients of which are composed of a hydrolyzed red algae extract, a bifid yeast fermentation lysate, and glycogen. The mass ratio of the hydrolyzed red algae extract, the bifid yeast fermentation lysate, and the glycogen is 5:2.5:1. The hydrolyzed red algae extract was purchased from BASF; the bifid yeast fermentation lysate was purchased from Guangdong Perfect Life Science and Health Technology Research Institute Co., Ltd.; and the glycogen was purchased from Mibelle AG Biochemistry.

[0036] Example 2

[0037] An embodiment of the present invention provides an anti-wrinkle composition, the active ingredients of which are composed of a hydrolyzed red algae extract, a bifid yeast fermentation product lysate, and glycogen, wherein the mass ratio of the hydrolyzed red algae extract, the bifid yeast fermentation product lysate, and the glycogen is 10:5:3.

[0038] Example 3

[0039] An embodiment of the present invention provides an anti-wrinkle composition, the active ingredients of which are composed of a hydrolyzed red algae extract, a bifid yeast fermentation product lysate, and glycogen, wherein the mass ratio of the hydrolyzed red algae extract, the bifid yeast fermentation product lysate, and the glycogen is 15:2.5:5.

[0040] Example 4

[0041] An embodiment of the present invention provides an anti-wrinkle composition, the active ingredients of which are composed of a hydrolyzed red algae extract, a bifid yeast fermentation product lysate, and glycogen, wherein the mass ratio of the hydrolyzed red algae extract, the bifid yeast fermentation product lysate, and the glycogen is 15:10:1.

[0042] Comparative Example 1: The comparative example provides an anti-wrinkle composition, wherein the active ingredients are composed of a hydrolyzed red algae extract and a bifid yeast fermentation product lysate, wherein the mass ratio of the hydrolyzed red algae extract to the bifid yeast fermentation product lysate is 15:2.5.

[0043] Comparative Example 2: The comparative example provides an anti-wrinkle composition, wherein the active ingredients of the composition are composed of a lysate of a bifid yeast fermentation product and glycogen, wherein the mass ratio of the lysate of a bifid yeast fermentation product to the glycogen is 2.5:5.

[0044] Comparative Example 3: The comparative example provides an anti-wrinkle composition, wherein the active ingredients thereof consist of a hydrolyzed red algae extract and glycogen, wherein the mass ratio of the hydrolyzed red algae extract to the glycogen is 15:5.

[0045] Comparative Example 4: The comparative example provides an anti-wrinkle composition, the active ingredient of which is only the hydrolyzed red algae extract.

[0046] Comparative Example 5: The comparative example provides an anti-wrinkle composition, the active ingredient of which is only a lysate of a bifid yeast fermentation product.

[0047] Comparative Example 6: The comparative example provides an anti-wrinkle composition, the active ingredient of which is only glycogen.

[0048] Comparative Example 7: This comparative example provides an anti-wrinkle composition, wherein the active ingredients are composed of an algae extract, a bifid yeast fermentation product lysate, and glycogen, wherein the mass ratio of the algae extract, the bifid yeast fermentation product lysate, and the glycogen is 15:2.5:5. The algae extract was purchased from Biocogent.

[0049] Comparative Example 8: The comparative example provides an anti-wrinkle composition, wherein the active ingredients are composed of hydrolyzed red algae extract, yeast extract, and glycogen, wherein the mass ratio of the hydrolyzed red algae extract, yeast extract, and glycogen is 15:2.5:5. The yeast extract is purchased from Lucas Meyer Cosmetics.

[0050] Comparative Example 9: This comparative example provides an anti-wrinkle composition, the active ingredients of which are composed of a hydrolyzed red algae extract, a bifid yeast fermentation product lysate, and oligofructose. The mass ratio of the hydrolyzed red algae extract, the bifid yeast fermentation product lysate, and the oligofructose is 15:2.5:5. The oligofructose was purchased from Quantum Hi-Tech (Guangdong) Biological Co., Ltd.

[0051] Test Example 1

[0052] Regarding aging, researchers have proposed many theories of aging, among which the reactive oxygen free radical theory is the most supported theory. The mechanism by which free radicals cause skin aging is complex, but the phenomena and consequences caused are very simple: aging-related changes in some skin structures and functions, such as decreased skin elasticity, collagen degeneration, wrinkles, sagging, etc., are all closely related to the damaging effects of free radicals. Antioxidants can eliminate these excess free radicals or indirectly consume substances that easily generate free radicals, thereby playing a certain anti-aging role. In addition, elastin is present in the extracellular matrix. Although it accounts for less than 2% of dermal proteins, it plays an important role in maintaining skin elasticity and is closely related to the appearance of skin wrinkles. Elastase inhibition can indirectly reflect the anti-wrinkle effect of the composition.

[0053] Therefore, the embodiment of the present invention adopts the DPPH method to detect the antioxidant capacity of the composition. Specifically, the DPPH free radical scavenging rate test process is as follows:

[0054] The anti-wrinkle composition of the embodiment or comparative example was diluted with deionized water to a concentration of 25% (the mass concentration of the anti-wrinkle composition of the embodiment or comparative example was 25%). 100 μL of the sample solution was added to a 96-well plate, and 100 μL of a 0.2 mmol / L DPPH anhydrous ethanol solution was added. After shaking evenly, the mixture was placed in a dark place at 25°C to react for 30 minutes. The absorbance A1 of the mixture was then measured at a wavelength of 517 nm. The same procedure was performed using 100 μL of anhydrous ethanol instead of the DPPH anhydrous ethanol solution. The same procedure was performed using 100 μL of anhydrous ethanol instead of the sample. Vitamin C solution was used as a positive control. Three parallel experiments were performed for each group, and the average value was calculated. The free radical scavenging ability of the anti-aging repair compositions of different groups was calculated according to the DPPH scavenging rate formula.

[0055] DPPH clearance rate = [1-(A1-A2) / A3] × 100%

[0056] Where:

[0057] A1 is the absorbance value of 100 μL DPPH working solution + 100 μL sample;

[0058] A2 is the absorbance value of 100 μL anhydrous ethanol + 100 μL sample;

[0059] A3 is the absorbance value of 100 μL DPPH working solution + 100 μL anhydrous ethanol.

[0060] See Table 1 for the results.

[0061] Table 1 Test results of DPPH free radical scavenging rate

[0062]

[0063]

[0064] As can be seen in Table 1, the compositions obtained in Examples 1-4 all exhibited excellent DPPH free radical scavenging capabilities, with the highest scavenging rate reaching 77.79%. The free radical scavenging effectiveness of the compositions obtained in Examples 1-4 was significantly greater than that of the individual components used in Comparative Examples 4-6, and also significantly greater than that of any two components combined in Comparative Examples 1-3. The compositions obtained in Comparative Examples 7-9, due to the substitution of a component with a component having similar efficacy in the compositions of the present invention, also exhibited significantly lower free radical scavenging effectiveness than the samples in Examples 1-4. This further demonstrates that the components of the anti-wrinkle composition of the present invention exhibit synergistic effects, and that all three components are essential, resulting in the compositions of the present invention exhibiting strong free radical scavenging capabilities.

[0065] Test Example 2

[0066] Elastase activity inhibition test

[0067] Take the anti-wrinkle composition prepared in the examples or comparative examples, dilute it with deionized water to a concentration of 5%; (the mass concentration of the anti-wrinkle composition in the examples or comparative examples is 5%), add 1 mL of the sample solution and 1 mL of the elastase solution to a 5 mL centrifuge tube, mix thoroughly and react at 37°C for 5 minutes, then add 1 mL of the substrate solution and continue to react at 37°C for 30 minutes. After incubation, centrifuge at 1000 rpm for 5 minutes, draw 200 μL of the supernatant into a 96-well plate, and detect the absorbance at a wavelength of 405 nm using a microplate reader. At the same time, set up a blank substrate group (an equal volume of 10% DMSO instead of substrate), a 100% enzyme activity control group, and a blank control group. Three parallel experiments were performed for each group, with sivelestat sodium as a positive control. The calculation formula for the elastase inhibition rate is as follows:

[0068] Elastase inhibition rate = [1-(Y1-Y2) / (Y3-Y4)] × 100%

[0069] Where:

[0070] Y1 is the absorbance value of the test sample + elastase solution + substrate solution;

[0071] Y2 is the absorbance value of the test sample + elastase solution + 10% DMSO;

[0072] Y3 is the absorbance value of PBS buffer solution + elastase solution + substrate solution;

[0073] Y4 is the absorbance value of PBS buffer solution + elastase solution + 10% DMSO.

[0074] See Table 2 for the results.

[0075] Table 2 Test results of elastase activity inhibition

[0076]

[0077] As can be seen from Table 2, the compositions obtained in Examples 1-4 all exhibited good elastase activity inhibition, with the highest clearance rate reaching 86.53%. The elastase activity inhibition abilities of the compositions obtained in Examples 1-4 were significantly stronger than those of the individual components used in Comparative Examples 4-6, and were also significantly stronger than the effects of any two components combined in Comparative Examples 1-3. The compositions obtained in Comparative Examples 7-9, due to the replacement of a component in the compositions of the present invention with a component having similar efficacy, also exhibited significantly lower elastase activity inhibition abilities than the samples in Examples 1-4. This further demonstrates that the components in the anti-wrinkle composition of the present invention exhibit synergistic effects, and that all three components are essential, resulting in the compositions of the present invention exhibiting strong elastase activity inhibition abilities.

[0078] Test Example 3

[0079] Zebrafish firming efficacy test

[0080] AB wild-type zebrafish embryos at 4 dpf (96 hpf) post-fertilization were selected and randomly distributed into 6-well plates, with 30 per well. They were divided into a normal control group, a positive group, and a test substance group. Three concentrations of the test substance were set for each group (the sample was diluted with embryo culture medium to 0.1%, 0.05%, and 0.025% solution), and three replicate wells were set for each group. Without harming the fry, the buffer water in the 6-well plate was removed and 3 mL of the corresponding solution was quickly added to the well plate. The culture panel was covered and incubated in a biochemical incubator at 28.5°C in the dark for 24 hours. The normal control group contained zebrafish embryos and embryo culture medium; the positive control group contained zebrafish embryos and a 20 mg / mL L-carnosine (CAS No. 305-84-0, 99% purity) solution prepared in embryo culture medium; and the test group used the anti-wrinkle composition of Example 3 containing the test substance and zebrafish embryos. The test substance was divided into three different concentration groups according to the MTC as needed.

[0081] Total RNA from zebrafish in each group was extracted using an RNA rapid extraction kit, and cDNA was synthesized. Fluorescence quantitative PCR was used to detect the internal reference gene and the target genes eln1 and eln2 in each group, and the Ct values ​​of qPCR detection were recorded. The relative expression levels of genes in each group of samples were calculated according to the following formula.

[0082] Relative RNA expression = 2^ -ΔΔct ;

[0083] ΔΔC(t) = ΔC(t) target gene - ΔC(t) control group average;

[0084] ΔC(t) = C(t) target gene - C(t) internal reference gene.

[0085] The mean value (Mean) and standard error (Standard Error, SE) of each group of experiments were calculated. The statistical results were expressed as Mean ± SE. SPSS software was used to perform variance analysis on the data. The normal control group (or solvent control group) (i.e. Figure 1 and Figure 2 The CONTROL group in the experiment was used as the standard, and Excel was used to draw the graph. P < 0.05 indicated a significant difference.

[0086] Results see Figure 1 and Figure 2 According to the results, compared with the control group, the relative expression levels of eln1 and eln2 genes in the positive group zebrafish were extremely significantly increased (P<0.001), indicating that the positive drug (L-carnosine) has a firming effect; the multi-pathway anti-wrinkle patented composition had an extremely significant increase in the expression levels of eln1 and eln2 genes in zebrafish at concentrations of 0.1%, 0.05%, and 0.025% (P<0.001), indicating that it has a significant firming effect.

[0087] Note: Figure 1 and Figure 2 Note: “*” indicates significant level (P<0.05), “**” indicates relatively significant level (P<0.01), and “***” indicates extremely significant level (P<0.001).

[0088] Test Example 4

[0089] Zebrafish anti-wrinkle efficacy test

[0090] Normally developed zebrafish larvae at 2 days post-fertilization (dpf) were selected and placed in six-well cell culture plates, with 15 larvae per well. These larvae were divided into a normal control group, a model group, a positive control group, and a test substance test group. Three test substance concentrations were set (samples were diluted with embryo culture medium to 0.1%, 0.05%, and 0.025% solutions). Without harming the larvae, the standard dilution water in the six-well plate was removed, and 3 mL of the corresponding test substance dilution solution was quickly added to each well.

[0091] Wrap the six-well plate with aluminum foil and incubate in a biochemical incubator (28.5℃±1.0℃) in the dark for 2h. 2 Under the conditions of 30 minutes, the UV irradiation was carried out three times, with each irradiation time of 15 minutes and an interval of 30 minutes. The cells were incubated in a biochemical incubator at (28.5℃±0.5℃) in the dark for 22 hours (3 dpf) and then observed. The death of zebrafish and other toxic effects were recorded. Among them, the normal control group contained zebrafish embryos and embryo culture medium, and no model was established; the model group contained zebrafish embryos and embryo culture medium, and was subjected to UV irradiation modeling; the positive control group contained zebrafish embryos and a 200μg / mL dibenzophenone-4 (CAS No.: 4065-45-6, purity 99%) solution prepared in embryo culture medium, and was subjected to UV irradiation modeling; the test substance test group: contained the test substance and zebrafish embryos. The test substance was set up in two different concentration groups according to MTC as needed, and UV modeling was performed.

[0092] After incubation, randomly select at least 12 zebrafish with normal phenotype and behavior, fix them with 3% methylcellulose, and observe and photograph them under a stereomicroscope. When photographing, the zebrafish should face left, side down, and body horizontal. All zebrafish photographs must be completed under the same instrument and environmental conditions, and the body position of the zebrafish should be consistent.

[0093] After taking the photos, use image analysis software to analyze the zebrafish images. The caudal fin is the selected quantitative region. Set the analysis parameter to area and analyze the images to obtain data. Ten valid data points are collected for each group.

[0094] The tail fin area was recorded as A, and the mean (Mean) and standard error (SE) of each group were calculated. Statistical results were expressed as Mean ± SE. Data were analyzed by variance analysis using SPSS software. The tail fin area of ​​each experimental group was compared using the model control group (or solvent control group) as the standard. P < 0.05 indicated a significant difference.

[0095] According to the average value A of the tail fin area of ​​each experimental group, the anti-wrinkle efficacy of the test substance in each concentration group was calculated using the following formula:

[0096] Anti-wrinkle efficacy (%) = (A(sample group) - A(model control group)) / (A(blank control group) - A(model control group)) x 100%.

[0097] Structure Figure 3 .according to Figure 3 As shown, compared with the blank group, the caudal fin area of ​​the zebrafish in the model group was significantly reduced (P < 0.001), indicating successful modeling. Furthermore, compared with the model group, the caudal fin area of ​​the zebrafish in the positive control group was significantly increased after administration (P < 0.001), indicating that the positive drug (benzophenone) has anti-wrinkle efficacy. At concentrations of 0.1%, 0.05%, and 0.025%, the composition of Example 3 significantly increased the caudal fin area of ​​the zebrafish compared with the model group (P < 0.05), demonstrating anti-wrinkle efficacy. Furthermore, at a concentration of 0.025%, the zebrafish caudal fin wrinkle recovery rate was the highest, at 62.27%, demonstrating a strong anti-wrinkle effect.

[0098] Note: Figure 3 Note: Compared with the blank group, “#” indicates P﹤0.05, “##” indicates P﹤0.01, and “###” indicates P﹤0.001; compared with the model group, “*” indicates P﹤0.05, “**” indicates P﹤0.01, and “***” indicates P﹤0.001.

[0099] The foregoing description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art will readily appreciate that various modifications and variations of the present invention are possible. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention are intended to be within the scope of protection of the present invention.

Claims

1. An anti-wrinkle composition, characterized in that The raw materials include hydrolyzed red algae extract, bifid yeast fermentation product lysate and glycogen, wherein the mass ratio of the hydrolyzed red algae extract, the bifid yeast fermentation product lysate and the glycogen is (5-15): (2.5-10): (1-5).

2. The anti-wrinkle composition according to claim 1, characterized in that The active ingredients are composed of the hydrolyzed red algae extract, the bifid yeast fermentation product lysate and the glycogen, wherein the mass ratio of the hydrolyzed red algae extract, the bifid yeast fermentation product lysate and the glycogen is (5-15): (2.5-10): (1-5).

3. The anti-wrinkle composition according to claim 1 or 2, characterized in that The mass ratio of the hydrolyzed red algae extract, the bifid yeast fermentation product lysate and the glycogen is (5-15): (2.5-5): (3-5).

4. The anti-wrinkle composition according to claim 1 or 2, characterized in that The mass ratio of the hydrolyzed red algae extract, the bifid yeast fermentation product lysate and the glycogen is 15:2.5:

5.

5. A method for preparing the anti-wrinkle composition according to claim 1, characterized in that: include: The hydrolyzed red algae extract, bifid yeast fermentation product lysate and glycogen are mixed.

6. A skin care product, characterized in that: The anti-wrinkle composition comprises the anti-wrinkle composition according to any one of claims 1 to 4, wherein the mass content of the anti-wrinkle composition in the skin care product is 1-10%.

7. The skin care product according to claim 6, characterized in that The anti-wrinkle composition is contained in the skin care product in an amount of 3-5% by weight.

8. The skin care product according to claim 6, characterized in that The skin care products include any one of skin care lotion, essence, emulsion, facial ointment, facial cream and facial mask.

9. The skin care product according to claim 6, characterized in that The skin care product further comprises a base.

10. The skin care product according to claim 9, characterized in that The matrix includes at least one of a wetting agent, a penetration enhancer, a thickening stabilizer, a bactericidal preservative, an emulsifier and a surfactant.

11. A method for preparing a skin care product, characterized in that: include: The anti-wrinkle composition according to any one of claims 1 to 4 is mixed.

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