Application of plant-derived active substance stepholidine in preventing and controlling weeds

Abstract

The application belongs to the technical field of field weed control, and particularly relates to application of a plant-derived active substance, stepholidine, in weed control. The application research finds that stepholidine can effectively inhibit the growth of the sprout length and root length of barnyard grass, cyperus difformis and bidens pilosa, and the inhibitory effect on the sprout length and root length of barnyard grass is most obvious. Compared with barnyard grass, the inhibitory effect of stepholidine on the sprout length and root length of cyperus difformis and bidens pilosa is slightly weaker, but through comparison, it is found that the inhibitory effect of high-concentration or low-concentration stepholidine on the sprout length and root length of cyperus difformis is better than that of herbicide propachlor under the same conditions, and stepholidine is more green and efficient.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of field weed control, and specifically discloses application of a plant-derived active substance, stepholidine, in weed control. BACKGROUND

[0002] There are about 580 species of common weeds in Chinese farmland, of which 129 are weeds in paddy fields. Weeds can cause the yield and quality of crops to decrease, and thus must be controlled. At present, commercial herbicides are mainly chemical herbicides, such as dichloroacetamides, carboxylic acid derivatives, oxime ethers, thiazole carboxylic acids, triazoles, and dihydro-pyrazole dicarboxylic acids. However, the long-term and large-scale use of chemical herbicides has led to the rapid development of herbicide resistance in weeds, such as the occurrence of resistance in barnyard grass and Euphorbia humifusa, which poses a threat to rice yield.

[0003] Plant-derived herbicides are active substances with herbicidal effects extracted from plants. At present, more than 100 natural compounds with herbicidal activity have been found in the world. Therefore, one of the effective ways to solve the environmental compatibility problem is to widely screen natural products with herbicidal activity in plants, directly develop biological herbicides, or perform structural derivation and optimization. Stepholidine is an active chemical component of Stephania sinica Diels, which has various therapeutic effects on the human body, but its application in field weed control is rarely reported. SUMMARY

[0004] In view of the deficiencies of the prior art, the present application mainly studies the application of a plant-derived active substance, stepholidine, in weed control.

[0005] The present application first provides a new use of stepholidine in weed control.

[0006] Preferably, the weeds are grasses, sedges, and broadleaf weeds.

[0007] Preferably, the grasses are barnyard grass, the sedges are altemifolia, and the broadleaf weeds are bidens pilosa; more preferably, the grasses are barnyard grass.

[0008] The present application also provides a herbicide, the components of which include stepholidine, and the stepholidine is usually formulated into a herbicide component in various ways using formulation aids such as solvents, carriers, and the like.

[0009] The herbicide mainly acts on field weeds, and preferably, the weeds include grasses, sedges, and broadleaf weeds.

[0010] Preferably, the weeds are grasses barnyard grass, sedges altemifolia, and broadleaf weeds bidens pilosa; more preferably, the grasses are barnyard grass.

[0011] Preferably, the concentration of the stepholidine is 0.00019531 g / L to 0.05 g / L.

[0012] The herbicide can be prepared into various formulation forms, such as powder, gel, water dispersible granule, water dispersible tablet, wettable powder, effervescent compressed tablet, emulsion, microemulsifiable concentrate, aqueous emulsion, flowable oil, water dispersant, oil dispersant, suspoemulsion, microcapsule suspending agent, emulsion granule, soluble liquid, etc.

[0013] Compared with the prior art, the excellent effects of the present application mainly lie in the following aspects:

[0014] The present application provides a new use of the plant-derived active substance stepholidine, which can effectively prevent and control weeds. The stepholidine has inhibitory effects on the bud length and root length of weeds such as barnyard grass, ischaemum rugosum and bidens pilosa, and the inhibitory effect on barnyard grass is the strongest. The present application provides a new technical solution for green treatment of weeds and realization of sustainable agricultural development. DETAILED DESCRIPTION

[0015] The technical solutions of the present application will be clearly and completely described below in combination with the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the present application.

[0016] In the embodiments of the present application, the preparation is carried out according to the conventional conditions or the conditions suggested by the manufacturer, and the reagents or instruments not marked with the manufacturer are all conventional products that can be commercially purchased.

[0017] 1. Test materials and methods

[0018] 1.1 Test agent

[0019] Stepholidine, propachlor and acetic acid.

[0020] 1.2 Test weeds

[0021] Barnyard grass, ischaemum rugosum and bidens pilosa.

[0022] 1.3 Test method

[0023] Pre-emergence treatment of weeds by culture dish method

[0024] The seeds of three weeds, Echinochloa crus-galli, Cyperus difformis and Bidens pilosa, were evenly spread on moist filter paper and germinated at 28°C. 20 seeds of each weed with uniform germination were selected and evenly spread on a culture dish. 2.5 mg of stepholidine was dissolved in 50 mL of 0.025% acetic acid water to form a high concentration of 0.05 g / L. The high concentration was diluted with 0.025% acetic acid water to form five concentrations of 0.025, 0.0125, 0.003125, 0.00078125 and 0.00019531. 3 mL of the treatment was used to soak the seeds of the three weeds. After the treatment, the seeds of Echinochloa crus-galli, Cyperus difformis and Bidens pilosa were placed in an artificial climate chamber (14 h of light at 28°C and 80% RH, 10 h of darkness at 25°C and 75% RH). Each treatment was repeated three times. After 7 days of treatment, the inhibition rates of the herbicide on the radicle length and root length of the three weeds were calculated.

[0025] 2. Results analysis

[0026] 2.1 Inhibition of stepholidine on the radicle length of the three weeds

[0027] The inhibition rates of stepholidine on the radicle length of Echinochloa crus-galli, Cyperus difformis and Bidens pilosa are shown in Table 1.

[0028] Table 1

[0029]

[0030]

[0031] Note: Different letters in the same column indicate significant differences (Tukey-test, p<0.05).

[0032] The results showed that the inhibition effect of stepholidine on the radicle length of Echinochloa crus-galli was comparable to that of propachlor, the inhibition effect of 0.05 g / L stepholidine on Echinochloa crus-galli reached 90%, which was not significantly different from that of 0.05 g / L propachlor, and the inhibition effect of 0.00019531 g / L stepholidine on Echinochloa crus-galli still reached 70%, which was not significantly different from that of propachlor at the same concentration. The inhibition effect of stepholidine on the radicle length of Cyperus difformis and Bidens pilosa was weaker than that on Echinochloa crus-galli, the inhibition effect of 0.05 g / L stepholidine on Cyperus difformis reached 75%, which was better than that of 0.05 g / L propachlor (63%), and the inhibition effect of 0.00019531 g / L stepholidine on Cyperus difformis was comparable to that of 0.025 g / L propachlor. The inhibition effect of 0.05 g / L stepholidine on Bidens pilosa reached 54%, which was comparable to that of propachlor.

[0033] 2.2 Inhibition of stepholidine on the root length of the three weeds

[0034] The root length inhibition rates of Stephania tetrandra S. Moore on Echinochloa crus-galli, Cyperus difformis, Bidens pilosa were shown in Table 2.

[0035] Table 2

[0036]

[0037]

[0038] Note: Different letters in the same column indicate significant difference (Tukey-test, p<0.05).

[0039] The results showed that the inhibitory effect of Stephania tetrandra S. Moore on the root length of Echinochloa crus-galli was better than that of the control agent propanil, the inhibitory effect of 0.05 g / L Stephania tetrandra S. Moore on Echinochloa crus-galli reached 89%, which had no significant difference with that of 0.05 g / L propanil; but the inhibitory effect of low concentration 0.00019531 g / L Stephania tetrandra S. Moore on Echinochloa crus-galli still reached 75%, which was significantly better than that of 0.003125 g / L propanil. Compared with Echinochloa crus-galli, the inhibitory effect of Stephania tetrandra S. Moore on the root length of Cyperus difformis and Bidens pilosa was relatively weak, the inhibitory effect of 0.05 g / L Stephania tetrandra S. Moore on the root length of Cyperus difformis was 69%, which was significantly better than that of 0.05 g / L propanil, and the inhibitory effect of low concentration 0.00019531 g / L on the root length of Cyperus difformis was equivalent to that of 0.025 g / L propanil on the root length of Cyperus difformis; the inhibitory effect of Stephania tetrandra S. Moore on the root length of Bidens pilosa was equivalent to that of propanil, the inhibitory effect of 0.05 g / L Stephania tetrandra S. Moore on Bidens pilosa was 58%, which had no significant difference with that of 0.05 g / L propanil.

[0040] The embodiments of the present application are described in detail above, but the present application is not limited to the described embodiments. For those skilled in the art, various changes, modifications, replacements and variations of the embodiments are made without departing from the principles and spirits of the present application, which still fall within the protection scope of the present application.

Claims

1. The use of stepholidine in the control of weeds.

2. Use according to claim 1, characterized in that, The weeds are grasses, sedges and broadleaf weeds.

3. Use according to claim 2, characterized in that, The weeds are Echinochloa crus-galli, Cyperus difformis and Bidens pilosa.

4. Use of stepholidine in the production of a herbicide for controlling weeds, characterized in that, The components of the herbicide include stepholidine and a carrier acceptable for processing of a pesticide dosage form.

5. Use according to claim 4, characterized in that, The weeds include grasses, sedges and broadleaf weeds.

6. Use according to claim 5, characterized in that, The weeds are Echinochloa crus-galli, Cyperus difformis and Bidens pilosa.

7. Use according to claim 6, characterized in that, The concentration of stepholidine is 0.00019531 g / L to 0.05 g / L.

8. Use according to claim 4, characterized in that, The dosage form of the herbicide can be a powder, a water dispersible granule, a water dispersible tablet, a wettable powder, an emulsifiable concentrate, an emulsion, a suspoemulsion, a microcapsule suspending agent, a granular emulsion or a soluble liquid.

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