A method for preparing humanized infant formula milk powder and functional milk powder

Abstract

The present application provides a method and an apparatus for preparing humanized infant formula milk powder and functional milk powder. The method includes: separating liquid raw milk, calculating and determining a mixing ratio based on the component substance content in the separated fluid to make it humanized or functionalized, and remixing and drying into powder at least one of de-β-lactoglobulin and zero-sugar milk protein fluid, β-lactoglobulin fluid, milk fat globule membrane protein fluid, antibacterial protein fluid, ribonuclease-osteopontin fluid, lactose fluid, sialic acid-lactose fluid, galactooligosaccharide-sialic acid fluid, mineral salt fluid, α-lactalbumin fluid, casein fluid, α-casein fluid, β-casein fluid, and liquid raw milk. This method can maximize the retention of the biological functional activities of various components, each fluid can be directly used for preparing milk powder without excessive processing, a formula calculation model is used to determine the mixing ratio of each fluid component, and milk powder can be made by one-time spray drying.

Description

Technical Field

[0001] This application relates to the technical field of dairy product processing, specifically to a method and device for preparing humanized infant formula milk powder and functional milk powder. More specifically, it relates to humanized infant formula milk powder and functional milk powder, and a device and method for preparing humanized infant formula milk powder and functional milk powder. Background Art

[0002] Liquid raw material animal milk contains a variety of bioactive substances with very low contents but rich nutritional value and also present in breast milk, such as lactoferrin, lactoperoxidase protein, β-casein, κ-casein, α-lactalbumin, milk fat globule membrane protein, osteopontin, lysozyme protein, lactoperoxidase protein, mucin, phospholipids, ribonuclease, peptone, inorganic minerals, vitamins, etc., which have extremely high biological nutritional utilization value. However, in the existing methods for obtaining various products or nutritional components based on liquid raw material milk, some operating means will cause some bioactive substances in the liquid raw material milk (such as inorganic minerals, vitamins, etc.) to be removed or inactivated. Therefore, the nutritional value of the obtained products still needs to be improved.

[0003] There are also significant differences in the components between liquid raw material milk and breast milk, and it also contains various components that are not present in breast milk, which may cause allergic reactions. In GB10766-2021 National Food Safety Standard for Larger Infant Formula Foods and GB10765-2022 National Food Safety Standard for Infant Formula Foods, it is required that whey protein accounts for respectively not less than 40% and more than 60% of the protein, lactose accounts for not less than 90% of the total carbohydrates (excluding oligosaccharides), and ash content is less than 4%. Therefore, milk powder cannot be used directly because the proportion of its whey protein is far less than 40%, and the ash content is greater than 5%, and there is no casein in whey powder for infant formula.

[0004] Currently, infant formula is produced by adding various lactose or lactose-containing demineralized whey powder, milk powder, and oligosaccharides to fresh milk in proportion, mixing, dissolving, homogenizing, and then spray-drying into powder. In the prior art, the methods for preparing infant milk powder or functional milk powder mainly include the dry powder addition method. For example, low-ash lactose powder, demineralized whey powder, milk powder, whey protein powder containing sugar macropeptide, high-purity lactoferrin powder, α-lactalbumin powder, and milk fat protein powder obtained by overprocessing are directly added to fresh raw milk to adjust the ratios of casein, whey protein, and lactose in carbohydrates and increase the contents of certain breast milk components, thus preparing infant milk powder. The above dry powders have all been overprocessed, and their biological functional activities have been damaged or reduced due to multiple high-temperature spray-drying processes during the preparation, resulting in lower nutritional value of the prepared infant milk powder or functional milk powder. Moreover, since each component can only be added but not excluded, the added dry powder raw materials are not pure single components but composite components containing non-breast milk components (such as sugar macropeptide, α-casein, and β-lactoglobulin, etc.). In addition, in order to purify the above dry powder raw materials from fresh raw milk and add them as dry powder, a large amount of processing and complex operation steps are often required. Taking lactoferrin as an example, the content of lactoferrin in fresh raw milk is about 0.07 g of lactoferrin in 1 kg of fresh raw milk, and there is only 7 kg of lactoferrin in every 100 tons of fresh raw milk. Therefore, when extracting lactoferrin with a purity of 95%, a large amount of resin is required for adsorption treatment and a large amount of salt solution is required for desorption treatment. A large-scale membrane process is also needed for rinsing and concentration, and then drying into powder. The salty wastewater generated during the purification process will cause environmental pollution, and a large amount of other bioactive components originally contained in the raw milk are also lost during the above treatment process and high-temperature spray-drying. Similarly, even when extracting lactose with a content as high as 4% in milk, in order to meet the requirement of extracting 0.3 - 0.6% of ultra-low ash, a large amount of membrane separation, electrodialysis, and ion exchange resin are required, and finally concentration and crystallization are carried out to remove a large amount of minerals and water from a solution with an ash content of 6 - 8% to obtain 0.3% low-ash lactose or demineralized whey powder. At the same time, a large amount of lactose waste liquid with extremely high COD (chemical oxygen demand) is also generated, causing environmental pollution. A large amount of energy consumption is also required to dry it into powder. Therefore, how to retain the components identical to breast milk to the greatest extent, reduce or remove the components not present in breast milk, or prepare breast milk-based infant formula and functional milk powder with high nutritional value still needs further exploration.

[0005] Therefore, the current methods and devices for preparing breast milk-based infant formula and functional milk powder still need to be improved. Summary of the Invention

[0006] In view of the deficiencies of the prior art, the object of the present invention is to provide a method and device for preparing breast milk-based infant formula and functional formula from protein and sugar fluid rather than spray-dried dry powder, so as to alleviate or even solve at least one of the problems raised in the above background art to at least a certain extent.

[0007] In one aspect of the present invention, the present invention provides a method for preparing breast milk-based infant formula and functional formula, the method comprising:

[0008] Performing a first separation treatment on liquid raw milk to obtain cream and a first protein fluid;

[0009] Performing a second separation treatment on the first protein fluid to obtain a milk fat globule membrane protein fluid and a second protein fluid;

[0010] Performing a third separation treatment on the second protein fluid to obtain a native antibacterial protein fluid and a third protein fluid;

[0011] Performing a fourth separation treatment on the third protein fluid to obtain a ribonuclease-osteopontin fluid and a fourth protein fluid;

[0012] Performing a first acid treatment on the fourth protein fluid to separate a supernatant containing β-lactoglobulin and a milk protein precipitate;

[0013] Performing a reconstitution treatment on the milk protein precipitate to obtain a β-lactoglobulin-depleted and zero-sugar milk protein fluid;

[0014] Performing a membrane filtration treatment on the supernatant to separate at least one of a β-lactoglobulin fluid, a sialic acid-lactose fluid, a lactose fluid, a galactooligosaccharide-sialic acid fluid, and a mineral salt fluid;

[0015] Performing a second acid treatment on at least part of the β-lactoglobulin-depleted and zero-sugar milk protein fluid to separate an α-lactalbumin fluid and a casein precipitate;

[0016] Performing a second reconstitution treatment on the casein precipitate to obtain a casein fluid;

[0017] Performing a separation treatment on at least part of the casein fluid to obtain an α-casein fluid and a β-casein fluid;

[0018] Based on the content of component substances in the separated fluid, calculate and determine the mixing ratio to make it breast-milk-like or functionalize it, and remix and dry into powder at least one of the de-β-lactoglobulin and zero-sugar milk protein fluid, β-lactoglobulin fluid, milk fat globule membrane protein fluid, antibacterial protein fluid, ribonuclease-osteopontin fluid, lactose fluid, sialic acid-lactose fluid, galactooligosaccharide-sialic acid fluid, mineral salt fluid, α-lactalbumin fluid, casein fluid, α-casein fluid, β-casein fluid and the liquid raw milk.

[0019] Furthermore, the liquid raw milk includes milk, dairy products or a combination thereof; the milk includes raw milk; the dairy products include whey, whole milk powder, skim milk powder, whey powder, whey protein powder or a combination thereof; the raw milk includes cow milk, goat milk, camel milk, horse milk, donkey milk, yak milk or a combination thereof.

[0020] Furthermore, the remixing and drying into powder includes: calculating according to the breast milk composition and ratio by the breast milk formula calculation model, remixing and drying into breast milk-like infant formula milk powder, or calculating according to the functional milk powder composition and ratio by the functional formula calculation model, remixing and drying into functional milk powder; wherein, the drying includes vacuum freeze-drying, spray drying or a combination thereof.

[0021] Furthermore, the method further includes adding functional components to the remixing-obtained mixed fluid and then drying into powder.

[0022] Even further, the functional components include vegetable oil, DHA, ARA or a combination thereof.

[0023] Furthermore, the first separation treatment includes defatting treatment.

[0024] Furthermore, the second separation treatment includes treating with a filter membrane having a molecular weight cut-off of 500KD - 0.2μm.

[0025] Furthermore, the third separation treatment includes gradient adsorption and elution treatment of the antibacterial protein-containing fluid with a cation resin to obtain the native antibacterial protein fluid.

[0026] Even further, the antibacterial protein-containing fluid includes at least one of the liquid raw milk, the first protein fluid, the second protein fluid, the de-β-lactoglobulin and zero-sugar milk protein fluid.

[0027] Even further, the native antibacterial protein fluid contains at least one of antibacterial protein lysozyme, oxidase, lactoferrin, IgG.

[0028] Furthermore, the fourth separation treatment includes adsorption and elution treatment with an anion resin.

[0029] Furthermore, the membrane filtration treatment includes: performing a first filtration treatment on the supernatant to separate a retentate of milk fat globule membrane protein components and a filtrate containing β-lactoglobulin, and the filter membrane for the first filtration treatment has a cut-off molecular weight of 500KD - 0.2μm.

[0030] Furthermore, the membrane filtration treatment includes: performing a second filtration treatment on the filtrate containing β-lactoglobulin to separate β-lactoglobulin fluid and a filtrate containing lactose, and the filter membrane for the second filtration treatment has a cut-off molecular weight of 500D - 10KD.

[0031] Furthermore, the membrane filtration treatment includes: performing a third filtration treatment on the filtrate containing lactose to separate sialic acid-lactose fluid and a filtrate containing lactose and mineral salts, and the filter membrane for the third filtration treatment has a cut-off molecular weight of 400D - 600D.

[0032] Furthermore, the membrane filtration treatment includes: performing a fourth filtration treatment on the filtrate containing lactose and mineral salts to separate lactose fluid and a filtrate containing mineral salts, and the filter membrane for the fourth filtration treatment has a cut-off molecular weight of 150D - 350D.

[0033] Furthermore, the membrane filtration treatment includes: performing reverse osmosis treatment on the filtrate containing mineral salts to separate mineral salt fluid and purified water.

[0034] Furthermore, the method further includes the step of converting part of the lactose-sialic acid fluid into galactooligosaccharide-sialic acid fluid.

[0035] Furthermore, calculating and determining the mixing ratio based on the component substance contents in the separated fluids includes:

[0036] determining the content of the first component substance and the content of the second component substance in each fluid, where the first component substance includes at least one of total solids, total nitrogen protein, fat, ash, and total sugar, and the second component substance includes at least one of casein, whey protein, lactose, galactooligosaccharide, bovine native oligosaccharide, sialic acid, phospholipid, and glyceride;

[0037] determining the first mixing ratio of each fluid based on the first preset parameters of the breast milk-based infant formula and functional milk powder and the content of the first component substance in each fluid;

[0038] modifying the first mixing ratio based on the second preset parameters of the breast milk-based infant formula and functional milk powder and the content of the second component substance in each fluid to determine the mixing ratio.

[0039] Further, calculating and determining the mixing ratio based on the component substance content in the separated fluid further includes:

[0040] Determine the content of the third component substance in each fluid, where the third component substance includes at least one of lysozyme, lactoferrin, lactoperoxidase, osteopontin, transcobalamin-bound cobalamin, ribonuclease, β-lactoglobulin, α-lactalbumin, milk immunoglobulin, serum protein, peptone, glycomacropeptide, milk fat globule membrane protein, α-casein, β-casein, κ-casein, other caseins, iron, calcium, sodium, phosphorus, and potassium;

[0041] Based on the third preset parameter of the breast milk-based infant formula and the functional milk powder, and the content of the third component substance in each fluid, further correct the first mixing ratio to determine the mixing ratio.

[0042] In another aspect of the present invention, the present invention provides a breast milk-based infant formula, and the breast milk-based infant formula is prepared by the above method.

[0043] In yet another aspect of the present invention, the present invention provides a functional milk powder, and the functional milk powder is prepared by the above method and includes at least one of infant milk powder, pregnant women's milk powder, middle-aged and elderly milk powder, and athlete milk powder.

[0044] In still another aspect of the present invention, the present invention provides a device for preparing breast milk-based infant formula and functional milk powder, and the device includes a separation unit, a control unit, a mixing unit, a drying unit, and a fluid storage unit; the separation unit is connected to the fluid storage unit; the fluid storage unit, the mixing unit, and the drying unit are connected in sequence, and the control unit is connected to the fluid storage unit.

[0045] Further, the separation unit includes a liquid milk inlet and a separation product outlet, and the separation product outlet is connected to the fluid storage unit.

[0046] Further, the fluid storage unit includes a plurality of fluid storage tanks, and the fluid storage unit is electrically connected to the control unit; wherein, the fluid storage tank includes a separation product inlet and a fluid outlet.

[0047] Further, the mixing unit includes a fluid inlet and a mixing product outlet; the fluid inlet is connected to the fluid storage tank, and the mixing product outlet is connected to the drying unit.

[0048] Further, the drying unit includes a mixing product inlet and a milk powder outlet; and the drying unit includes a spray drying element, a vacuum freeze drying element, or a combination thereof.

[0049] Further, the separation unit further includes a first separation component, a second separation component, a third separation component, a fourth separation component, a first acid treatment component, a membrane filtration treatment component, a redissolution component, a second acid treatment component, and a precipitate product treatment component.

[0050] Furthermore, the first separation component, the second separation component, the third separation component, and the fourth separation component are connected in sequence.

[0051] Furthermore, the first acid treatment component is connected to the fourth separation component, the membrane filtration treatment component, and the redissolution component respectively.

[0052] Furthermore, the redissolution component is connected to the second acid treatment component.

[0053] Furthermore, the second acid treatment component is connected to the precipitate product treatment component.

[0054] Generally speaking, the present invention has at least one of the following beneficial effects:

[0055] 1. The method for preparing breast milk-like infant formula and functional milk powder of the present invention performs exclusion separation and extraction on the components of liquid raw milk, can obtain various fluid components with relatively high economic value, improve the utilization rate of liquid raw milk, and fully retain the biological activities of various components during the extraction process;

[0056] 2. The method for preparing breast milk-like infant formula and functional milk powder of the present invention does not require excessive processing of the separated fluid components, can be directly used for preparing milk powder, improves production efficiency and enhances the nutritional value of milk powder;

[0057] 3. The method for preparing breast milk-like infant formula and functional milk powder of the present invention determines the mixing ratio of each fluid component by using a formula calculation model based on the nutrient content in the separated fluid components, and can produce milk powder through one-time spray drying, maximally retaining the biological functional activities of various components, reducing the loss of biological functional activities of trace proteins and other nutritional components caused by excessive processing spray drying, improving efficiency, and reducing costs;

[0058] 4. The device for preparing breast milk-like infant formula and functional milk powder of the present invention improves the utilization rate of liquid raw milk, directly uses the separated fluid components to prepare milk powder, reduces production costs, is convenient for continuous operation, and is suitable for industrial production;

[0059] 5. The device and method for preparing humanized infant formula milk powder and functional milk powder according to the present invention fully separate liquid raw milk, calculate and determine the mixing ratio based on the component content in the separated fluid, make it humanized or functionalized, and produce milk powder through one-time spray drying, retaining the biological functional activities of various components, reducing the number of processing steps, lowering production costs, saving energy and protecting the environment, and generating no three wastes.

[0060] The above summary is only for the purpose of the specification and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments and features described above, further aspects, embodiments and features of the present application will be readily apparent by reference to the drawings and the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0061] In the drawings, unless otherwise specified, the same reference numerals throughout the several views denote the same or similar components or elements. These drawings are not necessarily drawn to scale. It should be understood that these drawings only depict some embodiments disclosed in the present application and should not be regarded as limiting the scope of the present application.

[0062] Figure 1 is a flowchart of a method according to an embodiment of the present invention;

[0063] Figure 2 is a schematic structural diagram of a device according to an embodiment of the present invention;

[0064] Figure 3 is a partial structural diagram of a device according to an embodiment of the present invention;

[0065] Figure 4 is a partial structural diagram of a device according to an embodiment of the present invention.

[0066] Description of reference numerals in the drawings: 1000 - separation unit, 1100 - inlet for liquid raw milk, 1200 - outlet for separated product, 2000 - control unit, 3000 - fluid storage unit, 4000 - mixing unit, 4100 - fluid inlet, 4200 - outlet for mixed product, 5000 - drying unit, 5100 - inlet for mixed product, 5200 - outlet for milk powder, 100 - first separation component, 110 - outlet for cream, 120 - outlet for first protein fluid, 200 - second separation component, 210 - inlet for first protein fluid, 220 - outlet for milk fat globule membrane protein fluid, 230 - outlet for second protein fluid, 300 - third separation component, 310 - inlet for second protein fluid, 320 - outlet for native antibacterial protein fluid, 330 - outlet for third protein fluid, 400 - fourth separation component, 410 - inlet for third protein fluid, 420 - outlet for ribonuclease-osteopontin fluid, 430 - outlet for fourth protein fluid, 500 - first acid treatment component, 510 - inlet for liquid milk, 520 - inlet for first acidic solution, 530 - outlet for milk protein precipitate, 540 - outlet for supernatant containing β-lactoglobulin, 600 - membrane filtration treatment component, 610 - inlet for supernatant containing β-lactoglobulin, 620 - outlet for filtered product, 621 - outlet for macromolecular component retentate, 622 - outlet for β-lactoglobulin fluid, 623 - outlet for sialic acid-lactose fluid, 624 - outlet for lactose fluid, 625 - outlet for mineral salt fluid, 626 - outlet for purified water, 630 - first filtration module, 640 - second filtration module, 650 - third filtration module, 660 - fourth filtration module, 670 - reverse osmosis module, 700 - reconstitution component, 710 - inlet for milk protein precipitate, 720 - inlet for solvent, 730 - outlet for milk protein fluid without β-lactoglobulin and zero sugar, 800 - second acid treatment component, 810 - inlet for milk protein fluid without β-lactoglobulin and zero sugar, 820 - inlet for second acidic solution, 830 - outlet for α-lactalbumin fluid, 840 - outlet for casein precipitate, 900 - precipitate product treatment component, 910 - inlet for casein precipitate, 920 - outlet for casein product fluid. Detailed implementation manners

[0067] In order to more clearly understand the technical features, objectives, and beneficial effects of the present invention, the technical solution of the present invention will now be further described in detail. In the following text, only certain exemplary embodiments are briefly described. As those skilled in the art can recognize, the described embodiments can be modified in various different ways without departing from the spirit or scope of the present application. Therefore, the drawings and the description are considered to be exemplary in nature and not restrictive.

[0068] In one aspect of the present invention, the present invention provides a method for preparing breast milk-like infant formula and functional milk powder. Refer to Figure 1, the method includes separating liquid raw milk to obtain various fluids; calculating and determining the mixing ratio based on the component substance content in the separated fluids to make it breast milk-like or functionalized; and remixing and drying at least one of the separated fluids into powder according to the determined mixing ratio. Specifically, the method includes performing a first separation treatment on the liquid raw milk to obtain cream and a first protein fluid; performing a second separation treatment on the first protein fluid to obtain a milk fat globule membrane protein fluid and a second protein fluid; performing a third separation treatment on the second protein fluid to obtain a native antibacterial protein fluid and a third protein fluid; performing a fourth separation treatment on the third protein fluid to obtain a ribonuclease-osteopontin fluid and a fourth protein fluid; performing a first acid treatment on the fourth protein fluid to separate a supernatant containing β-lactoglobulin and a milk protein precipitate; performing a reconstitution treatment on the milk protein precipitate to obtain a β-lactoglobulin-depleted and zero-sugar milk protein fluid; performing a membrane filtration treatment on the supernatant containing β-lactoglobulin to separate at least one of a β-lactoglobulin fluid, a sialic acid-lactose fluid, a lactose fluid, a galactooligosaccharide-sialic acid fluid, and a mineral salt fluid; performing a second acid treatment on at least part of the β-lactoglobulin-depleted and zero-sugar milk protein fluid to separate an α-lactalbumin fluid and a casein precipitate; performing a second reconstitution treatment on the casein precipitate to obtain a casein fluid; performing a separation treatment on at least part of the casein fluid to obtain an α-casein fluid and a β-casein fluid; calculating and determining the mixing ratio based on the component substance content in the separated fluids to make it breast milk-like or functionalized, and remixing and drying at least one of the β-lactoglobulin-depleted and zero-sugar milk protein fluid, the β-lactoglobulin fluid, the milk fat globule membrane protein fluid, the antibacterial protein fluid, the ribonuclease-osteopontin fluid, the lactose fluid, the sialic acid-lactose fluid, the galactooligosaccharide-sialic acid fluid, the mineral salt fluid, the α-lactalbumin fluid, the casein fluid, the α-casein fluid, the β-casein fluid, and the liquid raw milk into powder. The method has at least one of the following beneficial effects: it can retain the biological functional activities of various components to the greatest extent, each fluid can be directly used for preparing milk powder without excessive processing, a formula calculation model is used to determine the mixing ratio of the components of each fluid, and milk powder can be made by one-time spray drying.

[0069] For the convenience of understanding, the principle by which the method can achieve the above beneficial effects is briefly described below: The present invention processes liquid raw milk, extracts its applied components through an exclusion separation method, and obtains multiple fluid components with high economic value through multiple treatments. The materials obtained through multiple separation operations can achieve the separation of the above components in a fluid state. Therefore, it is possible to avoid the destruction of active components in liquid raw milk caused by operations such as high-temperature treatment and drying, and retain the biological activities of various components. The entire process flow produces no waste and no by-products (such as glycomacropeptide, etc.). The separated fluid components can be directly used to prepare milk powder, avoiding the loss of the biological functional activities of trace proteins and other nutritional components caused by overprocessing. The operation is convenient and the production cost is reduced. In addition, the method of the present invention first measures the content of nutrients in the separated fluid components, and then uses a formula calculation model to determine the mixing ratio of each fluid component. The milk powder can be made through one spray drying, retaining the biological functional activities of various components to the greatest extent and reducing the loss of the biological functional activities of trace proteins and other nutritional components caused by overprocessing. Thus, the present invention can provide a method for preparing infant formula milk powder and functional milk powder through one drying treatment, making the prepared milk powder have higher nutritional value and more comprehensive active components, changing the current situation in the prior art of preparing infant formula milk powder and functional milk powder by the dry powder addition method through high-temperature spray drying and at least two high-temperature spray dryings. In addition, the method proposed by the present invention is flexible in operation, strong in adjustability, and can determine the mixing ratio of each fluid component according to the content of each component in the desired milk powder. The method is simple in operation and high in production efficiency.

[0070] In addition, it should be noted that the type of liquid raw milk referred to in this application is not particularly limited. Specifically, the liquid raw milk includes, but is not limited to, milk, dairy products, or a combination thereof; among them, dairy products include, but are not limited to, whey, whole milk powder, skim milk powder, whey powder, and whey protein powder. And when using the above-mentioned powdery raw materials, pure water can be added thereto for reconstitution into a liquid for treatment, or milk and / or liquid dairy products can be added thereto for reconstitution before subsequent treatment. However, it should be noted that when using a mixture of powdery raw materials or liquid raw materials, the concentration of each nutrient component in the formed liquid raw milk can be adjusted by controlling the amount of pure water or milk added to be suitable for subsequent separation treatment. In addition, milk includes raw milk, among which the type of raw milk is not particularly limited and can be cow milk, goat milk, camel milk, horse milk, donkey milk, yak milk, etc. that have been treated or untreated after milk collection. For example, raw milk can include cow milk, goat milk, camel milk, horse milk, donkey milk, yak milk, etc. that have only been sterilized after milk collection, cow milk, goat milk, camel milk, horse milk, donkey milk, yak milk, etc. that have not been treated at all after milk collection, cow milk, goat milk, camel milk, horse milk, donkey milk, yak milk, etc. that have been sterilized and clarified after milk collection, cow milk, goat milk, camel milk, horse milk, donkey milk, yak milk, etc. that have been sterilized and / or clarified and refrigerated after milk collection, and cow milk, goat milk, camel milk, horse milk, donkey milk, yak milk, etc. that have been directly refrigerated after milk collection. Among them, the methods of milk collection, sterilization treatment, and clarification treatment are all conventional methods in the art, and the refrigeration treatment conditions and time are not particularly limited as long as the raw milk does not deteriorate. In the specific implementation manner, the raw milk is preferably cow milk, goat milk, camel milk, horse milk, donkey milk, yak milk, or a combination thereof that has been sterilized and clarified after milk collection. Among them, the sterilization method and temperature can be conventional methods and temperatures in the art.

[0071] Those skilled in the art can understand that when the liquid raw milk is formed by skim milk, skim milk powder, whey powder, whey protein powder, etc., the method may not include the step of first separating the liquid raw milk to obtain cream: there is not a large amount of fat in skim milk, skim milk powder, whey powder, whey protein powder, etc., so the second separation treatment can be directly performed on them to obtain milk fat globule membrane protein fluid and the second protein fluid. In the step of remixing and drying into powder, additional fat can be supplemented or not according to needs.

[0072] For the sake of simple description, the following takes raw milk as the liquid raw milk as an example to describe the method in detail:

[0073] According to an embodiment of the present invention, the method includes performing a first separation process on liquid raw milk. The first separation process includes performing a defatting process on the liquid raw milk to separate out cream, resulting in a first protein fluid without cream. Among them, the defatting process includes using a cream centrifuge or a cream separator to separate the liquid raw milk, obtaining cream and a first protein fluid. Among them, the first protein fluid includes skim milk. In a specific implementation manner, the cream can be injected into a cream storage tank for storage and used to make foods such as cakes, ice creams, yogurts, and beverages, or can be simply post-treated and packaged and sold as a salable product.

[0074] According to an embodiment of the present invention, the method includes performing a second separation process on the first protein fluid. The second separation process includes separating out the macromolecular components (i.e., milk fat globule membrane proteins) in the first protein fluid, resulting in a milk fat globule membrane protein fluid and a second protein fluid. In a specific implementation manner, the second separation process includes using a filter membrane to process the first protein fluid. Among them, the filter membrane used can have a cut-off molecular weight of 500KD - 0.2μm, and the retentate obtained by filtration is the milk fat globule membrane protein fluid, and the filtrate obtained is the second protein fluid.

[0075] According to an embodiment of the present invention, the method includes performing a third separation process on the second protein fluid. The third separation process includes performing an ion exchange process on it to separate out the native antibacterial proteins contained therein, so as to obtain a native antibacterial protein fluid and a third protein fluid. Specifically, the third separation process includes using a cation resin to perform gradient adsorption and elution treatment on the second protein fluid. In addition, in a specific implementation manner, the third separation process may further include performing gradient adsorption and elution treatment on the antibacterial protein-containing fluid to obtain a native antibacterial protein fluid. Among them, the type of the antibacterial protein-containing fluid is not particularly limited as long as it contains antibacterial proteins. For example, in a specific implementation manner, the antibacterial protein-containing fluid includes but is not limited to at least one of liquid raw milk, the first protein fluid, the second protein fluid, de-β-lactoglobulin, and a zero-sugar milk protein fluid. In addition, the native antibacterial protein fluid includes antibacterial proteins such as lysozyme, oxidase, lactoferrin, IgG, or a combination thereof.

[0076] According to an embodiment of the present invention, the method includes performing a fourth separation process on a third protein fluid, and the fourth separation process includes performing an ion exchange process on the third protein fluid to separate ribonuclease-osteopontin contained therein, so as to obtain a ribonuclease-osteopontin fluid and a fourth protein fluid. Specifically, the fourth separation process includes adsorbing and eluting the third protein fluid using an anion resin. The treatment can be first carried out in an anion exchange resin with a pH of 5-9.5, and then eluted and collected at an acidic pH of 2-3.5, and the eluate is concentrated and enriched with a ribonuclease-osteopontin fluid using a filter membrane with a molecular weight cut-off of 20 kD. The obtained product can be used to prepare dairy products, or directly vacuum freeze-dried or spray-dried into a powdered product. Among them, the time for collecting the eluate and the process parameters can be adopted by conventional methods in the art. The time for the ion exchange resin treatment is not particularly limited. Specifically, the treatment time can be determined according to the solution content, for example, 1-5 h. The type of anion exchange resin used is not particularly limited. For example, it can include polystyrene-type resin, polyacrylic acid-type resin, phenolic resin or a combination thereof.

[0077] According to an embodiment of the present invention, the number of times of the first separation process, the second separation process, the third separation process, and the fourth separation process is not particularly limited, and each can be carried out once or multiple times until sufficient separation is achieved. In addition, in the specific implementation manner, the treatment order of the first separation process, the second separation process, the third separation process, and the fourth separation process is not particularly limited. For example, the separation of milk fat globule membrane protein, the separation of cream, the separation of ribonuclease-osteopontin, or the separation of native antibacterial protein can be prioritized. That is to say, the separation order of milk fat globule membrane protein, cream, ribonuclease-osteopontin, and native antibacterial protein is not particularly limited, and the separation order can be determined according to specific requirements.

[0078] According to an embodiment of the present invention, the method includes performing a first acid treatment on the fourth protein fluid to separate its components, obtaining a supernatant containing β-lactoglobulin and a milk protein precipitate containing the remaining protein components. In some examples, the first acid treatment includes adding an acidic solution to the fourth protein fluid until a suspension is rapidly formed in the fourth protein fluid, and then performing a filtration process to separate the supernatant containing β-lactoglobulin and the milk protein precipitate, where the time for rapidly forming the suspension does not exceed 5 s.

[0079] In addition, in the specific embodiments, the filtration treatment includes using a filtration component for filtration, and the type of the filtration component is not particularly limited, including but not limited to filter membranes, filter meshes, and filters. According to an embodiment of the present invention, the first acid treatment includes treating a fourth protein fluid to obtain a supernatant containing β-lactoglobulin and a milk protein precipitate, wherein the supernatant containing β-lactoglobulin includes β-lactoglobulin, peptone, and a small amount of other whey proteins; the milk protein precipitate includes casein and whey proteins, and the whey proteins include α-lactalbumin, serum albumin, immunoglobulins, lactoferrin, etc. In addition, the supernatant containing β-lactoglobulin and the milk protein precipitate may further include at least one of lactose and mineral salts. In addition, the first acid treatment further includes treating the liquid raw milk and the protein fluid obtained by performing at least one of the first separation treatment, the second separation treatment, the third separation treatment, and the fourth separation treatment on the liquid raw milk.

[0080] According to an embodiment of the present invention, the type of the acidic solution used in the first acid treatment is not particularly limited as long as it is food-grade. For example, the acidic solution includes inorganic acids, organic acids, or a combination thereof. In the specific embodiments, the acidic solution may include sulfuric acid, hydrochloric acid, phosphoric acid, citric acid, acetic acid, lactic acid, or a combination thereof.

[0081] According to an embodiment of the present invention, the content of the acidic solution added in the first acid treatment is not particularly limited as long as the fourth protein fluid can be rapidly formed into a suspension within 5 s and the turbidity of the suspension is not lower than 5 NTU. Specifically, the content of the acidic solution added can be determined according to the volume of the fourth protein fluid. For example, a small amount of the fourth protein fluid can be used for testing to calculate the required content of the acidic solution. And the temperature range for performing the first acid treatment is 4 - 35 °C.

[0082] According to an embodiment of the present invention, the number of times of the first acid treatment is not particularly limited and can be performed once or multiple times. For example, in the specific embodiments, the obtained milk protein precipitate can be dissolved and the first acid treatment can be repeated, and the steps of separating the filtrate and the precipitate can be repeated multiple times to fully separate β-lactoglobulin.

[0083] According to an embodiment of the present invention, after the fourth protein fluid is rapidly formed into a suspension within 5 s, it is filtered to obtain a supernatant containing β-lactoglobulin and a milk protein precipitate. The inventors found that usually, the separation of proteins is carried out according to the isoelectric point of the proteins. An acidic solution is added to the protein solution to reach a specific pH value, so as to achieve the separation of various proteins. However, due to the similar isoelectric points of some proteins, effective separation cannot be carried out. For example, the isoelectric points of β-lactoglobulin and α-lactalbumin in whey protein are similar, and it is difficult to separate them using the existing technology. That is to say, the acid separation method that determines the pH according to the isoelectric point cannot fully separate the components of the fourth protein fluid. β-Lactoglobulin is a non-breast milk component and has a certain risk of sensitization, and is not suitable for preparing infant formula milk powder. However, β-lactoglobulin can be used to assist in muscle growth and is a protein nutrient required by fitness enthusiasts. Therefore, if β-lactoglobulin and α-lactalbumin can be separated in a certain treatment step, on the one hand, the application value of the obtained milk-based product can be improved, and on the other hand, the amount of separated β-lactoglobulin can be increased, thereby further improving the economic benefit of the method and the extraction rate of the nutritional substances in the liquid raw milk. The inventors found that when an acidic solution is added until a suspension is rapidly formed and then filtered, the proportion of β-lactoglobulin in the obtained supernatant is high, and the separation of β-lactoglobulin and other protein components can be achieved. The remaining protein components are concentrated in the precipitate and can be extracted through subsequent separation treatments. Thus, the difficult-to-separate β-lactoglobulin can be extracted in advance to obtain a supernatant containing β-lactoglobulin and a milk protein precipitate.

[0084] According to an embodiment of the present invention, the method includes redissolving the milk protein precipitate obtained by the first acid treatment to obtain a β-lactoglobulin-free and zero-sugar milk protein fluid, and the β-lactoglobulin-free and zero-sugar milk protein fluid can be used to make β-lactoglobulin-free dairy products. Among them, redissolving includes adding a solvent to the milk protein precipitate to dissolve it, and the type of the added solvent is not particularly limited as long as it is food-grade, and it can be an acidic solution, a basic solution or a neutral solution; the content of the added solvent is not particularly limited as long as the precipitate can be dissolved; in addition, the number of times of adding the solvent is not particularly limited, and it can be added at one time or divided into multiple times. In a specific embodiment, the pH of the β-lactoglobulin-free and zero-sugar milk protein fluid obtained by redissolving is not particularly limited, for example, it can be 5-10.

[0085] According to an embodiment of the present invention, the method includes subjecting the separated supernatant containing β-lactoglobulin to membrane filtration treatment to separate one or more of β-lactoglobulin fluid, sialic acid-lactose fluid, lactose fluid, galactooligosaccharide-sialic acid fluid, and mineral salt fluid. The membrane filtration treatment includes a first filtration treatment, a second filtration treatment, a third filtration treatment, a fourth filtration treatment, and reverse osmosis treatment or a combination thereof, and the time and number of times of the first filtration treatment, the second filtration treatment, the third filtration treatment, the fourth filtration treatment, and the reverse osmosis treatment are not particularly limited and can be carried out once or multiple times. Specifically, the treatment time and number of times can be determined according to the volume of the solution to be treated and the size of the filter membrane. In addition, the molecular weight cut-off of the filter membrane used in the first filtration treatment is greater than that of the filter membrane used in the second filtration treatment, the molecular weight cut-off of the filter membrane used in the second filtration treatment is greater than that of the filter membrane used in the third filtration treatment, and the molecular weight cut-off of the filter membrane used in the third filtration treatment is greater than that of the filter membrane used in the fourth filtration treatment.

[0086] It should be specifically noted here that during the membrane filtration process, the selection, operation sequence, treatment time, and other parameters of the first filtration treatment, second filtration treatment, third filtration treatment, fourth filtration treatment, and reverse osmosis treatment can be adjusted according to the specific composition of the protein product to be obtained. Those skilled in the art can use familiar separation lines to separate the supernatant containing β-lactoglobulin as needed. For example, in a specific embodiment, the supernatant containing β-lactoglobulin can be subjected to the first filtration treatment to obtain a macromolecular component retentate and a filtrate containing β-lactoglobulin. The macromolecular component retentate includes the remaining unseparated milk fat globule membrane proteins, and the filtrate containing β-lactoglobulin includes β-lactoglobulin, lactose, sialic acid, and mineral salts; the obtained filtrate containing β-lactoglobulin is subjected to the second filtration treatment to obtain a filtrate containing lactose and a retentate, and the obtained retentate is the β-lactoglobulin fluid; the obtained filtrate containing lactose is subjected to the third filtration treatment to obtain a sialic acid-lactose fluid and a filtrate containing lactose and mineral salts; the obtained filtrate containing lactose and mineral salts is subjected to the fourth filtration treatment to obtain a lactose fluid and a filtrate containing mineral salts; then the filtrate containing mineral salts is subjected to reverse osmosis treatment to obtain a mineral salt fluid and purified water. In addition, the first filtration treatment includes treatment with a filter membrane having a molecular weight cut-off of 500KD - 0.2μm, the second filtration treatment includes treatment with a filter membrane having a molecular weight cut-off of 500D - 10KD, the third filtration treatment includes treatment with a filter membrane having a molecular weight cut-off of 400D - 600D, the fourth filtration treatment includes treatment with a filter membrane having a molecular weight cut-off of 150D - 350D, and the reverse osmosis treatment includes treatment with a reverse osmosis membrane. In addition, the obtained mineral salt component and purified water can both be added back to the dairy product for recycling. The obtained β-lactoglobulin fluid can be directly used to prepare dairy products or dried into powder in addition to being dried into powder after re-determining the mixing ratio in the subsequent process.

[0087] According to an embodiment of the present invention, the method may optionally include the step of converting a part of the lactose-sialic acid fluid into a galactooligosaccharide-sialic acid fluid. Specifically, lactose hydrolase can be added to the separated lactose-sialic acid fluid to hydrolyze lactose into galactooligosaccharides to obtain a galactooligosaccharide-sialic acid fluid. It should be noted that in a specific embodiment, the third filtration treatment, fourth filtration treatment, and reverse osmosis treatment can be used for any separated fluid component to separate lactose fluid, mineral salts, purified water, etc.

[0088] According to an embodiment of the present invention, the present invention further includes subjecting at least part of the reconstituted β-lactoglobulin and zero-sugar milk protein fluid to a second acid treatment to separate it into an α-lactalbumin fluid and a casein precipitate. Among them, the α-lactalbumin fluid can be used to prepare dairy products or directly transported to be dried into α-lactalbumin powder. In addition, the type of acidic solution used in the second acid treatment, the content of the added acidic solution, and the treatment temperature are the same as those in the first acid treatment, and will not be elaborated here.

[0089] In addition, the present invention further includes obtaining a casein fluid from the casein precipitate obtained by the second acid treatment. For example, the obtained casein precipitate can be directly subjected to a second reconstitution treatment to obtain a casein fluid. The type of solvent used in the second reconstitution treatment is not particularly limited and can be an acidic solution, a basic solution, or a neutral solution; the content of the added solvent is also not particularly limited as long as the precipitate can be dissolved; in addition, the number of times of adding the solvent is not particularly limited and can be added at one time or divided into multiple times. In a specific embodiment, the pH of the reconstituted casein fluid is not particularly limited and can be, for example, 7-10.

[0090] According to an embodiment of the present invention, the present invention further includes subjecting at least part of the obtained casein-containing fluid to ion exchange resin treatment or adding a metal salt solution thereto to separate α-casein and β-casein to obtain an α-casein fluid and a β-casein fluid, wherein the metal salt includes but is not limited to sodium chloride, sodium sulfate, sodium bicarbonate, calcium chloride, calcium hydrogen phosphate, calcium bicarbonate, calcium sulfate, ferric chloride, ferrous chloride, ferric sulfate, ferrous sulfate, or a combination thereof. Preferably, the metal salt includes calcium chloride. The temperature is 0-8 degrees. Since α-casein is also not present in breast milk, separating it out is beneficial for preparing infant formula or other infant dairy products.

[0091] Thus, the liquid raw milk undergoes a full liquid phase treatment, and molecules such as native polypeptides, peptones, sialic acid, vitamins, inorganic minerals, and bovine milk oligosaccharide BMO in the liquid raw milk are retained throughout the separation process. Bioactive functional components such as milk fat globule membrane proteins, milk lectins, mucin, immunoglobulins, lactoferrin, osteopontin, and lysozyme are also retained, without generating waste. The treatment conditions are mild and do not damage bioactive substances. The separated components are in a liquid state, and various milk-based raw material fluids are obtained, including de-β-lactoglobulin and zero-sugar milk protein fluids, β-lactoglobulin fluids, milk fat globule membrane protein fluids, antibacterial protein fluids, ribonuclease-osteopontin fluids, lactose fluids, sialic acid-lactose fluids, galactooligosaccharide-sialic acid fluids, mineral salt fluids, α-lactalbumin fluids, casein fluids, α-casein fluids, and β-casein fluids. The various active nutrients in these fluids are not damaged and can be directly mixed and proportioned according to the nutritional requirements of the product to obtain various milk powders, reducing the processing steps and increasing the nutritional value of the obtained milk powders. In addition, it should be noted that the separated fluids are not highly purified monomeric fluids and may contain other substances such as proteins, fats, ash, and sugars, thus avoiding excessive separation processing and optimizing the separation processing efficiency and cost.

[0092] According to the embodiments of the present invention, the present invention further includes calculating and determining the mixing ratio based on the component substance contents in the separated fluids described above, making its components close to breast milk or functionalizing it, and remixing and drying at least one of the separated de-β-lactoglobulin and zero-sugar milk protein fluids, β-lactoglobulin fluids, milk fat globule membrane protein fluids, antibacterial protein fluids, ribonuclease-osteopontin fluids, lactose fluids, sialic acid-lactose fluids, galactooligosaccharide-sialic acid fluids, mineral salt fluids, α-lactalbumin fluids, casein fluids, α-casein fluids, and β-casein fluids into powder according to the calculated and determined mixing ratio.

[0093] Specifically, the content of the component substances in each separated fluid can be detected in advance, and then the formula calculation model can be calculated according to the composition and proportion of the target product. Among them, the detection methods for the content of the first component substance, the second-stage component substance, and the third component substance are not particularly limited as long as they can be detected. For example, in the specific implementation manner, the content of the first component substance can be detected by using the corresponding national standard detection method, the content of the second component substance and the third component substance can be detected by RP-HPLC to obtain a chromatogram and calculated by the normalization method, and the content of sialic acid can be detected by fluorescence method. For example, in the specific implementation manner, the breast milk formula calculation model can be calculated according to the composition of breast milk and the proportional relationship of each component to determine the types of the finally mixed fluids and the content of each fluid, and the determined fluids can be remixed and dried into breast milk-based infant formula. Or the functional formula calculation model can be calculated according to the composition of the functional milk powder and the proportional relationship of each component to determine the types of the finally mixed fluids and the content of each fluid, and the determined fluids can be remixed and dried into functional milk powder. In addition, the method for drying the mixed material obtained after mixing is not particularly limited as long as the function of drying into powder can be achieved. For example, drying includes but is not limited to vacuum freeze-drying, spray-drying, or a combination thereof.

[0094] According to an embodiment of the present invention, during the process of remixing and drying, functional components can be further added to the mixed fluid obtained by remixing and dried into powder together. Specifically, the types and contents of the added functional components can be determined according to the content of the required nutrients. Among them, the types of the functional components are not particularly limited as long as they have certain nutritional value. For example, in the specific implementation manner, they include but are not limited to functional proteins, compound vitamins, compound mineral elements, compound vegetable oils, or a combination thereof, such as including vegetable oils, DHA, ARA, or a combination thereof.

[0095] According to an embodiment of the present invention, the present invention further includes calculating and determining a mixing ratio based on the content of component substances in the separated fluid. Specifically, calculating the content of component substances in each separated fluid includes determining the content of the first component substance and the content of the second component substance in each fluid, so as to determine the mixing ratio of each fluid based on the measured content of the component substances. Among them, the first component substance includes total solids, total nitrogen protein, fat, ash, total sugar or a combination thereof; the second component substance includes casein, whey protein, lactose, galactooligosaccharide, bovine native oligosaccharide, sialic acid, phospholipid, glyceride or a combination thereof. Calculating and determining the mixing ratio includes determining a first preset parameter and a second preset parameter based on the target components of the infant formula or functional formula. Among them, the first preset parameter corresponds to the content of the first component substance in the target components of the infant formula or functional formula, and the second preset parameter corresponds to the content of the second component substance in the target components of the infant formula or functional formula. Specifically, after measuring the content of the component substances in the separated fluid, the type and content of the mixed fluid can be initially determined based on the first preset parameter in the target infant formula or functional formula to determine the first mixing ratio; on the basis of meeting the first preset parameter, the first mixing ratio that has been determined can be further corrected based on the second preset parameter in the target infant formula or functional formula to determine the final mixing ratio.

[0096] In addition, in the specific implementation manner, calculating the content of the component substances in each separated fluid may further include determining the content of the third component substance in each fluid, so as to better determine the mixing ratio of each fluid based on the measured content of the component substances. Specifically, the third component substance includes lysozyme, lactoferrin, lactoperoxidase, osteopontin, transcobalamin-binding cobalamin, ribonuclease, β-lactoglobulin, α-lactalbumin, milk immunoglobulin, serum protein, peptone, glycomacropeptide, milk fat globule membrane protein, α-casein, β-casein, κ-casein, other caseins, iron, calcium, sodium, phosphorus, potassium or a combination thereof. And calculating and determining the mixing ratio may further include further determining a third preset parameter based on the target components of the infant formula or functional formula, where the third preset parameter corresponds to the content of the third component substance in the target components of the infant formula or functional formula. That is to say, in the specific implementation manner, after the first mixing ratio that has been determined has been corrected based on the second preset parameter in the target infant formula or functional formula, the first mixing ratio that has been determined can be further corrected based on the third preset parameter in the target infant formula or functional formula to determine the final mixing ratio. Among them, adjusting the mixing ratio based on the second preset parameter and the third preset parameter enables more precise adjustment of the composition of the target infant formula or functional formula.

[0097] That is to say, in the specific implementation, after separating the liquid raw milk to obtain various component fluids, the content of the first component substance, the second component substance, the third component substance or a combination thereof in each fluid can be measured in advance; then, based on the composition of the target infant formula or functional milk powder to be obtained, the first preset parameter, the second preset parameter, the third preset parameter or a combination thereof is determined. Finally, based on the determined first preset parameter, second preset parameter, third preset parameter or a combination thereof, and the content of the first component substance, the second component substance, the third component substance or a combination thereof in each fluid measured, the mixing ratio of each fluid is determined. And the mixing ratio can be adjusted based on the second preset parameter and the third preset parameter to obtain an infant formula or functional milk powder with the desired components.

[0098] For example, in the specific implementation, the types and contents of the fluids to be mixed can be initially determined by first determining the contents and proportional relationships of total solids, total nitrogen protein, fat, ash, and total sugar in the target infant formula or functional milk powder, and the first mixing ratio is determined. Then, based on the first mixing ratio, the determined first mixing ratio is corrected or adjusted according to the content and proportional relationship of at least one of casein, whey protein, lactose, galactooligosaccharide, bovine native oligosaccharide, sialic acid, phospholipid, and glyceride in the target infant formula or functional milk powder to be obtained, so as to obtain the final mixing ratio. Or further correct or adjust the determined first mixing ratio according to the content and proportional relationship of at least one of lysozyme, lactoferrin, lactoperoxidase, osteopontin, transcobalamin-bound cobalamin, ribonuclease, β-lactoglobulin, α-lactalbumin, milk immunoglobulin, serum protein, peptone, glycomacropeptide, milk fat globule membrane protein, α-casein, β-casein, κ-casein, other caseins, iron, calcium, sodium, phosphorus, and potassium in the target infant formula or functional milk powder to be obtained to determine the final mixing ratio.

[0099] According to an embodiment of the present invention, the method can be used for, but not limited to, preparing breast-milk-like infant formula and functional milk powder, and can also be used for preparing other dairy products. For example, the separated fluid components are used to prepare milk powder or liquid milk that meet various component requirements. In addition, the fluid components separated by the present invention can also be directly dried into powder, or dried into powder after rinsing.

[0100] In another aspect of the present invention, a breast-milk-like infant formula is provided, and the breast-milk-like infant formula is prepared by using the above method. The obtained breast-milk-like infant formula has components close to breast milk, and bioactive substances such as inorganic minerals, vitamins, sialic acid, and other protein components are fully retained. The processing steps are few and the milk powder is made only through one drying step.

[0101] In yet another aspect of the present invention, the present invention provides a functional milk powder prepared by the above method, and the obtained functional milk powder has high nutritional value. Moreover, the type of the functional milk powder is not particularly limited as long as it can have certain functions, such as, but not limited to, infant milk powder, pregnant women's milk powder, middle-aged and elderly milk powder, athlete milk powder, and other functional milk powders.

[0102] In still another aspect of the present invention, the present invention provides a device for preparing breast milk-like infant formula milk powder and functional milk powder. Referring to Figure 2 , the device includes a separation unit 1000, a control unit 2000, a mixing unit 4000, a drying unit 5000, and a fluid storage unit 3000. Among them, the separation unit 1000 is connected to the fluid storage unit 3000 to transport the separated fluid to the fluid storage unit 3000 for storage; and the fluid storage unit 3000, the mixing unit 4000, and the drying unit 5000 are connected in sequence, and the control unit 2000 is connected to the fluid storage unit 3000 to control the quantitative transportation of each fluid in the fluid storage unit 3000 to the mixing unit 4000 according to the calculated re-mixing ratio. The device has at least one of the following beneficial effects: improving the efficiency of preparing milk powder, reducing the production cost, facilitating continuous operation, and being suitable for industrial production.

[0103] The device can prepare breast milk-like infant formula milk powder and functional milk powder according to the method described above.

[0104] The principle by which the device can achieve the above beneficial effects is briefly described below: The separation unit 1000 of the device can fully separate the liquid raw milk to obtain a variety of fluids with high nutritional value, and the obtained various fluids flow into the fluid storage unit 3000 for storage; the control unit 2000 can measure the content of component substances in each fluid in the fluid storage unit 3000, and can set preset parameters determined according to the composition of the desired target infant formula milk powder or functional milk powder; then, according to the preset parameters and the measured content of component substances in each fluid, the mixing ratio of each fluid is calculated, and each fluid inside the fluid storage unit 3000 is transported to the mixing unit 4000 for mixing according to the measured mixing ratio to obtain a mixed material, and finally the obtained mixed material is transported to the drying unit 5000 for drying treatment to make milk powder with the target components.

[0105] According to an embodiment of the present invention, in the device, the separation unit 1000 includes a liquid raw milk inlet 1100 and a separation product outlet 1200. Among them, the separation product outlet 1200 includes a plurality of fluid outlets, for example, a milk protein fluid outlet for removing β-lactoglobulin and zero sugar, a β-lactoglobulin fluid outlet, a milk fat globule membrane protein fluid outlet, an antibacterial protein fluid outlet, a ribonuclease-osteopontin fluid outlet, a lactose fluid outlet, a sialic acid-lactose fluid outlet, a galactooligosaccharide-sialic acid fluid outlet, a mineral salt fluid outlet, an α-lactalbumin fluid outlet, a casein fluid outlet, an α-casein fluid outlet, and a β-casein fluid outlet. And each separation product outlet 1200 is connected to the fluid storage unit 3000. The fluid storage unit 3000 is connected to the control unit 2000 and includes a plurality of fluid storage tanks. Each fluid storage tank independently includes a separation product inlet and a fluid outlet. Among them, each separation product outlet 1200 in the separation unit 1000 is connected to the corresponding fluid storage tank.

[0106] According to an embodiment of the present invention, the mixing unit 4000 includes a fluid inlet 4100 and a mixing product outlet 4200. Among them, the mixing unit 4000 is connected to each fluid storage tank in the fluid storage unit 3000, and the number of fluid inlets 4100 is not particularly limited and can be one or more. That is to say, the fluid outlets on the fluid storage tanks in the fluid storage unit 3000 are all connected to the fluid inlets 4100 on the mixing unit 4000 to transport the fluid into the mixing unit 4000 for mixing. In addition, the mixing product outlet 4200 of the mixing unit 4000 is connected to the drying unit 5000 to dry the mixing product to make milk powder. In a specific embodiment, the drying unit 5000 includes a mixing product inlet 5100 and a milk powder outlet 5200, so that the mixing product generated by the mixing unit 4000 flows from the mixing product outlet 4200 into the drying unit 5000 for drying treatment. There are drying elements in the drying unit 5000, and the types of the drying elements are not particularly limited as long as they can play a drying role. For example, the drying elements can include but are not limited to spray drying elements, vacuum freeze-drying elements, or combinations thereof, and preferably include freeze-drying elements.

[0107] According to an embodiment of the present invention, the specific structure of the separation unit 1000 is briefly described below. Refer to Figure 3, the separation unit 1000 includes a first separation component 100, a second separation component 200, a third separation component 300, a fourth separation component 400, a first acid treatment component 500, a membrane filtration treatment component 600, a reconstitution component 700, a second acid treatment component 800, and a precipitate product treatment component 900. Among them, the first separation component 100, the second separation component 200, the third separation component 300, and the fourth separation component 400 are connected in sequence, and the first acid treatment component 500 is respectively connected to the fourth separation component 400, the membrane filtration treatment component 600, and the reconstitution component 700, and the second acid treatment component 800 is respectively connected to the reconstitution component 700 and the precipitate product treatment component 900.

[0108] According to an embodiment of the present invention, the first separation component 100 includes a liquid raw milk inlet 1100, a first protein fluid outlet 120, and a cream outlet 110. The cream outlet 110 can be connected to a cream storage tank, and the first separation component 100 includes a defatting assembly, and the defatting assembly includes, but is not limited to, a cream centrifuge or a cream separator. The second separation component 200 includes a first protein fluid inlet 210, a second protein fluid outlet 230, and a milk fat globule membrane protein fluid outlet 220. Among them, the first protein fluid inlet 210 is connected to the first separation component 100, and the second separation component 200 includes a filter membrane assembly with a cut-off molecular weight of 500KD - 0.2μm. The third separation component 300 includes a second protein fluid inlet 310, a third protein fluid outlet 330, and a native antibacterial protein fluid outlet 320. Among them, the second protein fluid inlet 310 is connected to the second separation component 200, and the third separation component 300 includes a cation exchange resin. The fourth separation component 400 includes a third protein fluid inlet 410, a fourth protein fluid outlet 430, and a ribonuclease-osteopontin fluid outlet 420. Among them, the third protein fluid inlet 410 is connected to the third separation component 300, and the fourth separation component 400 includes an anion exchange resin.

[0109] In addition, the fourth separation component 400 is connected to the first acid treatment component 500 to transport the fourth protein fluid generated in the fourth separation component 400 to the first acid treatment component 500 for treatment. And in the specific implementation manner, the connection order of the first separation component 100, the second separation component 200, the third separation component 300, and the fourth separation component 400 is not particularly limited. Specifically, the connection order of the above components can be adjusted according to the order of the fluid components to be separated. In the embodiment of the present application, the components are connected in the order of the first separation component 100, the second separation component 200, the third separation component 300, and the fourth separation component 400, but this connection order is not limited in the specific implementation manner. However, it should be noted that the separation component set at the end should be connected to the first acid treatment component 500, and in addition to treating the fourth protein fluid, the first acid treatment component 500 can also treat other liquid milk.

[0110] According to an embodiment of the present invention, the first acid treatment component 500 includes a liquid milk inlet 510, a first acidic solution inlet 520, a milk protein precipitate outlet 530, and a supernatant outlet 540 containing β-lactoglobulin. Among them, the milk protein precipitate outlet 530 is connected to the reconstitution component 700, and the supernatant outlet 540 containing β-lactoglobulin is connected to the membrane filtration treatment component 600. In the specific implementation manner, the fourth protein fluid or other liquid milk flows in from the liquid milk inlet 510, and the acidic solution flows in from the first acidic solution inlet 520, and the two rapidly form a suspension in the first acid treatment component 500. In addition, a filtration component is provided in the first acid treatment component 500 to perform filtration treatment on the rapidly formed suspension to separate a supernatant containing β-lactoglobulin and a milk protein precipitate. And the obtained supernatant containing β-lactoglobulin flows out from the supernatant outlet 540 containing β-lactoglobulin, and the obtained milk protein precipitate flows out from the milk protein precipitate outlet 530. Among them, the type of the filtration component for filtration is not particularly limited as long as it can separate the precipitate and the supernatant. Specifically, the filtration component includes but is not limited to a filter membrane, a filter mesh, and a filter.

[0111] According to an embodiment of the present invention, the reconstitution component 700 is used to reconstitute the generated milk protein precipitate, and it includes a milk protein precipitate inlet 710, a solvent inlet 720, and a milk protein fluid outlet 730 for de-β-lactoglobulin and zero-sugar. In the specific implementation manner, the milk protein precipitate from the first acid treatment component 500 flows into the reconstitution component 700 from the milk protein precipitate inlet 710, and the solvent flows in from the solvent inlet 720 to reconstitute the milk protein precipitate to form a milk protein fluid for de-β-lactoglobulin and zero-sugar.

[0112] According to an embodiment of the present invention, the separation unit 1000 may further include a second acid treatment component 800, which is connected to the redissolution component 700 to perform a second acid treatment on the de-β-lactoglobulin and zero-sugar milk protein fluid generated in the redissolution unit. Among them, the second acid treatment component 800 includes an inlet 810 for the de-β-lactoglobulin and zero-sugar milk protein fluid, a second acidic solution inlet 820, an α-lactalbumin fluid outlet 830, and a casein precipitate outlet 840. The de-β-lactoglobulin and zero-sugar milk protein fluid generated by redissolution is injected into the second acid treatment component 800, and the acidic solution flows in from the second acidic solution inlet 820 and reacts rapidly with the de-β-lactoglobulin and zero-sugar milk protein fluid to form a suspension. In addition, the second acid treatment component 800 also has a filtration component to filter the formed suspension, and the type of the filtration component is not particularly limited, including but not limited to filter membranes, filter meshes, and filters.

[0113] According to an embodiment of the present invention, the separation unit 1000 may further include a precipitate product treatment component 900. Among them, the precipitate product treatment component 900 is connected to the casein precipitate outlet 840 and has a casein precipitate inlet 910 and a casein product fluid outlet 920 to treat the casein precipitate to obtain a casein product fluid, where the casein product fluid includes at least one of casein fluid, α-casein fluid, and β-casein fluid.

[0114] According to an embodiment of the present invention, the membrane filtration treatment component 600 is connected to the supernatant outlet 540 containing β-lactoglobulin for filtering and separating the supernatant containing β-lactoglobulin. The membrane filtration treatment component 600 includes a supernatant inlet 610 containing β-lactoglobulin and one or more filtration product outlets 620, and has at least one of a first filtration component 630, a second filtration component 640, a third filtration component 650, a fourth filtration component 660, and a reverse osmosis component 670. In addition, the numbers of the first filtration component 630, the second filtration component 640, the third filtration component 650, the fourth filtration component 660, and the reverse osmosis component 670 are not particularly limited and may all include one or more.

[0115] In the specific implementation, refer to Figure 4, the membrane filtration treatment component 600 may include a first filtration component 630, a second filtration component 640, a third filtration component 650, a fourth filtration component 660, and a reverse osmosis component 670. Among them, the first filtration component 630, the second filtration component 640, the third filtration component 650, the fourth filtration component 660, and the reverse osmosis component 670 are connected in sequence, and have a macromolecular component retentate outlet 621, a β-lactoglobulin fluid outlet 622, a sialic acid-lactose fluid outlet 623, a lactose fluid outlet 624, a mineral salt fluid outlet 625, and a purified water outlet 626. Specifically, the supernatant containing β-lactoglobulin flows into the membrane filtration treatment component 600 shown in Figure 4 . The macromolecular component retentate separated at the first filtration component 630 flows out from the macromolecular retentate outlet; the remaining β-lactoglobulin filtrate is injected into the second filtration component 640, and the separated β-lactoglobulin fluid flows out from the β-lactoglobulin fluid outlet 622; the remaining lactose-containing filtrate is injected into the third filtration component 650, and the separated sialic acid-lactose fluid flows out from the sialic acid-lactose fluid outlet 623; the remaining lactose- and mineral salt-containing filtrate is injected into the fourth filtration component 660, and the separated lactose fluid flows out from the lactose fluid outlet 624; finally, the mineral salt-containing filtrate such as is injected into the reverse osmosis component 670, the separated mineral salt fluid flows out from the mineral salt fluid outlet 625, and the purified water flows out from the purified water outlet 626. Among them, the first filtration component 630 includes a filter membrane with a molecular weight cut-off of 500KD - 0.2μm, the second filtration component 640 includes a filter membrane with a molecular weight cut-off of 500D - 10KD, the third filtration component 650 includes a filter membrane with a molecular weight cut-off of 400D - 600D, the fourth filtration component 660 includes a filter membrane with a molecular weight cut-off of 150D - 350D, and the reverse osmosis component 670 includes a reverse osmosis membrane.

[0116] Example

[0117] The method proposed by the present invention will be described in detail below through specific examples. The methods used in the following examples are all conventional methods unless otherwise specified, and the reagents used are all commercially available reagents unless otherwise specified.

[0118] Example 1

[0119] In this embodiment, a breast milk-based infant formula is prepared using the method and device of the present invention. The breast milk characteristics of this formula are as follows: whey protein accounts for 70%, the dry weight ratio of total sugar is 70%, and lactose accounts for 94% of the total sugar. Moreover, it is enriched with α-lactalbumin, antibacterial proteins (lysozyme, lactoferrin, oxidase protein, immunoglobulin), β-casein, milk fat globule membrane protein, osteopontin, ribonuclease, peptone, sialic acid, and native oligosaccharides, while having low levels of β-lactoglobulin, low α-casein, low ash content, and no glycomacropeptide. The specific formula components of the breast milk-based infant formula expected to be obtained in this embodiment are shown in Table 1 below.

[0120] In this embodiment, skim milk with the component substances shown in Table 2 below is used as the base, and the separated fluid components are added to 2100 kg of this skim milk and mixed for spray drying to make a breast milk-based infant formula. In this embodiment, the method of the present invention is used to separate raw milk, and the component substance contents in the separated fluid components are analyzed. The results are shown in Tables 3 - 13 below. According to the analysis results, the specific amounts of each fluid to be added are determined as follows: 3000 kg of lactose fluid, 310 kg of galacto-oligosaccharide-sialic acid fluid, 100 kg of milk fat globule membrane protein fluid, 20 kg of antibacterial protein fluid, 320 kg of α-lactalbumin fluid, 20 kg of ribonuclease-osteopontin fluid, 80 kg of β-lactoglobulin-free and zero-sugar milk protein fluid, and 220 kg of β-casein fluid. A breast milk-based infant formula with the component contents shown in Table 1 is obtained.

[0121] Note: In Tables 1 - 13 below: Total solids include fat (phospholipids and glycerides), total nitrogen protein includes casein and whey protein, and total sugar includes lactose and oligosaccharides. db% represents the dry weight percentage; TP% represents the percentage in total nitrogen protein.

[0122] Table 1 Formula Components of the Target Infant Formula

[0123]

[0124]

[0125]

[0126] Table 2 Component Substance Contents in Skim Milk

[0127]

[0128]

[0129]

[0130] In this embodiment, the raw milk is separated by the method of the present invention to obtain lactose fluid, galactooligosaccharide-sialic acid fluid, milk fat globule membrane protein fluid, antibacterial protein fluid, α-lactalbumin fluid, ribonuclease-osteopontin fluid, de-β-lactoglobulin and zero-sugar milk protein fluid, β-casein fluid, β-lactoglobulin fluid, casein fluid and α-casein fluid. The contents of the component substances in the above fluid components are analyzed, and the results are shown in Tables 3-13 below:

[0131] Table 3 Contents of Component Substances in Lactose Fluid

[0132]

[0133] Table 4 Contents of Component Substances in Galactooligosaccharide-Sialic Acid Fluid

[0134]

[0135]

[0136] Table 5 Contents of Component Substances in Milk Fat Globule Membrane Protein Fluid

[0137]

[0138]

[0139]

[0140] Table 6 Contents of Component Substances in Antibacterial Protein Fluid

[0141]

[0142]

[0143]

[0144] Table 7 Contents of Component Substances in Ribonuclease-Osteopontin Fluid

[0145]

[0146]

[0147] Table 8 Contents of Component Substances in α-Lactalbumin Fluid

[0148]

[0149]

[0150] Table 9 Contents of Component Substances in De-β-Lactoglobulin and Zero-Sugar Milk Protein Fluid

[0151]

[0152]

[0153]

[0154] Component content of substances in 10β-casein fluid

[0155]

[0156]

[0157]

[0158] Component content of substances in 11β-lactoglobulin fluid

[0159]

[0160]

[0161]

[0162] Component content of substances in 12 casein fluid

[0163]

[0164]

[0165]

[0166] Component content of substances in 13α-casein fluid

[0167]

[0168]

[0169]

[0170] Generally speaking, the device and method for preparing humanized infant formula milk powder and functional milk powder proposed by the present invention fully separate liquid raw milk, calculate and determine the mixing ratio based on the component content of substances in the separated fluid, make it humanized or functionalized, and make milk powder through one-time spray drying, retaining the biological functional activity of various components, reducing the number of processing times, reducing production costs, saving energy and protecting the environment, producing no three wastes, improving the utilization rate of liquid raw milk, facilitating continuous operation, and being suitable for industrial production.

[0171] The foregoing has shown and described the basic principles, main features and advantages of the present invention. For a person skilled in the art, it is obvious that the present invention is not limited to the details of the above-mentioned exemplary embodiments, and without departing from the spirit or basic features of the present invention, the present invention can be implemented in other specific forms. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting.

[0172] In addition, it should be understood that although this specification is described in terms of embodiments, not every embodiment only contains an independent technical solution. This narrative manner of the specification is only for clarity. Those skilled in the art should regard the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A method for preparing humanized infant formula and functional milk powder, characterized in that, The method includes: Performing a first separation treatment on liquid raw milk to obtain cream and a first protein fluid; Performing a second separation treatment on the first protein fluid to obtain a milk fat globule membrane protein fluid and a second protein fluid; Performing a third separation treatment on the second protein fluid to obtain a native antibacterial protein fluid and a third protein fluid; Performing a fourth separation treatment on the third protein fluid to obtain a ribonuclease-osteopontin fluid and a fourth protein fluid; Performing a first acid treatment on the fourth protein fluid to separate a supernatant containing β-lactoglobulin and a milk protein precipitate. The first acid treatment is to add an acidic solution to rapidly form a suspension within 5 seconds in the fourth protein fluid, and the turbidity of the suspension is not less than 5 NTU; Performing a reconstitution treatment on the milk protein precipitate to obtain a β-lactoglobulin-depleted and zero-sugar milk protein fluid; Performing a membrane filtration treatment on the supernatant to separate at least one of a β-lactoglobulin fluid, a sialic acid-lactose fluid, a lactose fluid, a galactooligosaccharide-sialic acid fluid, and a mineral salt fluid; Performing a second acid treatment on at least part of the β-lactoglobulin-depleted and zero-sugar milk protein fluid to separate an α-lactalbumin fluid and a casein precipitate; Performing a second reconstitution treatment on the casein precipitate to obtain a casein fluid; Performing a separation treatment on at least part of the casein fluid to obtain an α-casein fluid and a β-casein fluid; Based on the component substance content in the separated fluids, calculating and determining the mixing ratio to make it breast milk-like or functional, and remixing and drying at least one of the β-lactoglobulin-depleted and zero-sugar milk protein fluid, β-lactoglobulin fluid, milk fat globule membrane protein fluid, antibacterial protein fluid, ribonuclease-osteopontin fluid, lactose fluid, sialic acid-lactose fluid, galactooligosaccharide-sialic acid fluid, mineral salt fluid, α-lactalbumin fluid, casein fluid, α-casein fluid, β-casein fluid, and the liquid raw milk into powder.

2. The method according to claim 1, characterized in that, The liquid raw milk includes milk, dairy products, or a combination thereof; The milk includes raw milk; The dairy products include whey, whole milk powder, skim milk powder, whey powder, whey protein powder, or a combination thereof; The raw milk includes cow milk, goat milk, camel milk, horse milk, donkey milk, yak milk, or a combination thereof.

3. The method according to claim 1, wherein The remixing and drying into powder includes: Performing a breast milk-like formula calculation model calculation according to the breast milk composition and ratio, and remixing and drying into a breast milk-like infant formula powder, or Performing a functional formula calculation model calculation according to the functional milk powder composition and ratio, and remixing and drying into a functional milk powder; Wherein, the drying includes vacuum freeze-drying, spray-drying, or a combination thereof.

4. The method according to claim 1, characterized in that The method further includes adding a functional component to the remixing-obtained mixed fluid and then drying into powder; The functional component includes vegetable oil, DHA, ARA, or a combination thereof.

5. The method according to claim 1, characterized in that, The method satisfies at least one of the following conditions: The first separation treatment includes defatting treatment; The second separation treatment includes using a filter membrane with a molecular weight cut-off of 500 KD - 0.2 μm for treatment; The third separation treatment includes gradient adsorption and elution treatment of the antibacterial protein-containing fluid with a cation resin to obtain a native antibacterial protein fluid; The fourth separation treatment includes adsorption and elution treatment with an anion resin; The antibacterial protein-containing fluid includes at least one of the liquid raw milk, the first protein fluid, the second protein fluid, the de-β-lactoglobulin, and the zero-sugar milk protein fluid; The native antibacterial protein fluid contains at least one of antibacterial protein lysozyme, oxidase, lactoferrin, and IgG.

6. The method according to claim 1, characterized in that, The membrane filtration treatment includes at least one of the following treatments: Performing a first filtration treatment on the supernatant to separate a macromolecular component retentate and a β-lactoglobulin-containing filtrate, and the filter membrane for the first filtration treatment has a cut-off molecular weight of 500KD-0.2μm; Performing a second filtration treatment on the β-lactoglobulin-containing filtrate to separate a β-lactoglobulin fluid and a lactose-containing filtrate, and the filter membrane for the second filtration treatment has a cut-off molecular weight of 500D-10KD; Performing a third filtration treatment on the lactose-containing filtrate to separate a sialic acid-lactose fluid and a lactose and mineral salt-containing filtrate, and the filter membrane for the third filtration treatment has a cut-off molecular weight of 400D-600D; Performing a fourth filtration treatment on the lactose and mineral salt-containing filtrate to separate a lactose fluid and a mineral salt-containing filtrate, and the filter membrane for the fourth filtration treatment has a cut-off molecular weight of 150D-350D; Performing a reverse osmosis treatment on the mineral salt-containing filtrate to separate a mineral salt fluid and purified water.

7. The method according to claim 1, characterized in that, The method further includes a step of converting a part of the lactose-sialic acid fluid into a galactooligosaccharide-sialic acid fluid.

8. The method according to claim 1, wherein Calculating and determining the mixing ratio based on the component substance contents in the separated fluids includes: Determining the content of the first component substance and the content of the second component substance in each fluid, the first component substance includes at least one of total solids, total nitrogen protein, fat, ash, and total sugar, and the second component substance includes at least one of casein, whey protein, lactose, galactooligosaccharide, bovine native oligosaccharide, sialic acid, phospholipid, and glyceride; Based on the first preset parameters of the breast-milk-based infant formula and the functional formula, and the content of the first component substance in each fluid, determining the first mixing ratio of each fluid; Based on the second preset parameters of the breast-milk-based infant formula and the functional formula, and the content of the second component substance in each fluid, correcting the first mixing ratio to determine the mixing ratio.

9. The method according to claim 8, characterized in that, Calculating and determining the mixing ratio based on the component substance contents in the separated fluids further includes: Determining the content of the third component substance in each fluid, the third component substance includes at least one of lysozyme, lactoferrin, lactoperoxidase, osteopontin, transcobalamin-binding cobalamin, ribonuclease, β-lactoglobulin, α-lactalbumin, milk immunoglobulin, serum protein, peptone, glycomacropeptide, milk fat globule membrane protein, α-casein, β-casein, κ-casein, other caseins, iron, calcium, sodium, phosphorus, and potassium; Based on the third preset parameter of the breast milk-like infant formula and functional formula, and the content of the third component substance of each fluid, the first mixing ratio is further corrected to determine the mixing ratio.

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