A multi-channel upright full-polarization microscopic imaging device

Abstract

The application relates to the field of microscopic imaging technology, and particularly discloses a multi-channel upright full-polarization microscopic imaging device, which comprises a shell provided with a mounting cavity, a non-polarization microscopic imaging assembly and a full-polarization microscopic imaging assembly, wherein the non-polarization microscopic imaging assembly and the full-polarization microscopic imaging assembly are arranged in the mounting cavity, the non-polarization microscopic imaging assembly is connected with the inner bottom wall of the shell, the full-polarization microscopic imaging assembly is connected with the non-polarization microscopic imaging assembly, and a mounting clasp is arranged on the outer bottom wall of the shell and corresponds to the position of the non-polarization microscopic imaging assembly. The application has the advantages that the clasp can be mounted on a conventional upright optical microscope to realize the non-polarization and full-polarization microscopic imaging function of the conventional upright optical microscope, and the transformation cost is effectively reduced.

Description

Technical Field

[0001] This invention relates to the field of microscopic imaging technology, and in particular to a multi-channel upright fully polarized microscopic imaging device. Background Technology

[0002] In the optical system of a traditional upright optical microscope, the microscope objectives and the microscope observation mirror are positioned above the sample stage. The sample is adjusted by a coarse and fine focusing mechanism that drives the stage to move vertically up and down, thereby adjusting the distance between the sample and the objective lens to achieve focusing for microscopic imaging. Commonly used microscope observation mirrors include monoculars, binoculars, and trinoculars. Monoculars and binoculars are mainly used for visual observation, while trinoculars have a camera output port, allowing for simultaneous visual and photographic observation or switching between spectroscopic observation.

[0003] In a conventional upright polarizing microscope, the observation mirror and the intermediate mirror group form a combination that performs the functions of polarization analysis and microscopic imaging. The polarizer in the intermediate mirror group usually adopts a graduated rotation method, that is, it obtains polarization characteristic imaging at different angles by rotating the polarizer. The module structure is relatively simple and easy to operate, but the obtained polarization characteristic information is limited and it is not suitable for polarization microscopic imaging analysis of complex or living samples.

[0004] Currently, to achieve non-polarized and fully polarized microscopic imaging on a traditional upright optical microscope, it is often necessary to modify the traditional upright optical microscope. However, this modification process is complex and costly. Summary of the Invention

[0005] To address the aforementioned problems in existing technologies, the present invention aims to provide a multi-channel upright fully polarized microscopy imaging device that can be mounted on a traditional upright optical microscope via a retaining ring, thereby enabling the traditional upright optical microscope to possess both non-polarized and fully polarized microscopy imaging functions, effectively reducing the cost of modification.

[0006] The above-mentioned technical objective of the present invention is achieved through the following technical solution:

[0007] A multi-channel upright fully polarized microscopy imaging device, comprising:

[0008] The housing has a mounting cavity;

[0009] A non-polarized microscopy imaging component and a fully polarized microscopy imaging component are provided, both of which are disposed within the mounting cavity. The non-polarized microscopy imaging component is connected to the inner bottom wall of the housing, and the fully polarized microscopy imaging component is connected to the non-polarized microscopy imaging component.

[0010] A mounting ring is disposed on the outer bottom wall of the housing and corresponds to the position of the non-polarized microscopic imaging component.

[0011] Compared to existing technologies, this application mounts the entire device onto the body of a traditional upright optical microscope by installing a retaining ring, enabling the traditional upright optical microscope to have multimodal and full polarization microscopy imaging capabilities. This allows for full utilization of the traditional upright optical microscope while effectively reducing the cost of modifying it.

[0012] As a preferred embodiment of the present invention, the non-polarization microscopic imaging component includes:

[0013] The base has a reference channel, and the mounting ring is disposed at the channel opening near the bottom wall of the housing;

[0014] A scope assembly, wherein the scope assembly is disposed within the mounting retaining ring;

[0015] A non-polarized connecting tube, one end of which is connected to the base, and a non-polarized camera is provided at the other end. The non-polarized connecting tube has a non-polarized channel, which is connected to the reference channel.

[0016] The first unpolarized beam splitter prism group is disposed within the reference channel at the location corresponding to the unpolarized channel.

[0017] As a preferred embodiment of the present invention, the fully polarized microscopic imaging component includes:

[0018] The second unpolarized beam splitter prism group is disposed in the reference channel and located above the first unpolarized beam splitter prism group.

[0019] The reflective end assembly and the transmission end assembly are both disposed on the base and are connected to the reference channel. The positions of the reflective end assembly and the transmission end assembly correspond to the positions of the second non-polarizing beam splitter prism group.

[0020] As a preferred embodiment of the present invention, the reflective end assembly includes:

[0021] A first drive motor is disposed within the reference channel and connected to the base. The rotation shaft of the first drive motor has a first polarization channel, which is connected to the reference channel.

[0022] A half-wave plate, wherein the half-wave plate is disposed within the first polarization channel;

[0023] The first image plane center adjustment frame is mounted on the base via a first connecting plate, and the position of the first image plane center adjustment frame corresponds to the position of the half-wave plate. The base is provided with an opening corresponding to the first image plane center adjustment frame so that the imaging beam enters the first image plane center adjustment frame after passing through the half-wave plate.

[0024] The first image plane distance adjustment cylinder has one end connected to the first image plane center adjustment frame via a first connecting sleeve, and the other end is equipped with a first polarization camera.

[0025] As a preferred embodiment of the present invention, a first fastening screw is provided on the first connecting sleeve. One end of the first fastening screw passes through the side wall of the first connecting sleeve and abuts against the first image plane distance adjusting cylinder. The first fastening screw is threadedly connected to the first connecting sleeve and is used to fix the position of the first image plane distance adjusting cylinder.

[0026] By adopting the above solution, the first image plane distance adjusting cylinder can be effectively fixed by the first fastening screw, thereby improving the stability of the first image plane distance adjusting cylinder during use.

[0027] As a preferred embodiment of the present invention, the transmission end assembly includes:

[0028] A second drive motor is disposed within the reference channel and connected to the base. The rotation shaft of the second drive motor has a second polarization channel, which is connected to the reference channel.

[0029] A quarter-wave plate, wherein the quarter-wave plate is disposed within the second polarization channel;

[0030] The second image plane center adjustment frame is mounted on the base via a second connecting plate, and the position of the second image plane center adjustment frame corresponds to the position of the quarter-wave plate. The base is provided with an opening corresponding to the second image plane center adjustment frame so that the imaging beam enters the second image plane center adjustment frame after passing through the quarter-wave plate.

[0031] The second image plane distance adjustment cylinder has one end connected to the second image plane center adjustment frame via a second connecting sleeve, and the other end is equipped with a second polarization camera.

[0032] As a preferred embodiment of the present invention, a second fastening screw is provided on the second connecting sleeve. One end of the second fastening screw passes through the side wall of the second connecting sleeve and abuts against the second image plane distance adjusting cylinder. The second fastening screw is threadedly connected to the second connecting sleeve and is used to fix the position of the second image plane distance adjusting cylinder.

[0033] By adopting the above solution, the second image plane distance adjusting cylinder can be effectively fixed by the second fastening screw, thereby improving the stability of the second image plane distance adjusting cylinder during use.

[0034] As a preferred embodiment of the present invention, the splitting ratio of the second non-polarizing beam splitter prism group is 50:50.

[0035] Using the above scheme, the imaging light enters the second unpolarized beam splitter group after passing through the first unpolarized beam splitter group. Setting the splitting ratio of the second unpolarized beam splitter group to 50:50 ensures that the output light intensity of the first polarization channel and the second polarization channel is consistent.

[0036] As a preferred embodiment of the present invention, the housing is provided with heat dissipation grooves and a port panel, and both the non-polarized microscopic imaging component and the fully polarized microscopic imaging component are connected to the port panel.

[0037] As a preferred embodiment of the present invention, the mounting clasp is a dovetail clasp.

[0038] By adopting the above solution, the efficiency of installation and replacement can be effectively improved by using dovetail retaining rings. At the same time, by replacing dovetail retaining rings of different sizes, the adaptability and versatility of the device can be effectively improved.

[0039] The aforementioned multi-channel upright fully polarized microscopy imaging device has the following advantages: Since traditional upright optical microscopes have significant limitations in the application of fully polarized microscopy imaging technology, this device employs a far-limit, far-imaging optical system. By selecting mounting rings compatible with existing microscope products, it can be quickly installed on the body of an upright optical microscope, forming a multi-modal, fully polarized microscopy imaging module. While fully utilizing the functional components of existing upright optical microscopes, it effectively reduces the configuration cost of fully polarized microscopes and accelerates the development and application of polarization microscopy imaging technology. Attached Figure Description

[0040] Figure 1 This is a schematic diagram of the structure of a multi-channel upright fully polarized microscopic imaging device according to the present invention;

[0041] Figure 2 This is a schematic diagram of the internal structure of a multi-channel upright fully polarized microscopic imaging device according to the present invention;

[0042] Figure 3 This is a schematic diagram of the port panel structure in a multi-channel upright fully polarized microscopic imaging device of the present invention;

[0043] In the diagram: 1. Outer shell; 2. Mounting cavity; 3. Non-polarizing microscopic imaging assembly; 31. Base; 32. Reference channel; 33. Lens assembly; 34. Non-polarizing connecting tube; 35. Non-polarizing camera; 36. Non-polarizing channel; 37. First non-polarizing beam splitter prism assembly; 38. Camera adapter interface; 4. Fully polarizing microscopic imaging assembly; 41. Second non-polarizing beam splitter prism assembly; 42. Reflecting end assembly; 421. First drive motor; 422. First polarization channel; 423. Half-wave plate; 424. First image plane center adjustment frame; 425. First image plane distance adjustment tube; 426. First connecting sleeve; 42 7. First polarizing camera; 428. First fastening screw; 429. First connecting plate; 43. Transmission end assembly; 431. Second drive motor; 432. Second polarization channel; 433. Quarter-wave plate; 434. Second image plane center adjustment frame; 435. Second image plane distance adjustment cylinder; 436. Second connecting sleeve; 437. Second polarizing camera; 438. Second fastening screw; 439. Second connecting plate; 5. Mounting retaining ring; 6. Heat dissipation groove; 7. Port panel; 8. USB / C1 interface; 9. USB / CPR interface; 10. USB / CPT interface; 11. Motor for 1 / 2 interface; 12. Motor for 1 / 4 interface.

[0044] The realization of the objective, functional features and advantages of the present invention will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0045] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0046] It should be noted that if the embodiments of the present invention involve directional indicators (such as up, down, left, right, front, back, etc.), the directional indicators are only used to explain the relative positional relationship and movement of the components in a specific posture. If the specific posture changes, the directional indicators will also change accordingly.

[0047] Furthermore, if the embodiments of this invention involve descriptions such as "first" or "second," these descriptions are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the use of "and / or" or "and / or" throughout the text includes three parallel solutions. For example, "A and / or B" includes solution A, solution B, or a solution where both A and B are satisfied simultaneously. Furthermore, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed by this invention.

[0048] This invention proposes a multi-channel upright full polarization microscopy imaging device.

[0049] Reference Figure 1 and Figure 2 In one embodiment of the present invention, a multi-channel upright fully polarized microscopy imaging device includes: a housing 1, a non-polarized microscopy imaging component 3, a fully polarized microscopy imaging component 4, and a mounting ring 5. The housing 1 has a mounting cavity 2. Both the non-polarized microscopy imaging component 3 and the fully polarized microscopy imaging component 4 are disposed within the mounting cavity 2. The non-polarized microscopy imaging component 3 is connected to the inner bottom wall of the housing 1, and the fully polarized microscopy imaging component 4 is connected to the non-polarized microscopy imaging component 3. The mounting ring 5 is disposed on the outer bottom wall of the housing 1 and is connected to the non-polarized microscopy imaging component 3. In this embodiment, the mounting ring 5 is a dovetail ring. By mounting the entire device onto the body of a traditional upright optical microscope using the mounting ring 5, the traditional upright optical microscope is equipped with multi-modal, fully polarized microscopy imaging capabilities, which fully utilizes the traditional upright optical microscope while effectively reducing the modification cost of the traditional upright optical microscope. The use of dovetail retaining rings can effectively improve the efficiency of installation and replacement. At the same time, by replacing dovetail retaining rings of different sizes, the adaptability and versatility of the device can be effectively improved.

[0050] Reference Figure 2In one embodiment, the non-polarized microscopic imaging assembly 3 includes: a base 31, a lens assembly 33, a non-polarized connecting tube 34, and a first non-polarized beam splitter prism assembly 37. The base 31 has a reference channel 32, and an opening is formed in the bottom wall of the outer casing 1. One of the channel openings of the reference channel 32 on the base 31 is located at the opening in the bottom wall of the outer casing 1. The base 31 is fixedly installed inside the outer casing 1 by bolts. A mounting ring 5 is installed on the base 31 by hexagonal socket screws, and the mounting ring 5 is positioned near the channel opening of the reference channel 32 on the bottom wall of the outer casing 1. The lens assembly 33 is installed in the mounting ring 5 via a micro-thread; one end of the non-polarizing connecting tube 34 is threaded to the base 31, and the other end is fitted with a non-polarizing camera 35 via a camera adapter interface 38. The non-polarizing connecting tube 34 has a non-polarizing channel 36, which is connected to the reference channel 32. In this embodiment, the non-polarizing camera 35 is a high-definition color camera; the first non-polarizing beam splitter prism group 37 is set in the reference channel 32 at the position corresponding to the non-polarizing channel 36. It is worth noting that the beam splitting ratio of the first non-polarizing beam splitter prism group 37 is 10:90. Since the lens assembly 33 is installed in the dovetail ring via a micro-thread, the distance between the lens assembly 33 and the first non-polarizing beam splitter prism group 37 can be adjusted by the thread to make the imaging plane of different magnification objectives consistent with the photosensitive plane of the high-definition color camera. The first polarizing camera 427 in the first polarizing channel 422 and the second polarizing camera 437 in the second polarizing channel 432 mentioned below are matched and calibrated with the image plane position of the reference channel 32 as a reference.

[0051] Reference Figure 2 In one embodiment, the fully polarized microscopic imaging component 4 includes: a second unpolarized beam splitter prism group 41, a reflective end component 42, and a transmission end component 43. The second unpolarized beam splitter prism group 41 is disposed within the reference channel 32 and located above the first unpolarized beam splitter prism group 37. The reflective end component 42 and the transmission end component 43 are both disposed on the base 31 and are both connected to the reference channel 32. The positions of the reflective end component 42 and the transmission end are corresponding to the positions of the second unpolarized beam splitter prism group 41. In this embodiment, the splitting ratio of the second unpolarized beam splitter prism group 41 is 50:50. The advantage of designing the splitting ratio to 50:50 is that when the imaging light enters the second unpolarized beam splitter prism group 41 after passing through the first unpolarized beam splitter prism group 37, setting the splitting ratio of the second unpolarized beam splitter prism group 41 to 50:50 can ensure that the output light intensity of the first polarization channel 422 and the second polarization channel 432 mentioned below is consistent.

[0052] Reference Figure 2In one embodiment, the reflector assembly 42 includes: a first drive motor 421, a half-wave plate 423, a first image plane center adjustment frame 424, and a first image plane distance adjustment cylinder 425. The first drive motor 421 is disposed in the reference channel 32 and mounted on the base 31 by bolts. The rotation shaft of the first drive motor 421 has a first polarization channel 422, which is connected to the reference channel 32. The half-wave plate 423 is fixed in the first polarization channel 422 by a pressure ring. The first image plane center adjustment frame 424 is mounted on the base 31 by a first connecting plate 429. Specifically, the first connecting plate 429 is mounted on the base 31 by screws, and the first image plane center adjustment frame 424 is mounted on the first connecting plate 429 by screws. It is worth noting that the position of the first image plane center adjustment frame 424 corresponds to the position of the half-wave plate 423. The base 31 has an opening at the position corresponding to the first image plane center adjustment frame 424 so that the imaging beam enters the first image plane center adjustment frame 424 after passing through the half-wave plate 423. One end of the first image plane distance adjustment cylinder 425 is connected to the first image plane center adjustment frame 424 through the first connecting sleeve 426, and the other end is provided with a first polarization camera 427. The first connecting sleeve 426 is installed on the first image plane center adjustment frame 424 by threads, one end of the first image plane distance adjustment cylinder 425 is inserted into the first connecting sleeve 426, and the first polarization camera 427 is connected to the first image plane distance adjustment cylinder 425 by a standard C interface thread. Furthermore, a first fastening screw 428 is threaded onto the first connecting sleeve 426. One end of the first fastening screw 428 passes through the side wall of the first connecting sleeve 426 and abuts against the first image plane distance adjusting cylinder 425. The position of the first fastening screw 428 can be adjusted by rotating it so that the first fastening screw 428 is tightened or moved away from the first image plane distance adjusting cylinder 425 to achieve the fixing and disassembly of the first image plane distance adjusting cylinder 425. In this way, the first image plane distance adjusting cylinder 425 can be fixed after its position is adjusted. The first fastening screw 428 can effectively fix the first image plane distance adjusting cylinder 425, improving the stability of the first image plane distance adjusting cylinder 425 during use. In use, the position of the first image plane distance adjusting cylinder 425 in the first connecting sleeve 426 is moved to achieve the parfocality requirement of each channel image plane. After the image plane distance is determined, the first fixing screw 428 is tightened to fix the first image plane distance adjusting cylinder 425. The image plane center is adjusted by adjusting the micro-adjustment screws on both sides of the first image plane center adjustment frame 424, so that the field of view center of the first polarization channel 422 is consistent with that of other channels.

[0053] Reference Figure 2In one embodiment, the transmission end assembly 43 includes: a second drive motor 431, a quarter-wave plate 433, a second image plane center adjustment frame 434, and a second image plane distance adjustment cylinder 435. The second drive motor 431 is disposed in the reference channel 32 and mounted on the base 31 by bolts. The rotation shaft of the second drive motor 431 has a second polarization channel 432, which is connected to the reference channel 32. The quarter-wave plate 433 is fixed in the second polarization channel 432 by a pressure ring. The second image plane center adjustment frame 434 is mounted on the base 31 by a second connecting plate 439. Specifically, the second connecting plate 439 is mounted on the base 31 by screws, and the second image plane center adjustment frame 434 is mounted on the second connecting plate 439 by screws. It is worth noting that the position of the second image plane center adjustment frame 434 corresponds to the position of the quarter-wave plate 433. The base 31 has an opening at the position corresponding to the second image plane center adjustment frame 434 so that the imaging beam enters the second image plane center adjustment frame 434 after passing through the quarter-wave plate 433. One end of the second image plane distance adjustment cylinder 435 is connected to the second image plane center adjustment frame 434 through the second connecting sleeve 436, and the other end is provided with a second polarization camera 437. The second connecting sleeve 436 is threadedly installed on the second image plane center adjustment frame 434, one end of the second image plane distance adjustment cylinder 435 is inserted into the second connecting sleeve 436, and the second polarization camera 437 is threadedly connected to the second image plane distance adjustment cylinder 435 through a standard C-interface. Furthermore, a second fastening screw 438 is threaded onto the second connecting sleeve 436. One end of the second fastening screw 438 passes through the side wall of the second connecting sleeve 436 and abuts against the second image plane distance adjusting cylinder 435. The position of the second fastening screw 438 can be adjusted by rotating it so that the second fastening screw 438 is tightened or moved away from the second image plane distance adjusting cylinder 435 to achieve the fixing and disassembly of the second image plane distance adjusting cylinder 435. In this way, the second image plane distance adjusting cylinder 435 can be fixed after its position is adjusted. The second fastening screw 438 can effectively fix the second image plane distance adjusting cylinder 435, improving the stability of the second image plane distance adjusting cylinder 435 during use. In use, the position of the second image plane distance adjusting cylinder 435 in the second connecting sleeve 436 is moved to achieve the parfocality requirement of each channel image plane. After the image plane distance is determined, the second fixing screw 438 is tightened to fix the second image plane distance adjusting cylinder 435. The image plane center is adjusted by adjusting the micro-adjustment screws on both sides of the second image plane center adjustment bracket 434, so that the field of view center of the second polarization channel 432 is consistent with that of other channels.

[0054] The first polarization camera 427 and the second polarization camera 437 mentioned above are both high frame rate area array cameras, and the first drive motor 421 and the second drive motor 431 are both high-speed DC precision rotary motors. These two high frame rate area array cameras require rigorous matching calibration. During calibration, a microscope objective lens of 40x or higher is used to image the sample target reference. First, the target image is clearly imaged at the center of the field of view of the high-definition color camera, which is the non-polarized camera 35 in this scheme. Then, the high frame rate area array camera in the second polarization channel 432, i.e., the second polarization camera 437, is calibrated. Fine adjustments are made using the second image plane distance adjustment cylinder 435 and the second image plane center adjustment frame 434 to ensure that the center of the photosensitive panel of the second polarization camera 437 coincides with the center of the microscope imaging field of view and is in a synchronously clear image plane. Finally, the first polarization camera 427 in the first polarization channel 422 is calibrated using the same calibration method as the second polarization camera 437. The microscopic images obtained from the three channels—unpolarized channel 36, the first polarized channel 422, and the second polarized channel 432—are merged. Image displacement deviations are identified through a designed calibration procedure, and repeated adjustments are made to ensure the coincidence of the field of view centers of each camera is within the allowable deviation range. The aforementioned optical components are internally enclosed, effectively preventing dust from entering the internal optical system. The image distance adjustment cylinders and image plane adjustment frames of each camera are located externally on the base for easy adjustment and maintenance.

[0055] Reference Figure 3 In one embodiment, the housing 1 is provided with a heat dissipation groove 6 and a port panel 7. Both the non-polarized microscopy imaging component 3 and the fully polarized microscopy imaging component 4 are connected to the port panel 7. The port panel 7 is respectively provided with a digital camera control interface with three channels and a drive motor interface with two drive motors. The USB / C1 interface 8 is used for the non-polarized camera 35, i.e., a high-definition color camera; the USB / CPR interface 9 is used for the first polarized camera 427; the USB / CPT interface 10 is used for the second polarized camera 437; the MOTOR FOR 1 / 2 interface 11 is used for the first drive motor 421; and the MOTOR FOR 1 / 4 interface 12 is used for the second drive motor 431. It is worth noting that the USB / C1, USB / CPR, and USB / CPT interfaces are all standard USB interfaces. A precision rotating motor drives the waveplate to rotate, thereby quantifying the phase delay, and the polarization camera detects the light intensity information of the imaging beam under various polarization states.

[0056] Because traditional upright optical microscopes have significant limitations in the application of fully polarized microscopy imaging technology, this device adopts a far-limited imaging optical system. By selecting a mounting ring 5 that is compatible with existing microscope products, it can be quickly installed on the body of an upright optical microscope to form a multi-modal, fully polarized microscopy imaging module. While making full use of the functional components of existing upright optical microscopes, it effectively reduces the configuration cost of fully polarized microscopes and accelerates the development and application of polarization microscopy imaging technology.

[0057] The above description is merely a preferred embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural transformations made using the contents of the present invention's specification and drawings under the inventive concept of the present invention, or direct / indirect applications in other related technical fields, are included within the patent protection scope of the present invention.

Claims

1. A multi-channel upright-type full-polarization microscopic imaging apparatus, characterized by comprising: include: The housing has a mounting cavity; A non-polarized microscopy imaging component and a fully polarized microscopy imaging component are provided, both of which are disposed within the mounting cavity. The non-polarized microscopy imaging component is connected to the inner bottom wall of the housing, and the fully polarized microscopy imaging component is connected to the non-polarized microscopy imaging component. A mounting ring is disposed on the outer bottom wall of the housing and corresponds to the position of the non-polarizing microscopic imaging component. The mounting ring is a dovetail ring. The non-polarization microscopy imaging component includes: The base has a reference channel, and the mounting ring is disposed at the channel opening near the bottom wall of the housing; A scope assembly, wherein the scope assembly is disposed within the mounting retaining ring; A non-polarized connecting tube, one end of which is connected to the base, and a non-polarized camera is provided at the other end. The non-polarized connecting tube has a non-polarized channel, which is connected to the reference channel. The first unpolarized beam splitter prism group is disposed in the reference channel corresponding to the unpolarized channel. The polarization microscopy imaging component includes: The second unpolarized beam splitter prism group is disposed in the reference channel and located above the first unpolarized beam splitter prism group. The reflective end assembly and the transmission end assembly are both disposed on the base and are connected to the reference channel. The positions of the reflective end assembly and the transmission end assembly correspond to the positions of the second non-polarizing beam splitter prism group.

2. The multi-channel epi-type full-polarization microscopic imaging device according to claim 1, characterized in that, The reflective end assembly includes: A first drive motor is disposed within the reference channel and connected to the base. The rotation shaft of the first drive motor has a first polarization channel, which is connected to the reference channel. A half-wave plate, wherein the half-wave plate is disposed within the first polarization channel; The first image plane center adjustment frame is mounted on the base via a first connecting plate, and the position of the first image plane center adjustment frame corresponds to the position of the half-wave plate. The base is provided with an opening corresponding to the first image plane center adjustment frame so that the imaging beam enters the first image plane center adjustment frame after passing through the half-wave plate. The first image plane distance adjustment cylinder has one end connected to the first image plane center adjustment frame via a first connecting sleeve, and the other end is equipped with a first polarization camera.

3. The multi-channel epi-mode total polarization microscopic imaging device according to claim 2, characterized in that: The first connecting sleeve is provided with a first fastening screw. One end of the first fastening screw passes through the side wall of the first connecting sleeve and abuts against the first image plane distance adjusting cylinder. The first fastening screw is threadedly connected to the first connecting sleeve and is used to fix the position of the first image plane distance adjusting cylinder.

4. The multi-channel epi-mode total polarization microscopic imaging device according to claim 1, characterized in that, The transmission end assembly includes: A second drive motor is disposed within the reference channel and connected to the base. The rotation shaft of the second drive motor has a second polarization channel, which is connected to the reference channel. a quarter wave plate, which is arranged in the second polarization channel; a second image plane center adjustment frame, which is arranged on the base through a second connecting plate, and the position of the second image plane center adjustment frame corresponds to the position of the quarter wave plate, and the base is provided with an opening corresponding to the second image plane center adjustment frame to allow the imaging light beam to enter the second image plane center adjustment frame after passing through the quarter wave plate; a second image plane distance adjustment cylinder, one end of which is connected with the second image plane center adjustment frame through a second connecting sliding sleeve, and the other end is provided with a second polarization camera.

5. The multi-channel epi-mode total polarization microscopic imaging device according to claim 4, characterized in that: A second fastening screw is arranged on the second connecting sliding sleeve, one end of the second fastening screw penetrates through the side wall of the second connecting sliding sleeve and abuts against the second image plane distance adjustment cylinder, the second fastening screw is threadedly connected with the second connecting sliding sleeve, and the second fastening screw is used for fixing the position of the second image plane distance adjustment cylinder.

6. The multi-channel epi-mode total polarization microscopic imaging device according to claim 1, characterized in that: The splitting ratio of the second non-polarization beam splitting prism group is 50:

50.

7. The multi-channel epi-mode total polarization microscopic imaging device of claim 1, wherein: The shell is provided with a heat dissipation groove and a port panel, and the non-polarization microscopic imaging assembly and the full-polarization microscopic imaging assembly are connected with the port panel.

Images