Micro-cutting seedling cultivation method of Momordica grosvenori

Abstract

The invention discloses a micro-cutting seedling raising method for Momordica grosvenori, which belongs to the technical field of cutting seedling raising. The method comprises the following steps: regulating the length of stem nodes of a mother plant by using a stem control and root inducing agent, performing dark treatment to obtain pretreated vines; taking the pretreated vines as cutting materials; dipping the base of the cutting materials in yellow mud slurry, which is an antiseptic and root-inducing agent; cutting or burying the cuttings in a matrix containing vermiculite, grass charcoal soil, and pine needle segments; culturing the cuttings in an environment with a light transmittance of 10%-15%, a temperature of 25-30°C, and an air relative humidity of 80-90%, and spraying the cuttings with an aqueous solution of the stem control and root inducing agent once every 5-7 days after new buds unfold their leaves. The present invention develops a stem-controlling and root-inducing agent and an antiseptic and root-growing agent based on the growth characteristics of the winged momordica grosvenori. The stem-controlling and root-inducing agent is used to regulate the growth of stem nodes, and the light and dark treatment is used to induce the rooting of the stem nodes. The invention uses less material and has a high utilization rate, is simple to operate, and has a high survival rate. The plant can be bred from March to October, breaking the limitation that seedlings can only be raised by pressing potatoes in autumn, and solving the problem of low survival rate of the winged momordica grosvenori cuttings.

Description

Technical Field

[0001] The invention relates to the technical field of Momordica grosvenori planting, in particular to the technical field of Momordica grosvenori cutting seedling cultivation, and more particularly to a micro-cutting seedling cultivation method of Momordica grosvenori. Background Art

[0002] Siraitia siamensis (Craib) C. Jeffrey ex Zhang et D. Fang and Siraitia grosvenorii (Swingle) C. Jeffrey ex A.M. Lu et Z. Y. Zhan are both members of the Cucurbitaceae family and are both medicinal and edible, with their fruits used as medicine. They have benefits such as clearing heat and moistening the lungs, relieving sore throat and relieving pain, and promoting bowel movements. Both contain the same active ingredient, sucralose V. With a sweetness over 300 times that of sucrose, sucralose V is a high-intensity, low-calorie natural sweetener widely used in food and beverages. Guangxi boasts abundant production of S. grosvenorii, concentrated in northern Guangxi and several surrounding counties, where it is a major cash crop. Existing S. grosvenorii cultivars have very poor resistance to root-knot nematode and viral diseases, often resulting in cracked fruit, deformed fruit, small fruit, misshapen buds, withered seeds, seedling death, and harvest failure. This is exacerbated by long-term planting in the same plot. This has seriously hindered the high-quality development of the monk fruit industry. However, monk fruit has a narrow distribution, high requirements for temperature, light, water and fertilizer, and very few wild germplasm resources. Over the years, although the national and local governments have invested a lot of money in research such as new variety breeding, no breakthrough progress has been made in germplasm.

[0003] Winged Momordica grosvenori is native to the mountainous areas of southern, southwestern, and northwestern Guangxi. It grows vigorously, exhibits strong resistance to heat and root-knot nematode disease, and blooms profusely, with many vines bearing flowers at nearly every node. Most vines have two flowers per peduncle, and some even have three, compared to the single flower per peduncle of most cultivated Momordica grosvenori varieties. Winged Momordica grosvenori fruits are large, round, and uniform, with a higher rate of large fruit than Momordica grosvenori. Winged Momordica grosvenori contains 28% sugar (calculated as glucose), higher than the 26% found in the green-skinned fruit of Momordica grosvenori, making it an ideal parent for breeding new high-yielding, heat-resistant, and root-knot nematode-resistant Momordica grosvenori varieties. However, the wild germplasm resources of Winged Momordica grosvenori are endangered. Although discovered and reported in the 1990s, they have not been effectively developed and utilized to date due to material loss and the lack of breakthroughs in seedling propagation.

[0004] Conventional Momordica grosvenori cuttings are prone to premature wilting due to water loss, resulting in a low survival rate (see the applicant's prior patent application publication number CN 102668854A). However, during the Guangxi medicinal resource survey and research, the applicant's research group introduced and domesticated wild plants of Pterocarpus grosvenori and found that it has the following characteristics different from Momordica grosvenori and directly affects the rapid propagation of seedlings: (1) In the seed propagation research, directly taking plant branches for cuttings has an extremely low survival rate or no survival, and the cuttings are prone to mildew. Taking the growing apical buds or axillary buds for tissue culture also has the problem of material mildew and failure to survive; (2) The leaves grow vigorously, the vines have long internodes, and there is a phenomenon of excessive growth. In order to solve the problem of the difficulty of rapid propagation of Pterocarpus grosvenori seedlings, the present invention was studied. Summary of the Invention

[0005] In view of the above problems, the purpose of the present invention is to provide a micro-cutting seedling method for winged Momordica grosvenori. According to the growth characteristics of winged Momordica grosvenori, a stem control and root inducing agent and an antiseptic and rooting agent are developed. The stem control and root inducing agent are used to regulate the growth of stem nodes, and the stem nodes are induced to root by light and dark control treatment. The treated stem nodes are wrapped with yellow mud slurry of the antiseptic and rooting agent and then cut. Seedlings are obtained by subsequent light control, moisture control, and stem control and root inducing agent spraying culture, thereby solving the problems of winged Momordica grosvenori cuttings not taking root or having difficulty in taking root, low survival rate, and difficulty in rapid propagation of seedlings.

[0006] To solve the above problems, the present invention provides a micro-cutting seedling raising method for Momordica grosvenori, comprising:

[0007] Step S1: Place the vine on loose ground for cultivation, and use stem control and root inducer to regulate the growth of the mother plant's stem nodes; the stem control and root inducer can make the mother plant's stem nodes shorter and relatively thicker, which is the basis for high survival rate of cuttings, and the number of short stem nodes is greater, so more cutting materials can be obtained.

[0008] Step S2: Dark-treat the middle and rear end vines of the mother plant to obtain pre-treated vines; dark treatment can inhibit the excessive growth of stems and leaves. After dark treatment, the rooting tendency of the stem nodes increases, paving the way for the subsequent rooting of cuttings, which is a very critical step for the rooting and survival of cuttings.

[0009] Step S3: taking stem segments with one leaf per two nodes or one leaf per one node from the pretreated vine as cutting materials, and only keeping one-third of the leaves; preferably, two leaves per two nodes is a better cutting material, which can improve the survival rate of cuttings.

[0010] Step S4: Dipping the base of the cutting material with yellow mud, an antiseptic and root-growing agent, to obtain mud-coated cuttings;

[0011] Step S5: implanting or burying the mud-wrapped cuttings into a substrate in April, June, August or October of each year;

[0012] Step S6: Cultivate in an environment with a light transmittance of 10%-15%, a temperature of 25-30°C, and a relative humidity of 80-90%. When the second leaf of the new bud unfolds, spray with an aqueous solution of a stem control and root inducer, once every 5-7 days until transplanting.

[0013] Among them, each kilogram of antiseptic rooting agent yellow mud slurry contains: Lingfasu 0.3-0.5mg, sodium naphthaleneacetate 300mg, potassium indolebutyrate 500mg, moisturizer 100mg, Zhongshengmycin 30-35mg, thiophanate-copper 320-400mg, sodium nitrophenolate 3.6mg and yellow mud powder 600-800g;

[0014] Each kilogram of stem control and root inducer contains: 0.3-0.5 mg of hemichlorothiazolin, 25 mg of spermidine, 10-20 mg of pyrimidine alcohol, 100-150 mg of calcium cyclohexadione, 100 mg of mineral fulvic acid, 1000 mg of potassium dihydrogen phosphate and 800 mg of organosilicon.

[0015] Preferably, in steps S1 and S2, from March to August, the vines of the climbing mother plant are laid flat on the ground for cultivation, with the growing buds placed in the same direction, and moist fine river sand or loose soil is sprinkled around the vine nodes; the growing buds and leaves of the plant are sprayed with an aqueous solution of a stem control and root inducer 5-6 times, once every 5-6 days, and cultivated for 20 to 25 days; then the front section of the vine 60-100 cm long from the growth point is coiled and bent to press the seedlings, laid flat on the bed surface, and covered with an opaque cover for 5-6 days for dark treatment to obtain pretreated vines.

[0016] Preferably, in the micro-cutting seedling raising method of Momordica grosvenori, the stem segment selected in step S3 is a stem segment with two nodes and one leaf.

[0017] Preferably, in the micro-cutting seedling raising method for Momordica grosvenori, the cutting time in step S5 is June.

[0018] Preferably, in the micro-cutting seedling method of the winged Momordica grosvenori, each kilogram of the antiseptic and rooting agent yellow mud slurry contains: 0.4 mg of lingfasu, 300 mg of sodium naphthaleneacetate, 500 mg of potassium indolebutyrate, 100 mg of moisturizer, 33 mg of zhongshengmycin, 360 mg of thiophanate-copper, 3.6 mg of sodium nitrophenolate, and 700 g of yellow mud powder; the raw materials are added to distilled water to 1000 ml and mixed evenly to obtain the antiseptic and rooting agent yellow mud slurry.

[0019] Preferably, in the micro-cutting seedling raising method of Siraitia grosvenori, the aqueous solution of the stem control and root inducing agent contains: 0.4 mg of hemichlorothiazolin, 25 mg of spermidine, 15 mg of pyrimidine alcohol, 125 mg of prohexadione calcium, 100 mg of mineral fulvic acid, 1000 mg of potassium dihydrogen phosphate, and 800 mg of organosilicon; the raw materials are added to 1000 ml of distilled water and mixed evenly to obtain the aqueous solution of the stem control and root inducing agent.

[0020] Preferably, in the micro-cutting seedling raising method of Siraitia grosvenori, step S4 is to dip the base of the cutting material into yellow mud slurry, which is an antiseptic and rooting agent, and then air it in the shade to form a mud coating layer, and then repeat the process until the thickness of the mud coating layer reaches 1 to 7 mm.

[0021] Preferably, in the micro-cutting seedling raising method of Siraitia grosvenori, in step S4, the base of the cutting material is dipped in yellow mud slurry as an antiseptic and rooting agent, then in wood ash, and then in yellow mud slurry as an antiseptic and rooting agent to form a mud coating layer, and the thickness of the mud coating layer is 6-7 mm.

[0022] Preferably, in the micro-cutting seedling raising method of Siraitia grosvenori, the matrix is ​​prepared by cutting dry pine needles into segments of about 0.5 cm, and mixing vermiculite: fine river sand: pine needles: peat soil in a volume ratio of 3:2:1:1 to prepare the matrix.

[0023] The present invention has at least the following beneficial effects:

[0024] 1. The present invention develops a stem-controlling root-inducing agent and an antiseptic rooting agent based on the growth characteristics of the winged Momordica grosvenori. The stem-promoting root-inducing agent is used to regulate the growth of the stem nodes, and the light and dark treatment is used to induce the stem nodes to root. The treated stem nodes are wrapped with yellow mud slurry of the antiseptic rooting agent and then cut. After subsequent light and humidity control, and spray cultivation with the stem-control root-inducing agent, seedlings are obtained. This method uses less materials, has a high material utilization rate, is simple to operate, and has a high survival rate. It can be bred from March to October, breaking the limitation that seedlings can only be raised by pressing potatoes in autumn, and can solve the problem of low survival rate of winged Momordica grosvenori cuttings.

[0025] 2. In the aqueous solution of the stem control and root inducing agent of the present invention, the mitogen and the growth inhibitor are combined, which can not only inhibit the rapid growth of leaves and stem nodes and prevent excessive growth, but also promote the rooting of stem nodes. The rapid division, growth and renewal of the root system can resist mold, and the growth of the root system can promote the absorption of nutrients and improve the survival rate.

[0026] 3. The antiseptic rooting agent yellow mud slurry of the present invention uses a combination of zhongshengmycin, thiophanate-methyl and sodium nitrate for sterilization, which is efficient and long-lasting; it uses a combination of lingfasu, sodium naphthaleneacetate, potassium indolebutyrate and sodium nitrate to promote rooting, promote rapid growth of the root system, help the cuttings survive the mildew period, and improve the survival rate.

[0027] 4. The present invention explores the effect of the thickness of the yellow mud coating on the seedling establishment time and survival rate of the winged monk fruit. When the yellow mud is used alone to wrap the root base, the coating thickness is about 3 mm, and the seedling establishment time and survival rate are the best. In one embodiment, a 6-7 mm coating layer is obtained by combining yellow mud with plant ash. The coating has good permeability and can maintain the concentration of the base agent for a long time to exert its effect, which can significantly improve the survival rate.

[0028] 5. The present invention selects to carry out cuttings in June, taking advantage of the heat resistance and vigorous growth characteristics of the winged monk fruit to resist mold and survive the cutting mildew period, thereby improving the survival rate and shortening the seedling time. Using two-node one-leaf stem segments as cutting materials, the two-node stem segments have good nutrient reserves and can better cope with the excessive growth characteristics and meet the nutrient requirements for rooting and sprouting. During the cultivation process, a dark, temperature-controlled and humidity-controlled environment with a light transmittance of 10-20%, a temperature of 25-30°C, and a relative humidity of 80-90% is used to induce rooting and sprouting, inhibit excessive growth of the stem nodes, and improve the survival rate.

[0029] 6. The method of the present invention uses a stem control and root inducing agent to pretreat the mother plant, adjust the vine internode length, use seedling pressing and light and dark control treatment to induce rooting, and obtain pretreated vines, which effectively inhibits the excessive growth of the winged Momordica grosvenori stem nodes and promotes the rooting of subsequent vine stem node cuttings.

[0030] Other advantages, objectives and features of the present invention will be reflected in part from the following description and will be understood by those skilled in the art through study and practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is a diagram of rooting of cuttings after cutting in Example 1;

[0032] Figure 2 The winged Momordica grosvenori seedlings obtained by culture in Example 1;

[0033] Figure 3 Comparison of internode length between pretreated and unpretreated vines. DETAILED DESCRIPTION

[0034] The present invention is further described in detail below with reference to the embodiments so that those skilled in the art can implement the invention with reference to the description.

[0035] The process of preparing the yellow mud slurry of the antiseptic and root-inducing agent is as follows: weighing (taking) substances containing the following mass (calculated by effective ingredients): 0.3-0.5 mg of lingfasu, 300 mg of sodium naphthaleneacetate, 500 mg of potassium indolebutyrate, 100 mg of moisturizing agent, 30-35 mg of zhongshengmycin, 320-400 mg of thiophanate-methyl (longkebacillus), 3.6 mg of sodium nitrophenolate, and 600-800 g of yellow mud powder; adding distilled water to 1000 ml to obtain an aqueous solution of the stem-promoting and root-inducing agent for standby use.

[0036] The process of preparing the aqueous solution of the stem control and root inducer is as follows: weighing (taking) substances containing the following mass (calculated as active ingredients): 0.3-0.5 mg of hemichlorothiazolin, 25 mg of spermidine, 10-20 mg of pyrimidine alcohol (A-Rest), 100-150 mg of prohexadione calcium, 100 mg of mineral-derived fulvic acid, 1000 mg of potassium dihydrogen phosphate, and 800 mg of organosilicon, mixing the mixture, and adding distilled water to 1000 ml to obtain the aqueous solution of the stem control and root inducer for standby use.

[0037] Example 1

[0038] (1) Pretreatment of the mother plant

[0039] The test materials were plants introduced from Napo County, Baise City, Guangxi, and have been domesticated and cultivated for three years in the Guangxi Zhuang Autonomous Region Medicinal Plant Garden. The experiment began in 2022 in a seedling greenhouse at the Guangxi Zhuang Autonomous Region Medicinal Plant Garden Research Base in Xingning District, Nanning City, Guangxi.

[0040] From March to October, lay the climbing vines of the mother plant flat on a loose bed with the growing buds oriented in the same direction. Sprinkle moistened fine river sand or loose soil around the vine nodes. Spray the growing buds and leaves with a stem control and root inducer 4-5 times, every 5-6 days, for 20-25 days.

[0041] Then, coil and bend the front section of the vine 60-100cm away from the growth point and cover it with a light-proof wooden basin, foam basin or plastic basin 3-5cm high and 50-100cm in diameter for 5-6 days for dark treatment. Figure 3 The vine on the left has internodes about 4 cm long.

[0042] (2) Cutting material processing

[0043] Take the vines that have been treated as above and cut them into stem segments 4-6 cm long with 2 nodes and one leaf or 1 node and one leaf as cutting materials. Only keep one-third of the leaves and cut off the rest.

[0044] (3) Anti-corrosion and root growth promotion measures for cutting materials

[0045] Dip the base of the cuttings in yellow mud slurry, which is an antiseptic and root-growing agent, and air it in the shade for 5-10 minutes to obtain mud-coated cuttings. The thickness of the mud layer formed by the yellow mud slurry is about 1 mm.

[0046] Preparation of yellow mud slurry as an antiseptic and root-inducing agent: Lingfasu 0.4 mg, sodium naphthaleneacetate 300 mg, potassium indolebutyrate 500 mg, moisturizer 100 mg, zhongshengmycin 33 mg, thiophanate-methyl (Lonconazole) 360 mg, sodium nitrophenolate 3.6 mg, yellow mud powder (sterilized by baking at above 100°C for 30 minutes) 700 g, add distilled water to 1000 ml, mix evenly to obtain yellow mud slurry for promoting stems and inducing roots.

[0047] (4) Cutting

[0048] In April, June, August, and October, the mud-wrapped cuttings were inserted or buried in the seedbed substrate, exposing the petioles and leaves. The substrate was prepared by cutting dry pine needles into approximately 0.5 cm segments and mixing vermiculite, fine river sand, pine needles, and peat soil in a ratio of 3:2:1:1 by volume. The substrate was sprayed with distilled water to moisten it (to a moisture content of approximately 25%), sealed in a plastic bag, and exposed to sunlight for 4-5 days. The substrate was then spread in the seedbed to a thickness of 10-12 cm.

[0049] (5) Cultivation

[0050] Cultivate in an environment with a light transmittance of 10%-15%, a temperature of 25-30°C, and a relative humidity of 80-90%. When new shoots have one or two expanded leaves, spray with an aqueous solution of a stem control and root inducer every 5-7 days. When seedlings are 12-15 cm long and have 4-5 nodes, they can be transplanted.

[0051] The aqueous solution of stem control and root inducer is prepared as follows: weigh (measure) the following substances by mass (calculated as active ingredients): 0.4 mg of hemichlorothiazolin, 25 mg of spermidine, 15 mg of pyrimidine alcohol (A-Rest), 125 mg of calcium cyclamate, 100 mg of mineral fulvic acid, 1000 mg of potassium dihydrogen phosphate, and 800 mg of silicone, mix them, and add distilled water to 1000 ml to obtain the aqueous solution of stem control and root inducer.

[0052] Example 2

[0053] The difference from Example 1 is that in step (3), the base of the cuttings is dipped in yellow mud slurry, an antiseptic and root-growing agent, and aired in the shade for 5-10 minutes, and repeated once to obtain two layers of mud-coated cuttings, with a thickness of about 2-3 mm.

[0054] Example 3

[0055] The difference from Example 1 is that in step (3), the base of the cuttings is dipped in yellow mud slurry, an antiseptic and root-growing agent, and aired in the shade for 5-10 minutes, and repeated twice to obtain three layers of mud-coated cuttings with a total thickness of about 4-5 mm.

[0056] Example 4

[0057] The difference from Example 1 is that in step (3), the base of the cuttings is dipped in yellow mud slurry, an antiseptic and root-growing agent, and aired in the shade for 5-10 minutes, and repeated three times to obtain four layers of mud-coated cuttings with a total thickness of about 6-7 mm.

[0058] Example 5

[0059] The difference from Example 1 is that in step (3), the base of the cutting is dipped in yellow mud slurry, which is an antiseptic and root-growing agent, then dipped in a layer of wood ash, and then dipped in yellow mud slurry, which is an antiseptic and root-growing agent, to obtain a mud-wrapped cutting with two layers of yellow mud and one layer of wood ash, and the total thickness of the mud-wrapped layer is about 6-7 mm.

[0060] Comparative Example 1

[0061] The difference from Example 1 is that in the anti-corrosion and root-growth-promoting measures of the cutting materials in step (3), the anti-corrosion and root-growth agent yellow mud slurry does not contain sodium naphthaleneacetate and potassium indolebutyrate, and only contains 0.4 mg of lingfasu, 100 mg of moisturizer, 33 mg of zhongshengmycin, 360 mg of thiophanate-methyl (Lonconazole), 3.6 mg of sodium nitrophenolate, and 700 g of yellow mud powder (sterilized by baking at above 100°C for 30 minutes), and the rest are the same.

[0062] Comparative Example 2

[0063] The difference from Example 1 is that in the anti-corrosion and root-growth-promoting measures of the cutting materials in step (3), the anti-corrosion and root-growth agent yellow mud slurry does not contain lingfasu, but only contains 300 mg of sodium naphthaleneacetate, 500 mg of potassium indolebutyrate, 100 mg of moisturizer, 33 mg of zhongshengmycin, 360 mg of thiophanate-methyl (Lonconazole), 3.6 mg of sodium nitrophenolate, and 700 g of yellow mud powder (sterilized by baking at above 100°C for 30 minutes), and the rest are the same.

[0064] Comparative Example 3

[0065] The difference from Example 1 is that in the anti-corrosion and rooting promotion measures of the cutting materials in step (3), the anti-corrosion and rooting agent yellow mud slurry does not contain zhongshengmycin, but only contains 0.4 mg of lingfasu, 300 mg of sodium naphthaleneacetate, 500 mg of potassium indolebutyrate, 100 mg of moisturizer, 360 mg of thiophanate-methyl (Lonconazole), 3.6 mg of sodium nitrophenolate, and 700 g of yellow mud powder (sterilized by baking at above 100°C for 30 minutes), and the rest are the same.

[0066] Comparative Example 4

[0067] The difference from Example 1 is that in the anti-corrosion and rooting promotion measures of the cutting materials in step (3), the anti-corrosion and rooting agent yellow mud slurry does not contain thiophanate-methyl (Lonconazole), and only contains 0.4 mg of lingfasu, 300 mg of sodium naphthaleneacetate, 500 mg of potassium indolebutyrate, 100 mg of moisturizer, 33 mg of zhongshengmycin, 3.6 mg of sodium nitrophenolate, and 700 g of yellow mud powder (sterilized by baking at above 100°C for 30 minutes), and the rest are the same.

[0068] Comparative Example 5

[0069] The difference from Example 1 is that in step (5), the aqueous solution of the stem control and root inducer does not contain growth inhibitors, but only contains: 0.4 mg of hemichlorothiazolin, 25 mg of spermidine, 100 mg of mineral fulvic acid, 1000 mg of potassium dihydrogen phosphate, and 800 mg of silicone, which are mixed and distilled water is added to 1000 ml to obtain the aqueous solution of the stem control and root inducer.

[0070] Comparative Example 6

[0071] The difference from Example 1 is that in step (5), the aqueous solution of the stem control and root inducer does not contain a mitogen, but only contains 15 mg of pyrimidine alcohol (A-Rest), 125 mg of calcium prohexadione, 100 mg of mineral fulvic acid, 1000 mg of potassium dihydrogen phosphate, and 800 mg of organosilicon, which are mixed and distilled water is added to 1000 ml to obtain an aqueous solution of the stem control and root inducer.

[0072] Comparative Example 7

[0073] The difference from Example 1 is that in step (5) culturing, an environment with a light transmittance of 80-90% is used, and the other aspects are the same.

[0074] Comparative Example 8

[0075] The difference from Example 1 is that in step (1), the mother plant is not pretreated, but the normally elongated vines of the mother plant are cut into cutting materials, and the other parts are the same. Figure 3 The vine on the middle right has internodes up to 15 cm long in a normal vine without pretreatment.

[0076] Effect analysis

[0077] 1. Effects of cutting time and cutting materials on the survival rate of Momordica grosvenori cuttings

[0078] The emergence time and survival rate of different cutting times and node numbers in Example 1 were statistically analyzed in detail. The results are shown in Table 1 below:

[0079] Table 1 Effects of different seedling emergence and cutting time and node number on seedling emergence time and survival rate

[0080]

[0081] The results show that:

[0082] (1) The survival rate of cuttings of Winged Momordica grosvenori varied at different cutting times. Under the same cutting material conditions, the highest survival rate was achieved in June. The survival rate of cuttings with one node and one leaf was 87%, and that with two nodes and one leaf reached 91%. The survival rate of cuttings taken in August was lower, with the survival rate of cuttings with one node and one leaf being 83%. Compared with cuttings with one node and one leaf, the emergence time of cuttings with two nodes and one leaf was faster and shorter. The emergence time of cuttings with two nodes and one leaf in June was shorter than that in other months, which could significantly shorten the seedling raising cycle and reduce time costs.

[0083] (2) Different cutting materials also have a certain impact on their survival rate. Under the same cutting time, the survival rate of cuttings with two nodes and one leaf is higher, reaching the highest 91%.

[0084] The data in Table 1 above show that the method of the present invention can promote the germination and rooting of the cuttings of Pteris grosvenori to form new plants. The seedling emergence speed is fast, and new shoots can grow in about 30 days. The survival rate is high. This method uses less materials, is simple to operate, and has a high survival rate. It can solve the problem of low survival rate of Pteris grosvenori cuttings.

[0085] 2. Effect of Mud Wrapping at the Base of Cuttings on the Survival Rate of Momordica grosvenori Cuttings

[0086] The effects of different mud-wrapped cuttings on the emergence time and survival rate in Examples 1 to 5 were statistically analyzed in detail. The results are shown in Table 2 below:

[0087] Table 2 Effects of different mud-wrapped cuttings on emergence time and survival rate

[0088]

[0089] As can be seen from the above table, as the thickness of the yellow mud layer increases, the emergence time of the winged Momordica grosvenori is on the rise, and the survival rate is a trend of first rising and then falling, indicating that a yellow mud layer of appropriate thickness can improve the survival rate, but too thick yellow mud reduces the survival rate, which may be related to the excessively thick yellow mud hindering the absorption of water and nutrients by the cuttings. In Example 5, yellow mud is used in combination with a mud layer of plant ash, and the mud layer reaches 6-7 mm, but the emergence time is similar to that of Examples 1 and 2, and the survival rate is greatly improved, up to 98%, which may be related to the plant ash mud layer having a longer maintenance effect of the agent and having permeability. Repeatedly dipping in yellow mud and drying in the shade during the production process requires more process steps and time. Taking into account the manpower and time cost of production and the survival rate, the schemes of Example 2 or Example 5 can be used. In the scheme of Example 5, dipping in yellow mud in combination with plant ash is used, without waiting for drying in the shade, and the survival rate is high.

[0090] 3. Effects of yellow mud components of the antiseptic rooting agent on the survival rate of Momordica grosvenori cuttings

[0091] The effects of yellow mud slurry, an antiseptic and root-growing agent, on the emergence time and survival rate of Momordica grosvenori in Example 1 and Comparative Examples 1-4 were statistically analyzed in detail. The cutting time was June. The results are shown in Table 3 below:

[0092] Table 3 Effects of yellow mud slurry, an antiseptic and rooting agent, on the emergence time and survival rate of Momordica grosvenori

[0093]

[0094] As can be seen from the table above, Lingfasu, sodium naphthaleneacetate, potassium indolebutyrate, Zhongshengmycin, and thiophanate-copper (Longke Bacteria) all had a significant effect on the survival rate of Winged Momordica grosvenori. The combination of Lingfasu, sodium naphthaleneacetate, and potassium indolebutyrate significantly shortened the emergence time and increased the survival rate. The combination of thiophanate-copper, thiophanate-copper, and sodium nitrate was highly effective in sterilizing and improving the survival rate, but had little effect on the emergence time.

[0095] 4. Effects of stem control and root inducer components on the survival rate of Momordica grosvenori cuttings

[0096] The effects of the aqueous solution of the stem control and root inducing agent on the emergence time and survival rate of Momordica grosvenori in Example 1 and Comparative Examples 5-6 were statistically analyzed in detail. The cutting time was June. The results are shown in Table 4 below:

[0097] Table 4 Effects of aqueous solution of stem control and root inducer on emergence time and survival rate of Momordica grosvenori

[0098]

[0099] As can be seen from the table above, the combination of mitogens and growth inhibitors, such as hemichloroquine, spermidine, pyrimidinol (A-Rest), and prohexadione-calcium, in Example 1 significantly improved survival rates and shortened seedling emergence time. This may be due to the combined use of mitogens and growth inhibitors inhibiting excessive growth and promoting rooting. In contrast, the use of either mitogen or growth inhibitor alone in Comparative Examples 5 and 6 resulted in significantly decreased survival rates.

[0100] 5. Effect of light on the survival rate of Siraitia grosvenori cuttings during the cultivation process

[0101] The effects of culture illumination on the emergence time and survival rate of Momordica grosvenori in Example 1 and Comparative Example 7 were statistically analyzed in detail. The cutting time was June. The results are shown in Table 5 below:

[0102] Table 5 Effect of culture transmittance on the emergence time and survival rate of Momordica grosvenori

[0103]

[0104] As can be seen from the above table, Example 1 uses a dark environment with a transmittance of 10%-15% for dark treatment culture, and the survival rate reaches 91%. Comparative Example 7 uses a light condition with a transmittance of 80%-90% for culture, and the survival rate is only 53%. The difference in survival rates between the two may be related to the excessive growth characteristics of the winged Momordica grosvenori. The dark environment combined with temperature and humidity control can inhibit the excessive growth of the nodes and leaves of the winged Momordica grosvenori cuttings and induce rooting of the stem nodes. Under brighter light conditions, rooting of the stem nodes of the winged Momordica grosvenori vines is more difficult and the survival rate is not high.

[0105] 6. Effect of Pretreatment of Vine Stem Control on Survival Rate

[0106] The effects of the stem control pretreatment of the mother plant vines on the emergence time and survival rate of Momordica grosvenori in Example 1 and Comparative Example 8 were statistically analyzed in detail. The cutting time was June. The results are shown in Table 6 below:

[0107] Table 6 Effects of vine stem control pretreatment on the emergence time and survival rate of Momordica grosvenori

[0108]

[0109]

[0110] From the above table and Figure 3 It can be seen that the internode length of the pretreated vine is about 4 cm, and the internode length of the normal vine without pretreatment is about 15 cm. After the pretreated vines are made into cutting materials for seedling cultivation, the survival rate is as high as 91%, while after the untreated vines are made into cutting materials for seedling cultivation, the survival rate is only 49%. The reason may be related to the excessive growth of the stems and leaves of the winged Momordica grosvenori. Example 1 uses an aqueous solution of a stem control and root inducer to regulate the mother plant, and the vine internode length becomes shorter. Combined with the vine coiling, bending and pressing seedling treatment, and the dark cover treatment, the excessive growth phenomenon is suppressed, and it is easier to induce the stem nodes to take root. In contrast, the vines in Comparative Example 8 are excessively long, and the stem nodes take root less or even do not take root.

[0111] Although the embodiments of the present invention have been disclosed above, they are not limited to the applications listed in the specification and embodiment. They can be applied to various fields suitable for the present invention. For those familiar with the art, additional modifications can be easily realized.

Claims

1. A micro-cutting seedling raising method for Momordica grosvenori, characterized in that: include: Step S1: placing the vine on loose ground for cultivation, and using a stem control and root inducer to regulate the growth of the stem nodes of the mother plant; Step S2: dark-treating the middle and rear end vines of the mother plant to obtain pre-treated vines; Step S3: taking a stem segment with two nodes and one leaf from the pre-treated vine as cutting material; and only retaining one-third of the leaf; Step S4: Dipping the base of the cutting material with yellow mud, an antiseptic and root-growing agent, to obtain mud-coated cuttings; Step S5: in June of each year, the mud-wrapped cuttings are implanted or buried in the substrate; Step S6: Cultivate in an environment with a light transmittance of 10%-15%, a temperature of 25-30°C, and a relative humidity of 80-90%. When the second leaf of the new bud unfolds, spray with an aqueous solution of a stem control and root inducer, once every 5-7 days until transplanting. Among them, each kilogram of antiseptic rooting agent yellow mud slurry contains: Lingfasu 0.3-0.5mg, sodium naphthaleneacetate 300mg, potassium indolebutyrate 500mg, moisturizer 100mg, Zhongshengmycin 30-35mg, thiophanate-copper 320-400mg, sodium nitrophenolate 3.6mg and yellow mud powder 600-800g; Each kilogram of stem control and root inducer contains: 0.3-0.5 mg of hemichlorothiazolinone, 25 mg of spermidine, 10-20 mg of pyrimidine alcohol, 100-150 mg of prohexadione calcium, 100 mg of mineral fulvic acid, 1000 mg of potassium dihydrogen phosphate and 800 mg of organosilicon. In steps S1 and S2, from March to August, the climbing vines of the mother plant are laid flat on loose ground for cultivation, with the growing buds arranged in the same direction, and moist fine river sand or loose soil is sprinkled around the vine nodes; the growing buds and leaves of the plant are sprayed with an aqueous solution of a stem control and root inducer 5-6 times, once every 5-6 days, for 20-25 days; Then, the front section of the vine 60-100 cm away from the growth point is coiled and bent, laid flat on the bed surface, and covered with an opaque cover for 5-6 days for dark treatment to obtain pretreated vines; Dip the base of the cutting material in yellow mud as an antiseptic and rooting agent, then dip it in wood ash, and then dip it in yellow mud as an antiseptic and rooting agent again to form a mud coating layer with a thickness of 6-7 mm.

2. The micro-cutting seedling raising method of Momordica grosvenori according to claim 1, wherein: Each kilogram of the antiseptic and root-growing agent yellow mud slurry contains: 0.4 mg of lingfasu, 300 mg of sodium naphthaleneacetate, 500 mg of potassium indolebutyrate, 100 mg of moisturizer, 33 mg of zhongshengmycin, 360 mg of thiophanate-copper, 3.6 mg of sodium nitrophenolate, and 700 g of yellow mud powder; the raw materials are added to distilled water to 1000 ml, and mixed evenly to obtain the antiseptic and root-growing agent yellow mud slurry.

3. The micro-cutting seedling raising method of Momordica grosvenori according to claim 1, wherein: The aqueous solution of the stem control and root inducing agent contains: 0.4 mg of hemichlorothiazolin, 25 mg of spermidine, 15 mg of pyrimidine alcohol, 125 mg of prohexadione calcium, 100 mg of mineral fulvic acid, 1000 mg of potassium dihydrogen phosphate, and 800 mg of organosilicon; the raw materials are added to distilled water to 1000 ml, and mixed evenly to obtain the aqueous solution of the stem control and root inducing agent.

4. The micro-cutting seedling raising method of Momordica grosvenori according to claim 1, wherein: Dip the base of the cutting material in yellow mud, an antiseptic and rooting agent, and dry it in the shade to form a mud layer. Repeat this process until the thickness of the mud layer reaches 1 to 7 mm.

5. The micro-cutting seedling raising method of Momordica grosvenori according to claim 1, wherein: The matrix was prepared by cutting dry pine needles into 0.5 cm segments and mixing vermiculite, fine river sand, pine needles, and peat soil in a volume ratio of 3:2:1:1 to prepare the matrix.

Images