An attractant for smooth-hipped eight-toothed beetles, a lure core, and a preparation method and application thereof

Abstract

The present invention provides an attractant, an attractant core, a preparation method and an application of a light-hipped eight-toothed beetle, and belongs to the field of light-hipped eight-toothed beetle control and its green ecological prevention and control technology. The attractant includes the following components in parts by weight: 4 parts of 2-methyl-3-butene-2-ol, 0.04 parts of (±)-dienol, 0.02 parts of (S)-cis-verbenol and 0.2 parts of trans-2-hexenal. The attractant provided by the invention has an excellent luring effect on light-hipped eight-toothed beetles. Compared with conventional light-hipped eight-toothed beetle aggregation pheromone attractants, its trapping amount can be increased by 3 to 4 times, and it has the advantages of strong specificity, long lasting effect, and environmental friendliness. It is suitable for long-term monitoring and efficient prevention and control of light-hipped eight-toothed beetle populations, and is not affected by terrain and topography.

Description

Technical Field

[0001] The invention relates to an attractant for a sphenodon glabrata beetle, an attractant core, a preparation method and an application thereof, and belongs to the technical field of sphenodon glabrata beetle prevention and control and green ecological prevention and control. Background Art

[0002] Ips nitidus Eggers belongs to the genus Ips of the family Curculionidae. It is one of the main trunk-boring pests in natural spruce (Picea spp.) forests in western plateau areas of my country, such as Qinghai Province. It can harm many spruce species, including Picea crassifolia Kom., P. purpurea Mast., P. likiangensis var. rubescens Rehder & E.H. Wilson, and is most harmful to Picea crassifolia Kom. and P. purpurea Mast., which are endemic to Qinghai. This bark beetle is a secondary pest that mainly harms weak spruce trees such as wind-fallen trees, wind-broken trees, weak trees (wood damaged by natural disasters, mechanical damage) and dying trees in spruce forests. When the population density is high, it will also directly attack healthy standing trees. It often occurs together with the Shangri-La toothed bark beetle Ips shangrila Cognato & Sun and the Oriental toothed bark beetle Pseudipsorientalis Wood & Yin, causing massive death of trees in the forest.

[0003] At present, the prevention and control of tooth beetle pests mainly relies on the use of aggregation pheromones to trap and kill, assisted by manual cleaning of damaged trees. In view of the similarities between the morphological characteristics and phylogeny of the spruce eight-toothed beetle Ips typographus Linnaeus and the smooth-hipped eight-toothed beetle, it was initially mistakenly believed that the tooth beetles distributed in the spruce forests of Qinghai Province were spruce eight-toothed beetles, so attempts were made to use the aggregation pheromone of spruce eight-toothed beetles to lure and monitor the adults of smooth-hipped eight-toothed beetles. However, because these two tooth beetles are independent natural species, the insect trapping effect was not ideal. Later, the forestry department of Qinghai Province also used the known aggregation pheromone components of the genus Ips to screen potential aggregation pheromone attractants for smooth-hipped eight-toothed beetles in field experiments in various combinations, but no substantial breakthroughs were made. In 2009, Zhang Qinghe and his colleagues used solvent extraction and chemical analysis of the male hindgut to identify the aggregation pheromone of the smooth-hipped eight-toothed beetle for the first time in the world, and proposed the aggregation pheromone attractant components for luring and killing the smooth-hipped eight-toothed beetle, which are mainly: 65-97% 2-methyl-3-butene-2-ol, 2-20% (±) verbenadenol and 1-10% (S)-cis-verbenol, which are helpful for long-term monitoring of the smooth-hipped eight-toothed beetle population and efficient trapping and prevention and control.

[0004] In the process of the smooth-hipped eight-toothed beetle invading and harming host plants, its invasion behavior is not only affected by the pheromones produced by itself, but also by the volatiles from plant sources, which are very important host location signal recognition substances. The smooth-hipped eight-toothed beetle uses the differences in the composition of volatile substances released by healthy and weak trees to identify and select host trees with weaker resistance to attack and eventually successfully colonize. Therefore, these plant volatiles can be used as behavioral regulators of the smooth-hipped eight-toothed beetle to increase the amount of bark beetles attracted or to repel bark beetles. At present, it has been found that some plant volatiles have a synergistic or inhibitory effect on the aggregation pheromone components of bark beetles, and such substances have the characteristics of wide sources, many types, environmental friendliness, and relatively low prices. Therefore, using volatile components from hosts and non-hosts to regulate the behavior of the smooth-hipped eight-toothed beetle and adding plant volatiles as synergists or compounding agents to the aggregation pheromone components will help improve the insect-attracting efficiency of the aggregation pheromone of the smooth-hipped eight-toothed beetle, thereby achieving efficient ecological control function for the smooth-hipped eight-toothed beetle. Summary of the invention

[0005] In view of this, the object of the present invention is to provide an attractant for the smooth-hipped eight-toothed beetle, an attractant core, and a preparation method and application thereof. The attractant provided by the present invention can significantly improve the insect attracting effect of the aggregation pheromone of the smooth-hipped eight-toothed beetle, and achieve efficient attracting of the smooth-hipped eight-toothed beetle.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The present invention provides an attractant for the light-hipped eight-toothed beetle, comprising the following components in parts by weight:

[0008] 2-methyl-3-butene-2-ol, 4 parts;

[0009] (±)-dienol, 0.04 parts;

[0010] (S)-cis-verbenol, 0.02 parts;

[0011] Trans-2-hexenal, 0.2 parts.

[0012] The present invention also provides the use of the attractant described in the above technical solution in attracting the light-hipped eight-toothed beetle.

[0013] The present invention also provides an attractant core for attracting the smooth-hipped eight-toothed borer, comprising a slow-release carrier, absorbent cotton placed in the slow-release carrier, and the attractant described in the above technical solution adsorbed on the absorbent cotton.

[0014] Preferably, the sustained-release carrier is a polyethylene plastic bottle and a polyethylene plastic bag.

[0015] Preferably, in a single lure:

[0016] The content of 2-methyl-3-butene-2-ol is 4 g;

[0017] The content of (±)-dienol is 40 mg;

[0018] The content of (S)-cis-verbenol is 20 mg;

[0019] The content of trans-2-hexenal was 0.2 g.

[0020] Preferably, the content of cotton wool in a single lure is 5 g.

[0021] Preferably, the capacity of the polyethylene plastic bottle is 16 ml, and the bottle wall thickness is 0.6 mm; the thickness of the polyethylene plastic bag is 0.2 mm, the length is 150 mm, and the width is 80 mm.

[0022] The present invention also provides a method for preparing the lure core described in the above technical solution, comprising the following steps:

[0023] Place the absorbent cotton in a polyethylene plastic bottle, then drop the attractant on the absorbent cotton, and finally put it into a polyethylene plastic bag and seal it to obtain the attractant core.

[0024] The present invention also provides the use of the attractant core described in the above technical solution in attracting the smooth-hipped eight-toothed beetle.

[0025] Preferably, the application includes: using the lure core in conjunction with a cross-plate type bark beetle trap, the trap being set at the edge of a spruce forest or in an open, sunny, ventilated area within the forest, with a spacing of 30 to 50 m.

[0026] The beneficial effects of the present invention are:

[0027] The attractant provided by the present invention can significantly increase the insect attraction amount of the aggregation pheromone of the smooth-hipped eight-toothed beetle, achieve efficient attraction of the smooth-hipped eight-toothed beetle, and has the advantages of strong specificity, excellent attraction effect, long lasting effect, environmental friendliness, etc. It is suitable for long-term monitoring and efficient prevention and control of smooth-hipped eight-toothed beetle populations, and the attractant core is not affected by terrain and topography when used. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 The results of the biological activity evaluation test of the light-hipped eight-toothed beetle from May to June 2022 are, from left to right, Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, Comparative Example 5, Comparative Example 6 and Comparative Example 7;

[0029] Figure 2 The results of the test on the biological activity of the light-hipped eight-toothed beetle by varying the dosage of trans-2-hexenal in the attractant are shown in Example 2, Comparative Example 8, Comparative Example 9, Comparative Example 10 and Comparative Example 11 from left to right;

[0030] Figure 3 These are the results of a long-term synergistic evaluation test on the effect of the addition of trans-2-hexenal on the aggregation pheromone attractant of the light-hipped eight-toothed beetle (July 23 to September 1, 2023, Maixiu Forest Farm, Huangnan Tibetan Autonomous Prefecture, Qinghai Province). The line graphs are Example 3, Comparative Example 12 and Comparative Example 13 from top to bottom. DETAILED DESCRIPTION

[0031] The present invention provides an attractant for the light-hipped eight-toothed beetle, comprising the following components in parts by weight:

[0032] 2-methyl-3-butene-2-ol, 4 parts;

[0033] (±)-dienol, 0.04 parts;

[0034] (S)-cis-verbenol, 0.02 parts;

[0035] Trans-2-hexenal, 0.2 parts.

[0036] The present invention has no special limitation on the sources of the above reagents, and commercially available products can be used. In the present invention, the 2-methyl-3-butene-2-ol (McLean, purity>98%), (±)-beteldienoyl (B&K, purity>95%), (S)-cis-verbenol (Sigma-Aldrich, purity>95%), trans-2-hexenal (Aladdin, purity>97%).

[0037] The present invention also provides the use of the attractant described in the above technical solution in attracting the light-hipped eight-toothed beetle.

[0038] The present invention also provides an attractant core for attracting the light-hipped eight-toothed beetle, comprising a slow-release carrier, absorbent cotton placed inside the slow-release carrier, and the attractant described in the above technical solution adsorbed on the absorbent cotton. In the present invention, the slow-release carrier is preferably a polyethylene plastic bottle and a polyethylene plastic bag.

[0039] In the present invention, the content of the absorbent cotton in a single bait core is preferably 5g. In the present invention, the capacity of the polyethylene plastic bottle is preferably 16ml, and the bottle wall thickness is 0.6mm; the polyethylene plastic bag is preferably a black polyethylene plastic bag with a thickness of 0.2mm, a length of 150mm, and a width of 80mm. In the present invention, the content of 2-methyl-3-butene-2-ol in a single bait core is preferably 4g; the content of (±)-beetle dienol is preferably 40mg; the content of (S)-cis-verbenol is preferably 20mg; the content of trans-2-hexenal is preferably 0.2g. The present invention has no special requirements on the sources of the absorbent cotton, polyethylene plastic bottle and polyethylene plastic bag, and commercially available products can be used.

[0040] The present invention also provides a method for preparing the lure core described in the above technical solution, comprising the following steps:

[0041] Place the absorbent cotton in a polyethylene plastic bottle, then drop the attractant on the absorbent cotton, and finally put it into a polyethylene plastic bag and seal it to obtain the attractant core.

[0042] In the present invention, the preparation method of the attractant preferably includes: dissolving the (±)-dienol in 2-methyl-3-butene-2-ol in the required amount, and its concentration is preferably 40 mg / 4g; dissolving the (S)-cis-verbenol in 2-methyl-3-butene-2-ol in the required amount, and its concentration is preferably 20 mg / 4g; dissolving the trans-2-hexenal in 2-methyl-3-butene-2-ol in the required amount, and its concentration is preferably 0.2 g / 4g; mixing the four ingredients evenly and adding them to the absorbent cotton in the polyethylene plastic bottle carrier, and then putting them into a polyethylene plastic bag and sealing them to obtain the attractant.

[0043] The present invention also provides the use of the attractant core described in the above technical solution in attracting the smooth-hipped eight-toothed beetle.

[0044] The attractant core provided by the present invention can be used for luring and killing a large number of smooth-hipped eight-toothed beetles for prevention and control, or the attractant core can be used for long-term monitoring of smooth-hipped eight-toothed beetle populations. The attractant core provided by the present invention is easy to process and produce, with low cost, and the four components are stably released on the carrier respectively, and the effective period can be maintained for 2 months, which can meet the needs of luring a large number of smooth-hipped eight-toothed beetles in production. The attractant core provided by the present invention is an efficient, long-lasting, and environmentally friendly attractant core for smooth-hipped eight-toothed beetles, which is used in the luring and killing of a large number of smooth-hipped eight-toothed beetles for prevention and control, and has significant results. The attractant core for smooth-hipped eight-toothed beetles of the present invention has broad prospects for industrialization. In addition, its preparation method is simple, and it is particularly suitable for large-scale promotion in production.

[0045] In the present invention, the attractant core is used in conjunction with a cross-plate type bark beetle trap to attract the smooth-hipped eight-toothed bark beetle, and the trap is purchased from Beijing Zhongjie Sifang Biotechnology Co., Ltd. The large plate and the small plate are crossed to form a cross shape (500mm high and 300mm wide), connected to the upper cover through a hook at the top, and connected to the funnel through a hook at the bottom. The insect collecting bottle is directly connected to the trumpet mouth at the lower edge of the funnel. The attractant core is suspended in the middle position of the trap 20cm away from the top. The trap is fixed with two bamboo poles and iron wire, and is set at the edge of a spruce forest or in an open, sunny, and ventilated area in the forest, with a spacing of 30m to 50m, and the insect collecting bottle is about 50cm above the ground.

[0046] The technical solution provided by the present invention is described in detail below in conjunction with the forest biological activity test, but it is not intended to limit the protection scope of the present invention.

[0047] Example 1

[0048] The highly effective attractants for the smooth-hipped eight-toothed beetle prepared in this embodiment all use commercially available 2-methyl-3-butene-2-ol, whose chemical purity and isomeric purity are both greater than 98%; (±)-verbenol, whose chemical purity and isomeric purity are both greater than 95%; (S)-cis-verbenol, whose chemical purity and isomeric purity are both greater than 95%; and trans-2-hexenal, whose chemical purity and isomeric purity are both greater than 97%.

[0049] Highly effective attractant ingredients:

[0050] 2-methyl-3-butene-2-ol, 4 g;

[0051] (±)-dienol, 40 mg;

[0052] (S)-cis-verbenol, 20 mg;

[0053] Trans-2-hexenal, 200 mg.

[0054] The (±)-beetdienolide, (S)-cis-verbenol and trans-2-hexenal are directly added to 2-methyl-3-butene-2-ol according to the above ratio to obtain attractants with different ratios.

[0055] Preparation and storage of bait core: Add the attractant to 5g of cotton wool in a polyethylene plastic bottle carrier, then place it in a black polyethylene plastic bag and seal it.

[0056] The bait cores were sealed with aluminum foil and refrigerated at 4°C.

[0057] Comparative Example 1

[0058] The attractant is: 2-methyl-3-butene-2-ol 4g, (±)-dienol 40mg; the rest is the same as in Example 1, the results are shown in Figure 1 .

[0059] Comparative Example 2

[0060] The attractant is: 2-methyl-3-butene-2-ol 4g, (S)-cis-verbenol 20mg; the rest is the same as in Example 1, the results are shown in Figure 1 .

[0061] Comparative Example 3

[0062] The attractant is: 2-methyl-3-butene-2-ol 4g, trans-2-hexenal 200mg; the rest is the same as in Example 1, the results are shown in Figure 1 .

[0063] Comparative Example 4

[0064] The attractant is: 2-methyl-3-butene-2-ol 4g, (±)-dienol 40mg, trans-2-hexenal 200mg; the rest is the same as in Example 1, the results are shown in Figure 1 .

[0065] Comparative Example 5

[0066] The attractant is: 2-methyl-3-butene-2-ol 4g, (S)-cis-verbenol 20mg, trans-2-hexenal 200mg; the rest is the same as in Example 1, the results are shown in Figure 1 .

[0067] Comparative Example 6

[0068] The attractant is: 2-methyl-3-butene-2-ol 4g, (±)-beetle dienol 40mg, (S)-cis-verbenol 20mg; the rest is the same as in Example 1, the results are shown in Figure 1 .

[0069] Comparative Example 7

[0070] No attractants, only bait.

[0071] Comparative Examples 1 to 6 are prepared by directly adding a certain amount of (±)-dienol, (S)-cis-verbenol and trans-2-hexenal to 2-methyl-3-butene-2-ol. Comparative Example 7 is a trap with a blank bait core (consisting only of absorbent cotton, polyethylene plastic bottle and polyethylene plastic bag, without adding any other ingredients) as a blank control.

[0072] The attractant core of the light-hipped eight-toothed beetle attractant prepared by using Example 1 and Comparative Examples 1 to 7 is used to attract the light-hipped eight-toothed beetle, and the specific steps are as follows:

[0073] a) Production of the trap: The trap is mainly a cross-plate type bark beetle trap purchased from Beijing Zhongjie Sifang Biotechnology Co., Ltd. The large plate and the small plate are crossed to form a cross shape (500 mm high and 300 mm wide), connected to the upper cover through the hook at the top and connected to the funnel through the hook at the bottom. The trumpet connected to the insect collection bottle is placed on the lower part of the funnel and connected to the insect collection bottle.

[0074] b) Suspension of bait core: hang a bait core in the middle of the cross-plate bark beetle trap, 20 cm away from the top.

[0075] c) Field setting of traps: Three replicates were set for each experimental treatment. The traps were set at the edge of the spruce forest, and the distance between the traps was about 30m. Two 2m long bamboo poles with a diameter of about 5cm were inserted into the soil layer for about 50cm, and the distance between the two bamboo poles was 30cm. Then the trap was fixed between the two bamboo poles, and the insect collection bottle of the trap was about 50cm above the ground.

[0076] d) Data survey and trap emptying: In this experiment, each treatment was repeated three times, and the number of bark beetles trapped in each treatment was the average of the three traps. Depending on the weather conditions, the number of bark beetles in the insect collection bottle was observed every 2 to 3 days. After counting, the trapped bark beetles were placed in a plastic box and brought to the laboratory.

[0077] According to the above specific use steps, the application of attracting the light-hipped eight-toothed beetle. From May 20 to June 20, 2022, the trap with a blank lure core (Comparative Example 7) was used as a blank control. The specific results are shown in Figure 1 The distance between traps was about 30m, and the insect collecting bottle was about 50cm above the ground. In this experiment, each treatment was repeated three times, and the number of bark beetles trapped in each treatment was the average number of the three traps. Depending on the weather conditions, the number of bark beetles in the insect collecting bottle was observed every 2 to 3 days.

[0078] from Figure 1 The analysis of the insect attractant effect shows that compared with the high-efficiency attractant for Glechoma glabra (Example 1) and different attractants for Glechoma glabra (Comparative Examples 1, 2, 3, 4, 5, 6), Example 1 has the best trapping effect and the largest insect attractant amount, which is more than 3 times that of other attractant formulas. Comparative Example 3 and Comparative Example 7 (blank control) have no attractant activity for Glechoma glabra. This experiment further illustrates that the conventional aggregation pheromone attractant for Glechoma glabra (Comparative Example 6) significantly enhances the trapping efficiency for Glechoma glabra after adding trans-2-hexenal, and can significantly increase the trapping amount by 3 times, with a good attractant effect. The high-efficiency attractant for Glechoma glabra prepared using Example 1 can be used to comprehensively prevent and control Glechoma glabra in the forest and better protect spruce forests. The experimental conclusion confirmed that a mixture of 2-methyl-3-buten-2-ol, (±)-verbenol, (S)-cis-verbenol and trans-2-hexenal can kill a large number of O. glabra beetles in the forest. Among them, trans-2-hexenal is a highly effective synergist component of the conventional aggregation pheromone attractant core of O. glabra beetles. The mixture of the four constitutes an attractant with a strong attraction to O. glabra beetles, and none of them can be missing.

[0079] Example 2

[0080] The components of the highly effective attractant for the light-hipped eight-toothed beetle produced in this embodiment are as follows:

[0081] 2-methyl-3-butene-2-ol, 4 g;

[0082] (±)-dienol, 40 mg;

[0083] (S)-cis-verbenol, 20 mg;

[0084] Trans-2-hexenal, 200 mg.

[0085] The sample purity, lure preparation method, lure storage, and implementation steps are the same as those in Example 1.

[0086] Comparative Example 8

[0087] The attractant is: 2-methyl-3-butene-2-ol 4g, (±)-beetle dienol 40mg, (S)-cis-verbenol 20mg; trans-2-hexenal 20mg; the rest is the same as in Example 2, the results are shown in Figure 2 .

[0088] Comparative Example 9

[0089] The attractant is: 2-methyl-3-butene-2-ol 4g, (±)-dienol 40mg, (S)-cis-verbenol 20mg; trans-2-hexenal 400mg; the rest is the same as in Example 2, the results are shown in Figure 2 .

[0090] Comparative Example 10

[0091] The attractant is: 2-methyl-3-butene-2-ol 4g, (±)-beetle dienol 40mg, (S)-cis-verbenol 20mg; trans-2-hexenal 2g; the rest is the same as in Example 2, the results are shown in Figure 2 .

[0092] Comparative Example 11

[0093] No attractants, only bait.

[0094] Comparative Examples 8 to 10 are prepared by directly adding a certain amount of (±)-dienol, (S)-cis-verbenol and trans-2-hexenal to 2-methyl-3-butene-2-ol. Comparative Example 11 is a trap with a blank bait core (consisting only of sterile cotton wool, polyethylene plastic bottles and polyethylene plastic bags, without any added ingredients) as a blank control.

[0095] The attractant cores of the light-hipped eight-toothed beetle attractant prepared in Example 2 and Comparative Examples 8 to 11 are used to attract the light-hipped eight-toothed beetle, and the specific steps are as follows:

[0096] a) Production of the trap: The trap is mainly a cross-plate type bark beetle trap purchased from Beijing Zhongjie Sifang Biotechnology Co., Ltd. The large plate and the small plate are crossed to form a cross shape (500 mm high and 300 mm wide), connected to the upper cover through the hook at the top and connected to the funnel through the hook at the bottom. The trumpet connected to the insect collection bottle is placed on the lower part of the funnel and connected to the insect collection bottle.

[0097] b) Suspension of bait core: hang a bait core in the middle of the cross-plate bark beetle trap, 20 cm away from the top.

[0098] c) Field setting of traps: Three replicates were set for each experimental treatment. The traps were set at the edge of the spruce forest, and the distance between the traps was about 30m. Two 2m long bamboo poles with a diameter of about 5cm were inserted into the soil layer for about 50cm, and the distance between the two bamboo poles was 30cm. Then the trap was fixed between the two bamboo poles, and the insect collection bottle of the trap was about 50cm above the ground.

[0099] d) Data survey and trap emptying: In this experiment, each treatment was repeated three times, and the number of bark beetles trapped in each treatment was the average of the three traps. Depending on the weather conditions, the number of bark beetles in the insect collection bottle was observed every 2 to 3 days. After counting, the trapped bark beetles were placed in a plastic box and brought to the laboratory.

[0100] According to the above specific implementation steps, the forest biological activity test of Example 2 and Comparative Examples 8 to 11 of the light-hipped eight-toothed beetle was carried out from July 30 to August 30, 2022. The specific results are shown in Figure 2 In this experiment, each treatment was repeated three times, and the number of bark beetles trapped in each treatment was the average number of the three traps. Depending on the weather conditions, the number of bark beetles in the insect collection bottle was observed every 2 to 3 days.

[0101] from Figure 2 The analysis of the insect attractant effect shows that adding trans-2-hexenal to the conventional aggregation pheromone attractant ingredients of the light-hipped eight-toothed beetle shows insect attractant activity at 4 test doses of 20mg, 200mg, 400mg and 2g. There is no significant difference in the biological activity of trans-2-hexenal between 200mg and 400mg, both of which are much greater than the trapping amount at 20mg. The trapping amount of trans-2-hexenal is the highest at a dose of 200mg. Therefore, considering the attractant core's trapping effect in the forest, the duration of effectiveness, and the economic use, 200mg of trans-2-hexenal is selected as the best dose in practical applications.

[0102] Example 3

[0103] The components of the highly effective attractant for the light-hipped eight-toothed beetle produced in this embodiment are as follows:

[0104] 2-methyl-3-butene-2-ol, 4 g;

[0105] (±)-dienol, 40 mg;

[0106] (S)-cis-verbenol, 20 mg;

[0107] Trans-2-hexenal, 200 mg.

[0108] The sample purity, lure preparation method, lure storage, and implementation steps are the same as those in Example 1.

[0109] Comparative Example 12

[0110] The attractant is: 2-methyl-3-butene-2-ol 4g, (±)-beetdienoyl alcohol 40mg, (S)-cis-verbenol 20mg; the rest is the same as in Example 2, the results are shown in Figure 3 .

[0111] Comparative Example 13

[0112] No attractants, only bait.

[0113] Comparative Example 12 is to add (±)-beetdienolide, (S)-cis-verbenol and trans-2-hexenal directly to 2-methyl-3-butene-2-ol according to the set dosage. Comparative Example 13 is to use a trap with a blank bait core (consisting only of absorbent cotton, polyethylene plastic bottle and polyethylene plastic bag, without adding any other ingredients) as a blank control.

[0114] The attractant core of the light-hipped eight-toothed beetle made by using Example 3 and Comparative Examples 12 to 13 is used to attract the light-hipped eight-toothed beetle, and the specific steps are as follows:

[0115] a) Production of the trap: The trap is mainly a cross-plate type bark beetle trap purchased from Beijing Zhongjie Sifang Biotechnology Co., Ltd. The large plate and the small plate are crossed to form a cross shape (500 mm high and 300 mm wide), connected to the upper cover through the hook at the top and connected to the funnel through the hook at the bottom. The trumpet connected to the insect collection bottle is placed on the lower part of the funnel and connected to the insect collection bottle.

[0116] b) Suspension of bait core: hang a bait core in the middle of the cross-plate bark beetle trap, 20 cm away from the top.

[0117] c) Field setting of traps: Three replicates were set for each experimental treatment. The traps were set at the edge of the spruce forest, and the distance between the traps was about 30m. Two 2m long bamboo poles with a diameter of about 5cm were inserted into the soil layer for about 50cm, and the distance between the two bamboo poles was 30cm. Then the trap was fixed between the two bamboo poles, and the insect collection bottle of the trap was about 50cm above the ground.

[0118] d) Data survey and trap emptying: In this experiment, each treatment was repeated three times, and the number of bark beetles trapped in each treatment was the average of the three traps. Depending on the weather conditions, the number of bark beetles in the insect collection bottle was observed every 5 days. After counting, the trapped bark beetles were placed in a plastic box and brought to the laboratory.

[0119] According to the above specific implementation steps, the forest biological activity test of Example 3 and Comparative Examples 12-13 was carried out from July 23 to September 1, 2023, and the hanging light-hipped beetle aggregation pheromone attractant (Comparative Example 12) was used as a positive control, and the trap with a blank attractant core (Comparative Example 13) was used as a blank control. The specific results are shown in Figure 3 In this experiment, three replicates were set for each treatment, and the number of bark beetles trapped in each treatment was the average number of the three traps. Depending on the weather conditions, the number of bark beetles in the insect collection bottle was observed every 5 days.

[0120] from Figure 3 The analysis of the insect attractant effect shows that the conventional aggregation pheromone attractant of the light-hipped eight-toothed beetle (Comparative Example 12) has a good attracting effect during the entire monitoring and investigation period (July 23 to September 1), and can basically fully reflect the forest occurrence dynamics of the light-hipped eight-toothed beetle. The attracting effect of the high-efficiency attractant for the light-hipped eight-toothed beetle (Example 3) on the light-hipped eight-toothed beetle is better than that of the comparative example 12. In the early stage of the monitoring and investigation (July 23 to August 2), since the new generation of adults of the light-hipped eight-toothed beetle had just emerged and had not entered the peak period of flying, and was affected by the local rainfall climate, the amount of trapping was relatively small, but compared with the comparative example 12, trans-2-hexenal did not show a significant synergistic effect. As the local weather improves and the temperature rises, the light-hipped eight-toothed beetle enters the flying period, and trans-2-hexenal shows a very good synergistic effect on the attraction of the light-hipped eight-toothed beetle, and the monitoring sensitivity is significantly improved. The trapping amount is increased by 3 to 4 times compared with the comparative example 12, and the continuous synergistic time is up to about 50 days. This embodiment illustrates that the light-hipped eight-toothed beetle efficient attractant prepared by 2-methyl-3-butene-2-ol, (±)-beetle dienol, (S)-cis-verbenol and trans-2-hexenal can significantly improve the trapping amount of the light-hipped eight-toothed beetle, and trans-2-hexenal has a long-term synergistic effect on the conventional aggregation pheromone components of the light-hipped eight-toothed beetle, indicating that trans-2-hexenal can be used as an efficient attractant synergist for the trapping and killing of the light-hipped eight-toothed beetle, and more effectively monitors the population dynamics of the light-hipped eight-toothed beetle.

[0121] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. An attractant for the smooth-hipped eight-toothed beetle, It is characterized in that The composition comprises the following components in parts by weight: 4 parts of 2-methyl-3-butene-2-ol, 0.04 parts of (±)-dienol, ( S )-cis-verbenol 0.02 parts and trans-2-hexenal 0.2 parts.

2. Use of the attractant according to claim 1 in attracting the light-hipped eight-toothed beetle.

3. A bait for attracting the smooth-hipped eight-toothed beetle, It is characterized in that The invention comprises a slow-release carrier, absorbent cotton placed in the slow-release carrier and the attractant according to claim 1 adsorbed on the absorbent cotton.

4. The lure according to claim 3, It is characterized in that The sustained-release carrier is a polyethylene plastic bottle and a polyethylene plastic bag.

5. The lure according to claim 3, It is characterized in that In a single lure: The content of 2-methyl-3-butene-2-ol is 4 g; The content of (±)-dienol is 40 mg; ( S )-cis-verbenol content is 20 mg; The content of trans-2-hexenal was 0.2 g.

6. The lure according to claim 3, It is characterized in that The mass of the absorbent cotton in a single bait core is 5 g.

7. The lure according to claim 4, It is characterized in that The polyethylene plastic bottle has a capacity of 16 ml and a wall thickness of 0.6 mm; the polyethylene plastic bag has a thickness of 0.2 mm, a length of 150 mm and a width of 80 mm.

8. A method for preparing the lure according to any one of claims 3 to 7, It is characterized in that The following steps are involved: Place the absorbent cotton in a polyethylene plastic bottle, then drop the attractant on the absorbent cotton, and finally put it into a polyethylene plastic bag and seal it to obtain the attractant core.

9. Use of the bait described in any one of claims 3 to 7 in attracting the smooth-hipped eight-toothed beetle.

10. The use according to claim 9, It is characterized in that The application includes: using the bait core in combination with a cross-plate type bark beetle trap, and setting it at the edge of a spruce forest or in an open, sunny, ventilated area in the forest, with a spacing of 30 to 50 meters.

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