Uses of arenca palm starch

Abstract

The present invention provides the use of arenca palm powder in the preparation of a composition for preventing drunkenness, especially the use of arenca palm powder as the sole active ingredient in the preparation of a composition for preventing drunkenness. The present invention also provides a preparation method of the composition.

Description

Technical Field

[0001] The present invention belongs to the fields of medicine and health food, and particularly relates to a new use of arenga pinnata starch. Background Art

[0002] Alcohol is absorbed from the digestive tract and enters the brain about 10 minutes later. Ethanol can combine with lecithin and deposit in tissues for up to 0.5 - 1 month. Since the brain tissue is extremely rich in lecithin, alcohol and its metabolites can cause persistent direct and indirect damage to the nervous system.

[0003] To reduce the harm of alcohol to the body, especially the liver and central nervous system, taking substances with the effect of relieving hangover before drinking is a commonly used method. Although some hangover products (such as medicated diet recipes, beverages, drugs, etc.) have appeared in the prior art, they still cannot meet the demand.

[0004] Arenga westerhoutii Griffith is an arborescent plant of the genus Arenga in the palm family, distributed in Hainan, Guangxi and the western to southeastern parts of Yunnan in China. The starch processed from the pith of the arenga westerhoutii trunk through processes such as pulverization, slag removal, precipitation, filtration, and drying is called arenga pinnata starch. According to ancient books such as "Compendium of Materia Medica" and "Materia Medica of the Sea": "Arenga pinnata starch tastes sweet and flat, non-toxic. Making cakes and roasting them for food is delicious, making people not hungry, tonifying deficiency and weakness, relieving weakness of the waist and legs. Taking it for a long time can make the body light and avoid eating." However, so far, there has been no report on the effect of arenga pinnata starch on the metabolism of ethanol in the body. And the inventor has first discovered through research that arenga pinnata starch has the effect of relieving hangover. Summary of the Invention

[0005] Based on the inventor's research, the present invention provides a new use of arenga pinnata starch.

[0006] For this reason, the present invention adopts the following technical solutions:

[0007] Use of arenga pinnata starch in the preparation of a composition for preventing drunkenness.

[0008] Preferably, the composition may further include other substances that can shorten the metabolism time of ethanol in the body and reduce the harmful effect of ethanol.

[0009] More preferably, the substances are selected from one or more of vitamin C, B vitamins, honey, mung bean, kudzu root, sweet potato, fresh lotus root, lotus root starch, chrysanthemum, and ophiopogon japonicus.

[0010] As a preferred embodiment, the present invention provides the following technical solution:

[0011] Use of arenga pinnata starch as the only active ingredient in the preparation of a composition for preventing drunkenness.

[0012] Preferably, the anti-hangover composition may further include acceptable excipients.

[0013] Preferably, the mass of the excipient accounts for 0-20% of the total mass of the anti-hangover composition.

[0014] Preferably, the excipient is selected from one or more of water, vegetable oil, microcrystalline cellulose, starch, dextrin, lactose, powdered sugar, and honey.

[0015] Preferably, the anti-hangover composition is in the form of powder, capsules, granules, tablets, or oral liquid.

[0016] Preferably, the anti-hangover composition is prepared by the following method:

[0017] Take areca palm powder, with or without adding other substances that can shorten the in-vivo metabolism time of ethanol and reduce the damage of ethanol, add the excipient, and prepare it into powder, capsules, granules, tablets, or oral liquid according to the general methods in the art.

[0018] The administration object of the anti-hangover composition of the present invention is mammals, and more preferably humans.

[0019] When the administration object is human, the anti-hangover composition is administered to those in need before drinking, 1-2 grams of areca palm powder per time.

[0020] The areca palm powder must meet relevant standards, such as the local standard of Guangxi Zhuang Autonomous Region, "DBS 45 / 044-2017 Local Food Safety Standard Instant Areca Palm Powder". Detailed implementation manners

[0021] The following refers to specific examples to illustrate the present invention. Those skilled in the art can understand that these examples are only used to illustrate the present invention and do not limit the scope of the present invention in any way.

[0022] The experimental methods in the following examples are all conventional methods unless otherwise specified. The raw materials, reagent materials, etc. used in the following examples are all commercially available products unless otherwise specified.

[0023] Test Example 1 The anti-hangover effect of areca palm powder on zebrafish

[0024] 1. Experimental animals

[0025] Wild-type AB strain zebrafish, reproduced by natural paired mating. The age is 5 dpf (5 days after fertilization), with a total of 360 tails, and each experimental group has 30 tails (used to determine the maximum detection concentration of areca palm powder and evaluate the anti-hangover effect).

[0026] The above zebrafish were all raised in fish-raising water at 28°C (water quality: 200 mg of instant sea salt was added to every 1 L of reverse osmosis water, the conductivity was 480 - 510 μS / cm; pH was 6.9 - 7.2; hardness was 53.7 - 71.6 mg / L CaCO3). The experimental animal use license number was: SYXK(Zhe)2012 - 0171. The feeding management complied with the requirements of international AAALAC accreditation.

[0027] 2. Information of Test Articles

[0028] Arrowroot powder, white powder, provided by the Engineering Research Center of Natural Medicines of the Ministry of Education, Beijing Normal University, compliant with the standard "DBS 45 / 044 - 2017 Local Food Safety Standard for Ready-to-Eat Arrowroot Powder", stored in a normal-temperature cool cabinet. Prepare a stock solution of 20 mg / mL with fish-raising water before use and use it immediately after preparation.

[0029] Absolute ethanol, purchased from Sinopharm Chemical Reagent Co., Ltd., batch number 20181210, stored at normal temperature in dry and dark conditions, and used directly.

[0030] Ru21 (Antep Natural Plant Extract Composite Tablets), yellow solid, produced by Spirit Sciences USA, Inc (USA Mental Sciences Company), batch number CA90274, stored in a cool cabinet in the dark. Prepare a stock solution of 100 mg / mL with fish-raising water before use.

[0031] 3. Instruments and Reagents

[0032] Dissecting microscope (SZX7, OLYMPUS, Japan); camera connected to the microscope (VertA1); precision electronic balance (CP214, OHAUS, America); motion / behavior analysis recorder (V3.11, ViewPoint Life Sciences, France); 6-well plates and 96-well plates (Fisher Scientific, China); absolute ethanol (batch number 20170918, Sinopharm Group).

[0033] 4. Concentration Groups

[0034] 4.1 Determine the maximum test concentration (MTC) of arrowroot powder

[0035]

[0036] 4.2 Evaluate the anti-hangover effect of arrowroot powder on zebrafish

[0037]

[0038] Based on the fact that zebrafish showed no obvious abnormalities under the concentration condition of 1000 μg / mL of arenca palm powder, the highest detection concentration of arenca palm powder for zebrafish was determined to be 1000 μg / mL.

[0039] 5. Model preparation

[0040] Treat 6-dpf wild-type AB strain zebrafish with 1% absolute ethanol to establish an excited-phase drunkenness model of zebrafish.

[0041] 6. Experimental methods

[0042] 6.1 Determine the maximum test concentration (MTC) of arenca palm powder

[0043] Randomly select 150 5-day post-fertilization (5-dpf) wild-type AB strain zebrafish into six-well plates, with 30 zebrafish in each well (experimental group). Treat them with arenca palm powder at concentrations of 125, 250, 500, and 1000 μg / mL in water solution respectively. At the same time, set up a normal control group (zebrafish treated with fish-raising water) and a model control group. The volume of each well (experimental group) is 3 mL. After treating with arenca palm powder for 24 h, observe and record the toxic phenotypes and death conditions of zebrafish to determine the maximum test concentration of arenca palm powder for zebrafish in the excited-phase drunkenness model induced by absolute ethanol.

[0044] 6.2 Evaluate the anti-hangover effect of arenca palm powder on zebrafish

[0045] Randomly select 180 5-day post-fertilization (5-dpf) wild-type AB strain zebrafish into six-well plates, with 30 zebrafish in each well (experimental group). Treat them with arenca palm powder at concentrations of 222.2 and 666.7 μg / mL in water solution respectively, and the positive control drug "Ru21" at a concentration of 100 μg / mL. At the same time, set up a normal control group (zebrafish treated with fish-raising water) and a model control group. The volume of each well (experimental group) is 3 mL. After treating with arenca palm powder and "Ru21" for 24 h respectively, randomly select 10 zebrafish from each group and place them in 96-well plates, one in each well, and the volume of each well is 200 μL. Except for the normal control group, the remaining experimental groups are treated with absolute ethanol in water solution to induce zebrafish to establish an excited-phase drunkenness model. Use a motion / behavior analyzer to record the movement of zebrafish within 1 h after giving absolute ethanol. Analyze and statistically analyze the total movement distance (D) of zebrafish. Evaluate the anti-hangover effect of arenca palm powder on zebrafish in the excited-phase drunkenness model induced by absolute ethanol based on the statistical analysis results of the total movement distance. The statistical processing results are expressed as mean ± SE. The calculation formula for the anti-hangover effect is as follows:

[0046]

[0047] Perform statistical analysis using analysis of variance and Dunnett's T-test. p < 0.05 indicates a significant difference.

[0048] 7. Experimental Results

[0049] 7.1 MTC

[0050] At a concentration of 1000 μg / mL of areca nut starch, no precipitation was observed, and no obvious abnormalities were found in zebrafish. Therefore, the highest detection concentration of areca nut starch for zebrafish was determined to be 1000 μg / mL. The results are shown in Table 1 for details.

[0051] Table 1 "Concentration - Lethality" Results of Areca Nut Starch Treatment (n = 30)

[0052]

[0053] 7.2 Antidotal Effect of Areca Nut Starch on Zebrafish

[0054] The results are shown in Table 2 for details.

[0055] Table 2 Antidotal Effect Results of Areca Nut Starch Treatment on Zebrafish (n = 10)

[0056]

[0057]

[0058] *: Compared with the model control group, p < 0.05.

[0059] The data in Table 2 show that:

[0060] (1) The total movement distance of zebrafish in the model control group (9720 mm) compared with that in the normal control group (5452 mm), p < 0.001, indicating that the model was successfully established. The total movement distance of zebrafish in the positive control drug "Ru21" 100 μg / mL concentration group was 6962 mm. Compared with the model control group, p < 0.05, and the antidotal effect was 65%, indicating that "Ru21" has an antidotal effect on zebrafish in the excited - stage drunkenness model induced by absolute ethanol. There was no antidotal effect in the wheat flour group compared with the model control group.

[0061] (2) The total movement distances of zebrafish in the areca nut starch 222 and 667 μg / mL concentration groups were 7558 mm and 6918 mm respectively. Compared with the model control group, p < 0.05 in the 222 and 667 μg / mL concentration groups, and the antidotal effects were 51% and 66% respectively; suggesting that areca nut starch has an obvious antidotal effect on zebrafish in the excited - stage drunkenness model induced by absolute ethanol under the concentration conditions of 222 and 667 μg / mL.

[0062] Test Example 2

[0063] 1. Experimental Animals

[0064] Wild-type AB strain zebrafish were bred by natural pair mating. They were 5 dpf (5 days post-fertilization) old, with a total of 360 fish, and 30 fish in each experimental group.

[0065] The above zebrafish were all raised in fish-raising water at 28 °C (water quality: 200 mg of instant sea salt was added to every 1 L of reverse osmosis water, the conductivity was 480 - 510 μS / cm; the pH was 6.9 - 7.2; the hardness was 53.7 - 71.6 mg / L CaCO3). The experimental animal use license number was: SYXK(Zhe)2012 - 0171. The feeding management met the requirements of international AAALAC certification.

[0066] 2. Information of test articles

[0067] Areca powder, white powder, provided by the Engineering Research Center of Natural Medicines of the Ministry of Education, Beijing Normal University, meeting the standard of "DBS 45 / 044 - 2017 Local Food Safety Standard Ready-to-eat Areca Powder", and stored in a normal temperature cool cabinet. When in use, it was prepared into a stock solution of 20 mg / mL with fish-raising water for standby, and prepared and used immediately.

[0068] Absolute ethanol, purchased from Sinopharm Chemical Reagent Co., Ltd., batch number 20181210, stored at normal temperature in dry and dark place, and used directly.

[0069] Ru21 (Antipu Natural Plant Extract Composite Tablets), yellow solid, produced by Spirit Sciences USA, Inc (USA), batch number CA90274, stored in a cool cabinet in the dark, and prepared into a stock solution of 100 mg / mL with fish-raising water for standby when in use.

[0070] 3. Instruments and reagents

[0071] Dissecting microscope (SZX7, OLYMPUS, Japan); camera connected to the microscope (VertA1); precision electronic balance (CP214, OHAUS, America); motion / behavior analysis recorder (V3.11, ViewPoint Life Sciences, France); 6-well plates and 96-well plates (Fisher Scientific, China); absolute ethanol (batch number 20170918, Sinopharm Group).

[0072] 4. Experimental grouping

[0073]

[0074] 5. Model establishment

[0075] Wild-type AB strain zebrafish at 6 dpf were treated with 1% absolute ethanol to establish a zebrafish excitatory stage drunkenness model.

[0076] 6.2 Evaluation of the hangover effect of arenca palm starch on zebrafish

[0077] Randomly select 120 wild-type AB strain zebrafish at 5 days post-fertilization (5 dpf) and place them in a six-well plate, with 30 zebrafish in each well (experimental group). Treat the zebrafish with 444.4 μg / mL concentration of arenca palm starch and 100 μg / mL concentration of the positive control drug "Ru21" by dissolving them in water. At the same time, set up a normal control group (treating zebrafish with fish-raising water) and a model control group. The volume of each well (experimental group) is 3 mL. After treating with arenca palm starch and "RU21" for 24 h respectively, randomly select 10 zebrafish from each group and place them in a 96-well plate, with 1 zebrafish in each well and the volume of each well being 200 μL. Except for the normal control group, the other experimental groups are treated with absolute ethanol dissolved in water to induce an excited-stage drunkenness model in zebrafish. Use a motion / behavior analyzer to record the movement of zebrafish within 1 h after giving absolute ethanol. Analyze and statistically analyze the total movement distance (D) of zebrafish. Evaluate the hangover effect of arenca palm starch on zebrafish in the excited-stage drunkenness model induced by absolute ethanol based on the statistical analysis results of the total movement distance. The statistical processing results are expressed as mean±SE. The calculation formula for the hangover effect is as follows:

[0078]

[0079] Perform statistical analysis using analysis of variance and Dunnett's T-test. p<0.05 indicates a significant difference.

[0080] 7. Experimental results

[0081] 7.2 Hangover effect of arenca palm starch on zebrafish, and the results are shown in Table 3..

[0082] Table 3 Results of the hangover effect of zebrafish after treatment with arenca palm starch (n = 10)

[0083]

[0084]

[0085] *: Compared with the model control group, p<0.05.

[0086] The data in Table 3 show that: The total movement distance of zebrafish in the model control group (9611 mm) compared with that in the normal control group (5534 mm), p < 0.001, indicating that the model was successfully established. The total movement distance of zebrafish in the positive control drug "Ru21" 100 μg / mL concentration group was 7441 mm, compared with the model control group, p < 0.05, and the anti-alcoholism effect was 53%, indicating that "Ru21" has an anti-alcoholism effect on zebrafish in the excited stage drunkenness model induced by absolute ethanol. The total movement distance of zebrafish in the areca nut powder 444.4 μg / mL concentration group was 7333 mm respectively. Compared with the model control group, p < 0.05, and the anti-alcoholism effect was 56%, suggesting that it has an obvious anti-alcoholism effect on zebrafish in the excited stage drunkenness model induced by absolute ethanol.

[0087] In summary, the present invention provides a new use of areca nut powder in the preparation of a composition for preventing drunkenness, opening up a new use for areca nut powder. Areca nut powder itself is edible, so its safety is good and it will not cause harm to users.

Claims

1. Use of areca nut starch as the only active ingredient in the preparation of a composition for preventing drunkenness.

2. The application according to claim 1, wherein The composition for preventing drunkenness further comprises acceptable excipients.

3. The application according to claim 2, wherein The mass of the excipients accounts for 0-20% of the total mass of the composition for preventing drunkenness.

4. The application according to claim 2 or 3, characterized in that, The excipients are selected from one or more of water, vegetable oil, microcrystalline cellulose, starch, dextrin, lactose, powdered sugar and honey.

5. The application according to any one of claims 1 to 3, characterized in that, The composition for preventing drunkenness is a powder, capsule, granule, tablet or oral liquid.

6. The application according to claim 4, characterized in that The composition for preventing drunkenness is a powder, capsule, granule, tablet or oral liquid.

7. The application according to claim 5, wherein The composition for preventing drunkenness is prepared by the following method: Take areca nut starch, add the excipients, and prepare into a powder, capsule, granule, tablet or oral liquid according to the methods common in the art.

8. The application according to claim 6, characterized in that, The composition for preventing drunkenness is prepared by the following method: Take areca nut starch, add the excipients, and prepare into a powder, capsule, granule, tablet or oral liquid according to the methods common in the art.

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