Method, device, medium and equipment for judging insufficient dose of hemolytic agent

Abstract

This invention discloses a method, apparatus, medium, and device for determining insufficient hemolytic agent dosage. First, a set of pulse signals from a blood cell sample under a preset scattering angle is acquired. Then, the signal intensity of each pulse signal in the set is identified, and the particle distribution is statistically analyzed. Next, the rate of change of particle count for each type of signal intensity in the particle distribution is calculated to obtain the rate of change. Then, the zero-crossing points of the rate of change are searched for, and the maximum particle count and the minimum particle count associated with red blood cells are determined based on these points. Finally, the signal interference value is calculated based on the minimum and maximum particle counts. If the signal interference value is greater than an interference threshold, the current hemolytic agent dosage is determined to be insufficient. This invention can promptly remind testing personnel to check the remaining reagent level when significant interference is detected by monitoring the degree of red blood cell interference in the pulse signal.

Description

Technical Field

[0001] This invention relates to the field of hemolytic agents, and in particular to a method, apparatus, medium, and device for determining insufficient hemolytic agent dosage. Background Technology

[0002] A blood cell analyzer counts various cells in the blood. When a 5LDS hemolysin is mixed with a fresh blood sample, red blood cells are dissolved, while white blood cells are stained. The stained white blood cells and red blood cell fragments, encased in sheath fluid, cause the test cells to arrange themselves in a single row and flow uniformly into the flow chamber. Under the illumination of a laser beam, scattered light is generated at three different angles. The magnitude of the scattered light generated by the cells irradiated by the laser beam is related to the cell size, the refractive index of the cell membrane, and the complexity of the internal organs. The scattered light signal is ultimately converted into an electrical pulse signal. Based on the collected electrical pulse data, a scatter plot distribution of white blood cells in a three-dimensional signal can be obtained. Finally, the classification result of white blood cells is obtained based on the Differentiation Scattergram (DIFF). The detection principle is as follows: Figure 1 As shown.

[0003] As is well known, red blood cells are approximately 6-9 μm in diameter, while lymphocytes are 5-20 μm in diameter. If the required dose of 5LDS hemolysin in the reaction system of the sample to be tested is insufficient, the red blood cells will not be fully dissolved. Furthermore, since the volumes of red blood cells and lymphocytes are relatively similar, this will ultimately affect the accuracy of white blood cell classification. Therefore, there is an urgent need for a method to determine whether the white blood cell classification process is interfered with by red blood cell fragments, so as to remind the testing personnel to retest or check the remaining 5LDS reagent. Summary of the Invention

[0004] Therefore, it is necessary to provide methods, devices, media, and equipment for determining insufficient hemolytic agent dosage in order to solve the problem of inaccurate white blood cell detection caused by insufficient hemolytic agent dosage.

[0005] A method for determining insufficient dosage of hemolytic agent, the method comprising:

[0006] The system acquires a set of pulse signals from a blood cell sample under a preset scattering angle, identifies the signal intensity of each pulse signal in the set, and statistically analyzes the particle distribution of the signal intensity. The particle distribution is used to indicate the number of blood cell particles with different signal intensities. The blood cell sample under test is a blood cell sample obtained after treatment with the current hemolytic agent.

[0007] Calculate the rate of change of the number of particles for each type of signal intensity in the particle distribution to obtain the rate of change.

[0008] Search for the zero-crossing point of the rate of change and determine the maximum number of particles in the particle distribution based on the searched zero-crossing point.

[0009] Search for the rising zero-crossing point in the rate of change case, and determine the minimum number of particles associated with red blood cells in the particle distribution case based on the searched rising zero-crossing point;

[0010] The signal interference value is calculated based on the minimum and maximum particle number values. If the signal interference value is greater than a preset interference threshold, the current dose of hemolytic agent is determined to be insufficient. The signal interference value is used to indicate the degree of interference of the pulse signal by red blood cells.

[0011] In one embodiment, the formula for calculating the particle number change rate is:

[0012]

[0013] In the above formula, The rate of change of the particle number indicating the intensity of the i-th type of signal. The number of blood cell particles indicating the intensity of the (i+1)th type of signal. N indicates the number of blood cell particles representing the intensity of signal i, and N indicates the total number of signal classes.

[0014] In one embodiment, the step of searching for a zero-crossing point in the rate of change and determining a particle number maxima in the particle distribution based on the found zero-crossing point includes:

[0015] Search for all zero-crossing points in the rate of change scenario, and obtain the number of particles corresponding to each zero-crossing point in the particle distribution scenario as candidate particle counts;

[0016] The maximum value among all candidate particle counts is taken as the maximum particle count.

[0017] In one embodiment, the step of searching for a rising zero-crossing point in the rate of change and determining a minimum number of particles associated with red blood cells in the particle distribution based on the searched rising zero-crossing point includes:

[0018] Search for the first zero-crossing point in the rate of change scenario, and take the number of particles corresponding to the first zero-crossing point in the particle distribution scenario as the minimum value of the particle number.

[0019] In one embodiment, calculating the signal interference value based on the minimum and maximum particle number includes:

[0020] Calculate the ratio of the minimum particle number to the maximum particle number, and use the ratio as the interference value of the signal.

[0021] In one embodiment, after the particle distribution of the statistical signal intensity, the method further includes:

[0022] The particle distribution is filtered; the filtering formula is as follows:

[0023]

[0024] In the above formula, This shows the particle distribution after filtering. This represents the particle distribution before filtering, where i indicates the intensity of the i-th type of signal, and N indicates the total number of signal types. Indicator mean, Indicative standard deviation.

[0025] A device for determining insufficient dosage of hemolytic agent, the device comprising:

[0026] The particle distribution determination module is used to acquire the pulse signal set of the blood cell sample to be tested at a preset scattering angle, identify the signal intensity of each pulse signal in the pulse signal set, and count the particle distribution of the signal intensity; wherein, the particle distribution is used to indicate the number of blood cell particles with different signal intensities, and the blood cell sample to be tested is a blood cell sample obtained after treatment with the current hemolytic agent.

[0027] The extreme point determination module is used to calculate the rate of change of the number of particles for each type of signal intensity in the particle distribution to obtain the rate of change situation; search for the falling zero-crossing point in the rate of change situation, and determine the maximum number of particles in the particle distribution based on the searched falling zero-crossing point; search for the rising zero-crossing point in the rate of change situation, and determine the minimum number of particles related to red blood cells in the particle distribution based on the searched rising zero-crossing point.

[0028] The judgment module is used to calculate the signal interference value based on the minimum and maximum particle counts. If the signal interference value is greater than a preset interference threshold, it is determined that the current dose of hemolytic agent is insufficient. The signal interference value is used to indicate the degree of interference of the pulse signal by red blood cells.

[0029] A computer-readable storage medium storing a computer program, which, when executed by a processor, causes the processor to perform the steps of the above-described method for determining insufficient hemolytic agent dosage.

[0030] A device for determining insufficient hemolytic agent dosage includes a memory and a processor. The memory stores a computer program, which, when executed by the processor, causes the processor to perform the steps of the aforementioned method for determining insufficient hemolytic agent dosage.

[0031] This invention provides a method, apparatus, medium, and device for determining insufficient hemolytic agent dosage. First, a set of pulse signals from a blood cell sample under a preset scattering angle is acquired. The signal intensity of each pulse signal in the set is identified, and the particle distribution of the signal intensity is statistically analyzed. Further, the rate of change of the number of particles for each type of signal intensity in the particle distribution is calculated to obtain the rate of change. Further, a zero-crossing point in the rate of change is searched, and a maximum particle number is determined based on the found zero-crossing point. Further, a zero-crossing point in the rate of change is searched, and a minimum particle number related to red blood cells is determined based on the found zero-crossing point. Finally, a signal interference value is calculated based on the minimum and maximum particle numbers. If the signal interference value is greater than a preset interference threshold, the current hemolytic agent dosage is determined to be insufficient. This signal interference value indicates the degree of interference of red blood cells with the pulse signal. As can be seen, the present invention can determine the degree of interference of pulse signals by red blood cells based on the monitored pulse signal set. When there is significant interference, it can promptly remind the testing personnel to retest or check the remaining amount of reagents, thereby improving the accuracy of white blood cell classification parameter detection. Attached Figure Description

[0032] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0033] in:

[0034] Figure 1 This is a schematic diagram illustrating the principle of white blood cell monitoring.

[0035] Figure 2 A flowchart illustrating the method for determining insufficient hemolytic agent dosage;

[0036] Figure 3 A schematic diagram illustrating the generation of scattered light at three different angles;

[0037] Figure 4 This is a schematic diagram of the DIFF of leukocytes and the particle distribution under conditions of sufficient hemolytic agent.

[0038] Figure 5 for Figure 4A schematic diagram of extreme points in particle distribution;

[0039] Figure 6 This is a schematic diagram of the DIFF of leukocytes and the particle distribution under conditions of insufficient hemolytic agent.

[0040] Figure 7 A schematic diagram of a device for determining insufficient hemolytic agent dosage;

[0041] Figure 8 This is a block diagram of a device for determining insufficient hemolytic agent dosage. Detailed Implementation

[0042] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0043] The terms "first," "second," etc., in the specification, claims, and accompanying drawings of this application are used to distinguish different objects, not to describe a specific order. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion. For example, a process, method, system, product, or apparatus that includes a series of steps or units is not limited to the listed steps or units, but may optionally include steps or units not listed, or may optionally include other steps or units inherent to these processes, methods, products, or apparatuses.

[0044] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0045] like Figure 2 As shown, Figure 2 This is a flowchart illustrating a method for determining insufficient hemolytic agent dosage in one embodiment. The steps provided by the method for determining insufficient hemolytic agent dosage in this embodiment include:

[0046] S201, acquire the pulse signal set of the blood cell sample to be tested at a preset scattering angle, identify the signal intensity of each pulse signal in the pulse signal set, and count the particle distribution of the signal intensity.

[0047] The particle distribution is used to indicate the number of blood cell particles with different signal intensities, and the blood cell sample to be tested is a blood cell sample obtained after treatment with the current hemolytic agent.

[0048] For example, in one scenario, the blood cell sample to be tested is first treated with the current 5LDS hemolysin. After treatment, the red blood cells are dissolved into red blood cell fragments, while the white blood cells are stained. These red blood cell fragments and different types of white blood cells are clearly distinguishable under the combination of three-dimensional scattered light signals. Specifically, encapsulated in sheath fluid, the cells are arranged in a single row and flow into the flow chamber at a uniform speed. Under the irradiation of the laser beam, as referenced... Figure 3 This generates scattered light at three different angles: low-angle, medium-angle, and high-angle scattered light. Low-angle scattered light is scattered light from the forward low-angle region, medium-angle scattered light is scattered light from the forward medium-angle region, and high-angle scattered light is scattered light from the side high-angle region. Low-angle scattered light reflects cell size, medium-angle scattered light reflects the fine internal structure and granular material of the cell, and high-angle forward scattered light also reflects the fine internal structure and granular material of the cell. The aperture in the receiving section is used to determine the presence of scattered light. The first receiver receives the medium-angle scattered light emitted from the flow chamber and converts it into a medium-angle pulse signal, forming the pulse signal set corresponding to the medium angle. The second receiver receives the high-angle scattered light emitted from the flow chamber and converts it into a high-angle pulse signal, forming the pulse signal set corresponding to the high angle. The third receiver receives the low-angle scattered light emitted from the flow chamber and converts it into a low-angle pulse signal, forming the pulse signal set corresponding to the low angle.

[0049] As can be seen, in the above scenario, if the dose of the required 5LDS hemolytic agent in the reaction system of the sample to be tested is insufficient, the red blood cells will not be fully dissolved. Furthermore, since the volume of red blood cells and lymphocytes is relatively similar, it will ultimately affect the accuracy of white blood cell classification.

[0050] Furthermore, existing pulse recognition algorithms are used to identify the signal strength of each pulse signal in the pulse signal set, such as threshold detection algorithms or energy threshold algorithms. By summing the signal strengths of all pulse signals, we can obtain... , indicating the first in the sample The signal intensity of the pulse signal of each white blood cell, among which This indicates the total number of pulse signals.

[0051] Furthermore, based on these signal intensities, the DIFF of white blood cells in three-dimensional signals can be obtained, for example, such as Figure 4 As shown in (a), the Y-axis (LS) represents the signal intensity of low-angle scattered light, and the X-axis (MS) represents the signal intensity of mid-angle scattered light. Of course, this scatter plot can also be constructed based on scattered light from other angles.

[0052] Furthermore, the particle distribution of the statistical signal intensity is analyzed, and this particle distribution is used to indicate the number of blood cell particles with different signal intensities. Instruction No. The number of particles of each signal strength class, where N is the total number of signal strength classes. For example, corresponding to Figure (4), ... Figure 4 The scatter plot in (a) can be mapped along the LS direction to obtain... Figure 4 (b) shows the particle distribution. It is clear that... Figure 4 The clusters in (a) will Figure 4 (b) shows the formation of corresponding pulse peaks, with both the clusters and pulse peaks representing a type of white blood cell. Based on this particle distribution, the particles can be preliminarily classified.

[0053] In one specific embodiment, the particle distribution is further filtered; wherein the filtering formula is:

[0054]

[0055] In the above formula, This shows the particle distribution after filtering. This represents the particle distribution before filtering, where i indicates the intensity of the i-th type of signal, and N indicates the total number of signal types. Indicator mean, Indicative standard deviation.

[0056] After the above filtering process, noise in the particle distribution can be effectively removed, making the overall distribution smoother.

[0057] S202, calculate the rate of change of the number of particles for each type of signal intensity in the particle distribution to obtain the rate of change.

[0058] Based on this rate of change, we can understand the trend of particle number changes at each signal intensity in the particle distribution.

[0059] In one specific embodiment, the formula for calculating the particle number change rate of the i-th type of signal intensity is:

[0060]

[0061] In the above formula, The rate of change of the particle number indicating the intensity of the i-th type of signal. The number of blood cell particles indicating the intensity of the (i+1)th type of signal. N indicates the number of blood cell particles representing the intensity of signal i, and N indicates the total number of signal classes.

[0062] S203, search for the zero-crossing point of the rate of change and determine the maximum number of particles in the particle distribution based on the searched zero-crossing point.

[0063] In the case of rate of change, a local range to the left of the zero-crossing point indicates that the number of particles with increasing signal strength in the corresponding particle distribution is continuously increasing; a local range to the right of the zero-crossing point indicates that the number of particles with increasing signal strength in the corresponding particle distribution is continuously decreasing. Based on these conditions, the zero-crossing point can be found in the case of rate of change. Optionally, the zero-crossing point can be defined as the rate of change of the number of particles for a signal strength of type i. In general, a point is considered to be a zero-crossing point of descent if the following conditions are met:

[0064]

[0065] Then, based on these zero-crossing points of descent found, the local maximum number of particles can be determined in the particle distribution, and further, the maximum value of the particle number can be found.

[0066] In one specific embodiment, the maximum particle number is determined as follows: search for all zero-crossing points in the rate of change case, and obtain the particle number corresponding to each zero-crossing point in the particle distribution case as a candidate particle number; the maximum value among all candidate particle numbers is taken as the maximum particle number.

[0067] For example, in Figure 5 In the process, the particle counts corresponding to A, B, and C are determined as candidate particle counts. Since A has the largest number of candidate particles, it is taken as the maximum particle count. This maximum particle count primarily serves as a reference value in subsequent calculations.

[0068] S204, search for the rising zero-crossing point in the rate of change case, and determine the minimum number of particles related to red blood cells in the particle distribution case based on the searched rising zero-crossing point.

[0069] In the case of rate of change, a local range to the left of the zero-crossing point indicates that the number of particles with signal strength in the corresponding particle distribution is continuously decreasing; a local range to the right of the zero-crossing point indicates that the number of particles with signal strength in the corresponding particle distribution is continuously increasing. Based on these conditions, the zero-crossing point can be found in the case of rate of change. Optionally, the zero-crossing point can be defined as the rate of change of the number of particles for a signal strength of type i. In general, if the following conditions are met, it is determined to be an upward zero-crossing point:

[0070]

[0071] Then, based on these zero-crossing points found, the local minimum number of particles can be determined in the particle distribution, and the minimum particle number can be found further.

[0072] In one specific embodiment, the minimum particle number is determined by searching for the first zero-crossing point in the rate of change case, and taking the particle number corresponding to the first zero-crossing point in the particle distribution case as the minimum particle number.

[0073] This is because red blood cells are approximately 6-9 μm in diameter, while lymphocytes are 5-20 μm in diameter; their volumes are relatively similar. Figure 6 As shown in (a), if the red blood cells are not fully lysed due to insufficient dosage of the hemolytic agent, then... Figure 6 (a) shows numerous interfering points within the white box, and these interfering points will appear in the mapped area. Figure 6 (b) The trough corresponding to the box area causes the local minimum particle number to increase significantly. Therefore, the local minimum particle number at this location is the minimum particle number related to red blood cells. Thus, in this specific embodiment, "the first rising zero-crossing point in the rate of change is searched, and the particle number corresponding to the first rising zero-crossing point is taken as the minimum particle number in the particle distribution."

[0074] Correspondingly, when there is sufficient hemolytic agent, Figure 5 In the process, it was determined that the signal intensity corresponding to D is the minimum particle number.

[0075] S205, calculate the signal interference value based on the minimum and maximum particle number. If the signal interference value is greater than the preset interference threshold, determine that the current dose of hemolytic agent is insufficient.

[0076] The interference value of this signal is used to indicate the degree to which the pulse signal is interfered with by red blood cells.

[0077] In one specific embodiment, the signal interference value is calculated as follows: the ratio of the minimum particle number to the maximum particle number is calculated, and this ratio is used as the signal interference value, expressed as:

[0078]

[0079] In the above formula, The value of interference affecting the indicator signal; Indicator particle number minimum; This indicates the maximum particle number. Of course, the interference value of this signal can also be changed to other forms.

[0080] See Figure 4 and Figure 5 As can be seen from the embodiments, If the value is relatively small, and less than the preset interference threshold, then the current dose of hemolytic agent is considered sufficient. (See also...) Figure 6 As can be seen from the embodiments, If the value is too large, and exceeds the preset interference threshold, then the current dose of hemolytic agent is determined to be insufficient.

[0081] The above method first acquires a set of pulse signals from the blood cell sample under a preset scattering angle, identifies the signal intensity of each pulse signal in the set, and statistically analyzes the particle distribution of the signal intensity. Further, it calculates the rate of change of the number of particles for each type of signal intensity in the particle distribution to obtain the rate of change. Next, it searches for a zero-crossing point in the rate of change and determines the maximum particle number in the particle distribution based on this zero-crossing point. Then, it searches for a zero-crossing point in the rate of change and determines the minimum particle number associated with red blood cells based on this zero-crossing point. Finally, it calculates the signal interference value based on the minimum and maximum particle numbers. If the signal interference value is greater than a preset interference threshold, the current dose of hemolytic agent is deemed insufficient. This signal interference value indicates the degree of interference of the pulse signal by red blood cells. Therefore, this invention can determine the degree of interference of the pulse signal by red blood cells based on the monitored pulse signal set. When significant interference occurs, it promptly reminds the testing personnel to retest or check the remaining reagent level, thereby improving the accuracy of white blood cell classification parameter detection.

[0082] In one embodiment, such as Figure 7 As shown, a device for determining insufficient hemolytic agent dosage is proposed, the device comprising:

[0083] The particle distribution determination module 701 is used to acquire the pulse signal set of the blood cell sample to be tested at a preset scattering angle, identify the signal intensity of each pulse signal in the pulse signal set, and count the particle distribution of the signal intensity; wherein, the particle distribution is used to indicate the number of blood cell particles with different signal intensities, and the blood cell sample to be tested is a blood cell sample obtained after treatment with the current hemolysing agent.

[0084] The extreme point determination module 702 is used to calculate the rate of change of the number of particles for each type of signal intensity in the particle distribution to obtain the rate of change situation; search for the decreasing zero-crossing point in the rate of change situation, and determine the maximum number of particles in the particle distribution based on the searched decreasing zero-crossing point; search for the increasing zero-crossing point in the rate of change situation, and determine the minimum number of particles related to red blood cells in the particle distribution based on the searched increasing zero-crossing point.

[0085] The judgment module 703 is used to calculate the signal interference value based on the minimum and maximum particle number values. If the signal interference value is greater than the preset interference threshold, it is determined that the current dose of hemolytic agent is insufficient. The signal interference value is used to indicate the degree of interference of the pulse signal by red blood cells.

[0086] In one embodiment, the formula for calculating the rate of change of particle number is:

[0087]

[0088] In the above formula, The rate of change of the particle number indicating the intensity of the i-th type of signal. The number of blood cell particles indicating the intensity of the (i+1)th type of signal. N indicates the number of blood cell particles representing the intensity of signal i, and N indicates the total number of signal classes.

[0089] In one embodiment, the extreme point determination module 702 is specifically used to: search for all zero-crossing points in the rate of change case, and obtain the number of particles corresponding to each zero-crossing point in the particle distribution case as a candidate particle number; and take the maximum value among all candidate particle numbers as the maximum particle number.

[0090] In one embodiment, the extreme point determination module 702 is specifically used to: search for the first rising zero-crossing point in the rate of change case, and take the number of particles corresponding to the first rising zero-crossing point as the minimum number of particles in the particle distribution case.

[0091] In one embodiment, the judgment module 703 is specifically used to: calculate the ratio of the minimum particle number to the maximum particle number, and use the ratio as the signal interference value.

[0092] In one embodiment, after statistically analyzing the particle distribution of the signal intensity, the method further includes:

[0093] The particle distribution is filtered; the filtering formula is as follows:

[0094]

[0095] In the above formula, This shows the particle distribution after filtering. This represents the particle distribution before filtering, where i indicates the intensity of the i-th type of signal, and N indicates the total number of signal types. Indicator mean, Indicative standard deviation.

[0096] Figure 8 An internal structural diagram of a device for determining insufficient hemolytic agent dosage is shown in one embodiment. Figure 8 As shown, the device for determining insufficient hemolytic agent dosage includes a processor, a memory, and a network interface connected via a system bus. The memory includes a non-volatile storage medium and internal memory. The non-volatile storage medium stores an operating system and may also store a computer program. When executed by the processor, this computer program enables the processor to implement a method for determining insufficient hemolytic agent dosage. The internal memory may also store a computer program, which, when executed by the processor, enables the processor to implement the method for determining insufficient hemolytic agent dosage. Those skilled in the art will understand that... Figure 8 The structure shown is merely a block diagram of a portion of the structure related to the present application and does not constitute a limitation on the hemolytic agent dosage determination device applied thereto. The specific hemolytic agent dosage determination device may include more or fewer components than those shown in the figure, or combine certain components, or have different component arrangements.

[0097] A computer-readable storage medium storing a computer program, which, when executed by a processor, performs the following steps: acquiring a set of pulse signals from a blood cell sample to be tested at a preset scattering angle; identifying the signal intensity of each pulse signal in the set of pulse signals; and statistically analyzing the particle distribution of the signal intensity; wherein the particle distribution indicates the number of blood cell particles with different signal intensities, and the blood cell sample to be tested is a blood cell sample obtained after treatment with a current hemolytic agent; calculating the rate of change of the number of particles for each type of signal intensity in the particle distribution to obtain a rate of change condition; searching for a decreasing zero-crossing point in the rate of change condition, and determining a maximum particle number in the particle distribution based on the found decreasing zero-crossing point; searching for a rising zero-crossing point in the rate of change condition, and determining a minimum particle number related to red blood cells in the particle distribution based on the found rising zero-crossing point; calculating a signal interference value based on the minimum and maximum particle numbers, and if the signal interference value is greater than a preset interference threshold, determining that the current dose of the hemolytic agent is insufficient; wherein the signal interference value indicates the degree of interference of the pulse signal by red blood cells.

[0098] A device for determining insufficient hemolytic agent dosage includes a memory, a processor, and a computer program stored in the memory and executable on the processor. When the processor executes the computer program, it performs the following steps: acquiring a set of pulse signals from a blood cell sample to be tested at a preset scattering angle; identifying the signal intensity of each pulse signal in the set of pulse signals; and statistically analyzing the particle distribution of the signal intensities. The particle distribution indicates the number of blood cell particles with different signal intensities. The blood cell sample to be tested is a blood cell sample obtained after treatment with the current hemolytic agent. The device also calculates the particle distribution for each type of signal... The rate of change of the particle number intensity is used to obtain the rate of change situation; a zero-crossing point in the rate of change situation is searched, and the maximum particle number is determined in the particle distribution situation based on the searched zero-crossing point; a zero-crossing point in the rate of change situation is searched, and the minimum particle number associated with red blood cells is determined in the particle distribution situation based on the searched zero-crossing point; the signal interference value is calculated based on the minimum and maximum particle number values, and if the signal interference value is greater than a preset interference threshold, the current dose of hemolytic agent is determined to be insufficient; wherein, the signal interference value is used to indicate the degree of interference of the pulse signal by red blood cells.

[0099] It should be noted that the above-mentioned method, apparatus, device and computer-readable storage medium for determining insufficient hemolytic agent dosage belong to the same general inventive concept, and the contents of the embodiments of the method, apparatus, device and computer-readable storage medium for determining insufficient hemolytic agent dosage are applicable to each other.

[0100] Those skilled in the art will understand that all or part of the processes in the methods of the above embodiments can be implemented by a computer program instructing related hardware. This program can be stored in a non-volatile computer-readable storage medium, and when executed, it can include the processes of the embodiments of the above methods. Any references to memory, storage, databases, or other media used in the embodiments provided in this application can include non-volatile and / or volatile memory. Non-volatile memory can include read-only memory (ROM), programmable ROM (PROM), electrically programmable ROM (EPROM), electrically erasable programmable ROM (EEPROM), or flash memory. Volatile memory can include random access memory (RAM) or external cache memory. By way of illustration and not limitation, RAM is available in various forms, such as static RAM (SRAM), dynamic RAM (DRAM), synchronous DRAM (SDRAM), dual data rate SDRAM (DDRSDRAM), enhanced SDRAM (ESDRAM), synchronous link DRAM (SLDRAM), RAMbus direct RAM (RDRAM), direct memory bus dynamic RAM (DRDRAM), and RAMbus dynamic RAM (RDRAM), etc.

[0101] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0102] The above embodiments merely illustrate several implementation methods of this application, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of this patent application. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this application, and these all fall within the protection scope of this application. Therefore, the protection scope of this patent application should be determined by the appended claims.

Claims

1. A method for determining insufficient dosage of hemolytic agent, characterized in that, The method includes: The system acquires a set of pulse signals from a blood cell sample under a preset scattering angle, identifies the signal intensity of each pulse signal in the set, and statistically analyzes the particle distribution of the signal intensity. The particle distribution is used to indicate the number of blood cell particles with different signal intensities. The blood cell sample under test is a blood cell sample obtained after treatment with the current hemolytic agent. Calculate the rate of change of the number of particles for each type of signal intensity in the particle distribution to obtain the rate of change. Search for the zero-crossing point of the rate of change and determine the maximum number of particles in the particle distribution based on the searched zero-crossing point. Search for the rising zero-crossing point in the rate of change case, and determine the minimum number of particles associated with red blood cells in the particle distribution case based on the searched rising zero-crossing point; The signal interference value is calculated based on the minimum and maximum particle number values. If the signal interference value is greater than a preset interference threshold, the current dose of hemolytic agent is determined to be insufficient. The signal interference value is used to indicate the degree of interference of the pulse signal by red blood cells. The step of searching for the rising zero-crossing point in the rate of change and determining the minimum number of particles related to red blood cells in the particle distribution based on the searched rising zero-crossing point includes: Search for the first zero-crossing point in the rate of change situation, and take the number of particles corresponding to the first zero-crossing point in the particle distribution situation as the minimum value of the particle number; The calculation of the signal interference value based on the minimum and maximum particle number includes: Calculate the ratio of the minimum particle number to the maximum particle number, and use the ratio as the value of the signal being disturbed. Following the particle distribution of the statistical signal intensity, the following is also included: The particle distribution is filtered; the filtering formula is as follows: In the above formula, This shows the particle distribution after filtering. This represents the particle distribution before filtering, where i indicates the intensity of the i-th type of signal, and N indicates the total number of signal types. Indicator mean, Indicative standard deviation.

2. The method according to claim 1, characterized in that, The formula for calculating the rate of change of the particle number is: In the above formula, The rate of change of the particle number indicating the intensity of the i-th type of signal. The number of blood cell particles indicating the intensity of the (i+1)th type of signal. N indicates the number of blood cell particles representing the intensity of signal i, and N indicates the total number of signal classes.

3. The method according to claim 1, characterized in that, The process of searching for a zero-crossing point in the rate of change and determining the maximum particle number in the particle distribution based on the found zero-crossing point includes: Search for all zero-crossing points in the rate of change scenario, and obtain the number of particles corresponding to each zero-crossing point in the particle distribution scenario as candidate particle counts; The maximum value among all candidate particle counts is taken as the maximum particle count.

4. A device for determining insufficient dosage of hemolytic agent, characterized in that, The device includes: The particle distribution determination module is used to acquire the pulse signal set of the blood cell sample to be tested at a preset scattering angle, identify the signal intensity of each pulse signal in the pulse signal set, and count the particle distribution of the signal intensity; wherein, the particle distribution is used to indicate the number of blood cell particles with different signal intensities, and the blood cell sample to be tested is a blood cell sample obtained after treatment with the current hemolytic agent. The extreme point determination module is used to calculate the rate of change of the number of particles for each type of signal intensity in the particle distribution to obtain the rate of change situation; search for the falling zero-crossing point in the rate of change situation, and determine the maximum number of particles in the particle distribution based on the searched falling zero-crossing point; search for the rising zero-crossing point in the rate of change situation, and determine the minimum number of particles related to red blood cells in the particle distribution based on the searched rising zero-crossing point. The judgment module is used to calculate the signal interference value based on the minimum and maximum particle counts. If the signal interference value is greater than a preset interference threshold, it is determined that the current dose of hemolytic agent is insufficient. The signal interference value is used to indicate the degree of interference of the pulse signal by red blood cells. The extreme point determination module is also used to search for the first rising zero-crossing point in the rate of change situation, and to take the number of particles corresponding to the first rising zero-crossing point as the minimum value of the number of particles in the particle distribution situation. The judgment module is also used to calculate the ratio of the minimum particle number to the maximum particle number, and use the ratio as the interference value of the signal; The device is also used to filter the particle distribution; wherein the filtering formula is: In the above formula, This shows the particle distribution after filtering. This represents the particle distribution before filtering, where i indicates the intensity of the i-th type of signal, and N indicates the total number of signal types. Indicator mean, Indicative standard deviation.

5. A computer-readable storage medium, characterized in that, The system contains a computer program that, when executed by a processor, causes the processor to perform the steps of the method as described in any one of claims 1 to 3.

6. A device for determining insufficient dosage of hemolytic agent, characterized in that, It includes a memory and a processor, the memory storing a computer program that, when executed by the processor, causes the processor to perform the steps of the method as described in any one of claims 1 to 3.

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