Method, device, medium and equipment for judging insufficient dose of hemolytic agent

Abstract

The application discloses a kind of hemolytic agent underdosing judgment method, device, medium and equipment, by obtaining the particle distribution condition of signal intensity in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of

Description

Technical Field

[0001] This invention relates to the field of hemolytic agents, and in particular to a method, apparatus, medium, and device for determining insufficient hemolytic agent dosage. Background Technology

[0002] Blood cell analyzers can count various blood cells in the blood. For example, in one scenario, when 5LDS hemolysin is mixed with a fresh blood sample, red blood cells are dissolved and white blood cells are stained. Then, 5LHS hemolysin is added. 5LHS basic hemolysin exposes the nuclei of white blood cells except for basophils (BASO), making BASO cells significantly different in size from the other cells, thus distinguishing BASO cells.

[0003] Detection principle as follows Figure 1 As shown, the cells to be tested are arranged in a single row under the sheath fluid and flow into the flow chamber at a constant speed. Under the illumination of the laser beam, three different angles of scattered light are generated. The magnitude of the scattered light generated by the cells irradiated by the laser beam is related to the cell size, the refractive index of the cell membrane and the complexity of the internal organs of the cell. The scattered light signal is finally converted into an electrical pulse signal. Based on the collected electrical pulse data, the scatter plot distribution of white blood cells under three-dimensional signal can be obtained. Finally, the classification result of white blood cells is obtained based on the white blood cell scatter plot.

[0004] When a blood cell analyzer measures a sample, for example, if the required 5LHS hemolysing reagent is insufficient in the scenario described above, the white blood cell scatter plot will appear abnormal, leading to abnormal white blood cell classification and ultimately inaccurate cell count results. Traditional methods for detecting the remaining hemolysing reagent level involve using sensors to detect the hemolysing reagent level. However, this method requires hardware support, its principle is complex, the device is large, and due to the numerous components, a malfunction in any one component can prevent the entire detection device from functioning properly. Furthermore, sensor detection is significantly affected by interference factors, such as the most common bubble interference; the presence of bubbles may lead to a false positive for insufficient hemolysing reagent. Therefore, a simple, fast, and effective method is needed to provide an alert when the hemolysing reagent level is insufficient. Summary of the Invention

[0005] Therefore, it is necessary to provide methods, devices, media, and equipment for determining insufficient hemolytic agent dosage in order to solve the problem of inaccurate white blood cell detection caused by insufficient hemolytic agent dosage.

[0006] A method for determining insufficient dosage of hemolytic agent, the method comprising:

[0007] The system acquires a set of pulse signals from a blood cell sample under a preset scattering angle, identifies the signal intensity of each pulse signal in the set, and statistically analyzes the particle distribution of the signal intensity. The particle distribution is used to indicate the number of blood cell particles with different signal intensities. The blood cell sample under test is a blood cell sample obtained after treatment with the current hemolytic agent.

[0008] Calculate the rate of change of the number of particles for each type of signal intensity in the particle distribution to obtain the rate of change.

[0009] Search for the zero-crossing point of the rate of change, and determine the local maximum of the particle number in the particle distribution based on the searched zero-crossing point.

[0010] If the number of local maxima of the particle count is greater than 1, then the current dose of hemolytic agent is determined to be severely insufficient.

[0011] If the number of local maxima of the particle population is equal to 1, then calculate the mean and standard deviation of the number of different signal intensities, and calculate the kurtosis coefficient based on the mean and standard deviation of the number of times.

[0012] If the kurtosis coefficient is less than a preset threshold, the current hemolytic agent dose is determined to be slightly insufficient; if the kurtosis coefficient is greater than or equal to the preset threshold, the current hemolytic agent dose is determined to be sufficient.

[0013] In one embodiment, the formula for calculating the particle number change rate is:

[0014] S i =La i+1 -La i (i∈1,2,3,…N-1)

[0015] In the above formula, S i La indicates the rate of change of the particle population indices of the intensity of the i-th type of signal. i+1 The number of blood cell particles, La, indicating the intensity of the (i+1)th type of signal. i N indicates the number of blood cell particles representing the intensity of signal i, and N indicates the total number of signal classes.

[0016] In one embodiment, the step of searching for a zero-crossing point in the rate of change and determining a local maximum of the particle number in the particle distribution based on the found zero-crossing point includes:

[0017] Search for all zero-crossing points in the rate of change case, and obtain the number of particles corresponding to each zero-crossing point in the particle distribution case as the local maximum of the particle number.

[0018] In one embodiment, the formula for calculating the average number of occurrences is:

[0019]

[0020] In the above formula, La i The number of particles indicating the intensity of the i-th type of signal, where N is the total number of signal intensity categories, and La mean Indicates the average of the stated times;

[0021] The formula for calculating the standard deviation of the frequency is:

[0022]

[0023] In the above formula, La sd Indicates the standard deviation of the stated frequency.

[0024] In one embodiment, the formula for calculating the kurtosis coefficient is:

[0025]

[0026] In the above formula, δ Kurtosis is the kurtosis coefficient.

[0027] In one embodiment, after the particle distribution of the statistical signal intensity, the method further includes:

[0028] The particle distribution is filtered; the filtering formula is as follows:

[0029]

[0030] In the above formula, F(Ful i The image shows the particle distribution after filtering. i The particle distribution before filtering is represented by i, where i indicates the intensity of the i-th type of signal, N indicates the total number of signal types, u indicates the mean, and σ indicates the standard deviation.

[0031] A device for determining insufficient dosage of hemolytic agent, the device comprising:

[0032] The particle distribution determination module is used to acquire the pulse signal set of the blood cell sample to be tested at a preset scattering angle, identify the signal intensity of each pulse signal in the pulse signal set, and count the particle distribution of the signal intensity; wherein, the particle distribution is used to indicate the number of blood cell particles with different signal intensities, and the blood cell sample to be tested is a blood cell sample obtained after treatment with the current hemolytic agent.

[0033] The extreme point determination module is used to calculate the rate of change of the number of particles for each type of signal intensity in the particle distribution to obtain the rate of change situation; search for the zero-crossing point of the rate of change situation, and determine the local maximum of the number of particles in the particle distribution based on the searched zero-crossing point.

[0034] The judgment module is used to determine that if the number of local maxima of the particle count is greater than 1, the current hemolytic agent dose is seriously insufficient; if the number of local maxima of the particle count is equal to 1, the mean and standard deviation of the number of different signal intensities are calculated, and the kurtosis coefficient is calculated based on the mean and standard deviation of the number of times; if the kurtosis coefficient is greater than a preset threshold, the current hemolytic agent dose is slightly insufficient; if the kurtosis coefficient is less than or equal to the preset threshold, the current hemolytic agent dose is sufficient.

[0035] A computer-readable storage medium storing a computer program, which, when executed by a processor, causes the processor to perform the steps of the above-described method for determining insufficient hemolytic agent dosage.

[0036] A device for determining insufficient hemolytic agent dosage includes a memory and a processor. The memory stores a computer program, which, when executed by the processor, causes the processor to perform the steps of the aforementioned method for determining insufficient hemolytic agent dosage.

[0037] This invention provides a method, apparatus, medium, and device for determining insufficient hemolytic agent dosage. It acquires a set of pulse signals from a blood cell sample at a preset scattering angle and statistically analyzes the particle distribution of the signal intensity. Then, it calculates the rate of change of particle count for each signal intensity type within the particle distribution, searches for a zero-crossing point in the rate of change, and determines local maxima of particle count based on these zero-crossing points. This allows for determination of whether the current hemolytic agent dosage is insufficient. If the number of local maxima is greater than 1, the current hemolytic agent dosage is considered severely insufficient; if the number of local maxima is equal to 1, the kurtosis coefficient is calculated to determine whether the hemolytic agent dosage is slightly insufficient or sufficient. Therefore, this invention can monitor hemolytic agent dosage based on local maxima and kurtosis coefficients, reducing instrument detection costs, simplifying instrument construction, and ensuring sufficient accuracy because it is not affected by interference factors such as air bubbles. Attached Figure Description

[0038] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0039] in:

[0040] Figure 1 This is a schematic diagram illustrating the principle of white blood cell monitoring.

[0041] Figure 2 A flowchart illustrating the method for determining insufficient hemolytic agent dosage;

[0042] Figure 3 A schematic diagram illustrating the generation of scattered light at three different angles;

[0043] Figure 4 A scatter plot of signal intensity and particle distribution of leukocytes under conditions of severe hemolytic agent deficiency;

[0044] Figure 5 for Figure 4 A schematic diagram of the local maxima of the particle number in the particle distribution;

[0045] Figure 6 Scatter plot of signal intensity and particle distribution of white blood cells under conditions of slight hemolytic agent deficiency;

[0046] Figure 7 A scatter plot of signal intensity and particle distribution of leukocytes under conditions of sufficient hemolytic agent;

[0047] Figure 8 This is a schematic diagram of a device for determining insufficient hemolytic agent dosage.

[0048] Figure 9 This is a block diagram of a device for determining insufficient hemolytic agent dosage. Detailed Implementation

[0049] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0050] The terms "first," "second," etc., in the specification, claims, and accompanying drawings of this application are used to distinguish different objects, not to describe a specific order. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion. For example, a process, method, system, product, or apparatus that includes a series of steps or units is not limited to the listed steps or units, but may optionally include steps or units not listed, or may optionally include other steps or units inherent to these processes, methods, products, or apparatuses.

[0051] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0052] like Figure 2 As shown, Figure 2 This is a flowchart illustrating a method for determining insufficient hemolytic agent dosage in one embodiment. The steps provided by the method for determining insufficient hemolytic agent dosage in this embodiment include:

[0053] S201, acquire the pulse signal set of the blood cell sample to be tested at a preset scattering angle, identify the signal intensity of each pulse signal in the pulse signal set, and count the particle distribution of the signal intensity.

[0054] The particle distribution is used to indicate the number of blood cell particles with different signal intensities, and the blood cell sample to be tested is a blood cell sample obtained after treatment with the current hemolytic agent.

[0055] For example, in one scenario, the blood cell sample to be tested is first treated with sufficient 5LDS hemolysin, after which red blood cells are lysed and white blood cells are stained. Then, the current 5LHS hemolysin is added. The 5LHS basic hemolysin exposes the nuclei of white blood cells except for basophils (BASO), making BASO cells significantly different in size from the other cells, thus obtaining white blood cell and red blood cell fragments. Enveloped in sheath fluid, the cells are arranged in a single row and flow into the flow chamber at a uniform speed. Under the irradiation of the laser beam, as referenced... Figure 3 This generates scattered light at three different angles: low-angle, medium-angle, and high-angle scattered light. Low-angle scattered light refers to light scattered from the forward low-angle region, medium-angle scattered light from the forward medium-angle region, and high-angle scattered light from the side high-angle region. Low-angle scattered light reflects cell size, medium-angle scattered light reflects the fine internal structure and particulate matter of the cell, and high-angle forward scattered light also reflects the fine internal structure and particulate matter of the cell. An aperture in the receiving section is used to determine the presence of scattered light. The first receiver receives the medium-angle scattered light emitted from the flow chamber and converts it into a medium-angle pulse signal, forming the pulse signal set corresponding to the medium angle. The second receiver receives the high-angle scattered light emitted from the flow chamber and converts it into a high-angle pulse signal, forming the pulse signal set corresponding to the high angle. The third receiver receives the low-angle scattered light emitted from the flow chamber and converts it into a low-angle pulse signal, forming the pulse signal set corresponding to the low angle. In this embodiment, any one of the pulse signal sets at a preset scattering angle can be selected for subsequent judgment.

[0056] Furthermore, existing pulse recognition algorithms are used to identify the signal strength of each pulse signal in the pulse signal set, such as threshold detection algorithms or energy threshold algorithms. The signal strengths of all pulse signals are then summarized to obtain Rp. a , (a∈1,2,3,…A), represents the signal intensity of the pulse signal of the a-th white blood cell in the sample, where A represents the total number of pulse signals.

[0057] Furthermore, based on these signal intensities, a scatter plot of the signal intensities of white blood cells in three dimensions can be obtained. For example, as shown... Figure 4 As shown in (a), the Y-axis (LS) represents the signal intensity of low-angle scattered light, and the X-axis (MS) represents the signal intensity of mid-angle scattered light. Of course, this scatter plot can also be constructed based on scattered light from other angles.

[0058] Furthermore, the particle distribution of the statistical signal intensity is used to indicate the number of blood cell particles with different signal intensities, where La i , (i = 1, 2, 3, ... N) indicates the number of particles of the i-th type of signal strength, and N is the total number of signal strength types. For example, corresponding to Figure (4), Figure 4 The scatter plot in (a) can be mapped along the LS direction to obtain... Figure 4 (b) shows the particle distribution.

[0059] In one specific embodiment, the particle distribution is further filtered; wherein the filtering formula is:

[0060]

[0061] In the above formula, F(Ful i The image shows the particle distribution after filtering. i The particle distribution before filtering is represented by i, where i indicates the intensity of the i-th type of signal, N indicates the total number of signal types, u indicates the mean, and σ indicates the standard deviation.

[0062] After the above filtering process, noise in the particle distribution can be effectively removed, making the overall distribution smoother.

[0063] S202, calculate the rate of change of the number of particles for each type of signal intensity in the particle distribution to obtain the rate of change.

[0064] Based on this rate of change, we can understand the trend of particle number changes at each signal intensity in the particle distribution.

[0065] In one specific embodiment, the formula for calculating the particle number change rate of the i-th type of signal intensity is:

[0066] S i =La i+1 -La i (i∈1,2,3,…N-1)

[0067] In the above formula, S i La indicates the rate of change of the particle population indices of the intensity of the i-th type of signal. i+1 The number of blood cell particles, La, indicating the intensity of the (i+1)th type of signal. i N indicates the number of blood cell particles representing the intensity of signal i, and N indicates the total number of signal classes.

[0068] S203, search for the zero-crossing point of the rate of change, and determine the local maximum of the particle number in the particle distribution based on the searched zero-crossing point.

[0069] In the case of rate of change, a local range to the left of the zero-crossing point indicates that the number of particles with increasing signal strength in the corresponding particle distribution is continuously increasing; a local range to the right of the zero-crossing point indicates that the number of particles with increasing signal strength in the corresponding particle distribution is continuously decreasing. Based on these conditions, the zero-crossing point can be found in the case of rate of change. Optionally, the zero-crossing point can be defined as the rate of change S of the number of particles for a signal strength of type i. i In general, a point is considered to be a zero-crossing point of descent if the following conditions are met:

[0070]

[0071] Then, based on these zero-crossing points of decline that have been found, the local maxima of the particle number can be determined in the particle distribution.

[0072] In one specific embodiment, the local maximum of the particle number is determined by searching all zero-crossing points in the rate of change and obtaining the particle number corresponding to each zero-crossing point in the particle distribution as the local maximum of the particle number. For example, in... Figure 5 In the process, the number of particles corresponding to A, B, and C is determined as the local maximum of the particle number.

[0073] S204, compare the number of local maxima of particle count with the value of 1. If the number of local maxima of particle count is greater than 1, then execute S205, determining that the current hemolytic agent dosage is severely insufficient. If the number of local maxima of particle count is equal to 1, then execute S206.

[0074] For example, such as Figure 5As shown, if the number of local maxima of particle number is 3, which is greater than 1, then the current dose of hemolytic agent is determined to be severely insufficient.

[0075] This is because, in one scenario, when 5LDS hemolysin is mixed with a fresh blood sample, red blood cells are dissolved while white blood cells are stained. Then, 5LHS hemolysin is added. The basic 5LHS hemolysin neutralizes the nuclei of all white blood cells except basophils (BASO), making BASO cells distinct in size from the rest of the cells, thus allowing BASO cells to be differentiated. If the required 5LHS hemolysin is severely insufficient in this scenario, the nuclei of all white blood cells except BASO will not be completely neutralized. Figure 4 (a) The scatter plot will show many abnormal points (points within the box area) other than those representing basophils. After mapping, Figure 4 (b) The particle distribution will show multiple local maxima of particle number (excessive local maxima of particle number appear in the elliptical region).

[0076] Conversely, if the number of local maxima of particles is equal to 1, then it indicates that... Figure 4 In this specific embodiment, the dosage of hemolysin used in the blood cell sample to be tested was not severely insufficient, and further precise determination can be made through subsequent steps. For example, [the dosage of hemolysin in the sample is not specified]. Figure 6 The scatter plot in (a) is obtained by mapping it along the LS direction. Figure 6 (b), because Figure 6 If the number of local maxima of particle number in (b) is equal to 1, it is determined that the current dose of hemolytic agent is not seriously insufficient, and S206 is further executed.

[0077] S206, calculate the mean and standard deviation of the number of times for different signal strengths, and calculate the kurtosis coefficient based on the mean and standard deviation of the number of times.

[0078] The formula for calculating the mean of the frequencies is as follows:

[0079]

[0080] In the above formula, La i The number of particles indicating the intensity of the i-th type of signal, where N is the total number of signal intensity categories, and La mean The average number of times indicated.

[0081] The formula for calculating the standard deviation of the frequency is:

[0082]

[0083] In the above formula, La sd Standard deviation of the indicated frequency.

[0084] The formula for calculating the kurtosis coefficient is as follows:

[0085]

[0086] In the above formula, δ Kurtosis The kurtosis coefficient is a statistic used to describe the kurtosis of a probability distribution. It is an indicator that describes the flatness or arching of the probability distribution and characterizes the kurtosis and sharpness of the random variable distribution.

[0087] S207, determine if the kurtosis coefficient is less than a preset threshold. If the kurtosis coefficient is less than the preset threshold, proceed to S208, determining that the current hemolytic agent dose is slightly insufficient. If the kurtosis coefficient is greater than or equal to the preset threshold, proceed to S209, determining that the current hemolytic agent dose is sufficient.

[0088] If δ Kurtosis If δ is greater than the threshold Th, it indicates that the particle distribution is sharper and the peaks are narrower than a normal distribution; if δ Kurtosis If δ equals the threshold Th, it indicates that the particle distribution has the same shape as the normal distribution. Kurtosis If the value is less than the threshold Th, it indicates that the particle distribution is more gradual and the peaks are wider than the normal distribution.

[0089] For example, after setting an appropriate threshold, Figure 6 The kurtosis coefficient corresponding to the specific embodiment is less than the threshold, therefore it is determined that... Figure 6 In this specific embodiment, the dosage of hemolysing agent used in the blood cell sample was slightly insufficient. This is because if the required 5LHS hemolysing agent is slightly insufficient in this scenario, then naked leukocytes, except for basophils (BASO), will not be completely nucleated. Figure 6 (a) A small number of abnormal points (points within the box area) will appear in the scatter plot, other than those representing basophils. After mapping, Figure 6 (b) The particle distribution will show additional flat regions (elliptical regions).

[0090] For example, after setting an appropriate threshold, Figure 7 The kurtosis coefficient corresponding to the specific embodiment is greater than the threshold, therefore it is determined that... Figure 7 In this specific embodiment, the dosage of hemolysing agent used in the blood cell sample to be tested is sufficient. This is because if the required 5LHS hemolysing reagent is sufficient in this scenario, then all naked white blood cells except basophils (BASO) will be completely nucleated. Figure 7 (a) The scatter plot will not show a small number of abnormal points other than those representing basophils; only one cluster (points within the box area) will appear. After mapping, Figure 7 (b) The particle distribution will only show one sharp peak (elliptical region).

[0091] The aforementioned method for determining insufficient hemolytic agent dosage involves acquiring a set of pulse signals from a blood cell sample at a preset scattering angle and statistically analyzing the particle distribution of the signal intensity. It then calculates the rate of change of particle count for each signal intensity type within the particle distribution, searches for a zero-crossing point in the rate of change, and determines local maxima of particle count based on these zero-crossing points. This allows for the determination of whether the current hemolytic agent dosage is insufficient. If the number of local maxima is greater than 1, the current hemolytic agent dosage is considered severely insufficient; if the number of local maxima is equal to 1, the kurtosis coefficient is calculated to determine whether the hemolytic agent dosage is slightly insufficient or sufficient. Therefore, this invention can monitor hemolytic agent dosage based on local maxima and kurtosis coefficients, reducing instrument detection costs, simplifying instrument construction, and ensuring sufficient accuracy as it is not affected by interference factors such as air bubbles.

[0092] In one embodiment, such as Figure 8 As shown, a device for determining insufficient hemolytic agent dosage is proposed, the device comprising:

[0093] The particle distribution determination module 801 is used to acquire the pulse signal set of the blood cell sample to be tested at a preset scattering angle, identify the signal intensity of each pulse signal in the pulse signal set, and count the particle distribution of the signal intensity; wherein, the particle distribution is used to indicate the number of blood cell particles with different signal intensities, and the blood cell sample to be tested is a blood cell sample obtained after treatment with the current hemolysing agent.

[0094] The extreme point determination module 802 is used to calculate the rate of change of the number of particles for each type of signal intensity in the particle distribution to obtain the rate of change situation; search for the zero-crossing point of the rate of change situation, and determine the local maximum of the number of particles in the particle distribution based on the searched zero-crossing point of the rate of change.

[0095] The judgment module 803 is used to determine whether the current hemolytic agent dose is seriously insufficient if the number of local maxima of particle count is greater than 1; if the number of local maxima of particle count is equal to 1, it calculates the mean and standard deviation of the number of different signal intensities, and calculates the kurtosis coefficient based on the mean and standard deviation of the number of counts; if the kurtosis coefficient is greater than a preset threshold, it determines that the current hemolytic agent dose is slightly insufficient; if the kurtosis coefficient is less than or equal to the preset threshold, it determines that the current hemolytic agent dose is sufficient.

[0096] In one embodiment, the formula for calculating the rate of change of particle number is:

[0097] S i =La i+1 -La i (i∈1,2,3,…N-1)

[0098] In the above formula, Si La indicates the rate of change of the particle population indices of the intensity of the i-th type of signal. i+1 The number of blood cell particles, La, indicating the intensity of the (i+1)th type of signal. i N indicates the number of blood cell particles representing the intensity of signal i, and N indicates the total number of signal classes.

[0099] In one embodiment, the extreme point determination module 802 is specifically used for:

[0100] Search for all zero-crossing points in the rate of change case, and obtain the number of particles corresponding to each zero-crossing point in the particle distribution case as a local maximum of the particle number.

[0101] In one embodiment, the formula for calculating the mean of the number of occurrences is:

[0102]

[0103] In the above formula, La i The number of particles indicating the intensity of the i-th type of signal, where N is the total number of signal intensity categories, and La mean Mean of the number of indications;

[0104] The formula for calculating the standard deviation of frequencies is:

[0105]

[0106] In the above formula, La sd Standard deviation of the indicated frequency.

[0107] In one embodiment, the formula for calculating the kurtosis coefficient is:

[0108]

[0109] In the above formula, δ Kurtosis This represents the kurtosis coefficient.

[0110] In one embodiment, the device for determining insufficient hemolytic agent dosage is further used for:

[0111] The particle distribution is filtered; the filtering formula is as follows:

[0112]

[0113] In the above formula, F(Ful i The image shows the particle distribution after filtering. i The particle distribution before filtering is represented by i, where i indicates the intensity of the i-th type of signal, N indicates the total number of signal types, u indicates the mean, and σ indicates the standard deviation.

[0114] Figure 9An internal structural diagram of a device for determining insufficient hemolytic agent dosage is shown in one embodiment. Figure 9 As shown, the device for determining insufficient hemolytic agent dosage includes a processor, a memory, and a network interface connected via a system bus. The memory includes a non-volatile storage medium and internal memory. The non-volatile storage medium stores an operating system and may also store a computer program. When executed by the processor, this computer program enables the processor to implement a method for determining insufficient hemolytic agent dosage. The internal memory may also store a computer program, which, when executed by the processor, enables the processor to implement the method for determining insufficient hemolytic agent dosage. Those skilled in the art will understand that... Figure 9 The structure shown is merely a block diagram of a portion of the structure related to the present application and does not constitute a limitation on the hemolytic agent dosage determination device applied thereto. The specific hemolytic agent dosage determination device may include more or fewer components than those shown in the figure, or combine certain components, or have different component arrangements.

[0115] A computer-readable storage medium storing a computer program, which, when executed by a processor, performs the following steps: acquiring a set of pulse signals from a blood cell sample to be tested at a preset scattering angle; identifying the signal intensity of each pulse signal in the set of pulse signals; and statistically analyzing the particle distribution of the signal intensities; wherein the particle distribution indicates the number of blood cell particles with different signal intensities; the blood cell sample to be tested is a blood cell sample obtained after treatment with a current hemolysin; and calculating the rate of change of the number of particles for each type of signal intensity in the particle distribution to obtain the rate of change. The system searches for a zero-crossing point in the rate of change and determines the local maximum of the number of particles in the particle distribution based on the found zero-crossing point. If the number of local maxima of the number of particles is greater than 1, the current hemolytic agent dose is determined to be severely insufficient. If the number of local maxima of the number of particles is equal to 1, the mean and standard deviation of the number of different signal intensities are calculated, and the kurtosis coefficient is calculated based on the mean and standard deviation of the number of times. If the kurtosis coefficient is less than a preset threshold, the current hemolytic agent dose is determined to be slightly insufficient. If the kurtosis coefficient is greater than or equal to the preset threshold, the current hemolytic agent dose is determined to be sufficient.

[0116] A device for determining insufficient hemolytic agent dosage includes a memory, a processor, and a computer program stored in the memory and executable on the processor. When the processor executes the computer program, it performs the following steps: acquiring a set of pulse signals from a blood cell sample to be tested at a preset scattering angle; identifying the signal intensity of each pulse signal in the set of pulse signals; and statistically analyzing the particle distribution of the signal intensities. The particle distribution indicates the number of blood cell particles with different signal intensities. The blood cell sample to be tested is a blood cell sample obtained after treatment with the current hemolytic agent. The device also calculates the change in the number of particles for each type of signal intensity in the particle distribution. The rate of change is determined; a zero-crossing point in the rate of change is searched, and a local maximum of the number of particles is determined in the particle distribution based on the searched zero-crossing point; if the number of local maxima of the number of particles is greater than 1, the current hemolytic agent dose is determined to be severely insufficient; if the number of local maxima of the number of particles is equal to 1, the mean and standard deviation of the number of different signal intensities are calculated, and the kurtosis coefficient is calculated based on the mean and standard deviation of the number of times; if the kurtosis coefficient is less than a preset threshold, the current hemolytic agent dose is determined to be slightly insufficient; if the kurtosis coefficient is greater than or equal to the preset threshold, the current hemolytic agent dose is determined to be sufficient.

[0117] It should be noted that the above-mentioned method, apparatus, device and computer-readable storage medium for determining insufficient hemolytic agent dosage belong to the same general inventive concept, and the contents of the embodiments of the method, apparatus, device and computer-readable storage medium for determining insufficient hemolytic agent dosage are applicable to each other.

[0118] Those skilled in the art will understand that all or part of the processes in the methods of the above embodiments can be implemented by a computer program instructing related hardware. This program can be stored in a non-volatile computer-readable storage medium, and when executed, it can include the processes of the embodiments of the above methods. Any references to memory, storage, databases, or other media used in the embodiments provided in this application can include non-volatile and / or volatile memory. Non-volatile memory may include read-only memory (ROM), programmable ROM (PROM), electrically programmable ROM (EPROM), electrically erasable programmable ROM (EEPROM), or flash memory. Volatile memory may include random access memory (RAM) or external cache memory. By way of illustration and not limitation, RAM is available in various forms, such as static RAM (SRAM), dynamic RAM (DRAM), synchronous DRAM (SDRAM), dual data rate SDRAM (DDRSDRAM), enhanced SDRAM (ESDRAM), synchronous link DRAM (SLDRAM), RAMbus direct RAM (RDRAM), direct memory bus dynamic RAM (DRDRAM), and RAMbus dynamic RAM (RDRAM), etc.

[0119] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0120] The above embodiments merely illustrate several implementation methods of this application, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of this patent application. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this application, and these all fall within the protection scope of this application. Therefore, the protection scope of this patent application should be determined by the appended claims.

Claims

1. A method for determining insufficient dosage of hemolytic agent, characterized in that, The method includes: The system acquires a set of pulse signals from a blood cell sample under a preset scattering angle, identifies the signal intensity of each pulse signal in the set, and statistically analyzes the particle distribution of the signal intensity. The particle distribution is used to indicate the number of blood cell particles with different signal intensities. The blood cell sample under test is a blood cell sample obtained after treatment with the current hemolytic agent. Calculate the rate of change of the number of particles for each type of signal intensity in the particle distribution to obtain the rate of change. Search for the zero-crossing point of the rate of change, and determine the local maximum of the particle number in the particle distribution based on the searched zero-crossing point. If the number of local maxima of the particle count is greater than 1, then the current dose of hemolytic agent is determined to be severely insufficient. If the number of local maxima of the particle population is equal to 1, then calculate the mean and standard deviation of the number of different signal intensities, and calculate the kurtosis coefficient based on the mean and standard deviation of the number of times. If the kurtosis coefficient is less than a preset threshold, the current hemolytic agent dose is determined to be slightly insufficient; if the kurtosis coefficient is greater than or equal to the preset threshold, the current hemolytic agent dose is determined to be sufficient.

2. The method according to claim 1, characterized in that, The formula for calculating the rate of change of the particle number is: S i =The i+1 -The i ,(i∈1,2,3,…N-1) In the above formula, S i La indicates the rate of change of the particle population indices of the intensity of the i-th type of signal. i+1 The number of blood cell particles, La, indicating the intensity of the (i+1)th type of signal. i N indicates the number of blood cell particles representing the intensity of signal i, and N indicates the total number of signal classes.

3. The method according to claim 1, characterized in that, The process of searching for a zero-crossing point in the rate of change and determining a local maximum of the particle number in the particle distribution based on the found zero-crossing point includes: Search for all zero-crossing points in the rate of change case, and obtain the number of particles corresponding to each zero-crossing point in the particle distribution case as the local maximum of the particle number.

4. The method according to claim 1, characterized in that, The formula for calculating the average number of occurrences is: In the above formula, La i The number of particles indicating the intensity of the i-th type of signal, where N is the total number of signal intensity categories, and La mean Indicates the average of the stated times; The formula for calculating the standard deviation of the frequency is: In the above formula, La sd Indicates the standard deviation of the stated frequency.

5. The method according to claim 4, characterized in that, The formula for calculating the kurtosis coefficient is as follows: In the above formula, δ Kurtosis is the kurtosis coefficient.

6. The method according to claim 1, characterized in that, Following the particle distribution of the statistical signal intensity, the following is also included: The particle distribution is filtered; the filtering formula is as follows: In the above formula, F(Ful i The image shows the particle distribution after filtering. i The particle distribution before filtering is represented by i, where i indicates the intensity of the i-th type of signal, N indicates the total number of signal types, u indicates the mean, and σ indicates the standard deviation.

7. A device for determining insufficient dosage of hemolytic agent, characterized in that, The device includes: The particle distribution determination module is used to acquire the pulse signal set of the blood cell sample to be tested at a preset scattering angle, identify the signal intensity of each pulse signal in the pulse signal set, and count the particle distribution of the signal intensity; wherein, the particle distribution is used to indicate the number of blood cell particles with different signal intensities, and the blood cell sample to be tested is a blood cell sample obtained after treatment with the current hemolytic agent. The extreme point determination module is used to calculate the rate of change of the number of particles for each type of signal intensity in the particle distribution to obtain the rate of change situation; search for the zero-crossing point of the rate of change situation, and determine the local maximum of the number of particles in the particle distribution based on the searched zero-crossing point. The judgment module is used to determine that if the number of local maxima of the particle count is greater than 1, the current hemolytic agent dose is seriously insufficient; if the number of local maxima of the particle count is equal to 1, the mean and standard deviation of the number of different signal intensities are calculated, and the kurtosis coefficient is calculated based on the mean and standard deviation of the number of times; if the kurtosis coefficient is greater than a preset threshold, the current hemolytic agent dose is slightly insufficient; if the kurtosis coefficient is less than or equal to the preset threshold, the current hemolytic agent dose is sufficient.

8. A computer-readable storage medium, characterized in that, The system stores a computer program that, when executed by a processor, causes the processor to perform the steps of the method as described in any one of claims 1 to 6.

9. A device for determining insufficient dosage of hemolytic agent, characterized in that, It includes a memory and a processor, the memory storing a computer program that, when executed by the processor, causes the processor to perform the steps of the method as described in any one of claims 1 to 6.

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