A method for inducing coral planktonic larvae to improve their attachment ability

Abstract

This invention discloses a method for inducing coral planktonic larvae to improve their attachment ability in the field of planktonic larvae induction technology, comprising the following steps: Step 1, cutting shell-like coral algae to a certain particle size to obtain shell-like coral algae particles, and then filling the shell-like coral algae particles into porous shell-like coral algae to obtain an induction culture substrate; Step 2, placing planktonic larvae on the induction culture substrate, then placing them in an induction culture container, and immersing them in seawater at a temperature of 27°C for 2-7 days; Step 3, keeping the seawater warm through the induction culture container, and after 2-7 days, raising the temperature of the seawater through the induction culture container to continue the induction culture. This invention improves the temperature and induction structure under the condition of utilizing existing induction substrates, thereby enhancing the attachment ability of planktonic larvae.

Description

Technical Field

[0001] This invention belongs to the field of coral planktonic larvae induction technology, specifically a method for inducing coral planktonic larvae to improve their attachment ability. Background Technology

[0002] Coral reef ecosystems are the most productive natural ecosystems in the world's oceans. Coral reefs around the world are degrading rapidly, and restoration to rebuild coral reefs has become an urgent priority for global ecological protection. The reproduction of reef-building coral populations is mainly accomplished through sexual reproduction, and the survival and successful attachment of coral planktonic larvae are key ecological processes for the successful replenishment of the population.

[0003] The paper "Influence of Coral Algae on the Acoustic Field and Attachment of Planktonic Larvae of *Symplocos rubrum*" proposes that shell-shaped coral algae have a significant inducing effect on the attachment of planktonic larvae, and can significantly improve the attachment ability of planktonic larvae. Meanwhile, Chinese Patent Publication No. CN 110036947 B relates to a method for inducing increased attachment ability in coral planktonic larvae. This method includes the following steps: using live shell-shaped coral algae as the larval attachment culture medium substrate; uniformly spraying brompyrrole alkaloid (-)-Agelastatin A polycaprolactone microspheres onto the surface of the shell-shaped coral algae; or applying an organic solvent solution of brompyrrole alkaloid (-)-Agelastatin A onto the live shell-shaped coral algae; placing the coral planktonic larvae on the substrate of the culture container formed by the above treatment, and cultivating them in seawater. This invention also provides brompyrrole alkaloid polycaprolactone microspheres and their preparation method. This invention uses live shell-shaped coral algae as the substrate for larval attachment culture, and adds polycaprolactone microspheres containing bromide pyrrole alkaloid (-)-Agelastatin A. This effectively promotes the attachment and development of coral planktonic larvae on the substrate, thereby improving the ecological conservation level of corals and the ecological restoration technology level of coral reefs.

[0004] The above studies clearly show that using shell-shaped coral algae as a culture substrate has a significant effect on improving the attachment ability of planktonic larvae. However, there are relatively few studies on the effect of changing other conditions on improving the attachment ability of planktonic larvae. Summary of the Invention

[0005] The purpose of this invention is to provide a method for inducing coral planktonic larvae to improve their attachment ability, so as to further improve the ability of shell-shaped coral algae to induce the attachment of planktonic larvae based on the prior art.

[0006] To achieve the above objectives, the technical solution of the present invention is as follows: A method for inducing coral planktonic larvae to improve their attachment ability, comprising the following steps:

[0007] Step 1: Cut the shell-shaped coral algae to a certain size to obtain shell-shaped coral algae particles, and then fill the shell-shaped coral algae particles into the porous shell-shaped coral algae to obtain the induction culture medium base.

[0008] Step 2: Place the planktonic larvae on the bottom of the induction culture medium, then put them into the induction culture container and place them in seawater at a temperature of 27°C for 2-7 days of induction culture;

[0009] Step 3: Keep the seawater warm using an induction culture container, and after 2-7 days, raise the temperature of the seawater using the induction culture container to continue the induction culture.

[0010] Furthermore, in step 1, the filling rate reaches 80-95%.

[0011] Furthermore, in step 2, the planktonic larvae are placed inside the holes.

[0012] Furthermore, the induction culture container includes a main body with an opening at the top, a cross-shaped partition fixedly installed inside the main body, the cross-shaped partition dividing the main body into several culture chambers, a support fixedly installed on the cross-shaped partition, several nozzles installed on the support, each nozzle corresponding to and connected to a culture chamber, each culture chamber being connected to an outlet valve, and also includes a pump assembly, the input end of the pump assembly being connected to a reversing valve, one inlet of the reversing valve being connected to a seawater source, the other inlet of the reversing valve being connected to the outlet valve through a pipe, the output end of the pump assembly being connected to a heater, the heater being connected to several nozzles through a water pipe.

[0013] Furthermore, the nozzle is a flow-regulating nozzle.

[0014] Furthermore, a heat insulation layer is fixedly installed on the side wall of the main body.

[0015] Furthermore, in step 3, during the induction culture, the pump assembly and the outlet valve are connected by a reversing valve, and the pump assembly circulates the water in the culture chamber.

[0016] Furthermore, in step 3, during the induction culture, the seawater in the culture chamber is made to fluctuate through the liquid outlet valve and nozzle to simulate the ocean wave environment.

[0017] Furthermore, a support plate is provided inside the culture chamber and is vertically slidably connected to the main body. An electric control cylinder is provided below the support plate. The electric control cylinder is located outside the main body, and the output shaft of the electric control cylinder is fixedly connected to the bottom of the support plate.

[0018] The technical principle and beneficial effects of the above scheme are as follows: (1) In this invention, the shell coral algae with holes is used as the supporting skeleton, and the cut shell coral algae is used as the filling material. In this way, the area that the planktonic larvae can contact the shell coral algae is increased, so that the beneficial microbial population of the shell coral algae can induce the planktonic larvae to improve their attachment ability.

[0019] (2) In this invention, the seawater temperature is selected as 27°C. The purpose is to ensure that the survival rate of planktonic larvae is high at this temperature, so as to ensure the stable development of planktonic larvae. Furthermore, the use of seawater is more conducive to subsequent marine ecological restoration. At the same time, when the seawater temperature is raised to 30°C, the activity of planktonic larvae can be promoted after the seawater temperature is raised, thereby improving the attachment ability of planktonic larvae.

[0020] (3) In this invention, by using an induction container and controlling the pump assembly and reversing valve, the seawater in the culture chamber can be circulated and sprayed out from the top of the culture chamber through the nozzle. On the one hand, the oxygen content in the seawater can be increased and the problem of seawater acidification can be reduced. This can improve the attachment ability of planktonic larvae to a certain extent. On the other hand, the seawater enters the culture chamber through the nozzle, so that the seawater can form corresponding waves, similar to the marine environment.

[0021] In summary, this application modifies the structure of the existing induction culture substrate to increase the induction area, and also modifies the temperature to enhance the attachment ability of induced planktonic larvae. Furthermore, it ensures environmental stability and simulates the seawater environment to a certain extent, which is beneficial to the survival of planktonic larvae.

[0022] Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0023] Figure 1 This is a flowchart illustrating the method of inducing coral planktonic larvae to improve their attachment ability according to the present invention.

[0024] Figure 2 This is a schematic diagram of the structure of the induction culture container of the present invention;

[0025] Figure 3 This is a cross-sectional view of the induction culture container of the present invention. Detailed Implementation

[0026] Embodiments of the present invention are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0027] In the description of this invention, it should be understood that the terms "longitudinal", "lateral", "vertical", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", and "outer" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0028] In the description of this invention, unless otherwise specified and limited, it should be noted that the terms "installation", "connection" and "linking" should be interpreted broadly. For example, they can refer to mechanical or electrical connections, or internal connections between two components. They can be direct connections or indirect connections through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms according to the specific circumstances.

[0029] The following detailed description illustrates the specific implementation method:

[0030] The reference numerals in the accompanying drawings include: directional valve 11, pipe 12, liquid outlet valve 13, baffle 14, nozzle 15, support 16, culture chamber 17, heater 18, pump assembly 19, and electric control cylinder 21.

[0031] This application provides a method for inducing coral planktonic larvae to improve their attachment ability, as shown in the attached... Figures 1-3 As shown: It includes the following steps:

[0032] Step 1: Cut the shell-shaped coral algae to a certain particle size to obtain shell-shaped coral algae particles. Then, fill the porous shell-shaped coral algae with the particles to obtain the induction culture medium substrate, with a filling rate of 80-95%. In this embodiment, the particle size can be determined according to the pores of the porous shell-shaped coral algae, such as 1 cm, 0.5 mm, etc. Of course, corresponding pores can also be drilled on the shell-shaped coral. This method is used to stack the shell-shaped coral algae so that the induction culture medium substrate has sufficient contact surface to fully contact the planktonic larvae, thereby achieving a good induction effect.

[0033] Step 2: Place the planktonic larvae on the bottom of the induction culture medium, then place them in an induction culture container and immerse them in seawater at 27°C for 2-7 days. In this embodiment, the survival rate of the planktonic larvae and the suitable temperature are 27°C. Cultivation under this temperature condition ensures the stability of the planktonic larvae's survival and guarantees a sufficient number of planktonic larvae. In this embodiment, the induction culture container includes a main body with an opening at the top. A cross-shaped partition 14 is bolted and installed inside the main body, dividing the main body into several culture chambers 17. A support part 16 is bolted and installed on the cross-shaped partition 14, and several nozzles 15 are screwed and installed on the support part 16. Each nozzle 15 corresponds to and communicates with a culture chamber 17. The nozzles 15 are flow regulating nozzles. Each culture chamber 17 is connected to an outlet valve 13. The container also includes a pump assembly 19, which is a water pump. The input end of the pump assembly 19 is connected to a reversing valve 11. One inlet of the reversing valve 11 is connected to a seawater source, and the other inlet of the reversing valve 11 is connected to the outlet valve 13 through a pipe 12. The output end of the pump assembly 19 is connected to a heater 18. The heater 18 is connected to several nozzles 15 through a water pipe. A support plate is vertically slidably connected inside the culture chamber 17. An electric control cylinder 21 is fixed on the main body below the support plate. The electric control cylinder 21 is located outside the main body, and the output shaft of the electric control cylinder 21 is bolted to the bottom wall of the support plate. When placing the induction culture medium substrate, the induction culture medium substrate is first raised by the electric control cylinder 21. After the induction culture medium substrate is placed, the induction culture medium substrate is moved to the bottom of the culture chamber 17 by the electric control cylinder 21 (in this way, the chance of damage caused by manual placement can be reduced to a certain extent). Then, seawater at a temperature of 27°C is injected through the pump assembly 19 (at this time, the solenoid valve connects the pump assembly 19 and the seawater source, and the heater 18 is not working). Since the size and shape of the porous shell corals are different, the amount of seawater used is different. Therefore, the flow rate is adjusted by the flow regulating nozzle 15 so that the required amount of seawater can be injected synchronously at the same time. Of course, it is also possible to fill the culture chamber 17 completely, in which case there is no need to adjust the flow rate of the flow regulating nozzle 15.

[0034] Step 3 involves keeping the seawater warm using an induction culture container. After 2-7 days, the seawater is heated again using the induction culture container to continue the induction culture. A heat insulation layer is screwed and installed on the side wall of the main body to prevent heat exchange between the seawater and the outside air, thus achieving the purpose of heat preservation. Alternatively, a heating circulation method can be used, as follows: The pump assembly 19 is connected to the outlet valve 13 via the reversing valve 11. Seawater is then drawn out and passes through the heater 18, which heats the seawater to 27°C. The seawater is then sprayed out through the nozzle 15, thus achieving heat preservation. When seawater heating is required, the pump assembly 19 is connected to the outlet valve 13 via the reversing valve 11. Seawater is then drawn out and passes through the heater 18, which heats the seawater to 30°C. The seawater is then sprayed out through the nozzle 15, thus achieving heating. By using an induction container and controlling the pump assembly 19 and the reversing valve 11, seawater in the culture chamber 17 can be circulated and sprayed out from above the culture chamber 17 through the nozzle 15. On the one hand, the oxygen content in the seawater can be increased by air (similar to oxygenation), reducing the problem of seawater acidification. This can improve the attachment ability of planktonic larvae to a certain extent. On the other hand, the seawater enters the culture chamber 17 through the nozzle 15, causing the seawater to fluctuate and form corresponding waves, similar to the marine environment. This naturally selects for the survival and development of planktonic larvae, thereby increasing the attachment rate of planktonic larvae.

[0035] In this invention, an internal circulation system is used without adding fresh seawater from the outside to ensure the stability of the seawater environment. A stable environment is conducive to improving the attachment ability of planktonic larvae.

[0036] The above descriptions are merely embodiments of the present invention, and common knowledge such as specific structures and / or characteristics in the solutions are not described in detail here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the structure of the present invention, and these should also be considered within the scope of protection of the present invention. These modifications and improvements will not affect the effectiveness of the implementation of the present invention or the practicality of the patent. The scope of protection claimed in this application should be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. A method of inducing planulae of corals to increase their attachment ability, characterized by: It comprises the following steps: Step 1, cut the shell-shaped coral algae to a certain particle size to obtain shell-shaped coral algae particles, and then fill the shell-shaped coral algae particles into the shell-shaped coral algae with holes to obtain an induced culture medium; Step 2, place the planulae on the induced culture medium, then put them into the induced culture container, place the planulae in the holes, and place them in seawater at a temperature of 27℃ for 2-7 days of induced culture; Step 3, keep the seawater warm through the induced culture container, and after 2-7 days, continue the induced culture by warming the seawater through the induced culture container; The induced culture container comprises a body with an open upper portion, a cross-shaped partition plate fixedly installed in the body, the cross-shaped partition plate divides the body into a plurality of culture cavities, a support portion fixedly installed on the cross-shaped partition plate, a plurality of nozzles installed on the support portion, the nozzles correspond to and communicate with the culture cavities one by one, the culture cavities are all communicated with liquid outlet valves, and the induced culture container further comprises a pump assembly, an input end of the pump assembly is communicated with a reversing valve, one inlet of the reversing valve is communicated with a seawater source, the other inlet of the reversing valve is communicated with the liquid outlet valves through a pipeline, an output end of the pump assembly is communicated with a heater, and the heater is communicated with the plurality of nozzles through a water pipe.

2. The method of claim 1, wherein the method is characterized by: In step 1, the filling rate reaches 80-95%.

3. The method of claim 2, wherein the method is for inducing the planula of the coral to have an increased attachment ability. The nozzles are flow-regulating nozzles.

4. The method of claim 3, wherein the method is for inducing the planulae of the coral to have an increased attachment ability. A heat insulation layer is fixedly installed on the side wall of the body.

5. The method of claim 4, wherein the method is for inducing the planulae of the coral to have an increased attachment ability. In step 3, during the induced culture, the pump assembly and the liquid outlet valves are both communicated through the reversing valve, and the pump assembly circulates the water in the culture cavities.

6. The method of claim 5, wherein the method is for inducing the planula of the coral to have an increased attachment ability. In step 3, during the induced culture, the seawater in the culture cavities is fluctuated through the liquid outlet valves and the nozzles to simulate the marine wave environment.

7. The method of claim 6, wherein the method is for inducing the planulae of the coral to have an increased attachment ability. A support plate vertically slidingly connected with the body is arranged in the culture cavity, an electric control cylinder is arranged below the support plate, the electric control cylinder is located outside the body, and an output shaft of the electric control cylinder is fixedly connected with the bottom of the support plate.

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