A detection system based on a filter amplification module and a protein analyzer

Abstract

This invention discloses a detection system and protein analyzer based on a filtering amplification module. The processing module simultaneously provides the same pulse modulation signal to both the light source module and the filtering amplification module. The light source module provides a first wavelength spectrum under a first level signal and is turned off under a second level signal. The photoelectric conversion module converts the second wavelength spectrum into a level signal when the light source module is off. The filtering amplification module is turned off under the first level signal and turned on under the second level signal. Thus, when the light source module is working, the photoelectric conversion module receives both the first and second wavelength spectra. At this time, the filtering amplification module is not turned on. After the light source module is turned off, the second wavelength spectrum is maintained for a certain period, at which point the filtering amplification module turns on. This means that by turning off the light source module, the first wavelength spectrum is filtered out, improving the accuracy of the detection.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of protein detection devices, and particularly relates to a detection system based on a filter amplification module and a protein analyzer. BACKGROUND

[0002] A specific protein analyzer is an auxiliary instrument for analyzing the content and properties of specific proteins in the body. At present, most specific protein analyzers use the principle of turbidimetry and refractometry to measure the concentration of reagents. However, the light disturbance of the protein analysis method based on turbidimetry and refractometry is large, thereby causing low accuracy of the analysis result. SUMMARY

[0003] The present application provides a detection system based on a filter amplification module and a protein analyzer to improve the accuracy of measurement by filtering out a first wavelength spectrum.

[0004] In a first aspect, an embodiment of the present application provides a detection system based on a filter amplification module, comprising a processing module, a filter amplification module, a light source module, and a photoelectric conversion module.

[0005] The output end of the processing module is electrically connected to the input end of the light source module and the control end of the filter amplification module, for simultaneously providing the same pulse modulation signal to the light source module and the filter amplification module, wherein the pulse modulation signal comprises a first level signal and a second level signal, and the first level signal is greater than the second level signal.

[0006] The light source module is used to provide a first wavelength spectrum under the action of the first level signal, and is turned off under the action of the second level signal.

[0007] The sample module is used to convert the first wavelength spectrum into a second wavelength spectrum.

[0008] The photoelectric conversion module is used to convert the second wavelength spectrum into a level signal when the light source module is turned off.

[0009] The input end of the filter amplification module is electrically connected to the output end of the photoelectric conversion module, the output end of the filter amplification module is electrically connected to the processing module, and the filter amplification module is used to be disconnected under the action of the first level signal and be turned on under the action of the second level signal, so that the level signal input by the photoelectric conversion module is output to the processing module after being filtered and amplified by the filter amplification module.

[0010] Further, the filter amplification module comprises a first amplification circuit, an integration circuit, a control circuit, and a second amplification circuit.

[0011] The inverting input end of the first amplification circuit is electrically connected with the output end of the photoelectric conversion module, the output end of the first amplification circuit is electrically connected with the input end of the integration circuit, the output end of the integration circuit is electrically connected with the input end of the control circuit and the non-inverting input end of the second amplification circuit respectively, the output end of the second amplification circuit is electrically connected with the input end of the processing module, the control end of the control circuit is electrically connected with the input end of the processing module, and the control module is used for being turned on under the action of the first level signal to make the integration circuit and the second amplification circuit disconnected, and being turned off under the action of the second level signal to make the integration circuit and the second amplification circuit connected.

[0012] Further, the first amplification circuit comprises a first operational amplifier, the integration circuit comprises a first resistor and a first capacitor, the second amplification circuit comprises a second operational amplifier, and the control circuit comprises a triode.

[0013] The inverting input end of the first operational amplifier is electrically connected with the output end of the photoelectric conversion module, the non-inverting input end of the first operational amplifier is electrically connected with a ground end, the output end of the first operational amplifier is electrically connected with the first end of the first resistor, the second end of the first resistor is electrically connected with the first plate of the first capacitor, the second plate of the first capacitor is electrically connected with the collector of the triode and the non-inverting input end of the second operational amplifier respectively, and the output end of the second operational amplifier is electrically connected with the input end of the processing module.

[0014] The emitter of the triode is electrically connected with the ground end, and the base of the triode is electrically connected with the input end of the processing module.

[0015] Further, the control circuit further comprises a second resistor and a third resistor.

[0016] The first end of the second resistor is electrically connected with the input end of the processing module, the second end of the second resistor is electrically connected with the first end of the third resistor and the base of the triode respectively, and the second end of the third resistor is electrically connected with the ground end.

[0017] The first amplification circuit further comprises a fourth resistor, the second amplification circuit further comprises a fifth resistor and a sixth resistor.

[0018] The first end of the fourth resistor is electrically connected with the output end of the photoelectric conversion module, the second end of the fourth resistor is electrically connected with the inverting input end of the first operational amplifier, the first end of the fifth resistor is electrically connected with the second plate of the first capacitor, the second end of the fifth resistor is electrically connected with the non-inverting input end of the second operational amplifier, and the sixth resistor is connected in series between the output end of the second operational amplifier and the input end of the processing module.

[0019] Furthermore, the filtering and amplification module also includes a first isolation circuit, which is located between the output terminal of the processing module and the control terminal of the control circuit, and the first isolation circuit includes a first optocoupler;

[0020] The first end of the first optocoupler is electrically connected to the output end of the processing module, the second end of the first optocoupler is electrically connected to the ground end, the third end of the first optocoupler is electrically connected to the control end of the control circuit, and the fourth end of the first optocoupler is electrically connected to the power supply voltage end.

[0021] Furthermore, the detection system also includes a second isolation circuit, which is located between the output terminal of the processing module and the input terminal of the light source module, and the second isolation circuit includes a second optocoupler;

[0022] The first end of the second optocoupler is electrically connected to the output end of the processing module, the second end of the second optocoupler is electrically connected to the ground end, the third end of the second optocoupler is electrically connected to the input end of the light source module, and the fourth end of the second optocoupler is electrically connected to the power supply voltage end.

[0023] Furthermore, the detection system also includes an analog-to-digital conversion module, which is located between the output of the filter amplifier circuit and the input of the processing module.

[0024] Furthermore, the detection system also includes a display module, which is electrically connected to the output of the processing module. The display module is used to display image information generated by the processing module based on the level signal of the filtering and amplification module.

[0025] Secondly, embodiments of the present invention also provide a protein analyzer, including the detection system provided in any embodiment of the present invention.

[0026] The detection system based on a filtering amplification module in this invention includes a processing module, a filtering amplification module, a light source module, and a photoelectric conversion module. The output of the processing module is electrically connected to the input of the light source module and the control terminal of the filtering amplification module, respectively, to simultaneously provide the same pulse modulation signal to both the light source module and the filtering amplification module. The pulse modulation signal includes a first-level signal and a second-level signal, with the first-level signal being greater than the second-level signal. The light source module provides a first-wavelength spectrum under the first-level signal and is turned off under the second-level signal. The sample module converts the first-wavelength spectrum into a second-wavelength spectrum, and the photoelectric conversion module converts the second-wavelength spectrum into a level signal when the light source module is off. The input of the filtering amplification module is electrically connected to the output of the photoelectric conversion module, and the output of the filtering amplification module is electrically connected to the processing module. The filtering amplification module is turned off under the first-level signal and turned on under the second-level signal, so that the level signal input to the photoelectric conversion module is filtered, amplified, and output to the processing module. In the above technical solution, the light source module and the filtering and amplification module are controlled by the same pulse modulation information. When the light source module is working, it provides a first wavelength spectrum, which is converted into a second wavelength spectrum by the sample module. That is, at this time, the photoelectric conversion module includes both the first and second wavelength spectra. Since the filtering and amplification module is disconnected, the influence of the first wavelength spectrum on the detection is avoided. When the light source module is turned off, the second wavelength spectrum will be maintained for a certain period of time, meaning that the photoelectric conversion module includes the second wavelength spectrum. At this time, the filtering and amplification module is turned on, and the level signal input to the photoelectric conversion module is filtered, amplified, and output to the processing module. This detection system has higher sensitivity and anti-interference ability by filtering out the influence of the first wavelength spectrum on the detection at the source, making the detection results more accurate. Attached Figure Description

[0027] Figure 1 This is a structural block diagram of a detection system based on a filtering and amplification module provided in an embodiment of the present invention;

[0028] Figure 2 This is a partial circuit structure diagram of a detection system based on a filter amplification module provided in an embodiment of the present invention. Detailed Implementation

[0029] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of this invention will be fully described below with reference to the accompanying drawings in the embodiments of this invention, through specific implementation methods. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. All other embodiments obtained by those skilled in the art based on the embodiments of this invention without creative effort fall within the protection scope of this invention.

[0030] It should be noted that the terms "first," "second," etc., in the specification, claims, and accompanying drawings of this invention are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be interchanged where appropriate so that the embodiments of the invention described herein can be implemented in orders other than those illustrated or described herein. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover a non-exclusive inclusion; for example, a process, method, system, product, or apparatus that comprises a series of steps or units is not necessarily limited to those steps or units explicitly listed, but may include other steps or units not explicitly listed or inherent to such processes, methods, products, or apparatus.

[0031] Figure 1 This is a structural block diagram of a detection system based on a filtering and amplification module provided in an embodiment of the present invention, as shown below. Figure 1 As shown, the detection system includes a processing module 10, a filtering and amplification module 20, a light source module 30, and a photoelectric conversion module 40. The output of the processing module 10 is electrically connected to the input of the light source module 30 and the control terminal of the filtering and amplification module 20, respectively, to simultaneously provide the same pulse modulation signal (PWM) to both the light source module 30 and the filtering and amplification module 20. The PWM signal includes a first level signal and a second level signal, with the first level signal being greater than the second level signal. The light source module 30 provides a first wavelength spectrum under the action of the first level signal and is turned off under the action of the second level signal. The sample module 01 converts the first wavelength spectrum into a second wavelength spectrum. The photoelectric conversion module 40 converts the second wavelength spectrum into a level signal (PMT) when the light source module 30 is turned off. The input of the filtering and amplification module 20 is electrically connected to the output of the photoelectric conversion module 40, and the output of the filtering and amplification module 20 is electrically connected to the processing module 10. The filtering and amplification module 20 is turned off under the action of the first level signal and turned on under the action of the second level signal, so that the level signal (PMT) input to the photoelectric conversion module 40 is output to the processing module 10 after passing through the filtering and amplification module 20.

[0032] Specifically, the light source module 30 may include a laser light-emitting diode. The output terminal of the processing module 10 is electrically connected to the input terminal of the light source module 30, thereby allowing the processing module 10 to provide a light emission control signal to the light source module 30 to emit light. The pulse modulation signal (PWM) includes a first level signal and a second level signal that change at continuous intervals. The first level signal can be a high level signal, and the second level signal can be a low level signal. When the processing module 10 provides the PWM signal to the light source module 30, under the action of the first level signal, the light source module 30 emits light to provide a first wavelength spectrum; under the action of the second level signal, the light source module 30 does not emit light. In other words, under the action of the PWM signal, the light source module 30 maintains intermittent light emission. The sample module 01 is the sample to be detected, for example, the sample module 01 can be a protein sample, and the detection system can then detect the content and properties of specific proteins within the protein sample. Sample module 01 is placed in the light output path of light source module 30 so that the first wavelength spectrum emitted by light source module 30 illuminates sample module 01. Then, under the action of pulse modulation signal PWM, the first wavelength spectrum provided by light source module 30 at intervals reacts chemically with sample module 01 to generate a second wavelength spectrum. Photoelectric conversion module 40 is positioned in the light output path of the second wavelength spectrum. When light source module 30 is operating, the receiving end of photoelectric conversion module 40 includes composite light (i.e., the second wavelength spectrum generated through the chemical reaction, and the first wavelength spectrum provided by light source module 30). When light source module 30 is turned off, since the second wavelength spectrum is maintained for a certain period, the receiving end of photoelectric conversion module 40 also includes the second wavelength spectrum generated by the chemical reaction.

[0033] It should be noted that in the detection and analysis, the second wavelength spectrum is used for the detection of sample module 01, while the first wavelength spectrum will interfere with the detection of sample module 01. Therefore, it is necessary to filter out the influence of the first wavelength spectrum, that is, the light signal when the light source module 30 is turned off needs to be used as the analysis signal of the processing module 10.

[0034] Therefore, in this embodiment of the invention, the output terminal of the processing module 10 is electrically connected to the input terminal of the light source module 30 and the control terminal of the filter amplification module 20, respectively. The processing module 10 simultaneously provides the same pulse modulation signal (PWM) to both the light source module 30 and the filter amplification module 20. Under the action of the first level signal, the filter amplification module 20 is cut off, and the processing module 10 cannot detect the sample module 01. Under the action of the second level signal, the filter amplification module 20 is turned on, and the processing module 01 can detect the sample module 01. This can be understood as follows: under the action of the first level signal, the light source module 30 emits light to provide a first wavelength spectrum, and the photoelectric conversion module 4... The receiving end of sample module 01 receives composite light (i.e., the second wavelength spectrum generated through a chemical reaction and the first wavelength spectrum provided by the light source module 30). At this time, the filter amplification module 20 is turned off, and the processing module 10 cannot detect sample module 01. However, under the action of the second level signal, the light source module 30 is turned off, and the receiving end of the photoelectric conversion module 40 receives the second wavelength spectrum. The photoelectric conversion module 40 converts the second wavelength spectrum into a level signal PMT and transmits it to the filter amplification module 20. At this time, the filter amplification module 20 is turned on, and the level signal PMT is filtered and amplified by the filter amplification module 20 before being transmitted to the processing module 10, whereby the processing module 10 begins detection. In this way, by filtering out the interference of the first wavelength spectrum at the source, the detection system has higher sensitivity and anti-interference ability, making the detection results more accurate.

[0035] It should be noted that when the light source module 30 is turned on, the receiving end of the photoelectric conversion module 40 receives composite light. Although the photoelectric conversion module 40 can convert composite light into an electrical signal, since the filter amplification module 20 is disconnected, the electrical signal cannot be transmitted to the processing module 10. Therefore, to better demonstrate the inventive point of this case, the photoelectric conversion module 40 is limited to converting the second wavelength spectrum into a level signal PMT when the light source module 30 is turned off. Those skilled in the art will understand that the photoelectric conversion module 40 itself is used to convert optical signals into electrical signals, that is, the photoelectric conversion module 40 can also convert composite light into a level signal.

[0036] It should also be noted that the first wavelength spectrum can be an excitation spectrum of 680 nm and the second wavelength spectrum can be an emission spectrum of 610 nm. The embodiments of the present invention do not limit the specific data of the first wavelength spectrum and the second wavelength spectrum, and those skilled in the art can set them as needed.

[0037] In summary, the detection system based on the filtering amplification module in this invention includes a processing module, a filtering amplification module, a light source module, and a photoelectric conversion module. The output of the processing module is electrically connected to the input of the light source module and the control terminal of the filtering amplification module, respectively, to simultaneously provide the same pulse modulation signal to both the light source module and the filtering amplification module. The pulse modulation signal includes a first-level signal and a second-level signal, with the first-level signal being greater than the second-level signal. The light source module provides a first wavelength spectrum under the influence of the first-level signal and is turned off under the influence of the second-level signal. The sample module converts the first wavelength spectrum into a second wavelength spectrum, and the photoelectric conversion module converts the second wavelength spectrum into a level signal when the light source module is off. The input of the filtering amplification module is electrically connected to the output of the photoelectric conversion module, and the output of the filtering amplification module is electrically connected to the processing module. The filtering amplification module is turned off under the influence of the first-level signal and turned on under the influence of the second-level signal, so that the level signal input to the photoelectric conversion module is filtered, amplified, and output to the processing module. In the above technical solution, the light source module and the filtering and amplification module are controlled by the same pulse modulation information. When the light source module is working, it provides a first wavelength spectrum, which is converted into a second wavelength spectrum by the sample module. That is, at this time, the photoelectric conversion module includes both the first and second wavelength spectra. Since the filtering and amplification module is disconnected, the influence of the first wavelength spectrum on the detection is avoided. When the light source module is turned off, the second wavelength spectrum will be maintained for a certain period of time, meaning that the photoelectric conversion module includes the second wavelength spectrum. At this time, the filtering and amplification module is turned on, and the level signal input to the photoelectric conversion module is filtered, amplified, and output to the processing module. This detection system has higher sensitivity and anti-interference ability by filtering out the influence of the first wavelength spectrum on the detection at the source, making the detection results more accurate.

[0038] Optional, Figure 2 This is a partial circuit structure diagram of a detection system based on a filter amplification module provided in an embodiment of the present invention. See also... Figure 1 and Figure 2The filter amplification module 20 includes a first amplification circuit 210, an integrator circuit 220, a control circuit 230, and a second amplification circuit 240. The inverting input terminal of the first amplification circuit 210 is electrically connected to the output terminal of the photoelectric conversion module 40. The output terminal of the first amplification circuit 210 is electrically connected to the input terminal of the integrator circuit 220. The output terminal of the integrator circuit 220 is electrically connected to both the input terminal of the control circuit 240 and the non-inverting input terminal of the second amplification circuit 240. The output terminal of the second amplification circuit 240 is electrically connected to the input terminal of the processing module 10. The control terminal of the control circuit 230 is electrically connected to the output terminal of the processing module 10. The control module 240 is used to turn on under the action of a first level signal to disconnect the integrator circuit 220 from the second amplification circuit 240, and to turn off under the action of a second level signal to turn the integrator circuit 220 and the second amplification circuit 240 on.

[0039] Specifically, the first amplifier circuit 210 is located at the output end of the photoelectric conversion module 40. That is, the inverting input terminal of the first amplifier circuit 210 is electrically connected to the output terminal of the photoelectric conversion module 40, and the output terminal of the first amplifier circuit 210 is electrically connected to the inverting input terminal of the first amplifier circuit 210. In this way, the first amplifier circuit 210 forms a follower circuit, which buffers and isolates the level signal output by the photoelectric conversion module 40 and improves the load capacity of the circuit. The input terminal of the integrating circuit 220 is electrically connected to the output terminal of the first amplifying circuit 210, and the output terminal of the integrating circuit 220 is electrically connected to the control circuit 230 and the second amplifying circuit 240 respectively. Thus, under the action of the first level signal, the light source module 40 emits light to provide the first wavelength spectrum, and the control circuit 230 is turned on. At this time, the first amplifying circuit 210, the integrating circuit 220 and the control circuit 230 are connected. The receiving end of the photoelectric conversion module 40 includes composite light. The level signal converted by the photoelectric conversion module 40 is accumulated in the integrating circuit 220 and is not output to the second amplifying circuit 240, that is, the processing module 10 does not perform detection.

[0040] Under the action of the second level signal, the light source module 40 is turned off and the control circuit 230 is cut off. At this time, the first amplification circuit 210, the integrator circuit 220 and the second amplification circuit 240 are connected. The receiving end of the photoelectric conversion module 40 includes the second wavelength spectrum. Then, the photoelectric conversion module 40 converts the second wavelength spectrum into a level signal. This level signal is buffered and isolated by the first amplification circuit 240, amplified by the integrator circuit 220 and the second amplification circuit 240 and output to the input end of the processing module 10. The processing module 10 starts to perform detection. In this way, the control circuit 230 and the light source module 30 are controlled simultaneously by the same pulse modulation signal PWM. When the light source module 30 is turned off, the control circuit 230 is cut off so that the filter amplification module 20 is turned on to perform signal analysis. By filtering out the influence of the first wavelength spectrum on the detection at the source, it has higher sensitivity and anti-interference ability, making the detection results more accurate.

[0041] Optionally, based on the above embodiments, see also... Figure 1 and Figure 2 The first amplifier circuit 210 includes a first operational amplifier U1, the integrating circuit 220 includes a first resistor R1 and a first capacitor C1, the second amplifier circuit 240 includes a second operational amplifier U2, and the control circuit 230 includes a transistor Q1. The inverting input of the first operational amplifier U1 is electrically connected to the output of the photoelectric conversion module 40, the non-inverting input of the first operational amplifier U1 is electrically connected to ground GND, the output of the first operational amplifier U1 is electrically connected to the first end of the first resistor R1, the second end of the first resistor R1 is electrically connected to the first plate of the first capacitor C1, the second plate of the first capacitor C1 is electrically connected to the collector of the transistor Q1 and the non-inverting input of the second operational amplifier U2, and the output of the second operational amplifier U2 is electrically connected to the input of the processing module 10. The emitter of the transistor Q1 is electrically connected to ground GND, and the base of the transistor Q1 is electrically connected to the input of the processing module 10.

[0042] Specifically, the output terminal of the processing module 10 is electrically connected to the base of the transistor Q1. Under the action of the first level signal, the transistor Q1 is turned on. At this time, the light source module 30 emits light to provide the first wavelength spectrum. The receiving end of the photoelectric conversion module 40 includes composite light. The level signal converted by the photoelectric conversion module 40 is buffered and isolated at the first operational amplifier U1 and then reaches the first resistor R1. Since the second plate of the first capacitor C1 is electrically connected to the ground terminal GND through the transistor Q1 after the transistor Q1 is turned on, the level signal converted by the photoelectric conversion module 40 accumulates at the second plate of the first capacitor C1 and is not output to the second operational amplifier U2. That is, at this time the filter amplification module 20 is disconnected and the processing module 10 does not perform detection. Under the influence of the second level signal, transistor Q1 is cut off. At this time, the light source module 30 is turned off, and the receiving end of the photoelectric conversion module 40 includes the second wavelength spectrum. The level signal converted by the photoelectric conversion module 40 is then buffered and isolated at the first operational amplifier U1 before reaching the first resistor R1. Since the second plate of the first capacitor C1 is electrically connected to the non-inverting input of the second operational amplifier U2 after transistor Q1 is cut off, the level signal converted by the photoelectric conversion module 40 is amplified by the second operational amplifier U2 and output to the input of the processing module 10. At this time, the filter amplification module 20 is turned on, and the processing module 10 begins detection. Thus, by filtering out the influence of the first wavelength spectrum on the detection at the source, higher sensitivity and anti-interference capability are achieved, resulting in more accurate detection results.

[0043] Optionally, based on the above embodiments, see also... Figure 1 and Figure 2 The control circuit 230 also includes a second resistor R2 and a third resistor R3. The first end of the second resistor R2 is electrically connected to the input terminal of the processing module 10, and the second end of the second resistor R2 is electrically connected to the first end of the third resistor R3 and the base of the transistor Q1. The second end of the third resistor R3 is electrically connected to the ground terminal GND. The first amplifier circuit 210 also includes a fourth resistor R4, and the second amplifier circuit 240 also includes a fifth resistor R5 and a sixth resistor R6. The first end of the fourth resistor R4 is electrically connected to the output terminal of the photoelectric conversion module 40, and the second end of the fourth resistor R4 is electrically connected to the inverting input terminal of the first operational amplifier U1. The first end of the fifth resistor R5 is electrically connected to the second plate of the first capacitor C1, and the second end of the fifth resistor R5 is electrically connected to the non-inverting input terminal of the second operational amplifier U2. The sixth resistor R6 is connected in series between the output terminal of the second operational amplifier U2 and the input terminal of the processing module 10.

[0044] Specifically, the second resistor R2 and the third resistor R3 are placed between the base and emitter of transistor Q1, thereby setting the turn-on voltage of transistor Q1. The fourth resistor R4 is placed between the output of the photoelectric conversion module 40 and the inverting input of the first operational amplifier U1. The fifth resistor R5 is placed between the second plate of the first capacitor C1 and the non-inverting input of the second operational amplifier U2. The sixth resistor R6 is placed between the output of the second operational amplifier U2 and the input of the processing module 10. These resistors act as a voltage divider, thus protecting the circuit. It should be noted that the first amplifier circuit 210, the integrating circuit 220, the control circuit 230, and the second amplifier circuit 240 also include other resistors and capacitors to ensure the normal operation of the various components in the circuit; these will not be described in detail here.

[0045] Optional, see below Figure 1 and Figure 2 The filtering and amplification module 20 also includes a first isolation circuit 250, located between the output terminal of the processing module 10 and the control terminal of the control circuit 230. The first isolation circuit 250 includes a first optocoupler U3. The first terminal of the first optocoupler U3 is electrically connected to the output terminal of the processing module 10, the second terminal of the first optocoupler U3 is electrically connected to the ground terminal GND, the third terminal of the first optocoupler U3 is electrically connected to the control terminal of the control circuit 230, and the fourth terminal of the first optocoupler U3 is electrically connected to the power supply voltage terminal VCC. Specifically, the pulse modulation signal PWM includes continuously changing first and second level information. By setting the first isolation circuit 250 between the output terminal of the processing module 10 and the control terminal of the control circuit 230, the first isolation circuit 250 has strong anti-interference capabilities, thus reducing the influence of the pulse modulation signal PWM on the measurement results. Furthermore, the photoelectric conversion module 40 itself also has a large current; therefore, the influence of the photoelectric conversion module 40 can also be reduced through the first isolation circuit 250. It should be noted that the first isolation circuit 250 also includes some pull-up resistors and pull-down resistors to ensure the normal operation of the first optocoupler U3. These will not be described in detail in this embodiment of the invention.

[0046] Optional, see below Figure 1 and Figure 2The detection system also includes a second isolation circuit 50, located between the output of the processing module 10 and the input of the light source module 30. The second isolation circuit includes a second optocoupler U4. The first end of the second optocoupler U4 is electrically connected to the output of the processing module 10, the second end is electrically connected to the ground terminal GND, the third end is electrically connected to the input of the light source module 30, and the fourth end is electrically connected to the power supply voltage terminal VCC. Specifically, the pulse modulation signal PWM includes continuously changing first and second level information. By setting the second isolation circuit 50 between the output of the processing module 10 and the input of the light source module 30, the second isolation circuit 50 has strong anti-interference capabilities, thus reducing the influence of the pulse modulation signal PWM on the measurement results. Furthermore, the photoelectric conversion module 40 itself also has a large current; therefore, the influence of the photoelectric conversion module 40 can also be reduced through the second isolation circuit 50. It should be noted that the second isolation circuit 50 also includes some pull-up resistors and pull-down resistors to ensure the normal operation of the second optocoupler U4. These will not be described in detail in this embodiment of the invention.

[0047] Optional, see below Figure 1 The detection system also includes a filter 60, which is positioned in the optical path between the sample module 01 and the photoelectric conversion module 40. The filter 60 is used to filter out the first wavelength spectrum. Specifically, the filter 60 can be a bandpass filter, allowing the second wavelength spectrum to pass through while filtering out other wavelength ranges. The photoelectric conversion module 40 can be a photomultiplier tube. Under the action of the second level signal, the light source module 30 is turned off, and the second wavelength spectrum generated at the sample module 01 reaches the photoelectric conversion module 40 through the filter 60, further reducing the influence of the first wavelength spectrum on the measurement results and further improving the accuracy of the measurement.

[0048] Optional, see below Figure 1 The detection system also includes an analog-to-digital converter (ADC) module 70, located between the output of the filter amplifier circuit 20 and the input of the processing module 10. Specifically, the filter amplifier circuit 20 outputs a level signal, i.e., an analog signal. Since the processing module 10 can be a processing chip, it cannot directly process and display the analog signal. Therefore, by placing the ADC module 70 at the output of the filter amplifier circuit 20 and the input of the processing module 10, the ADC module 70 converts the analog signal into a digital signal, ensuring that the processing module 10 can perform detection.

[0049] Optional, see below Figure 1The detection system also includes a display module 80, which is electrically connected to the output of the processing module 10. The display module 80 is used to display the image information generated by the processing module 10 based on the level signal from the filtering and amplifying module 20. Specifically, the level signal output by the filtering and amplifying module 20 is converted into a digital signal by the analog-to-digital converter 70 and then output to the processing module 10. The processing module 10 then begins detection and displays the final detection result through the display module 80, allowing the user to intuitively understand the detection result.

[0050] Optional, see below Figure 1 The detection system also includes a parameter adjustment module 90, which is electrically connected to the input terminal of the processing module 10 and is used to adjust the parameter information of the pulse modulation signal (PWM). Specifically, the frequency and duty cycle of the PWM signal can be set through the parameter adjustment module 90. Furthermore, by changing the frequency and duty cycle of the PWM signal, multiple detections can be performed to improve the accuracy of the detection.

[0051] Based on the same inventive concept, embodiments of the present invention also provide a protein analyzer, which includes the detection system described in any of the above embodiments. Therefore, the protein analyzer provided by the embodiments of the present invention has the corresponding beneficial effects of the above embodiments, which will not be repeated here.

[0052] Note that the above description is merely a preferred embodiment of the present invention and the technical principles employed. Those skilled in the art will understand that the present invention is not limited to the specific embodiments described herein, and various obvious changes, readjustments, combinations, and substitutions can be made without departing from the scope of protection of the present invention. Therefore, although the present invention has been described in detail through the above embodiments, the present invention is not limited to the above embodiments, and may include many other equivalent embodiments without departing from the concept of the present invention, the scope of which is determined by the scope of the appended claims.

Claims

1. A detection system based on a filtering and amplification module, characterized in that, It includes a processing module, a filtering and amplification module, a light source module, and a photoelectric conversion module; The output terminal of the processing module is electrically connected to the input terminal of the light source module and the control terminal of the filtering and amplifying module, respectively, for simultaneously providing the same pulse modulation signal to the light source module and the filtering and amplifying module, wherein the pulse modulation signal includes a first level signal and a second level signal, and the first level signal is greater than the second level signal; The light source module is used to provide a first wavelength spectrum under the action of the first level signal and to turn off under the action of the second level signal; The sample module is used to convert the first wavelength spectrum into a second wavelength spectrum; The photoelectric conversion module is used to convert the second wavelength spectrum into a level signal when the light source module is turned off; The input terminal of the filtering and amplifying module is electrically connected to the output terminal of the photoelectric conversion module, and the output terminal of the filtering and amplifying module is electrically connected to the processing module. The filtering and amplifying module is used to disconnect under the action of the first level signal and to conduct under the action of the second level signal, so that the level signal input by the photoelectric conversion module is output to the processing module after passing through the filtering and amplifying module.

2. The detection system according to claim 1, characterized in that, The filtering and amplification module includes a first amplification circuit, an integration circuit, a control circuit, and a second amplification circuit. The inverting input terminal of the first amplifier circuit is electrically connected to the output terminal of the photoelectric conversion module. The output terminal of the first amplifier circuit is electrically connected to the input terminal of the integrator circuit. The output terminal of the integrator circuit is electrically connected to the input terminal of the control circuit and the non-inverting input terminal of the second amplifier circuit. The output terminal of the second amplifier circuit is electrically connected to the input terminal of the processing module. The control terminal of the control circuit is electrically connected to the output terminal of the processing module. The control circuit is used to turn on under the action of the first level signal to disconnect the integrator circuit from the second amplifier circuit, and to turn off under the action of the second level signal to turn the integrator circuit from the second amplifier circuit.

3. The detection system according to claim 2, characterized in that, The first amplifier circuit includes a first operational amplifier, the integrator circuit includes a first resistor and a first capacitor, the second amplifier circuit includes a second operational amplifier, and the control circuit includes a transistor; The inverting input terminal of the first operational amplifier is electrically connected to the output terminal of the photoelectric conversion module, the non-inverting input terminal of the first operational amplifier is electrically connected to the ground terminal, the output terminal of the first operational amplifier is electrically connected to the first terminal of the first resistor, the second terminal of the first resistor is electrically connected to the first plate of the first capacitor, the second plate of the first capacitor is electrically connected to the collector of the transistor and the non-inverting input terminal of the second operational amplifier, and the output terminal of the second operational amplifier is electrically connected to the input terminal of the processing module. The emitter of the transistor is electrically connected to the ground terminal, and the base of the transistor is electrically connected to the input terminal of the processing module.

4. The detection system according to claim 3, characterized in that, The control circuit also includes a second resistor and a third resistor; The first end of the second resistor is electrically connected to the input terminal of the processing module, the second end of the second resistor is electrically connected to the first end of the third resistor and the base of the transistor, and the second end of the third resistor is electrically connected to the ground terminal. The first amplifier circuit further includes a fourth resistor, and the second amplifier circuit further includes a fifth resistor and a sixth resistor; The first end of the fourth resistor is electrically connected to the output end of the photoelectric conversion module, the second end of the fourth resistor is electrically connected to the inverting input end of the first operational amplifier, the first end of the fifth resistor is electrically connected to the second plate of the first capacitor, the second end of the fifth resistor is electrically connected to the non-inverting input end of the second operational amplifier, and the sixth resistor is connected in series between the output end of the second operational amplifier and the input end of the processing module.

5. The detection system according to claim 2, characterized in that, The filtering and amplification module further includes a first isolation circuit, which is located between the output terminal of the processing module and the control terminal of the control circuit. The first isolation circuit includes a first optocoupler. The first end of the first optocoupler is electrically connected to the output end of the processing module, the second end of the first optocoupler is electrically connected to the ground end, the third end of the first optocoupler is electrically connected to the control end of the control circuit, and the fourth end of the first optocoupler is electrically connected to the power supply voltage end.

6. The detection system according to claim 1, characterized in that, The detection system further includes a second isolation circuit, which is located between the output terminal of the processing module and the input terminal of the light source module. The second isolation circuit includes a second optocoupler. The first end of the second optocoupler is electrically connected to the output end of the processing module, the second end of the second optocoupler is electrically connected to the ground terminal, the third end of the second optocoupler is electrically connected to the input end of the light source module, and the fourth end of the second optocoupler is electrically connected to the power supply voltage terminal.

7. The detection system according to claim 1, characterized in that, The detection system also includes a filter, which is disposed in the optical path between the sample module and the photoelectric conversion module, and is used to filter out the first wavelength spectrum.

8. The detection system according to claim 1, characterized in that, The detection system also includes an analog-to-digital conversion module, which is located between the output of the filtering and amplifying module and the input of the processing module.

9. The detection system according to claim 1, characterized in that, The detection system also includes a display module, which is electrically connected to the output of the processing module. The display module is used to display image information generated by the processing module based on the level signal of the filtering and amplification module.

10. A protein analyzer, characterized in that, The detection system includes any one of claims 1-9.

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